Publications by authors named "Lu Zhai"

55 Publications

Underlying Mechanism and Active Ingredients of Tianma Gouteng Acting on Cerebral Infarction as Determined Network Pharmacology Analysis Combined With Experimental Validation.

Front Pharmacol 2021 16;12:760503. Epub 2021 Nov 16.

Northeast Asian Research Institute of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun, China.

Cerebral infarction (CI), a common cerebrovascular disease worldwide, is caused by unknown factors common to many diseases, including hypokalemia, respiratory diseases, and lower extremity venous thrombosis. Tianma Gouteng (TMGT), a traditional Chinese Medicine (TCM) prescription, has been used for the clinical treatment of CI. In this study, high-performance liquid chromatography (HPLC) fingerprint analysis was used to detect and identify major chemical constituents of TMGT. TCMSP and BATMAN-TCM databases were used to screen for active TMGT constituent compounds, while the GeneCards database was used to screen for protein targets associated with CI. Next, GO and KEGG enrichment analysis of these core nodes were performed to determine the identities of key associated biological processes and signal pathways. Meanwhile, a total of six possible gene targets of TMGT, including NFKBIA, PPARG, IL6, IL1B, CXCL8, and HIF1A, were selected for further study using two cellular models of CI. For one model, PC12 cells were treated under oxygen and glucose deprivation (OGD) conditions to generate an OGD cellular model of CI, while for the other model, BV2 cells were stimulated with lipopolysaccharide (LPS) to generate a cellular model of CI-associated inflammation. Ultimately TMGT treatment increased PPARγ expression and downregulated the expression of p-P65, p-IκBα, and HIF-1α in both OGD-induced and LPS-induced cell models of CI. In addition, molecular docking analysis showed that one TMGT chemical constituent, quercetin, may be a bioactive TMGT compound with activity that may be associated with the alleviation of neuronal damage and neuroinflammation triggered by CI. Moreover, additional data obtained in this work revealed that TMGT could inhibit neuroinflammation and protect brain cells from OGD-induced and LPS-induced damage by altering HIF-1α/PPARγ/NF-κB pathway functions. Thus, targeting this pathway through TMGT administration to CI patients may be a strategy for alleviating nerve injury and neuroinflammation triggered by CI.
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http://dx.doi.org/10.3389/fphar.2021.760503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8635202PMC
November 2021

Directed movement changes coexistence outcomes in heterogeneous environments.

Ecol Lett 2021 Nov 24. Epub 2021 Nov 24.

Department of Environmental Science and Policy, University of California, Davis, Davis, California, USA.

Understanding mechanisms of coexistence is a central topic in ecology. Mathematical analysis of models of competition between two identical species moving at different rates of symmetric diffusion in heterogeneous environments show that the slower mover excludes the faster one. The models have not been tested empirically and lack inclusions of a component of directed movement toward favourable areas. To address these gaps, we extended previous theory by explicitly including exploitable resource dynamics and directed movement. We tested the mathematical results experimentally using laboratory populations of the nematode worm, Caenorhabditis elegans. Our results not only support the previous theory that the species diffusing at a slower rate prevails in heterogeneous environments but also reveal that moderate levels of a directed movement component on top of the diffusive movement allow species to coexist. Our results broaden the theory of species coexistence in heterogeneous space and provide empirical confirmation of the mathematical predictions.
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http://dx.doi.org/10.1111/ele.13925DOI Listing
November 2021

Incidence and effects of deep vein thrombosis on the outcome of patients with coronavirus disease 2019 infection.

J Vasc Surg Venous Lymphat Disord 2021 Nov 11. Epub 2021 Nov 11.

Department of Surgery, Jacobi Medical Center, Albert Einstein College of Medicine, Bronx, NY.

Background: Deep vein thrombosis (DVT) has been reported to occur at different rates in patients with coronavirus disease 2019 (COVID-19). Limited data exist regarding comparisons with non-COVID-19 patients with similar characteristics. Our objective was to compare the rates of DVT in patients with and without COVID-19 and to determine the effect of DVT on the outcomes.

Methods: We performed a retrospective, observational cohort study at a single-institution, level 1 trauma center comparing patients with and without COVID-19. The 573 non-COVID-19 patients (age, 61 ± 17 years; 44.9% male) had been treated from March 20, 2019 to June 30, 2019, and the 213 COVID-19 patients (age, 61 ± 16 years; 61.0% male) had been treated during the same interval in 2020. Standard prophylactic anticoagulation therapy consisted of 5000 U of heparin three times daily for the medical patients without COVID-19 who were not in the intensive care unit (ICU). The ICU, surgical, and trauma patients without COVID-19 had received 40 mg of enoxaparin daily (not adjusted to weight). The patients with COVID-19 had also received enoxaparin 40 mg daily (also not adjusted to weight), regardless of whether treated in the ICU. The two primary outcomes were the rate of deep vein thrombosis (DVT) in the COVID-19 group vs that in the historic control and the effect of DVT on mortality. The subgroup analyses included patients with adult respiratory distress syndrome (ARDS), pulmonary embolism (PE), and intensive care unit patients (ICU).

Results: The rate of DVT and PE for the non-COVID-19 patients was 12.4% (71 of 573) and 3.3% (19 of 573) compared with 33.8% (72 of 213) and 7.0% (15 of 213) for the COVID-19 patients, respectively. Unprovoked PE had developed in 10 of 15 COVID-19 patients (66.7%) compared with 8 of 497 non-COVID-19 patients (1.6%). The 60 COVID-19 patients with ARDS had had an incidence of DVT of 46.7% (n = 28). In contrast, the incidence of DVT for the 153 non-COVID-19 patients with ARDS was 28.8% (n = 44; P = .01). The COVID-19 patients requiring the ICU had had an increased rate of DVT (39 of 90; 43.3%) compared with the non-COVID-19 patients (33 of 123; 33.3%; P = .01). The risk factors for mortality included age, DVT, multiple organ failure syndrome, and prolonged ventilatory support with the following odd ratios: 1.030 (95% confidence interval [CI], 1.002-1.058), 2.847 (95% CI, 1.356-5.5979), 4.438 (95% CI, 1.973-9.985), and 5.321 (95% CI, 1.973-14.082), respectively.

Conclusions: The incidence of DVT for COVID-19 patients receiving standard-dose prophylactic anticoagulation that was not weight adjusted was high, especially for ICU patients. DVT is one of the factors contributing to increased mortality. These results suggest a reevaluation is necessary of the present standard-dose thromboprophylaxis for patients with COVID-19.
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http://dx.doi.org/10.1016/j.jvsv.2021.10.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8582229PMC
November 2021

Contrasting plant responses to multivariate environmental variations among species with divergent elevation shifts.

