Publications by authors named "Lu Li"

2,771 Publications

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Microstructured BN composites with internally designed high thermal conductivity paths for 3D electronic packaging.

Adv Mater 2022 Aug 9:e2205120. Epub 2022 Aug 9.

School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.

Miniaturized and high-power density 3D electronic devices pose new challenges on thermal management. Indeed, prompt heat dissipation in electrically insulating packaging is currently limited by the thermal conductivity achieved by thermal interface materials (TIMs) and by their capability to direct the heat towards heat sinks. Here, we create high thermal conductivity BN-based composites able to conduct heat intentionally towards specific areas by locally orienting magnetically functionalized BN microplatelets using magnetically assisted slip casting (MASC). The obtained thermal conductivity along the direction of alignment is unusually high, up to 12.1 W m K thanks to a high concentration of 62.6 vol% of BN in the composite, a low concentration in polymeric binder and a high degree of alignment. The BN composites have a low density of 1.3 g cm , a high stiffness of 442.3 MPa and are electrically insulating. Uniquely, we demonstrate our approach with proof-of-concept composites having locally graded orientations of BN microplatelets to direct the heat away from two vertically stacked heat sources. Rationally designing the microstructure of TIMs to direct heat strategically provides a promising solution for efficient thermal management in 3D integrated electronics. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202205120DOI Listing
August 2022

Sequestered SQSTM1/p62 crosstalk with Keap1/NRF2 axis in hPDLCs promotes oxidative stress injury induced by periodontitis.

Free Radic Biol Med 2022 Aug 5. Epub 2022 Aug 5.

Department of Periodontology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China; Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China. Electronic address:

Periodontitis is a recognized multifactorial inflammatory chronic disease, however, the exact role of oxidative stress in the pathogenesis of periodontitis is undefined. This study aims to imply the mechanism of NRF2-regulated oxidative stress and inflammatory responses under periodontitis and explored the novelty therapeutic targets. We first demonstrate that redox imbalance caused by inhibited NRF2 signaling pathway is induced in periodontium during hypoxia and bacterial events. Then we propose that LPS from P. gingivalis and hypoxia stimuli could inhibit hPDLCs proliferation and GSH level, promote ROS production, lipid peroxidation level, and pro-inflammatory cytokines such as IL-6, TNF-α, and IL-17 level caused by the inhibited PI3K/AKT/mTOR pathway and sequential sequestered crosstalk between selective autophagy SQSTM1/p62 and Keap1/NRF2 axis accompanied by the reinforced NRF2 ubiquitination degradation and inactivated NRF2 nuclear translocation. Overexpression of NRF2 and SQSTM1 can protect hPDLCs from oxidative stress and inflammation exacerbation because of enhanced NRF2 activity. Further, the antioxidant and anti-inflammation potential of puerarin is verified in vitro and in experimental periodontitis in mice through diminishing above negative feedback loop mechanically. Altogether, we speculate that NRF2-mediated redox homeostasis is a profound candidate for one of the prominent roles in periodontitis pathogenesis and suggest puerarin as a promising therapeutic target.
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http://dx.doi.org/10.1016/j.freeradbiomed.2022.08.001DOI Listing
August 2022

Organic chromium derived from the chelation of Ganoderma lucidum polysaccharide and chromium (III) alleviates metabolic syndromes and intestinal microbiota dysbiosis induced by high-fat and high-fructose diet.

Int J Biol Macromol 2022 Aug 5. Epub 2022 Aug 5.

Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China. Electronic address:

Organic chromium is of great interest and has become an important chromium supplement resource in recent years because of its low toxicity and easy absorption. In our previous study, we synthesized a novel organic chromium [GLP-Cr] through the chelation of Ganoderma lucidum polysaccharide and chromium (III). The purpose of this study was to investigate the beneficial effects of GLP-Cr on the improvement of metabolic syndromes (MetS) in mice fed with a high-fat and high-fructose diet (HFHFD) and its mechanism of action. The results indicated that oral administration of GLP-Cr inhibited the excessive exaltation of body weight, glucose tolerance, fasting blood glucose and lipid levels, hepatic total cholesterol (TC), triglyceride (TG) levels caused by HFHFD. Besides, 16S rRNA amplicon sequencing showed that GLP-Cr intervention evidently ameliorated intestinal microbiota dysbiosis by changing the proportions of some intestinal microbial phylotypes. In addition, correlation network-based analysis indicated that the key intestinal microbial phylotypes were closely related to biochemical parameters associated with MetS under GLP-Cr intervention. Liver metabolomics analysis suggested that GLP-Cr intervention significantly regulated the levels of some biomarkers involved in alpha-linolenic acid metabolism, fatty acid biosynthesis, steroid hormone biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, steroid hormone biosynthesis, primary bile acid biosynthesis, and so on. Moreover, GLP-Cr intervention regulated liver mRNA levels of key genes associated with glucose and lipid metabolism. The mRNA level of glucose transporter type 4 (Glut4) was markedly increased by GLP-Cr intervention, and the mRNA levels of phosphoenolpyruvate carboxykinase (Pepck) and glucose-6-phosphatase (G6Pase) in the liver were significantly decreased. Meanwhile, GLP-Cr intervention significantly decreased hepatic mRNA levels of cluster of differentiation 36 (Cd36), acetyl-CoA carboxylase 1 (Acc1) and sterol regulatory element binding protein-1c (Srebp-1c), indicating that GLP-Cr intervention inhibited the excessive accumulation of free fatty acids in the liver. These findings suggest that the prevention of hyperglycemia and dyslipidemia by GLP-Cr may be closely related to the regulation of gut microbial composition and hepatic metabolic pathways, thus GLP-Cr can be serving as a functional component in the prevention of MetS.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.211DOI Listing
August 2022

Modulation of Disordered Bile Acid Homeostasis and Hepatic Tight Junctions Using Salidroside against Hepatocyte Apoptosis in Furan-Induced Mice.

J Agric Food Chem 2022 Aug 8. Epub 2022 Aug 8.

College of Food Science and Engineering, Jilin University, Changchun 130062, China.

