Publications by authors named "Lu-Lu Zhang"

128 Publications

Recognition of the atrioventricular node anatomical structure: Connection between the retroaortic node and the compact node.

J Cardiovasc Electrophysiol 2021 May 30. Epub 2021 May 30.

Department of Cardiology, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China.

Introduction: The complex electrophysiological phenomena related to the atrioventricular node (AVN) are due to its complex anatomical structures. Aside from the inferior nodal extension (INE), other node-like tissues, such as the retroaortic node (RN), have been described less extensively and may also share the mechanism of normal conduction and abnormal conduction in AVN re-entrant tachycardia.

Methods: High-density sections of the entire AVN were obtained from rats and rabbits. Fibrosis was analyzed by Masson's trichrome staining. Connexin (Cx43, Cx40, and Cx45) and ion channel (Na 1.5, Ca 3.1, and HCN4) proteins were immunohistochemically labeled for the analysis of tissue features. Three-dimensional (3D) reconstruction of the AV junction was performed to clarify the relationships among different structures.

Results: The RN expressed the same connexin isoforms as the compact node (CN) and INE. Na 1.5 labeling was observed at low levels in the CN, RN, and INE, where Ca 3.1 and HCN4 were expressed. The CN connected with the RN in a narrow strip pattern at the start of the CN. The RN presented as a shuttle shape and was the only tissue directly connected with the atrium in the anterior septum.

Conclusion: The RN connects with the AVN anatomically, suggesting that direct electrical conduction occurs between them. The entrance of the atria into the AVN is distal to the RN, which may form the fast AVN pathway.
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http://dx.doi.org/10.1111/jce.15117DOI Listing
May 2021

Clinical efficacy and safety of nazartinib for epidermal growth factor receptor mutated non-small cell lung cancer: Study protocol for a prospective, multicenter, open-label.

Medicine (Baltimore) 2021 May;100(21):e25992

Department of Nephrology, Wuhan Fourth Hospital; Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.

Background: Nazartinib is considered a new, permanent, and mutant-selective epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI). It has a demonstrated efficacy to treat patients experiencing EGFR-mutated non-small cell cancer (NSCLC). The present study aims to explore the clinical efficacy and safety of nazartinib in patients experiencing EGFR-mutated NSCLC.

Materials And Methods: The present study is a prospective, multicentre, open-label experiment seeking to assess the clinical safety as well as efficacy of nazartinib in patients suffering from EGFR-mutated NSCLC. The study will randomly divide 78 patients into experimental and control groups using a ratio of 1:1. Additionally, the study will treat the experimental group with nazartinib, and the control group with other chemotherapeutic agents. Besides, the study will treat both the experimental and control groups with standard treatment for a period of 14 days and will be followed up at least 24 weeks. Overall response rate is the major endpoint. Accordingly, the minor endpoints will include progression-free survival, response time, overall survival, and adverse events. Statistical analysis will be performed by SPSS 25.0 software.

Discussion: The study will investigate the clinical safety and efficacy of nazartinib in patients suffering from EGFR-mutated NSCLC. The anticipated results of the study are expected to provide clinical basis for nazartinib to treat patients suffering from EGFR-mutated NSCLC.
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http://dx.doi.org/10.1097/MD.0000000000025992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154402PMC
May 2021

Efficacy and safety of two different adjuvant chemotherapy regimens in combination with concurrent chemoradiotherapy in treating patients with advanced nasopharyngeal carcinoma: A protocol for randomized controlled trial.

Medicine (Baltimore) 2021 May;100(21):e25980

Department of Pathology, Wuhan Fourth Hospital; Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.

Background: Concurrent chemoradiotherapy is widely utilised as a standardized primary method of treatment for patients with advanced nasopharyngeal carcinoma (NPC). However, the combination of concurrent chemoradiotherapy and adjuvant chemotherapy for treating NPC patients remain unclear. Therefore, this study attempts to elucidate the efficiency and safety of concurrent chemoradiotherapy combined with adjuvant chemotherapy (gemcitabine plus cisplatin versus 5-fluorouracil plus cisplatin) for treating patients with NPC.

Materials And Methods: This study is a randomized, multicentral, open-labelled trial to assess the clinical efficiency and safety of using concurrent chemoradiotherapy combined with adjuvant chemotherapy as a therapeutic measure for advanced NPC patients. A total of 50 patients will be randomly assigned into 2 groups, namely treatment-group-one and treatment-group-two. Eligible patients will be administered with concurrent chemoradiotherapy and subsequentially with adjuvant chemotherapy (gemcitabine plus cisplatin or 5-fluorouracil plus cisplatin). Moreover, the primary endpoint is a comparison of progression-free survival between concurrent chemoradiotherapy and subsequentially adjuvant gemcitabine and cisplatin and chemoradiotherapy, which is proceeded by adjuvant 5-fluorouracil and cisplatin in advanced NPC patients. Overall survival, overall response rate, incidence of acute and late toxicity, and adverse events are the minor endpoints. Statistical analyses will be performed with SPSS 25.0 software.

Discussion: The current research evaluates the clinical efficiency and safety of utilising concurrent chemoradiotherapy combined with adjuvant chemotherapy as a therapeutic strategy to treat advanced NPC patients. The work done in this study will provide a clinical basis for concurrent chemoradiotherapy in combination with adjuvant chemotherapy for treating advanced NPC.

Trial Registration: DOI 10.17605/OSF.IO/5UPVM.
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http://dx.doi.org/10.1097/MD.0000000000025980DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154501PMC
May 2021

Magnolol attenuates inflammatory pain by inhibiting sodium currents in mouse dorsal root ganglion neurons.

Inflammopharmacology 2021 May 22. Epub 2021 May 22.

Key Laboratory of Medical Electrophysiology, Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research of Southwest, Medical University, Luzhou, 646000, China.

