Publications by authors named "Lu Wei"

2,670 Publications

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A multi-center survey on the postpartum mental health of mothers and attachment to their neonates during COVID-19 in Hubei Province of China.

Ann Transl Med 2021 Mar;9(5):382

Health, Nutrition and WASH, UNICEF China, Beijing, China.

Background: There is an emerging literature on the mental health of both pre- and post-partum mothers during the coronavirus disease 2019 (COVID-19) pandemic.

Methods: As of April 1, 2020, 23 mothers confirmed with COVID-19, 15 mothers suspected with COVID-19 but with negative polymerase chain reaction tests, and 33 mothers without COVID-19 (Control Group) were recruited for a study from Hubei Province in China. The Maternal Postnatal Attachment Scale (MPAS), the Zung Self-rating Anxiety Scale, and the Zung Self-rating Depression Scale were applied to investigate the attachment of mothers to their neonates and the postpartum mental health of mothers within the first 3 months after delivery (between 20 to 89 days).

Results: The period of mother-child separation among the confirmed group (33.9±20.9 days) was significantly longer than that of suspected group (16.7±12.2 days) and control group (10.7±8.4 days). The total score of the MPAS in mothers confirmed with COVID-19 (45.5±4.2) was significantly lower (indicating less mother-child attachment) than that in the suspected (50.5±4.7) and control (48.8±4.6) groups. A negative correlation was noted between the mother-child separation time and the MPAS scores, including the subscale scores of attachment (MPAS acore: Spearman's ρ =-0.33, 95% CI: -0.095 to -0.538, P=0.005; Subscale score of attachment: Spearman's ρ =-0.40, 95% CI: -0.163 to -0.592, P=0.001). The incidence of postpartum anxiety in the confirmed, suspected and control groups was 4.3%, 6.7% and 12.1%, respectively; and the incidence of postpartum depression was 39.1%, 33.3% and 30.3%, respectively. No significant difference was found with regards to maternal postpartum anxiety and depression among the three groups.

Conclusions: Decreased mother-child attachment found among mothers confirmed with COVID-19, indicates that further intervention is needed to ensure mother-child interaction to appropriately develop attachment. Mother-child attachment experienced disruption due to prolonged mother-child separation necessitated by the COVID-19 management protocol, which needs to be revised to reduce prolonged mother-child separation. Additionally, mothers with and without COVID-19 suffered a high incidence of depression, which warrants further mental health investment for pregnant mothers during the COVID-19 pandemic.
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http://dx.doi.org/10.21037/atm-20-6115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033344PMC
March 2021

Toward photoswitchable electronic pre-resonance stimulated Raman probes.

J Chem Phys 2021 Apr;154(13):135102

Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.

Reversibly photoswitchable probes allow for a wide variety of optical imaging applications. In particular, photoswitchable fluorescent probes have significantly facilitated the development of super-resolution microscopy. Recently, stimulated Raman scattering (SRS) imaging, a sensitive and chemical-specific optical microscopy, has proven to be a powerful live-cell imaging strategy. Driven by the advances of newly developed Raman probes, in particular the pre-resonance enhanced narrow-band vibrational probes, electronic pre-resonance SRS (epr-SRS) has achieved super-multiplex imaging with sensitivity down to 250 nM and multiplexity up to 24 colors. However, despite the high demand, photoswitchable Raman probes have yet to be developed. Here, we propose a general strategy for devising photoswitchable epr-SRS probes. Toward this goal, we exploit the molecular electronic and vibrational coupling, in which we switch the electronic states of the molecules to four different states to turn their ground-state epr-SRS signals on and off. First, we showed that inducing transitions to both the electronic excited state and triplet state can effectively diminish the SRS peaks. Second, we revealed that the epr-SRS signals can be effectively switched off in red-absorbing organic molecules through light-facilitated transitions to a reduced state. Third, we identified that photoswitchable proteins with near-infrared photoswitchable absorbance, whose states are modulable with their electronic resonances detunable toward and away from the pump photon energy, can function as the photoswitchable epr-SRS probes with desirable sensitivity (<1 µM) and low photofatigue (>40 cycles). These photophysical characterizations and proof-of-concept demonstrations should advance the development of novel photoswitchable Raman probes and open up the unexplored Raman imaging capabilities.
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http://dx.doi.org/10.1063/5.0043791DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019356PMC
April 2021

ELAV/Hu RNA binding proteins determine multiple programs of neural alternative splicing.

PLoS Genet 2021 Apr 7;17(4):e1009439. Epub 2021 Apr 7.

Developmental Biology Program, Sloan Kettering Institute, New York City, New York, United States of America.

ELAV/Hu factors are conserved RNA binding proteins (RBPs) that play diverse roles in mRNA processing and regulation. The founding member, Drosophila Elav, was recognized as a vital neural factor 35 years ago. Nevertheless, little was known about its impacts on the transcriptome, and potential functional overlap with its paralogs. Building on our recent findings that neural-specific lengthened 3' UTR isoforms are co-determined by ELAV/Hu factors, we address their impacts on splicing. While only a few splicing targets of Drosophila are known, ectopic expression of each of the three family members (Elav, Fne and Rbp9) alters hundreds of cassette exon and alternative last exon (ALE) splicing choices. Reciprocally, double mutants of elav/fne, but not elav alone, exhibit opposite effects on both classes of regulated mRNA processing events in larval CNS. While manipulation of Drosophila ELAV/Hu RBPs induces both exon skipping and inclusion, characteristic ELAV/Hu motifs are enriched only within introns flanking exons that are suppressed by ELAV/Hu factors. Moreover, the roles of ELAV/Hu factors in global promotion of distal ALE splicing are mechanistically linked to terminal 3' UTR extensions in neurons, since both processes involve bypass of proximal polyadenylation signals linked to ELAV/Hu motifs downstream of cleavage sites. We corroborate the direct action of Elav in diverse modes of mRNA processing using RRM-dependent Elav-CLIP data from S2 cells. Finally, we provide evidence for conservation in mammalian neurons, which undergo broad programs of distal ALE and APA lengthening, linked to ELAV/Hu motifs downstream of regulated polyadenylation sites. Overall, ELAV/Hu RBPs orchestrate multiple broad programs of neuronal mRNA processing and isoform diversification in Drosophila and mammalian neurons.
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http://dx.doi.org/10.1371/journal.pgen.1009439DOI Listing
April 2021

Effects of nitrogen application rate on the photosynthetic pigment, leaf fluorescence characteristics, and yield of indica hybrid rice and their interrelations.