Furan, a processing-induced food contaminant, has attracted great attention due to its hepatotoxicity. To further investigate the underlying mechanism of salidroside (SAL) alleviating furan-induced liver damage, we divided Balb/c mice into the control group, the furan (8 mg/kg/day) group, and three groups of three different doses of SAL (10/20/40 mg/kg/day) in the current research. The shifted serum profile was observed through untargeted metabonomics, to which the bile acid metabolism was related, and the alleviating effect of SAL against furan-induced apoptosis was caused by the metabolism. Target bile acid quantification for the liver and serum showed that SAL positively regulated the homeostasis of bile acids disturbed by furan. Meanwhile, SAL significantly upregulated the synthesis genes of bile acids (, , , and ) and the uptake transport genes ( and ) and downregulated the efflux transport genes (, , , , and 4). Transmission electron microscopy of the bile canaliculi and tight junctions and the levels of tight junction marker proteins (ZO-1, occludin, and claudin-1) confirmed that the disruption of the hepatic tight junction was inhibited by SAL. The connection between the apoptosis- and tight junction-related proteins was observed through the construction of a protein-protein interaction network. SAL suppressed the furan-induced hepatocyte apoptosis evidenced by the detection of TUNEL and Bax, Bcl-2, and caspase-3 levels. Taken together, SAL alleviated furan-induced hepatocyte apoptosis via altering the disordered homeostasis of bile acids and hepatic tight junctions.
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http://dx.doi.org/10.1021/acs.jafc.2c04654DOI Listing
August 2022

The Metabolic Adaptation in Response to Nitrate Is Critical for Actinobacillus pleuropneumoniae Growth and Pathogenicity under the Regulation of NarQ/P.

Infect Immun 2022 Aug 8:e0023922. Epub 2022 Aug 8.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural Universitygrid.35155.37, Wuhan, Hubei, China.

Nitrate metabolism is an adaptation mechanism used by many bacteria for survival in anaerobic environments. As a by-product of inflammation, nitrate is used by the intestinal bacterial pathogens to enable gut infection. However, the responses of bacterial respiratory pathogens to nitrate are less well understood. Actinobacillus pleuropneumoniae is an important bacterial respiratory pathogen of swine. Previous studies have suggested that adaptation of A. pleuropneumoniae to anaerobiosis is important for infection. In this work, A. pleuropneumoniae growth and pathogenesis in response to the nitrate were investigated. Nitrate significantly promoted A. pleuropneumoniae growth under anaerobic conditions and lethality in mice. By using and deletion mutants and single-residue-mutated complementary strains of Δ, the two-component system NarQ/P was confirmed to be critical for nitrate-induced growth, with Arg50 in NarQ as an essential functional residue. Transcriptome analysis showed that nitrate upregulated multiple energy-generating pathways, including nitrate metabolism, mannose and pentose metabolism, and glycerolipid metabolism via the regulation of NarQ/P. Furthermore, , , and its target gene encoding the nitrate reductase Nap contributed to the pathogenicity of A. pleuropneumoniae. The Nap inhibitor tungstate significantly reduced the survival of A. pleuropneumoniae , suggesting that Nap is a potential drug target. These results give new insights into how the respiratory pathogen A. pleuropneumoniae utilizes the alternative electron acceptor nitrate to overcome the hypoxia microenvironment, which can occur in the inflammatory or necrotic infected tissues.
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http://dx.doi.org/10.1128/iai.00239-22DOI Listing
August 2022

Metagenomic profiling of ocular surface microbiome changes in blepharitis patients.

Front Cell Infect Microbiol 2022 22;12:922753. Epub 2022 Jul 22.

School of Optometry and Ophthalmology, the Eye Hospital of Wenzhou Medical University, Wenzhou, China.

Purpose: To compare the ocular surface and meibum microbial communities of humans with Blepharitis (DB) and healthy controls.

Methods: Conjunctival sac and meibum samples from 25 DB patients and 11 healthy controls were analyzed using metagenomic next-generation sequencing (mNGS).

Results: The alpha-diversity of the conjunctival sac microbiome of the group (observed, Chao1, ACE) was lower than that of the control group, whereas all meibum diversity indicators were similar. In conjunctival samples, the relative abundance (RA) of the phylum Proteobacteria was significantly higher (=0.023), and the RA of both phyla Actinobacteria and Firmicutes was significantly lower (=0.002, 0.025, respectively) in the DB group than that in the control group. In meibum samples, the RA of the phyla Proteobacteria and Actinobacteria were similar, whereas that of the phylum Firmicutes was significantly lower in the DB group (=0.019) than that in the control group. Linear discriminant analysis with effect size measurement of the conjunctival and meibum microbiomes showed that sp. and were enriched in the DB group. sp. and in the DB group were related to more severe ocular surface clinical parameters. Discriminative genera's principal coordinate analysis separated all control and DB microbiomes into two distinct clusters.

Conclusions: Proteobacteria's increased prevalence may indicate ocular microbial community instability. The species sp. and are potentially pathogenic bacterial biomarkers in DB. infection mainly affects the ocular surface microbiome rather than penetrating deeper into the meibomian gland.
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http://dx.doi.org/10.3389/fcimb.2022.922753DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354880PMC
August 2022

Fertility intentions, parenting attitudes and fear of childbirth among college students in China: A cross-sectional study.

J Pediatr Adolesc Gynecol 2022 Aug 3. Epub 2022 Aug 3.

School of Nursing, Wuhan University, Wuhan, China. Electronic address:

Study Objective: This study aimed to investigate the current situation regarding fertility intentions, parenting attitudes and fear of childbirth among college students in mainland China, and the factors related to these variables.

Design: A cross-sectional study.

Setting: Colleges across China.

Participants: 583 college students attending regular institutions of higher education.

Main Outcome Measures: Fertility intentions and fear of childbirth were measured using the Swedish Fertility Awareness Questionnaire and the Childbirth Fear Prior to Pregnancy (CFPP) scale.

Results: Only 38.8% of participants expressed their willingness to have children, and there was a significant difference between male and female students (P < 0.02). Males regarded having children as more important (P < 0.01), and females were more concerned about the negative effects of becoming parents, including the labor market, having less freedom and a reduced economy. When deciding whether to have children, factors such as work, economics and childcare were more important to females. Students who did not want children had higher levels of fear of childbirth than those who want children or were unsure (P < 0.02).

