Publications by authors named "M Alejandra Romero"

1,854 Publications

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Platinum(IV)-azido monocarboxylato complexes are photocytotoxic under irradiation with visible light.

Dalton Trans 2021 Jul 19. Epub 2021 Jul 19.

Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, UK. and Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.

Complexes trans,trans,trans-[Pt(N3)2(OH)(OCOR)(py)2] where py = pyridine and where OCOR = succinate (1); 4-oxo-4-propoxybutanoate (2) and N-methylisatoate (3) have been synthesized by derivation of trans,trans,trans-[Pt(OH)2(N3)2(py)2] (4) and characterised by NMR and EPR spectroscopy, ESI-MS and X-ray crystallography. Irradiation of 1-3 with green (517 nm) light initiated photoreduction to Pt(ii) and release of the axial ligands at a 3-fold faster rate than for 4. TD-DFT calculations showed dissociative transitions at longer wavelengths for 1 compared to 4. Complexes 1 and 2 showed greater photocytotoxicity than 4 when irradiated with 420 nm light (A2780 cell line IC50 values: 2.7 and 3.7 μM) and complex 2 was particularly active towards the cisplatin-resistant cell line A2780cis (IC50 3.7 μM). Unlike 4, complexes 1-3 were phototoxic under green light irradiation (517 nm), with minimal toxicity in the dark. A pKa(H2O) of 5.13 for the free carboxylate group was determined for 1, corresponding to an overall negative charge during biological experiments, which crucially, did not appear to impede cellular accumulation and photocytotoxicity.
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http://dx.doi.org/10.1039/d1dt01730fDOI Listing
July 2021

Efficacy and safety of cerebral embolic protection systems during transcatheter aortic valve replacement: a review of current clinical findings.

Expert Rev Cardiovasc Ther 2021 Jul 21:1-13. Epub 2021 Jul 21.

CVPath Institute, Gaithersburg, MD, USA.

Introduction: Cerebrovascular events are one of the most serious consequences after transcatheter aortic valve replacement (TAVR). More than half of the cerebrovascular events following TAVR are due to procedure-related emboli. Embolic protection devices (EPDs) have the potential to decrease cerebrovascular events during TAVR procedure. However, randomized controlled trials (RCTs) have not conclusively determined if EPDs are effective, likely because of small number of patients enrolled. However, meta-analyses and propensity-matched analyses from large registries have shown efficacy and suggest the importance of EPDs in prevention of stroke during TAVR and perhaps other structural heart procedures.

Areas Covered: This review will focus on clinical and histopathologic studies examining the efficacy, safety, and histopathologic device capture findings in the presence and absence of EPDs during TAVR procedures.

Expert Opinion: Clinical studies have not conclusively determined the efficacy of EPDs. Current ongoing large-scale RCT (PROTECTED TAVR [NCT04149535]) has the potential to prove their efficacy. Histopathological evaluation of debris captured by EPDs contributes to our understanding of the mechanisms of TAVR procedure-related embolic events and suggests the importance of preventing cerebral embolization of debris released during this and other structural heart procedures.
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http://dx.doi.org/10.1080/14779072.2021.1955346DOI Listing
July 2021

One-Pot Microwave-Assisted Synthesis of Carbon Dots and and Antimicrobial Photodynamic Applications.

Front Microbiol 2021 21;12:662149. Epub 2021 Jun 21.

São Carlos Institute of Physics, University of São Paulo, São Carlos, Brazil.

Carbon-based photosensitizers are more attractive than the other ones based on their low cost, high stability, broadband of light absorption, tunable emission spectra, high quantum yield, water solubility, high resistance to metabolic degradation, and selective delivery. These properties allow multiple applications in the field of biology and medicine. The present study evaluated and the antimicrobial photodynamic effect of a one-pot microwave produced C-DOTS based on citric acid. The assays assessed the effectiveness of illuminated C-DOTS (C-DOTS + light) against suspension and biofilm. The concentrations of 6.9 and 13.8 mg/mL of C-DOTS and light doses of 20 and 40 J/cm were able to reduce significantly the microorganisms. Based on these parameters and results, the experiments were conducted in mice, evaluating this treatment on wounds contaminated with The viability test showed that C-DOTS-mediated photodynamic inactivation reduced 10 log of the bacteria present on the skin lesions. These results, altogether, showed that antibacterial photodynamic therapy using C-DOTS is a promising and viable treatment for Gram-positive bacteria-infected wounds.
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http://dx.doi.org/10.3389/fmicb.2021.662149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255795PMC
June 2021

Peer education: youth protagonism in a preventive approach to alcohol and other drugs.

Cien Saude Colet 2021 Jul 2;26(7):2759-2768. Epub 2021 Apr 2.

Diretoria Regional de Brasília, Fundação Oswaldo Cruz. Campus Universitário Darcy Ribeiro, Asa Norte. 70750540 Brasília DF Brasil.

This article describes the experience of the course in Health and Safety at School, which prepared young people to spread knowledge on prevention of alcohol and other drugs, using the peer education methodology. The participants were sixty 15 to 19 year-old upper secondary school students from 8 public schools in the Federal District. The activity was structured into 8 modules, with each module consisting of a meeting to discuss content, followed by field work at the schools, where students trained their colleagues and passed on information to them; and, lastly, a further meeting for the students to give feedback on their experience in the field. All activities were recorded in field diaries, photographs, and reports, providing input to this experience report. Finally, a student forum was held at which the young people presented projects to be carried out at the schools. The peer education experience enabled the young people to take the lead in their school community and territory, and acknowledged the value of sharing among people with similar experiences. It thus favoured the development of critical, artistically potent strategies for preventive action directed to adolescents in situations of social vulnerability and fostered health promotion in the school.
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http://dx.doi.org/10.1590/1413-81232021267.07322021DOI Listing
July 2021

Synthesis, bioevaluation and docking studies of new imidamide derivatives as nitric oxide synthase inhibitors.

Bioorg Med Chem 2021 Jun 28;44:116294. Epub 2021 Jun 28.

Departamento de Química Farmacéutica y Orgánica, Facultad de Farmacia, Universidad de Granada, Spain. Electronic address:

In search of new Nitric Oxide Synthase (NOS) inhibitor agents, two isosteric series of derivatives with an imidamide scaffold (one of them with a hydroxyl group and the other with a carbonyl one) were synthesized and evaluated on inducible (iNOS) and neuronal (nNOS) isoforms. These compounds have been designed by combining a kynurenamine framework with an amidine moiety in order to improve selectivity for the inducible isoform. In general, the in vitro inhibitory assays exhibited better inhibition values on the iNOS isoform, being the N-(3-(2-amino-5-methoxyphenyl)-3-hydroxypropyl)-4-(trifluoromethyl)benzimidamide 4i the most active inhibitor with the highest iNOS selectivity, without inhibiting eNOS. Docking studies on the two most active compounds suggest a different binding mode on both isozymes, supporting the experimentally observed selectivity towards the inducible isoform. Physicochemical in silico studies suggest that these compounds possess good drug-likeness properties.
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http://dx.doi.org/10.1016/j.bmc.2021.116294DOI Listing
June 2021
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