Sci Rep 2021 Apr 5;11(1):7485. Epub 2021 Apr 5.

College of Agronomy, Guizhou University, Guiyang, 550025, China.

A field experiment employing the rice cultivars Qyou6 and Yixiangyou2115 as materials and different nitrogen application rates was conducted in Huangping County, Guizhou Province in 2019 to determine the effects of nitrogen application rate on photosynthetic pigments, leaf fluorescence characteristics, yield, and their interrelations in indica hybrid rice. The results showed that photosynthetic pigment contents generally increased with increasing nitrogen application rate. As the nitrogen rate increased, the maximal quantum yield of PSII (F/F), actual quantum yield of PSII (Φ), and relative electron transfer rate at PSII (ETR) first decreased and then increased at the booting stage; F/F and Φ decreased while ETR first increased and then decreased at the heading stage; nevertheless, F/F and Φ first decreased and then increased but ETR was just the opposite at the maturity stage. Non-photochemical quenching coefficient (qN) and quantum yield of regulatory energy dissipation at PSII (Y(NPQ)) first increased and then decreased whereas quantum yield of non-regulatory energy dissipation at PSII (Y(NO)) first decreased and then increased at the booting, heading, and maturity stages with increasing nitrogen application rate. Photochemical quenching coefficient (qP) showed an increasing trend as the nitrogen rate increased in the range of 150-300 kg/ha at the heading and maturity stages. Photosynthetic pigments, leaf fluorescence characteristics, and yield and its components were significantly correlated. First, chlorophylls a and b were significantly negatively correlated with F/F while significantly positively correlated with qP at the heading stage. Secondly, Carotenoids were significantly positively correlated with the effective panicle number (EPN) at the booting stage while significantly negatively correlated with the spikelets per panicle (SPP) at the heading stage. Chlorophyll a and carotenoids were significantly positively correlated with EPN but significantly negatively correlated with spikelet filling (SF) at the maturity stage. In addition, qP was significantly negatively correlated with EPN at the booting stage. At the heading stage, F/F and Y(NO) were significantly negatively correlated with EPN and SPP, respectively, and F/F and Φ were significantly positively related to SF. Moreover, qP was extremely significantly positively related to EPN whereas F/F was extremely significantly negatively correlated with grain yield at the maturity stage. Appropriate nitrogen application rates can enhance photosynthetic pigment contents, improve the photochemical efficiency and proportion of the open part of the reaction center of PSII, and promote the quantum efficiency and self-protection ability of PSII, thereby increasing photosynthetic efficiency and yield. Under the conditions adopted in this experiment, a parabolic relationship was observed between the nitrogen application rate and grain yield. The regression analysis results showed that the best nitrogen application rate of indica hybrid rice is 168.16 kg ha and the highest yield is 11,804.87 kg ha.
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http://dx.doi.org/10.1038/s41598-021-86858-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021548PMC
April 2021

Combined role of polarization matching and critical coupling in enhanced absorption of 2D materials based on metamaterials.

Opt Express 2021 Mar;29(6):9269-9282

Since 2D materials are typically much more efficient to absorb in-plane polarized light than out-of-plane polarized light, keeping the light polarization in-plane at the 2D material is revealed to be a crucial factor other than critical coupling in light absorption enhancement in a 2D material integrated with a light coupling structure. When the composite of a metal-insulator-metal structure and a 2D material changes from the magnetic resonator form to the metasurface Salisbury screen one, the field polarization at the 2D material changes from a mainly out-of-plane status to a mainly in-plane status. As a result, for graphene, the absorptance enhancement is increased by 1.6 to 4.2 times, the bandwidth enlarged by 3.6 to 6.4 times, and the metal loss suppressed by 7.4 to 24 times in the mid- to far-infrared range, leading to the absorptance of graphene approaching 90% in the mid-infrared regime and 100% in the THz regime. For monolayer black phosphorus, the absorptance enhancement at the wavelength of 3.5 µm is increased by 5.4 times, and the bandwidth enlarged by 1.8 times. For monolayer MoS, the averaged absorptance in the visible-near infrared range is enhanced by 4.4 times from 15.5% to 68.1%.
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http://dx.doi.org/10.1364/OE.419028DOI Listing
March 2021

Relapse or Re-Infection, the Situation of Recurrent Tuberculosis in Eastern China.

Front Cell Infect Microbiol 2021 17;11:638990. Epub 2021 Mar 17.

Department of Chronic Communicable Disease, Center for Disease Control and Prevention of Jiangsu Province, Nanjing, China.

Purpose: Recurrent tuberculosis (TB) is defined by more than one TB episode per patient and is caused by re-infection with a new (Mtb) strain or relapse with the previous strain. Recurrence of TB is one important obstacle for End TB strategy in the world and elucidating the triggers of recurrence is important for the current TB control strategy in China. This study aimed to analyze the sources of recurrent TB by the molecular genotyping method.

Method: A population-based surveillance was undertaking on all culture-positive TB cases in Jiangsu province, China from 2013 to 2019. Phenotypic drug susceptibility test (DST) by proportion method and mycobacterial interspersed repetitive units-variable number of tandem repeat (MIRU-VNTR) were adopted for drug resistance and genotype detection.

Results: A total of 1451 culture-positive TB patients were collected and 30 (2.06%, 30/1451) TB cases had recurrent TB episodes. Except 7 isolates were failed during subculture, 23 paired isolates were assessed. After genotyping by MIRU-VNTR, 12 (52.17%, 12/23) paired recurrence TB were demonstrated as relapse and 11 (47.83%,11/23) paired cases were identified as re-infection. The average interval time for recurrence was 24.04 (95%CI: 19.37-28.71) months, and there was no significant difference between relapse and re-infection. For the relapsed cases, two paired isolates exhibited drug resistance shifting, while four paired isolates revealed inconsistent drug resistance among the re-infection group including two multidrug-resistant tuberculosis (MDR-TB) at the second episode.

