Publications by authors named "Lu Xue"

208 Publications

Identification of Underlying Hub Genes Associated with Hypertrophic Cardiomyopathy by Integrated Bioinformatics Analysis.

Pharmgenomics Pers Med 2021 12;14:823-837. Epub 2021 Jul 12.

Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, 310016, People's Republic of China.

Background: Considered as one of the major reasons of sudden cardiac death, hypertrophic cardiomyopathy (HCM) is a common inherited cardiovascular disease. However, effective treatment for HCM is still lacking. Identification of hub gene may be a powerful tool for discovering potential therapeutic targets and candidate biomarkers.

Methods: We analysed three gene expression datasets for HCM from the Gene Expression Omnibus. Two of them were merged by "sva" package. The merged dataset was used for analysis while the other dataset was used for validation. Following this, a weighted gene coexpression network analysis (WGCNA) was performed, and the key module most related to HCM was identified. Based on the intramodular connectivity, we identified the potential hub genes. Then, a receiver operating characteristic curve analysis was performed to verify the diagnostic values of hub genes. Finally, we validated changes of hub genes, for genetic transcription and protein expression levels, in datasets of HCM patients and myocardium of transverse aortic constriction (TAC) mice.

Results: In the merged dataset, a total of 455 differentially expressed genes (DEGs) were identified from normal and hypertrophic myocardium. In WGCNA, the blue module was identified as the key module and the genes in this module showed a high positive correlation with HCM. Functional enrichment analysis of DEGs and key module revealed that the extracellular matrix, fibrosis, and neurohormone pathways played important roles in HCM. FRZB, COL14A1, CRISPLD1, LUM, and sFRP4 were identified as hub genes in the key module. These genes showed a good predictive value for HCM and were significantly up-regulated in HCM patients and TAC mice. We also found protein expression of LUM and sFRP4 increased in myocardium of TAC mice.

Conclusion: This study revealed that five hub genes are involved in the occurrence and development of HCM, and they are potentially to be used as therapeutic targets and biomarkers for HCM.
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http://dx.doi.org/10.2147/PGPM.S314880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285300PMC
July 2021

Cytogenetic monitoring of peripheral blood lymphocytes from medical radiation professionals occupationally exposed to low-dose ionizing radiation.

Mutat Res 2021 Jul 5;867:503370. Epub 2021 Jun 5.

China CDC Key Laboratory of Radiation Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing, 100088, PR China. Electronic address:

In order to assess the health risk of low-dose radiation to radiation professionals, monitoring is performed through chromosomal aberration analysis and micronuclei (MN) analysis. MN formation has drawbacks for monitoring in the low-dose range. Nucleoplasmic bridge (NPB) analysis, with a lower background level, has good dose-response relationships at both high and relatively low dose ranges. Dicentric and ring chromosomes were analyzed in 199 medical radiation professionals, and NPB/MN yields were analyzed in 205 radiation professionals. The effects of sex, age of donor, types of work, and length of service on these cytogenetic endpoints were also analyzed. The yields of the three cytogenetic endpoints were significantly higher in radiation professionals versus controls. Frequencies of dicentric plus ring chromosomes were affected by length of service. NPB frequencies were influenced by type of work and length of service. MN yields were affected not only by types of work and length of service but also by donor sex and age. In conclusion, dicentric plus ring chromosomes, NPB, and MN can be induced by low-dose radiation in radiation professionals. NPB is a potential biomarker to assess the health risk of occupational low-dose radiation exposure.
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http://dx.doi.org/10.1016/j.mrgentox.2021.503370DOI Listing
July 2021

[Generation and phenotypic characterization of S100A9 gene knockout mice by CRISPR/Cas9-mediated gene targeting].

Sheng Li Xue Bao 2021 Jun;73(3):482-490

Institute of Medical Biology, College of Life Sciences, South-Central University for Nationalities, Wuhan 430072, China.

S100 calcium binding protein A9 (S100A9) is involved in a variety of biological processes such as inflammation and tumor cell migration and invasion regulation. The purpose of this study was to construct S100A9 gene-edited mice by using CRISPR/Cas9 technology, thereby providing an animal model for exploring the biological functions of this gene. According to the S100A9 gene sequence, the single-stranded small guide RNA (sgRNA) targeting exons 2 and 3 was transcribed in vitro, and a mixture of Cas9 mRNA and candidate sgRNA was injected into mouse fertilized eggs by microinjection. Early embryos were obtained and transferred to surrogate mice, and F mice were obtained and identified by PCR identification and gene sequencing. F mice were further mated with wild-type C57BL/6 mice to obtain F heterozygous mice, and then homozygous offspring were obtained through F mice self-crossing. Real-time PCR, Western blot and immunohistochemistry (IHC) were used to verify the expression and distribution of S100A9. In order to observe the pathological changes of mouse lung tissue using HE staining, an allergic asthma model was induced by ovalbumin from chicken egg white (OVA). The results showed that the 2 492 bp of exons 2, 3 of the S100A9 gene was successfully knocked out, and S100A9 mice with stable inheritance were obtained. Furthermore, it was found that S100A9 gene was highly expressed in the lung and spleen of wild-type mice. The expression of S100A9 mRNA and protein was not detected in the lung and spleen of S100A9 mice. However, compared with wild-type mice, the lungs of S100A9 mice showed a significantly worse inflammatory phenotype, and the proportion of eosinophils in bronchoalveolar lavage fluid (BALF) was significantly increased in response to the treatment of OVA. These results suggest we have successfully constructed a new strain of S100A9 mice, and preliminarily confirmed that the lack of S100A9 function can aggravate airway inflammation in asthmatic mice, providing a new mouse model for further study of S100A9 gene function.
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June 2021

Protein arginine methyltransferase 1 regulates cell proliferation and differentiation in adult mouse adult intestine.

Cell Biosci 2021 Jun 22;11(1):113. Epub 2021 Jun 22.

Section on Molecular Morphogenesis, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), National Institutes of Health (NIH), Bethesda, MD, 20892, USA.

