Publications by authors named "Lu Zhou"

586 Publications

Potential lncRNA Biomarkers for HBV-Related Hepatocellular Carcinoma Diagnosis Revealed by Analysis on Coexpression Network.

Biomed Res Int 2021 15;2021:9972011. Epub 2021 Oct 15.

Center for Systemic Inflammation Research (CSIR), School of Preclinical Medicine, Youjiang Medical University for Nationalities, Baise City, Guangxi Province, China.

Background: Increasing evidence demonstrated that long noncoding RNA (lncRNA) could affect inflammatory tumor immune microenvironment by modulating gene expression and could be used as a biomarker for HBC-related hepatocellular carcinoma (HCC) but still needs further research. The aim of the present study was to determine an lncRNA signature for the diagnosis of HBV-related HCC.

Methods: HBV-related HCC expression profiles (GSE55092, GSE19665, and GSE84402) were abstracted from the GEO (Gene Expression Omnibus) data resource, and R package limma and RobustRankAggreg were employed to identify common differentially expressed genes (DEGs). Using machine learning, optimal diagnostic lncRNA molecular markers for HBV-related HCC were identified. The expression of candidate lncRNAs was cross-validated in GSE121248, and an ROC (receiver operating characteristic) curve of lncRNA biomarkers was carried out. Additionally, a coexpression network and functional annotation was built, after which a PPI (protein-protein interaction) network along with module analysis were conducted with the Cytoscape open source software.

Result: A total of 38 DElncRNAs and 543 DEmRNAs were identified with a fold change larger than 2.0 and a value < 0.05. By machine learning, AL356056.2, AL445524.1, TRIM52-AS1, AC093642.1, EHMT2-AS1, AC003991.1, AC008040.1, LINC00844, and LINC01018 were screened out as optional diagnostic lncRNA biosignatures for HBV-related HCC. The AUC (areas under the curve) of the SVM (support vector machine) model and random forest model were 0.957 and 0.904, respectively, and the specificity and sensitivity were 95.7 and 100% and 94.3 and 86.5%, respectively. The results of functional enrichment analysis showed that the integrated coexpressed DEmRNAs shared common cascades in the p53 signaling pathway, retinol metabolism, PI3K-Akt signaling cascade, and chemical carcinogenesis. The integrated DEmRNA PPI network complex was found to be comprised of 87 nodes, and two vital modules with a high degree were selected with the MCODE app.

Conclusion: The present study identified nine potential diagnostic biomarkers for HBV-related HCC, all of which could potentially modulated gene expression related to inflammatory conditions in the tumor immune microenvironment. The functional annotation of the target DEmRNAs yielded novel evidence in evaluating the precise functions of lncRNA in HBV-related HCC.
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http://dx.doi.org/10.1155/2021/9972011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536424PMC
October 2021

The Microbiome in Autoimmune Liver Diseases: Metagenomic and Metabolomic Changes.

Front Physiol 2021 8;12:715852. Epub 2021 Oct 8.

Department of Gastroenterology and Hepatology, Tianjin Medical University General Hospital, Tianjin, China.

Recent studies have identified the critical role of microbiota in the pathophysiology of autoimmune liver diseases (AILDs), including autoimmune hepatitis (AIH), primary biliary cholangitis (PBC), and primary sclerosing cholangitis (PSC). Metagenomic studies reveal significant decrease of gut bacterial diversity in AILDs. Although profiles of metagenomic vary widely, is commonly enriched in AIH, PBC, and PSC. Apart from gut microbiome, the oral and bile microbiome seem to be associated with these diseases as well. The functional analysis of metagenomics suggests that metabolic pathways changed in the gut microbiome of the patients. Microbial metabolites, including short-chain fatty acids (SCFAs) and microbial bile acid metabolites, have been shown to modulate innate immunity, adaptive immunity, and inflammation. Taken together, the evidence of host-microbiome interactions and in-depth mechanistic studies needs further accumulation, which will offer more possibilities to clarify the mechanisms of AILDs and provide potential molecular targets for the prevention and treatment in the future.
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http://dx.doi.org/10.3389/fphys.2021.715852DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531204PMC
October 2021

Natural Language Processing Algorithms for Normalizing Expressions of Synonymous Symptoms in Traditional Chinese Medicine.

Evid Based Complement Alternat Med 2021 11;2021:6676607. Epub 2021 Oct 11.

Beijing University of Chinese Medicine, School of Traditional Chinese Medicine, Beijing 100029, China.

Background: The modernization of traditional Chinese medicine (TCM) demands systematic data mining using medical records. However, this process is hindered by the fact that many TCM symptoms have the same meaning but different literal expressions (i.e., TCM synonymous symptoms). This problem can be solved by using natural language processing algorithms to construct a high-quality TCM symptom normalization model for normalizing TCM synonymous symptoms to unified literal expressions.

Methods: Four types of TCM symptom normalization models, based on natural language processing, were constructed to find a high-quality one: (1) a text sequence generation model based on a bidirectional long short-term memory (Bi-LSTM) neural network with an encoder-decoder structure; (2) a text classification model based on a Bi-LSTM neural network and sigmoid function; (3) a text sequence generation model based on bidirectional encoder representation from transformers (BERT) with sequence-to-sequence training method of unified language model (BERT-UniLM); (4) a text classification model based on BERT and sigmoid function (BERT-Classification). The performance of the models was compared using four metrics: accuracy, recall, precision, and F1-score.

Results: The BERT-Classification model outperformed the models based on Bi-LSTM and BERT-UniLM with respect to the four metrics.

Conclusions: The BERT-Classification model has superior performance in normalizing expressions of TCM synonymous symptoms.
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http://dx.doi.org/10.1155/2021/6676607DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8523248PMC
October 2021

LIPUS inhibits inflammation and catabolism through the NF-κB pathway in human degenerative nucleus pulposus cells.

J Orthop Surg Res 2021 Oct 18;16(1):619. Epub 2021 Oct 18.

Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, No. 1 Shiyou Street, Yuzhong District, Chongqing, 400010, China.

Background: Low-intensity pulsed ultrasound (LIPUS) is a safe and noninvasive rehabilitative physical therapy with anti-inflammatory effects. The current study investigated the effect of LIPUS on the inflammation of nucleus pulposus (NP) cells and its underlying mechanism.

