Publications by authors named "Lulu An"

11 Publications

  • Page 1 of 1

Cardiac Dysfunction in a Mouse Vascular Dementia Model of Bilateral Common Carotid Artery Stenosis.

Front Cardiovasc Med 2021 11;8:681572. Epub 2021 Jun 11.

Department of Neurology, Henry Ford Hospital, Detroit, MI, United States.

Cardiac function is associated with cognitive function. Previously, we found that stroke and traumatic brain injury evoke cardiac dysfunction in mice. In this study, we investigate whether bilateral common carotid artery stenosis (BCAS), a model that induces vascular dementia (VaD) in mice, induces cardiac dysfunction. Late-adult (6-8 months) C57BL/6J mice were subjected to sham surgery ( = 6) or BCAS ( = 8). BCAS was performed by applying microcoils (0.16 mm internal diameter) around both common carotid arteries. Cerebral blood flow and cognitive function tests were performed 21-28 days post-BCAS. Echocardiography was conducted in conscious mice 29 days after BCAS. Mice were sacrificed 30 days after BCAS. Heart tissues were isolated for immunohistochemical evaluation and real-time PCR assay. Compared to sham mice, BCAS in mice significantly induced cerebral hypoperfusion and cognitive dysfunction, increased cardiac hypertrophy, as indicated by the increased heart weight and the ratio of heart weight/body weight, and induced cardiac dysfunction and left ventricular (LV) enlargement, indicated by a decreased LV ejection fraction (LVEF) and LV fractional shortening (LVFS), increased LV dimension (LVD), and increased LV mass. Cognitive deficits significantly correlated with cardiac deficits. BCAS mice also exhibited significantly increased cardiac fibrosis, increased oxidative stress, as indicated by 4-hydroxynonenal and NADPH oxidase-2, increased leukocyte and macrophage infiltration into the heart, and increased cardiac interleukin-6 and thrombin gene expression. BCAS in mice without primary cardiac disease provokes cardiac dysfunction, which, in part, may be mediated by increased inflammation and oxidative stress.
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http://dx.doi.org/10.3389/fcvm.2021.681572DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225957PMC
June 2021

Metoprolol attenuates intracerebral hemorrhage-induced cardiac damage by suppression of sympathetic overactivity in mice.

Auton Neurosci 2021 Sep 5;234:102832. Epub 2021 Jun 5.

Department of Neurology, Tianjin Medical University General Hospital, Tianjin Neurological Institute, Key Laboratory of Post-Neurotrauma, Neurorepair, and Regeneration in Central Nervous System, Ministry of Education and Tianjin City, Tianjin 300052, China. Electronic address:

The high rates of mortality and disability resulting from intracerebral hemorrhage (ICH) are closely related to subsequent cardiac complications. The mechanisms underlying ICH-induced cardiac dysfunction are not fully understood. In this study, we investigated the role of sympathetic overactivity in mediating cardiac dysfunction post ICH in mice. Collagenase-injection ICH model was established in adult male C57BL/6J mice. Neurological function was subsequently evaluated at multiple time points after ICH and cardiac function was measured by echocardiography on 3 and 14 days after ICH. Plasma adrenaline, noradrenaline, cortisol and heart β1 adrenergic receptor (β1-AR) levels were assessed to evaluate sympathetic activity. Picro Sirius Red (PSR) staining was performed to evaluate cardiomyocyte hypertrophy and interstitial fibrosis. Monocyte chemotactic protein 1 (MCP-1), tumor necrosis factor-alpha (TNF-α), interleukin-6(IL-6), nuclear factor kappa-B(NF-κB), NADPH oxidase-2 (NOX2), matrix metalloprotein (MMP-9) and transforming growth factor-beta (TGF-β) levels were assessed to evaluate inflammation, fibrosis and oxidative stress levels in heart after ICH. Macrophages and neutrophils were assessed to evaluate inflammatory cell infiltration in heart after ICH. ICH induced sympathetic excitability, as identified by increased circulating adrenaline, noradrenaline, cortisol levels and β1-AR expression in heart tissue. Metoprolol-treated ICH mice had improved cardiac and neurological function. The suppression of sympathetic overactivity by metoprolol attenuates cardiac inflammation, fibrosis and oxidative stress after ICH. In conclusion, ICH-induced secondary sympathetic overactivity which mediated inflammatory response may play an important role in post-ICH cardiac dysfunction.
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http://dx.doi.org/10.1016/j.autneu.2021.102832DOI Listing
September 2021

Deficiency of Endothelial Nitric Oxide Synthase (eNOS) Exacerbates Brain Damage and Cognitive Deficit in A Mouse Model of Vascular Dementia.

