Publications by authors named "Mónica Chávez-Vivas"

4 Publications

  • Page 1 of 1

Structure of Bacterial Community with Resistance to Antibiotics in Aquatic Environments. A Systematic Review.

Int J Environ Res Public Health 2021 Feb 27;18(5). Epub 2021 Feb 27.

Department of Biomedical Sciences, Faculty of Health, Campus Pampalinda, Universidad Santiago de Cali, Cali Calle 5 # 62-00 Colombia.

Aquatic environments have been affected by the increase in bacterial resistant to antibiotics. The aim of this review is to describe the studies carried out in relation to the bacterial population structure and antibiotic resistance genes in natural and artificial water systems. We performed a systematic review based on the PRISMA guideline (preferred reporting items for systematic reviews and meta-analyzes). Articles were collected from scientific databases between January 2010 and December 2020. Sixty-eight papers meeting the inclusion criteria, i.e., "reporting the water bacterial community composition", "resistance to antibiotics", and "antibiotic resistance genes (ARG)", were evaluated according to pre-defined validity criteria. The results indicate that the predominant phyla were and in natural and artificial water systems. Gram-negative bacteria of the family with resistance to antibiotics are commonly reported in drinking water and in natural water systems. The ARGs mainly reported were those that confer resistance to β-lactam antibiotics, aminoglycosides, fluoroquinolones, macrolides and tetracycline. The high influence of anthropogenic activity in the environment is evidenced. The antibiotic resistance genes that are mainly reported in the urban areas of the world are those that confer resistance to the antibiotics that are most used in clinical practice, which constitutes a problem for human and animal health.
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http://dx.doi.org/10.3390/ijerph18052348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957730PMC
February 2021

Interleukin-10 (IL-10) 1082 promoter polymorphisms and plasma IL-10 levels in patients with bacterial sepsis.

Rom J Intern Med 2021 Mar 5;59(1):50-57. Epub 2021 Mar 5.

Hospital Piloto de Popayan, Cauca, Colombia.

Interleukin-10 (IL-10) is a multifunctional cytokine which has been seen to play a relevant role in the pathogenesis of sepsis. We examined the association between a single nucleotide polymorphism (SNP) in IL-10-1082G/A in patients with sepsis in Cali city. A total of 100 patients with sepsis and 50 control subjects were enrolled in this study. Blood samples were collected from all patients in EDTA containing tubes. IL-10-1082G/A gene promoter polymorphism was analyzed by Sequence Specific Polymerase Chain Reaction (SS-PCR), while levels of serum IL-10 were measured by Enzyme Linked Immunoassay Assay (ELISA) in patients with sepsis and healthy controls. AA homozygous genotype was found more frequently in patients (32%), compared with controls (18%). AA homozygous patients showed an increased risk of developing infection by Gram-negative bacteria (OR = 2,875; 95% CI = 1.162-7.113; p = 0.020), and significantly high plasma levels of IL-10 (OR = 4.800, 95% CI 1.652-13.944; p = 0.002). AA homozygous patients high plasma IL-10 levels have greater risk of developing sepsis (63.6%; OR = 4,894; 95% CI: 1,337-17,909; p = 0.002). In this group, Afro-Colombian individuals were overrepresented among the sepsis patients with high plasma IL-10 levels (OR = 1.661; 95% CI: 1.408-1.959; p = 0.036). Our study concluded that AA genotype of IL-10-1082G/A polymorphism is a risk factor for high IL-10 production and development of sepsis by Gram negative bacteria, especially in Afro-Colombian patients.
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http://dx.doi.org/10.2478/rjim-2020-0033DOI Listing
March 2021

Characterization of multidrug-resistant strains isolated from medical intensive care units in Cali - Colombia.

Colomb Med (Cali) 2017 Dec 30;48(4):183-190. Epub 2017 Dec 30.

Grupo Microambiente Libre Departamento de Ciencias Biomédicas. Facultad de Salud. Universidad Santiago de Cali. Cali. Colombia.

Introduction: The extensive use of antibiotics has led to the emergence of multi-resistant strains in some species of the genus .

Objective: To investigate the molecular characteristics of multidrug-resistant of strains isolated from 52 patients collected between March 2009 and July 2010 in medical intensive care units in Cali - Colombia.

Methods: The susceptibility to various classes of antibiotics was determined by disc diffusion method, and the determination of the genomic species was carried out using amplified ribosomal DNA restriction analysis (ARDRA) and by sequencing of the 16s rDNA gene. Also, the genes of beta-lactamases as well as, integrases IntI1 and IntI2 were analyzed by PCR method.

Results: The phenotypic identification showed that the isolates belong mainly to All of them were multi-resistant to almost the whole antibiotics except to tigecycline and sulperazon, and they were grouped into five (I to V) different antibiotypes, being the antibiotype I the most common (50.0%). The percent of beta-lactamases detected was: blaTEM (17.3%), blaCTX-M (9.6%), blaVIM (21.2%), blaIMP (7.7%), blaOXA-58 (21.2%), and blaOXA-51 (21.2%). The phylogenetic tree analysis showed that the isolates were clustering to (74.1%), (11.1%) and (7.4 %). Besides, the integron class 1 and class 2 were detected in 23.1% and 17.3% respectively.

Conclusion: The isolates were identified to species mainly, and they were multiresistant. The resistance to beta-lactams may be by for presence of beta-lactamases in the majority of the isolates.
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http://dx.doi.org/10.25100/cm.v48i4.2858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5896725PMC
December 2017

Characterization of Staphylococcus aureus Isolates That Colonize Medical Students in a Hospital of the City of Cali, Colombia.

Int J Microbiol 2015 1;2015:358489. Epub 2015 Oct 1.

Centro de Estudios e Investigacion en Salud (CEIS), Facultad de Salud, Universidad Santiago de Cali, Hospital San Juan de Dios de Cali, Carrera 4, No. 17-67, Cali, Colombia.

Introduction. Nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) represents a risk for the spread of bacteria. This study characterized the S. aureus isolated from medical students, who were in their clinical rotation at a hospital in the city of Cali. Materials and Methods. 216 students participated in the study and 63 isolates of S. aureus were evaluated for susceptibility and PCR amplification of agr and mecA genes. The origin of MRSA isolates was established by analyzing agr polymorphisms. Results. A total of 29.2% of students were colonized by S. aureus and nasal carriage rate was 23.6% and 14.3% MRSA. Three agr groups (agr II, and agr III) were identified; the agr I group was the most common, with a 35% prevalence; this group is from community origin. Conclusion. The present study demonstrates that medical students carry S. aureus strains, with the threat of spreading them both to community and hospital environments.
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http://dx.doi.org/10.1155/2015/358489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4606190PMC
October 2015