Methods: Human NP cells were acquired from lumbar disc herniation tissue samples and cultured for experiments. Human NP cells were treated with LPS and then exposed to LIPUS (15 mW/cm, 30 mW/cm and 60 mW/cm) for 20 min daily for 3 days to determine the appropriate intensity to inhibit the expression of the inflammatory factors TNF-α and IL-1β. The gene and protein expression of aggrecan, collagen II, MMP-3 and MMP-9 was measured by real-time PCR and western blotting, respectively. The activity of the nuclear factor-kappa B (NF-κB) pathway was examined by western blotting and immunofluorescence. After pretreatment with the NF-κB inhibitor PDTC, the expression of TNF-α, IL-1β, MMP-3 and MMP-9 was measured by real-time PCR.

Results: LIPUS at intensities of 15 mW/cm, 30 mW/cm and 60 mW/cm inhibited LPS-induced NP cell expression of the inflammatory factors TNF-α and IL-1β, especially at 30 mW/cm. LIPUS significantly upregulated the gene and protein expression of aggrecan and collagen II and downregulated the gene and protein expression of MMP-3 and MMP-9 in LPS-induced NP cells. The NF-κB signaling pathway was inhibited by LIPUS through inhibiting the protein expression of p-P65 and the translocation of P65 into the nucleus in LPS-induced NP cells. In addition, LIPUS had similar effects as the NF-κB inhibitor PDTC by inhibiting the NF-κB signaling pathway, inflammation and catabolism in LPS-induced human degenerative nucleus pulposus cells.

Conclusion: LIPUS inhibited inflammation and catabolism through the NF-κB pathway in human degenerative nucleus pulposus cells.
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http://dx.doi.org/10.1186/s13018-021-02739-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8522043PMC
October 2021

An Overview of miRNAs Involved in PASMC Phenotypic Switching in Pulmonary Hypertension.

Biomed Res Int 2021 7;2021:5765029. Epub 2021 Oct 7.

Department of Pharmacy, The Second Affiliated Hospital of Nanchang University, 330006 Nanchang, Jiangxi, China.

Pulmonary hypertension (PH) is occult, with no distinctive clinical manifestations and a poor prognosis. Pulmonary vascular remodelling is an important pathological feature in which pulmonary artery smooth muscle cells (PASMCs) phenotypic switching plays a crucial role. MicroRNAs (miRNAs) are a class of evolutionarily highly conserved single-stranded small noncoding RNAs. An increasing number of studies have shown that miRNAs play an important role in the occurrence and development of PH by regulating PASMCs phenotypic switching, which is expected to be a potential target for the prevention and treatment of PH. miRNAs such as miR-221, miR-15b, miR-96, miR-24, miR-23a, miR-9, miR-214, and miR-20a can promote PASMCs phenotypic switching, while such as miR-21, miR-132, miR-449, miR-206, miR-124, miR-30c, miR-140, and the miR-17~92 cluster can inhibit it. The article reviews the research progress on growth factor-related miRNAs and hypoxia-related miRNAs that mediate PASMCs phenotypic switching in PH.
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http://dx.doi.org/10.1155/2021/5765029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516547PMC
October 2021

The Significant Effects of the COVID-19 on Leisure and Hospitality Sectors: Evidence From the Small Businesses in the United States.

Front Public Health 2021 27;9:753508. Epub 2021 Sep 27.

School of Economics, Tianjin Univesity of Commerce, Tianjin, China.

This paper uses the daily seasonally-adjusted data for net revenues and openings of small businesses in the accommodation, food services, leisure, and hospitality sectors in the United States from January 10, 2020, to June 24, 2021. The results from the Dorta-Sanchez bootstrap unit-root test for a random walk with drift show that the COVID-19 crisis has significantly affected revenues and openings of small leisure and hospitality firms. Moreover, the results remain valid when the data for the national level and 51 states are considered.
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http://dx.doi.org/10.3389/fpubh.2021.753508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506250PMC
October 2021

Regulated Polyelectrolyte Nanogels for Enzyme Encapsulation and Activation.

Biomacromolecules 2021 Oct 9. Epub 2021 Oct 9.

Shanghai Key Laboratory of Multiphase Materials Chemical Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China.

Polyelectrolyte (PE) nanogels consisting of cross-linked PE networks integrate the advanced features of both nanogels and PEs. The soft environment and abundant intrinsic charges are of special interest for enzyme immobilization. However, the crucial factors that regulate enzyme encapsulation and activation remain obscure to date. Herein, we synthesized cationic poly (dimethyl aminoethyl methacrylate), PDMAEMA, nanogels with well-defined size and cross-link degrees and fully investigated the effects of different control factors on lipase immobilization. We demonstrate that the cationic PDMAEMA nanogels indeed enable efficient and safe loading of anionic lipase without disturbing their structures. Strong charge interaction achieved by tuning pH and larger particle size are favorable for lipase loading, while the enhanced enzymatic activity demands nanogels with smaller size and a moderate cross-link degree. As such, PDMAEMA nanogels with a hydrodynamic radius of 35 nm and 30% cross-linker fraction display the optimal catalytic efficiency, which is fourfold of that of free lipase. Moreover, the immobilization endows enhanced enzymatic activity in a broad scope of pH, ionic strength, and temperature, demonstrating effective protection and activation of lipase by the designed nanogels. Our study validates the crucial controls of the size and structure of PE nanogels on enzyme encapsulation and activation, and the revealed findings shall be helpful for designing functional PE nanogels and boosting their applications for enzyme immobilization.
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http://dx.doi.org/10.1021/acs.biomac.1c01030DOI Listing
October 2021

Riverside underwater noise pollution threaten porpoises and fish along the middle and lower reaches of the Yangtze River, China.

Ecotoxicol Environ Saf 2021 Dec 6;226:112860. Epub 2021 Oct 6.

