Publications by authors named "Luqing Wei"

44 Publications

Cortical thickness and resting-state cardiac function across the lifespan: A cross-sectional pooled mega-analysis.

Psychophysiology 2020 Oct 10. Epub 2020 Oct 10.

Norwegian Centre for Mental Disorders Research (NORMENT), Institute of Clinical Medicine, University of Oslo, Oslo, Norway.

Understanding the association between autonomic nervous system [ANS] function and brain morphology across the lifespan provides important insights into neurovisceral mechanisms underlying health and disease. Resting-state ANS activity, indexed by measures of heart rate [HR] and its variability [HRV] has been associated with brain morphology, particularly cortical thickness [CT]. While findings have been mixed regarding the anatomical distribution and direction of the associations, these inconsistencies may be due to sex and age differences in HR/HRV and CT. Previous studies have been limited by small sample sizes, which impede the assessment of sex differences and aging effects on the association between ANS function and CT. To overcome these limitations, 20 groups worldwide contributed data collected under similar protocols of CT assessment and HR/HRV recording to be pooled in a mega-analysis (N = 1,218 (50.5% female), mean age 36.7 years (range: 12-87)). Findings suggest a decline in HRV as well as CT with increasing age. CT, particularly in the orbitofrontal cortex, explained additional variance in HRV, beyond the effects of aging. This pattern of results may suggest that the decline in HRV with increasing age is related to a decline in orbitofrontal CT. These effects were independent of sex and specific to HRV; with no significant association between CT and HR. Greater CT across the adult lifespan may be vital for the maintenance of healthy cardiac regulation via the ANS-or greater cardiac vagal activity as indirectly reflected in HRV may slow brain atrophy. Findings reveal an important association between CT and cardiac parasympathetic activity with implications for healthy aging and longevity that should be studied further in longitudinal research.
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http://dx.doi.org/10.1111/psyp.13688DOI Listing
October 2020

[Knockdown of Kruppel like factor 4 (KLF4) promotes RAW264.7 macrophages into M1 polarization].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2020 Sep;36(9):782-787

Characteristic Medical Center of Chinese People's Armed Police Force, Tianjin 300162, China.

Objective To investigate the effects of Kruppel like factor 4 (KLF4) gene knockdown on the polarization of RAW264.7 macrophages. Methods KLF4 knockdown lentiviral vector was constructed by RNA interfering. The lentiviral vector was transfected into RAW264.7 cells to realize stable KLF4 gene silencing in RAW264.7 cells. Interleukin-4 (IL-4) was used to stimulate macrophages in wild type group, KLF4 knockdown group and negative control group. The mRNA expression of inducible nitric oxide synthase (iNOS), IL-1β, tumor necrosis factor α (TNF-α) and Arg1, IL-10, transforming growth factor β (TGF-β) of the cells was detected by reverse transcription-PCR. Immunocytochemical staining was used to detect and localize iNOS and Arg1 protein in RAW264.7 cells. Results Levels of iNOS and IL-1β mRNA in RAW264.7 cells were significantly raised, while levels of Arg1, IL-10 and TGF-β mRNA were significantly reduced after KLF4 gene knockdown. Levels of KLF4, Arg1, IL-10 and TGF-β mRNA went up, while the relative levels of iNOS, IL-1β and TNF-α mRNA went down in wild-type RAW264.7 cells after IL-4 intervention. After shKLF4 group was intervened by IL-4, levels of iNOS, IL-1β and TNF-α mRNA in shKLF4 group (lentivirus group) were lower than those in wild-type group and higher than those in negative control group. Levels of Arg1, IL-10 and TGF-β mRNA in shKLF4 group after IL-4 treatment were higher than those in wild-type group, while Arg1 and IL-10 were lower than those in negative control group. Compared with wide-type group, the expression of iNOS protein significantly decreased, while Arg1 protein significantly increased in shKLF4 group 12 hours after IL-4 treatment. Conclusion Knockdown of KLF4 promotes the polarization of RAW264.7 macrophages into M1 as well as inhibits their polarization into M2.
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September 2020

Effects of Qizhukangxian granules on idiopathic pulmonary fibrosis: a randomized, double blind, placebo-controlled and multicenter clinical pilot trial.

J Tradit Chin Med 2020 08;40(4):674-682

Department of Hospital Management, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.

Objective: To evaluate the effects of Qizhukangxian granules (QG) on idiopathic pulmonary fibrosis (IPF).

Methods: This is a randomized, double blind, placebo-controlled and multicenter clinical pilot trial. Six medical centers in Tianjin, China, participated in the study. A total of 120 IPF patients were enrolled and randomized into two groups, with 60 patients in each group. The treatment group was treated with QG, while the control group received a Qizhukangxian placebo. The pharmacological treatment lasted for 48 weeks from the enrollment date. The indexes of patients were recorded on the admission day and at the end of the 24th and 48th weeks. Data were analyzed to study the effects of QG; forced vital capacity, change in forced vital capacity and maximal 6-min walk test (6MWT) distance were the primary endpoints. Secondary endpoints were percentage of patients with episodes of acute exacerbation of IPF, pulmonary function, changes in pulse oxygen saturation during the 6MWT, dyspnea score, St. George's respiratory questionnaire score, arterial blood gas analyses and the total Traditional Chinese Medicine symptom pattern score.

Results: After 24 weeks of treatment, QG showed greater efficacy than the placebo in certain parameters, including the dyspnea score, Traditional Chinese Medicine symptom pattern score and some indicators in the St. George's respiratory questionnaire score. Analysis of the indexes obtained from all patients at the end of the 48th week showed that the therapeutic effects in the treatment group were significantly better than those in the control group because remarkable differences were observed in most of the primary and secondary endpoints between the two groups, except for the maximal distance of the 6MWT and arterial blood gas analyses. No adverse reaction was observed in either group during the 48-week trial treatment period.