Conclusion: Relapse and re-infection contributed equally to the current situation of recurrence TB in Jiangsu, China. Besides, more efficient treatment assessment, specific and vigorous interventions are urgently needed for MDR-TB patients, considering obvious performance among re-infection cases.
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http://dx.doi.org/10.3389/fcimb.2021.638990DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010194PMC
March 2021

Targeting ACLY Attenuates Tumor Growth and Acquired Cisplatin Resistance in Ovarian Cancer by Inhibiting the PI3K-AKT Pathway and Activating the AMPK-ROS Pathway.

Front Oncol 2021 17;11:642229. Epub 2021 Mar 17.

Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, China.

Ovarian cancer is the most lethal female genital malignancy. Although cisplatin is the first-line chemotherapy to treat ovarian cancer patients along with debulking surgeries, its efficacy is limited due to the high incidence of cisplatin resistance. ATP citrate lyase (ACLY) has been shown to be a key metabolic enzyme and is associated with poor prognosis in various cancers, including ovarian cancer. Nevertheless, no studies have probed the mechanistic relationship between ACLY and cisplatin resistance. Survival analysis was mainly carried out online. Bioinformatic analysis was performed in R/R studio. Proliferative activity was measured by MTT and colony formation assays. Cell cycle and apoptosis analysis were performed by flow cytometry. The acquired-cisplatin-resistant cell line A2780/CDDP was generated by exposing A2780 to cisplatin at gradually elevated concentrations. MTT assay was used to calculate IC values of cisplatin. A xenograft tumor assay was used test cell proliferation . Higher expression of ACLY was found in ovarian cancer tissue and related to poor prognosis. Knockdown of ACLY in A2780, SKOV3, and HEY cells inhibited cell proliferation, caused cell-cycle arrest by modulating the P16-CDK4-CCND1 pathway, and induced apoptosis probably by inhibiting p-AKT activity. Bioinformatic analysis of the GSE15709 dataset revealed upregulation of ACLY and activation of PI3K-AKT pathway in cells with acquired cisplatin resistance, in line with observations on A2780/CDDP cells that we generated. Knockdown of ACLY alleviated cisplatin resistance, and works synergistically with cisplatin treatment to induce apoptosis in A2780/CDDP cells by inhibiting the PI3K-AKT pathway and activating AMPK-ROS pathway. The ACLY-specific inhibitor SB-204990 showed the same effect. In A2780/CDDP cells, AKT overexpression could attenuate cisplatin re-sensitization caused by ACLY knockdown. Knockdown of ACLY attenuated cisplatin resistance by inhibiting the PI3K-AKT pathway and activating the AMPK-ROS pathway. These findings suggest that a combination of ACLY inhibition and cisplatin might be an effective strategy for overcoming cisplatin resistance in ovarian cancer.
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http://dx.doi.org/10.3389/fonc.2021.642229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011496PMC
March 2021

MK801 regulates the expression of key osteoarthritis factors in osteoarthritis synovial fibroblasts through complement C5.

Res Vet Sci 2021 Mar 3;136:377-384. Epub 2021 Mar 3.

Department of Anesthesiology, Guizhou Medical University, Guiyang 550002, PR China. Electronic address:

Background: Osteoarthritis is currently one of the most common chronic diseases. As life expectancy increases, its prevalence and incidence are expected to rise. At present, more and more evidences prove the correlation between the complement system and osteoarthritis (OA). This study aims to investigate complement C5's influence on the effect of MK801 on osteoarthritis synovial fibroblasts (OA-SFs).

Methods: We used IL-1b to induce OA-SFs derived from mice to obtain OA-SFs. And we performed RT-PCR and Western Blot assays to evaluate the expression levels of associated mRNA and protein. The alteration of MAC expression on OA-SFs cell membrane was evaluated by immunofluorescence assay. The expression of related inflammatory factors of OA-SFs was evaluated by ELISA experiment.

Results: MK801 could significantly inhibit the expression of osteoarthritis (OA) marker factors, such as: membrane attack complex (MAC), tumor necrosis factor-α (TNF-α) and matrix metalloproteinase-13 (MMP13). Meanwhile, MK801 can significantly inhibit the expression of complement C5 (C5) in OA-SFs. Immunofluorescence assay showed that MAC expression on OA-SFs cell membrane was significantly inhibited by MK801. The nucleo-plasmic separation experiment demonstrated that MK801 could significantly inhibit the activation of Nuclear factor-κB (NF-κB) signaling pathway in OA-SFs. Futhermore, koncking down the expression of C5 reversed the inhibition MK801 on the expression of OA-SFs inflammatory factors.

Conclusions: These results illustrated two points: first, MK801 inhibited the generation of MAC and the release of inflammation factors in OA-SFs through C5; second: MK801 inhibited the activation of NF-κB signaling pathway in OA-SFs.
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http://dx.doi.org/10.1016/j.rvsc.2021.03.003DOI Listing
March 2021

2D materials for conducting holes from grain boundaries in perovskite solar cells.

Light Sci Appl 2021 Mar 31;10(1):68. Epub 2021 Mar 31.

Department of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.

Grain boundaries in organic-inorganic halide perovskite solar cells (PSCs) have been found to be detrimental to the photovoltaic performance of devices. Here, we develop a unique approach to overcome this problem by modifying the edges of perovskite grain boundaries with flakes of high-mobility two-dimensional (2D) materials via a convenient solution process. A synergistic effect between the 2D flakes and perovskite grain boundaries is observed for the first time, which can significantly enhance the performance of PSCs. We find that the 2D flakes can conduct holes from the grain boundaries to the hole transport layers in PSCs, thereby making hole channels in the grain boundaries of the devices. Hence, 2D flakes with high carrier mobilities and short distances to grain boundaries can induce a more pronounced performance enhancement of the devices. This work presents a cost-effective strategy for improving the performance of PSCs by using high-mobility 2D materials.
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http://dx.doi.org/10.1038/s41377-021-00515-8DOI Listing
March 2021

Ultraviolet-Cured Semi-Interpenetrating Network Polymer Electrolytes for High-Performance Quasi-Solid-State Lithium Metal Batteries.