Ecol Appl 2021 Oct 22:e02488. Epub 2021 Oct 22.

Department of Natural Resource Ecology and Management, Oklahoma State University, Stillwater, Oklahoma, 74078, USA.

The general predictions of climate impacts on species shifts (e.g., upward shift) cannot directly inform local species conservation, because local-scale studies find divergent patterns instead of a general one. For example, our previous study found three shift patterns with elevation (strong down-, moderate down-, and up-slope shifts) in temperate mountain forests. The divergent shifts are hypothesized to arise from both multivariate environmental variations with elevation and corresponding species-specific responses. To test this hypothesis, we sampled soils and leaves to measure elevation variations in soil conditions and determined plant responses using discriminations against heavier isotopes, carbon ( C) and nitrogen ( N). Functional traits of the species studied were also extracted from a public trait dataset. We found that: (1) With low soil water contents at low elevations, only the leaves of up-shifters had lower C discriminations at low vs. high elevations; (2) With low soil P contents at high elevations, only the leaves of moderate down-shifters had higher N discriminations at high vs. low elevations; (3) The leaves of strong down-shifters did not show significant elevation patterns of the discriminations; (4) The contrasting responses among the three types of shifters agree with their functional dissimilarity, suggested by their separate locations in a multitrait space. Taken together, the divergent shifts are associated with the elevation variations in environmental conditions and contrasting plant responses. The contrasting responses could result from the functional dissimilarity among species. Therefore, a detailed understanding of both local environmental variations and species-specific responses can facilitate accurate predictions of species shifts to inform local species conservation.
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http://dx.doi.org/10.1002/eap.2488DOI Listing
October 2021

A Novel Biochemical Study of Anti-Dermal Fibroblast Replicative Senescence Potential of Oligosaccharides.

Front Pharmacol 2021 30;12:690538. Epub 2021 Jun 30.

Research Center of Traditional Chinese Medicine, the Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, China.

Dermal fibroblast replicative senescence that often occurs in aging skin is characterized by loss of cell proliferative capacity, cell cycle arrest, decreased cell elongation, and decreased synthesis of dermal extracellular matrix (ECM) components. Although is known for its effectiveness in alleviating many age-related degenerative diseases, few studies have evaluated components for efficacy or mechanisms of action in delaying cell replicative senescence. In this study, oligosaccharides (PNO) were isolated using a stepwise purification procedure involving water extraction and alcohol precipitation followed by DEAE Sepharose Fast Flow column chromatography, preparative high performance liquid chromatography, and size-exclusion chromatography. Monosaccharides detected in PNO constituents included mannose, galactose, and sorbitose in relative molar proportions of 14.2:12.3:1, respectively, aligning with PNO absorption spectrum results resembling typical known spectra for sugars. , PNO treatment of replicative senescent NIH-3T3 fibroblasts significantly promoted cell vitality, inhibited SA-β-galactosidase (SA-β-Gal) activity, and reduced p16 and p21 protein-level expression. Moreover, PNO treatment of senescent fibroblasts led to a lower proportion of G1 phase cells and higher proportion of S phase cells, while also inducing aging NIH-3T3 cells to migrate and synthesize collagen-I (CoL-I). Mechanistically, PNO treatment up-regulated expression of proliferating cell nuclear antigen (PCNA), cyclin E, cyclin D1, and cyclin-dependent kinase 4 (CDK4) proteins and promoted phosphorylation of MEK, p38, and ERK1/2 to trigger cell cycle progression. Additionally, PNO treatment also up-regulated protein-level expression of TGF-β1 and levels of p-Smad2/3, p-FAK, and p-Pax to trigger CoL-I synthesis and cell migration. Taken together, these findings demonstrate that oligosaccharides purified from could reverse fibroblast replicative senescence by promoting fibroblast cell proliferation, migration, and CoL-I production.
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http://dx.doi.org/10.3389/fphar.2021.690538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277921PMC
June 2021

The optimal controlling strategy on a dispersing population in a two-patch system: Experimental and theoretical perspectives.

J Theor Biol 2021 11 15;528:110835. Epub 2021 Jul 15.

Department of Biology, University of Miami, United States. Electronic address:

Invasive species, disease vectors, and pathogens are significant threats to biodiversity, ecosystem function and services, and human health. Understanding the optimal management strategy, which maximizes the effectiveness is crucial. Despite an abundance of theoretical work has conducted on projecting the optimal allocation strategy, almost no empirical work has been performed to validate the theory. We first used a consumer-resource model to simulate a series of allocation fractions of controlling treatment to determine the optimal controlling strategy. Further, we conducted rigorous laboratory experiments using spatially diffusing laboratory populations of yeast to verify our mathematical results. We found consistent results that: (1) When population growth is limited by the local resource, the controlling priority should be given to the areas with higher concentration of resource; (2) When population growth is not limited by the resource concentration, the best strategy is to allocate equal amount of controlling efforts among the regions; (3) With restricted budget, it is more efficient to prioritize the controlling effects to the areas with high population abundance, otherwise, it is better to control equally among the regions. The new theory, which was tested by laboratory experiments, will reveal new opportunities for future field interventions, thereby informing subsequent biological decision-making.
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http://dx.doi.org/10.1016/j.jtbi.2021.110835DOI Listing
November 2021

Ginsenoside extract from ginseng extends lifespan and health span in .

Food Funct 2021 Aug;12(15):6793-6808

Research Center of Traditional Chinese Medicine, the First Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, China.

Nutrition intervention has become a potential strategy to improve healthspan and prolong lifespan. Ginseng has been used for thousands of years and developed as a functional food to provide various protective effects to humans. An extract of total ginsenosides (TGS), a mixture of the main active ginsenosides from ginseng, has wide biological activities and health benefits for age-related diseases, including antioxidation and improvements in mitochondrial function. However, the molecular mechanism of TGS for prolonging lifespan and improving fitness and how exactly this is achieved under normal and stress conditions remain largely unclear. In this study, wild-type and mutant C. elegans strains are used to investigate the role and molecular mechanism of TGS-mediated longevity, health benefits, and stress resistance. The results showed that treatment with TGS at 0.2 mg mL-1 from the stage of day four to death significantly extended the lifespan of worms by 14.02% without effects on bacterial metabolism and food intake. Furthermore, TGS treatment obviously improved age-associated mobility, muscle fiber organization, lipofuscin accumulation, and enhanced resistance under oxidative stress. Importantly, these effects of TGS were achieved by activating the signaling pathways of anti-oxidant regulation and longevity, including the NRF2/SKN-1, SIRT1/SIR 2.1, and FOXO/DAF-16 signaling pathways. Finally, it was found that Rg1, Re, and Rb1 were the major effective components of anti-oxidative activity and longevity. Collectively, the protective effect of ginsenoside extract in healthy aging and stress responses provides new insights for the development and practical application of ginseng functional products.
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http://dx.doi.org/10.1039/d1fo00576fDOI Listing
August 2021

Scaling up experimental stress responses of grass invasion to predictions of continental-level range suitability.