Conclusion: QG could effectively treat IPF patients by ameliorating pulmonary function, improving the quality of life and lowering the percentage of acute exacerbations.
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http://dx.doi.org/10.19852/j.cnki.jtcm.2020.04.017DOI Listing
August 2020

Structural covariance of the salience network associated with heart rate variability.

Brain Imaging Behav 2020 Aug 3. Epub 2020 Aug 3.

Key Laboratory of Cognition and Personality, Faculty of Psychology, Southwest University, Chongqing, China.

The salience network (SN) anchored by the anterior insula and cingulate is crucial for the autonomic control of body homeostasis. Resting-sate fMRI studies have linked intrinsic SN connectivity with multiple autonomic measures, but little is known about the structural organization of this system relating to cardiac vagal function. To address the above issue, the current study used covariance analysis of MRI-based gray matter volume (GMV) to map structural covariance of SN with two independent datasets, and then determined whether interregional structural connections within SN related to individual differences in vagally-mediated heart rate variability (HRV). The results showed a significant positive association between structural covariance of the SN and vagal component of HRV in two independent samples. More importantly, the conjunction and pooled data analysis revealed that structural correlation from left anterior insula to dorsal anterior cingulate cortex positively interacted with vagally-mediated HRV. The current results demonstrated a crucial role of the SN in the cortical modulation of efferent vagal activity to the heart, and provided new insight into structural neural network implicated in cardiac vagal control.
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http://dx.doi.org/10.1007/s11682-020-00298-3DOI Listing
August 2020

Effective connectivity predicts cognitive empathy in cocaine addiction: a spectral dynamic causal modeling study.

Brain Imaging Behav 2020 Jul 24. Epub 2020 Jul 24.

Ghent Experimental Psychiatry (GHEP) Lab, Ghent University, Ghent, Belgium.

Social cognition plays a crucial role in the development and treatment of cocaine dependence. However, studies investigating social cognition, such as empathy and its underlying neural basis, are lacking. To explore the neural interactions among reward and memory circuits, we applied effective connectivity analysis on resting-state fMRI data collected from cocaine-dependent subjects. The relationship between effective connectivity within these two important circuits and empathy ability - evaluated with the Interpersonal Reactivity Index (IRI) - was assessed by machine learning algorithm using multivariate regression analysis. In accordance with the neurocircuitry disruptions of cocaine addiction, the results showed that cocaine-dependent subjects relative to healthy controls had altered resting state effective connectivity between parts of the memory and reward systems. Furthermore, effective connectivity between the memory and reward system could predict the fantasy empathy (FE) subscale scores in cocaine dependence. Overall, our findings provide further evidence for the neural substrates of social cognition in cocaine-dependent patients. These new insights could be useful for the development of new treatment programs for this substance dependency disorder.
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http://dx.doi.org/10.1007/s11682-020-00354-yDOI Listing
July 2020

Resting state differences between successful and unsuccessful restrained eaters.

Brain Imaging Behav 2020 Jul 4. Epub 2020 Jul 4.

Faculty of Psychology, Southwest University, No. 2 Tiansheng Road, Chongqing, China.

Restrained eating is a popular weight loss strategy for young women that tends to have limited effectiveness over extended periods of time. Although previous studies have explored and identified possible personality and behavior differences between successful and unsuccessful restrained eaters (REs), there has been a paucity of research on neurophysiological differences.Towards addressing this gap, we assessed brain resting state (Rs) differences in groups of unsuccessful REs (N = 39) and successful REs (N = 31). In line with hypotheses, unsuccessful REs displayed reduced regional homogeneity in brain regions involved in cognitive control (inferior parietal lobe) compared to successful REs. Regions involved in conflict monitoring (anterior cingulate cortex) were also observed to be comparatively less active in the unsuccessful RE group. Finally, based on analyses of independent components and seed-based functional connectivity, regions involved in conflict monitoring and cognitive control, especially those localized within the frontoparietal network, showed weaker connectivities among unsuccessful REs compared to their successful counterparts.These results underscore specific brain Rs differences between successful REs and unsuccessful REs in regions implicated in cognitive control and conflict monitoring.
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http://dx.doi.org/10.1007/s11682-020-00300-yDOI Listing
July 2020

Efficacy, safety, and tolerability of combined pirfenidone and N-acetylcysteine therapy: a systematic review and meta-analysis.

BMC Pulm Med 2020 May 7;20(1):128. Epub 2020 May 7.

Department of Respiratory and Critical Care Medicine, Special Medical Center of Chinese People's Armed Police Forces, Tianjin, China.

Background: While antifibrotic drugs significantly decrease lung function decline in idiopathic pulmonary fibrosis (IPF), there is still an unmet need to halt disease progression. Antioxidative therapy with N-acetylcysteine (NAC) is considered a potential additional therapy that can be combined with antifibrotics in some patients in clinical practice. However, data on the efficacy, tolerability, and safety of this combination are scarce. We performed a systematic review and meta-analysis to appraise the safety, tolerability, and efficacy of the combination compared to treatment with pirfenidone alone.

Methods: We systematically reviewed all the published studies with combined pirfenidone (PFD) and NAC (PFD + NAC) treatment in IPF patients. The primary outcomes referred to decline in pulmonary function tests (PFTs) and the rates of IPF patients with side effects.

Results: In the meta-analysis, 6 studies with 319 total IPF patients were included. The PFD + NAC group was comparable to the PFD alone group in terms of the predicted forced vital capacity (FVC%) and predicted diffusion capacity for carbon monoxide (DLco%) from treatment start to week 24. Side effects and treatment discontinuation rates were also comparable in both groups.

