Publications by authors named "Lu-Yan Qin"

13 Publications

  • Page 1 of 1

Concentrations and health risks of heavy metals in five major marketed marine bivalves from three coastal cities in Guangxi, China.

Ecotoxicol Environ Saf 2021 Oct 28;223:112562. Epub 2021 Jul 28.

Department of Cell Biology, School of Life Sciences, and School of Medicine, Guangxi University, Nanning 530004, China. Electronic address:

Seafood consumption provides essential elements to humans while also posing risks to human health. A total of 2610 individuals of five edible marine bivalve species (Ruditapes philippinarum, Paphia undulata, Meretrix meretrix, Sinonovacula constricta and Meretrix lyrata) were randomly sampled from six farmer markets in three cities (Beihai, Qinzhou and Fangchenggang) in the southernmost coastal region of China. The concentrations of heavy metals (Cu, Pb, Zn, Cd, Cr, Hg and As) were determined by inductively coupled plasma mass spectrometry (ICP-MS). The estimated daily intake (EDI), target hazard quotient (THQ), total hazard index (HI), and target cancer risk (TR) were calculated to evaluate potential human health risks from bivalve consumption. The mean concentrations of metals in the tissues of bivalves descended in the order Zn > Cu > As > Cd > Cr >Pb > Hg in descending order, and the concentrations varied substantially among the five bivalves. Heavy metal concentrations in edible tissues of most bivalve samples were below the safety limits set by national and international regulations, and there were significant correlations between certain metal concentrations. The EDI values for each metal in each bivalve were significantly lower than the corresponding PTDI (provisional tolerable daily intake) values. Health risk assessment showed that although there is no noncarcinogenic health risk for local residents exposed to individual or combined metals from these bivalves, there is a carcinogenic risk from Cd and Cr exposure. Thus, in the long term, monitoring and controlling bivalve consumption will be important. Although current accumulation levels of bivalves are safe, continued and excessive lifetime consumption over 70 years may pose a target cancer risk.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112562DOI Listing
October 2021

Aging Induced p53/p21 in Genioglossus Muscle Stem Cells and Enhanced Upper Airway Injury.

Stem Cells Int 2020 4;2020:8412598. Epub 2020 Mar 4.

Oral Biomedical and Engineering Laboratory, Shanghai Stomatological Hospital, Fudan University, Shanghai 200001, China.

Aging of population brings related social problems, such as muscle attenuation and regeneration barriers with increased aging. Muscle repair and regeneration depend on muscle stem cells (MuSCs). Obstructive sleep apnea (OSA) rises in the aging population. OSA leads to hypoxia and upper airway muscle injury. However, little is known about the effect of increasing age and hypoxia to the upper airway muscle. The genioglossus (GG) is the major dilator muscle to keep the upper airway open. Here, we reported that muscle fiber and MuSC function declined with aging in GG. Increasing age also decreased the migration and proliferation of GG MuSCs. p53 and p21 were high expressions both in muscle tissue and in GG MuSCs. We further found that hypoxia inhibited GG MuSC proliferation and decreased myogenic differentiation. Then, hypoxia enhanced the inhibition effect of aging to proliferation and differentiation. Finally, we investigated that hypoxia and aging interact to form a vicious circle with upregulation of p53 and p21. This vicious hypoxia plus aging damage accelerated upper airway muscle injury. Aging and hypoxia are the major damage elements in OSA patients, and we propose that the damage mechanism of hypoxia and aging in GG MuSCs will help to improve upper airway muscle regeneration.
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http://dx.doi.org/10.1155/2020/8412598DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073476PMC
March 2020

[Cone-beam CT study of the characteristics of midpalatal suture at different age groups in a southern China population].

Shanghai Kou Qiang Yi Xue 2018 Apr;27(2):190-194

Xiangya Stomatological Hospital, Central South University. Changsha 410008, China.

Purpose: The purpose of this cone-beam CT (CBCT) based study was to investigate the stages of palatal suture at different age groups as well as the bone density of the palatal suture in a south Chinese population.