The Key Laboratory of Aquatic Biodiversity and Conservation of the Chinese Academy of Sciences, Institute of Hydrobiology of the Chinese Academy of Sciences, Wuhan 430072, PR China. Electronic address:

The Yangtze River exhibits a high biodiversity and plays an important role in global biodiversity conservation. As the world's busiest inland river in regard to shipping, little attention has been paid to underwater noise pollution. In 2017, the underwater noise level in 25 riverside locations along the middle and lower reaches of the Yangtze River mainly at night time were investigated by using passive acoustic monitoring method. Approximately 88% and 40% of the sampled sites exhibit noise levels exceeding the underwater acoustic thresholds of causing responsiveness and temporary threshold shift, respectively, in cetacean. Noise pollution may impose a high impact on fish with physostomous swim bladders and Weberian ossicles, such as silver carp, bighead carp, goldfish and common carp, whereas it may affect fish with physoclistous swim bladders and without Weberian ossicles, such as lake sturgeon and paddlefish, to a lesser extent. Noise levels reductions of approximately 10 and 20 dB were observed in the middle and lower reaches, respectively, of the Yangtze River over the 2012 level. The green development mode of the ongoing construction of green shipping in the Yangtze River Economic Belt, including the development of green shipping lanes, ports, ships and transportation organizations, may account for the alleviated underwater noise pollution. Follow-up noise mitigation endeavors, such as the extension of ship speed restrictions and the study and implementation of the optimal navigation speed in ecologically important areas, are required to further reduce the noise level in the Yangtze River to protect local porpoises and fish.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112860DOI Listing
December 2021

Luminous efficacy enhancement for LED lamps using highly reflective quantum dot-based photoluminescent films.

Opt Express 2021 Aug;29(18):29007-29020

In this study, a strongly reflective and photoluminescent (PL) poly(lactic-co-glycolic acid) quantum dot (QD) hybrid nanofiber (PQHN) structure is introduced to enhance the luminous efficacy of QD-phosphor hybrid white light-emitting diodes (QD-WLEDs). As the thickness of PQHN film increases, the PL is found to continuously increase, exhibiting a maximum peak intensity at 120 μm, which is 1.92 times that at 12 μm, and showing the highest diffuse reflectance of 94.4% at 640 nm. Consequently, while using the QD-WLEDs, the PQHN structure achieves a 53.8% improvement in luminous flux compared with the traditional structure under a similar correlated color temperature (CCT) of 3,540 K, achieving a high luminous efficacy of 202.11 lm W for QD-WLEDs. In addition, the maximum deviation of the CCT is only 11 K when the current is changed from 50 to 950 mA, demonstrating good stability. Therefore, the PQHN films have great potential in lighting systems as a hybrid functional film including light conversion and reflectance.
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http://dx.doi.org/10.1364/OE.431345DOI Listing
August 2021

Amplified Peroxidase-like Activity of Co Using 8-Hydroxyquinoline and Its Application for Ultrasensitive Colorimetric Detection of Clioquinol.

Chem Asian J 2021 Oct 3. Epub 2021 Oct 3.

Institute for Chemical Biology & Biosensing, College of Life Sciences, Qingdao University, 308 Ningxia Road, Qingdao, 266071, P. R. China.

8-Hydroxyquinoline (8HQ) and its derivatives display diverse bioactivities and therapeutic potentials. In this study, we unveiled that 8HQ can boost the peroxidase-like activity of Co in the presence of bicarbonate (HCO ) in neutral pH at room temperature. With 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS) as the substrate, the formed Co /8HQ/HCO complex shows robust catalytic activity with the turnover number (k ) tens to hundreds of times higher than that of Co O and other Co complexes in terms of per cobalt ion. This system was used to design colorimetric sensors for ultrasensitive detection of 8HQ-based drugs by activating the activity of Co . Take detecting clioquinol as an example, a detection limit of 2.4 nM clioquinol with a linear range from 0.01 to 0.2 μM was obtained. This work not only revealed a new kind of ligand that activated the activity of Co , but also provided a facile, low-cost, ultrasensitive, easy-to-use, and universal strategy for sensing various 8HQ-based drugs. Further development of this catalytic system might be beneficial to overcome drug resistance by combined medication.
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http://dx.doi.org/10.1002/asia.202100997DOI Listing
October 2021

Identification, Isolation, and Characterization of Medipeptins, Antimicrobial Peptides From EDOX.

Front Microbiol 2021 14;12:732771. Epub 2021 Sep 14.

Department of Molecular Genetics, University of Groningen, Groningen, Netherlands.

The plant microbiome is a vastly underutilized resource for identifying new genes and bioactive compounds. Here, we used sp. EDOX, isolated from the leaf endosphere of a tomato plant grown on a small farm in the Netherlands. To get more insight into its biosynthetic potential, the genome of sp. EDOX was sequenced and subjected to bioinformatic analyses. The genome sequencing analysis identified strain EDOX as a member of the . analysis for secondary metabolites identified a total of five non-ribosomally synthesized peptides synthetase (NRPS) gene clusters, related to the biosynthesis of syringomycin, syringopeptin, anikasin, crochelin A, and fragin. Subsequently, we purified and characterized several cyclic lipopeptides (CLPs) produced by NRPS, including some of the already known ones, which have biological activity against several plant and human pathogens. Most notably, mass spectrometric analysis led to the discovery of two yet unknown CLPs, designated medipeptins, consisting of a 22 amino acid peptide moiety with varying degrees of activity against Gram-positive and Gram-negative pathogens. Furthermore, we investigated the mode of action of medipeptin A. The results show that medipeptin A acts as a bactericidal antibiotic against Gram-positive pathogens, but as a bacteriostatic antibiotic against Gram-negative pathogens. Medipeptin A exerts its potent antimicrobial activity against Gram-positive bacteria binding to both lipoteichoic acid (LTA) and lipid II as well as by forming pores in membranes. Collectively, our study provides important insights into the biosynthesis and mode of action of these novel medipeptins from EDOX.
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http://dx.doi.org/10.3389/fmicb.2021.732771DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477016PMC
September 2021

The Impact of the Coronavirus Disease 2019 Pandemic on Investor Sentiment-Evidence From A-Share Listed Companies in China.

Front Psychol 2021 13;12:743306. Epub 2021 Sep 13.

School of Economics, Tianjin University of Commerce, Tianjin, China.