Background: Adult stem cells play an essential role in adult organ physiology and tissue repair and regeneration. While much has been learnt about the property and function of various adult stem cells, the mechanisms of their development remain poorly understood in mammals. Earlier studies suggest that the formation of adult mouse intestinal stem cells takes place during the first few weeks after birth, the postembryonic period when plasma thyroid hormone (T3) levels are high. Furthermore, deficiency in T3 signaling leads to defects in adult mouse intestine, including reduced cell proliferation in the intestinal crypts, where stem cells reside. Our earlier studies have shown that protein arginine methyltransferase 1 (PRMT1), a T3 receptor coactivator, is highly expressed during intestinal maturation in mouse.

Methods: We have analyzed the expression of PRMT1 by immunohistochemistry and studied the effect of tissue-specific knockout of PRMT1 in the intestinal epithelium.

Results: We show that PRMT1 is expressed highly in the proliferating transit amplifying cells and crypt base stem cells. By using a conditional knockout mouse line, we have demonstrated that the expression of PRMT1 in the intestinal epithelium is critical for the development of the adult mouse intestine. Specific removal of PRMT1 in the intestinal epithelium results in, surprisingly, more elongated adult intestinal crypts with increased cell proliferation. In addition, epithelial cell migration along the crypt-villus axis and cell death on the villus are also increased. Furthermore, there are increased Goblet cells and reduced Paneth cells in the crypt while the number of crypt base stem cells remains unchanged.

Conclusions: Our finding that PRMT1 knockout increases cell proliferation is surprising considering the role of PRMT1 in T3-signaling and the importance of T3 for intestinal development, and suggests that PRMT1 likely regulates pathways in addition to T3-signaling to affect intestinal development and/or homeostasis, thus affecting cell proliferating and epithelial turn over in the adult.
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http://dx.doi.org/10.1186/s13578-021-00627-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220849PMC
June 2021

Systematic metabolic tools reveal underlying mechanism of product biosynthesis in Chromochloris zofingiensis.

Bioresour Technol 2021 Oct 14;337:125406. Epub 2021 Jun 14.

Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China; Shenzhen Key Laboratory of Marine Microbiome Engineering, Shenzhen University, Shenzhen 518060, China. Electronic address:

This study comprehensively explored underlying mechanism of fed-batch culture on product biosynthesis in Chromochloris zofingiensis by dynamic model, targeted metabolite determination, enzyme activity analysis, and C tracer-based metabolic flux analysis. Based on dynamic models of cell growth and product formation, exponential fed-batch culture and fed-batch culture based on pH changes were established to increase biomass concentration by 20.05-fold and 18.28-fold, respectively. Exponential fed-batch culture exhibited great potentials in biodiesel and protein productions from microalgae. Systematic metabolic tools revealed fed-batch culture limited photosynthetic efficiency by inhibiting photosystem and Rubisco activity, while strengthened respiratory action to provide more substances and energy for product biosynthesis. Fed-batch culture elevated biosynthetic capability for carotenoid and lipid by promoting related metabolic flux and contents of pyruvate and ace-CoA. Finally, economic analysis revealed biomass cost was decreased to 1.99 $/kg from 2.39 $/kg, suggesting fed-batch culture was a cost-effective strategy to improve economic viability of microalgal production.
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http://dx.doi.org/10.1016/j.biortech.2021.125406DOI Listing
October 2021

Engineering Platinum-Cobalt Nano-alloys in Porous Nitrogen-Doped Carbon Nanotubes for Highly Efficient Electrocatalytic Hydrogen Evolution.

Angew Chem Int Ed Engl 2021 Jun 17. Epub 2021 Jun 17.

School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Singapore.

Highly efficient electrocatalysts are essential for the production of green hydrogen from water electrolysis. Herein, a metal-organic framework-assisted pyrolysis-replacement-reorganization approach is developed to obtain ultrafine Pt-Co alloy nanoparticles (sub-10 nm) attached on the inner and outer shells of porous nitrogen-doped carbon nanotubes (NCNT) with closed ends. During the thermal reorganization, the migration of Pt-Co nano-alloys to both surfaces ensures the maximized exposure of active sites while maintaining the robust attachment to the porous carbon matrix. Density functional theory calculations suggest a nearly thermodynamically-neutral free energy of adsorption for hydrogen intermediates and diversified active sites induced by alloying, thus resulting in a great promotion in intrinsic activity towards the hydrogen evolution reaction (HER). Benefiting from the delicate structural design and compositional modulation, the optimized Pt [email protected] electrocatalyst manifests outstanding HER activity and superior stability in both acidic and alkaline media.
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http://dx.doi.org/10.1002/anie.202106547DOI Listing
June 2021

Electroacupuncture alleviates spatial memory deficits in METH withdrawal mice by enhancing astrocyte-mediated glutamate clearance in the dCA1.

Addict Biol 2021 Jun 14:e13068. Epub 2021 Jun 14.

Department of Human Anatomy and Histoembryology, Nanjing University of Chinese Medicine, Nanjing, China.

Methamphetamine (METH) elicits endogenous glutamate (Glu) in the brain, which could partially explain METH-induced memory deficits. Here, we investigated the therapeutic effects of electroacupuncture (EA) on spatial memory deficits in METH withdrawal mice and its potential synaptic mechanisms. We found that EA at acupoints 'Baihui' and 'Yintang' ameliorated the impaired spatial memory in METH withdrawal mice. In parallel, EA attenuated the Glu levels in vivo and suppressed the neuronal activities within dCA1 of METH withdrawal mice, as indicated by the decreasing c-Fos levels and the amplitude of mEPSP. In the dCA1, EA decreased A1-like astrocytes but increased astrocytic glutamatergic transporting molecules including glutamate transporter 1 and glutamine synthase. However, EA seemed to have no effects on presynaptic Glu transmission from the dCA3, as evidenced by the similiar levels of c-Fos in the dCA3 neurons, synaptic vesicular markers of dCA3 neural terminals and values of paired-pulse ratio in the dCA1 neurons between EA-treated and sham EA-treated METH withdrawal mice. These findings suggest that EA might normalize the dCA1 Glu levels at least in part through enhancing astrocyte-mediated Glu clearance. Taken together, astrocytes might be a novel target for developing therapeutic interventions against the impaired memory behaviours in METH users, and EA represents a promising non-invasive therapeutic strategy for the management of drug-caused memory deficits.
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http://dx.doi.org/10.1111/adb.13068DOI Listing
June 2021

Proteomic Analysis Reveals That Placenta-Specific Protein 9 Inhibits Proliferation and Stimulates Motility of Human Bronchial Epithelial Cells.