Conclusions: The fertility intentions of college students were not optimistic. In addition to the two-child Chinese fertility policy, interventions aimed at reducing the cost of raising children, eliminating gender inequality in the workplace, and normalizing child-care institutions might help alleviate conflict between work and child rearing.
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http://dx.doi.org/10.1016/j.jpag.2022.07.015DOI Listing
August 2022

Characterization of primary canine Sertoli cells as a model to test male reproductive toxicant.

Toxicol In Vitro 2022 Aug 2:105452. Epub 2022 Aug 2.

Reprotox Biotech LLC, 800 Bradbury, Drive, SE, Science &Technology Park, Albuquerque, NM 87106, United States of America. Electronic address:

Sertoli cells play critical roles in regulating spermatogenesis and testis development by providing structural and nutritional support. This study aimed to develop a standard protocol for canine Sertoli cell isolation and culture; and characterize its biological features, functionality, and application of compound toxicity testing. Canine testicles were received from the neuter clinic, and three-step of enzymatic digestion was applied to isolate Sertoli cells. We characterized the growth and purity of Sertoli cells with the expression of SOX9, GATA4, and Clusterin. In addition, we selected cadmium as a model toxicant to evaluate the toxic responses in the newly established Sertoli cells using High-content Analysis (HCA). With our optimized protocol, the purity of isolated Sertoli cells was above 95%, as determined with Sertoli cell-specific protein markers of SOX9 and GATA4. More importantly, primary Sertoli cell populations could be expanded rapidly in vitro, passaged (up to seven), and cryopreserved. The HCA-based assay revealed that cadmium at 1 μM induced both disruptions of cytoskeletal and DNA damage responses. Furthermore, we established an HCA assay with the newly isolated and optimized culture of canine Sertoli cells to evaluate the epigenetic markers of histone modification. We found cadmium-induced differential changes in histone modifications H3Me3K9, H3Me3K36, H4Me3K20, and H4acK5. In summary, we have established the standardized protocol to produce canine Sertoli cells with Sertoli cell-specific phenotype. The isolation and expansion of large quantities of canine Sertoli cells will provide broad applications in studying male infertility, reproductive toxicology, testicular cancer, and cell therapy.
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http://dx.doi.org/10.1016/j.tiv.2022.105452DOI Listing
August 2022

Intrauterine neuromuscular and stromal dysplasia of the bladder in retinoic acid-induced myelomeningocele fetal rats.

Tissue Cell 2022 Jul 22;78:101872. Epub 2022 Jul 22.

Children's Hospital of Soochow University, Suzhou, Jiangsu, China. Electronic address:

Objectives: To observe the changes in the bladder of fetal rats with myelomeningocele (MMC) induced by all-trans retinoic acid (atRA) during the embryonic development stages.

Methods: The fetal rat model of MMC was induced by intragastric administration of atRA to pregnant rats on embryonic day 10 (E10). Fetal rats were harvested at E16, E18, E20, and E21 for observation and further testing. Those with MMC were classified as the MMC group, while those without MMC as the RA group. The areas of MMC skin defect, the crown-rump length (CRL), and body weight in different groups were compared. The histopathological changes in the bladder were compared. The expression levels of alpha-smooth muscle actin (αSMA), smooth muscle myosin heavy chain (SMMHC), connexin 43 (Cx43), desmin, β3 tubulin, and vesicular acetylcholine transporter (VAChT) in the bladder were investigated by immunohistochemical staining and Western blotting. Pregnant rats given intragastric administration with olive oil (OIL group) at E10 were set as the blank control group.

Results: A total of 415 fetal rats of different gestational ages were harvested with an MMC incidence of 56.05 % (139/248). The incidence rate increased with embryonic days (p < 0.001). Compared with the other two control groups, the CRL and bodyweight of MMC fetal rats were significantly delayed at E21 (p < 0.001). The expression levels of αSMA, SMMHC, Cx43, desmin, β3 tubulin and VAChT in the bladder of MMC fetal rats were significantly decreased at E21 (p < 0.05).

Conclusions: In atRA-induced MMC fetal rats, there is neural, muscular, and stromal dysplasia in the bladder at an early gestational age. Further investigations on neurogenic bladder secondary to MMC are applicable using this animal model.
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http://dx.doi.org/10.1016/j.tice.2022.101872DOI Listing
July 2022

Computational approach to modeling microbiome landscapes associated with chronic human disease progression.

PLoS Comput Biol 2022 Aug 4;18(8):e1010373. Epub 2022 Aug 4.

Department of Computer Science and Engineering, University at Buffalo, The State University of New York, Buffalo, New York, United States of America.

A microbial community is a dynamic system undergoing constant change in response to internal and external stimuli. These changes can have significant implications for human health. However, due to the difficulty in obtaining longitudinal samples, the study of the dynamic relationship between the microbiome and human health remains a challenge. Here, we introduce a novel computational strategy that uses massive cross-sectional sample data to model microbiome landscapes associated with chronic disease development. The strategy is based on the rationale that each static sample provides a snapshot of the disease process, and if the number of samples is sufficiently large, the footprints of individual samples populate progression trajectories, which enables us to recover disease progression paths along a microbiome landscape by using computational approaches. To demonstrate the validity of the proposed strategy, we developed a bioinformatics pipeline and applied it to a gut microbiome dataset available from a Crohn's disease study. Our analysis resulted in one of the first working models of microbial progression for Crohn's disease. We performed a series of interrogations to validate the constructed model. Our analysis suggested that the model recapitulated the longitudinal progression of microbial dysbiosis during the known clinical trajectory of Crohn's disease. By overcoming restrictions associated with complex longitudinal sampling, the proposed strategy can provide valuable insights into the role of the microbiome in the pathogenesis of chronic disease and facilitate the shift of the field from descriptive research to mechanistic studies.
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http://dx.doi.org/10.1371/journal.pcbi.1010373DOI Listing
August 2022

Niche differentiation of atmospheric methane-oxidizing bacteria and their community assembly in subsurface karst caves.

Environ Microbiol Rep 2022 Aug 4. Epub 2022 Aug 4.

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, P. R. China.