In a DID model, this study examines the impact of the coronavirus disease 2019 (COVID-19) pandemic on the investor sentiment in the financial market of China using monthly panel data on newly listed Chinese companies between October 2019 and June 2020. The outbreak of the pandemic is shown to exert a significant negative impact on investor sentiment. A future industry heterogeneity analysis shows that the pandemic has driven up investor sentiment in the pharmaceutical sector while having a significantly negative impact on non-pharmaceutical sectors. The pandemic is shown to have a negative impact on the private sector and foreign-invested sector in China while a significantly positive impact on the state-owned sector. This study contributes to the existing literature on the investigation of how significant the impact of public health emergencies on investor sentiment is.
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http://dx.doi.org/10.3389/fpsyg.2021.743306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473727PMC
September 2021

Identification of Key Genes and Immune Infiltrate in Nonalcoholic Steatohepatitis: A Bioinformatic Analysis.

Biomed Res Int 2021 11;2021:7561645. Epub 2021 Sep 11.

Department of Gastroenterology, General Hospital of Tianjin Medical University, Tianjin, China.

Background: Nonalcoholic steatohepatitis (NASH) can progress to cirrhosis and hepatic carcinoma and is closely associated with changes in the neurological environment. The discovery of new biomarkers would aid in the treatment of NASH.

Methods: Data GSE89632 were downloaded from the Gene Expression Omnibus (GEO) database, and package "limma" was used to identify differentially expressed genes (DEGs) for NASH vs. normal tissues. The STRING database was used to construct a protein-protein interaction (PPI) network, and the Cytoscape software program (Version 3.80) was used to visualize the PPI network and identify key genes. The immune infiltration of NASH was determined using the package "CIBERSORT".

Results: We screened 41 DEGs. GO and KEGG enrichment analyses of the DEGs revealed the enrichment of pathways related to NAFLD steatosis and inflammation. A PPI network analysis was also performed on the DEGs, and seven genes (MYC, CXCL8, FOS, SOCS1, SOCS3, IL6, and PTGS2) were identified as hub genes. An immune infiltration assessment revealed that macrophages M2, memory resting CD4+ T cells, and T cells play important roles in the immune microenvironment of NASH, which may be mediated by the seven identified hub genes.
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http://dx.doi.org/10.1155/2021/7561645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452393PMC
September 2021

The role of vascular dementia associated genes in patients with Alzheimer's disease: A large case-control study in the Chinese population.

CNS Neurosci Ther 2021 Sep 22. Epub 2021 Sep 22.

Department of Neurology, Xiangya Hospital, Central South University, Changsha, China.

Aim: The role of vascular dementia (VaD)-associated genes in Alzheimer's disease (AD) remains elusive despite similar clinical and pathological features. We aimed to explore the relationship between these genes and AD in the Chinese population.

Methods: Eight VaD-associated genes were screened by a targeted sequencing panel in a sample of 3604 individuals comprising 1192 AD patients and 2412 cognitively normal controls. Variants were categorized into common variants and rare variants according to minor allele frequency (MAF). Common variant (MAF ≥ 0.01)-based association analysis was conducted by PLINK 1.9. Rare variant (MAF < 0.01) association study and gene-based aggregation testing of rare variants were performed by PLINK 1.9 and Sequence Kernel Association Test-Optimal (SKAT-O test), respectively. Age at onset (AAO) and Mini-Mental State Examination (MMSE) association studies were performed with PLINK 1.9. Analyses were adjusted for age, gender, and APOE ε4 status.

Results: Four common COL4A1 variants, including rs874203, rs874204, rs16975492, and rs1373744, exhibited suggestive associations with AD. Five rare variants, NOTCH3 rs201436750, COL4A1 rs747972545, COL4A1 rs201481886, CST3 rs765692764, and CST3 rs140837441, showed nominal association with AD risk. Gene-based aggregation testing revealed that HTRA1 was nominally associated with AD. In the AAO and MMSE association studies, variants in GSN, ITM2B, and COL4A1 reached suggestive significance.

Conclusion: Common variants in COL4A1 and rare variants in HTRA1, NOTCH3, COL4A1, and CST3 may be implicated in AD pathogenesis. Besides, GSN, ITM2B, and COL4A1 are probably involved in the development of AD endophenotypes.
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http://dx.doi.org/10.1111/cns.13730DOI Listing
September 2021

BH5 Protects Tomato Plants Against by Production of Specific Antifungal Compounds.

Front Microbiol 2021 6;12:707609. Epub 2021 Aug 6.

Department of Molecular Genetics, University of Groningen, Groningen, Netherlands.

The gray mold caused by the phytopathogen presents a threat to global food security. For the biological regulation of several plant diseases, species have been extensively studied. In this work, we explore the ability of a bacterial strain, BH5, that was isolated from tomato rhizosphere soil, to control the fungal pathogen . Strain BH5 showed a strong antifungal activity against . A compound was isolated and identified as a cyclic lipopeptide of the fengycin family by high-performance liquid chromatography and tandem mass spectrometry (ESI-MS/MS) that we named fengycin H. The fengycin H-treated hyphae of displayed stronger red fluorescence than the control, which is clearly indicating that fengycin H triggered the hyphal cell membrane defects. Moreover, root inoculation of tomato seedlings with BH5 effectively promoted the growth of tomato plants. Transcription analysis revealed that both BH5 and fengycin H stimulate induced systemic resistance of tomato plants the jasmonic acid signaling pathway and provide a strong biocontrol effect . Therefore, the strain BH5 and fengycin H are very promising candidates for biological control of and the associated gray mold.
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http://dx.doi.org/10.3389/fmicb.2021.707609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8441496PMC
August 2021

Light-harvesting complex stress-related proteins play crucial roles in the acclimation of Physcomitrella patens under fluctuating light conditions.

Photosynth Res 2021 Sep 1. Epub 2021 Sep 1.

CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

Photosynthetic organisms have evolved photoprotective mechanisms to acclimate to light intensity fluctuations in their natural growth environments. Photosystem (PS) II subunit S (PsbS) and light-harvesting complex (LHC) stress-related proteins (LhcSR) are essential for triggering photoprotection in vascular plants and green algae, respectively. The activity of both proteins is strongly enhanced in the moss Physcomitrella patens under high-light conditions. However, their role in regulating photosynthesis acclimation in P. patens under fluctuating light (FL) conditions is still unknown. Here, we compare the responses of wild-type (WT) P. patens and mutants lacking PsbS (psbs KO) or LhcSR1 and 2 (lhcsr KO) to FL conditions in which the low-light phases were periodically interrupted with high-light pulses. lhcsr KO mutant showed a strong reduction in growth with respect to WT and psbs KO under FL conditions. The lack of LhcSR not only decreased the level of non-photochemical quenching, resulting in an over-reduced plastoquinone pool, but also significantly increased the PSI acceptor limitation values with respect to WT and psbs KO under FL conditions. Moreover, in lhcsr KO mutant, the abundance of PSI core and PSI-LHCI complex decreased greatly under FL conditions compared with the WT and psbs KO. We proposed that LhcSR in P. patens play a crucial role in moss acclimation to dynamic light changes.
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http://dx.doi.org/10.1007/s11120-021-00874-8DOI Listing
September 2021

Effects of a Chlorogenic Acid-Containing Herbal Medicine (LAS) on Colon Cancer.

Evid Based Complement Alternat Med 2021 20;2021:9923467. Epub 2021 Aug 20.

Oncology Department, Chengdu Fuxing Hospital, Chengdu, China.

Background: Plant polyphenols, which contain phenolic acids such as chlorogenic acid (CGA), can be used for the treatment of gastrointestinal cancer and have gained increasing attention in recent years. In this study, we explored a novel CGA-containing herbal medicine named LAS, which was extracted from Thunb., L., and D.Don.

Methods: CGA in LAS was analyzed using high-performance liquid chromatography (HPLC). The biological functions and molecular mechanisms of LAS were investigated in colon cancer cell lines (HCT116, HCT15, and CT26), a normal colon cell line (NCM460), and a CT26 xenograft model. To assess safety, hematological toxicity and pathology of the liver, kidney, and lung were evaluated.

Results: LAS suppressed HCT116, HCT15, and CT26 colon cancer progression by inhibiting proliferation capacity, promoting cell apoptosis, and suppressing cell migration both in vitro and in vivo. Investigation into the underlying molecular mechanism indicated that LAS suppressed the activation of receptor tyrosine kinase- (RTK-) MEK-ERK and NF-B pathways. With regard to safety, slight interstitial vascular congestion in the lung was observed, but no severe pathological or hematological toxicity was detected.

Conclusions: We found that LAS suppressed the progression of colon cancer via the RTK-MEK-ERK and NF-B pathways, with no severe toxicity observed. Therefore, LAS has the potential to be used as a supplementary herbal medicine for the treatment of colon cancer.
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http://dx.doi.org/10.1155/2021/9923467DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403046PMC
August 2021

Metastasis suppressor 1 interacts with α-actinin 4 to affect its localization and regulate formation of membrane ruffling.

Cytoskeleton (Hoboken) 2021 Jul 2;78(7):337-348. Epub 2021 Sep 2.

The Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, China.

Membrane ruffling plays an important role in the directed cell migration and escape of tumor cells from the monolayer. Metastasis suppressor 1 (MTSS1), also known as missing in metastasis, has been implicated in cell morphology, motility, metastasis, and development. Here, the dynamic interaction proteins associated with MTSS1 and involved in membrane ruffling were determined by cross-linking and mass spectrometry analysis. We identified α-actinin 4 (ACTN4) as an interacting protein and confirmed a direct interaction between MTSS1 and ACTN4. Moreover, co-expression of MTSS1 in fibroblasts recruited cytoplasmic ACTN4 to the cell periphery, at which point ruffling became thick and rigid. In MCF-7 cells, MTSS1 knockdown did not show an obvious effect on the cell shape or the distribution of endogenous ACTN4; however, ACTN4 overexpression transformed cell morphology from an epidermal- to a fibroblast-like shape, and further MTSS1 depletion significantly increased the ratio of fibroblast cells exhibiting prominent ruffling. Furthermore, biochemical data suggested that MTSS1 cross-linking with ACTN4 induced the formation of actin fiber bundles into more organized structures in vitro. These data indicated that MTSS1 might recruit cytoplasmic ACTN4 to the cell periphery and regulate cytoskeleton dynamics to restrict its performance in membrane ruffling.
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http://dx.doi.org/10.1002/cm.21686DOI Listing
July 2021

Rapid Determination of 21 Chinese Domestically Registered Pesticides in Ginseng Using Cleanup Based on Zirconium-Oxide-Modified Silica and Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry.

J Anal Methods Chem 2021 13;2021:5516563. Epub 2021 Aug 13.

College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin, China.

In this study, an analytical method was developed for the rapid determination of 21 pesticides used in ginseng cultivation. All pesticides covered by this method have been registered by 2020 in China for use on ginseng. The extracts were cleaned up using zirconium-oxide-modified silica (Z-Sep) and primary secondary amine (PSA). The combination of Z-Sep and PSA provided good recovery for all analytes and the cleanest matrix background out of a number of PSA-based sorbent combinations, as indicated by high-performance liquid chromatography (HPLC) and gas chromatography (GC). Instrumental analysis was completed in 5 min using the ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The linearity ( > 0.99) for all analytes was satisfactory over the calibration range of 0.002-0.1 g mL. Intraday recoveries ( = 5) at ginseng-spiked levels of 0.02, 0.05, 0.1, and 1 mg kg ranged between 72% and 119%, with the corresponding relative standard deviations (RSDs), were less than 19%, while the interday recoveries ( = 15) ranged between 77% and 103%, and RSDs were less than 22%. Limits of quantitation (LOQs) ranged between 0.02 and 0.05 mg kg for all 21 pesticides. This is a seminal study using Z-Sep for the efficient cleanup of ginseng samples, and it could present a practical method for future monitoring of pesticide residues in ginseng produced in China.
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http://dx.doi.org/10.1155/2021/5516563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8378960PMC
August 2021

Stimulation of pyrolytic carbon materials as electron shuttles on the anaerobic transformation of recalcitrant organic pollutants: A review.

Sci Total Environ 2021 Aug 17;801:149696. Epub 2021 Aug 17.

School of Hydraulic Engineering, Changsha University of Science & Technology, Changsha 410114, PR China; Key Laboratory of Dongting Lake Aquatic Eco-Environmental Control and Restoration of Hunan Province, Changsha 410114, PR China.