Chemistry 2021 Mar 29. Epub 2021 Mar 29.

Huazhong University of Science and Technology, School of Materials Science and Engineering, CHINA.

Solid polymer electrolytes with relatively low ionic conductivity at room temperature and poor mechanical strength greatly restrict their practical applications. Herein, we design semi-interpenetrating network polymer (SNP) electrolyte composed of an ultraviolet-crosslinked polymer network (ethoxylated trimethylolpropane triacrylate), linear polymer chains (polyvinylidene fluoride-co-hexafluoropropylene) and lithium salt solution to satisfy the demand of high ionic conductivity, good mechanical flexibility and electrochemical stability for lithium metal batteries. The semi-interpenetrating network has pivotal effect in improving chain relaxation, facilitating the local segmental motion of polymer chains and reducing the polymer crystallinity. Thanks to these advantages, the SNP electrolyte shows a high ionic conductivity (1.12 mS cm-1 at 30°C), wide electrochemical stability window (4.6 V vs. Li+/Li), good bendability and shape versatility. The promoted ion transport combined with suppressed impedance growth during cycling contribute to gratifying cell performances. The assembled quasi-solid-state lithium metal batteries (LiFePO4/SNP/Li) exhibit good cycling stability and rate capability at room temperature.
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http://dx.doi.org/10.1002/chem.202100380DOI Listing
March 2021

Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes and PEDF secretion.

Hum Cell 2021 Mar 25. Epub 2021 Mar 25.

Department of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, No 1 Jianshe Road, Zhengzhou, 450052, China.

Perivascular-resident macrophage-like melanocytes (PVM/Ms) can upregulate the expression of tight junction-related proteins in endothelial cells (ECs) by secreting pigment epithelial-derived factor (PEDF), and thereby regulate the permeability of the intrastrial fluid-blood barrier critical for maintaining inner ear homeostasis. This study aimed to investigate the effects of long non-coding RNA (lncRNA) Rian on cell growth of PVM/Ms and PVM/Ms regulation of intrastrial fluid-blood barrier integrity mediated by PEDF. Rian was downregulated in the aged cochlea from 12-month-old C57BL/6 mice. Rian overexpression inhibited cell apoptosis and promoted cell viability of hypoxia-injured PVM/Ms as well as increased the concentration and expression of PEDF secreted by PVM/Ms. In contrast, Rian silencing exerted the opposite effects. Furthermore, in a cell co-culture model of ECs and PVM/Ms, Rian overexpression in PVM/Ms increased the expression of the junction-associated proteins in co-cultured ECs, and this effect was abrogated by blockade of PEDF by anti-PEDF in PVM/Ms. Further mechanistical investigation revealed that Rian promoted STAT3 nuclear translocation and activation by binding to FUS, and thereby promoted the secretion of PEDF. Collectively, Rian attenuates PVM/Ms injury and strengthens the ability of PVM/Ms to maintain the integrity of the endothelial barrier by promoting PEDF expression.
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http://dx.doi.org/10.1007/s13577-021-00521-3DOI Listing
March 2021

Activity- and sleep-dependent regulation of tonic inhibition by Shisa7.

Cell Rep 2021 Mar;34(12):108899

Synapse and Neural Circuit Research Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address:

Tonic inhibition mediated by extrasynaptic γ-aminobutyric acid type A receptors (GABARs) critically regulates neuronal excitability and brain function. However, the mechanisms regulating tonic inhibition remain poorly understood. Here, we report that Shisa7 is critical for tonic inhibition regulation in hippocampal neurons. In juvenile Shisa7 knockout (KO) mice, α5-GABAR-mediated tonic currents are significantly reduced. Mechanistically, Shisa7 is crucial for α5-GABAR exocytosis. Additionally, Shisa7 regulation of tonic inhibition requires protein kinase A (PKA) that phosphorylates Shisa7 serine 405 (S405). Importantly, tonic inhibition undergoes activity-dependent regulation, and Shisa7 is required for homeostatic potentiation of tonic inhibition. Interestingly, in young adult Shisa7 KOs, basal tonic inhibition in hippocampal neurons is unaltered, largely due to the diminished α5-GABAR component of tonic inhibition. However, at this stage, tonic inhibition oscillates during the daily sleep/wake cycle, a process requiring Shisa7. Together, these data demonstrate that intricate signaling mechanisms regulate tonic inhibition at different developmental stages and reveal a molecular link between sleep and tonic inhibition.
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http://dx.doi.org/10.1016/j.celrep.2021.108899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025742PMC
March 2021

A machine learning-based survival prediction model of high grade glioma by integration of clinical and dose-volume histogram parameters.

Cancer Med 2021 Apr 24;10(8):2774-2786. Epub 2021 Mar 24.

Department of Radiation Oncology, Key Laboratory of Cancer Prevention and Intervention, Ministry of Education, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Purpose: Glioma is the most common type of primary brain tumor in adults, and it causes significant morbidity and mortality, especially in high-grade glioma (HGG) patients. The accurate prognostic prediction of HGG is vital and helpful for clinicians when developing therapeutic strategies. Therefore, we propose a machine learning-based survival prediction model by analyzing clinical and dose-volume histogram (DVH) parameters, to improve the performance of the risk model in HGG patients.

Methods: Eight clinical variables and 39 DVH parameters were extracted for each patient, who received radiotherapy for HGG with active follow-up. Ninety-five patients were randomly divided into training and testing cohorts, and we employed random survival forest (RSF), support vector machine (SVM), and Cox proportional hazards (CPHs) models to predict survival. Calibration plots, concordance indexes, and decision curve analyses were used to evaluate the calibration, discrimination, and clinical utility of these three models.