Ecology 2021 08 13;102(8):e03417. Epub 2021 Jul 13.

School of Forest Resources and Conservation, Fort Lauderdale Research and Education Center, University of Florida, 3205 College Avenue, Davie, Florida, 33314, USA.

Understanding how the biological invasion is driven by environmental factors will improve model prediction and advance early detection, especially in the context of accelerating anthropogenic ecological changes. Although a large body of studies has examined how favorable environments promote biological invasions, a more comprehensive and mechanistic understanding of invasive species response to unfavorable/stressful conditions is still developing. Grass invasion has been problematic across the globe; in particular, C grass invaders, with high drought tolerance, adaptations to high temperatures, and high water use efficiency, could become more severe. Here, we conducted a rigorous microcosm experiment, with one of the most damaging invasive C grass, cogongrass (Imperata cylindrica), to explore how cogongrass responds to soil water and nutrient stress. We further integrated the results of the microcosm study with a species distribution model to (1) corroborate greenhouse results with field observations and (2) validate the robustness of our findings at subcontinental scales. Both the microcosm experiments and species distribution model agreed that soil water stress had a stronger impact on cogongrass than the nutrient one. New vegetative growth of cogongrass continued to be inhibited by the prior water stress. The significant water effect on cogongrass total biomass was supported by the finding that both allometric and biochemical traits of cogongrass did not show significant responses to the changes in water treatment. Different to the conventional wisdom that nutrient enrichment plays a bigger role in facilitating biological invasions, this study highlighted the possibility that water conditions may have a more substantial effect on some aggressive invaders. Therefore, an important implication of this study on biological conservation is that field managers might take advantage of the negative effect of global drought on some invasive species to increase the efficiency of their controlling efforts because invasive species may become more vulnerable under drought effect.
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http://dx.doi.org/10.1002/ecy.3417DOI Listing
August 2021

p16 loss facilitate hydroquinone-induced malignant transformation of TK6 cells through promoting cell proliferation and accelerating the cell cycle progression.

Environ Toxicol 2021 Aug 1;36(8):1591-1599. Epub 2021 May 1.

Department of Environmental and Occupational Health, Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China.

The p16 is a multifunction gene that includes regulation of the cell cycle, apoptosis, senescence and tumor development. However, the effects of p16 in hydroquinone-induced malignant transformation of TK6 cells remain unclear. The present study aimed to explore whether p16 loss facilitate malignant transformation in TK6 cells. The results demonstrated that p16/Rb signal pathway was suppressed in hydroquinone-induced malignant transformation of TK6 cells. We further confirmed that p16 loss stimulated cell proliferation, and accelerated cell cycle progression in vitro and in vivo. The immunoblotting analysis indicated that p16 regulated cell cycle progression via Rb and p53. Therefore, we conclude that p16 is involved in HQ-induced malignant transformation associated with suppressing Rb and p53 which resulting in accelerating the cell cycle progression.
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http://dx.doi.org/10.1002/tox.23155DOI Listing
August 2021

PARP-1 via regulation of p53 and p16, is involved in the hydroquinone-induced malignant transformation of TK6 cells by decelerating the cell cycle.

Toxicol In Vitro 2021 Aug 24;74:105153. Epub 2021 Mar 24.

Department of Environmental and Occupational Health, Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China. Electronic address:

Poly(ADP-ribose)polymerase-1 (PARP-1) plays a crucial role in DNA damage repair and could be viewed as both a tumor promoter and tumor-suppressor gene. However, the effects of PARP-1 in hydroquinone-induced malignant transformation of TK6 cells remain to be further elucidated. The present research evaluated the potential mechanism of PARP-1 in hydroquinone-induced malignant transformation of TK6 cells. The results indicated that high PARP-1 inhibited TK6 cells malignant transformation after chronic exposure to HQ. We further confirmed that PARP-1 overexpression blocked cell proliferation, and decelerated cell cycle progression in vitro and in vivo. The immunoblotting analysis indicated that PARP-1 regulated cell cycle progression via p16/Rb and p53. Therefore, we conclude that PARP-1 is involved in HQ-induced malignant transformation associated with increasing p16/Rb and p53 which resulting in decelerating the cell cycle progression.
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http://dx.doi.org/10.1016/j.tiv.2021.105153DOI Listing
August 2021

Quality of Life in Patients with β-thalassemia Major: Short-term and Long-term Effects After Haematopoietic Stem Cell Transplantation.

Curr Stem Cell Res Ther 2021 ;16(8):924-930

Department of Stem Cell Transplantation, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Background: Allogeneic haematopoietic stem cell transplantation (ALLO-HSCT) is a potentially curative approach to treat β-thalassemia major (β-TM).

Objective And Methods: To assess the quality of life (QOL) of patients with β-TM after ALLO-HSCT, we searched PubMed, Embase, Web of Science, and MEDLINE for articles on the quality of life (QOL) of patients with β-TM from 1 Feb 2020 to 31 Mar 2020.

Results: Our review revealed that the QOL of patients with β-TM after ALLO-HSCT from a sibling donor is higher than that of patients that received blood infusion and iron-chelating therapy. Survivors of ALLO-HSCT have a QOL as good as that of a healthy population and the ability to return to normal life. However, studies thus far are limited to investigations with a few patients with β-TM who received ALLO-HSCT of the bone marrow (BM) from a sibling donor or related donor. Graft vs. host disease, patient age, gender, sexual desire, health condition, psychological state, financial and employment stress, and social support contributed to a worse QOL after ALLO-HSCT. Medicine usage, physical therapy, and psychological intervention may help improve the decline in QOL related to ALLO-HSCT in patients with β-TM.

Conclusion: Doctors and nurses must focus on implementing medicine usage, physical therapy, and psychological intervention to improve the decline in QOL related to ALLO-HSCT.
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http://dx.doi.org/10.2174/1574888X16666210203105340DOI Listing
October 2021

Functions of mineral-solubilizing microbes and a water retaining agent for the remediation of abandoned mine sites.

Sci Total Environ 2021 Mar 27;761:143215. Epub 2020 Oct 27.

Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Province Key Laboratory of Soil and Water Conservation and Ecological Restoration, Nanjing Forestry University, 159 Longpan Road, Nanjing, Jiangsu 210037, China. Electronic address:

There has been a rapid increase in abandoned mines across China, Consequently, external-soil spray seeding technologies have emerged as a common method for their remediation. However, slope soils are typically unstable and easily collapsed and the nutrients absorbed by plants are insufficient, which complicate ecological restoration. For this study, we added mineral-solubilizing microbes and a water retaining agent to an external-soil spray seeding substrate in Lespedeza bicolor pots. We investigated the soil nutrients, soil enzyme activities, root growth parameters, root tensile properties, and root-reinforced soil shear strengths. The results revealed that the addition of microbes enhanced soil nutrients, soil enzyme activities, and the content of lignin and hemicellulose, which promoted root growth. Further, the addition of a water retaining agent promoted Lespedeza bicolor root growth but decreased the root tensile strength and force. Shear stress under the microbe treatment was more robust than without it. Finally, root growth was correlated with soil nutrients and enzyme activities, whereas the root tensile force and strength were correlated with lignin and cellulose. Our results suggested that the addition of mineral-solubilizing microbes had the capacity to enhance the quality of soils to facilitate the growth of plants. These results provide a new and viable strategy for the ecological restoration of abandon mine sites.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143215DOI Listing
March 2021

Shen-Hong-Tong-Luo Formula Attenuates Macrophage Inflammation and Lipid Accumulation through the Activation of the PPAR-/LXR-/ABCA1 Pathway.

Oxid Med Cell Longev 2020 1;2020:3426925. Epub 2020 Oct 1.

Department of Cardiology, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China.

Atherosclerosis (AS) is the killer of human health and longevity, which is majorly caused by oxidized lipoproteins that attack macrophages in the endarterium. The Shen-Hong-Tong-Luo (SHTL) formula has shown great clinical efficacy and vascular protective effect for over 30 years in China, to attenuate AS progression. However, its pharmacological mechanism needs more investigation. In this study, we first investigated the chemical composition of SHTL by fingerprint analysis using high-performance liquid chromatography. In primary mouse peritoneal macrophages induced by lipopolysaccharide (LPS), we found that SHTL pretreatment suppressed reactive oxygen species accumulation and reversed the increases of the inflammatory factors, TNF- and IL-6. Moreover, lipid accumulation induced by oxidized low-density lipoprotein (Ox-LDL) in macrophages was inhibited by SHTL. Additionally, network pharmacology was used to predict the potential targets of SHTL as the PPAR-/LXR-/ABCA1 signaling pathway, which was validated in macrophages and ApoE mice by histopathological staining, qPCR, and Western blot analysis. Importantly, the protective effect of SHTL in the LPS- and Ox-LDL-induced macrophages against inflammation and lipid accumulation was attenuated by GW9662, a PPAR- antagonist, which confirmed the prediction results of network pharmacology. In summary, these results indicated that SHTL pretreatment reduced inflammation and lipid accumulation of macrophages by activating the PPAR-/LXR-/ABCA1 pathway, which may provide a new insight into the mechanism of SHTL in the suppression of AS progression.
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http://dx.doi.org/10.1155/2020/3426925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556105PMC
May 2021

Effect of Stressors on the Carrying Capacity of Spatially Distributed Metapopulations.

Am Nat 2020 08 10;196(2):E46-E60. Epub 2020 Jun 10.

Stressors such as antibiotics, herbicides, and pollutants are becoming increasingly common in the environment. The effects of stressors on populations are typically studied in homogeneous, nonspatial settings. However, most populations in nature are spatially distributed over environmentally heterogeneous landscapes with spatially restricted dispersal. Little is known about the effects of stressors in these more realistic settings. Here, we combine laboratory experiments with novel mathematical theory to rigorously investigate how a stressor's physiological effect and spatial distribution interact with dispersal to influence population dynamics. We prove mathematically that if a stressor increases the death rate and/or simultaneously decreases the population growth rate and yield, a homogeneous distribution of the stressor leads to a lower total population size than if the same amount of the stressor was heterogeneously distributed. We experimentally test this prediction on spatially distributed populations of budding yeast (). We find that the antibiotic cycloheximide increases the yeast death rate but reduces the growth rate and yield. Consistent with our mathematical predictions, we observe that a homogeneous spatial distribution of cycloheximide minimizes the total equilibrium size of experimental metapopulations, with the magnitude of the effect depending predictably on the dispersal rate and the geographic pattern of antibiotic heterogeneity. Our study has implications for assessing the population risk posed by pollutants, antibiotics, and global change and for the rational design of strategies for employing toxins to control pathogens and pests.
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http://dx.doi.org/10.1086/709293DOI Listing
August 2020

Comparative physiological mechanisms of arbuscular mycorrhizal fungi in mitigating salt-induced adverse effects on leaves and roots of Zelkova serrata.

Mycorrhiza 2020 May 9;30(2-3):341-355. Epub 2020 May 9.

Dafeng Forest Farm, Yancheng, 224136, Jiangsu, China.

Arbuscular mycorrhizal (AM) fungi enhance plant salt tolerance. However, physiological mechanisms of enhanced salt tolerance in leaves and roots of trees rarely have been compared. To reveal the different mechanisms, our study utilized comprehensive analyses of leaves and roots to examine the effects of Funneliformis mosseae on the salinity tolerance of Zelkova serrata. Seedlings of Z. serrata were exposed to four salt levels in a greenhouse with and without F. mosseae inoculation. Treatment comparisons revealed that following F. mosseae inoculation, (1) nutrient deficiency caused by osmotic stress was mitigated by the fungus enhancing nutrient contents (K, Ca, and Mg) in roots and (N, P, K, Ca, and Mg) in leaves, with Ca and K contents being higher in both leaves and roots; (2) mycorrhizas alleviated ion toxicity by maintaining a favorable ion balance (e.g., K/Na), and this regulatory effect was higher in leaves than that in roots; and (3) oxidative damage was reduced by an increase in the activities of antioxidant enzymes and accumulation of antioxidant compounds in mycorrhizal plants although the increase differed in leaves and roots. In particular, AM fungus-enhanced catalase activity and reduced glutathione content only occurred in leaves, whereas an enhanced content of reduced ascorbic acid was only noted in roots. Growth, root vitality, leaf photosynthetic pigments, net photosynthetic rate, and dry weight were higher in seedlings with AM fungus inoculation. These results suggest that AM fungus inoculation improved salinity tolerance of Z. serrata, but the physiological mechanisms differed between leaves and roots.
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http://dx.doi.org/10.1007/s00572-020-00954-yDOI Listing
May 2020

Effect of Aerobic and Resistance Training on Endothelial Progenitor Cells in Mice with Type 2 Diabetes.