Karst caves are recently proposed as atmospheric methane sinks in terrestrial ecosystems. Despite of the detection of atmospheric methane-oxidizing bacteria (atmMOB) in caves, we still know little about their ecology and potential ability of methane oxidation in this ecosystem. To understand atmMOB ecology and their potential in methane consumption, we collected weathered rocks and sediments from three different caves in southwestern China. We determined the potential methane oxidization rates in the range of 1.25 ± 0.08 to 1.87 ± 0.41 ng CH  g DW h , which are comparable to those reported in forest and grassland soils. Results showed that alkaline oligotrophic caves harbour high numbers of atmMOB, particularly upland soil cluster (USC), which significantly correlated with temperature, CH and CO concentrations. The absolute abundance of USCγ was higher than that of USCα. USCγ-OPS (open patch soil) and USCγ-SS (subsurface soil) dominated in most samples, whereas USCα-BFS (boreal forest soil) only predominated in the sediments near cave entrances, indicating niche differentiation of atmMOB in caves. Overwhelming dominance of homogenous selection in community assembly resulted in convergence of atmMOB communities. Collectively, our results demonstrated the niche differentiation of USC in subsurface alkaline caves and their non-negligible methane-oxidizing potential, providing brand-new knowledge about atmMOB ecology in subsurface biosphere.
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http://dx.doi.org/10.1111/1758-2229.13112DOI Listing
August 2022

Effectiveness and safety of low-dose interferon alpha-2a treatment in Behçet's Syndrome with refractory vascular or neurological involvement: a case series.

Ther Adv Chronic Dis 2022 26;13:20406223221111285. Epub 2022 Jul 26.

Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Dongcheng-qu, Beijing 100730, China.

Objective: The aim of this study was to evaluate the effectiveness and safety of low-dose interferon alpha-2a (IFNα2a) in Behçet's syndrome (BS) patients with refractory vascular/cardiac or neurological involvement.

Methods: In this retrospective cohort study, we consecutively included 25 BS patients with refractory vascular/cardiac ( = 16) or neurological involvement ( = 9) who received IFNα2a treatment in our center between June 2018 and September 2021. The low-dose IFNα2a (3 million IU, every other day) was used as an add-on treatment with the continuation of glucocorticoids (GCs) and immunosuppressants.

Results: In total, 25 patients (20 males, 5 females) with a mean age of 31.92 ± 9.25 years were included. IFNα2a was administered for BS patients with refractory vascular/cardiac involvement ( = 16) and neurological involvement ( = 9). Before the initiation of IFNα2a, patients had insufficient response or intolerance to conventional therapies. After a median follow-up of 23 [interquartile range (IQR), 11-30] months, all patients achieved clinical improvement. The Behçet's disease Current Activity Form (BDCAF) score improved significantly (5 0, median,  < 0.0001). BS Overall Damage Index (BODI) and vasculitis damage index (VDI) remain stable ( > 0.05). Decrease in erythrocyte sedimentation rate [ESR; 24 (IQR, 12-43.5) 5 (IQR, 2.75-10.5) mm/h,  = 0.0001] and C-reactive protein [CRP; 6.64 (IQR, 3.67-19.82) 1.24 (IQR, 0.24-3.12) mg/liter,  < 0.005] was achieved effectively. The median GCs dosage tapered from 26.25 (IQR, 11.88-41.25) to 10.00 (IQR, 7.50-10.63) mg/d,  < 0.0001. Immunosuppressants were also reduced in number ( < 0.005). No serious adverse events were observed during follow-up.

Conclusion: Our study suggests that low-dose IFNα2a, combined with GCs and immunosuppressants, is well-tolerated and effective for BS patients with refractory vascular/cardiac or neurological involvement and has a steroid- and immunosuppressant-sparing effect.
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http://dx.doi.org/10.1177/20406223221111285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340357PMC
July 2022

Proteotypic Differences of Follicular-Patterned Thyroid Neoplasms.

Front Endocrinol (Lausanne) 2022 6;13:854611. Epub 2022 Jul 6.

The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

The diagnosis of follicular-patterned thyroid tumors such as follicular thyroid adenoma (FA), follicular thyroid carcinoma (FTC), and follicular variant of papillary thyroid carcinoma (FvPTC) remains challenging. This study aimed to explore the molecular differences among these three thyroid tumors by proteomic analysis. A pressure cycling technology (PCT)-data-independent acquisition (DIA) mass spectrometry workflow was employed to investigate protein alterations in 52 formalin-fixed paraffin-embedded (FFPE) specimens: 18 FA, 15 FTC, and 19 FvPTC specimens. Immunohistochemical (IHC) analysis of 101 FA, 67 FTC, and 65 FvPTC specimens and parallel reaction monitoring (PRM) analysis of 20 FA, 20 FTC, and 20 FvPTC specimens were performed to validate protein biomarkers. A total of 4107 proteins were quantified from 52 specimens. Pairwise comparisons identified 287 differentially regulated proteins between FTC and FA, and 303 between FvPTC and FA and 88 proteins were co-dysregulated in the two comparisons. However, only 23 discriminatory proteins between FTC and FvPTC were detected. Additionally, the quantitative results for ANXA1 expression based on IHC staining and PRM-MS quantification were consistent with the proteomic results, showing that ANXA1 can be used to distinguish FvPTC from FA and FTC. The differentially regulated proteins found in this study can differentiate FA from FvPTC. In addition, ANXA1 is a promising biomarker for differentiating FvPTC from the other thyroid tumors.
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http://dx.doi.org/10.3389/fendo.2022.854611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340356PMC
August 2022

Partial Linker Substitution Strategy to Construct a Quaternary HKUST-like MOF for Efficient Acetylene Storage and Separation.

ACS Appl Mater Interfaces 2022 Aug 3. Epub 2022 Aug 3.

Fujian Provincial Key Laboratory of Polymer Materials, College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, P. R. China.

