Publications by authors named "Lu Zhang"

3,607 Publications

  • Page 1 of 1

Analysis of the prevalence, risk factors, and clinical characteristics of osteoporosis in patients with essential hypertension.

BMC Endocr Disord 2022 Jun 27;22(1):165. Epub 2022 Jun 27.

Changchun University of Chinese Medicine, Changchun, Jilin, China.

Background: The present study investigated the prevalence of osteoporosis (OP) among patients with essential hypertension (EH) in the Changchun community and analysed the correlation between EH and OP.

Methods: The study included 425 subjects with EH and 425 age- and sex-matched healthy controls. Bone mineral density (BMD) and serum creatinine (CR) levels were measured, and the subjects' current EH and OP statuses were surveyed to analyse the correlation between EH and OP.

Results: The EH group exhibited lower BMD and a higher rate of having OP than the control group, and this difference was statistically significant (p < 0.05). A significant sex difference in the BMD T-score was observed among the subjects (male: - 1.19 ± 1.55, female: - 1.70 ± 1.34). In both the EH group and the control group, the rate of having OP in females was greater than that in males. However, the OP prevalence among subjects with EH varied significantly by age, body weight, fracture history, nocturnal urination frequency, depression and anxiety status, duration of hypertension, and antihypertensive medication use (p < 0.05). Two-way analysis of variance suggested an effect of the interaction between different EH statuses and bone mass conditions on the serum CR values (F = 3.584, p = 0.028, bias η = 0.008).

Conclusions: The prevalence of OP and low BMD were significantly higher among subjects with EH than among healthy controls. Additionally, the findings indicate that age, weight, fracture history, nocturnal urination frequency, depression and anxiety, duration of hypertension and antihypertensive drug use may be correlated to having OP in EH subjects, requiring further studies. Moreover, serum CR levels in subjects with different bone mass profiles were strongly influenced by the presence or absence of EH, and the serum CR levels differed significantly with the interaction of these two factors.
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http://dx.doi.org/10.1186/s12902-022-01080-wDOI Listing
June 2022

Toxic metals in rice-fish co-culture systems and human health.

Ecotoxicol Environ Saf 2022 Jun 24;241:113797. Epub 2022 Jun 24.

Fisheries Institute, Sichuan Academy of Agricultural Sciences, Chengdu 611731, Sichuan, China. Electronic address:

The threat of contamination with toxic metals (TMs) to food security and human health has become a high priority in recent decades. Hence, countless studies have investigated the safe cultivation of rice and fish, respectively, as the main food crop and protein source worldwide. For the present study, a literature search of the PubMed, Web of Science, ScienceDirect, and China National Knowledge Infrastructure databases identified 11 studies that met the inclusion criteria and provided sufficient data to assess the relationship between TM contamination of rice, fish, and shrimp products from rice-fish co-culture systems and carcinogenic risk (CR) and non-carcinogenic risk (non-CR) to humans. The result showed that consumption of Monopterus albus and rice contaminated with a single TM had a slight non-CR, which is synergistically increased by multiple TMs. Consumption of Procambarus clarkii posed no non-CR to humans. The CR of all studies ranged from 1.77 × 10 to 5.65 × 10, and less than 1 × 10, indicating that under current food safety guidelines, rice and fish produced by rice-fish co-culture systems pose no CR. Rice-fish co-culture systems can greatly reduce the CR and non-CR of TMs to humans.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113797DOI Listing
June 2022

Crosstalk between lysine methyltransferase Smyd2 and TGF-β-Smad3 signaling promotes renal fibrosis in autosomal dominant polycystic kidney disease.

Am J Physiol Renal Physiol 2022 Jun 27. Epub 2022 Jun 27.

Department of Internal Medicine, Mayo Clinic, Rochester, MN, United States.

Autosomal dominant polycystic kidney disease (ADPKD) is an inherited genetic disorder, which is caused by mutations of PKD1 or PKD2 gene and is characterized by renal fluid-filled cyst formation and interstitial fibrosis. PKD1 gene mutation results in the upregulation of SET and MYND domain-containing lysine methyltransferase 2 (SMYD2) in Pkd1 mutant mouse and ADPKD patient kidneys. However, the role and mechanism of Smyd2 in the regulation of renal fibrosis in ADPKD remains elusive. In this study, we show that: 1) the expression of Smyd2 can be regulated by TGF-β-Smad3 in normal rat kidney 49F (NRK-49F) cells and mouse fibroblast NIH3T3 cells; 2) knockdown of Smyd2 and inhibition of Smyd2 with its specific inhibitor, AZ505, decreases TGF-β-induced expression of α-smooth muscle actin (α-SMA), fibronectin, collagens 1 and 3 and plasminogen activator inhibitor-1( PAI1) in NRK-49F cells; 3) Smyd2 regulates the transcription of fibrotic marker genes through binding on the promoters of those genes or through methylating histone H3 to indirectly regulate the expression of those genes; and 4) knockout and inhibition of Smyd2 significantly decreases renal fibrosis in Pkd1 knockout mice, supporting that targeting Smyd2 can not only delay cyst growth but also attenuate renal fibrosis in ADPKD. This study identifies a crosstalk between TGF-β signaling and Smyd2 in the regulation of fibrotic gene transcription and the activation of fibroblasts in cystic kidneys, suggesting that targeting Smyd2 with AZ505 is a potential therapeutic strategy for ADPKD treatment.
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http://dx.doi.org/10.1152/ajprenal.00452.2021DOI Listing
June 2022

Vertical "V-Y" Advancement Lifting Based on Fat Compartments: A New Approach for Lower Eyelid and Midcheek Rejuvenation.

Plast Reconstr Surg 2022 Jun 28. Epub 2022 Jun 28.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine; Shanghai, China.

Background: Lower blepharoplasty continues to improve over time. With discovery of the numerous fat compartments of the face, surgeons noticed that the appearance of the lower eyelid area through aging could be improved through midface lifting. This study introduces a vertical "V-Y" advancement lifting technique, using the concept of fat compartments for lower lid and midcheek rejuvenation.

Methods: Eighty female patients were categorized into four age groups at 20 patients each from 20- to 59-year-olds. The average measurement for the lower lid length in each group was recorded. Additionally, 318 patients underwent lower eyelid blepharoplasty combined with fat compartments advancement lifting for lid-cheek area rejuvenation from April 2015 to May 2018 were included in this study. The postoperative effect was evaluated based on pre- and postoperative photographs, lower lid length measurements, and three-dimensional facial analyses.