Aging Dis 2021 Jun 1;12(3):732-746. Epub 2021 Jun 1.

1Department of Neurology, Henry Ford Hospital, Detroit, MI-48202, USA.

Vascular Dementia (VaD) accounts for nearly 20% of all cases of dementia. eNOS plays an important role in neurovascular remodeling, anti-inflammation, and cognitive functional recovery after stroke. In this study, we investigated whether eNOS regulates brain damage, cognitive function in mouse model of bilateral common carotid artery stenosis (BCAS) induced VaD. Late-adult (6-8 months) C57BL/6J and eNOS knockout (eNOS-/-) mice were subjected to BCAS (n=12/group) or sham group (n=8/group). BCAS was performed by applying microcoils to both common carotid arteries. Cerebral blood flow (CBF) and blood pressure were measured. A battery of cognitive functional tests was performed, and mice were sacrificed 30 days after BCAS. Compared to corresponding sham mice, BCAS in wild-type (WT) and eNOS-/- mice significantly: 1) induces short term, long term memory loss, spatial learning and memory deficits; 2) decreases CBF, increases ischemic cell damage, including apoptosis, white matter (WM) and axonal damage; 3) increases blood brain barrier (BBB) leakage, decreases aquaporin-4 (AQP4) expression and vessel density; 4) increases microglial, astrocyte activation and oxidative stress in the brain; 5) increases inflammatory factor interleukin-1 receptor-associated kinase-1(IRAK-1) and amyloid beta (Aβ) expression in brain; 6) increases IL-6 and IRAK4 expression in brain. eNOS-/-sham mice exhibit increased blood pressure, decreased iNOS and nNOS in brain compared to WT-sham mice. Compared to WT-BCAS mice, eNOS-/-BCAS mice exhibit worse vascular and WM/axonal damage, increased BBB leakage and inflammatory response, increased cognitive deficit, decreased iNOS, nNOS in brain. eNOS deficit exacerbates BCAS induced brain damage and cognitive deficit.
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http://dx.doi.org/10.14336/AD.2020.0523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139201PMC
June 2021

S-Allylmercapto-N-acetylcysteine ameliorates elastase-induced chronic obstructive pulmonary disease in mice via regulating autophagy.

Biochem Biophys Res Commun 2021 Jul 25;562:83-88. Epub 2021 May 25.

Department of Pharmaceutics, Key Laboratory of Chemical Biology of Ministry of Education, School of Pharmaceutical Sciences, Cheelloo College of Medicine, Shandong University, 44 West Wenhua Road, Jinan, Shandong, 250012, PR China; Key University Laboratory of Pharmaceutics & Drug Delivery Systems of Shandong Province, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, 44 West Wenhua Road, Jinan, Shandong, 250012, PR China; Pediatric Pharmaceutical Engineering Laboratory of Shandong Province, Shandong Dyne Marine Biopharmaceutical Company Limited, Rongcheng, Shandong, 264300, PR China; Chemical Immunopharmaceutical Engineering Laboratory of Shandong Province, Shandong Xili Pharmaceutical Company Limited, Heze, Shandong, 274300, PR China. Electronic address:

Autophagy-impairment is involved in the pathological process of chronic obstructive pulmonary disease (COPD), and relates to inflammation and emphysema in lung injury. This study aimed to elucidate the protective effect of S-Allylmercapto-N-acetylcysteine (ASSNAC) against COPD via regulating the autophagy. Firstly, porcine pancreatic elastase (PPE)-induced COPD model in A549 cells was established, and ASSNAC was verified to alleviate the autophagy-impairment from the results of western blotting analysis of LC3BⅡ/Ⅰ and monodansylcadaverine (MDC) staining of autophagosome. Secondly, Balb/c mice were stimulated by PPE to induce the COPD model in vivo. The histological analysis of lung tissues presented that ASSNAC could alleviate the lung injury induced by PPE. Thirdly, the secretions of NO, TNF-α and IL-1β in serum and BALF were reduced by ASSNAC compared with the PPE group. Finally, the mechanism of therapeutic effects of ASSNAC against COPD through regulating the autophagy-impairment was clarified. That is, ASSNAC inhibits the phosphorylation of PI3K/Akt/mTOR signaling pathways. In a word, this research provides a reference for ASSNAC to be an effective drug for pulmonary diseases.
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http://dx.doi.org/10.1016/j.bbrc.2021.05.047DOI Listing
July 2021