Voltage-gated sodium channels are currently recognized as one of the targets of analgesics. Magnolol (Mag), an active component isolated from Magnolia officinalis, has been reported to exhibit analgesic effects. The objective of this study was to investigate whether the analgesic effect of Mag was associated with blocking Na channels. Inflammatory pain was induced by the injection of carrageenan into the hind paw of mice. Mag was administered orally. Mechanical hyperanalgesia was evaluated by using von Frey filaments. Na currents and neuronal excitability in acutely isolated mouse dorsal root ganglion (DRG) neurons were recorded with the whole-cell patch clamp technique. Results showed that Mag (10 ~ 40 mg/kg) dose-dependently inhibited the paw edema and reduced mechanical pain in the inflammatory animal model. Injection of carrageenan significantly increased the amplitudes of TTX-sensitive and TTX-resistant Na currents. Compared with the carrageenan group, Mag inhibited the upregulation of two types of Na currents induced by carrageenan in a dose-dependent manner. Mag 40 mg/kg shifted the inactivation curves of two types of Na currents to hyperpolarization and returned to normal animal level without changing their activation curves. Mag 40 mg/kg significantly reduced the percentage of cells firing multiple spikes and inhibited the neuronal hyperexcitability induced by carrageenan. Our data suggest that the analgesic effect of Mag may be associated with a decreased neuronal excitability by blocking Na current.
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http://dx.doi.org/10.1007/s10787-021-00809-8DOI Listing
May 2021

Effect of short-term intake of four sweeteners on feed intake, solution consumption and neurotransmitters release on mice.

J Food Sci Technol 2021 Jun 19;58(6):2227-2236. Epub 2020 Aug 19.

Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070 China.

This study focused on the effect of short-term intake of sweeteners on feed intake, solution consumption and neurotransmitters release on mice. The results showed that the free drinking of 10 mM sucralose solution, 100 mM maltose solution, 3 mM saccharin solution and 3 g/L stevioside solution for 32 days will not affect the normal development of the body weight and feed intake of the mice. The consumption of maltose solution was significantly higher than that of the other sweeteners. The leptin and insulin levels increased significantly after the short-term intake of these four sweeteners. The dopamine (DA) content in the whole brain of the mice increased significantly only in the maltose group. These results indicate that the short-term intake of the preferred concentrations of maltose, stevioside, sucralose and saccharin will not affect the body weight and feed intake of the mice. Mice prefer maltose solution to other sweeteners solutions. The 100 mM maltose solution and 3 mM saccharin solution could result in the oxidative stress on mice after 32 days' short-term intake. Compared with other sweeteners, only sugars that could be broken down into small molecules of glucose might have a positive effect on dopamine levels.
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http://dx.doi.org/10.1007/s13197-020-04733-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076381PMC
June 2021

Screening a Strain of sp. O852 and the Optimization of Fermentation Conditions for -Dihydrocarvone Production.

Molecules 2021 Apr 22;26(9). Epub 2021 Apr 22.

Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Flavors and fragrances have high commercial value in the food, cosmetic, chemical and pharmaceutical industries. It is interesting to investigate the isolation and characterization of new microorganisms with the ability to produce flavor compounds. In this study, a new strain of sp. O852 (accession number CCTCC M2020509) was isolated from decayed navel orange ( (L.) Osbeck), which was proved to be capable of converting limonene to -dihydrocarvone. Besides, the optimization of various reaction parameters to enhance the -dihydrocarvone production in shake flask was performed for sp. O852. The results showed that the yield of -dihydrocarvone reached up to 1 058 mg/L when sp. O852 was incubated using LB-M medium for 4 h at 36 °C and 150 rpm, and the biotransformation process was monitored for 36 h after adding 1680 mg/L limonene/ethanol (final ethanol concentration of 0.8% ()). The content of -dihydrocarvone increased 16 times after optimization. This study provided a basis and reference for producing -dihydrocarvone by biotransformation.
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http://dx.doi.org/10.3390/molecules26092432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122266PMC
April 2021

Polyphenols from Induce Apoptosis of HepG2 Cells via Inactivation of ERK and AKT Signaling Pathways.

Evid Based Complement Alternat Med 2021 23;2021:8841706. Epub 2021 Mar 23.

Department of Bioengineering, College of Food Science, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China.

The extract of has been proved to have antitumor activity. However, the underlying mechanism remains unclear. This study aimed to elucidate the mechanism of apoptosis of HepG2 cells induced by polyphenols from (PBPs). The results revealed that PBPs inhibited the proliferation of HepG2 cells in a dose-dependent and time-dependent manner. Flow cytometry analysis showed that PBPs increased the apoptosis ratio of HepG2 cells significantly. PBPs increased intracellular reactive oxygen species (ROS) production and decreased intracellular superoxide dismutase (SOD) level of HepG2 cells. PBPs induced cell cycle arrest at G1 phase. Western blotting showed that PBPs upregulated the ratio of /-2 and the expression level of -3, and activated 53 in HepG2 cells. The inhibition of proliferative relative signals (protein kinase B, PKB/AKT) and survival relative signals (extracellular signal-regulated kinase, ERK) were also observed in PBP-treated HepG2 cells. Our findings suggest that apoptosis of HepG2 cells induced by PBPs is mitochondria-mediated via inactivation of ERK and AKT signaling pathways.
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http://dx.doi.org/10.1155/2021/8841706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009708PMC
March 2021

Development and validation of a prognostic nomogram for the pre-treatment prediction of early metachronous metastasis in endemic nasopharyngeal carcinoma: a big-data intelligence platform-based analysis.

Ther Adv Med Oncol 2020 21;12:1758835920978132. Epub 2020 Dec 21.

Department of Molecular Diagnostics, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng East Road, Guangzhou 510060, People's Republic of China.

Background: Early failure of cancer treatment generally indicates a poor prognosis. Here, we aim to develop and validate a pre-treatment nomogram to predict early metachronous metastasis (EMM) in nasopharyngeal carcinoma (NPC).

Methods: From 2009 to 2015, a total of 9461 patients with NPC (training cohort:  = 7096; validation cohort:  = 2365) were identified from an institutional big-data research platform. EMM was defined as time to metastasis within 2 years after treatment. Early metachronous distant metastasis-free survival (EM-DMFS) was the primary endpoint. A nomogram was established with the significant prognostic factors for EM-DMFS determined by multivariate Cox regression analyses in the training cohort. The Harrell Concordance Index (C-index), area under the receiver operator characteristic curve (AUC), and calibration curves were applied to evaluate this model.

Results: EMM account for 73.5% of the total metachronous metastasis rate and is associated with poor long-term survival in NPC. The final nomogram, which included six clinical variables, achieved satisfactory discriminative performance and significantly outperformed the traditional tumor-node-metastasis (TNM) classification for predicting EM-DMFS: C-index: 0.721 0.638,  < 0.001; AUC: 0.730 0.644,  < 0.001. The calibration curves showed excellent agreement between the predicted and actual EM-DMFS. The nomogram can stratify patients into three risk groups with distinct EM-DMFS (2-year DMFS: 96.8% 90.1% 80.3%,  < 0.001). A validation cohort supported the results. The three identified risk groups are correlated with the efficacy of different treatment regimens.