Results: The lower lid lengths gradually increased with age, and the greatest increase occurred in the 30- to 39-year-old group. 318 Asian female patients underwent the surgical procedure, with the patients' age ranging from 35 to 58 years old. Measurements of the lower lid length showed an average reduction of 0.31cm 6 months post-surgery. Three-dimensional facial analysis also indicated that the patient's midcheek volume increased at varying degrees post-surgery.

Conclusions: Lower eyelid blepharoplasty combined with vertical "V-Y" advancement lifting makes it possible to restore facial fat compartments. This procedure improves projection in the midcheek without any kinds of fillers, thus achieving a more youthful appearance of the lower lid and lid-cheek contour in middle-aged women.
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http://dx.doi.org/10.1097/PRS.0000000000009461DOI Listing
June 2022

Impact of in vitro Fertilization-Embryo Transfer on Mother-to-Infant Transmission in Women with Chronic HBV Infection.

Liver Int 2022 Jun 27. Epub 2022 Jun 27.

Department of Hepatology Division 2, Beijing Ditan Hospital, Capital Medical University, Beijing, China.

Background & Aims: In vitro fertilization-embryo transfer (IVF-ET) may increase the risk of mother-to-child transmission (MTCT) of hepatitis B virus (HBV). The purpose of this study was to investigate the impact and safety of IVF-ET on MTCT in women with chronic HBV infection (CHB).

Methods: The data of 298 women who got pregnant by IVF-ET and their 375 children was collected retrospectively. Mothers were divided into CHB group (n=224) and control group (HBsAg negative, n=74). After birth, newborns were routinely vaccinated with hepatitis B vaccine, and infants in CHB group were injected with hepatitis B immunoglobulin within 2 hours after birth. Demographic information, clinical data and laboratory test results was collected. The primary outcome measures was the MTCT rate of HBV, and the secondary outcome measures was the safety of mother and infant.

Results: There was no case of HBV MTCT in all 282 newborns born to CHB group and 93 neonates born to control group. Of the 2 groups, the birth weight (3056.74±601.65 vs 2926.24±704.86, P = 0.083), length (49.22±1.97 vs 48.74±3.09, P = 0.167), 5-minute Apgar score (9.97±0.21 vs 9.90±0.51, P = 0.212), days of pregnancy (265.70±12.73 vs 262.02±17.50, P = 0.064), neonatal malformation rate (0.71% vs 0, P = 1.000) were similar. Two cases of neonatal malformation occurred in CHB group. The incidences of pregnancy and childbirth complications were similar between the two groups.

Conclusion: IVF-ET does not increase the risk of MTCT in women with chronic HBV infection, and it is safe for mothers and infants.
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http://dx.doi.org/10.1111/liv.15349DOI Listing
June 2022

Analysis of the HVTN 702 Phase 2b-3 HIV-1 vaccine trial in South Africa assessing RV144 antibody and T-cell correlates of HIV-1 acquisition risk.

J Infect Dis 2022 Jun 27. Epub 2022 Jun 27.

Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, Washington 98109, USA.

Background: The ALVAC/gp120 + MF59 vaccines in the HVTN 702 efficacy trial did not prevent HIV-1 acquisition. Vaccine-matched immunological endpoints that were correlates of HIV-1 acquisition risk in RV144 were measured in HVTN 702 and evaluated as correlates of HIV-1 acquisition.

Methods: Among 1893 HVTN 702 female vaccinees, 60 HIV-1-seropositive cases and 60 matched seronegative non-cases were sampled. HIV-specific CD4+ T-cell and binding antibody responses were measured 2 weeks post-fourth and fifth immunizations. Cox proportional hazards models assessed pre-specified responses as predictors of HIV-1 acquisition.

Results: The HVTN 702 Env-specific CD4+ T-cell response rate was significantly higher than in RV144 (63% vs. 40%, P = 0.03) with significantly lower IgG binding antibody response rate and magnitude to 1086.C V1V2 (67% vs. 100%, P< 0.001; Pmag< 0.001). Although no significant univariate associations were observed between any T-cell or binding antibody response and HIV-1 acquisition, significant interactions were observed (multiplicity-adjusted P<=0.03). Among vaccinees with high IgG A244 V1V2 binding antibody responses, vaccine-matched CD4+ T-cell endpoints associated with decreased HIV-1 acquisition (estimated hazard ratios = 0.40-0.49 per 1-SD increase in CD4+ T-cell endpoint).

Conclusions: HVTN 702 and RV144 had distinct immunogenicity profiles. However, both identified significant correlations (univariate or interaction) for IgG V1V2 and polyfunctional CD4+ T-cells with HIV-1 acquisition.
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http://dx.doi.org/10.1093/infdis/jiac260DOI Listing
June 2022

Expression and Clinical Significance of the m6A RNA-Binding Proteins in Peripheral Blood Mononuclear Cells From New-Onset Ankylosing Spondylitis.

Front Med (Lausanne) 2022 9;9:922219. Epub 2022 Jun 9.

Department of Clinical Laboratory, The First Affiliated Hospital of Nanchang University, Nanchang, China.

This study has focused on determining the association of m6A methyltransferase [methyltransferase-like 3 (METTL3), methyltransferase-like 14 (METTL14), and Wilms tumor 1-associating protein (WTAP)], demethylase [fat mass and obesity-associated protein (FTO) and alkylation repair homolog protein 5 (ALKBH5)], RNA-binding proteins [YT521-B homology domains 2 (YTHDF2)], and ankylosing spondylitis (AS). A total of 154 specimens, containing 79 patients with new-onset AS and 75 healthy controls (HCs), participated in the study. The mRNA expressions of these m6A methyltransferase, demethylase, and RNA-binding protein in peripheral blood mononuclear cells (PBMCs) were detected by quantitative real-time PCR (qRT-PCR). The data showed that the mRNA expressions of and in PBMC from patients with new-onset AS were significantly decreased, and there was a positive correlation between RNA-binding proteins () and demethylase () in patients with new-onset AS. Logistic regression analysis demonstrated that the expression of mRNA in PBMC is a risk factor of AS. Receiver operating characteristic (ROC) analysis of the area under the curve (AUC) for mRNA in new-onset AS and HC was 0.692, with a cutoff value of <0.8724, a sensitivity of 67%, and a specificity of 63%. Moreover, we constructed a novel predictive model based on a combination of mRNA and systemic immune-inflammation index (SII) for AS diagnosis (AUC = 0.865, sensitivity = 79.45%, specificity = 84.00%), and the predictive model correlated with the activity and severity of AS. This study indicates that the mRNA expression of in PBMC may be involved in AS pathogenesis and a predictive model based on a combination of mRNA and SII acts as a marker for diagnosis and progression of diseases.
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http://dx.doi.org/10.3389/fmed.2022.922219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218531PMC
June 2022

Risk factors for midcourse correction during treatment of first series of aligners with Invisalign.