Detection of chlorophyll fluorescence parameters of potato leaves based on continuous wavelet transform and spectral analysis.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Oct 2;259:119768. Epub 2021 Apr 2.

Key Laboratory of Modern Precision Agriculture System Integration Research, Ministry of Education, China Agricultural University, Beijing 100083, China. Electronic address:

The tuber development and nutrient transportation of potato crops are closely related to canopy photosynthesis dynamics. Chlorophyll fluorescence parameters of photosystem II, especially the maximum quantum yield of primary photochemistry (Fv/Fm), are intrinsic indicators for plant photosynthesis. Rapid detection of Fv/Fm of leaves by spectroscopy method instead of time-consuming pulse amplitude modulation technique could help to indicate potato development dynamics and guide field management. Accordingly, this study aims to extract fluorescence signals from hyperspectral reflectance to detect Fv/Fm. Hyperspectral imaging system and closed chlorophyll fluorescence imaging system were applied to collect the spectral data and values of Fv/Fm of 176 samples. The spectral data were decomposed by continuous wavelet transform (CWT) to obtain wavelet coefficients (WFs). Three mother wavelet functions including second derivative of Gaussian (gaus2), biorthogonal 3.3 (bior3.3) and reverse biorthogonal 3.3 (rbio3.3) were compared and the bior3.3 showed the best correlation with Fv/Fm. Two variable selection algorithms were used to select sensitive WFs of Fv/Fm including Monte Carlo uninformative variables elimination (MC-UVE) algorithm and random frog (RF) algorithm. Then the partial least squares (PLS) regression was used to establish detection models, which were labeled as bior3.3-MC-UVE-PLS and bior3.3-RF-PLS, respectively. The determination coefficients of prediction set of bior3.3-MC-UVE-PLS and bior3.3-RF-PLS were 0.8071 and 0.8218, respectively, and the root mean square errors of prediction set were 0.0181 and 0.0174, respectively. The bior3.3-RF-PLS had the best detection performance and the corresponding WFs were mainly distributed in the bands affected by fluorescence emission (650-800 nm), chlorophyll absorption and reflection. Overall, this study demonstrated the potential of CWT in fluorescence signals extraction and can serve as a guide in the quick detection of chlorophyll fluorescence parameters.
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http://dx.doi.org/10.1016/j.saa.2021.119768DOI Listing
October 2021

The CBL-Interacting Protein Kinase NtCIPK23 Positively Regulates Seed Germination and Early Seedling Development in Tobacco ( L.).

Plants (Basel) 2021 Feb 8;10(2). Epub 2021 Feb 8.

Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China.

CBL-interacting protein kinase (CIPK) family is a unique group of serine/threonine protein kinase family identified in plants. Among this family, and its homologs in some plants are taken as a notable group for their importance in ions transport and stress responses. However, there are limited reports on their roles in seedling growth and development, especially in plants. In this study, , a homolog of was cloned from . Expression analysis showed that is mainly expressed in the radicle, hypocotyl, and cotyledons of young tobacco seedlings. The transcriptional level of changes rapidly and spatiotemporally during seed germination and early seedling growth. To study the biological function of at these stages, the overexpressing and CRISPR/Cas9-mediated knock-out () tobacco lines were generated. Phenotype analysis indicated that knock-out of significantly delays seed germination and the appearance of green cotyledon of young tobacco seedling. Overexpression of promotes cotyledon expansion and hypocotyl elongation of young tobacco seedlings. The expression of in hypocotyl is strongly upregulated by darkness and inhibited under light, suggesting that a regulatory mechanism of light might underlie. Consistently, a more obvious difference in hypocotyl length among different tobacco materials was observed in the dark, compared to that under the light, indicating that the upregulation of contributes greatly to the hypocotyl elongation. Taken together, not only enhances tobacco seed germination, but also accelerate early seedling growth by promoting cotyledon greening rate, cotyledon expansion and hypocotyl elongation of young tobacco seedlings.
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http://dx.doi.org/10.3390/plants10020323DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915007PMC
February 2021

S-allylmercaptocysteine inhibits mucin overexpression and inflammation via MAPKs and PI3K-Akt signaling pathways in acute respiratory distress syndrome.