Conclusion: This systematic review and meta-analysis suggests that combination with NAC does not alter the efficacy, safety, or tolerability of PFD in comparison to PFD alone in IPF patients.
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http://dx.doi.org/10.1186/s12890-020-1121-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7204217PMC
May 2020

A multicenter RCT of noninvasive ventilation in pneumonia-induced early mild acute respiratory distress syndrome.

Crit Care 2019 Sep 4;23(1):300. Epub 2019 Sep 4.

Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Rationale: Our pilot study suggested that noninvasive ventilation (NIV) reduced the need for intubation compared with conventional administration of oxygen on patients with "early" stage of mild acute respiratory distress syndrome (ARDS, PaO/FIO between 200 and 300).

Objectives: To evaluate whether early NIV can reduce the need for invasive ventilation in patients with pneumonia-induced early mild ARDS.

Methods: Prospective, multicenter, randomized controlled trial (RCT) of NIV compared with conventional administration of oxygen through a Venturi mask. Primary outcome included the numbers of patients who met the intubation criteria.

Results: Two hundred subjects were randomized to NIV (n = 102) or control (n = 98) groups from 21 centers. Baseline characteristics were similar in the two groups. In the NIV group, PaO/FIO became significantly higher than in the control group at 2 h after randomization and remained stable for the first 72 h. NIV did not decrease the proportion of patients requiring intubation than in the control group (11/102 vs. 9/98, 10.8% vs. 9.2%, p = 0.706). The ICU mortality was similar in the two groups (7/102 vs. 7/98, 4.9% vs. 3.1%, p = 0.721). Multivariate analysis showed minute ventilation greater than 11 L/min at 48 h was the independent risk factor for NIV failure (OR, 1.176 [95% CI, 1.005-1.379], p = 0.043).

Conclusions: Treatment with NIV did not reduce the need for intubation among patients with pneumonia-induced early mild ARDS, despite the improved PaO/FIO observed with NIV compared with standard oxygen therapy. High minute ventilation may predict NIV failure.

Trial Registration: NCT01581229 . Registered 19 April 2012.
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http://dx.doi.org/10.1186/s13054-019-2575-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727327PMC
September 2019

The Neural Representation of Relational- and Collective-Self: Two Forms of Collectivism.

Front Psychol 2018 19;9:2624. Epub 2018 Dec 19.

Faculty of Psychology, Southwest University, Chongqing, China.

The collectivism can be divided into two forms: relational collectivism and group collectivism. According to the cognitive representation of self, relational collectivism emphasizes the relational-self and group collectivism privileges the collective-self. However, it remains uncertain whether there is a difference between relational-self and collective-self under Chinese collectivism cultural. To address the above issue, the present study examined the neural representation of relational-self and collective-self during trait judgment tasks using functional magnetic resonance imaging (fMRI). The results showed that relational-self-reference compared with collective-self-reference generated stronger medial prefrontal cortex (MPFC) activity, indicating relational-self was more closeness and important in the self-concept than collective-self under East Asian cultural background. Relational-self and collective-self are unequally represented in the MPFC, providing direct neural evidence that the collectivism in China can be divided into relational collectivism and group collectivism.
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http://dx.doi.org/10.3389/fpsyg.2018.02624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312116PMC
December 2018

Grey Matter Volumes in the Executive Attention System Predict Individual Differences in Effortful Control in Young Adults.

Brain Topogr 2019 01 10;32(1):111-117. Epub 2018 Sep 10.

Key Laboratory of Cognition and Personality, Faculty of Psychology, Southwest University, Chongqing, China.

Effortful control (EC), considered as one component of temperament, describes an individual's capacity for self-regulation. Previous neuroimaging studies have provided convergent evidence that individual differences in EC are determined by the functioning of neural systems subserving executive attention, primarily comprising the anterior cingulate cortex (ACC) and the lateral prefrontal cortex (PFC). Notwithstanding, as previous neuroimaging findings highlighted the structural neural bases of EC in adolescence, during which the PFC is prominently remodeled, the underlying neuroanatomical substrates of EC remain uncertain in young adults. In this study, we included 246 healthy young adults and used voxel-based morphometry analysis to investigate the relationship between EC and grey matter (GM) volumes. Additionally, permutation testing and cross-validation were applied to determine whether GM volumes in the detected regions could predict individual differences in EC. Our results revealed that EC was associated with GM volumes in the dorsal anterior cingulate cortex (dACC) and the pre-supplementary motor area (pre-SMA), demonstrating that these two regions may play a crucial role in EC. Furthermore, the identified regional GM volumes reliably contribute to the prediction of EC confirmed by cross-validation. Overall, these findings provide further evidence for the involvement of the executive attention system in EC, and shed more light on the neuroanatomical substrates of EC in young adulthood.
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http://dx.doi.org/10.1007/s10548-018-0676-1DOI Listing
January 2019

Synthesis and discovery of a drug candidate for treatment of idiopathic pulmonary fibrosis through inhibition of TGF-β1 pathway.

Eur J Med Chem 2018 Sep 3;157:229-247. Epub 2018 Aug 3.

College of Pharmacy, The State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300350, People's Republic of China. Electronic address:

In this study, anti-IPF lead compounds 42 and 44, derived from natural sesquiterpene lactones Isoalantolactone and alantolactone, were discovered by screening from a high-throughput TGF-β1 reporter luciferase assay. Notably, they could reduce the myofibroblast activation and extracellular matrix deposition both in vitro and in vivo. Additionally, compounds 42 and 44 could significantly attenuate bleomycin-induced pulmonary fibrosis in mice. Further validation of pharmacokinetics study and toxicity evaluation indicated that compound 44 might be a promising anti-IPF drug candidate.
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http://dx.doi.org/10.1016/j.ejmech.2018.07.074DOI Listing
September 2018

Structural Covariance of the Prefrontal-Amygdala Pathways Associated with Heart Rate Variability.