Front Oncol 2021 28;11:628480. Epub 2021 May 28.

Institute for Medical Biology and Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan, China.

Placenta-specific protein 9 (PLAC9) is a putative secretory protein that was initially identified in the placenta and is involved in cell proliferation and motility. Bioinformatics analyses revealed that PLAC9 is repressed in lung cancers (LCs), especially lung adenocarcinomas, compared to that in the paired adjacent normal tissues, indicating that PLAC9 might be involved in the pathogenesis of pulmonary diseases. To investigate the potential role of PLAC9 in the abnormal reprogramming of airway epithelial cells (AECs), a key cause of pulmonary diseases, we constructed a stable PLAC9-overexpressing human bronchial epithelial cell line (16HBE-GFP-). We utilized the proteomic approach isobaric tag for relative and absolute quantification (iTRAQ) to analyze the effect of PLAC9 on cellular protein composition. Gene ontology (GO) and pathway analyses revealed that GO terms and pathways associated with cell proliferation, cell cycle progression, and cell motility and migration were significantly enriched among the proteins regulated by PLAC9. Our results showed that PLAC9 overexpression reduced cell proliferation, altered cell cycle progression, and increased cell motility, including migration and invasion. Our findings suggest that PLAC9 inhibits cell proliferation through S phase arrest by altering the expression levels of cyclin/cyclin-dependent kinases (CDKs) and promotes cell motility, likely the concerted actions of cyclins, E-cadherin, and vimentin. Since these mechanisms may underlie PLAC9-mediated abnormal human bronchial pathogenesis, our study provides a basis for the development of molecular targeted treatments for LCs.
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http://dx.doi.org/10.3389/fonc.2021.628480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194706PMC
May 2021

High throughput proteomic and metabolic profiling identified target correction of metabolic abnormalities as a novel therapeutic approach in head and neck paraganglioma.

Transl Oncol 2021 Aug 9;14(8):101146. Epub 2021 Jun 9.

Department of Otolaryngology Head & Neck Surgery, The Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, No. 639, Zhi-Zao-Ju Road, Shanghai 200011, China; Ear Institute, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China. Electronic address:

Head and neck paragangliomas (HNPGLs) are rare neoplasms that represent difficult treatment paradigms in neurotology. Germline mutations in genes encoding succinate dehydrogenase (SDH) are the cause of nearly all familial HNPGLs. However, the molecular mechanisms underlying tumorigenesis remain unclear. Mutational analysis identified 6 out of 14 HNPGLs harboring clinicopathologic SDH gene mutations. The SDHB gene was most frequently mutated in these patients, and western blot showed loss of SDHB protein in tumors with SDHB mutations. The paraganglioma cell line (PGL-626) was established from a sample that harbored a missense SDHB mutation (c.649C > T). Spectrometric analysis using tandem mass tags identified 151 proteins significantly differentially expressed in HNPGLs compared with normal nerves. Bioinformatics analyses confirmed the high level of enrichment of oxidative phosphorylation and metabolism pathways in HNPGLs. The mitochondrial complex subunits NDUFA2, NDUFA10, and NDUFA4, showed the most significantly increased expression and were localized predominantly in the cytoplasm of PGL-626 cells. The mitochondrial complex I inhibitor metformin exerted dose-dependent inhibitory effects on PGL-626 cells via cooperative down-regulation of NDUFA2, 4, and 10, with a significant decrease in the levels of reactive oxygen species and mitochondrial membrane potential. Further metabolomic analysis of PGL-626 cells showed that metabolites involved in central carbon metabolism in cancer and sphingolipid signaling pathways, pantothenate and CoA biosynthesis, and tryptophan and carbon metabolism were significantly altered after metformin treatment. Thus, this study provides insights into the molecular mechanisms underlying HNPGL tumorigenesis and identifies target correction of metabolic abnormalities as a novel therapeutic approach for this disease.
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http://dx.doi.org/10.1016/j.tranon.2021.101146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193622PMC
August 2021

Astrocyte-selective STAT3 knockdown rescues methamphetamine withdrawal-disrupted spatial memory in mice via restoring the astrocytic capacity of glutamate clearance in dCA1.

Glia 2021 Jun 10. Epub 2021 Jun 10.

Department of Human Anatomy and Histoembryology, Nanjing University of Chinese Medicine, Nanjing, China.

Methamphetamine (METH) is a common abused drug. METH-triggered glutamate (Glu) levels in dorsal CA1 (dCA1) could partially explain the etiology of METH-caused abnormal memory, but the synaptic mechanism remains unclear. Here, we found that METH withdrawal disrupted spatial memory in mice, accompanied by the increases in Glu levels and postsynaptic neuronal activities at dCA1 synapses. METH withdrawal weakened the capacity of Glu clearance in astrocytes, as indicated by increasing the A1-like astrocytes and phosphorylated signal transducer and activator of transcription 3 (p-STAT3), decreasing the Glu transporter 1(GLT-1, also known as EAAT2 or SLC1A2), Glu-aspartate-transporter (GLAST also known as EAAT1 or SLC1A3) and astrocytic glutamine synthase (GS), but failed to affect the presynaptic Glu release from dCA3 within dCA1. Moreover, we identified that in vitro A1-like astrocytes exhibited an increased STAT3 activation and the impaired capacity of Glu clearance. Most importantly, selective knockdown of astrocytic STAT3 in vivo in dCA1 restored the astrocytic capacity of Glu clearance, normalized Glu levels at dCA1 synapses, and finally rescued METH withdrawal-disrupted spatial memory in mice. Thus, astrocytic Glu clearance system, especially STAT3, serves as a novel target for future therapies against METH neurotoxicity.
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http://dx.doi.org/10.1002/glia.24046DOI Listing
June 2021

Anti-Psoriatic Effects of Middle Fragment of Chlamydial Plasmid-Encoded Protein pGP3 in an Imiquimod-Induced Psoriasis Mouse Model.

Med Sci Monit 2021 Jun 5;27:e929781. Epub 2021 Jun 5.