Conclusion: Our established nomogram can reliably predict EMM in patients with NPC and might aid in formulating risk-adapted treatment decisions and personalized patient follow-up strategies.
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http://dx.doi.org/10.1177/1758835920978132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758560PMC
December 2020

[Transfer Factor and Health Risk Assessment of Heavy Metals in a Soil-Crop System in a High Incidence Area of Nasopharyngeal Carcinoma, Guangdong].

Huan Jing Ke Xue 2020 Dec;41(12):5579-5588

Guangdong Provincial Key Laboratory of Geological Process and Mineral Resources Exploration, Sun Yat-sen University, Guangzhou 510275, China.

In order to reveal the transfer factor and perform health risk assessments of heavy metals in soil-crop systems in the high incidence area of nasopharyngeal carcinoma (NPC) in Guangdong province of China, the farmland system of Sihui City in the high incidence area of NPC was selected as the research object, and rice, lettuce, and corresponding soil samples were collected. As, Cu, Hg, Mn, Ni, Pb, and Cd in the soil and crop samples were analyzed. Based on the contents and chemical forms of seven heavy metals, the environmental pollution, bioavailability, and transfer factors of heavy metals in the soil-crop system were assessed using statistical analyses, pollution index evaluations, and transfer factor methods, and the health risks of adults and children in the study area were assessed using the health risk assessment model recommended by the U.S. Environmental Protection Agency. The results showed that the farmland soil in the study area was basically clean (=0.43); Cd and Mn mainly existed in a bioavailable state, Hg mainly existed in a potentially available state, and As Cu, Ni, and Pb mainly existed in a residual state. The lettuce was safe (=0.48), while the pollution index of rice (=7.66) was higher than that of lettuce, and the main polluting element was Pb (PI=10.25). The results of soil pollution assessments are not completely consistent with those of crop pollution assessments, so they should be combined with the bioavailability of heavy metals and crop effects for correlation analyses. Cd and Cu are more easily absorbed by lettuce, while Cd, Cu, and As are more easily enriched by rice. Special attention should be paid to Cd and Cu pollution in farmland soils, and As pollution should be of focus in paddy fields. In the study area, the non-carcinogenic risk index (HI) value of edible lettuce for adults and children was less than 1 and the average value of the total carcinogenic risk index (Risk) of edible lettuce was less than 1×10. Therefore, the health risk of edible local lettuce was within the acceptable range. The average HI index of rice for adults and children was more than 1 and the main non-carcinogenic factor was Pb; the risk index of rice was more than 1×10, and the main carcinogenic factor was As. Rice consumption in the study area will cause certain health risks, and the threat to adults is greater than that to children. Therefore, As in rice may be related to the high incidence of NPC in Sihui City. It is suggested that the remediation of heavy metals in farmland soils be strengthened or that residents be forbidden to plant or eat local rice and other crops with greater health risks.
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http://dx.doi.org/10.13227/j.hjkx.202005053DOI Listing
December 2020

[Bioaccumulation Characteristics of Quinolones (QNs) in Dominant Fish Species and Their Correlation with Environmental Factors in Baiyangdian Lake].

Huan Jing Ke Xue 2020 Dec;41(12):5470-5479

Pollution Prevention Biotechnology Laboratory of Hebei Province, School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050000, China.

In recent years, quinolone antibiotics (QNs), which easily bioaccumulate in aquatic organisms, have been widely detected in lake ecosystems, and the bioaccumulation and trophic transfer behavior are obviously spatiotemporally different. In this study, the bioaccumulation and trophic transfer behavior of fourteen QNs in nine dominant fish species were studied, the correlation with environmental factors was analyzed, and the health risk of QNs was evaluated in Baiyangdian Lake. The results showed that the mass concentrations of ∑QNs in water varied from 0.7400 to 1590 ng·L. Furthermore, the detected frequencies of flumequine (FLU), oxolinic acid (OXO), and ofloxacin (OFL) were higher, and the average mass concentration of FLU was the highest. The content of ∑QNs in fish ranged from 17.1 to 146 ng·g, and the average contents of ciprofloxacin (CIP) and OFL were higher. The bioaccumulation factors (BAF) were in the range of 96.2 (BAF)-489 (BAF) L·kg, indicating the bioaccumulation of QNs was low in dominant fish species. The trophic magnification factors (TMF) of five QNs (enrofloxacin (ENR), FLU, marbofloxacin (MAR), norfloxacin (NOR), and OFL) varied from 0.714 (TMF) to 1.33 (TMF), indicating ENR exhibited trophic magnification, while FLU, MAR, and ∑QNs exhibited trophic dilution. The results of correlation analysis between environmental parameters and BAF/TMF showed that pH, , SD, DO, COD, TP, TN, NH-N, NO-N, and PO-P were significantly related to the bioaccumulation of QNs in fish. The results of human health risk showed that the hazard quotient (HQ) of CIP (0.0040-0.026) was significantly higher than that of other QNs (≤ 0.0050), and the hazard indices (HI) ranged from 0.0010 to 0.035, indicating a high level of health risk. Therefore, to reduce the health risk, the standard and residue limits of QNs should be set in Baiyangdian Lake.
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http://dx.doi.org/10.13227/j.hjkx.202005129DOI Listing
December 2020

Development of a Nomogram Model for Treatment of Nonmetastatic Nasopharyngeal Carcinoma.

JAMA Netw Open 2020 12 1;3(12):e2029882. Epub 2020 Dec 1.

Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Department of Molecular Diagnostics, Collaborative Innovation Center for Cancer Medicine, Guangzhou, People's Republic of China.

Importance: Because of tumor heterogeneity, overall survival (OS) differs significantly among individuals with nasopharyngeal carcinoma (NPC), even among those with the same clinical stage. Relying solely on TNM staging to guide treatment remains imperfect.

Objectives: To establish a comprehensive nomogram to estimate individualized OS and to explore stratified treatment regimens for risk subgroups in nonmetastatic NPC.

Design, Setting, And Participants: This cohort study included 8093 patients diagnosed with NPC at a single center in China from April 2009 to December 2015. The sample was split into a training cohort (5398 participants [66.7%]) and validation cohort (2695 [33.3%]). Data were analyzed in May 2020.

Exposures: Age, T stage, N stage, Epstein-Barr virus (EBV) DNA level, serum lactate dehydrogenase (LDH) levels, and albumin (ALB) levels.

Main Outcomes And Measures: The primary end point was OS. The nomogram for estimating OS was generated based on multivariate Cox proportional hazards regression. The performance of the nomogram was quantified using Harrell concordance index (C index), the area under the curve (AUC) of the receiver operating characteristic curve, and a calibration curve. OS rates were established using the Kaplan-Meier method, and intersubgroup differences were examined by the log-rank test.