Methods: The CBCT data of 113 patients with an age range from 4 to 36 years old were selected. All of them were reported to have normal growth. CBCT image data were selected in accordance with the inclusion criteria. By using Angelieri method, the palate was divided into 5 groups, and Chad Evans Larson bone density ratio was calculated for each patient. One-way ANOVA and LSD analysis were used to evaluate the average bone density ratio of the mid-palatal at each age stage as well as the mean value of palatal suture bone density at different stages with SPSS 22.0 software package.

Results: By comparing the density ratio of mid-palatal suture for each age group, no significant difference was noticed for the same stage regardless of the age (stage B, C and D) (P>0.05), significant difference was noticed between C and D(P<0.05),no significant difference was noticed for the suture density between group A and B as well as group C and D (P>0.05), significant difference was found for the suture density between A/C, A/D, A/E, B/C, B/D, B/E, C/D, and C/E groups(P<0.05).

Conclusions: The mid-palatal suture density in children under 15 years may belong to stage A, B or C , which is significantly lower than group D and E; Patients of a palatal stage C stay within an age range from 11 to 18. No inner group difference is noticed inside group C. The average suture density of group C is significantly larger than group A and B, whereas smaller than group D and E. The results showed that CBCT can be a useful tool to guide rapid mid-palatal expansion in clinical practice. Patients who have a lower density mid-palatal suture can be expanded.
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April 2018

Effects of the overexpression of IFITM5 and IFITM5 c.-14C>T mutation on human osteosarcoma cells.

Oncol Lett 2017 Jan 23;13(1):111-118. Epub 2016 Nov 23.

Key Laboratory for Rare Disease Research of Shandong, Shandong Medical Biotechnological Center, Shandong Academy of Medical Sciences, Jinan, Shandong 250062, P.R. China.

The present study aimed to investigate the effects of overexpression of interferon-induced transmembrane protein 5 (IFITM5) and IFITM5 c.-14C>T mutation on osteogenic differentiation, and the proliferation, migration and invasion of SaOS2 cells. SaOS2 cells were transfected with plasmids containing wild type IFITM5 (W) or IFITM5 containing the c.-14C>T mutation (MU). The mRNA and protein expression levels of IFITM5 in SaOS2 cells were respectively detected by reverse transcription quantitative polymerase chain reaction and western blotting. The proliferative, migratory and invasive ability of SaOS2 cells was also examined. In addition, the expression levels of osteogenic differentiation markers alkaline phosphatase (ALP), osteocalcin (OCN) and runt-related transcription factor 2 (Runx2) were detected. Mineralized nodules were detected by Alizarin Red S staining and were quantified by measuring absorbance. The mRNA and protein expression levels of IFITM5 were high in cells transfected with IFITM5 and IFITM5 c.-14C>T mutation, and were higher in cells transfected with IFITM5 c.-14C>T mutation. There was no difference in proliferation between the control group (C) and the W and MU groups. However, overexpression of IFITM5 and IFITM5 c.-14C>T mutation increased apoptotic rate, decreased invasive capacity, increased the expression of ALP, OCN and Runx2, and increased the number of mineralized nodules following osteogenic induction. In addition, compared with C and W groups, cells transfected with IFITM5 c.-14C>T mutation exhibited decreased migratory ability. In conclusion, overexpression of IFITM5 and IFITM5 c.-14C>T mutation promotes tumor cell apoptosis, inhibits tumor invasion and promotes osteogenic differentiation. These findings may provide a theoretical basis for the development of a novel treatment method that targets IFITM5, and provides a platform for the potential treatment of human osteosarcoma.
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http://dx.doi.org/10.3892/ol.2016.5426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5244967PMC
January 2017

[Research progress on pathology of osteogenesis imperfecta].

Zhonghua Bing Li Xue Za Zhi 2013 Nov;42(11):780-3

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November 2013

Management of Crouzon syndrome in an adult patient.

J Clin Orthod 2013 Nov;47(11):673-80

Department of Orthodontics, School of Stomatology, Xiangya Stomatology Hospital, Central South University, Xiangya Road, Changsha, Hunan Province, China. [email protected] 163.com.

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November 2013

MicroRNAs: important mediators of ossification.

Chin Med J (Engl) 2012 Nov;125(22):4111-6

Shandong Academy of Medical Sciences, Jinan, Shandong, China.