Department of Dermatology and Venereology, Tianjin Medical University General Hospital, Tianjin, China (mainland).

BACKGROUND Previously, we demonstrated that the chlamydial protein pGP3 forms a stable complex with LL-37 to neutralize its proinflammatory activity during the pathogenesis of psoriasis. The middle domain of pGP3 (pGP3M) is critical for the binding and neutralization of LL-37. Here, we further examined the mechanism underlying pGP3-mediated inhibition of psoriasis progression and evaluated the inhibitory effect of pGP3M on the development of psoriasis-like skin lesions in mice. MATERIAL AND METHODS Stock solutions of pGP3M and pGP3 (100 μg/mL) were prepared using sterile ultrapure water and intramuscularly injected into the left leg of the imiquimod (IMQ)-induced psoriasis mouse model. The severity of skin lesions was evaluated based on the psoriasis area and severity index score and ear skin thickness. The skin biopsy and blood samples were collected on the 8th day for histological analysis and inflammatory cytokines detection. RESULTS Erythema, scaling, and thickening were observed on the dorsal skin and the right ear skin of IMQ-treated mice. Treatment with pGP3 and pGP3M alleviated the IMQ-induced erythema, inflammatory cell infiltration, and scaly plaques. Compared with IMQ-treated and PBS-treated mice, pGP3- and PGP3M-treated mice had less inflammatory cell infiltration in skin tissues and had significantly reduced IL-17A, IFN-γ, and IL-22 levels in serum. CONCLUSIONS The anti-psoriatic efficacy of exogenous pGP3M was similar to that of pGP3. This indicated that pGP3M attenuated the IMQ-induced inflammatory and psoriatic symptoms in mice by binding and inhibiting LL-37. Further research is needed to examine the toxicity of pGP3 and pGP3M before clinical trial evaluation.
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http://dx.doi.org/10.12659/MSM.929781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188830PMC
June 2021

Comparing radiomics models with different inputs for accurate diagnosis of significant fibrosis in chronic liver disease.

Eur Radiol 2021 Apr 21. Epub 2021 Apr 21.

CAS Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, 95 Zhongguancun East Road, Beijing, 100190, China.

Objective: The non-invasive discrimination of significant fibrosis (≥ F2) in patients with chronic liver disease (CLD) is clinically critical but technically challenging. We aimed to develop an updated deep learning radiomics model of elastography (DLRE2.0) based on our previous DLRE model to achieve significantly improved performance in ≥ F2 evaluation.

Methods: This was a retrospective multicenter study with 807 CLD patients and 4842 images from three hospitals. All of these patients have liver biopsy results as referenced standard. Multichannel deep learning radiomics models were developed. Elastography images, gray-scale images of the liver capsule, gray-scale images of the liver parenchyma, and serological results were gradually integrated to establish different diagnosis models, and the optimal model was selected for assessing ≥ F2. Its accuracy was thoroughly investigated by applying different F0-1 prevalence cohorts and independent external test cohorts. Analysis of receiver operating characteristic (ROC) curves was performed to calculate the area under the ROC curve (AUC) for significance of fibrosis (≥ F2) and cirrhosis (F4).

Results: The AUC of the DLRE2.0 model significantly increased to 0.91 compared with the DLRE model (AUC 0.83) when evaluating ≥ F2 (p = 0.0167). However, it did not show statistically significant differences as integrating gray-scale images and serological data into the DLRE2.0 model. AUCs of DLRE and DLRE2.0 increased, when there was higher F0-1 prevalence. All radiomics models had good robustness in the independent external test cohort.

Conclusions: DLRE2.0 was the most suitable model for staging significant fibrosis while considering the balance of diagnostic accuracy and clinical practicability.

Key Points: • The non-invasive discrimination of significant fibrosis (≥ F2) in patients with chronic liver disease (CLD) is clinically critical but technically challenging. • We aimed to develop an updated deep learning radiomics model of elastography (DLRE2.0) based on our previous DLRE model to achieve significantly improved performance in ≥ F2 evaluation. • Our study based on 807 CLD patients and 4842 images with liver biopsy found that DLRE2.0 was the most suitable model for staging significant fibrosis while considering the balance of diagnostic accuracy and clinical practicability.
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http://dx.doi.org/10.1007/s00330-021-07934-6DOI Listing
April 2021

Reactive oxygen species-evoked endoplasmic reticulum stress mediates 1-nitropyrene-induced epithelial-mesenchymal transition and pulmonary fibrosis.

Environ Pollut 2021 Aug 13;283:117134. Epub 2021 Apr 13.

Department of Toxicology, Anhui Medical University, Hefei, 230032, China. Electronic address:

1-Nitropyrene (1-NP) is one component of atmospheric fine particles. Previous report revealed that acute 1-NP exposure induced respiratory inflammation. This study aimed to investigate whether chronic 1-NP exposure induces pulmonary fibrosis. Male C57BL6/J mice were intratracheally instilled to 1-NP (20 μg/mouse/week) for 6 weeks. Diffuse interstitial inflammation, a-smooth muscle actin (a-SMA)-positive cells, a marker of epithelial-mesenchymal transition (EMT), and an extensive collagen deposition, measured by Masson staining, were observed in 1-NP-exposed mouse lungs. Pulmonary function showed that lung dynamic compliance (Cydn-min) was reduced in 1-NP-exposed mice. Conversely, inspiratory resistance (Ri) and expiratory resistance (Re) were elevated in 1-NP-exposed mice. Mechanistically, cell migration and invasion were accelerated in 1-NP-exposed pulmonary epithelial cells. In addition, E-cadherin, an epithelial marker, was downregulated, and vimentin, a-SMA and N-cadherin, three mesenchymal markers, were upregulated in 1-NP-exposed pulmonary epithelial cells. Although TGF-β wasn't altered, phosphorylated Smad2/3 were enhanced in 1-NP-exposed pulmonary epithelial cells. Moreover, reactive oxygen species (ROS) were increased and endoplasmic reticulum (ER) stress was activated in 1-NP-exposed pulmonary epithelial cells. N-Acetylcysteine (NAC), an antioxidant, attenuated 1-NP-evoked excess ROS, ER stress and EMT in pulmonary epithelial cells. Similarly, pretreatment with NAC alleviated 1-NP-caused pulmonary EMT and lung fibrosis in mice. These results demonstrate that ROS-evoked ER stress contributes, at least partially, to 1-NP-induced EMT and pulmonary fibrosis.
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http://dx.doi.org/10.1016/j.envpol.2021.117134DOI Listing
August 2021

Nutrient deposition over the past 60 years in a reservoir within a medium-sized agricultural catchment.