Results: Among the 8093 participants, 5688 (70.3%) were men, and the median age at diagnosis was 45 years (range, 7-85 years). Six variables (age, T stage, N stage, EBV DNA levels, LDH levels, and ALB levels) were identified through multivariate Cox regression and incorporated into a nomogram to estimate OS. The resulting nomogram showed excellent discriminative ability and significantly outperformed the eighth edition of the American Joint Committee on Cancer/Union for International Cancer Control TNM staging system for estimating OS (C index, 0.716 [95% CI, 0.698-0.734] vs 0.643 [95% CI, 0.624-0.661]; P < .001; AUC, 0.717 [95% CI, 0.698-0.737] vs 0.643 [95% CI, 0.623-0.662]; P < .001), and the calibration curves showed satisfactory agreement between the actual and nomogram-estimated OS rates. The validation cohort confirmed the results. Patients were stratified into 4 risk groups based on the 25th, 50th, and 75th percentile score values estimated from the nomogram. The 4 nomogram-defined risk groups demonstrated significantly different intergroup OS (3-year OS rates: risk group 1, 1328 of 1345 [98.7%]; risk group 2, 1289 of 1341 [96.1%]; risk group 3, 1222 of 1321 [92.5%]; risk group 4, 1173 of 1391 [84.3%]; P < .001). These risk groups were associated with the efficacy of different treatment regimens. For example, for risk group 4, induction chemotherapy with concurrent chemoradiotherapy was associated with a significantly better OS than concurrent chemoradiotherapy (log-rank P = .008) and intensity-modulated radiotherapy alone (log-rank P < .001).

Conclusions And Relevance: In this study, the proposed nomogram model enabled individualized prognostication of OS and could help to guide risk-adapted treatment for patients with nonmetastatic NPC.
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http://dx.doi.org/10.1001/jamanetworkopen.2020.29882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733160PMC
December 2020

Prognostic value of radiation interruption in different periods for nasopharyngeal carcinoma patients in the intensity-modulated radiation therapy era.

Cancer Med 2021 01 27;10(1):143-155. Epub 2020 Oct 27.

Department of Radiation Oncology, State Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat-Sen University Cancer Center, Collaborative Innovation Center for Cancer Medicine, Guangzhou, People's Republic of China.

We aimed to investigate the prognostic value of radiation interruptions at different times on the overall survival (OS) and disease-free survival (DFS) of patients with nasopharyngeal carcinoma receiving intensity-modulated radiation therapy. Totally, 4510 patients were identified from a well-established big-data intelligence platform. Optimal interruption thresholds were identified using Recursive partitioning analyses. Actuarial rates were plotted using the Kaplan-Meier method and were compared using the log-rank test. Patients with preceding interruptions ≥1 d (5-year OS, 89.6% vs. 85.7%, p < 0.001; 5-year DFS, 81.4% vs. 76.4%, p < 0.001), or latter interruptions ≥4 d (88.4% vs. 82.3%, p < 0.001; 79.2% vs. 75.1%, p = 0.006) showed significant detrimental effects on OS and DFS than patients without those interruptions. However, no significant lower survival was identified in latter interruptions ≥1 d (5-year OS: 89.0% vs. 86.7%, p = 0.053; 5-year DFS, 80.2% vs. 77.8%, p = 0.080). Latter interruptions ≥4 d was an independent unfavorable prognostic factor for OS (HR, 1.404; 95% CI, 1.143-1.723, p = 0.001) and DFS (HR, 1.351; 95% CI, 1.105-1.652, p = 0.003) in multivariate analysis. Radiation interruptions longer than 3 days that occurred in the latter period of treatment with IMRT were independent factors in poorer survival. Efforts are needed to minimize radiation interruptions and improve the timely provision of treatment.
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http://dx.doi.org/10.1002/cam4.3580DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826475PMC
January 2021

Long Noncoding RNA TINCR-Mediated Regulation of Acetyl-CoA Metabolism Promotes Nasopharyngeal Carcinoma Progression and Chemoresistance.

Cancer Res 2020 12 16;80(23):5174-5188. Epub 2020 Oct 16.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.

Frontier evidence suggests that dysregulation of long noncoding RNAs (lncRNA) is ubiquitous in all human tumors, indicating that lncRNAs might have essential roles in tumorigenesis. Therefore, an in-depth study of the roles of lncRNA in nasopharyngeal carcinoma (NPC) carcinogenesis might be helpful to provide novel therapeutic targets. Here we report that lncRNA TINCR was significantly upregulated in NPC and was associated positively with poor survival. Silencing TINCR inhibited NPC progression and cisplatin resistance. Mechanistically, TINCR bound ACLY and protected it from ubiquitin degradation to maintain total cellular acetyl-CoA levels. Accumulation of cellular acetyl-CoA promoted lipid biosynthesis and histone H3K27 acetylation, which ultimately regulated the peptidyl arginine deiminase 1 (PADI1)-MAPK-MMP2/9 pathway. In addition, insulin-like growth factor 2 mRNA-binding protein 3 interacted with TINCR and slowed its decay, which partially accounted for TINCR upregulation in NPC. These findings demonstrate that TINCR acts as a crucial driver of NPC progression and chemoresistance and highlights the newly identified TINCR-ACLY-PADI1-MAPK-MMP2/9 axis as a potential therapeutic target in NPC. SIGNIFICANCE: TINCR-mediated regulation of a PADI1-MAPK-MMP2/9 signaling pathway plays a critical role in NPC progression and chemoresistance, marking TINCR as a viable therapeutic target in this disease.
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http://dx.doi.org/10.1158/0008-5472.CAN-19-3626DOI Listing
December 2020

A novel Rap-Phr system in Bacillus velezensis NAU-B3 regulates surfactin production and sporulation via interaction with ComA.

Appl Microbiol Biotechnol 2020 Dec 12;104(23):10059-10074. Epub 2020 Oct 12.

Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.