Am J Orthod Dentofacial Orthop 2022 Jun 23. Epub 2022 Jun 23.

Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China. Electronic address:

Introduction: This study aimed to analyze risk factors for midcourse correction (MC) during the first series of aligners in treatments with Invisalign (Align Technology, Santa Clara, Calif).

Methods: Three hundred and fourteen patients treated with Invisalign were divided into MC and non-midcourse correction groups according to whether they completed the first series of aligners. Differences between these groups were compared with independent sample t tests, chi-square tests, and Wilcoxon rank sum tests. A multivariate logistic regression analysis was performed to identify independent risk factors, including gender, age, extraction treatment, interproximal reduction, correction steps (steps in first series treatment), overbite, overjet, the curve of Spee, Angle classification, and crowding.

Results: The percentage of females (86.3%), Angle Class I malocclusion (62.4%), and nonextraction (56.1%) was relatively higher in all 314 patients. More than half of the patients (73.6%) completed the first series of aligners. Differences between the groups in the number of patients with extraction, correction steps, and the curve of Spee were significant (P <0.05). The proportions of MC were 41.3% and 14.8% in extraction and nonextraction patients, respectively. More initially planned correction steps were seen in the MC group (53.4 ± 15.6 steps). Extraction (adjusted odds ratio, 0.375; P = 0.001) and correction steps (adjusted odds ratio, 1.06; P <0.001) were independent risk factors for MC.

Conclusions: Extraction and the number of initially planned correction steps are independent risk factors for MC. In patients with complex dentofacial abnormalities, such as extraction, MC may be needed to achieve predicted changes.
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http://dx.doi.org/10.1016/j.ajodo.2021.12.018DOI Listing
June 2022

The multiple biotoxicity integrated study in grass carp (Ctenopharyngodon idella) caused by Ochratoxin A: Oxidative damage, apoptosis and immunosuppression.

J Hazard Mater 2022 08 2;436:129268. Epub 2022 Jun 2.

Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan 611130, China. Electronic address:

Ochratoxin A (OTA) is a common hazardous food contaminant that seriously endangers human and animal health. However, limited study is focused on aquatic animal. This research investigated the multiple biotoxicity of OTA on spleen (SP) and head kidney (HK) in grass carp and its related mechanism. Our data showed that, dietary supplemented with OTA above 1209 μg/kg caused histopathological damages by decreasing the number of lymphocytes and necrotizing renal parenchymal cells. Meanwhile, OTA caused oxidative damage and reduced the isoforms mRNAs transcripts of antioxidant enzymes (e.g., GPX1, GPX4, GSTO) partly due to suppressing NF-E2-related factor 2 (Nrf2). OTA triggered apoptosis through mitochondria and death receptor pathway potentially by p38 mitogen-activated protein kinase (p38MAPK) activation. Besides, OTA exacerbated inflammation by down-regulation of anti-inflammatory factor (e.g., IL-10, IL-4) and up-regulations of pro-inflammatory factors (e.g., TNF-α, IL-6), which could be ascribed to signaling meditation of Janus kinase / signal transducer and activator of transcription (JAK/STAT). Additionally, the safe upper limits of OTA were estimated to be 677.6 and 695.08 μg/kg based on the immune-related indexes (C3 contents in the SP and LZ activities in the HK, respectively). Our study has provided a wide insight for toxicological assessment of feed pollutant in aquatic animals.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129268DOI Listing
August 2022

Development of the Practice of Pharmaceutical Care for Cancer Pain Management in Outpatient Clinics Using the Delphi Method.

Front Pharmacol 2022 2;13:840560. Epub 2022 Jun 2.

Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, China.

There exists no broad agreement of experts on the practice of pharmaceutical care for cancer pain management in outpatient clinics. This study aimed to use the Delphi consensus process to provide expert recommendations on the practice of cancer pain management in outpatient clinics from the point of view of pharmaceutical care in clinical practice and future clinical trials. A comprehensive literature review was conducted to draft the initial practice. In this process, 30-40 senior experts from various provinces in China were invited to rank the items of practice during the two Delphi consultations. The definitions of consensus included a combination with an average score of ≥4, the percentage of experts rating the scores at >4 points, and the coefficient of variation of the scores. The expert panel comprised 18 pharmacists, 3 anesthesiologists, 6 oncologists, and 9 nurses. As a result of a comprehensive review, 33 items were initially formed. Among them, the consensus was reached for 27 items after the first Delphi round. The other six items and a total of five items for supplementation entered the second round, among which consensus was reached for eight items and three items were excluded. Expert consensus was achieved on 35 items after two rounds of consultation, which involved the collection of patient basic information, comprehensive pain assessment, breakthrough or neuropathic pain assessment, analgesic treatment evaluation, out-of-hospital follow-up, medical records, and evidence-based documents for reference. The final list of 35 items could be used to develop the practice of pharmaceutical care for cancer pain management in outpatient clinics in China. The practice may aid in the standardization of pharmaceutical care for pain, relieve pain to the greatest extent possible, and enhance the level of pain management in China.
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http://dx.doi.org/10.3389/fphar.2022.840560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201566PMC
June 2022

The Electrophysiological Characteristics and Possible Mechanism of Bipolar Pacing in Left Bundle Branch Pacing.

Heart Rhythm 2022 Jun 16. Epub 2022 Jun 16.

Department of Cardiology, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, Zhejiang, China. Electronic address:

Background: Left bundle branch pacing (LBBP) is a physiological pacing modality with a low and stable threshold. The electrophysiological characteristics and mechanisms of bipolar pacing remains unclear.

Objective: This study aimed to assess the electrophysiological characteristics of bipolar pacing of LBBP and to infer the mechanisms underlying each electrocardiogram (ECG) and electrogram (EGM) waveform morphology.

Methods: A total of 65 patients who strictly met the criteria for left bundle branch (LBB) capture were enrolled. The changes of morphology of ECG and EGM during the threshold test with different outputs of unipolar and bipolar pacing were recorded. The electrophysiological characteristics were then analyzed.