Sci Total Environ 2021 Apr 14;764:142896. Epub 2020 Oct 14.

Shandong Provincial Key Laboratory of Water and Soil Conservation and Environmental Protection, Linyi University, Linyi, Shandong 276005, China.

Widespread dam construction has reduced the hydrological connectivity of catchments of various sizes. Nutrients lost from upper catchments can be intercepted by dams and deposited in reservoir sediments, and this reduces downstream nutrient transportation. Few previous studies have assessed historical nutrient deposition rates (NDRs) in man-made reservoirs. Using Cs and Pb deposition chronologies, this paper examines the total nitrogen (TN), total phosphate (TP), and organic matter (OM) concentrations in six sediment cores recovered from Xujiaya reservoir (catchment area 580 km), provides estimates of historical NDRs by referencing the original capacity curve, and explores temporal changes to the NDR since dam construction. The results show that anthropogenic sources resulted in the increase in nutrient concentrations in the upper parts of the cores, whereas natural sources were the main contributors to nutrient deposition across the whole reservoir. In addition, sediment supply from the catchment was the main source of the nutrients deposited in the reservoir, and the changing patterns of NDRs were overall regulated by sediment yields. The TN, TP, and OM profiles in the six cores, together with the historical NDRs, reflect the pattern of nutrient deposition in the reservoir derived from this agricultural catchment over the past 60 years. The results provide new insight into the effect of dam construction on nutrient deposition at a medium-sized catchment scale against a background of recent environmental change influenced by human activity.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142896DOI Listing
April 2021

Trimetallic Spinel NiCo Fe O Nanoboxes for Highly Efficient Electrocatalytic Oxygen Evolution.

Angew Chem Int Ed Engl 2021 May 15;60(21):11841-11846. Epub 2021 Apr 15.

School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Singapore.

The development of efficient and low-cost electrocatalysts toward the oxygen evolution reaction (OER) is critical for improving the efficiency of several electrochemical energy conversion and storage devices. Here, we report an elaborate design and synthesis of porous Co-based trimetallic spinel oxide nanoboxes (NiCo Fe O NBs) by a novel metal-organic framework engaged strategy, which involves chemical etching, cation exchange, and subsequent thermal oxidation processes. Owing to the structural and compositional advantages, the optimized trimetallic NiCo Fe O NBs (x is about 0.117) deliver superior electrocatalytic performance for OER with an overpotential of 274 mV at 10 mA cm , a small Tafel slope of 42 mV dec , and good stability in alkaline electrolyte, which is much better than that of Co-based bi/monometallic spinel oxides and even commercial RuO .
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http://dx.doi.org/10.1002/anie.202103058DOI Listing
May 2021

Dose-effect relationships of C ions-induced dicentric plus ring chromosomes, micronucleus and nucleoplasmic bridges in human lymphocytes in vitro.

Int J Radiat Biol 2021 25;97(5):657-663. Epub 2021 Mar 25.

China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing, P. R. China.

Purpose: The objective of this research was to explore the dose-effect relationships of dicentric plus ring (dic + r), micronucleus (MN) and nucleoplasmic bridges (NPB) induced by carbon ions in human lymphocytes.

Materials And Methods: Venous blood samples were collected from three healthy donors. C ions beam was used to irradiate the blood samples at the energy of 330 MeV and linear energy transfer (LET) of 50 keV/μm with a dose rate of 1 Gy/min in the spread-out Bragg peak. The irradiated doses were 0 (sham irradiation), 1, 2, 3, 4, 5 and 6 Gy. Dic + r chromosomes aberrations were scored in metaphases. The cytokinesis-block micronucleus cytome (CBMN) was conducted to analyze MN and NPB. The maximum low-dose relative biological effectiveness (RBE) values of the induction of dic + r, MN and NPB in human lymphocytes for C ions irradiation was calculated relative to Co γ-rays.

Results: The frequencies of dic + r, MN and NPB showed significantly increases in a dose-depended manner after exposure to C ions. The distributions of dic + r and MN exhibited overdispersion, while the distribution of NPB agreed with Poisson distribution at all doses. Linear-quadratic equations were established based on the frequencies of dic + r and MN. The dose-response curves of NPB frequencies followed a linear model. The derived RBE values for dic + r, MN and NPB in human lymphocytes irradiated with C ions were 8.07 ± 2.73, 2.69 ± 0.20 and 4.00 ± 2.69 in comparison with Co γ-rays.

Conclusion: The dose-response curves of carbon ions-induced dic + r, MN and NPB were constructed. These results could be helpful to improve radiation risk assessment and dose estimation after exposed to carbon ions irradiation.
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http://dx.doi.org/10.1080/09553002.2021.1900945DOI Listing
March 2021

Crystal structures of anthranilate phosphoribosyltransferase from Saccharomyces cerevisiae.

Acta Crystallogr F Struct Biol Commun 2021 Mar 3;77(Pt 3):61-69. Epub 2021 Mar 3.

School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.

Anthranilate phosphoribosyltransferase (AnPRT) catalyzes the transfer of the phosphoribosyl group of 5'-phosphoribosyl-1'-pyrophosphate (PRPP) to anthranilate to form phosphoribosyl-anthranilate. Crystal structures of AnPRTs from bacteria and archaea have previously been determined; however, the structure of Saccharomyces cerevisiae AnPRT (ScAnPRT) still remains unsolved. Here, crystal structures of ScAnPRT in the apo form as well as in complex with its substrate PRPP and the substrate analogue 4-fluoroanthranilate (4FA) are presented. These structures demonstrate that ScAnPRT exhibits the conserved structural fold of type III phosphoribosyltransferase enzymes and shares the similar mode of substrate binding found across the AnPRT protein family. In addition, crystal structures of ScAnPRT mutants (ScAnPRT and ScAnPRT) were also determined. These structures suggested that the conserved residue Ser121 is critical for binding PRPP, while Gly141 is dispensable for binding 4FA. In summary, these structures improved the preliminary understanding of the substrate-binding mode of ScAnPRT and laid foundations for future research.
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http://dx.doi.org/10.1107/S2053230X21001989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938636PMC
March 2021

Effects of radiation quality and dose rate on radiation-induced nucleoplasmic bridges in human peripheral blood lymphocytes.