Several quorum sensing systems occurring in Bacillus subtilis, e.g. Rap-Phr systems, were reported to interact with major regulatory proteins, such as ComA, DegU, and Spo0A, in order to regulate competence, sporulation, and synthesis of secondary metabolites. In this study, we characterized a novel Rap-Phr system, RapA4-PhrA4, in Bacillus velezensis NAU-B3. We found that the rapA4 and phrA4 genes were co-transcribed in NAU-B3. When rapA4 was expressed in the heterologous host Bacillus subtilis OKB105, surfactin production and sporulation were severely inhibited. However, when the phrA4 was co-expressed, the RapA4 activity was inhibited. The transcription of the surfactin synthetase srfA gene and sporulation-related genes were also regulated by the RapA4-PhrA4 system. In vitro results obtained from electrophoretic mobility shift assay (EMSA) proved that RapA4 inhibits ComA binding to the promoter of the srfA operon, and the PhrA4 pentapeptide acts as anti-activator of RapA4. We also found that the F24 residue plays a key role in RapA4 function. This study indicated that the novel RapA4-PhrA4 system regulates the surfactin synthesis and sporulation via interaction with ComA, thereby supporting the bacterium to compete and to survive in a hostile environment. KEY POINTS: •Bacillus velezensis NAU-B3 has a novel Rap-Phr quorum sensing system, which does not occur in model strains Bacillus subtilis 168 and B. velezensis FZB42. •RapA4-PhrA4 regulates surfactin production and sporulation. •RapA4-PhrA4 interacts with the ComA protein from ComP/ComA two-component system.
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http://dx.doi.org/10.1007/s00253-020-10942-zDOI Listing
December 2020

Lycorine inhibits tumor growth of human osteosarcoma cells by blocking Wnt/β-catenin, ERK1/2/MAPK and PI3K/AKT signaling pathway.

Am J Transl Res 2020 15;12(9):5381-5398. Epub 2020 Sep 15.

Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education, Chongqing Medical University Chongqing 400016, People's Republic of China.

Osteosarcoma (OS) is the most common type of primary bone cancer. Even with advances in early diagnosis and aggressive treatment, the overall prognosis for OS remains to be further elevated. Lycorine was an isoquinoline alkaloid mainly existed in the bulb of lyco salvia miltiorrhiza and was shown to inhibit several types of cancer. In the present study, we investigated the anti-OS activity of lycorine and the possible underlying mechanism. We found that lycorine inhibited cell proliferation of human OS cells while had lower cytotoxcity against normal cells, and triggered cell cycle arrest at the G1/S transition. Moreover, we validated that lycorine promoted apoptosis via death receptor pathway and mitochondrial pathway, suppressed migration and invasion by reversing epithelial mesenchymal transition (EMT) and suppressing the degradation of extracellular matrix (ECM) . In addition, orthotopic implantation model of 143B OS cells further confirmed that lycorine suppressed OS growth and lung metastasis . Mechanically, lycorine reduced the protein level of β-catenin and its' downstream molecule c-Myc. Furthermore, lycorine also decreased the phosphorylation of ERK1/2 and AKT. Together, our results reveal that lycorine may inhibit tumor growth of OS cells possibly through suppressing Wnt/β-catenin, ERK1/2 and PI3K/AKT signaling pathway.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7540099PMC
September 2020

Effect of short-term intake of high- and low-concentrations of sucrose solution on the neurochemistry of male and female mice.

Food Funct 2020 Oct;11(10):9103-9113

College of Food Science and Technology, Huazhong Agricultural University, Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan 430070, China.

The effect of short-term intake of high- and low-concentrations of sucrose solution on the neurochemistry of male and female mice was studied. The body weight, feed intake, sucrose solution consumption and brain monoamine neurotransmitters were determined after 34 days' intake of 1% and 8% sucrose solutions. The gene expression and protein levels related to dopamine and opioids were also determined. The results showed that the intake of 1% and 8% sucrose solution for 34 days did not cause significant changes in the weight development of both male and female mice. The preference for sucrose varies with sex. Both males and females had greater preference for the high concentration sucrose solution than the low concentration sucrose solution. The continuous intake of sucrose stimulated the release of monoamine neurotransmitters (DA, 5-HT, NE) in the brains of mice, and the reward effect of 8% sucrose solution is significantly higher than that of 1% sucrose solution. The sex of mice did not affect the release of neurotransmitters. The gene expressions of D1 and D2 were up-regulated in the 1% sucrose group of male mice, while the OPRM1 gene expression was down-regulated. The expression of these three genes in the 8% sucrose group of male mice was all down-regulated, while the gene expressions of D1 and D2 in the 1% and 8% sucrose group (p < 0.05) of female mice were both up-regulated.
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http://dx.doi.org/10.1039/d0fo02214dDOI Listing
October 2020

Convalescent Plasma Rescued a Severe COVID-19 Patient with Chronic Myeloid Leukemia Blast Crisis and Myelofibrosis

Turk J Haematol 2021 02 29;18(1):74-76. Epub 2020 Sep 29.

Taihe Hospital Affiliated to Xi’an Jiaotong University Health Science Center, Department of Hematology, Shiyan, China

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http://dx.doi.org/10.4274/tjh.galenos.2020.2020.0400DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927443PMC
February 2021

Targeted inhibition of calpain in mitochondria alleviates oxidative stress-induced myocardial injury.

Acta Pharmacol Sin 2021 Jun 23;42(6):909-920. Epub 2020 Sep 23.

Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China.

The protein levels and activities of calpain-1 and calpain-2 are increased in cardiac mitochondria under pathological conditions including ischemia, diabetes, and sepsis, and transgenic overexpression of mitochondrial-targeted calpain-1 induces dilated heart failure, which underscores an important role of increased calpain in mitochondria in mediating myocardial injury. However, it remains to be determined whether selective inhibition of calpain in mitochondria protects the heart under pathological conditions. In this study, we generated transgenic mice overexpressing mitochondrial-targeted calpastatin in cardiomyocytes. Their hearts were isolated and subjected to global ischemia/reperfusion. Hyperglycemia was induced in the transgenic mice by injections of STZ. We showed that transgenic calpastatin was expressed exclusively in mitochondria isolated from their hearts but not from other organs including skeletal muscle and lung tissues. Transgenic overexpression of mitochondrial-targeted calpastatin significantly attenuated mitochondrial oxidative stress and cell death induced by global ischemia/reperfusion in isolated hearts, and ameliorated mitochondrial oxidative stress, cell death, myocardial remodeling and dysfunction in STZ-treated transgenic mice. The protective effects of mitochondrial-targeted calpastatin were correlated with increased ATP5A1 protein expression and ATP synthase activity in isolated hearts subjected to global ischemia/reperfusion and hearts of STZ-treated transgenic mice. In cultured rat myoblast H9c2 cells, overexpression of mitochondrial-targeted calpastatin maintained the protein levels of ATP5A1 and ATP synthase activity, prevented mitochondrial ROS production and decreased cell death following hypoxia/reoxygenation, whereas upregulation of ATP5A1 or scavenging of mitochondrial ROS by mito-TEMPO abrogated mitochondrial ROS production and decreased cell death. These results confirm the role of calpain in myocardial injury, suggesting that selective inhibition of calpain in myocardial mitochondria by mitochondrial-targeted calpastatin is an effective strategy for alleviating myocardial injury and dysfunction in cardiac pathologies.
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http://dx.doi.org/10.1038/s41401-020-00526-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149722PMC
June 2021

Effect and mechanism of high-fat diet on the preference for sweeteners on mice.