Results: Four distinct morphologies and three different types of transitions during bipolar pacing threshold testing were identified, we labeled the four types of morphologies as non-selective (NS)-bipolar-left bundle (LB), NS-cathodal-LB, selective (S)-cathodal-LB, left ventricular septal (LVS)-cathodal. Except LVS-cathodal, the other three types (NS-bipolar-LB, NS-cathodal-LB, S-cathodal-L) had short and constant V6 R-wave peak time (RWPT) (64.8 ± 7.7 ms vs. 65.7 ± 7.8 ms vs. 65.7 ± 7.3 ms). Among the four types, paced QRS (P-QRS) was the narrowest in NS-bipolar-LB rather than in NS-cathodal-LB (118.2 ± 14.2 ms vs. 133.8 ± 15.8 ms; p < 0.001). The NS-bipolar-LB had higher threshold than that of NS-cathodal-LB (2.5 ± 1.2 v vs. 0.8 ± 0.4 v; p < 0.001).

Conclusion: With a higher output on bipolar pacing, NS-bipolar-LB capture had the shortest V6 RWPT, V1 RWPT, and P-QRS. S-cathodal-LB capture had the longest V1 RWPT and P-QRS complex.
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http://dx.doi.org/10.1016/j.hrthm.2022.06.022DOI Listing
June 2022

LC-MS-based metabolomics reveals metabolic changes in short- and long-term administration of Compound Danshen Dripping Pills against acute myocardial infarction in rats.

Phytomedicine 2022 Jun 12;104:154269. Epub 2022 Jun 12.

State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address:

Background: Mild and systematically improving multiple metabolic disorders was a focused view for Compound Danshen Dripping Pills playing synergistic effects through multiple components and multiple targets. The difference in overall therapeutic effects and endogenous metabolic regulation between short- and long-term administration was still unclear.

Purpose: This study aimed to explore the difference in endogenous metabolic regulation between short- and long-term Compound Danshen Dripping Pills (CDDP) administration against acute myocardial infarction (AMI).

Methods: The model of AMI was induced by ligating the left anterior descending coronary artery. The cardiac protection effects of CDDP were investigated by echocardiography, 1- or 2-week were defined as short- and long-term based on desirable efficacy variability. The entire metabolic changes between short- and long-term administration of CDDP were profiled by UPLC-Q-TOF-MS. In addition, the metabolic regulatory network of CDDP administration against myocardial infarction rats was also compared with those of a typical chemical drug isosorbide 5-mononitrate (ISMN).

Results: After 1- or 2-week continuous oral administration, CDDP could significantly alleviate AMI-induced cardiac dysfunction. By using LC-MS-based metabolomics analyses, we systematically investigated the metabolic profiles of plasma and heart tissue samples at fixed exposure time-points (2 h, 24 h) from AMI rats with CDDP treatment. Most interestingly, global endogenous metabolic changes were observed in cardiac samples collected at different stages post consecutive CDDP administration, fluctuating at 2 and 24 h after 1 week but stabilizing after 2 weeks. The disrupted metabolic pathways such as glycerophospholipid, amino acids, fatty acids, and arachidonic acid metabolism were reconstructed after both short- and long-term CDDP treatment, while taurine and hypotaurine metabolism and purine metabolism contributed to the whole efficacy after long-term CDDP administration.

Conclusion: Long-term CDDP treatment plays prolonged and stable efficacy against AMI compared with short-term treatment by specifically regulating purine and taurine and hypotaurine metabolism and systematically redressing metabolic disorders.
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http://dx.doi.org/10.1016/j.phymed.2022.154269DOI Listing
June 2022

Related factors associated with fear of hypoglycemia in parents of children and adolescents with type 1 diabetes - A systematic review.

J Pediatr Nurs 2022 Jun 15;66:125-135. Epub 2022 Jun 15.

School of Nursing, Yangzhou University, Yangzhou, China; Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, China. Electronic address:

Problem: Fear of hypoglycemia is a significant concern for parents of children/ adolescents with type 1 diabetes. Although some studies have explained the parental fear of hypoglycemia, the related factors were yet to be determined. This systematic review aims to identify the related factors of fear of hypoglycemia in the parents of children and adolescents with type 1 diabetes and provide a theoretical basis for further intervention.

Eligibility Criteria: PubMed, MEDLINE, EMBASE, Scopus, CINAHL, EBSCO, Web of Science, and Cochrane Library were systematically searched from 2010 to 2021. Studies evaluating the fear of hypoglycemia of parents and its associated factors were included.

Sample: Twenty-three observational articles met the criteria.

Results: Significant associations were found between fear of hypoglycemia and specific factors, including motherhood, nocturnal hypoglycemia, and the number of blood glucose monitoring. Psychological factors, including anxiety, depression, pediatric parenting stress, mindfulness, self-efficacy, quality of life, and sleep disorders, were conclusive and associations with parental fear of hypoglycemia.

Conclusions: Understanding parental fear of hypoglycemia can help parents prevent potential problems in diabetes management, thus promoting children's growth. According to current evidence, effective targeted interventions based on modifiable relevant factors can be developed to reduce the fear of hypoglycemia in parents while maintaining optimal blood glucose control in children/ adolescents.

Implications: Health professionals should pay more attention to the mental health of parents, and parents should be involved in the care plan and have the opportunity to discuss their fear of hypoglycemia in the most appropriate way to manage type 1 diabetes.
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http://dx.doi.org/10.1016/j.pedn.2022.05.022DOI Listing
June 2022

Photothermolysis Mediated by Gold Nanorods Conjugated with Epidermal Growth Factor Receptor (EGFR) Monoclonal Antibody Induces Apoptosis via the Mitochondrial Apoptosis Pathway in Laryngeal Squamous Cell Cancer.

J Biomed Nanotechnol 2022 Mar;18(3):754-762

First Department of Head and Neck Surgery, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan, China.

Gold nanorods (AuNRs) have unique optical properties and biological affinity and can be used to treat tumors when conjugated with other protein molecules. Our previous studies have shown that EGFR monoclonal antibody (EGFRmAb)-modified AuNRs exert strong antitumor activity by inducing apoptosis. In this study, we tested the effects of EGFRmAb-modified AuNRs on laryngeal squamous cell cancer (LSCC) and . The results showed that EGFRmAb-modified AuNRs inhibited NP-69, BEAS-2B and Hep-2 cell growth and induced mitochondria-dependent apoptosis. The mitochondrial membrane potential was reduced, leading to the release of cytochrome C (Cyt C) and consequent activation of the intrinsic mitochondrial apoptosis pathway. Moreover, we observed that the occurrence of mitochondrial apoptosis is related to the destruction of the lysosome-mitochondria axis. To verify the effects , we also established a laryngeal tumor model in nude mice by subcutaneous transplantation. In model mice treated with EGFRmAb-modified AuNRs and irradiated with an NIR laser, tumor cell apoptosis and tumor growth were inhibited. These results suggest that EGFRmAb-modified AuNRs induced apoptosis through the intrinsic mitochondrial apoptotic pathway and are a potential candidate for cancer therapy.
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http://dx.doi.org/10.1166/jbn.2022.3272DOI Listing
March 2022

Adipocyte-derived kynurenine promotes obesity and insulin resistance by activating the AhR/STAT3/IL-6 signaling.