Pharmacol Res 2020 09 20;159:105032. Epub 2020 Jun 20.

School of Pharmaceutical Sciences, Cheelloo College of Medicine, Shandong University, 44 West Wenhua Road, Jinan, Shandong 250012, PR China; Shandong Key University Laboratory of Pharmaceutics & Drug Delivery Systems, 44 West Wenhua Road, Jinan, Shandong 250012, PR China; Shandong Engineering & Technology Research Center for Jujube Food and Drug, 44 West Wenhua Road, Jinan, Shandong 250012, PR China. Electronic address:

Cytokine storm is an important cause of acute respiratory distress syndrome and multiple organ failure. Excessive secretion and accumulation of mucins on the surface of airway cause airway obstruction and exacerbate lung infections. MUC5AC and MUC5B are the main secreted mucins and overexpressed in various inflammatory responses. S-allylmercaptocysteine, a water-soluble organic sulfur compound extracted from garlic, has anti-inflammatory and anti-oxidative effects for various pulmonary diseases. The aim of this work was to investigate the therapeutic effects of SAMC on mucin overproduction and inflammation in 16HBE cells and LPS-induced ARDS mice. Results show that SAMC treatment ameliorated inflammatory cell infiltration and lung histopathological changes in the LPS-induced ARDS mice. SAMC also inhibited the expressions of MUC5AC and MUC5B, decreased the production of pro-inflammatory markers (IL-6, TNF-α, CD86 and IL-12) and increased the production of anti-inflammatory markers (IL-10, CD206 and TGF-β). These results confirm that SAMC had potential beneficial effects on suppressed hyperinflammation and mucin overexpression. Furthermore, SAMC exerted the therapeutic effects through the inhibition of phosphorylation of MAPKs and PI3K-Akt signaling pathways in the 16HBE cells and mice. Overall, our results demonstrate the effects of SAMC on the LPS-induced mucin overproduction and inflammation both in the 16HBE cells and mice.
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http://dx.doi.org/10.1016/j.phrs.2020.105032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305891PMC
September 2020

Nitrate Signaling, Functions, and Regulation of Root System Architecture: Insights from .

Genes (Basel) 2020 06 9;11(6). Epub 2020 Jun 9.

Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.

Root system architecture (RSA) is required for the acquisition of water and mineral nutrients from the soil. One of the essential nutrients, nitrate (NO), is sensed and transported by nitrate transporters and in the plants. Nitrate transporter 1.1 () is a dual-affinity nitrate transporter phosphorylated at the T101 residue by calcineurin B-like interacting protein kinase (CIPKs); it also regulates the expression of other key nitrate assimilatory genes. The differential phosphorylation (phosphorylation and dephosphorylation) strategies and underlying Ca signaling mechanism of stimulate lateral root growth by activating the auxin transport activity and Ca-ANR1 signaling at the plasma membrane and the endosomes, respectively. NO additionally functions as a signal molecule that forms a signaling system, which consists of a vast array of transcription factors that control root system architecture that either stimulate or inhibit lateral and primary root development in response to localized and high nitrate (NO), respectively. This review elucidates the so-far identified nitrate transporters, nitrate sensing, signal transduction, and the key roles of nitrate transporters and its downstream transcriptional regulatory network in the primary and lateral root development in under stress conditions.
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http://dx.doi.org/10.3390/genes11060633DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348705PMC
June 2020

NH Toxicity, Which Is Mainly Determined by the High NH/K Ratio, Is Alleviated by CIPK23 in .

Plants (Basel) 2020 Apr 14;9(4). Epub 2020 Apr 14.

Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China.