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November 2012

[A preliminary study on the functional occlusion characters of the patients who had essential root resorption in anterior teeth].

Shanghai Kou Qiang Yi Xue 2010 Jun;19(3):240-3

Department of Stomatology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China.

Purpose: In order to determine the effect of functional occlusion on the essential root resorption, we compared the characters of functional occlusion between the patients who had essential root resorption in anterior teeth and who didn't have.

Methods: Panoramic radiography, periapical films and clinical data were used to diagnose the essential root resorption before the patients received orthodontic treatment. Fifteen patients who had 2-3 degree root resorption were divided into the experimental group, while 15 patients who didn't have were chosen as control group according to their age and sex. The functional occlusion of the cases in the experimental group and control group was checked on Denar Mark II articulator and via clinical examination, and the indexes of functional occlusion during protrusive and lateral movement in two groups were recorded. SPSS 13.0 software package was used for statistical analysis.

Results: The results showed that the number of anterior teeth occlusal interferences during lateral movement in the experimental group was more than that in control group by clinical (P<0.05) or articulator examination (P<0.05), but the number of posterior teeth occlusal interferences didn't have significant difference between the two groups. There was no significant difference in the retrusive range (RCP-ICP) in vertical, anterior-posterior and lateral direction respectively.

Conclusion: It is suggested that as a kind of long-standing and minor trauma, the anterior teeth occlusal interferences during lateral movement might be a cause of essential root resorption in anterior teeth.
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June 2010

Efficient inhibition of hepatitis B virus replication by hepatitis delta virus ribozymes delivered by targeting retrovirus.

Virol J 2010 Mar 17;7:61. Epub 2010 Mar 17.

Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangdong Province, PR China.

Background: Hepatitis delta virus (HDV) ribozyme is an attractive molecular tool that can specifically recognize and catalyze the self-cleavage of the viral RNA phosphodiester backbone. However, a major obstacle in the medical application of the HDV ribozyme is the lack of specificity in the delivery of the ribozyme to defined target cells.

Results: The objective of this study was to determine whether retroviral vectors can deliver the HDV ribozyme into the target cells and to elucidate whether HDV ribozyme plays a role in hepatitis B virus (HBV) replication. In our study, the transduction of helper-free pseudotyped retrovirus, which showed a broad host range, in human hepatoma cells was performed under 2 conditions, that is, in the presence of polymerized human serum albumin (pHSA) and in the absence of pHSA. The transduction ability in the presence of pHSA was higher than in the absence of pHSA. Moreover, HBsAg and HBeAg levels after transductions with pHSA were significantly lower than those in the absence of pHSA, thus indicating that the recombinant retrovirus had HBV-specific cleavage activity and targeted HepG2215 cells.

Conclusions: These data suggest that this system provides a new approach for targeting hepatocytes and has a great potential in gene therapy for HBV infection.
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http://dx.doi.org/10.1186/1743-422X-7-61DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2850903PMC
March 2010

Virus-encoded microRNAs: future therapeutic targets?

Cell Mol Immunol 2006 Dec;3(6):411-9

Shandong Medicinal Biotechnology Center, Shandong Academy of Medical Science, Key Laboratory of Ministry of Health for Biotech-Drug, Jinan 250062, China.

The discovery of microRNAs (miRNAs) is a remarkable breakthrough in the field of molecular genetics, as miRNAs are key actors which regulate gene expression in diverse cellular processes from unicellular yeast to human. The recent discovery of virus-encoded miRNAs indicates that viruses also use this fundamental mode of gene regulation. Research into viral miRNAs function demonstrates that some miRNAs play an important role in regulating both the viral life cycle and the interaction between viruses and their hosts. The first in vivo "antagomir" study provides an exciting first step towards miRNA therapy, and the potential for ultimately designing molecular medicines based on the modulation of miRNAs seems good.
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December 2006

Rapid quantification of hepatitis B virus DNA by real-time PCR using efficient TaqMan probe and extraction of virus DNA.

World J Gastroenterol 2006 Dec;12(45):7365-70

Shandong Medicinal Biotechnology Center, Shandong Academy of Medical Sciences, Key Laboratory of Ministry of Health for Biotech-Drugs, Jinan 250062, China.