Multicomponent metal-organic frameworks (MOFs) have received much attention as emerging materials capable of precisely programing exquisite structures and specific functions. Here, we applied a partial linker substitution strategy to compile an HKUST-1-like quaternary MOF by introducing a bifunctional ligand into the well-known HKUST-1 structure. , a new HKUST-like topology MOF, was assembled with paddlewheel [Cu(COO)], triangular metallocycle pyrazole cluster Cu(μ-OH) (NN) building blocks, and two distinct linkers. exhibited good mechanical stability, water stability, and chemical stability (pH = 3-12) in aqueous solutions. Moreover, the porous environments created by this multicomponent primitive endow with high CH storage and significantly selective separation performance of CH/CO. Dynamic breakthrough experiments and ideal adsorbed solution theory calculations further demonstrate that can selectively capture CH from CH/CO mixtures under ambient conditions. Based on grand canonical Monte Carlo simulations, the high CH separation performance of is attributed to the π-complex formed between the CH molecule and the trinuclear metallocycle clusters on the wall, which provides stronger affinity for CH recognition than the CO molecule.
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http://dx.doi.org/10.1021/acsami.2c10346DOI Listing
August 2022

Rif1 interacts with non-canonical polycomb repressive complex PRC1.6 to regulate mouse embryonic stem cells fate potential.

Cell Regen 2022 Aug 2;11(1):25. Epub 2022 Aug 2.

Hunan Key Laboratory of Molecular Precision Medicine, Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Mouse embryonic stem cells (mESCs) cycle in and out of a transient 2-cell (2C)-like totipotent state, driven by a complex genetic circuit involves both the coding and repetitive sections of the genome. While a vast array of regulators, including the multi-functional protein Rif1, has been reported to influence the switch of fate potential, how they act in concert to achieve this cellular plasticity remains elusive. Here, by modularizing the known totipotency regulatory factors, we identify an unprecedented functional connection between Rif1 and the non-canonical polycomb repressive complex PRC1.6. Downregulation of the expression of either Rif1 or PRC1.6 subunits imposes similar impacts on the transcriptome of mESCs. The LacO-LacI induced ectopic colocalization assay detects a specific interaction between Rif1 and Pcgf6, bolstering the intactness of the PRC1.6 complex. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) analysis further reveals that Rif1 is required for the accurate targeting of Pcgf6 to a group of genomic loci encompassing many genes involved in the regulation of the 2C-like state. Depletion of Rif1 or Pcgf6 not only activates 2C genes such as Zscan4 and Zfp352, but also derepresses a group of the endogenous retroviral element MERVL, a key marker for totipotency. Collectively, our findings discover that Rif1 can serve as a novel auxiliary component in the PRC1.6 complex to restrain the genetic circuit underlying totipotent fate potential, shedding new mechanistic insights into its function in regulating the cellular plasticity of embryonic stem cells.
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http://dx.doi.org/10.1186/s13619-022-00124-9DOI Listing
August 2022

Fabrication, characterization, and purification of nutraceutical diacylglycerol components from Camellia oil.

J Food Sci 2022 Jul 29. Epub 2022 Jul 29.

The Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.

Converting triacylgycerols (TAGs) from edible oils and fats into structured diacylglycerols (DAGs) is meaningful for reducing obesity. Camellia oil, rich in linoleic acid, has the potential to form structured linoleic acid-1,3-diacylglycerol (LA-1,3-DAG) nutrients in the industry. In this research, the physicochemical properties of modified Camellia oil (MCO) by enzymatic esterification were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS), Differential Scanning Calorimetry (DSC), High Performance Liquid Chromatography-Evaporative Light Scattering Detection (HPLC-ELSD), and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS). The relationship between reaction conditions and the DAG compositions is disclosed using multiple factors. It is found that high constituents of DAG increase the melting and crystallization temperature of MCO, lipase Novozym 435 gives the best yield of targeted nutrients (DAG, 1,3-DAG, LA-DAG), and the mixture of lipases, Lipozyme TL IM and Lipozyme RM IM, shows a synergistic effect in the synthetic process of DAG. Subsequently, MCO containing 65.4% DAG, 54.7% LA-DAG, and 47.6% 1,3-DAG content at optimal conditions (2% enzyme dosage, 4 h reaction time, 2.4:1 substrate molar ratio, 25.8% t-butanol as solvent, 60°C temperature) has been obtained and purified using silica column to obtain the final DAG oil containing 96.1% DAG, 64.7% 1,3-DAG, and 78.4% LA-DAG. High constituents of structured DAG oil rich in LA-1,3-DAG can be obtained by enzymatic esterification for industrial production.
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http://dx.doi.org/10.1111/1750-3841.16261DOI Listing
July 2022

Actinobacteria Community and Their Antibacterial and Cytotoxic Activity on the Weizhou and Xieyang Volcanic Islands in the Beibu Gulf of China.

Front Microbiol 2022 12;13:911408. Epub 2022 Jul 12.

Sea Area Use Dynamic Supervising and Managing Center of Fangchenggang City, Fangchenggang, China.

Weizhou Island and Xieyang Island are two large and young volcanic sea islands in the northern part of the South China Sea. In this study, high-throughput sequencing (HTS) of 16S rRNA genes was used to explore the diversity of Actinobacteria in the Weizhou and Xieyang Islands. Moreover, a traditional culture-dependent method was utilized to isolate Actinobacteria, and their antibacterial and cytotoxic activities were detected. The alpha diversity indices (ACE metric) of the overall bacterial communities for the larger island (Weizhou) were higher than those for the smaller island (Xieyang). A beta diversity analysis showed a more dispersive pattern of overall bacterial and actinobacterial communities on a larger island (Weizhou). At the order level, Frankiales, Propionibacteriales, Streptomycetales, Micrococcales, Pseudonocardiales, Micromonosporales, Glycomycetales, Corynebacteriales, and Streptosporangiales were the predominant Actinobacteria. A total of 22.7% of the OTUs shared 88%-95% similarity with some known groups. More interestingly, 15 OTUs formed a distinct and most predominant clade, and shared identities of less than 95% with any known families. This is the first report about this unknown group and their 16S rRNA sequences obtained from volcanic soils. A total of 268 actinobacterial strains were isolated by the culture-dependent method. Among them, 55 species were isolated, representing that 76.6% of the total and were the most abundant. Moreover, some rare Actinobacteria were isolated. These included spp., spp., spp., spp., spp., and spp. Among them, eight spp. exhibited antibacterial activity against . Only three strains inhibited the growth of . Four strains showed good activity against aquatic pathogenic bacterial strains of . The cytotoxicity assay results showed that 27 strains (10.07%) exhibited cytotoxic activity against HeLa and A549 cell lines. Many actinobacterial strains with cytotoxic activity were identified as rare Actinobacteria, which illustrated that volcanic islands are vast reservoirs for Actinobacteria with promising antibacterial and cytotoxic activity. This study may significantly improve our understanding of actinobacterial communities on volcanic islands. The isolated Actinobacteria showed promising prospects for future use.
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http://dx.doi.org/10.3389/fmicb.2022.911408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317746PMC
July 2022

Application Value of the Diagnosis during Early Carcinoma of Upper Digestive Tract Based on Optical Enhanced Endoscopic Technique.

