Publications by authors named "Luis A Rivera-Burgos"

3 Publications

  • Page 1 of 1

Accounting for heading date gene effects allows detection of small-effect QTL associated with resistance to Septoria nodorum blotch in wheat.

PLoS One 2022 19;17(5):e0268546. Epub 2022 May 19.

U.S. Department of Agriculture - Agricultural Research Service, North Carolina State University, Raleigh, North Carolina, United States of America.

In humid and temperate areas, Septoria nodorum blotch (SNB) is a major fungal disease of common wheat (Triticum aestivum L.) in which grain yield is reduced when the pathogen, Parastagonospora nodorum, infects leaves and glumes during grain filling. Foliar SNB susceptibility may be associated with sensitivity to P. nodorum necrotrophic effectors (NEs). Both foliar and glume susceptibility are quantitative, and the underlying genetics are not understood in detail. We genetically mapped resistance quantitative trait loci (QTL) to leaf and glume blotch using a double haploid (DH) population derived from the cross between the moderately susceptible cultivar AGS2033 and the resistant breeding line GA03185-12LE29. The population was evaluated for SNB resistance in the field in four successive years (2018-2021). We identified major heading date (HD) and plant height (PH) variants on chromosomes 2A and 2D, co-located with SNB escape mechanisms. Five QTL with small effects associated with adult plant resistance to SNB leaf and glume blotch were detected on 1A, 1B, and 6B linkage groups. These QTL explained a relatively small proportion of the total phenotypic variation, ranging from 5.6 to 11.8%. The small-effect QTL detected in this study did not overlap with QTL associated with morphological and developmental traits, and thus are sources of resistance to SNB.
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May 2022

Sorghum () Gene Links Lignin Biosynthesis to Folate Metabolism.

Genes (Basel) 2021 04 28;12(5). Epub 2021 Apr 28.

Department of Agronomy, Purdue University, 915 W. State St., West Lafayette, IN 47907-2054, USA.

Genetic analysis of brown midrib sorghum () mutant lines assembled in our program has previously shown that the mutations fall into four allelic groups, , , or . Causal genes for allelic groups , and , have since been identified. In this report, we provide evidence for the nature of the mutation. This was accomplished by introgressing each of the four alleles into nine different genetic backgrounds. Polymorphisms from four resequenced bulks of sorghum introgression lines containing either mutation, relative to those of a resequenced bulk of the nine normal midrib recurrent parent lines, were used to locate their respective causal mutations. The analysis confirmed the previously reported causal mutations for and but failed in the case of -bulk due to a mixture of mutant alleles at the locus among members of that mutant bulk. In the -bulk, a common G → A mutation was found among all members in . This gene encodes a putative folylpolyglutamate synthase with high homology to maize The brown midrib phenotype co-segregated with this point mutation in two separate F populations. Furthermore, an additional variant allele at this locus obtained from a TILLING population also showed a brown midrib phenotype, confirming this locus as .
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April 2021

Biomass and Bioenergy Potential of Brown Midrib Sweet Sorghum Germplasm.

Front Plant Sci 2019 20;10:1142. Epub 2019 Sep 20.

Department of Agronomy, Purdue University, West Lafayette, IN, United States.

Public appetite for fossil fuels continues to drive energy prices and foment the build-up of intractable environmental problems. Ethanol (ETOH) production from lignocellulosic biomass grown in marginal lands offers a sustainable alternative without diverting arable land from food and feed production. The quantity and quality of lignocellulosic biomass can be enhanced by the abundant genetic diversity for biomass production as well as stem sugar and lignin composition in sorghum ( L. Moench). The objective of this study was to assess yield and quality of lignocellulosic biomass enhancement for ethanol production potential in a population of sorghum derived from two cultivars with contrasting biomass yield and compositional traits. We tested 236 recombinant inbred lines (RIL) of sorghum in a randomized complete block design (RCBD) with two replications for lignocellulosic biomass performance and determined hemicellulose, cellulose and lignin concentrations through detergent fiber analysis (DFA). The stover compositional values were used to estimate theoretical ethanol yield (ETOH on a mass basis) and production (ETOH on an area basis). Results showed that RIL carrying the brown midrib mutation had significantly higher theoretical glucose recovery (released glucose from cellulose, > 200 g kg). Those carrying both mutations, had high theoretical ethanol yield (>400 L ton) and high theoretical ethanol production (>14,500 L ha). Lignin concentration was determined as most reliable predictor (R = 0.67) for glucose recovery. Lignin and stem sugar concentrations (R = 0.46 and 0.35, respectively) were good predictors for ethanol yield. Stover yield traits (R = 0.89) were most important determinants for ethanol production. Our findings suggest that careful breeding of sorghum for genetic enhancement of biomass quantity and quality could double lignocellulosic ethanol yields.
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September 2019