Pyrolytic carbon materials (PCMs) with various surface functionalities are widely used as environmentally friendly and cost-efficient adsorbents for the removal of organic and inorganic pollutants. Recent studies have illustrated that PCMs as electron shuttles (ESs) could also show excellent performances in promoting the anaerobic transformation of recalcitrant organic pollutants (ROPs). Numerous studies have demonstrated the excellent electron-shuttle capability (ESC) of PCMs to stimulate the anaerobic reductive transformation of ROPs. However, there is a lack of consistent understanding of the mechanism of ESC formation in PCMs and the stimulation mechanism for ROPs anaerobic transformation. To gain a more comprehensive understanding of the latest developments in the study of PCMs as ESs for ROPs anaerobic transformation, this review summarizes the formation mechanism, influencing factors, and stimulation mechanisms of ESC. ESC benefits from redox functional groups (quinone and phenol groups), persistent free radicals (PFRs), redox-active metal ions, conductive graphene phase, and porous nature of their surface. The factors influencing ESC include the highest treatment temperature (HTT), feedstocks, modification methods, and environmental conditions, of which, the HTT is the key factor. PCMs promote the reductive transformation of ROPs under anaerobic conditions via abiotic and biotic pathways. Eventually, the prospects for the ROPs anaerobic transformation enhanced by PCMs are proposed.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149696DOI Listing
August 2021

The role of genetics in neurodegenerative dementia: a large cohort study in South China.

NPJ Genom Med 2021 Aug 13;6(1):69. Epub 2021 Aug 13.

Department of Neurology, Xiangya Hospital, Central South University, Changsha, China.

Neurodegenerative dementias are a group of diseases with highly heterogeneous pathology and complicated etiology. There exist potential genetic component overlaps between different neurodegenerative dementias. Here, 1795 patients with neurodegenerative dementias from South China were enrolled, including 1592 with Alzheimer's disease (AD), 110 with frontotemporal dementia (FTD), and 93 with dementia with Lewy bodies (DLB). Genes targeted sequencing analysis were performed. According to the American College of Medical Genetics (ACMG) guidelines, 39 pathogenic/likely pathogenic (P/LP) variants were identified in 47 unrelated patients in 14 different genes, including PSEN1, PSEN2, APP, MAPT, GRN, CHCHD10, TBK1, VCP, HTRA1, OPTN, SQSTM1, SIGMAR1, and abnormal repeat expansions in C9orf72 and HTT. Overall, 33.3% (13/39) of the variants were novel, the identified P/LP variants were seen in 2.2% (35/1592) and 10.9% (12/110) of AD and FTD cases, respectively. The overall molecular diagnostic rate was 2.6%. Among them, PSEN1 was the most frequently mutated gene (46.8%, 22/47), followed by PSEN2 and APP. Additionally, the age at onset of patients with P/LP variants (51.4 years), ranging from 30 to 83 years, was ~10 years earlier than those without P/LP variants (p < 0.05). This study sheds insight into the genetic spectrum and clinical manifestations of neurodegenerative dementias in South China, further expands the existing repertoire of P/LP variants involved in known dementia-associated genes. It provides a new perspective for basic research on genetic pathogenesis and novel guiding for clinical practice of neurodegenerative dementia.
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http://dx.doi.org/10.1038/s41525-021-00235-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363644PMC
August 2021

Mutations in , and are not associated with Alzheimer's disease in a Chinese population: a case-control study.

Neural Regen Res 2022 Mar;17(3):682-689

Department of Neurology, Xiangya Hospital; National Clinical Research Center for Geriatric Disorders; Engineering Research Center of Hunan Province in Cognitive Impairment Disorders; Hunan International Scientific and Technological Cooperation Base of Neurodegenerative and Neurogenetic Diseases; Key Laboratory of Hunan Province in Neurodegenerative Disorders, Central South University, Changsha, Hunan Province, China.

SNCA, GBA, and VPS35 are three common genes associated with Parkinson's disease. Previous studies have shown that these three genes may be associated with Alzheimer's disease (AD). However, it is unclear whether these genes increase the risk of AD in Chinese populations. In this study, we used a targeted gene sequencing panel to screen all the exon regions and the nearby sequences of GBA, SNCA, and VPS35 in a cohort including 721 AD patients and 365 healthy controls from China. The results revealed that neither common variants nor rare variants of these three genes were associated with AD in a Chinese population. These findings suggest that the mutations in GBA, SNCA, and VPS35 are not likely to play an important role in the genetic susceptibility to AD in Chinese populations. The study was approved by the Ethics Committee of Xiangya Hospital, Central South University, China on March 9, 2016 (approval No. 201603198).
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http://dx.doi.org/10.4103/1673-5374.321000DOI Listing
March 2022

Hypoxia facilitates the proliferation of colorectal cancer cells by inducing cancer-associated fibroblast-derived IL6.

Neoplasma 2021 Sep 11;68(5):1015-1022. Epub 2021 Aug 11.

Digestive Endoscopy Center, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Colorectal cancer (CRC) is the most common malignancy worldwide, and its underlying molecular mechanisms remain largely unexplored. Accumulating evidences indicate cancer-associated fibroblasts (CAFs), abundant stromal cell population in the tumor microenvironment, play a key role in tumor development. Herein, we have successfully isolated CAFs and paired normal fibroblasts (NFs) from colorectal cancer tissues (n=10). By using a multiplex cytokine profiling assay, we have identified IL-6 as a major cytokine released by CAFs. Co-culturing of CAFs with CRC cell lines HCT116 or SW480 increases IL-6 release, and the secretion by CAFs can be further enhanced under hypoxia. By using the CCK-8 assay, we have found that HCT116 or SW480 cells treated with culture medium from CAFs, IL-6, or hypoxia showed a significant cell growth compared to control cells (p<0.01). Mechanistically, we have found that hypoxia could enhance the effect of the IL-6/STAT3 signaling on CRC cells, in part, through HIF-1a targeting PKM2. In conclusion, our data clearly proposes the interconnected mechanisms for constitutive activation of STAT3 signal by CAFs-derived IL-6 under hypoxia in colorectal cancer. The pharmacological inhibition of STAT3, PKM2, or HIF-1α can significantly reduce the oncogenic effect of IL-6, providing a potential therapeutic target for CRC patients.
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http://dx.doi.org/10.4149/neo_2021_210308N296DOI Listing
September 2021

Bicyclol Alleviates Signs of BDL-Induced Cholestasis by Regulating Bile Acids and Autophagy-Mediated HMGB1/p62/Nrf2 Pathway.