Cell Reprogram 2020 08 21;22(4):189-197. Epub 2020 Apr 21.

Department of Nursing, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Since no study has explored whether exercise could improve impaired proliferation, migration, and angiogenesis of endothelial progenitor cells (EPCs) in animal models or humans with type 2 diabetes, we aimed to explore the effect of different models of exercise on EPC function and expression of caveolin-1, PI3K, and AKT in mice with type 2 diabetes. Male db/db mice (age: 8 weeks) with type 2 diabetes were subjected to aerobic training (AT), resistance training (RT), or combined aerobic and resistance training (AT+RT) 3 or 4 days/week. Mice in the control group remained sedentary with no specific training requirement. Bone marrow-derived EPCs were isolated, and the protein concentrations of caveolin-1, Pi3k, and AKT, and EPC function, were identified in the 1st, 4th, 8th, and 12th weeks of the intervention. Greater increases in proliferation, migration, and angiogenesis were observed in the AT, RT, and AT+RT groups than in the control group. AT+RT was more effective than AT or RT in improving the migratory and angiogenesis function of EPCs in mice with type 2 diabetes and achieved maximum improvement after 8 weeks of intervention. Western blot analysis showed that caveolin-1, p-PI3k, and p-Akt levels were obviously increased in the AT, RT, and AT+RT groups compared with the control group. The expression level of these proteins in the AT+RT group was higher than that in the AT and RT groups. AT+RT may be a helpful reference when choosing exercise methods for the prevention of diabetes-related cardiovascular diseases.
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http://dx.doi.org/10.1089/cell.2019.0063DOI Listing
August 2020

Identification of CD4 T cell epitopes on glyceraldehyde-3-phosphate dehydrogenase-C of Staphylococcus aureus in Babl/c mice.

Microb Pathog 2020 Jul 25;144:104167. Epub 2020 Mar 25.

College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, 163319, China; College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, 163319, China. Electronic address:

Glyceraldehyde-3-phosphate dehydrogenase-C (GapC) is a highly conserved surface protein of Staphylococcus aureus, with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity, which represents an excellent vaccine candidate antigen. It can induce protective immune responses to S. aureus infections. However, CD4 T cell epitopes of GapC that induce CD4 T cell immune responses are currently unclear. In this study, we used bioinformatics prediction algorithms to predict CD4 T cell epitopes of GapC. Ten peptides were synthesized to investigate the candidate epitopes. Our results showed that the peptides, G4 (GapC ) and G10 (GapC were able to induce proliferation of CD4 T cells and secrete high levels of interferon (IFN)-γ, respectively. In addition, they significantly reduced bacterial loads in tissue and induced immunoprotective effects. It is suggested that G4 and G10 are Th1-type epitopes of S. aureus GapC. This study provides the potential development of the design of epitope-based vaccine against S. aureus.
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http://dx.doi.org/10.1016/j.micpath.2020.104167DOI Listing
July 2020

Comparative effects of the recovery from sulfuric and nitric acid rain on the soil enzyme activities and metabolic functions of soil microbial communities.

Sci Total Environ 2020 Apr 18;714:136788. Epub 2020 Jan 18.

Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Province Key Laboratory of Soil and Water Conservation and Ecological Restoration, Nanjing Forestry University, 159 Longpan Road, Nanjing, Jiangsu 210037, China. Electronic address:

Acid rain (AR) is a serious issue in China, particularly in the Yangtze River Delta region where the economy has undergone rapid development. Over the last few years, the composition of acid rain in the Yangtze River Delta region has gradually changed from sulfuric acid rain (SAR) to nitric acid rain (NAR) due to controls on SO emissions, but increased NO emissions. These changes have made ecosystems more complex. For this study, we halted AR treatments in Quercus acutissima forest plots that had received simulated AR for one year and monitored them from the following February to November. We investigated their soil resident enzyme and microbial metabolic activities, as well as community functional diversity. The results revealed that AR treatments negatively affected both the soil microbial activity and soil microbial community functional diversity; however, both managed to recover over time, once the AR treatments were stopped. During the AR treatment and recovery periods, four main categories (carbohydrates, carboxylic acids, amino acids, and polymers) were dominantly utilized. The utilization of pyruvic acid, which was affected by the AR treatments, as well as d-mannitol and tween 80, accounted for changes in the peak values of the C substrate groups during the AR treatment recovery period. Finally, changes in the activities of soil enzymes recorded following AR recovery, were closely related to the utilization of six C substrate groups. Our results suggested that the recovery of soils following the cessation of NAR stress was more rapid than from SAR. Further, that short-term NAR could be easily treated during the transformation from SAR to NAR in the Yangtze River Delta region. These results might also enrich the basic data relating to post-AR treatments on the soil environment, while having significance toward guiding further studies on the recovery of ecosystems from AR.
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http://dx.doi.org/10.1016/j.scitotenv.2020.136788DOI Listing
April 2020

Ginsenoside Rd attenuates ACTH-induced corticosterone secretion by blocking the MC2R-cAMP/PKA/CREB pathway in Y1 mouse adrenocortical cells.

Life Sci 2020 Mar 20;245:117337. Epub 2020 Jan 20.

Research Center of Traditional Chinese Medicine, the Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China. Electronic address:

Background: Higher levels of glucocorticoids (GCs), and impaired regulation of the hypothalamic-pituitary-adrenal (HPA) axis may cause or exacerbate the occurrence of metabolic and psychiatric disorders. It has been reported that ginseng saponin extract (GSE) has an inhibitory effect on the hyperactivity of the HPA axis induced by stresses and increased corticosterone level induced by intraperitoneal injection of adrenocorticotrophic hormone (ACTH) in mice. However, the molecular mechanisms by which GSE and its active ginsenosides inhibit corticosterone secretion remain elusive.

Main Methods: Y1 mouse adrenocortical cells were treated with ACTH for up to 60 min to establish a cell model of corticosterone secretion. After treatment with different concentrations of GSE or ginsenoside monomers for 24 h prior to the addition of ACTH, analyses of cAMP content, PKA activity, and the levels of steroidogenesis regulators, melanocortin-2 receptor (MC2R), and melanocortin-2 receptor accessory protein (MRAP) in ACTH-induced Y1 cells were performed.