Nat Commun 2022 Jun 17;13(1):3489. Epub 2022 Jun 17.

Department of Respiratory and Critical Care Medicine, the Center for Biomedical Research, NHC Key Laboratory of Respiratory Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Aberrant amino acid metabolism is a common event in obesity. Particularly, subjects with obesity are characterized by the excessive plasma kynurenine (Kyn). However, the primary source of Kyn and its impact on metabolic syndrome are yet to be fully addressed. Herein, we show that the overexpressed indoleamine 2,3-dioxygenase 1 (IDO1) in adipocytes predominantly contributes to the excessive Kyn, indicating a central role of adipocytes in Kyn metabolism. Depletion of Ido1 in adipocytes abrogates Kyn accumulation, protecting mice against obesity. Mechanistically, Kyn impairs lipid homeostasis in adipocytes via activating the aryl hydrocarbon receptor (AhR)/Signal transducer and activator of transcription 3 /interleukin-6 signaling. Genetic ablation of AhR in adipocytes abolishes the effect of Kyn. Moreover, supplementation of vitamin B6 ameliorated Kyn accumulation, protecting mice from obesity. Collectively, our data support that adipocytes are the primary source of increased circulating Kyn, while elimination of accumulated Kyn could be a viable strategy against obesity.
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http://dx.doi.org/10.1038/s41467-022-31126-5DOI Listing
June 2022

Current status of carbon neutrality in Chinese rice fields (2002-2017) and strategies for its achievement.

Sci Total Environ 2022 Jun 14:156713. Epub 2022 Jun 14.

Macro Research Agricultural Institute, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

China has pledged to achieve carbon neutrality by 2060 to address global climate change, and achieving carbon neutrality in rice fields is a vital component of this commitment. However, the current status of carbon neutrality in rice fields in China is unclear, and there are few feasible strategies to achieve its successful implementation. Therefore, this study calculated the net carbon sequestration rate (NSOCR, i.e., carbon sequestration minus carbon emissions) of rice fields in China from 2002 to 2017 to clarify the carbon neutrality status of Chinese rice fields. Furthermore, the effects of field management measures, rice sown area, and rice yield on NSOCR were analyzed to identify suitable carbon neutralization pathways in Chinese rice fields. Our findings indicated that the annual carbon sequestration rate in rice fields was lower than the carbon emissions, resulting in continuous net emissions of 195.49 Tg CO-eq yr. The NSOCR of paddy fields increased first and then decreased with increases in rice sown area and yield. Meta-analysis indicated that management measures such as water conservation and biochar significantly increased NSOCR by ~5766.50 kg CO-eq ha yr and 22,296.62 kg CO-eq ha yr, respectively. Our findings suggests that proper control of rice sown area and the adoption of reasonable field management measures (water conservation and biochar) can promote carbon neutrality in Chinese rice fields.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156713DOI Listing
June 2022

Magnetoelectrically ignited nanozyme-eel for combating bacterial biofilms.

Chem Commun (Camb) 2022 Jun 17. Epub 2022 Jun 17.

Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P. R. China.

A magnetoelectrically ignited nanozyme-eel was developed, which could generate abundant surface charges upon the ignition of an alternating magnetic field, leading to a controllable electron transport burst between the nanozyme-eel and bacteria for the eradication of bacterial biofilms.
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http://dx.doi.org/10.1039/d2cc02603aDOI Listing
June 2022

10-Hydroxydec-2-Enoic Acid Reduces Hydroxyl Free Radical-Induced Damage to Vascular Smooth Muscle Cells by Rescuing Protein and Energy Metabolism.

Front Nutr 2022 26;9:873892. Epub 2022 May 26.

Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing, China.

10-Hydroxydec-2-enoic acid (10-HDA), an unsaturated hydroxyl fatty acid from the natural food royal jelly, can protect against cell and tissue damage, yet the underlying mechanisms are still unexplored. We hypothesized that the neutralization of the hydroxyl free radical (•OH), the most reactive oxygen species, is an important factor underlying the cytoprotective effect of 10-HDA. In this study, we found that the •OH scavenging rate by 10-HDA (2%, g/ml) was more than 20%, which was achieved through multiple-step oxidization of the -OH group and C=C bond of 10-HDA. Moreover, 10-HDA significantly enhanced the viability of vascular smooth muscle cells (VSMCs) damaged by •OH ( < 0.01), significantly attenuated •OH-derived malondialdehyde production that represents cellular lipid peroxidation ( < 0.05), and significantly increased the glutathione levels in •OH-stressed VSMCs ( < 0.05), indicating the role of 10-HDA in reducing •OH-induced cytotoxicity. Further proteomic analyses of VSMCs identified 195 proteins with decreased expression by •OH challenge that were upregulated by 10-HDA rescue and were primarily involved in protein synthesis (such as translation, protein transport, ribosome, and RNA binding) and energy metabolism (such as fatty acid degradation and glycolysis/gluconeogenesis). Taken together, these findings indicate that 10-HDA can effectively promote cell survival by antagonizing •OH-induced injury in VSMCs. To the best of our knowledge, our results provide the first concrete evidence that 10-HDA-scavenged •OH could be a potential pharmacological application for maintaining vascular health.
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http://dx.doi.org/10.3389/fnut.2022.873892DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196250PMC
May 2022

Identification of Biomarkers Associated With CD4 T-Cell Infiltration With Gene Coexpression Network in Dermatomyositis.

Front Immunol 2022 30;13:854848. Epub 2022 May 30.

Department of Pediatrics, The Second Xiangya Hospital of Central South University, Changsha, China.

Background: Dermatomyositis is an autoimmune disease characterized by damage to the skin and muscles. CD4 T cells are of crucial importance in the occurrence and development of dermatomyositis (DM). However, there are few bioinformatics studies on potential pathogenic genes and immune cell infiltration of DM. Therefore, this study intended to explore CD4 T-cell infiltration-associated key genes in DM and construct a new model to predict the level of CD4 T-cell infiltration in DM.