Comput Math Methods Med 2022 19;2022:9587070. Epub 2022 Jul 19.

Department of Gastroenterology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou University, Zhengzhou, 450000 Henan Province, China.

Objective: The diagnostic value of optical enhanced endoscopy in early cancer of upper digestive tract was studied by comparing the disease accuracy, tumor type, invasion, and various surgical indicators between the two groups.

Methods: 188 patients with early upper gastrointestinal cancer treated in our hospital from January 2020 to February 2021 were selected as the research objects. The patients were randomly divided into the observation group and control group with 94 cases in each group.

Results: The accuracy of early detection of early carcinoma of upper digestive tract in the observation group was 94.68% and that in the control group was 76.60%. The accuracy of the observation group was significantly higher than that in the control group, with statistical significance ( < 0.05). In the observation group, 36 cases of early gastric cancer, 28 cases of early esophageal cancer, and 30 cases of early colorectal cancer were detected; 25 cases of early gastric cancer, 19 cases of early esophageal cancer, and 28 cases of early colorectal cancer were detected; 26 cases of early carcinoma of upper digestive tract infiltration were detected; and 68 cases were not detected, and the detection rate was 27.66%, which was higher than 9.57% in the control group, and the difference was statistically significant ( < 0.05). After different methods of treatment, no death occurred in all patients. Except for the operation time, the surgical indexes of the observation group were better than the control group, the difference was statistically significant ( < 0.05).

Conclusion: Optical enhanced endoscopic technique had obvious effect in the diagnosis of patients with early cancer of upper digestive tract, it was helpful to improve the clinical detection rate of early carcinoma of upper digestive tract and had certain diagnostic ability for the invasion depth of early cancer of high upper gastrointestinal tract, which was conducive to the detection of clinical invasion lesions and had high clinical promotion and application value.
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http://dx.doi.org/10.1155/2022/9587070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325562PMC
August 2022

Incidental Physical Pain Reduces Brain Activities Associated with Affective Social Feedback and Increases Aggression.

Soc Cogn Affect Neurosci 2022 Jul 27. Epub 2022 Jul 27.

Shanghai Key Laboratory of Mental Health and Psychological Crisis Intervention, Affiliated Mental Health Center (ECNU), School of Psychology and Cognitive Science, East China Normal University, Shanghai, China.

Physical pain may lead to aggressive behavior in a social context. However, it is unclear whether this is related to changes of social information processing. Thus, this study aimed to investigate the neural mechanisms underlying pain-induced aggression using functional magnetic resonance imaging. In the experiment, 59 healthy participants were recruited; 31 were treated with topical capsaicin cream (pain group), and 28 with hand cream (control group). Participants completed a social network aggression task, during which they underwent two phases: feedback processing; and attack exerting. Results revealed that participants in the pain group exhibited more aggression than those in the control group. During the feedback-processing phase, physical pain reduced brain activation in the right insula, left orbitofrontal cortex (OFC), and anterior cingulate cortex (ACC), which typically exhibited stronger activation in response to negative (and positive) versus neutral social feedback in the control group. However, during the attack-exerting phase, pain did not significantly alter activation of the dorsolateral prefrontal cortex (DLPFC). These findings suggest that pain increased aggression, while before that, it suppressed brain activities of the salience network involved in the process of salient social information and the value system associated with value representation of social events.
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http://dx.doi.org/10.1093/scan/nsac048DOI Listing
July 2022

Identification of Tumor Antigens and Immune Subtypes of Malignant Mesothelioma for mRNA Vaccine Development.

Vaccines (Basel) 2022 Jul 22;10(8). Epub 2022 Jul 22.

Clinical Cancer Center, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

Background: mRNA-based cancer vaccines have been considered a promising anticancer therapeutic approach against various cancers, yet their efficacy for malignant mesothelioma (MESO) is still not clear. The present study is designed to identify MESO antigens that have the potential for mRNA vaccine development, and to determine the immune subtypes for the selection of suitable patients.

Methods: A total of 87 MESO datasets were used for the retrieval of RNA sequencing and clinical data from The Cancer Genome Atlas (TCGA) databases. The possible antigens were identified by a survival and a genome analysis. The samples were divided into two immune subtypes by the application of a consensus clustering algorithm. The functional annotation was also carried out by using the DAVID program. Furthermore, the characterization of each immune subtype related to the immune microenvironment was integrated by an immunogenomic analysis. A protein-protein interaction network was established to categorize the hub genes.

Results: The five tumor antigens were identified in MESO. FAM134B, ALDH3A2, SAV1, and RORC were correlated with superior prognoses and the infiltration of antigen-presenting cells (APCs), while FN1 was associated with poor survival and the infiltration of APCs. Two immune subtypes were identified; TM2 exhibited significantly improved survival and was more likely to benefit from vaccination compared with TM1. TM1 was associated with a relatively quiet microenvironment, high tumor mutation burden, and enriched DNA damage repair pathways. The immune checkpoints and immunogenic cell death modulators were also differentially expressed between two subtypes. Finally, FN1 was identified to be the hub gene.

Conclusions: FAM134B, ALDH3A2, SAV1, RORC, and FN1 are considered as possible and effective mRNA anti-MESO antigens for the development of an mRNA vaccine, and TM2 patients are the most suitable for vaccination.
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http://dx.doi.org/10.3390/vaccines10081168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331978PMC
July 2022

Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial Pathogens.

J Fungi (Basel) 2022 Jun 30;8(7). Epub 2022 Jun 30.

Center for Yunnan Plateau Biological Resources Protection and Utilization, Yunnan Engineering Research Center of Fruit Wine, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing 655011, China.