Front Hum Neurosci 2018 23;12. Epub 2018 Jan 23.

Key Laboratory of Cognition and Personality, Faculty of Psychology, Southwest University, Chongqing, China.

The neurovisceral integration model has shown a key role of the amygdala in neural circuits underlying heart rate variability (HRV) modulation, and suggested that reciprocal connections from amygdala to brain regions centered on the central autonomic network (CAN) are associated with HRV. To provide neuroanatomical evidence for these theoretical perspectives, the current study used covariance analysis of MRI-based gray matter volume (GMV) to map structural covariance network of the amygdala, and then determined whether the interregional structural correlations related to individual differences in HRV. The results showed that covariance patterns of the amygdala encompassed large portions of cortical (e.g., prefrontal, cingulate, and insula) and subcortical (e.g., striatum, hippocampus, and midbrain) regions, lending evidence from structural covariance analysis to the notion that the amygdala was a pivotal node in neural pathways for HRV modulation. Importantly, participants with higher resting HRV showed increased covariance of amygdala to dorsal medial prefrontal cortex and anterior cingulate cortex (dmPFC/dACC) extending into adjacent medial motor regions [i.e., pre-supplementary motor area (pre-SMA)/SMA], demonstrating structural covariance of the prefrontal-amygdala pathways implicated in HRV, and also implying that resting HRV may reflect the function of neural circuits underlying cognitive regulation of emotion as well as facilitation of adaptive behaviors to emotion. Our results, thus, provide anatomical substrates for the neurovisceral integration model that resting HRV may index an integrative neural network which effectively organizes emotional, cognitive, physiological and behavioral responses in the service of goal-directed behavior and adaptability.
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http://dx.doi.org/10.3389/fnhum.2018.00002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5838315PMC
January 2018

Abnormal ventral tegmental area-anterior cingulate cortex connectivity in Parkinson's disease with depression.

Behav Brain Res 2018 07 9;347:132-139. Epub 2018 Mar 9.

Key Laboratory of Personality and Cognition, Faculty of Psychology, Southwest University, Chongqing 400715, PR China. Electronic address:

Neuropathology suggests that Parkinson's disease (PD) with depression may involve a progressive degeneration of the nigrostriatal and mesocorticolimbic dopaminergic systems. Previous positron emission tomography (PET) and single-photon emission computed tomography (SPECT) studies have shown that dopamine changes in individual brain regions constituting the nigrostriatal and mesocorticolimbic circuits are associated with depression in PD. However, few studies have been conducted on the circuit-level alterations in this disease. The present study used resting-state fMRI and seed-based functional connectivity of putative dopaminergic midbrain regions (i.e., substantia nigra (SN) and ventral tegmental area (VTA)) to investigate the circuit-related abnormalities in PD with depression. The results showed that depressed PD (DPD) patients relative to healthy controls (HC) and non-depressed PD (NDPD) patients had increased functional connectivity between VTA and anterior cingulate cortex (ACC), demonstrating that dysfunctional mesocorticolimbic dopaminergic neurotransmission may be associated with depression in PD. Compared with HC, DPD and NDPD patients showed increased functional connectivity from SN to sensorimotor cortex, validating that alterations in the nigrostriatal circuitry could be responsible for cardinal motor features in PD. In addition, aberrant connectivity between VTA and ACC was correlated with the severity of depression in PD patients, further supporting that abnormal mesocorticolimbic system may account for depressive symptoms in PD. These results have provided potential circuit-level biomarkers of depression in PD, and suggested that resting state functional connectivity of midbrain dopaminergic nuclei may be useful for understanding the underlying pathology in PD with depression.
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http://dx.doi.org/10.1016/j.bbr.2018.03.011DOI Listing
July 2018

Heart rate variability associated with grey matter volumes in striatal and limbic structures of the central autonomic network.

Brain Res 2018 02 24;1681:14-20. Epub 2017 Dec 24.

Key laboratory of Cognition and Personality, Faculty of Psychology, Southwest University, Chongqing 400715, PR China. Electronic address:

Previous neuroimaging studies have highlighted the functional neural correlates of cardiac vagal activity, providing convergent evidence that the cardiac vagal function is controlled by a number of brain regions in the central autonomic network (CAN). However, it remains largely unknown whether the underlying anatomical basis of those identified regions are associated with individual difference in vagal function. To address the above issue, this study used a large sample of healthy subjects (n = 185) and voxel-based morphometry (VBM) analysis to verify brain morphometry associated with vagal control and the associations varied as a function of gender and age. Our results showed that high frequency component of heart rate variability (HF-HRV) was negatively correlated with grey matter volumes in the right putamen, caudate, amygdala, insula, superior temporal gyrus, temporal pole, and parahippocampal gyrus, demonstrating brain morphological variation in the right-sided striatal and limbic structures of the CAN associated with individual difference in cardiac vagal function. Additionally, gender and age effects on the relationship between cardiac vagal control and brain morphometry were not significant in the current dataset. These findings underscore the importance of striatal and limbic structures in parasympathetic control, and shed light on the underlying anatomical substrates of cardiac vagal activity.
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http://dx.doi.org/10.1016/j.brainres.2017.12.024DOI Listing
February 2018

Paeoniflorin inhibits PDGF‑BB‑induced human airway smooth muscle cell growth and migration.

Mol Med Rep 2018 Feb 29;17(2):2660-2664. Epub 2017 Nov 29.

Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Logistics University of Chinese People's Armed Police Force, Tianjin 300162, P.R. China.