Ammonium (NH) toxicity is always accompanied by ion imbalances, and NH and potassium (K) exhibit a competitive correlation in their uptake and transport processes. In , the typical leaf chlorosis phenotype in the knockout mutant of () is high-NH-dependent under low-K condition. However, the correlation of K and NH in the occurrence of leaf chlorosis in the mutant has not been deeply elucidated. Here, a modified hydroponic experimental system with different gradients of NH and K was applied. Comparative treatments showed that NH toxicity, which is triggered mainly by the high ratio of NH to K (NH/K ≥ 10:1 for ) but not by the absolute concentrations of the ions, results in leaf chlorosis. Under high NH/K ratios, is upregulated abundantly in leaves and roots, which efficiently reduces the leaf chlorosis by regulating the contents of NH and K in plant shoots, while promoting the elongation of primary and lateral roots. Physiological data were obtained to further confirm the role in alleviating NH toxicity. Taken all together, might function in different tissues to reduce stress-induced NH toxicity associated with high NH/K ratios by regulating the NH-K balance in .
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http://dx.doi.org/10.3390/plants9040501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238117PMC
April 2020

Facile fabrication of Hildewintera-colademonis-like hexagonal boron nitride/carbon nanotube composite having light weight and enhanced microwave absorption.

J Colloid Interface Sci 2020 Mar 28;564:454-466. Epub 2019 Dec 28.

R&D Center of Lubricating and Protecting Materials, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China. Electronic address:

Hildewintera-colademononis-like hexagonal boron nitride carbon nanotubes ([email protected]) composites can be fabricated via two steps: a composite structure predesign in a solvent and a subsequent thermal treatment process at high temperature. The as-obtained hildewintera-colademononis-like [email protected] composites contain porous h-BN microrods as stems and CNTs as spines. The densities and specific surface area of these [email protected] composites can be tuned by adjusting the relative amounts of CNTs in the composites, which can reach 0.072 ± 0.0046 g/cm and 583.63 m/g, respectively. These [email protected] composites based absorbers show excellent microwave absorption (MA) properties which have effective frequency absorption width (≤-10 dB) from 2.8 to 18 GHz when the absorber thicknesses are in the range of 1.0-6.0 mm, and the minimum RL values can reach up to -48.45 dB for [email protected] based absorber with an absorber thickness only of 1.4 mm. Moreover, the widest absorption bandwidth of 4.24 GHz (12.96-17.20 GHz) can be obtained for [email protected] based absorber when the absorber thickness is 1.6 mm. Therefore, these hildewintera-colademononis-like [email protected] composites are expected to be used as microwave absorption materials as they are lightweight and have broad absorption bands and strong absorption with thin thickness. This facile and controllable fabrication process offers a new strategy for designing and fabricating diverse h-BN/carbon based composites for different applications.
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http://dx.doi.org/10.1016/j.jcis.2019.12.124DOI Listing
March 2020

Oxygen Reduction Activity and Stability of Composite Pd/Co-Nanofilms/C Electrocatalysts in Acid and Alkaline Media.

Front Chem 2018 29;6:596. Epub 2018 Nov 29.

Department of Energy and Chemical Engineering, College of Chemistry and Chemical Engineering, Henan Polytechnic University, Jiaozuo, China.

The morphology tuning of Pd and Pd-M nanoparticles is one of the significant strategies to control the catalytic activity toward oxygen reduction reaction (ORR). In this study, composite Pdx/Co-nanofilms/C electrocatalysts of Pd nanoparticles implanted onto Co nanofilms were synthesized on an immiscible ionic liquid (IL)/water interface for ORR. The Pd nanoparticles implanted onto Co nanofilms show a marked distortion of crystal lattice and surface roughness. These Pdx/Co-nanofilms/C electrocatalysts exhibit enhanced activity for ORR compared with Pd/C and PdxCo/C catalysts in both acid and alkaline solutions, in which the Pd3/Co-nanofilms/C catalyst displays the highest ORR mass activity. The superior ORR mass activities of the fabricated Pdx/Co-nanofilms/C catalysts may be mainly attributed to their larger catalytic areas, which are conferred by the rough surface of Pd nanoparticles with a distorted crystal lattice, and the synergistic effect between the surface Pd atoms and the 2D Co nanofilm substrate. The relationship between ORR mass activity and Pd/Co atom ratio varies in different electrolytes. Furthermore, by using proper heat-treatment methods, the Pdx/Co-nanofilms/C catalysts exhibit improved cycling stability compared with pure Pd/C catalyst after extended potential cycling.
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http://dx.doi.org/10.3389/fchem.2018.00596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6281968PMC
November 2018
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