Coffee endophytes have been studied for almost 74 years, and several studies have demonstrated coffee-endophytic fungi with antibacterial and antifungal potential for human and plant pathogens. In this study, we isolated and identified a total of 235 strains of endophytic fungi from coffee leaf tissues collected in four coffee plantations in Pu'er city, Yunnan province, China. Molecular identification was carried out using maximum likelihood phylogenetic analysis of nuclear ribosomal internal transcribed spacer (ITS1-5.8S rDNA-ITS2) sequences, while the colonization rate and the isolation frequency were also calculated. Two pathogenic fungi ( and ) and two pathogenic bacteria ( and subsp. ) were used for screening the antagonistic activities of 61 strains of coffee-endophytic fungi by a dual-culture test assay while maximum likelihood phylogenetic analysis confirmed their natural classification. This is the first study of coffee-leaf-endophytic fungal diversity in China, and the results revealed that coffee-endophytic fungi from this study belong to the Ascomycota, distributed among two classes, 10 orders, and 17 families. Concurrently, endophytic fungi isolates distributed in , , , and showed strong antagonistic activities against the pathogens. For the pathogens and , XCE-7 showed the best inhibitory effects with inhibition rates of 71.76% and 61.11%, respectively. For the pathogen , ME-9 showed the best inhibitory effect with a 74.67% inhibition rate, while PTE-7 and T5E-1-3 showed the best inhibitory effect with a rate of 60.42% against the pathogen subsp. . Overall, our study shows the diversity of coffee endophytes in four coffee-growing areas in Pu'er city, Yunnan province, China, and their potential use as biological control agents against two fungal and two bacterial pathogens.
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http://dx.doi.org/10.3390/jof8070698DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317674PMC
June 2022

Ionic Liquids: Emerging Antimicrobial Agents.

Pharm Res 2022 Jul 25. Epub 2022 Jul 25.

Key Laboratory of Smart Drug Delivery of MOE, School of Pharmacy, Fudan University, Shanghai, 201203, China.

Antimicrobial resistance has become a serious threat to global health. New antimicrobials are thus urgently needed. Ionic liquids (ILs), salts consisting of organic cations and anions with melting points less than 100°C, have been recently found to be promising in antimicrobial field as they may disrupt the bacterial wall and membrane and consequently lead to cell leakage and death. Different types of antimicrobial ILs are introduced in the review, including cationic, polymeric, and anionic ILs. Being the main type of the antimicrobial ILs, the review focuses on the structure and the antimicrobial mechanisms of cationic ILs. The quantitative structure-activity relationship (QSAR) models of the cationic ILs are also included. Increase in alkyl chain length and lipophilicity is beneficial to increase the antimicrobial effects of cationic ILs. Polymeric ILs are homopolymers of monomer ILs or copolymers of ILs and other monomers. They have great potential in the field of antibiotics as they provide stronger antimicrobial effects than the sum of the monomer ILs. Anionic ILs are composed of existing anionic antibiotics and organic cations, being capable to enhance the solubility and bioavailability of the original form. Nonetheless, the medical application of antimicrobial ILs is limited by the toxicity. The structural optimization aided by QSAR model and combination with existing antibiotics may provide a solution to this problem and expand the application range of ILs in antimicrobial field.
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http://dx.doi.org/10.1007/s11095-022-03336-5DOI Listing
July 2022

Enhanced inflammation and suppressed adaptive immunity in COVID-19 with prolonged RNA shedding.

Cell Discov 2022 Jul 25;8(1):70. Epub 2022 Jul 25.

Calibra Lab in DIAN Diagnostics, Key Laboratory of Digital Technology in Medical Diagnostics of Zhejiang Province, Hangzhou, Zhejiang, China.

Little is known regarding why a subset of COVID-19 patients exhibited prolonged positivity of SARS-CoV-2 infection. Here, we found that patients with long viral RNA course (LC) exhibited prolonged high-level IgG antibodies and higher regulatory T (Treg) cell counts compared to those with short viral RNA course (SC) in terms of viral load. Longitudinal proteomics and metabolomics analyses of the patient sera uncovered that prolonged viral RNA shedding was associated with inhibition of the liver X receptor/retinoid X receptor (LXR/RXR) pathway, substantial suppression of diverse metabolites, activation of the complement system, suppressed cell migration, and enhanced viral replication. Furthermore, a ten-molecule learning model was established which could potentially predict viral RNA shedding period. In summary, this study uncovered enhanced inflammation and suppressed adaptive immunity in COVID-19 patients with prolonged viral RNA shedding, and proposed a multi-omic classifier for viral RNA shedding prediction.
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http://dx.doi.org/10.1038/s41421-022-00441-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311354PMC
July 2022

The Application of Knowledge Distillation toward Fine-Grained Segmentation for Three-Vessel View of Fetal Heart Ultrasound Images.

Comput Intell Neurosci 2022 14;2022:1765550. Epub 2022 Jul 14.

Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Objectives: Measuring anatomical parameters in fetal heart ultrasound images is crucial for the diagnosis of congenital heart disease (CHD), which is highly dependent on the clinical experience of the sonographer. To address this challenge, we propose an automated segmentation method using the channel-wise knowledge distillation technique.

Methods: We design a teacher-student architecture to conduct channel-wise knowledge distillation. ROI-based cropped images and full-size images are used for the teacher and student models, respectively. It allows the student model to have both the fine-grained segmentation capability inherited from the teacher model and the ability to handle full-size test images. A total of 1,300 fetal heart ultrasound images of three-vessel view were collected and annotated by experienced doctors for training, validation, and testing.

Results: We use three evaluation protocols to quantitatively evaluate the segmentation accuracy: Intersection over Union (IoU), Pixel Accuracy (PA), and Dice coefficient (Dice). We achieved better results than related methods on all evaluation metrics. In comparison with DeepLabv3+, the proposed method gets more accurate segmentation boundaries and has performance gains of 1.8% on mean IoU (66.8% to 68.6%), 2.2% on mean PA (79.2% to 81.4%), and 1.2% on mean Dice (80.1% to 81.3%).

Conclusions: Our segmentation method could identify the anatomical structure in three-vessel view of fetal heart ultrasound images. Both quantitative and visual analyses show that the proposed method significantly outperforms the related methods in terms of segmentation results.
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http://dx.doi.org/10.1155/2022/1765550DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303103PMC
July 2022

Effect of a Functional Phospholipid Metabolome-Protein Association Pathway on the Mechanism of COVID-19 Disease Progression.