Aim: To rapidly quantify hepatitis B virus (HBV) DNA by real-time PCR using efficient TaqMan probe and extraction methods of virus DNA.

Methods: Three standards were prepared by cloning PCR products which targeted S, C and X region of HBV genome into pGEM-T vector respectively. A pair of primers and matched TaqMan probe were selected by comparing the copy number and the Ct values of HBV serum samples derived from the three different standard curves using certain serum DNA. Then the efficiency of six HBV DNA extraction methods including guanidinium isothiocyanate, proteinase K, NaI, NaOH lysis, alkaline lysis and simple boiling was analyzed in sample A, B and C by real-time PCR. Meanwhile, 8 clinical HBV serum samples were quantified.

Results: The copy number of the same HBV serum sample originated from the standard curve of S, C and X regions was 5.7 multiply 10(4)/mL, 6.3 multiply 10(2)/mL and 1.6 multiply 10(3)/mL respectively. The relative Ct value was 26.6, 31.8 and 29.5 respectively. Therefore, primers and matched probe from S region were chosen for further optimization of six extraction methods. The copy number of HBV serum samples A, B and C was 3.49 multiply 10(9)/mL, 2.08 multiply 10(6)/mL and 4.40 multiply 10(7)/mL respectively, the relative Ct value was 19.9, 30 and 26.2 in the method of NaOH lysis, which was the efficientest among six methods. Simple boiling showed a slightly lower efficiency than NaOH lysis. Guanidinium isothiocyanate, proteinase K and NaI displayed that the copy number of HBV serum sample A, B and C was around 10(5)/mL, meanwhile the Ct value was about 30. Alkaline failed to quantify the copy number of three HBV serum samples. Standard deviation (SD) and coefficient variation (CV) were very low in all 8 clinical HBV serum samples, showing that quantification of HBV DNA in triplicate was reliable and accurate.

Conclusion: Real-time PCR based on optimized primers and TaqMan probe from S region in combination with NaOH lysis is a simple, rapid and accurate method for quantification of HBV serum DNA.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4087500PMC
http://dx.doi.org/10.3748/wjg.v12.i45.7365DOI Listing
December 2006

[MBT straight wire for Class II division I malocclusion cases].

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2006 Jun;31(3):411-3

Department of Stomatology, Xiangya Hospital, Central South University, Changsha, China.

Objective: To evaluate the therapeutic effects of MBT straight wire on extraction case of Class II division I.

Methods: Thirty-six class II division I malocclusion patients were treated with MBT straight wire by extracting 4 bicuspid premolars or 2 maxillary bicuspid premolars. The X-ray cephalograms were analyzed before and after the treatment of MBT straight wire.

Results: After the treatment, the overjet was reduced by 6.04 mm (P < 0.01); U1-NA was reduced by 15.43 degrees (P <0.01); and U1-NA (mm) was reduced by 4.71 mm (P <0.01). ANB was significantly reduced (P < 0.05).

Conclusion: MBT straight wire not only shortens the operation but also shows superior effect for Class II division I malocclusion cases.
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June 2006

[Gene chip analysis of mutation of HBV-DNA open reading frame].

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2004 Dec;18(4):373-6

Jinan Infectious Disease Hospital, Jinan 250021, China.

Objective: To detect the mutation of HBV-DNA open reading frame (ORF), provide evidences for clinical diagnosis and treatment.

Methods: By using gene chip technique, HBV-DNA was amplified by PCR, incorporated with fluorescence, hybridized with oligonucleotide to detect the gene sequence of DNA by computer analysis and observe the natural mutation of HBV-DNA ORF.

Results: The mutations of HBV-DNA ORF existed widespread. The rates of mutation on Pre-C 1896, PreC 1814, BCP 1762, BCP 1764, P 528, P 552MI, P 552MV were 23.5%, 3.9%, 55.9%, 53.9%,39.2%, 38.2%, 10.8%, respectively.

Conclusion: The gene chip technique possesses extremely high sensitivity and reliability,it is one of the effective methods to detect gene mutation. The mutation of HBV-DNA has important influence on the stability and progress of the disease, and on the judgement of prognosis.
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December 2004
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