J Sci Food Agric 2021 Mar 29;101(5):1844-1853. Epub 2020 Sep 29.

College of Food Science and Technology, Huazhong Agricultural University, Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan, China.

Background: Male Kunming mice were divided into a normal diet group (control group) and a high-fat diet group (HF group) (185 g·kg protein, 600 g·kg fat and 205 g·kg carbohydrate). After 8 weeks' feeding, behavioral indicators and biochemical indicators in serum were determined. The double-bottle preference experiment was used to study the preferences of mice for five sweeteners. The monoamine neurotransmitter content, gene expression related to dopamine (DA), and opioid receptors were also determined.

Results: The body weight of the mice in the HF group increased significantly (P < 0.05) after 36 days compared with the control group. The feed intake of the HF group increased sharply in the first 12 days, and then it became basically unchanged. The preference of the HF group for all of the five sweeteners was highly significantly lower (P < 0.01) than that of the control group. Depression-related behavior was observed in the HF group mice. The triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDLC) content in the HF group were very much higher (P < 0.01) than those of the control group. The gene expression related to DA and opioid receptor in the HF group was significantly lower than that of the control group, except for preproenkephalin (PENK).

Conclusions: In summary, this study suggested that a long-term high-fat diet could result in a decrease in the preference for sweeteners and could result in a state of reward hypofunction in mice. © 2020 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.10798DOI Listing
March 2021

The enhancement of the perception of saltiness by Sichuan pepper oleoresin in a NaCl model solution.

Food Res Int 2020 10 24;136:109581. Epub 2020 Jul 24.

Food and Agriculture Standardization Institute, China National Institute of Standardization, Beijing 102200, China.

Health concerns related to the intake of salt have necessitated the investigation into NaCl reduction by examining the cross-modal interaction between the perception of saltiness and pungency elicited by Sichuan pepper oleoresin (Spo). The category scale and the generalized Labeled Magnitude Scale (gLMS) were used to determine the degree to which Spo enhanced the perception of saltiness in the NaCl model solution. Sixty-eight participants were divided into the hyposensitive, semi-sensitive, and hypersensitive groups according to individual exponent. The power functions of saltiness under different pungency carriers were obtained. The level of enhancement varied between the different sensitivity groups and pungency carriers. In the hypersensitive group, the low and strong pungency carriers effectively enhanced the perception of saltiness at low to moderate, and moderate to strong NaCl solutions, respectively. In the semi-sensitive group, low and moderate pungency carriers induced additive effect in the perception of saltiness at full and moderately strong NaCl solutions, respectively. However, the additive effect was inadequate in the hypo-sensitive group. Therefore, the low pungency solution was more feasible for enhancing in the perception of saltiness, while the maximum NaCl reduction percentages corresponded to the hypersensitive and semi-sensitive groups at 38.61% and 39.06%, respectively. This research not only provided insight into the effect of pungency on the perception of saltiness as it related to individual sensitivity, but also presented valuable information regarding flavor when developing food with reduced salt content.
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http://dx.doi.org/10.1016/j.foodres.2020.109581DOI Listing
October 2020

[Extraction technology, composition analysis and antioxidant and antimicrobial activities of volatile oil from fenugreek leaves].

Zhongguo Zhong Yao Za Zhi 2020 Jul;45(13):3161-3168

College of Pharmacy, Qinghai Nationalities University Xining 810007, China Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province Xining 810007, China.

To define the extraction process, main components and antioxidative and antimicrobial activities of volatile oil from fenugreek(Trigonella foenum-graecum) leaves and its active substance basis. Response surface methodology was used for optimum supercritical CO_2 extraction conditions of essential oil from fenugreek leaves. The main components of volatile oil were analyzed by GC-MS, its antioxidant activity was evaluated by measuring the scavenging ability of 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, ABTS) free radical, and the antimicrobial effect of volatile oil was evaluated by K-B paper AGAR diffusion method. The results showed that the optimal extraction temperature was 50 ℃, the extraction time was 89 min, and the extraction pressure was 35 MPa. Under the conditions, the optimum extracting yield of volatile oil was 1.72%,which was about 1.5 times higher than that of the conventional steam distillation. A total of 52 compounds were found based on reference substance retention time and GC-MS fragmentation information or the existing literatures, and the major compounds were oleic acid(9.65%), carveol(9.41%), n-hexadecanoic acid(9.1%), linoleic acid(6.95%), methyl linolenate(5.4%), petroselinic acid(5.3%), testosterone(3.4%), sotolon(1.75%). The volatile oil of fenugreek showed moderate antioxidant activities in DPPH assay(IC_(50) value of 0.473 mg·mL~(-1)) and ABTS test(IC_(50) value of 0.107 mg·mL~(-1)). The oil had a stronger antimicrobial activity in vitro. MIC of the volatile oil ranged from 0.375 to 1.5 mg·mL~(-1). The results showed that the optimized volatile oil extraction process was stable, and the extraction yield was high. Fenugreek leaves contained a variety of volatile components, with obvious antioxidant and antibacterial activities. This study provides a certain theoretical basis for the comprehensive development and utilization of fenugreek.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200523.301DOI Listing
July 2020

Risk stratification for nasopharyngeal carcinoma: a real-world study based on locoregional extension patterns and Epstein-Barr virus DNA load.

Ther Adv Med Oncol 2020 12;12:1758835920932052. Epub 2020 Jun 12.

Department of Molecular Diagnostics, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng East Road, Guangzhou, 510060, People's Republic of China.

Aim: The present study aimed to evaluate the combined value of locoregional extension patterns (LEPs) and circulating cell-free Epstein-Barr virus (cf EBV) DNA for risk stratification of locoregionally advanced nasopharyngeal carcinoma (LA-NPC) to better guide therapeutic strategies.

Methods: A total of 7227 cases of LA-NPC were reviewed retrospectively and classified into six groups according to their LEP (ascending, descending, or mixed type) and pre-treatment cf EBV-DNA load (⩾ <4000 copy/ml). Using a supervised statistical clustering approach, patients in the six groups were clustered into low, intermediate, and high-risk clusters. Progression-free survival (PFS), overall survival (OS), distant metastasis-free survival (DMFS), and locoregional relapse-free survival (LRRFS) were calculated using the Kaplan-Meier method and differences were compared using the log-rank test.