Results: The RSF model showed the best performance among the three models, with concordance indexes of 0.824 and 0.847 in the training and testing sets, respectively, followed by the SVM (0.792/0.823) and CPH (0.821/0.811) models. Specifically, in the RSF model, we identified age, gross tumor volume (GTV), grade, Karnofsky performance status (KPS), isocitrate dehydrogenase (IDH), and D99 as important variables associated with survival. The AUCs of the testing set were 92.4%, 87.7%, and 84.0% for 1-, 2-, and 3-year survival, respectively. According to this model, HGG patients can be divided into high- and low-risk groups.

Conclusion: The machine learning-based RSF model integrating both clinical and DVH variables is an improved and useful tool for predicting the survival of HGG patients.
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http://dx.doi.org/10.1002/cam4.3838DOI Listing
April 2021

Prognostic Risk Model of Immune-Related Genes in Colorectal Cancer.

Front Genet 2021 4;12:619611. Epub 2021 Mar 4.

Department of Colorectal Surgery and Oncology, Key Laboratory of Cancer Prevention and Intervention, Ministry of Education, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Purpose: We focused on immune-related genes (IRGs) derived from transcriptomic studies, which had the potential to stratify patients' prognosis and to establish a risk assessment model in colorectal cancer.

Summary: This article examined our understanding of the molecular pathways associated with intratumoral immune response, which represented a critical step for the implementation of stratification strategies toward the development of personalized immunotherapy of colorectal cancer. More and more evidence shows that IRGs play an important role in tumors. We have used data analysis to screen and identify immune-related molecular biomarkers of colon cancer. We selected 18 immune-related prognostic genes and established models to assess prognostic risks of patients, which can provide recommendations for clinical treatment and follow-up. Colorectal cancer (CRC) is a leading cause of cancer-related death in human. Several studies have investigated whether IRGs and tumor immune microenvironment (TIME) could be indicators of CRC prognoses. This study aimed to develop an improved prognostic signature for CRC based on IRGs to predict overall survival (OS) and provide new therapeutic targets for CRC treatment. Based on the screened IRGs, the Cox regression model was used to build a prediction model based on 18-IRG signature. Cox regression analysis revealed that the 18-IRG signature was an independent prognostic factor for OS in CRC patients. Then, we used the TIMER online database to explore the relationship between the risk scoring model and the infiltration of immune cells, and the results showed that the risk model can reflect the state of TIME to a certain extent. In short, an 18-IRG prognostic signature for predicting CRC patients' survival was firmly established.
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http://dx.doi.org/10.3389/fgene.2021.619611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970128PMC
March 2021

Apelin Rejuvenates Aged Human Mesenchymal Stem Cells by Regulating Autophagy and Improves Cardiac Protection After Infarction.

Front Cell Dev Biol 2021 2;9:628463. Epub 2021 Mar 2.

Faculty of Pharmacy, Bengbu Medical College, Bengbu, China.

The protective effects of mesenchymal stem cell (MSC)-based therapy for myocardial infarction (MI) are largely hampered as they age. Apelin is an endogenous ligand of its receptor APJ and plays an essential role in regulating multiple biological activities including MSC proliferation and survival. In this study, we investigated whether Apelin regulates MSC senescence and whether its overexpression could rejuvenate aged MSCs (AMSCs) to improve cardiac protection following infarction in mice. MSC senescence was evaluated by senescence-associated β-galactosidase assays. Apelin level was examined by western blotting. Autophagy was determined by transmission electron microscopy. The cardioprotective effect of AMSCs with Apelin overexpression (Apelin-AMSCs) was assessed in a mouse MI model. Apelin expression was dramatically reduced in AMSCs. Interestingly, knockdown of Apelin induced young MSCs (YMSC) senescence, whereas overexpression rescued AMSC senescence. Apelin overexpression also increased AMSC angiogenic capacity. Mechanistically, Apelin overexpression upregulated the autophagy level of AMSCs by activating AMP-activated protein kinase (AMPK) signaling, thereby rejuvenating AMSCs. Compared with AMSCs, transplantation of Apelin-AMSCs achieved better therapeutic efficacy for MI by enhancing cell survival and angiogenesis. In conclusion, our results reveal that Apelin activates AMPK to rejuvenate AMSCs by increasing autophagy and promotes cardioprotection following infarction in mice. This study identified a novel target to rejuvenate AMSCs and enhance their therapeutic efficacy.
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http://dx.doi.org/10.3389/fcell.2021.628463DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960672PMC
March 2021

Human Periodontal Ligament Stem Cells Transplanted with Nanohydroxyapatite/chitosan/gelatin 3D Porous Scaffolds Promote Jaw Bone Regeneration in Swine.

Stem Cells Dev 2021 Mar 19. Epub 2021 Mar 19.

Medical School of Nanjing University, 117559, Nanjing, Jiangsu, China;

Dental-tissue-derived stem cells have been used for tissue engineering due to their ease of isolation and efficacy in proliferation and differentiation in vitro and in vivo; and for bone tissue engineering scaffold, nanohydroxyapatite/chitosan/gelatin three-dimensional (3D) porous scaffold (nHA/CG) is promising for jaw bone regeneration because of its structural and functional similarity to natural bone. In our previous study,the efficiency of scaffolds with stem cells complexes on osteogenesis was confirmed in immunocompromised mice in vivo. However, the bone regeneration efficiency of stem cell-seeded nHA/CG scaffold in a large animal jaw bone defect model is unknown. The purpose of this study was to evaluate the bone regeneration potential of nHA/CG scaffold with human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs. hPDLSCs isolated from periodontal ligament of discarded teeth for orthodontic purpose were seeded into nHA/CG scaffold. The scaffold was successfully synthesized as described in our previous studies. Forty-eight critical-sized jaw bone defects were created in 12 minipigs. The surgical sites were randomly assigned to one of three groups to investigate the jaw bone regeneration of hPDLSCs composited with scaffold (hPDLSCs/nHA/CG), scaffold alone (nHA/CG) and negative control group with no cells and scaffold implanted into defects. The bone regeneration effects of three groups were accessed for up to 12 weeks. The results showed that hPDLSCs adhere well on nHA/CG scaffold in vitro, compared with pure scaffold, pure scaffold without cells, hPDLSCs and nHA/CG composities significantly increased new bone formation and generated large bone with normal architectures and vascularization in vivo. Immunohistochemistry showed that the runt-related transcription factor 2 (Runx2) were highly expressed in the bone marrow formed by hPDLSCs/nHA/CG. In conclusion, this study provided strong evidence for the future clinical applications of nHA/CG scaffold with transplanted hPDLSCs to regenerate bone in large jaw bone defects.
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http://dx.doi.org/10.1089/scd.2020.0204DOI Listing
March 2021

Dietary Trivalent Chromium Exposure Up-Regulates Lipid Metabolism in Coral Trout: The Evidence From Transcriptome Analysis.