Mutat Res 2021 Mar-Apr;863-864:503321. Epub 2021 Jan 28.

China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing, 100088, PR China. Electronic address:

Previous studies showed that the yield of cobalt-60 γ-rays-induced nucleoplasmic bridges (NPB) in human peripheral blood lymphocytes is dose dependent. However, the influence of the radiation quality and dose rates on NPB frequencies has not been investigated. The present study aimed to investigate NPB frequencies in human peripheral blood lymphocytes induced by carbon ions and explore the dose rate effect on cobalt-60 γ-rays-induced NPB. To establish dose-response curves, human peripheral blood samples were irradiated with 0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0 and 8.0 Gy of carbon ions at a dose rate of 3.0 Gy/min in vitro. To explore the dose rate effect, human peripheral blood samples were irradiated with 2.0 and 5.0 Gy of cobalt-60 γ-rays at dose rates of 0.2, 0.5, 1.0, 3.0, 5.0 and 10.0 Gy/min in vitro. NPB and micronuclei (MN) in binucleated cells were analyzed with the cytokinesis-block micronucleus cytome assay. Results showed that the dose-response curve of carbon ion-induced NPB frequencies follow a linear-quadratic model (R = 0.934). The relative biological effectiveness (RBE) values of carbon ions to cobalt-60 γ-rays decreased with increased NPB frequencies (ranging from 2.47 to 5.86). Compared with group 1.0 Gy/min, the NPB frequencies in groups 10.0 Gy/min (2.0 Gy), 5.0 and 10.0 Gy/min (5.0 Gy) were decreased significantly (P < 0.05). Carbon ion-induced NPB in human peripheral blood lymphocytes have a good dose-response relationship. Cobalt-60 γ-rays-induced NPB frequencies are affected by the specific dose rate.
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http://dx.doi.org/10.1016/j.mrgentox.2021.503321DOI Listing
April 2021

The Cancer-Testis Long Non-coding RNA PCAT6 Facilitates the Malignant Phenotype of Ovarian Cancer by Sponging miR-143-3p.

Front Cell Dev Biol 2021 4;9:593677. Epub 2021 Feb 4.

Nanjing Maternity and Child Health Care Hospital, Women's Hospital of Nanjing Medical University, Nanjing, China.

It has been reported that long non-coding RNAs (lncRNAs) play critical roles in tumorigenesis. However, their roles in ovarian cancer (OC) remain to be elucidated. The aim of this study was to uncover the function and underlying mechanisms of PCAT6 in OC. The expression pattern of PCAT6 in OC was analyzed in the GSE137238, GSE143897 and Gene Expression Profile Interactive Analysis (GEPIA) datasets. Kaplan-Meier Plotter online software was used for survival analysis. Loss-of-function assays and gain-of-function assays were used to assess the function of PCAT6 in OC development. Moreover, small-RNA sequencing, bioinformatic analysis, luciferase assays and rescue experiments were carried out to clarify the potential mechanism of PCAT6 in OC. PCAT6 expression was significantly increased in OC tissues and positively correlated with advanced stages and with poor overall survival, progression-free survival and post-progression survival. Knockdown of PCAT6 in A2780 and SKOV3 cells inhibited OC cell proliferation, migration and invasion. In contrast, Overexpression of PCAT6 exerted the opposite effects on OC cells. Notably, PCAT6 bound to miR-143-3p and affected the expression of transforming growth factor (TGF)-β-activated kinase 1 (TAK1). Subsequent rescue assays confirmed that upregulation of miR-143-3p decreased the PCAT6 overexpression-induced promotion of proliferation, migration and invasion. Moreover, downregulation of miR-143-3p reversed the PCAT6 knockdown-induced inhibition of proliferation, migration, and invasion. Our findings demonstrate that PCAT6 plays an oncogenic role in OC and may be useful as a therapeutic target for OC.
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http://dx.doi.org/10.3389/fcell.2021.593677DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902004PMC
February 2021

Metal-Organic Frameworks Derived Functional Materials for Electrochemical Energy Storage and Conversion: A Mini Review.

Nano Lett 2021 Feb 10;21(4):1555-1565. Epub 2021 Feb 10.

School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore 637459, Singapore.

With many apparent advantages including high surface area, tunable pore sizes and topologies, and diverse periodic organic-inorganic ingredients, metal-organic frameworks (MOFs) have been identified as versatile precursors or sacrificial templates for preparing functional materials as advanced electrodes or high-efficiency catalysts for electrochemical energy storage and conversion (EESC). In this Mini Review, we first briefly summarize the material design strategies to show the rich possibilities of the chemical compositions and physical structures of MOFs derivatives. We next highlight the latest advances focusing on the composition/structure/performance relationship and discuss their practical applications in various EESC systems, such as supercapacitors, rechargeable batteries, fuel cells, water electrolyzers, and carbon dioxide/nitrogen reduction reactions. Finally, we provide some of our own insights into the major challenges and prospective solutions of MOF-derived functional materials for EESC, hoping to shed some light on the future development of this highly exciting field.
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http://dx.doi.org/10.1021/acs.nanolett.0c04898DOI Listing
February 2021

Activity and bioavailability of food protein-derived angiotensin-I-converting enzyme-inhibitory peptides.

Compr Rev Food Sci Food Saf 2021 03 2;20(2):1150-1187. Epub 2021 Feb 2.

School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, Australia.