Methods: GSE46239, GSE142807, GSE1551, and GSE193276 datasets were downloaded. The WGCNA and CIBERSORT algorithms were performed to identify the most correlated gene module with CD4 T cells. Matascape was used for GO enrichment and KEGG pathway analysis of the key gene module. LASSO regression analysis was used to identify the key genes and construct the prediction model. The correlation between the key genes and CD4 T-cell infiltration was investigated. GSEA was performed to research the underlying signaling pathways of the key genes. The key gene-correlated transcription factors were identified through the RcisTarget and Gene-motif rankings databases. The miRcode and DIANA-LncBase databases were used to build the lncRNA-miRNA-mRNA network.

Results: In the brown module, 5 key genes (chromosome 1 open reading frame 106 (), component of oligomeric Golgi complex 8 (), envoplakin (), GTPases of immunity-associated protein family member 6 (), and interferon-alpha inducible protein 6 ()) highly associated with CD4 T-cell infiltration were identified. The prediction model was constructed and showed better predictive performance in the training set, and this satisfactory model performance was validated in another skin biopsy dataset and a muscle biopsy dataset. The expression levels of the key genes promoted the CD4 T-cell infiltration. GSEA results revealed that the key genes were remarkably enriched in many immunity-associated pathways, such as JAK/STAT signaling pathway. The cisbp_M2205, transcription factor-binding site, was enriched in , , and . Finally, 3,835 lncRNAs and 52 miRNAs significantly correlated with key genes were used to build a ceRNA network.

Conclusion: The , , , , and genes are associated with CD4 T-cell infiltration. The prediction model constructed based on the 5 key genes may better predict the level of CD4 T-cell infiltration in damaged muscle and lesional skin of DM. These key genes could be recognized as potential biomarkers and immunotherapeutic targets of DM.
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http://dx.doi.org/10.3389/fimmu.2022.854848DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196312PMC
June 2022

Fluorination Enables Simultaneous Improvements of a Dialkoxybenzene-Based Redoxmer for Nonaqueous Redox Flow Batteries.

ACS Appl Mater Interfaces 2022 Jun 16;14(25):28834-28841. Epub 2022 Jun 16.

Joint Center for Energy Storage Research, Argonne National Laboratory, 9700 South Cass Avenue, Lemont, Illinois 60439, United States.

Redoxmers or redox-active organic materials, are one critical component for nonaqueous redox flow batteries (RFBs), which hold high promise in enabling the time domain of the grid. While tuning redox potentials of redoxmers is a very effective way to enhance energy densities of NRFBs, those improvements often accompany accelerated kinetics of the charged species, undermining stability and cycling performance. Herein, a strategy for designing redoxmers with simultaneous improvements in redox potential and stability is proposed. Specifically, the redoxmer 1,4-di--butyl-2,5-bis(2,2,2-trifluoroethoxy)benzene (ANL-C46) is developed by incorporating fluorinated substitutions into the dialkoxybenzene-based platform. Compared to the non-fluorinated analogue, ANL-C46 demonstrates not only an increased (∼0.41 V) redox potential but also much enhanced stability (1.6 times) and cyclability (4 times) evidenced by electron paramagnetic resonance kinetic study, H-cell and flow cell cycling. In fact, the cycling performance of ANL-C46 is among the best of high potential (>1.0 V vs Ag/Ag) redoxmers ever reported. Density functional theory calculations suggest that while the introduced fluorine substitutions elevate the redox potentials, they also help to depress the decomposition reactions of the charged redoxmers, affording excellent stability. The findings represent an interesting strategy for simultaneously improving energy density and stability, which could further prompt the development of high-performance redoxmers.
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http://dx.doi.org/10.1021/acsami.2c04926DOI Listing
June 2022

Effect of the knee replacement surgery on activity level based on ActivPAL: a systematic review and meta-analysis study.

BMC Musculoskelet Disord 2022 Jun 15;23(1):576. Epub 2022 Jun 15.

Institute of Physiotherapy and Sport Science, Faculty of Health Sciences, University of Pecs, Pécs, Hungary.

Background: The knee replacement (KR) surgery aims to restore the activity level and reduce the risk of experiencing disabilities. The outcomes of this surgery are evaluated mainly with subjective tools or low validity objective tools. However, the effect of the surgery on activity level using high validity objective accelerometer is still in question.

Methods: A systematic review and meta-analysis were conducted to evaluate the benefit of KR surgery alone to enhance physical activity recommendations based on high validity accelerometer. Two independent reviewers evaluated five electronic databases (Cochrane-Central-Register-of-Controlled Trials, EMBASE, PubMed, Web of Science, and Scopus) to find relative studies between January 2000 and October 2021. The quality assessments and risk of bias assessments were examined.

Results: Three articles were included with 202 participants (86 males, 116 females), with an average age of 64 years and an average 32 kg/m body mass index. The results found that the number of steps was significantly improved up to 36.35 and 45.5% after 6-months and 1-year of the surgery, respectively. However, these changes did not meet the recommended activity level guideline and could be related to the patients' health status and their activity level before the surgery. No significant changes were seen in sedentary time, standing time, and upright time after 6-months and 1-year follow-ups. Heterogeneity among studies was low to moderate (0-63%).

Conclusion: Knee replacement surgery is an effective treatment for improving patients' quality of life with severe knee injuries. However, various factors impact the success of surgical and achieving maximum benefit of the surgery. One factor, sedentary time, can be reduced by implementing pre-and post-surgery exercise or physical activity recommendations. Further studies are needed to understand the benefit of surgery with or without rehabilitation assessed using high validity monitors.
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http://dx.doi.org/10.1186/s12891-022-05531-2DOI Listing
June 2022

Effects of mutual intercropping on cadmium accumulation of Nakamura et Odashima and its post-grafting generations.

Int J Phytoremediation 2022 Jun 14:1-9. Epub 2022 Jun 14.

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.

The cadmium (Cd) contaminated agricultural soil has become serious in recent years, but it will take long time for Cd-hyperaccumulator to remedy. To speed up the remediation of agricultural soil and achieve the safe agricultural production as soon as possible, the potential Cd-hyperaccumulator Nakamura et Odashima was intercropped with its post-grafting generations in Cd-contaminated soil. Intercropping increased the biomass, Cd contents and Cd extractions of and its post-grafting generations in the pot and field experiments. Both the whole plant or shoot biomass and the Cd extraction by whole plant or shoot in intercroppings had a linear regression relationship with that in monocultures. In the field experiment, intercropping increased the shoot Cd extraction of by 9.86%-40.06% compared with the monoculture. Intercropping increased the content of chlorophyll, activity of superoxide dismutase, activity of catalase, and soluble protein content of and its post-grafting generations but reduced their peroxidase activities in the pot experiment. Therefore, intercropping with its post-grafting generations can improve their phytoremediation ability, and the best choice is intercropped with its post-grafting generation of wild potato rootstock. Intercropping Nakamura et Odashima with its post-grafting generations could mutually promote the Cd accumulation in the two types of plant species, and improve their phytoremediation ability for remedying the Cd-contaminated soil.
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http://dx.doi.org/10.1080/15226514.2022.2085239DOI Listing
June 2022

Hepatitis B Virus X Protein Is Stabilized by the Deubiquitinating Enzyme VCPIP1 in a Ubiquitin-Independent Manner by Recruiting the 26S Proteasome Subunit PSMC3.