Front Physiol 2021 25;12:640898. Epub 2021 Feb 25.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.

Diet quality greatly affects an animal's performance and metabolism. Despite the fact that trivalent chromium [Cr(III)] is considered an essential element and is widely used in nutritional supplements for animals and humans, the potential toxicity of Cr(III) is unclear. Here, liver transcriptome sequencing was performed on coral trout () exposed to 200 mg kg of dietary organic Cr(III) [as chromium picolinate (CrPic)] for 8 weeks. One-hundred-and thirteen differentially expressed genes (DEGs) were identified in response to Cr(III) stress, in comparison to the control, including 31 up-regulated and 82 down-regulated DEGs. Clusters of Orthologous Groups of proteins (COG) classifies DEGs into 15 functional categories, with the predominant category being related to lipid transport and metabolism (9.73%). The Kyoto Encyclopedia of Genes and Genomes (KEGG) assigned DEGs to six major categories with robust DEGs as part of the lipid metabolism pathway (18.58%). Moreover, KEGG functional enrichment analysis showed that these DEGs are primarily related to steroid biosynthesis, terpenoid backbone biosynthesis, and steroid hormone biosynthesis pathways, of which steroid biosynthesis was the most significant pathway, and 12 key up-regulated DEGs (, , and ) were found for steroid biosynthesis pathways. To validate the RNA sequencing data using quantitative real-time PCR (qRT-PCR), qRT-PCR results indicate that the expression of genes encoding HMGCR, TM7SF2, TRYP2, CTRL, EBP, LSS, and CYP51 were induced, while those encoding THRSP, LCE, and MCM5 were reduced, consistent with RNA-seq results. This findings provides the first evidence that a long-term high dose of Cr(III) intake causes lipid metabolism disorder and potential toxicity in fish. Cautious health risk assessment of dietary Cr(III) intake is therefore highly recommended for the commercial and/or natural diets of aquatic animals, which has previously largely been ignored.
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http://dx.doi.org/10.3389/fphys.2021.640898DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959734PMC
February 2021

CGM for insulinoma screening: a prospective and observational case-control study.

Endocr Relat Cancer 2021 Mar 1. Epub 2021 Mar 1.

J Han, Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Diabetes Institute, Shanghai Clinical Center for Diabetes, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Key Clinical Center for Metabolic Disease, Shanghai, China.

Insulin release index (IRI) based on 72-h fasting test has been used for the definitive diagnosis of insulinoma; however, hospitalization and subsequent costs contribute the disadvantage of IRI. Therefore, a simple and cost effective screening procedure on the diagnosis of the insulinoma for outpatients are urgent as needed. Continuous glucose monitoring (CGM) has been widely used for high level of glucose monitoring in diabetic patients. The aim of the study is to determine the potential contribution or implementation of CGM in the screening of the insulinoma. We performed a single center prospective study with the demographics and laboratory data including 28 patients with the pathological diagnosis of insulinoma and 25 patients with functional hypoglycemia as control group. The analysis showed that areas under the receiver operating characteristic (ROC) curve of coefficient of variation (CV) was 0.914. The CV cut-off point was 19% with the Youden 62.1%, the corresponding sensitivity and specificity were 82.1% and 80%, respectively. In patients with CV greater than the median, more than 60% of insulinomas were located in the head of the pancreas; most Ki-67 values were more than 2% and compared with the group with CV smaller than the median, the average tumor size was 2.7 times larger. In conclusion, CGM can be used as a valuable tool in not only monitoring high glucose levels in diabetic patients, but also identifying the etiology of insulinoma. CV greater than 19% can be highly effective for the screening of insulinoma in outpatients.
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http://dx.doi.org/10.1530/ERC-20-0447DOI Listing
March 2021

Effectiveness of a WeChat Combined Continuous Flash Glucose Monitoring System on Glycemic Control in Juvenile Type 1 Diabetes Mellitus Management: Randomized Controlled Trial.

Diabetes Metab Syndr Obes 2021 9;14:1085-1094. Epub 2021 Mar 9.

Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai, 200233, People's Republic of China.

Purpose: Smartphones have received increasing attention and achieved positive outcomes in diabetes intervention. The widespread use of WeChat in China provides an opportunity for self-management practices in patients with diabetes. Nevertheless, how to combine the strengths of the WeChat platform with traditional medical strategy remains to be explored. This study aimed to evaluate the efficacy of a novel flash glucose monitoring device combined with the WeChat platform in juvenile type 1 diabetes management.

Patients And Methods: A total of 60 juvenile patients with type 1 diabetes were randomly assigned into three groups: a blood glucose self-monitoring group (group A), a flash glucose monitoring (group B), and a flash glucose monitoring combined WeChat-interactive management group (group C). The intergroup differences in demographics, biochemical indicators, and questionnaire scores of the Diabetes Monitoring and Treatment Satisfaction Questionnaire and Diabetes Specific Quality of Life assessment were compared at the baseline and after 6 months.

Results: After the 6-month intervention, groups B and C showed significantly lower glycated hemoglobin A1c (HbA1c) levels compared to those observed at baseline (both <0.05), with the largest decrease observed in group C (group B vs group C, =0.04). Hypoglycemic episodes per month decreased from baseline in groups B and C (both P <0.05) and were more significant in group C ( <0.001). In addition, the DMTSQ scores increased in the 6th month in all groups (all <0.05), and the largest rise in scores was found in group C, followed by groups B and A. The DQOL scores in groups B and C decreased significantly from the baseline (both <0.05), with no change in group A.