Abnormal proliferation and migration of airway smooth muscle cells (ASMCs) is important in the progression of asthma. Paeoniflorin (PF), one of the major active ingredients of Paeonia lactiflora, has been reported to exhibit anti‑asthmatic effects. However, the effects of PF in the regulation of platelet‑derived growth factor (PDGF)‑BB‑induced ASMC proliferation and migration remain unknown. The present study was designed to investigate the effects of PF on human ASMCs and the underlying mechanism. The results demonstrated that PF treatment significantly reduced the numbers of live ASMC cells and their PDGF‑BB‑induced migration. PF treatment also suppressed PDGF‑BB‑induced α‑smooth muscle actin expression in ASMCs. Furthermore, pretreatment with PF reduced PDGF‑BB‑induced phosphorylation of phosphoinositide 3‑kinase (PI3K) and AKT serine/threonine kinase 1 (Akt) in ASMCs. In conclusion, the present study demonstrated for the first time that PF inhibited ASMC growth and migration induced by PDGF‑BB, and that this effect may be partly due to inhibition of the PI3K/Akt signaling pathway. The results provide novel information regarding the role of PF as a potential therapeutic agent for the treatment of asthma.
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http://dx.doi.org/10.3892/mmr.2017.8180DOI Listing
February 2018

Aberrant Intra- and Internetwork Functional Connectivity in Depressed Parkinson's Disease.

Sci Rep 2017 05 31;7(1):2568. Epub 2017 May 31.

Key laboratory of Personality and Cognition, Faculty of Psychology, Southwest University, Chongqing, 400715, P.R. China.

Much is known concerning the underlying mechanisms of Parkinson's disease (PD) with depression, but our understanding of this disease at the neural-system level remains incomplete. This study used resting-state functional MRI (rs-fMRI) and independent component analysis (ICA) to investigate intrinsic functional connectivity (FC) within and between large-scale neural networks in 20 depressed PD (dPD) patients, 35 non-depressed PD (ndPD) patients, and 34 healthy controls (HC). To alleviate the influence caused by ICA model order selection, this work reported results from analyses at 2 levels (low and high model order). Within these two analyses, similar results were obtained: 1) dPD and ndPD patients relative to HC had reduced FC in basal ganglia network (BGN); 2) dPD compared with ndPD patients exhibited increased FC in left frontoparietal network (LFPN) and salience network (SN), and decreased FC in default-mode network (DMN); 3) dPD patients compared to HC showed increased FC between DMN and LFPN. Additionally, connectivity anomalies in the DMN, LFPN and SN correlated with the depression severity in patients with PD. Our findings confirm the involvement of BGN, DMN, LFPN and SN in depression in PD, facilitating the development of more detailed and integrative neural models of PD with depression.
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http://dx.doi.org/10.1038/s41598-017-02127-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5451438PMC
May 2017

Frequency of Spontaneous BOLD Signal Differences between Moderate and Late Preterm Newborns and Term Newborns.

Neurotox Res 2016 10 17;30(3):539-51. Epub 2016 Jun 17.

Department of Pediatrics, Daping Hospital, Third Military Medical University, 400042, Chongqing, China.

Little is known about the frequency features of spontaneous neural activity in the brains of moderate and late preterm (MLPT) newborns. We used resting-state functional magnetic resonance imaging (rs-fMRI) and the amplitude of low-frequency fluctuation (ALFF) method to investigate the frequency properties of spontaneous blood oxygen level-dependent (BOLD) signals in 26 MLPT and 35 term newborns. Two frequency bands, slow-4 (0.027-0.073 Hz) and slow-5 (0.01-0.027 Hz), were analyzed. Our results showed widespread differences in ALFF between the two bands; differences occurred mainly in the primary sensory and motor cortices and to a lesser extent in association cortices and subcortical areas. Compared with term newborns, MLPT newborns showed significantly altered neural activity predominantly in the primary sensory and motor cortices and in the posterior cingulate gyrus/precuneus. In addition, a significant interaction between frequency bands and groups was observed in the primary somatosensory cortex. Intriguingly, these primary sensory and motor regions have been proven to be the major cortical hubs during the neonatal period. Our results revealed the frequency of spontaneous BOLD signal differences between MLPT and term newborns, which contribute to the understanding of regional development of spontaneous brain rhythms of MLPT newborns.
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http://dx.doi.org/10.1007/s12640-016-9642-4DOI Listing
October 2016

[The efficiency of macrophage extracellular trap formation induced by different inducers in vitro].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2016 Apr;32(4):466-9

Department of Respiratory and Critical Care Medicine, Pingjin Hospital, Logistics University of the Chinese People's Armed Police Forces, Tianjin 300162, China. *Corresponding authors,

Objective: To compare the different methods of inducing the formation of macrophage extracellular trap (MET) in vitro.

Methods: MET release was initiated by culturing RAW264.7 cells with 0.5, 1, 5, 10 μg/mL lipopolysaccharide (LPS), or 10, 25, 50, 80 μmol/L phorbolmyristate acetate (PMA), or 50, 100, 150 μg/mL silicon dioxide (SiO2). Three and 6 hours later, MET were validated by immunofluorescence staining, followed by immunofluorescence-based semi-quantitative analysis.

Results: Immunofluorescence staining showed that the network structures were mainly composed of DNA and histones. RAW264.7 cells treated with 1 μg/mL LPS for 6 hours produced the highest percent of MET [(37.04±10.02)%], which was statistically higher compared with control group [(7.90±2.71)%]. RAW264.7 cells treated with 80 μmol/L PMA for 6 hours also produced the higher percent of MET [(22.40±1.83)%] compared with control group [(10.11±1.13)%]. However, there was no significantly increased MET formation in cells treated with SiO2 compared with control group.

Conclusion: LPS and PMA can induce MET formation in vitro, while SiO2 was not efficient inducer.
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April 2016

[A comparison study of diagnostic value between the old and revised guidelines in patients with idiopathic pulmonary fibrosis].