Results: We demonstrated that GSE inhibits ACTH-stimulated corticosterone production in Y1 cells by inhibiting factors critical for steroid synthesis. Ginsenoside Rd, an active ingredient of GSE, inhibits corticosterone secretion in the cells and impedes ACTH-induced corticosterone biosynthesis through down-regulation of proteins in the cAMP/PKA/CREB signaling pathway. In addition, Western blot and qPCR analyses showed that ginsenoside Rd attenuated the induction of MC2R and MRAP by ACTH.

Conclusion: Our findings indicate that ginsenoside Rd inhibits ACTH-induced corticosterone production through blockading the MC2R-cAMP/PKA/CREB pathway in adrenocortical cells. Overall, this mechanism may represent an important therapeutic option for the treatment of stress-related disorders, further supporting the pharmacological benefits of ginseng.
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http://dx.doi.org/10.1016/j.lfs.2020.117337DOI Listing
March 2020

Aerobic and resistance training enhances endothelial progenitor cell function via upregulation of caveolin-1 in mice with type 2 diabetes.

Stem Cell Res Ther 2020 01 3;11(1):10. Epub 2020 Jan 3.

Department of Endocrinology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.

Background: To explore the effect of aerobic training (AT), resistance training (RT) or a combination of AT and RT (AT+RT) on the function of endothelial progenitor cells (EPCs) in mice with type 2 diabetes and the potential effective mechanisms METHODS: Eight-week-old db/db male mice were used as type 2 diabetic animal models in this study. Mice were randomly assigned to the control group (n = 5), AT group (n = 5), RT group (n = 5) and AT+RT group (n = 5). Mice in the control group remained sedentary with no specific training requirement. Mice were motivated to perform AT, RT or AT+RT by a gentle pat on their body for 3 or 4 days/week for 14 days. AT was performed by treadmill running, RT was performed by ladder climbing and AT+RT involved both AT and RT. Bone-derived EPCs were isolated after 14 days of the intervention. EPC expression of CD31, CD34, CD133, CD144 and VEGFR2 was detected by immunofluorescence staining. Fluorescence detection was performed on attached mononuclear cells to detect double-positive EPCs. We then explored the effect of caveolin-1 knockdown (lentiviral vector with caveolin-1-siRNA) on the proliferation and adherence of EPCs and the concentration of caveolin-1 and PI3K/AKT via western blot analyses.

Results: Compared to the mice in the control group, the mice in the AT, RT and AT+RT groups presented significant increases in proliferation and adherence after 14 days of intervention. AT+RT induced an increase in EPC adherence, which was greater than that of the control, RT and AT groups. Caveolin-1 knockdown inhibited the EPC proliferative and adherent abilities. The AT+RT group showed higher levels of caveolin-1 and p-AKT than the control group, but these changes were decreased by caveolin-1-siRNA transfection.

Conclusion: Combined AT and RT is an effective way to improve EPC function through upregulation of caveolin-1 in mice with type 2 diabetes.
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http://dx.doi.org/10.1186/s13287-019-1527-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942272PMC
January 2020

Optimization and Identification of Antioxidant Peptide from Underutilized : Extraction, Gastrointestinal Digestion, and Fractionation.

Biomed Res Int 2019 20;2019:6424651. Epub 2019 Aug 20.

Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, China.

DPPH• scavenging peptides (<3kDa) from underutilized protein were obtained by the following successive treatment, i.e., ultrasound extraction, simulated gastrointestinal digestion hydrolyzation, and membrane ultrafiltration classification. The optimal condition for ultrasound-assisted extraction was an ultrasound wave with 800 W of power treating a mixture of 60 mL of 1.0 mol L NaOH and 2 g algae powder for 15 min. A high correlation (r=0.8146) between DPPH• scavenging activity and yield of the intact peptides showed their antioxidant capacity. Simulated digestion assay resulted in excellent DPPH• scavenging activity of the total peptide, amounting to (86.5 ± 10.1)%, comparing with the nondigestion samples at (46.8 ± 6.5)%. After fractionation, the 500-1000 Da fraction exhibited the highest DPPH• scavenging activity (81.2 ± 4.0)%, increasing 1.5 times due to digestion. Then, the 500-1000 Da fraction was analyzed by RPLC-Q Exactive HF mass spectrometer, and 4 novel peptides, i.e., Ile-Leu-Thr-Lys-Ala-Ala-Ile-Glu-Gly-Lys, Ile-Ile-Tyr-Phe-Gln-Gly-Lys, Asn-Asp-Pro-Ser-Thr-Val-Lys, and Thr-Val-Arg-Pro-Pro-Gln-Arg, were identified. From these amino acid sequences, hydrophobic residues accounted for 56%, which indicated their high antioxidant property. The results indicated that underutilized protein of could be a potential source of antioxidative peptides through simulated gastrointestinal digestion.
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http://dx.doi.org/10.1155/2019/6424651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720044PMC
February 2020

High quantum yield pure blue emission and fast proton conduction from an indium-metal-organic framework.

Dalton Trans 2019 Aug 25;48(32):12088-12095. Epub 2019 Jul 25.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry & Molecular Engineering, Nanjing Tech University, Nanjing 211816, P. R. China. and State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China. and College of Materials Science & Engineering, Nanjing Tech University, Nanjing 211816, P. R. China.

A multifunctional material, with two or more types of functionalities in a single material, might give new phenomena and novel applications. Metal-organic frameworks (MOFs) or porous coordination polymers (PCPs) can be utilized as a highly versatile and tunable platform for exploring multifunctional materials. In this study, an extra thermally stable indium-MOF (1) was synthesized via self-assembly of 1,1'-ethynebenzene-3,3',5,5'-tetracarboxylic acid (HEBTC) with an indium salt under solvothermal conditions. MOF 1 crystallizes in a tetragonal space group P4/mmm and shows a three-dimensional (3D) anionic framework with one-dimensional (1D) channels along the c-axis direction, and charge-balancing [(CH)NH] cations together with lattice DMF and water molecules are accommodated in channels. This multifunctional MOF emits pure blue luminescence under ambient conditions, arising from the π* →π electron transition in EBTC, with CIE coordinates of (0.16, 0.07), and shows the highest quantum yield of 61.4% among indium-MOFs. Alternatively, 1 features fast proton conductivity with σ = 3.49 × 10 S cm at 25 °C and 99% RH, attributed to the efficiently synergistic proton transfer in the H-bond networks constructed by [NH(CH)] cations, lattice water molecules in channels and carboxylate groups in the framework. The notable level of performance of 1, combined with good hydrolytic and extra thermal stability, offers great opportunities for its future applications, and it may behave as a kind of promising multifunctional MOF material.
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http://dx.doi.org/10.1039/c9dt02472gDOI Listing
August 2019

Two-year-supervised resistance training prevented diabetes incidence in people with prediabetes: A randomised control trial.