Conclusion: Flash glucose monitoring combined with the WeChat-interactive system may help achieve sustained glycemic control and higher satisfaction in patients with juvenile type 1 diabetes.

Trial Registration: This study was registered at chictr.org.cn, number ChiCTR1900025495. Registered 29 August 2019.
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http://dx.doi.org/10.2147/DMSO.S299070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955684PMC
March 2021

Simultaneous control of intensity, phase, and polarization in real time under a weak oscillation theory.

Opt Lett 2021 Mar;46(6):1361-1364

Manipulating polarization, phase, and amplitude simultaneously in real time is an ultimate pursuit of controlling light. Several types of controllable metasurfaces have been realized, but with either low transmission efficiencies or limited control over amplitude, polarization, and phase in real time. Here we present a weak oscillation theory dealing with a new, to the best of our knowledge, type of optical system consisting of many layers of artificial oscillators, with each layer weakly interacting with the external field. As an application of our theory, we demonstrate and simulate a graphene-based metasurface structure to show that the oscillator system could change the focal length by changing the bias voltages. The polarization state to focus can also be selected by the bias voltage. The weak oscillation theory provides a flexible method to control the intensity, phase, and polarization.
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http://dx.doi.org/10.1364/OL.412851DOI Listing
March 2021

Effects of earthworm extract on the lipid profile and fatty liver induced by a high-fat diet in guinea pigs.

Ann Transl Med 2021 Feb;9(4):292

Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, China.

Background: Non-alcoholic fatty liver disease (NAFLD), characterized by the accumulation of excess fat in the liver in people who consume little or no alcohol, is becoming increasingly common around the world, especially in developed countries. Extracts from earthworms have been used as alternative therapies for a variety of diseases but not in NAFLD. Therefore, the aim of this study was to investigate the effect of earthworm extract (EE) on diet-induced fatty liver disease in guinea pigs.

Methods: EE was extracted, and the effect of EE on the lipid levels and liver damage in guinea pigs fed a high-fat diet (HFD) was assessed. Thirty male guinea pigs at 3 weeks of age were allocated equally to five groups, namely, chow diet, HFD, and HFD with different dosages (0.3, 1.4 and 6.8 µg per kg bodyweight per day) of EE for 4 weeks, and their body weight was monitored throughout the experiment. Liver tissues were examined for gross morphology and histology. Serum levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), alanine transaminase (ALT) and aspartate aminotransferase (AST) were determined using an autoanalyser.

Results: HFD induced NAFLD in guinea pigs. HFD-fed guinea pigs that received EE treatment showed milder increases in the serum levels of TC, TG and LDL-C, as well as in the body weight growth rate, compared to the HFD group without EE supplementation. EE intervention reduced the number of lipid-containing hepatocytes, hepatocellular ballooning and sinusoidal distortion in the liver in HFD-fed animals. ALT in serum was significantly elevated by HFD. No statistically significant difference in ALT levels was found between the chow diet group and the HFD group with EE treatment.

Conclusions: This study demonstrates that the administration of EE suppressed the induction of serum TC, TG and LDL-C in response to HFD. EE also reduced liver damage in HFD-fed guinea pigs. These findings suggest that EE has alleviating effects on dyslipidaemia and liver damage associated with NAFLD.
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http://dx.doi.org/10.21037/atm-20-5362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944315PMC
February 2021

High-frequency rectifiers based on type-II Dirac fermions.

Nat Commun 2021 Mar 11;12(1):1584. Epub 2021 Mar 11.

State Key Laboratory for Infrared Physics, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai, China.

The advent of topological semimetals enables the exploitation of symmetry-protected topological phenomena and quantized transport. Here, we present homogeneous rectifiers, converting high-frequency electromagnetic energy into direct current, based on low-energy Dirac fermions of topological semimetal-NiTe, with state-of-the-art efficiency already in the first implementation. Explicitly, these devices display room-temperature photosensitivity as high as 251 mA W at 0.3 THz in an unbiased mode, with a photocurrent anisotropy ratio of 22, originating from the interplay between the spin-polarized surface and bulk states. Device performances in terms of broadband operation, high dynamic range, as well as their high sensitivity, validate the immense potential and unique advantages associated to the control of nonequilibrium gapless topological states via built-in electric field, electromagnetic polarization and symmetry breaking in topological semimetals. These findings pave the way for the exploitation of topological phase of matter for high-frequency operations in polarization-sensitive sensing, communications and imaging.
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http://dx.doi.org/10.1038/s41467-021-21906-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952558PMC
March 2021

Glycemic Trajectories After Tuberculosis Diagnosis and Treatment Outcomes of New Tuberculosis Patients: A Prospective Study in Eastern China.

Am J Respir Crit Care Med 2021 Mar 11. Epub 2021 Mar 11.

Stanford University School of Medicine, 10624, Division of Infectious Diseases and Geographic Medicine, Stanford, California, United States.