Results: Survival curves for the low, intermediate, and high-risk clusters were significantly different for all endpoints. The 5-year survival rate for the low, intermediate, and high-risk clusters, respectively, were: PFS (83.5%, 73.2%, 62.6%,  < 0.001), OS (91.0%, 82.7%, 73.2%,  < 0.001), DMFS (92.3%, 83.0%, 73.4%,  < 0.001), and LRRFS (91.0%, 88.0%, 83.3%,  < 0.001). The risk clusters acted as independent prognostic factors for all endpoints. Among the patients in the high-risk cluster, neoadjuvant chemotherapy combined with concurrent chemoradiotherapy (CCRT) significantly improved the patients 5-year PFS (66.4% 57.9%,  = 0.014), OS (77.6% 68.6%;  < 0.002), and DMFS (76.6% 70.6%;  = 0.028) compared with those treated with CCRT.

Conclusion: Our results could facilitate the development of risk-stratification and risk-adapted therapeutic strategies for patients with LA-NPC.
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http://dx.doi.org/10.1177/1758835920932052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7294474PMC
June 2020

miR-448-3p alleviates diabetic vascular dysfunction by inhibiting endothelial-mesenchymal transition through DPP-4 dysregulation.

J Cell Physiol 2020 12 15;235(12):10024-10036. Epub 2020 Jun 15.

Department of Geriatrics, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

Diabetes mellitus (DM) often causes vascular endothelial damage and alters vascular microRNA (miR) expression. miR-448-3p has been reported to be involved in the development of DM, but whether miR-448-3p regulates diabetic vascular endothelial dysfunction remains unclear. To investigate the molecular mechanism of diabetic vascular endothelial dysfunction and the role of miR-448-3p therein, Sprague-Dawley rats were injected with streptozotocin (STZ) to establish diabetic animal model and the rat aortic endothelial cells were treated with high glucose to establish diabetic cell model. For the treatment group, after the induction of diabetes, the miR-448-3p levels in vivo and in vitro were upregulated by adeno-associated virus serotype 2 (AAV2)-miR-448-3p injection and miR-448-3p mimic transfection, respectively. Our results showed that AAV2-miR-448-3p injection alleviated the body weight loss and blood glucose level elevation induced by STZ injection. The miR-448-3p level was significantly decreased and the dipeptidyl peptidase-4 (DPP-4) messenger RNA level was increased in diabetic animal and cell models, which was reversed by miR-448-3p treatment. Moreover, the diabetic rats exhibited endothelial damage and endothelial-mesenchymal transition (EndMT), while AAV2-miR-448-3p injection relieved those situations. In vitro experiments demonstrated that miR-448-3p overexpression in endothelial cells alleviated endothelial damage by inhibiting EndMT through blocking the transforming growth factor-β/Smad pathway. We further proved that miR-448-3p negatively regulated DPP-4 by binding to its 3'-untranslated region, and DPP-4 overexpression reversed the effect of miR-448-3p overexpression on EndMT. Overall, we conclude that miR-448-3p overexpression inhibits EndMT via targeting DPP-4 and further ameliorates diabetic vascular endothelial dysfunction, indicating that miR-448-3p may serve as a promising therapeutic target for diabetic endothelial dysfunction.
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http://dx.doi.org/10.1002/jcp.29817DOI Listing
December 2020

Effects of Mindfulness-based Interventions on Dementia Patients: A Meta-analysis.

West J Nurs Res 2020 12 14;42(12):1163-1173. Epub 2020 May 14.

Xiangya School of Nursing, Central South University, Changsha, China.

This study aims to systematically evaluate the efficacy of mindfulness-based intervention (MBI) in improving mental health and quality of life for people with dementia. Comprehensive literature search was performed using the PubMed, EMBASE, Web of Science, Cochrane Library, and CINAHL databases from their inception till June 26, 2019. In total, nine articles met the eligibility criteria and were included. The results of the meta-analysis showed a statistically significant decrease in depressive symptoms (SMD = -0.39, 95% CI: - 0.62 to - 0.15), in people with dementia who were treated with MBI. However, there were no significant improvements in anxiety, stress, or quality of life. These findings suggest that MBI is a promising alternative to conventional interventions in the treatment of depression among dementia patients and warrant further study.
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http://dx.doi.org/10.1177/0193945920916750DOI Listing
December 2020

Genomic and Transcriptomic Study for Screening Genes Involved in the Limonene Biotransformation of DSM 62840.

Front Microbiol 2020 22;11:744. Epub 2020 Apr 22.

Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China.

α-Terpineol has been widely used in daily chemical, pharmaceutical, food, and flavor industries due to its pleasant odor with high economic value and pharmacological action. Our previous study showed that DSM 62840 was an efficient biocatalyst for the transformation of limonene to α-terpineol. Thus, it was meaningful to explore the genome features and the gene expression differences of strain DSM 62840 during limonene biotransformation, and the detailed bioconversion pathways. In this study, the functional genes related to limonene bioconversion were investigated using genome and transcriptome sequences analysis. The results showed that the DSM 62840 genome was estimated to be 29.09 Mb and it encoded 9,086 protein-encoding genes. The most annotated genes were associated to some protein metabolism and energy metabolism functions. When the threshold for differentially expressed genes (DEGs) was set at twofold ratio, a total of 4,128, and 4,148 DEGs were identified in P_L_12h (limonene-treated condition) compared with P_0h (blank) and P_12h (limonene-untreated blank), respectively. Among them, the expression levels of genes involved in the biosynthesis of secondary metabolites, energy metabolism and ATP-binding cassette (ABC) transporters were significantly altered during the biotransformation. And the reliability of these results was further confirmed by quantitative real-time polymerase chain reaction (RT-qPCR). Moreover, we found that the enzyme participated in limonene biotransformation was inducible. This enzyme was located in the microsome, and it was inhibited by cytochrome P450 inhibitors. This indicated that the cytochrome P450 may be responsible for the limonene bioconversion. Several differentially expressed cytochrome P450 genes were further identified, such as PDIDSM_85260 and PDIDSM_67430, which were significantly up-regulated with limonene treatment. These genes may be responsible for converting limonene to α-terpineol. Totally, the genomic and transcriptomic data could provide valuable information in the discovery of related-genes which was involved in limonene biotransformation, pathogenicity of fungi, and investigation of metabolites and biological pathways of strain DSM 62840.
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http://dx.doi.org/10.3389/fmicb.2020.00744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188761PMC
April 2020

Value of thrombus imaging in predicting the outcomes of patients with large-vessel occlusive strokes after endovascular therapy.