Zhonghua Nei Ke Za Zhi 2016 Feb;55(2):131-4

Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Logistics University of Chinese People's Armed Police Force, Tianjin 300162, China.

Objective: To compare the strengths and limitations of the old and revised guidelines for the diagnosis in patients with idiopathic pulmonary fibrosis(IPF).

Methods: Patients who were admitted and diagnosed as interstitial lung diseases (ILDs) in our hospital from 2009 to 2014 were enrolled in our study.Eachpatient was reevaluated respectively according to the old and revised guidelines of IPF.

Results: A total of 553 cases were initially reviewed, among whom 355 cases were excluded for pulmonary fibrosis secondary to definite underlying diseases, 28 excluded due to high resolution computed tomography(HRCT) not done, 26 excluded because serum immunology examination was not available.The remaining 144 cases were finally enrolled in this study including 92 males and 52 females with median age 21-92 (68 ± 11) years old. Twenty five patients (17.4%, 25/144) met the diagnostic criteria of IPF by the old guideline.While by the revised guideline, 53 patients (36.8%, 53/144) were diagnosed as classical IPF, 29 patients(20.1%, 29/144) as probable cases, another 69 non-IPF patients accounting for 43.1% (62/144). The result revealed that there's a significant difference between the two guidelines in the diagnosis of IPF.

Conclusions: The revised guideline favors an early diagnosis of IPF and simplifies the diagnostic process.However the possibility of over diagnosis or missed diagnosis by the revised guideline does exist.On the other hand, despite of the delayed diagnosis by the old guideline, it may reduce the misdiagnosis of IPF in some circumstance.
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http://dx.doi.org/10.3760/cma.j.issn.0578-1426.2016.02.013DOI Listing
February 2016

[Analysis of prognostic value of initial serum paraquat concentration in patients with paraquat poisoning].

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2015 Sep;33(9):697-700

Department of Respiratory and Critical Care Medicine Pingjin Hospital, Affiliated Hospital of Armed Police Logistics College, Tianjin 300162, China; E-mail:

Objective: To investigate the prognostic values of initial serum paraquat (PQ) concentration and time lag after PQ ingestion in patients with PQ poisoning.

Methods: A retrospective analysis was performed on 189 patients who ingested PQ between 2.5 and 48 h before admission. The values of initial serum PQ concentration and time lag after PQ ingestion for the prognosis after poisoning were analyzed by the receiver operating characteristic (ROC) curve and multiple logistic regression analysis.

Results: The serum PQ concentration of non-survivors was significantly higher than that of survivors (P<0.01) , and the time lag after PQ ingestion of non-survivors was significantly longer than that of survivors (P<0.01). The ROC curve analysis showed that the area under the ROC curve (AUC) showed no significant difference between initial serum PQ concentration and serum PQ concentration-time data when the time lag after PQ ingestion was between 2.5 and 48 h (z=0.563, P=0.574) ; the AUC showed a significant difference between initial serum PQ concentration and serum PQ concentration-time data when the time lag after PQ ingestion was between 2.5 and 6 h (z=2.199, P=0.034) and between 6 and 48 h (z=2.525, P=0.012).

Conclusion: Initial serum PQ concentration has a better predictive power than serum PQ concentration-time data in evaluating the prognosis of patients when the time lag after PQ ingestion is within 6 h. However, serum PQ concentration-time data has a better predictive power than PQ concentration alone in evaluating the prognosis of patients when the time lag after PQ ingestion is between 6 and 48 h.
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September 2015

[Outbreak of six cases of nosocomial Legionella pneumophila pneumonia].

Zhonghua Jie He He Hu Xi Za Zhi 2015 Apr;38(4):294-7

Email:

Objectives: To describe the clinical characteristics, diagnosis, treatment and the causes of outbreak of nosocomial pneumonia due to Legionella pneumophila.

Methods: The medical records of 6 cases of nosocomial Legionella pneumophila pneumonia were retrospectively reviewed, and the clinical data of clinical presentation, treatment, and etiologic diagnosis were analyzed.

Results: The 6 patients were health care providers of the Department of Respiratory and Critical Care Medicine of the Affiliated Hospital of Logistics University of PAPF. There were 5 female and 1 male patients, aged 23 to 27 years. The diagnosis of Legionella pneumonia was made based on a positive Legionella urinary I antigen test. In all the 6 cases, the disease was attributable to inhaling contaminated aerosols produced by the air conditioning system in our hospital. All the 6 patients presented with fever of 37.5-39 °C, and productive cough. One patient had anorexia, dyspnoea, and pleuritic chest pain, while headache was reported in 3 patients. Physical examinations revealed lung rales in 3 patients. Single or multiple patchy infiltrates were found on Chest CT in all patients. However, the typical extrapulmonary manifestations of Legionnaires' disease, including relative bradycardia, hyponatremia, hypophosphatemia and diarrhea, were not present in the 6 patients. Neither neurological abnormalities nor renal involvement were observed.

Conclusions: Our data demonstrated that Legionella pneumonia may be mild with atypical laboratory findings and clinical manifestations, with fever, cough, chest tightness, headache, etc. The radiologic manifestations of Legionella pneumonia were single or multiple patchy infiltrates, similar to those of viral pneumonia, and therefore their differential diagnosis was needed. Legionella pneumophila can outbreak through the respiratory tract, in public places with central air-conditioning system, hot water piping systems and hospitals, and therefore microbiological monitoring, cleaning and disinfection of the water system was required for prevention of the outbreaks of Legionnaires' disease.
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April 2015

Distinct patterns of spontaneous brain activity between children and adults with anisometropic amblyopia: a resting-state fMRI study.

Graefes Arch Clin Exp Ophthalmol 2016 Mar 6;254(3):569-76. Epub 2015 Aug 6.

Department of Radiology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.