Diabetes Metab Res Rev 2019 07 5;35(5):e3143. Epub 2019 Mar 5.

Jiangsu Province Hospital on Integration of Chinese and Western Medicine Affiliated with Nanjing University of Chinese Medicine, Nanjing, China.

Aim: The purpose of this study is to explore the long-term effects of aerobic training (AT), resistance training (RT), and combined training (AT + RT) on the prevention of T2D incidence in patients with prediabetes.

Materials And Methods: In this randomised controlled trial, people with prediabetes (fasting glucose ≥5.6 and <7.0 mmol/L and/or 2-h glucose ≥7.8 and <11.1 mmol/L on the 75-g oral glucose tolerance test and/or haemoglobin A ≥5.7% and <6.4%) were randomly assigned to the control group, AT group, RT group, or AT + RT group. Supervised exercise programmes, including AT, RT, and AT + RT, were completed for 60 minutes per day, three non-consecutive days per week for 24 months. The primary outcome was the incidence of T2D; secondary outcomes were blood glucose and lipid levels, including total cholesterol (TC) and standard 2-hour oral glucose tolerance (2hPG).

Results: A total of 137 (80%) subjects with a mean age of 59 years (45 men, 92 women) entered the final analysis. After 24 months of intervention, the incidences of T2D adjusted by sex and age were significantly decreased by 74% (95% CI, 38-89), 65% (95% CI, 21-85), and 72% (95% CI, 36-87) in the AT + RT, RT, and AT groups compared with the control group (HR: AT + RT 0.26 [95% CI, 0.11-0.62], RT 0.35 [95% CI, 0.15-0.79], and AT 0.28 [95% CI, 0.13-0.64]). The cumulative T2D incidences were significantly lower in the AT + RT, RT, and AT groups than in the control group (21%, 26%, and 22% vs 69%). The blood glucose and lipid profiles improved more in the AT, RT, and AT + RT groups than in the control group.

Conclusion: RT and RT plus AT were as effective as isolated AT in preventing progression to T2D.
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http://dx.doi.org/10.1002/dmrr.3143DOI Listing
July 2019

Assessment of cholestasis in drug-induced liver injury by different methods.

Medicine (Baltimore) 2019 Feb;98(6):e14399

Xiang Yang No 1 Peoples Hospital, Hubei Xiangyang, China.

Cholestasis in drug-induced liver injury (DILI) can be assessed by biochemical and pathologic methods, but the agreement between the 2 methods remains unclear.The aim of this study was to identify the accurate method for assessment of cholestasis in DILI.The DILI standard established and revised by the Council for International Organizations of Medical Sciences (CIOMS) (R values were calculated by liver function at different time points), cholestatic liver disease guideline (European Association for the Study of the Liver, EASL), and liver pathology were used to assess, compare, and analyze the cholestasis in 133 patients with DILI.The R values at different time points in CIOMS standard had no statistical difference for the assessment of cholestatic DILI (a = 0.05, χ = 1.51, P = .679). There were statistical differences among the results of CIOMS, EASL, and pathology (a = 0.05, χ = 99.97, P < .001). EASL standard had no statistical difference with pathology (a = 0.003, χ = 8.00, P = .005).CIOMS and EASL standards based on biochemical parameters underestimated cholestatic DILI, as compared to liver pathology.
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http://dx.doi.org/10.1097/MD.0000000000014399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6380790PMC
February 2019

Efficient Hydrolysis of Raw Microalgae Starch by an α-Amylase (AmyP) of Glycoside Hydrolase Subfamily GH13_37.

J Agric Food Chem 2018 Dec 26;66(48):12748-12755. Epub 2018 Nov 26.

Anhui Key Laboratory of Modern Biomanufacturing, School of Life Sciences, School of Resources and Environmental Engineering , Anhui University , Hefei 230601 , Anhui P.R. China.

Microalgae starch is receiving increasing attention as a renewable feedstock for biofuel production. Raw microalgae starch from Tetraselmis subcordiformis was proven to be very efficiently hydrolyzed by an α-amylase (AmyP) of glycoside hydrolase subfamily GH13_37 below the temperature of gelatinization (40 °C). The hydrolysis degree reached 74.4 ± 2.2% for 4% raw microalgae starch and 53.2 ± 1.7% for 8% raw microalgae starch after only 2 h. The hydrolysis efficiency was significantly stimulated by calcium ions. The enzyme catalysis of AmyP and its mutants (Q306A and E347A) suggested that calcium ions contributed to the hydrolysis of cyclic structures in raw microalgae starch by a distinctive calcium-binding site Ca2 of AmyP. The study explored raw microalgae starch as a new resource for cold enzymatic hydrolysis and extended our knowledge on the function of calcium in amylolytic enzyme.
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http://dx.doi.org/10.1021/acs.jafc.8b03524DOI Listing
December 2018

Correction to: Reassessing Coronary Artery Bypass Surgery Versus Percutaneous Coronary Intervention in Patients with Type 2 Diabetes Mellitus: A Brief Updated Analytical Report (2015-2017).

Diabetes Ther 2018 12;9(6):2407

Institute of Cardiovascular Diseases and Guangxi Key Laboratory Base of Precision Medicine in Cardio-cerebrovascular Disease Control and Prevention and Guangxi Clinical Research Center for Cardio-cerebrovascular Diseases, The First Affiliated Hospital of Guangxi Medical University, Guangxi, China.

In the original publication, conclusion was incorrectly updated in the article main text. The complete statement is given below.
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http://dx.doi.org/10.1007/s13300-018-0520-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6250634PMC
December 2018

Metal ion coordination enhancing quantum efficiency of ligand phosphorescence in a double-stranded helical chain coordination polymer of Pb with nicotinic acid.

Dalton Trans 2018 Oct;47(41):14636-14643

State Key Laboratory of Materials-Oriented Chemical Engineering and College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 210009, P. R. China.