Neurol Sci 2020 Jun 21;41(6):1451-1458. Epub 2020 Feb 21.

Department of neurology, The First Affiliated Hospital of Soochow University, No.899, Pinghai Road, Suzhou, 215000, Jiangsu, China.

Background: Acute ischemic stroke leads to serious long-term disability and high mortality, especially in patients with large-vessel occlusive strokes. Nowadays, endovascular therapy is considered as an alternative treatment for these patients. Several studies have used thrombus characteristics based on non-contrast computed tomography (NCCT) and computed tomography angiography (CTA) to predict prognosis in ischemic stroke. We conducted a systematic review to identify potential imaging predictive factors for successful recanalization and improved clinical outcome after endovascular therapy in patients with large-vessel occlusion (LVO) in anterior arterial circulation.

Methods: The PubMed databases were searched for related studies reported between September 18, 2009, and September 18, 2019.

Results: We selected 11 studies on revascularization and 12 studies on clinical outcome. Patients with thrombus of higher Hounsfield unit (HU), shorter length, higher clot burden score, and increased thrombus permeability may achieve higher recanalization and improved clinical outcome, but the matter is still under debate.

Conclusion: Imaging of thrombus can be used as an aseessment tool to predict the outcomes and it needs further studies in the future.
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http://dx.doi.org/10.1007/s10072-020-04296-7DOI Listing
June 2020

Effects of different sweeteners on behavior and neurotransmitters release in mice.

J Food Sci Technol 2020 Jan 21;57(1):113-121. Epub 2019 Aug 21.

1College of Food Science and Technology, Huazhong Agricultural University, Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan, 430070 China.

Four natural sweeteners (sucrose, stevioside, maltose and xylitol) and six artificial sweeteners (acesulfame, sucralose, aspartame, cyclamate, saccharin and neotame) were used to study the effects of different sweeteners on the behavior and neurotransmitter release of mice with two-bottle preference experiments. The results showed that very significant preference behavior for 8% sucrose solution, 0.3% stevioside solution, 10 mM acesulfame, 10 mM sucralose and 10 mM aspartame solutions ( < 0.01) was observed on mice. Long-term exposure of sucrose solution and acesulfame solution can affect the behavioral indicators such as solution consumption, feed intake, body weight and the release of neurotransmitters in mice. The solution consumption and the release of neurotransmitters were significantly greater ( < 0.05) than that of the control group (water group), but there was no significant difference in feed intake. The acesulfame-A and acesulfame-B groups had no significant difference on the consumption of solution and feed intake, but there was significant difference in the release of neurotransmitters. The result also showed that different sweetener solutions with similar sweetness had the same effect on the neurotransmitters release, and it can be inferred that mice have an addictive behavioral characteristic to sucrose.
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http://dx.doi.org/10.1007/s13197-019-04036-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952499PMC
January 2020

MicroRNAs and their targeted genes associated with phase changes of stem explants during tissue culture of tea plant.

Sci Rep 2019 12 27;9(1):20239. Epub 2019 Dec 27.

Zhejiang University Tea Research Institute, Hangzhou, 310058, P.R. China.

Elucidation of the molecular mechanism related to the dedifferentiation and redifferentiation during tissue culture will be useful for optimizing regeneration system of tea plant. In this study, an integrated sRNAome and transcriptome analyses were carried out during phase changes of the stem explant culture. Among 198 miRNAs and 8001 predicted target genes, 178 differentially expressed miRNAs and 4264 potential targets were screened out from explants, primary calli, as well as regenerated roots and shoots. According to KEGG analysis of the potential targets, pathway of "aminoacyl-tRNA biosynthesis", "proteasome" and "glutathione metabolism" was of great significance during the dedifferentiation, and pathway of "porphyrin and chlorophyll metabolism", "mRNA surveillance pathway", "nucleotide excision repair" was indispensable for redifferentiation of the calli. Expression pattern of 12 miRNAs, including csn-micR390e, csn-miR156b-5p, csn-miR157d-5p, csn-miR156, csn-miR166a-3p, csn-miR166e, csn-miR167d, csn-miR393c-3p, csn-miR394, csn-miR396a-3p, csn-miR396 and csn-miR396e-3p, was validated by qRT-PCR among 57 differentially expressed phase-specific miRNAs. Validation also confirmed that regulatory module of csn-miR167d/ERF3, csn-miR156/SPB1, csn-miR166a-3p/ATHB15, csn-miR396/AIP15A, csn-miR157d-5p/GST and csn-miR393c-3p/ATG18b might play important roles in regulating the phase changes during tissue culture of stem explants.
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http://dx.doi.org/10.1038/s41598-019-56686-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934718PMC
December 2019

N/P-Dual-Doped Carbon-Coated NaV(PO)OF Microspheres as a High-Performance Cathode Material for Sodium-Ion Batteries.

ACS Appl Mater Interfaces 2020 Jan 8;12(3):3670-3680. Epub 2020 Jan 8.

School of Materials Science and Engineering, State Key Laboratory of Material Processing and Die & Mould Technology , Huazhong University of Science and Technology , 1037 Luoyu Road , Wuhan , Hubei 430074 , China.

NaV(PO)OF (NVPOF) is attracting great interest due to its large capacity and high working voltage. However, poor electronic conductivity limits the electrochemical performance of NVPOF. Herein, we fabricate N/P-dual-doped carbon-coated NVPOF microspheres (labeled as [email protected]/N/C) via a hydrothermal process followed by heat treatment. This microsphere-structured [email protected]/N/C composite has a relatively high tap density of 1.22 g/cm. TEM and XPS results reveal that the dual-doped carbon layer is tightly coated on the NVPOF surface due to the bridging effect of P and has a good protective effect on NVPOF. Density functional theory (DFT) calculations confirm that a N/P-dual-doped carbon layer is advantageous to achieve higher electronic conductivity and lower migration activation energy than those of the undoped and single N- or P-doped carbon layer. As a cathode material for a sodium-ion battery (SIB), [email protected]/N/C exhibits high capacity (128 mAh/g at 0.5 C and 122 mAh/g at 2 C) and ultralong cycle performance (only 0.037% capacity fading rate per cycle in 500 cycles at 2 C). We believe that the [email protected]/N/C composite is appealing for high-performance SIBs with large energy density.
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http://dx.doi.org/10.1021/acsami.9b20490DOI Listing
January 2020