Front Pharmacol 2021 21;12:686502. Epub 2021 Jul 21.

Department of Gastroenterology and Hepatology, Tianjin Medical University General Hospital, Tianjin Institute of Digestive Disease, Tianjin Key Laboratory of Digestive Diseases, Tianjin, China.

Cholestasis is a liver disease characterized by the accumulation of toxic bile salts, bilirubin, and cholesterol, resulting in hepatocellular damage. Recent findings have revealed several key steps of cholestasis liver injury including the toxicity of bile acids and accumulation of proinflammatory mediator. In this study, we investigated the protective effect of bicyclol in cholestasis caused by bile duct ligation (BDL), as well as relevant mechanisms. Bicyclol attenuated liver damage in BDL mice by increasing the levels of hydrophilic bile acid such as α-MCA and β-MCA, regulating bile acid-related pathways and improving histopathological indexes. High-mobility group box 1 (HMGB1) is an extracellular damage-associated molecular pattern molecule which can be used as biomarkers of cells and host defense. Bicyclol treatment decreased extracellular release of HMGB1. In addition, HMGB1 is also involved in regulating autophagy in response to oxidative stress. Bicyclol promoted the lipidation of LC3 (microtubule-associated protein 1 light chain 3)-Ⅱ to activate autophagy. The nuclear factor, E2-related factor 2 (Nrf2) and its antioxidant downstream genes were also activated. Our results indicate that bicyclol is a promising therapeutic strategy for cholestasis by regulating the bile acids and autophagy-mediated HMGB1/p62/Nrf2 pathway.
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http://dx.doi.org/10.3389/fphar.2021.686502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8334002PMC
July 2021

The signaling role of extracellular ATP in co-culture of Shiraia sp. S9 and Pseudomonas fulva SB1 for enhancing hypocrellin A production.

Microb Cell Fact 2021 Jul 23;20(1):144. Epub 2021 Jul 23.

College of Pharmaceutical Sciences, Soochow University, Suzhou, 215123, China.

Background: Adenosine 5'-triphosphate (ATP) plays both a central role as an intracellular energy source, and a crucial extracellular signaling role in diverse physiological processes of animals and plants. However, there are less reports concerning the signaling role of microbial extracellular ATP (eATP). Hypocrellins are effective anticancer photodynamic therapy (PDT) agents from bambusicolous Shiraia fungi. The co-culture of Shiraia sp. S9 and a bacterium Pseudomonas fulva SB1 isolated from Shiraia fruiting bodies was established for enhanced hypocrellin A (HA) production. The signaling roles of eATP to mediate hypocrellin biosynthesis were investigated in the co-culture.

Results: The co-culture induced release of eATP at 378 nM to the medium around 4 h. The eATP release was interdependent on cytosolic Ca concentration and reactive oxygen species (ROS) production, respectively. The eATP production could be suppressed by the Ca chelator EGTA or abolished by the channel blocker La, ROS scavenger vitamin C and NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI). The bacterium-induced HO production was strongly inhibited by reactive blue (RB), a specific inhibitor of membrane purinoceptors, but dependent on the induced Ca influx in the co-culture. On the other hand, the application of exogenous ATP (exATP) at 10-300 µM to Shiraia cultures also promoted fungal conidiation and HA production, both of which were blocked effectively by the purinoceptor inhibitors pyridoxalphosphate-6-azophenyl-2', 4'-disulfonic acid (PPADS) and RB, and ATP hydrolase apyrase. Both the induced expression of HA biosynthetic genes and HA accumulation were inhibited significantly under the blocking of the eATP or Ca signaling, and the scavenge of ROS in the co-culture.

Conclusions: Our results indicate that eATP release is an early event during the intimate bacterial-fungal interaction and eATP plays a signaling role in the bacterial elicitation on fungal metabolites. Ca and ROS are closely linked for activation of the induced ATP release and its signal transduction. This is the first report on eATP production in the fungal-bacterial co-culture and its involvement in the induced biosynthesis of fungal metabolites.
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http://dx.doi.org/10.1186/s12934-021-01637-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305905PMC
July 2021

Application of computer tongue image analysis technology in the diagnosis of NAFLD.

Comput Biol Med 2021 08 1;135:104622. Epub 2021 Jul 1.

Basic Medical College, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai, 201203, China. Electronic address:

Nonalcoholic fatty liver disease (NAFLD), a leading cause of chronic hepatic disease, can progress to liver fibrosis, cirrhosis, and hepatocellular carcinoma. Therefore, it is extremely important to explore early diagnosis and screening methods. In this study, we developed models based on computer tongue image analysis technology to observe the tongue characteristics of 1778 participants (831 cases of NAFLD and 947 cases of non-NAFLD). Combining quantitative tongue image features, basic information, and serological indexes, including the hepatic steatosis index (HSI) and fatty liver index (FLI), we utilized machine learning methods, including Logistic Regression, Support Vector Machine (SVM), Random Forest (RF), Gradient Boosting Decision Tree (GBDT), Adaptive Boosting Algorithm (AdaBoost), Naïve Bayes, and Neural Network for NAFLD diagnosis. The best fusion model for diagnosing NAFLD by Logistic Regression, which contained the tongue image parameters, waist circumference, BMI, GGT, TG, and ALT/AST, achieved an AUC of 0.897 (95% CI, 0.882-0.911), an accuracy of 81.70% with a sensitivity of 77.62% and a specificity of 85.22%; in addition, the positive likelihood ratio and negative likelihood ratio were 5.25 and 0.26, respectively. The application of computer intelligent tongue diagnosis technology can improve the accuracy of NAFLD diagnosis and may provide a convenient technical reference for the establishment of early screening methods for NAFLD, which is worth further research and verification.
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http://dx.doi.org/10.1016/j.compbiomed.2021.104622DOI Listing
August 2021

Reduced graphene oxide modified Z-scheme AgI/BiMoO heterojunctions with boosted photocatalytic activity for water treatment originated from the efficient charge pairs partition and migration.

Environ Sci Pollut Res Int 2021 Jul 7. Epub 2021 Jul 7.

Research Center of Resource Environment and Urban Planning, Changsha University of Science and Technology, Changsha, 410114, People's Republic of China.