J Virol 2022 Jun 13:e0061122. Epub 2022 Jun 13.

Key Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.

Hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) is the sixth most common cancer worldwide, and the viral X protein (HBx) is an etiological factor in HCC development. HBx is a high-turnover protein, but knowledge of the role of deubiquitinating enzymes (DUBs) in maintaining HBx homeostasis is very limited. We used a 74-DUB library-based yeast two-hybrid assay and determined that a novel DUB, valosin-containing protein-interacting protein 1 (VCPIP1), interacted with HBx. VCPIP1 and its C-terminal amino acids 863 to 1221 upregulated the HBx protein expression, with or without HBV infection. Mechanistically, VCPIP1 stabilized HBx protein through a ubiquitin-independent pathway, which was validated by the HBx ubiquitination site mutant plasmid. Coimmunoprecipitation assays demonstrated the potency of VCPIP1 in recruiting 26S proteasome regulatory subunit 6A (PSMC3) and forming a ternary complex with HBx through mutual interaction. , purified His-tagged PSMC3 protein rescued HBx degradation induced by the 20S proteasome, and VCPIP1 synergized the mechanism. Functionally, HBx specifically binding to VCPIP1 significantly enhanced the transcriptional transactivation of HBx by activating NF-κB, AP-1, and SP-1 and inhibited hepatoma cell clonogenicity in Huh7 and HepG2 cells. Moreover, we further demonstrated that overexpression of VCPIP1 significantly affected the HBV covalently closed circular DNA (cccDNA) transcription in HBV-infected HepG2-NTCP cells. Altogether, our results indicate a novel mechanism by which VCPIP1 recruits PSMC3 to bind with HBx, stabilizing it in a ubiquitin-independent manner, which might be critical for developing DUB inhibitors in the future. HBx is a multifunctional viral oncoprotein that plays an essential role in the viral life cycle and hepatocarcinogenesis. HBx degradation occurs through the ubiquitin-proteasome system (UPS). However, whether novel compartments of the DUBs in the UPS also act in regulating HBx stability is not fully understood. Here, for the first time, we defined VCPIP1 as a novel DUB for preventing HBx degradation by the 20S proteasome in a ubiquitin-independent manner. PSMC3, encoding the 26S proteasome regulatory subunit, directly stabilized HBx through physical binding instead of a common approach in protein degradation, serving as the key downstream effector of VCPIP1 on HBx. Therefore, the ternary binding pattern between VCPIP1, HBx, and PSMC3 is initiated for the first time, which eventually promotes HBx stability and its functions. Our findings provide novel insights into host-virus cross talk by targeting DUBs in the UPS.
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http://dx.doi.org/10.1128/jvi.00611-22DOI Listing
June 2022

Heterometallic nanomaterials: activity modulation, sensing, imaging and therapy.

Chem Sci 2022 May 12;13(19):5505-5530. Epub 2022 Apr 12.

Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Life Sciences, College of Geography and Environmental Sciences, Zhejiang Normal University Jinhua 321004 China

Heterometallic nanomaterials (HMNMs) display superior physicochemical properties and stability to monometallic counterparts, accompanied by wider applications in the fields of catalysis, sensing, imaging, and therapy due to synergistic effects between multi-metals in HMNMs. So far, most reviews have mainly concentrated on introduction of their preparation approaches, morphology control and applications in catalysis, assay of heavy metal ions, and antimicrobial activity. Therefore, it is very important to summarize the latest investigations of activity modulation of HMNMs and their recent applications in sensing, imaging and therapy. Taking the above into consideration, we briefly underline appealing chemical/physical properties of HMNMs chiefly tailored through the sizes, shapes, compositions, structures and surface modification. Then, we particularly emphasize their widespread applications in sensing of targets ( metal ions, small molecules, proteins, nucleic acids, and cancer cells), imaging (frequently involving photoluminescence, fluorescence, Raman, electrochemiluminescence, magnetic resonance, X-ray computed tomography, photoacoustic imaging, ), and therapy ( radiotherapy, chemotherapy, photothermal therapy, photodynamic therapy, and chemodynamic therapy). Finally, we present an outlook on their forthcoming directions. This timely review would be of great significance for attracting researchers from different disciplines in developing novel HMNMs.
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http://dx.doi.org/10.1039/d2sc00460gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9116289PMC
May 2022

A Novel Key Features Screening Method Based on Extreme Learning Machine for Alzheimer's Disease Study.

Front Aging Neurosci 2022 25;14:888575. Epub 2022 May 25.

College of Information Engineering Shanghai Maritime University, Shanghai, China.

The Extreme Learning Machine (ELM) is a simple and efficient Single Hidden Layer Feedforward Neural Network(SLFN) algorithm. In recent years, it has been gradually used in the study of Alzheimer's disease (AD). When using ELM to diagnose AD based on high-dimensional features, there are often some features that have no positive impact on the diagnosis, while others have a significant impact on the diagnosis. In this paper, a novel Key Features Screening Method based on Extreme Learning Machine (KFS-ELM) is proposed. It can screen for key features that are relevant to the classification (diagnosis). It can also assign weights to key features based on their importance. We designed an experiment to screen for key features of AD. A total of 920 key functional connections screened from 4005 functional connections. Their weights were also obtained. The results of the experiment showed that: (1) Using all (4,005) features to diagnose AD, the accuracy is 95.33%. Using 920 key features to diagnose AD, the accuracy is 99.20%. The 3,085 (4,005 - 920) features that were screened out had a negative effect on the diagnosis of AD. This indicates the KFS-ELM is effective in screening key features. (2) The higher the weight of the key features and the smaller their number, the greater their impact on AD diagnosis. This indicates that the KFS-ELM is rational in assigning weights to the key features for their importance. Therefore, KFS-ELM can be used as a tool for studying features and also for improving classification accuracy.
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http://dx.doi.org/10.3389/fnagi.2022.888575DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177228PMC
May 2022

Activin B-activated Cdc42 signaling plays a key role in regulating adipose-derived mesenchymal stem cells-mediated skin wound healing.