A new 1D phosphorescence coordination polymer (CP) [Pb2O(C6H4NO2)2]n (1; C6H4NO2 = nicotinate) was synthesized by a solvothermal reaction and PbO was used as a Pb(ii) source instead of traditional Pb(ii) salts. This remarkably thermal-stable CP crystallizes in the space group I41/a. In the crystal structure of 1, two different Pb(ii) ions show a five-coordinated and hemidirected coordination geometry, two nonequivalent nicotinate ligands link to Pb(ii) ions in μ2-η1:η1 and μ4-η2:η2 modes, and the hemidirected coordination polyhedra of Pb(ii) form a helical lead-oxide chain via an edge-sharing fashion along the c-axis. Under ambient conditions, 1 emits cyan ligand-based phosphorescence with an absolute quantum yield as high as 59.4% and a lifetime of 9.86 ms under UV-light irradiation. Under the same conditions, nicotinic acid emits simultaneously fluorescence and phosphorescence with a total absolute quantum yield of 4.8%. The great enhancement of phosphorescence quantum yield in 1, regarding nicotinic acid, is assigned to the heavy atom effect of Pb(ii) and negligible ππ interaction between pyridyl rings. Noticeably, the vibronic fine structure is observed in the emission spectrum of 1 at room temperature. Additionally, 1 shows thermochromic behavior, and such functionality probably has realistic application in the field of temperature detection.
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http://dx.doi.org/10.1039/c8dt03589jDOI Listing
October 2018

H- and J-aggregates formed from a nontraditional π-gelator depending on the solvent polarity for the detection of amine vapors.

Org Biomol Chem 2018 10;16(40):7438-7445

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, P.R. China.

A tert-butyl carbazole-modified difluoroboron β-diketonate complex (TCbzB) has been synthesized. Although no traditional gelation group was involved in TCbzB, it could form organogels in the mixed solvents of o-dichlorobenzene/cyclohexane (v/v = 1/5 or 1/2), toluene/cyclohexane (v/v = 1/2) and chlorobenzene/cyclohexane (v/v = 1/2). Interestingly, an orange organogel was obtained in o-dichlorobenzene/cyclohexane (v/v = 1/2) with relatively high polarity and red organogels were gained in the other three mixed solvents with relatively low polarity. TCbzB self-assembled into H-aggregates and J-aggregates in orange and red organogels, respectively, and the corresponding xerogels emitted yellow and red light, respectively, under UV illumination. The red emission of the xerogel-based film could be quenched significantly by gaseous n-propylamine and aniline because of the decomplexation of the difluoroboron β-diketonate complex by n-propylamine and the weak interactions between aniline and boron difluoride units.
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http://dx.doi.org/10.1039/c8ob01596aDOI Listing
October 2018

Reassessing Coronary Artery Bypass Surgery Versus Percutaneous Coronary Intervention in Patients with Type 2 Diabetes Mellitus: A Brief Updated Analytical Report (2015-2017).

Diabetes Ther 2018 Oct 15;9(5):2163-2171. Epub 2018 Sep 15.

Institute of Cardiovascular Diseases and Guangxi Key Laboratory Base of Precision Medicine in Cardio-cerebrovascular Disease Control and Prevention and Guangxi Clinical Research Center for Cardio-cerebrovascular Diseases, The First Affiliated Hospital of Guangxi Medical University, Guangxi, China.

Introduction: In this analysis, we aimed to systematically compare percutaneous coronary intervention (PCI) versus coronary artery bypass surgery (CABG) in terms of adverse outcomes utilizing data from a recent (2015-2017) population of patients with type 2 diabetes mellitus (T2DM).

Methods: An electronic search of recent studies (2015-2017) was carried out using 'diabetes mellitus,' 'coronary artery bypass surgery,' and 'percutaneous coronary intervention' as the main search terms. Uncomplicated T2DM patients with stable coronary artery disease (CAD), left main CAD, and multi-vessel disease were included. RevMan software (version 5.3) was used to calculate odds ratios (OR) and 95% confidence intervals (CIs).

Results: Among a total of 13,114 T2DM patients, CABG and PCI patients did not differ significantly in their rates of mortality (OR 0.90, 95% CI 0.61-1.31; P = 0.57) and cardiac death (OR 1.00, 95% CI 0.78-1.30; P = 0.98). However, rates of major adverse events, repeat revascularization, and myocardial infarction were significantly higher in the PCI group. Stroke rates did not significantly differ between the two groups.

Conclusion: Mortality (1-5 years) did not significantly differ between the CABG and PCI patients with T2DM. However, rates of other major adverse events were significantly higher in the PCI patients, suggesting that CABG is more advantageous than PCI in patients with T2DM.
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http://dx.doi.org/10.1007/s13300-018-0504-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167293PMC
October 2018

Does a Nurse-Led Program of Support and Lifestyle Management for patients with coronary artery disease significantly improve psychological outcomes among the patients?: A meta-analysis.

Medicine (Baltimore) 2018 Aug;97(35):e12171

Department of Internal Medicine Education Department of Endocrinology, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Background: Nowadays, secondary prevention of coronary heart disease (CHD) is commonly provided by nurse-coordinated prevention programs (NCPPs). NCPPs were recommended to be incorporated into the healthcare systems by the European Society of Cardiology (ESC) as stated in their 2012 European Guideline. Even if Nurse-Led Programs of Support and Lifestyle Management are beneficial to the patients with CHD, it is not clear whether these programs significantly improve psychological outcomes among the patients. Therefore, in this analysis, we aimed to systematically compare anxiety and depression reported among CHD patients who were assigned to a Nurse-Led Programs of Support and Lifestyle Management versus patients who were assigned to a normal usual care setting.

Methods: Online databases were searched for English publications assessing anxiety and depression in CHD patients who were assigned to a Nurse Interventional program versus patients who were assigned to a normal usual care setting. This analysis was carried out by RevMan software (version 5.3). For dichotomous data, odds ratios (ORs) and 95% confidence intervals (CIs) were generated whereas for continuous data, weight mean difference (WMDs) and 95% CIs were calculated.

Results: A total number of 3110 patients were analyzed (1526 participants were assigned to the Nurse Interventional group whereas 1584 participants were assigned to the normal usual care group). Patients' enrollment time period varied from the year 2008 to the year 2015. Results of this analysis showed that depression among participants who were assigned to a Nurse-Led Program of Support and Lifestyle Management was not significantly different (OR: 0.90, 95% CI: 0.68-1.20; P = .47) compared to participants who were assigned to the normal usual care setting. When continuous data were used, still no significant difference was observed (WMD: -0.83, 95% CI: -1.68-0.02; P = .06). A similar result was obtained even when anxiety was assessed (WMD: -1.38, 95% CI: -3.21-0.45; P = .14).

Conclusions: The current analysis did not show any significant improvement in reduction of depression and anxiety among CHD patients who were assigned to a Nurse-Led Program of Support and Lifestyle Management versus those patients who were assigned to a normal usual care setting. Therefore, according to this analysis, even if a Nurse-Led Program of Support and Lifestyle Management might be clinically effective, it does not improve mental well-being in these patients with CHD.
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http://dx.doi.org/10.1097/MD.0000000000012171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6392672PMC
August 2018
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