Angiotensin-I-converting enzyme (ACE) inhibitory peptides are able to inhibit the activity of ACE, which is the key enzymatic factor mediating systemic hypertension. ACE-inhibitory peptides can be obtained from edible proteins and have the function of antihypertension. The amino acid sequences and the secondary structures of ACE-inhibitory peptides determine the inhibitory activities and stability. The resistance of ACE-inhibitory peptides to digestive enzymes and peptidase affect their antihypertensive bioactivity in vivo. In this paper, the mechanism of ACE-inhibition, sources of the inhibitory peptides, structure-activity relationships, stability during digestion, absorption and transportation of ACE-inhibitory peptides, and consumption of ACE-inhibitory peptides are reviewed, which provide guidance to the development of new functional foods and production of antihypertensive nutraceuticals and pharmaceuticals.
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http://dx.doi.org/10.1111/1541-4337.12711DOI Listing
March 2021

Nitrogen-Doped Amorphous Zn-Carbon Multichannel Fibers for Stable Lithium Metal Anodes.

Angew Chem Int Ed Engl 2021 Apr 3;60(15):8515-8520. Epub 2021 Mar 3.

School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Singapore.

The application of lithium metal anodes for practical batteries is still impeded by safety issues and low Coulombic efficiency caused mainly by the uncontrollable growth of lithium dendrites. Herein, two types of free-standing nitrogen-doped amorphous Zn-carbon multichannel fibers are synthesized as multifunctional hosts for lithium accommodation. The 3D macroporous structures endow effectively reduced local current density, and the lithiophilic nitrogen-doped carbon and functional Zn nanoparticles serve as preferred deposition sites with low nucleation barriers to guide uniform lithium deposition. As a result, the developed anodes exhibit remarkable electrochemical properties in terms of high Coulombic efficiency for more than 500 cycles at various current densities from 1 to 5 mA cm , and symmetric cells show long-term cycling duration over 2000 h. Moreover, full cells based on the developed anode and a LiFePO cathode also demonstrate superior rate capability and stable cycle life.
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http://dx.doi.org/10.1002/anie.202100471DOI Listing
April 2021

Compound Heterozygous Mutations in the F7 Gene in 2 Unrelated Families With Congenital Factor VII Deficiency.

J Pediatr Hematol Oncol 2021 Jan 20. Epub 2021 Jan 20.

Department of Pediatric Hematology, The First Hospital of Jilin University, Changchun, China.

Factor VII (FVII) deficiency is a rare bleeding disorder normally caused by homozygous and compound heterozygous mutations in the F7 gene. Whole-exome sequencing was performed to identify F7 mutations in 3 individuals from 2 unrelated families who were diagnosed with FVII deficiency. Four compound heterozygous mutations were identified and validated in these 3 probands with FVII deficiency. Among the 4 identified mutations, NM_000131.4:c.572-1_581del, NM_000131.4:c.1250A>G (p.Tyr417Cys), and NM_000131.4:c.647G>T (p.Gly216Val) were novel. All 3 novel mutations were predicted to be likely pathogenic by the American College of Medical Genetics and Genomics/Association for Molecular Pathology guidelines.
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http://dx.doi.org/10.1097/MPH.0000000000002057DOI Listing
January 2021

Two-dimensional shear wave elastography predicts survival in advanced chronic liver disease.

Gut 2021 Jan 21. Epub 2021 Jan 21.

Department of Radiology, Beaujon University Hospital, Clichy, France.

Objective: Liver stiffness measurement (LSM) is a tool used to screen for significant fibrosis and portal hypertension. The aim of this retrospective multicentre study was to develop an easy tool using LSM for clinical outcomes in advanced chronic liver disease (ACLD) patients.

Design: This international multicentre cohort study included a derivation ACLD patient cohort with valid two-dimensional shear wave elastography (2D-SWE) results. Clinical and laboratory parameters at baseline and during follow-up were recorded. LSM by transient elastography (TE) was also recorded if available. The primary outcome was overall mortality. The secondary outcome was the development of first/further decompensation.

Results: After screening 2148 patients (16 centres), 1827 patients (55 years, 62.4% men) were included in the 2D-SWE cohort, with median liver SWE (L-SWE) 11.8 kPa and a model for end stage liver disease (MELD) score of 8. Combination of MELD score and L-SWE predict independently of mortality (AUC 0.8). L-SWE cut-off at ≥20 kPa combined with MELD ≥10 could stratify the risk of mortality and first/further decompensation in ACLD patients. The 2-year mortality and decompensation rates were 36.9% and 61.8%, respectively, in the 305 (18.3%) high-risk patients (with L-SWE ≥20 kPa and MELD ≥10), while in the 944 (56.6%) low-risk patients, these were 1.1% and 3.5%, respectively. Importantly, this M10LS20 algorithm was validated by TE-based LSM and in an additional cohort of 119 patients with valid point shear SWE-LSM.

Conclusion: The M10LS20 algorithm allows risk stratification of patients with ACLD. Patients with L-SWE ≥20 kPa and MELD ≥10 should be followed closely and receive intensified care, while patients with low risk may be managed at longer intervals.
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http://dx.doi.org/10.1136/gutjnl-2020-323419DOI Listing
January 2021

Predictive value of red blood cell distribution width in critically ill patients with atrial fibrillation: a retrospective cohort study.

Ann Palliat Med 2021 Mar 18;10(3):2469-2480. Epub 2021 Jan 18.

Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China; Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, Hangzhou, China.

Background: Atrial fibrillation (AF) is a leading cause of morbidity and mortality among elderly patients especially for patients in ICU. Previous studies revealed the impact of red blood cell distribution width (RDW) on predicting onset of AF. However, the prognostic value of RDW in critically ill patients with AF remains largely unknown. Thus, this study aims to explore the potential value on predicting in- and out-of-hospital mortality in critically ill patients with AF.

Methods: Data were extracted from the Medical Information Mart for Intensive Care (MIMIC) III database and 7,867 critically ill patients with AF were enrolled. The association between RDW and inhospital mortality was evaluated using the multiple logistic regression analysis as a design variable. Shortand long-term outcomes were compared between the low RDW and high RDW groups in critically ill patients balanced by the propensity score matching (PSM) algorithm.

Results: Analysis of the 7,867 patients revealed a linear relationship between RDW and in-hospital mortality. For critically ill patients with AF, the elevated level of RDW was associated with increased inhospital mortality, with the OR increasing from level 2 (OR: 1.75, 95% CI: 1.25 to 2.44) to level 5 (OR: 3.89, 95% CI: 2.55 to 5.93) with level 1 (RDW ≤13) as the reference group. 3841 enrolled patients with records in the CareVue systems were selected by PSM algorithm. The baseline characters were well balanced in 1,054 pairs of enrolled patients. A significant lower survival rate was observed in the high RDW group (P<0.001).