Stem Cell Res Ther 2022 Jun 11;13(1):248. Epub 2022 Jun 11.

Department of Histology and Embryology, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Key Laboratory of Construction and Detection in Tissue Engineering of Guangdong Province, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, People's Republic of China.

Background: In our previous study, activin B in combination with ADSCs enhances skin wound healing. However, the underlying molecular mechanisms are not well studied. Cdc42 is recognized to play a critical role in the regulation of stem cells.

Methods: Pull-down assay was performed to investigate the activity of Cdc42. The dominant-negative mutant of Cdc42 (Cdc42N17) was used to explore the role of Cdc42 in activin B-induced ADSCs migration, proliferation, and secretion in vitro. Cdc42N17-transfected ADSCs were injected into a full-thickness excisional wound model to explore their efficiency in wound healing in vivo. The wound healing efficacy was evaluated by the wound closure rates and histological examination. The neovascularization and wound contraction were detected by immunohistochemistry staining of CD31 and α-SMA. Finally, the underlying mechanisms were explored by RNA sequencing.

Results: Cdc42N17 inhibited ADSCs migration, proliferation, and secretion induced by activin B. Furthermore, Cdc42N17-transfected ADSCs inhibited the wound closure rate and suppressed the expression of CD31 and α-SMA induced by activin B in vivo. The RNA sequencing showed that the differentially expressed genes in Cdc42N17-transfected ADSCs versus ADSCs were associated with cell migration, proliferation, and adhesion. Further study revealed that the Cdc42-Erk-Srf pathway was required for activin B-induced proliferation in ADSCs.

Conclusions: Our study indicates that Cdc42 plays a crucial role in ADSCs-mediated skin wound healing induced by activin B.
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http://dx.doi.org/10.1186/s13287-022-02918-9DOI Listing
June 2022

Sediment organic matter properties facilitate understanding nitrogen transformation potentials in East African lakes.

Sci Total Environ 2022 Jun 9;841:156607. Epub 2022 Jun 9.

State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China. Electronic address:

East African lakes include the most productive and alkaline lake group in the world. Yet, they generally receive fewer nutrient inputs than the densely populated subtropical and temperate lakes in the northern hemisphere. In these lakes with insufficient supplies of inorganic nitrogen, the mineralization of benthic organic matter can play an important role in driving the nutrient cycle and nitrogen loss. Using a suite of stable N isotope dilution and tracer techniques, we examined five main processes of the sediment nitrogen cycle in 16 lakes and reservoirs of Tanzania and Kenya, East Africa: gross nitrogen mineralization, ammonium immobilization, dissimilatory nitrate reduction to ammonium (DNRA), and the dinitrogen (N) production via denitrification and anaerobic ammonium oxidation (anammox). Gross nitrogen mineralization and ammonium immobilization showed the maximum values of 9.84 and 12.39 μmol N kg h, respectively. Potential DNRA rates ranged from 0.22 to 8.15 μmol N kg h and accounted for 10 %-74 % (average 25 %) of the total dissimilatory nitrate reduction. Potential nitrate reduction rates in most lakes were dominated by denitrification with a contribution of 26 %-85 % and a mean of 65 %. We further found that the sediment nitrogen transformations were driven mainly by benthic organic matter properties and water column phosphate concentrations, reflecting microbial metabolic responses to the changing carbon and nutrients availability. For instance, autochthonous production of protein-like organic matter attributed to active sediment nitrogen mineralization, DNRA, and denitrification. In contrast, the high degree of humification caused by the inputs of terrestrial humic-like substances slowed down the sediment nitrogen transformations. The contribution of DNRA to total dissimilatory nitrate reduction was significantly positively correlated to sediment C: N ratios. These results indicate that predictions of sediment N supply and loss in East African lakes can be improved by incorporating sediment organic matter properties.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156607DOI Listing
June 2022

A highly conserved core bacterial microbiota with nitrogen-fixation capacity inhabits the xylem sap in maize plants.

Nat Commun 2022 Jun 11;13(1):3361. Epub 2022 Jun 11.

Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, PR China.

Microbiomes are important for crop performance. However, a deeper knowledge of crop-associated microbial communities is needed to harness beneficial host-microbe interactions. Here, by assessing the assembly and functions of maize microbiomes across soil types, climate zones, and genotypes, we found that the stem xylem selectively recruits highly conserved microbes dominated by Gammaproteobacteria. We showed that the proportion of bacterial taxa carrying the nitrogenase gene (nifH) was larger in stem xylem than in other organs such as root and leaf endosphere. Of the 25 core bacterial taxa identified in xylem sap, several isolated strains were confirmed to be active nitrogen-fixers or to assist with biological nitrogen fixation. On this basis, we established synthetic communities (SynComs) consisting of two core diazotrophs and two helpers. GFP-tagged strains and N isotopic dilution method demonstrated that these SynComs do thrive and contribute, through biological nitrogen fixation, 11.8% of the total N accumulated in maize stems. These core taxa in xylem sap represent an untapped resource that can be exploited to increase crop productivity.
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http://dx.doi.org/10.1038/s41467-022-31113-wDOI Listing
June 2022

Development and Influencing Factors of International Trade in Digitally Deliverable Services.

Front Psychol 2022 24;13:908420. Epub 2022 May 24.

School of International Economics and Management, Beijing Technology and Business University, Beijing, China.

Introduction: International trade in digitally deliverable services has been developing rapidly in recent years, especially during pandemics. However, the growth rate and scale differ among regions and countries. To promote the growth and bridge the divide of global trade in digitally deliverable services, we provide feasible policy suggestions in this paper.

Methods: Based on panel data of 33 countries' digitally deliverable service trade from 2005 to 2020, this study analyzes the development trends and influencing factors of international trade in digital-global service trade.

Results: The development of digitally deliverable services in developed countries is far ahead, while in emerging economies such as China and India, it is growing at a high speed. Digital infrastructure; human capital; and science, technology, and innovation capacity have a significant impact on the digitally deliverable trade of countries with the level of STI playing the most important influence. Further, heterogeneity analysis shows that human capital and STI capacity are more sensitive to the digitally deliverable trade of middle-income countries than to the impact of high-income countries, and the contribution of population in middle-income countries is more pronounced.

Conclusion: As the proportion of digital trade in global trade continues to increase, the role of digital service trade in countries' sustainable development is becoming more prominent. To cope with such opportunities and challenges, countries, especially developing countries, should actively increase digital infrastructure construction, improve talent training, vigorously develop digital technology, and enhance their innovation capacity.
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http://dx.doi.org/10.3389/fpsyg.2022.908420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171366PMC
May 2022
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