Purpose: To detect the altered spontaneous brain activity patterns in children and adults with anisometropic amblyopia using resting-state functional magnetic resonance imaging (rs-fMRI) technique combined with the amplitude of low-frequency fluctuation (ALFF) method.

Methods: Thirty-two monocular anisometropic amblyopia and 34 normal-sight controls were divided into child group and adult group. Rs-fMRI was performed in all participants and analysis of ALFF value within the whole brain was conducted in each subject. ALFF value differences between the patients and controls in the two groups were compared via an independent two-sample t test.

Results: The amblyopic children mainly exhibited increased ALFF in part of the bilateral calcarine (BA17), the left middle occipital gyrus (BA18/19), and the left postcentral gyrus (BA2). By contrast, the amblyopic adults showed decreased ALFF in the bilateral precuneus cortex (part of BA7), and the standardized ALFF value of bilateral precuneus were correlated with the amount of anisometropia of the amblyopic adults.

Conclusions: Rs-fMRI is an effective noninvasive technique for exploring brain activity of the anisometropic amblyopia. Our findings demonstrated that brain activity changed both in amblyopic children and adults under the resting state, and revealed the differences in spontaneous activity patterns between the amblyopic children and adults.
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http://dx.doi.org/10.1007/s00417-015-3117-9DOI Listing
March 2016

[The value of assessment of area of ground glass opacity in lungs cast by high-resolution computed tomography on the prognosis of patients with acute paraquat intoxication].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2015 Apr;27(4):270-3

Department of Magnetic Resonance Imaging, Affiliated Hospital of the Medical College of Chinese People's Armed Police Forces, Tianjin 300162, China. Corresponding author: Wei Luqing, Email:

Objective: To assess the value of the area of ground glass opacities (GGOs) in lungs displayed by high-resolution computed tomography (HRCT) in paraquat (PQ) poisoned patients in evaluating prognosis.

Methods: Clinical and imaging data of 137 patients with acute PQ poisoning admitted to Affiliated Hospital of the Medical College of Chinese People's Armed Police Forces from January 2012 to August 2014 were analyzed retrospectively. The plasma concentration of PQ on admission and the area of GGOs were compared between two groups. The lung HRCT within 10 days of poisoning was performed every 3 days, and the areas of GGOs were evaluated on five levels, including aortic arch, aortic pulmonary window, left upper lobe bronchial, right inferior pulmonary vein, and left diaphragmatic dome. Receiver operating characteristic curve (ROC) was plotted to evaluate the value of all the parameters for prognosis.

Results: Among 137 patients, 45 died within 28 days after poisoning, with the mortality rate of 32.85%. The plasma PQ level in the non-survivors was significantly higher than that in the survivors (mg/L: 7.06 ± 0.67 vs. 3.51 ± 0.34, t = 5.280, P = 0.000). The areas of GGOs at three time points in the non-survivors were significantly higher than those in the survivors [ 1-3 days: (32.0 ± 5.0)% vs. (2.5 ± 0.4)%, t = 7.860, P = 0.000; 4-6 days: (45.5 ± 5.7)% vs. (2.8 ± 0.5)%, t = 12.420, P = 0.000; 7-10 days: (68.0 ± 4.8)% vs. (3.0 ± 0.6)%, t = 23.950, P = 0.00 ]. ROC analysis demonstrated that the area under the ROC curve (AUC) of GGOs in 7-10 days was 1.000, which could be used to determine the prognosis, but it was too late for the treatment. The AUC of GGOs in 4-6 days was 0.979, with the threshold of > 12.0%, the specificity of 96.15%, the sensitivity of 85.19%, the positive predictive value of 88.46%, and the negative predictive value of 94.94%, which presented good effect in predicting prognosis in the early stage of acute PQ intoxication. But plasma PQ concentration was relatively poor for determining prognosis, AUC was 0.821, with the threshold of > 1.95 mg/L, the specificity of 34.52%, the sensitivity of 88.64%, the positive predictive value of 41.49%, and the negative predictive value of 85.29%.

Conclusions: The area of GGOs displayed by HRCT can be used to evaluate the fully developed acute PQ lung injury, and it is superior to plasma PQ concentration. The area of GGOs displayed by HRCT 4-6 days after intoxication can be used for the evaluation of PQ induced pulmonary injury in the early stage and the evaluation of clinical prognosis.
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http://dx.doi.org/10.3760/cma.j.issn.2095-4352.2015.04.009DOI Listing
April 2015

Relevance analysis of clinical and lung function parameters changing and prognosis of idiopathic pulmonary fibrosis.

Int J Clin Exp Med 2014 15;7(12):4759-69. Epub 2014 Dec 15.

Institute of Respiratory Diseases, The First Affiliated Hospital, China Medical University Shenyang, People's Republic of China.

Objective: Idiopathic pulmonary fibrosis (IPF) is defined as a specific form of chronic, progressive fibrosing interstitial pneumonia with unknown cause. We analyzed the changed rate of pulmonary function and arterial blood gas in IPF patients, and evaluated their influence of changed rate to IPF prognosis.

Methods: 81 patients with IPF were recruited successfully, they were followed-up at 6 and 12 months. Dyspnea score and respiratory assessment parameters including FVC, FEV1, TLC, SaO2, PA-aO2, and DLCO were evaluated at their 6 and 12 months follow-up. The changed value and changed rate of above parameters were calculated, and their treatment effects were divided into 3 subgroup: improved, stable and deteriorated group. Statistical analysis was performed between groups for survival and hazards regression analysis.

Results: 55 of 81 patients were follow-up at 12 months. Dyspnea score and its changed rate, the changed value of FEV1%, FVC%, TLC%, DLCO%, and PaO2, SaO2, PA-aO2 were prognosis effect factors in IPF patients in 6 and 12 months group. The survival analysis of dyspnea scores, FVC%, TLC%, DLCO%, PaO2, SaO2 and PA-aO2 at K-M were all statistical significant (P < 0.05) in improved, stable and deteriorated group.