Rationale Newly diagnosed tuberculosis patients often have inconsistent glycemic measurements during and after treatment. Distinct glycemic trajectories after tuberculosis diagnosis are not well characterized and whether patients with stress hyperglycemia have poor treatment outcomes is not known. Objectives To identify distinct glycemic trajectories from tuberculosis diagnosis to post-treatment and to assess the relationship between glycemic trajectories and tuberculosis treatment outcomes. Methods Newly diagnosed, drug-susceptible tuberculosis patients with at least three fasting plasma glucose (FPG) tests at tuberculosis diagnosis and during the 3rd and 6th month of treatment were identified and included from Jiangsu Province, China. Patients were also given an additional FPG test at two and four months post-treatment. Measurements and Main Results Several distinct glycemic trajectories from tuberculosis diagnosis to post-treatment were found including consistently normal glycemic testing (43%), transient hyperglycemia (24%), erratic glycemic instability (12%), diabetes (16%), and consistently hyperglycemic but without diabetes (6%). Compared to participants with a consistently normal glycemic trajectory, patients were more likely to fail treatment if they had transient hyperglycemia (Adjusted Odds Ratio [AOR], 4.20; 95% CI, 1.57-11.25, P=0.004) or erratic glycemic instability (AOR, 5.98; 95% CI, 2.00-17.87; P=0.001). Patients living with diabetes also had higher risk of treatment failure (AOR, 6.56; 95% Confidence Interval [CI], 2.22-19.35, P=0.001), and this was modified by glycemic control and metformin use. Conclusions Among tuberculosis patients without diabetes, glycemic changes were common and may represent an important marker for patient response to tuberculosis treatment.
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http://dx.doi.org/10.1164/rccm.202007-2634OCDOI Listing
March 2021

Assessment of the structured clinical interview (SCID) for DSM-5 for somatic symptom disorder in general hospital outpatient clinics in China.

BMC Psychiatry 2021 Mar 10;21(1):144. Epub 2021 Mar 10.

Institute of Psychology, University of Freiburg, Freiburg im Breisgau, Germany.

Background: It is still unknown whether the "Somatic symptom disorders (SSD) and related disorders" module of the Structured Clinical Interview for DSM-5, research version (SCID-5-RV), is valid in China. This study aimed to assess the SCID-5-RV for SSD in general hospital outpatient clinics in China.

Methods: This multicentre cross-sectional study was conducted in the outpatient clinics of nine tertiary hospitals in Beijing, Jincheng, Shanghai, Wuhan, and Chengdu between May 2016 and March 2017. The "SSD and related disorders" module of the SCID-5-RV was translated, reversed-translated, revised, and used by trained clinical researchers to make a diagnosis of SSD. Several standardized questionnaires measuring somatic symptom severity, emotional distress, and quality of life were compared with the SCID-5-RV.

Results: A total of 699 patients were recruited, and 236 were diagnosed with SSD. Of these patients, 46 had mild SSD, 78 had moderate SSD, 100 had severe SSD, and 12 were excluded due to incomplete data. The SCID-5-RV for SSD was highly correlated with somatic symptom severity, emotional distress, and quality of life (all P < 0.001) and could distinguish nonsevere forms of SSD from severe ones.

Conclusions: This study suggests that SCID-5-RV for SSD can distinguish SSD from non-SSD patients and severe cases from nonsevere cases. It has good discriminative validity and reflects the DSM-5 diagnostic approach that emphasizes excessive emotional, thinking, and behavioural responses related to symptoms.
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http://dx.doi.org/10.1186/s12888-021-03126-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944631PMC
March 2021

Characterization of an Antiviral Component in Human Seminal Plasma.

Front Immunol 2021 19;12:580454. Epub 2021 Feb 19.

Institute of Basic Medical Sciences, School of Basic Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Numerous types of viruses have been found in human semen, which raises concerns about the sexual transmission of these viruses. The overall effect of semen on viral infection and transmission have yet to be fully investigated. In the present study, we aimed at the effect of seminal plasma (SP) on viral infection by focusing on the mumps viral (MuV) infection of HeLa cells. MuV efficiently infected HeLa cells . MuV infection was strongly inhibited by the pre-treatment of viruses with SP. SP inhibited MuV infection through the impairment of the virus's attachment to cells. The antiviral activity of SP was resistant to the treatment of SP with boiling water, Proteinase K, RNase A, and DNase I, suggesting that the antiviral factor would not be proteins and nucleic acids. PNGase or PLA2 treatments did not abrogate the antiviral effect of SP against MuV. Further, we showed that the prostatic fluid (PF) showed similar inhibition as SP, whereas the epididymal fluid and seminal vesicle extract did not inhibit MuV infection. Both SP and PF also inhibited MuV infection of other cell types, including another human cervical carcinoma cell line C33a, mouse primary epididymal epithelial cells, and Sertoli cell line 15P1. Moreover, this inhibitory effect was not specific to MuV, as the herpes simplex virus 1, dengue virus 2, and adenovirus 5 infections were also inhibited by SP and PF. Our findings suggest that SP contains a prostate-derived pan-antiviral factor that may limit the sexual transmission of various viruses.
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http://dx.doi.org/10.3389/fimmu.2021.580454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933687PMC
February 2021

Improved outcomes using unmanipulated haploidentical hematopoietic stem cells combined with third-party umbilical cord blood transplantation for non-malignant diseases in children: The experience of a single center.

Pediatr Transplant 2021 Mar 6:e13995. Epub 2021 Mar 6.

Department of Pediatrics, Chinese PLA General Hospital-Sixth Medical Center, Beijing, China.

Background: Unmanipulated haploid HSCT for SAA has resulted in improved outcomes over recent years. However, studies related to unmanipulated haploid HSCs combined with tp-UCB transplantation for other types of NMD are rare. Accordingly, we present the outcomes of 109 pediatric patients with life-threatening NMD undergoing unmanipulated haploid HSCs combined with tp-UCB transplantation.

Procedure: We retrospectively investigated 109 pediatric patients with life-threatening NMD treated with unmanipulated haploid HSCs combined with tp-UCB transplantation in a single center.

Results: The median days of neutrophil and platelet engraftment were +13 and +22 days, respectively. None of the cases experienced PGF. The incidence rates for grade I-II, III-IV aGVHD and cGVHD were 44.9%, 24.8%, and 9.3%, respectively. The incidence rates of CMV and EBV viremia were 46.7% and 39.4%, respectively. The median follow-up duration was 997 days. In total, 106 patients survived, including 104 cases with FFS and 2 cases with SGF. Three patients died. The 5-year TRM, OS, and FFS were 2.8%, 97.2%, and 96.2%, respectively.

Conclusion: The results of unmanipulated haploid HSCs combined with tp-UCB in pediatric patients with life-threatening NMD were promising. However, further research is now needed to determine specific factors that might influence the engraftment of HSCs.
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http://dx.doi.org/10.1111/petr.13995DOI Listing
March 2021