Conclusions: High levels of RDW are associated with increased in- and out-of-hospital mortality in critically ill patients with AF.
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http://dx.doi.org/10.21037/apm-20-1704DOI Listing
March 2021

A finite element analysis of relationship between fracture, implant and tibial tunnel.

Sci Rep 2021 Jan 19;11(1):1781. Epub 2021 Jan 19.

Department of Nuclear Medicine, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, China.

The purpose of this article was to use finite element analysis (FEA) to study the relationship of tibial tunnel (TT) with fracture pattern and implants. A computed tomography scan of full-length tibia and fibula was obtained. Models were built after three-dimensional reconstruction. The corresponding plates and screws were constructed and assembled together with fracture models. FEA was performed and contourplots were output. The Von Mises stresses of nodes and displacements of elements were extracted. Student's t test was used to compare the values of Von Mises stresses and displacements between corresponding models. Differences in Von Mises stresses and displacements of fragments and implants between models with and without TT were nearly all statistically significant. However, the displacements of fragments and implants for all models were < 2 mm. TT in fracture models had larger Von Mises stresses than TT in intact tibial model. However, displacements of TT in fracture models showed similar or even smaller results to those in intact tibial model. Although almost all the tested parameters were statistically significant, differences were small and values were all below the clinical threshold. This study could promote open reduction and internal fixation with one-stage reconstruction for treatment of tibial plateau fractures associated with anterior cruciate ligament (ACL) ruptures.
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http://dx.doi.org/10.1038/s41598-021-81401-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815705PMC
January 2021

Multipolar Plasmonic Resonances of Aluminum Nanoantenna Tuned by Graphene.

Nanomaterials (Basel) 2021 Jan 13;11(1). Epub 2021 Jan 13.

College of Science, Zhejiang University of Technology, Hangzhou 310023, China.

We numerically investigate the multipolar plasmonic resonances of Aluminum nanoantenna tuned by a monolayer graphene from ultraviolet (UV) to visible regime. It is shown that the absorbance of the plasmonic odd modes ( = 1 and = 3) of graphene-Al nanoribbon structure is enhanced while the absorption at the plasmonic even modes ( = 2) is suppressed, compared to the pure Al nanoribbon structure. With the presence of the monolayer graphene, a change in the resonance strength of the multipolar plasmonic modes results from the near field interactions of the monolayer graphene with the electric fields of the multipolar plasmonic resonances of the Al resonator. In particular, a clear absorption peak with a high quality ()-factor of 27 of the plasmonic third-order mode ( = 3) is realized in the graphene-Al nanoribbon structure. The sensitivity and figure of merit of the plasmonic third-order mode of the proposed Graphene-Al nanoribbon structure can reach 25 nm/RIU and 3, respectively, providing potential applications in optical refractive-index sensing.
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http://dx.doi.org/10.3390/nano11010185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828546PMC
January 2021

Identification of Potential Radiation Responsive Metabolic Biomarkers in Plasma of Rats Exposed to Different Doses of Cobalt-60 Gamma Rays.

Dose Response 2020 Oct-Dec;18(4):1559325820979570. Epub 2020 Dec 15.

China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

Metabolomics has great potential to process accessible biofluids through high-throughput and quantitative analysis for radiation biomarker screening. This study focused on the potential radiation responsive metabolites in rat plasma and the dose-response relationships. In the discovery stage, 20 male Sprague-Dawley rats were exposed to 0, 1, 3 and 5 Gy of cobalt-60 gamma rays at a dose rate of 1 Gy/min. Plasma samples were collected at 72 h after exposure and analyzed using liquid chromatography mass spectrometry based on non-targeted metabolomics. In the verification stage, 50 additional rats were exposed to 0, 1, 2, 3, 5 and 8 Gy of gamma rays. The concentrations of candidate metabolites were then analyzed using targeted metabolomics methods. Fifteen candidate radiation responsive metabolites were identified as potential radiation metabolite biomarkers. Metabolic pathways, such as linoleic acid metabolism and glycerophospholipid metabolism pathways, were changed after irradiation. Six radiation responsive metabolites, including LysoPC(20:2), LysoPC(20:3), PC(18:0/22:5), L-palmitoylcarnitine, N-acetylornithine and butyrylcarnitine, had good dose-response relationships ( > 0.80). The area under the curve of the panel of the 6 radiation responsive metabolites was 0.923. The radiation exposure metabolomics biomarkers and dose-response curves may have potential for rapid dose assessment and triage in nuclear and radiation accidents.
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http://dx.doi.org/10.1177/1559325820979570DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7745571PMC
December 2020

sp. nov. and sp. nov. (Cryptococcaceae, Tremellales), two novel basidiomycetous yeast species isolated from flowers.

Int J Syst Evol Microbiol 2020 Oct;70(10):5394-5400

State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Science, Beijing 100101, PR China.

Three yeast strains isolated from three flower samples were identified as representing two novel species of based on molecular phylogenetic analysis and phenotypic comparisons. Strains 12A8 and 21S4 with pink cream colonies and subglobose to globose cells had identical sequences in the ITS and LSU D1/D2 regions, which differed from strain X54 with cream colonies and ovoid to ellipsoidal cells by 6 nt substitutions (1 %) and 9 nt mismatches (1.5 %) in the D1/D2 domains and ITS region, respectively. They could also be distinguished from each other in assimilation of glucitol and salicin, growth at 28 °C and cell fibrillar appendages under scanning electron microscopy. The three strains differed from known species of by more than 8 nt (1.3 %) and 30 nt (5 %) in the D1/D2 domains and ITS region, respectively. Therefore, the names sp. nov. (Holotype CGMCC 2.5840, Mycobank MB 835892) and sp. nov. (Holotype CGMCC 2.5830, MycoBank MB 835891) are proposed to accommodate strain X54, and strains 12A8 and 21S4, respectively.
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http://dx.doi.org/10.1099/ijsem.0.004423DOI Listing
October 2020
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