Conclusion: FVC% changed rate, dyspnea score changed rate and PaO2 changed rate were IPF patient prognosis associated factors in 6 months group; and FVC% changed rate, DLCO% changed rate and TLC% changed rate were prognosis associated factors for IPF patient in 12 months group.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307419PMC
February 2015

Decreased interhemispheric functional connectivity in subtypes of Parkinson's disease.

J Neurol 2015 Mar 11;262(3):760-7. Epub 2015 Jan 11.

Department of Radiology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, People's Republic of China.

Parkinson's disease (PD) can be classified into tremor-dominant (TD) subtype and akinetic-rigid (AR) subtype, which exhibit different clinical courses and prognoses. However, the neural mechanisms underlying different subtypes of PD are not well understood. Using voxel-mirrored homotopic connectivity (VMHC), we examined the homotopic resting-state functional connectivity patterns in akinetic-rigid PD (AR-PD) and tremor-dominant PD (TD-PD) to study the neural basis for these disparate manifestations of PD. Twenty-one TD-PD patients, 29 AR-PD patients and 26 normal control subjects participated in this study. Resting-state fMRI data were analyzed using VMHC. Correlations between VMHC values and each clinical characteristic were also calculated. Compared with normal control subjects and subjects with AR-PD, subjects with TD-PD exhibited significantly lower VMHC values in the posterior lobe of the cerebellum. Moreover, tremor scores and VMHC values for the cerebellum were found to be significantly negatively correlated in TD-PD patients. By contrast, subjects with AR-PD exhibited lower VMHC values in the precentral gyrus compared with normal control subjects. These findings suggest that functional coordination between homotopic brain regions is impaired in AR-PD and TD-PD patients. This study provides evidence of both cerebellum-related connectivity deficits in TD-PD. The finding that VMHC values and tremor scores were significantly correlated suggests that VMHC measurements may be of potential clinical relevance in TD-PD.
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http://dx.doi.org/10.1007/s00415-014-7627-xDOI Listing
March 2015

Akinetic-rigid and tremor-dominant Parkinson's disease patients show different patterns of intrinsic brain activity.

Parkinsonism Relat Disord 2015 Jan 27;21(1):23-30. Epub 2014 Oct 27.

Department of Radiology, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China. Electronic address:

Background: Parkinson's disease (PD) is a surprisingly heterogeneous neurodegenerative disorder. It is well established that different subtypes of PD present with different clinical courses and prognoses. However, the neural mechanism underlying these disparate presentations is uncertain.

Methods: Here we used resting-state fMRI (rs-fMRI) and the regional homogeneity (ReHo) method to determine neural activity patterns in the two main clinical subgroups of PD (akinetic-rigid and tremor-dominant).

Results: Compared with healthy controls, akinetic-rigid (AR) subjects had increased ReHo mainly in right amygdala, left putamen, bilateral angular gyrus, bilateral medial prefrontal cortex (MPFC), and decreased ReHo in left post cingulate gyrus/precuneus (PCC/PCu) and bilateral thalamus. In contrast, tremor-dominant (TD) patients showed higher ReHo mostly in bilateral angular gyrus, left PCC, cerebellum_crus1, and cerebellum_6, while ReHo was decreased in right putamen, primary sensory cortex (S1), vermis_3, and cerebellum_4_5. These results indicate that AR and TD subgroups both represent altered spontaneous neural activity in default-mode regions and striatum, and AR subjects exhibit more changed neural activity in the mesolimbic cortex (amygdala) but TD in the cerebellar regions. Of note, direct comparison of the two subgroups revealed a distinct ReHo pattern primarily located in the striatal-thalamo-cortical (STC) and cerebello-thalamo-cortical (CTC) loops.

Conclusion: Overall, our findings highlight the involvement of default mode network (DMN) and STC circuit both in AR and TD subtypes, but also underscore the importance of integrating mesolimbic-striatal and CTC loops in understanding neural systems of akinesia and rigidity, as well as resting tremor in PD. This study provides improved understanding of the pathophysiological models of different subtypes of PD.
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http://dx.doi.org/10.1016/j.parkreldis.2014.10.017DOI Listing
January 2015

Reduced topological efficiency in cortical-basal Ganglia motor network of Parkinson's disease: a resting state fMRI study.

PLoS One 2014 3;9(10):e108124. Epub 2014 Oct 3.

Department of Radiology, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China.

Parkinson's disease (PD) is mainly characterized by dopamine depletion of the cortico-basal ganglia (CBG) motor circuit. Given that dopamine dysfunction could affect functional brain network efficiency, the present study utilized resting-state fMRI (rs-fMRI) and graph theoretical approach to investigate the topological efficiency changes of the CBG motor network in patients with PD during a relatively hypodopaminergic state (12 hours after a last dose of dopamimetic treatment). We found that PD compared with controls had remarkable decreased efficiency in the CBG motor network, with the most pronounced changes observed in rostral supplementary motor area (pre-SMA), caudal SMA (SMA-proper), primary motor cortex (M1), primary somatosensory cortex (S1), thalamus (THA), globus pallidus (GP), and putamen (PUT). Furthermore, reduced efficiency in pre-SMA, M1, THA and GP was significantly correlated with Unified Parkinson's Disease Rating Scale (UPDRS) motor scores in PD patients. Together, our results demonstrate that individuals with PD appear to be less effective at information transfer within the CBG motor pathway, which provides a novel perspective on neurobiological explanation for the motor symptoms in patients. These findings are in line with the pathophysiology of PD, suggesting that network efficiency metrics may be used to identify and track the pathology of PD.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0108124PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184784PMC
July 2015