In order to enhance degradation of harmful organic pollutants like Rhodamine B (RhB) dye under visible-light irradiation (λ >420 nm), a silver iodide/reduced graphene oxide/bismuth molybdate (AgI/rGO/BiMoO) Z-scheme heterojunction photocatalyst was synthesized by a solvothermal process combined with an in-situ precipitation technique. The AgI (15 wt.%)/rGO/BiMoO (AGBMO-15) photocatalyst with a dosage of 0.5 g/L exhibited the highest photocatalytic activity with 98.0% RhB removal under an initial concentration of 10 mg/L within 30 min. This removal rate was approximately 65.8%, 57.7%, and 72.7% higher than that for a rGO/BiMoO (GBMO) binary composite, pure AgI powder, and pristine BiMoO nanoplates, respectively. The novel photocatalyst achieved approximately three times higher photocatalytic degradation within a shorter period of visible-light irradiation than pure BiMoO. Through photoluminescence analysis and trapping experiments, this outstanding performance was attributed to the efficient separation of photogenerated electron-hole pairs owing to an internal electric field at the contact interface of AgI and BiMoO, which generated more superoxide radical anions (•O) as primary reactive species to promote RhB degradation. Meanwhile, the rGO participated in the capture of visible-light and played a role of solid electronic medium at the AgI/BiMoO interface, which realized an effective Z-scheme electron transfer path, avoided the self oxidation of photocatalyst and prolonged the carrier life. Furthermore, the AGBMO-15 photocatalyst exhibited excellent photocatalytic degradation stability, maintaining an RhB removal rate of 96.2% after four cycles of reuse. Due to its simplicity, reusability, and controllability, the proposed photocatalyst has excellent application potential for the environmental remediation of wastewater.
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http://dx.doi.org/10.1007/s11356-021-15180-yDOI Listing
July 2021

The role of frontotemporal dementia associated genes in patients with Alzheimer's disease.

Neurobiol Aging 2021 May 30. Epub 2021 May 30.

Department of Neurology, Xiangya Hospital, Central South University, Changsha, China; National Clinical Research Center for Geriatric Disorders, Central South University, Changsha, China; Engineering Research Center of Hunan Province in Cognitive Impairment Disorders, Central South University, Changsha, China; Hunan International Scientific and Technological Cooperation Base of Neurodegenerative and Neurogenetic Diseases, Changsha, China; Key Laboratory of Hunan Province in Neurodegenerative Disorders, Central South University, Changsha, China. Electronic address:

Alzheimer's disease (AD) and frontotemporal dementia (FTD) overlap clinically and pathologically. However, the role of FTD-associated genes in patients with AD remained unclear. To explore the relationship between FTD-associated genes and AD risk, we investigated 14 FTD-associated genes via targeted next-generation sequencing panel or whole-genome sequencing in a total of 721 AD patients and 1391 controls. Common variant-based association analysis and gene-based association test of rare variants were performed by PLINK 1.9 and Sequence Kernel Association Test-Optimal (SKAT-O test) respectively. As a result, 2 common variants, UBQLN1 rs1044175 (p value = 2.76 × 10) and MAPT rs2258689 (p value = 5.71 × 10), differed significantly between AD patients and controls. Additionally, gene-based analysis aggregating rare variants demonstrated that HNRNPA1 reached statistical significance in the SKAT-O test (p value = 2.24 × 10). Protein-protein interaction analysis showed that UBQLN1, MAPT, and HNRNPA1 interacted with proteins encoded by well-recognized AD-associated genes. Our study indicated that UBQLN1, MAPT, and HNRNPA1 are implicated in the pathogenesis of AD in the mainland Chinese population.
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http://dx.doi.org/10.1016/j.neurobiolaging.2021.05.016DOI Listing
May 2021

Aggregation-induced phosphorescence sensitization in two heptanuclear and decanuclear gold-silver sandwich clusters.

Chem Sci 2020 Oct 30;12(2):702-708. Epub 2020 Oct 30.

College of Chemistry and Materials Science, Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications, Jinan University Guangzhou 510632 P. R. China

The strategy of aggregation-induced emission enhancement (AIEE) has been proven to be efficient in wide areas and has recently been adopted in the field of metal nanoclusters. However, the relationship between atomically precise clusters and AIEE is still unclear. Herein, we have successfully obtained two few-atom heterometallic gold-silver hepta-/decanuclear clusters, denoted and , and determined their structures by X-ray diffraction and mass spectrometry. The nature of the Au⋯Ag interactions thereof is demonstrated through energy decomposition analysis to be far-beyond typical closed-shell metal-metal interaction dominated by dispersion interaction. Furthermore, a positive correlation has been established between the particle size of the nanoaggregates and the photoluminescence quantum yield for , manifesting AIEE control upon varying the stoichiometric ratio of Au : Ag in atomically-precise clusters.
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http://dx.doi.org/10.1039/d0sc05095dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179010PMC
October 2020

Pressure-induced phosphorescence enhancement and piezochromism of a carbazole-based cyclic trinuclear Cu(i) complex.

Chem Sci 2021 Jan 28;12(12):4425-4431. Epub 2021 Jan 28.

College of Chemistry and Materials Science, Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications, Jinan University Guangzhou Guangdong 510632 People's Republic of China

Interest in piezochromic luminescence has increased in recent decades, even though it is mostly limited to pure organic compounds and fluorescence. In this work, a CuPz (, Pz: pyrazolate) cyclic trinuclear complex (CTC) with two different crystalline polymorphs, namely and , was synthesized. The CTC consists of two functional moieties: carbazole () chromophore and units. In crystals of , discrete --- stacking was found, showing abnormal pressure-induced phosphorescence enhancement (PIPE), which was 12 times stronger at 2.23 GPa compared to under ambient conditions. This novel observation is ascribed to cooperation between heavy-atom effects (, from Cu atoms) and metal-ligand charge-transfer promotion. The infinite π-π stacking of motifs was observed in and it exhibited good piezochromism as the pressure increased. This work demonstrates a new concept in the design of piezochromic materials to achieve PIPE combining organic chromophores and metal-organic phosphorescence emitters.
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http://dx.doi.org/10.1039/d0sc07058kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179561PMC
January 2021
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