Int J Biol Sci 2022 11;18(12):4618-4628. Epub 2022 Jul 11.

Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, 510060, China.

This study aimed to explore the clinical practice of phospholipid metabolic pathways in COVID-19. In this study, 48 COVID-19 patients and 17 healthy controls were included. Patients were divided into mild (n=40) and severe (n=8) according to their severity. Phospholipid metabolites, TCA circulating metabolites, eicosanoid metabolites, and closely associated enzymes and transfer proteins were detected in the plasma of all individuals using metabolomics and proteomics assays, respectively. 30 of the 33 metabolites found differed significantly (<0.05) between patients and healthy controls (<0.05), with D-dimmer significantly correlated with all of the lysophospholipid metabolites (LysoPE, LysoPC, LysoPI and LPA). In particular, we found that phosphatidylinositol (PI) and phosphatidylcholine (PC) could identify patients from healthy controls (AUC 0.771 and 0.745, respectively) and that the severity of the patients could be determined (AUC 0.663 and 0.809, respectively). The last measurement before discharge also revealed significant changes in both PI and PC. For the first time, our study explores the significance of the phospholipid metabolic system in COVID-19 patients. Based on molecular pathway mechanisms, three important phospholipid pathways related to Ceramide-Malate acid (Cer-SM), Lysophospholipid (LPs), and membrane function were established. Clinical values discovered included the role of Cer in maintaining the inflammatory internal environment, the modulation of procoagulant LPA by upstream fibrinolytic metabolites, and the role of PI and PC in predicting disease aggravation.
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http://dx.doi.org/10.7150/ijbs.72450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305269PMC
July 2022

Pathogenic or Therapeutic: The Mediating Role of Gut Microbiota in Non-Communicable Diseases.

Front Cell Infect Microbiol 2022 7;12:906349. Epub 2022 Jul 7.

Department of Colorectal Surgery, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.

Noncommunicable diseases (NCDs) lead to 41 million deaths every year and account for 71% of all deaths worldwide. Increasing evidence indicates that gut microbiota disorders are closely linked to the occurrence and development of diseases. The gut microbiota, as a potential transmission medium, could play a key role in the transmission and treatment of diseases. The gut microbiota makes noncommunicable diseases communicable. New methods of the prevention and treatment of these diseases could be further explored through the gut microbiota.
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http://dx.doi.org/10.3389/fcimb.2022.906349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301375PMC
July 2022

Tumor vessel normalization and immunotherapy in gastric cancer.

Ther Adv Med Oncol 2022 18;14:17588359221110176. Epub 2022 Jul 18.

Lung Cancer Center, West China Hospital of Sichuan University, No. 37, Guo Xue Xiang, Wuhou District, Chengdu, Sichuan 610041, People's Republic of China.

Gastric cancer (GC) is a common malignant tumor, and patients with GC have a low survival rate due to limited effective treatment methods. Angiogenesis and immune evasion are two key processes in GC progression, and they act synergistically to promote tumor progression. Tumor vascular normalization has been shown to improve the efficacy of cancer immunotherapy, which in turn may be improved through enhanced immune stimulation. Therefore, it may be interesting to identify synergies between immunomodulatory agents and anti-angiogenic therapies in GC. This strategy aims to normalize the tumor microenvironment through the action of the anti-vascular endothelial growth factor while stimulating the immune response through immunotherapy and prolonging the survival of GC patients.
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http://dx.doi.org/10.1177/17588359221110176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297465PMC
July 2022

Mutual regulation of noncoding RNAs and RNA modifications in psychopathology: Potential therapeutic targets for psychiatric disorders?

Pharmacol Ther 2022 Jul 21;237:108254. Epub 2022 Jul 21.

Department of Pharmacology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine, Southeast University, Nanjing, China; Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China. Electronic address:

The human brain is specifically enriched for multiple classes of noncoding RNAs (ncRNAs) and for particular RNA modifications, both of which are increasingly recognized to contribute to the etiology and pathophysiology of psychiatric disorders. Here, we summarize the rapidly developing areas of basic research in brain-specific ncRNA biology and the functional and pathological consequences of different RNA modifications. In particular, multiple studies have identified mutual regulation between ncRNAs and RNA modifications. Specifically, RNA methylation of ncRNAs can regulate their cleavage and maturation, intracellular transport, stability, and ultimately their degradation. Alternatively, ncRNAs can affect RNA modifications by up- or down-regulating target protein expression or by altering their subcellular distribution, among several other effects. Growing clinical and preclinical research attention is currently being focused on exploring the pathological impacts and highly diverse molecular regulatory mechanisms of ncRNAs and RNA modifications in psychiatric disorders. Here, we review recent findings surrounding the mutual regulation between ncRNAs and RNA modifications in brain psychopathology. We also discuss advances in basic discovery and clinical translation or therapeutic potential of targeting ncRNAs and/or RNA modification regulators in psychiatric disorders.
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http://dx.doi.org/10.1016/j.pharmthera.2022.108254DOI Listing
July 2022

The spatial differences of the synergy between CO and air pollutant emissions in China's 296 cities.

Sci Total Environ 2022 Jul 19;846:157323. Epub 2022 Jul 19.

School of Economics and Management, China University of Geosciences, Beijing 100083, China; Key Laboratory of Carrying Capacity Assessment for Resource and Environment, Ministry of Natural Resources of the People's Republic of China, Beijing 100083, China.

The emissions reduction of CO and air pollutants are the main task in China. The two have the same roots and they interact with each other. However, CO and air pollutants are quite different in space, so it is of great practical significance to explore the spatial differences of their synergy. As PM and O are more concerned at present, thus, this paper examined the decoupling of CO, PM and O from GDP in China's 296 cities using the latest available data from 2015 to 2016. And the spatial differences of synergy among CO, PM and O were quantitatively analyzed by using spatial autocorrelation analysis and geographically weighted regression model. The results showed that: (1) The cities achieving the three synergy emissions reduction were mainly in the southeast of China. (2) Only 26 cities had achieved the strong decoupling of CO, PM and O from GDP. (3) The synergy characteristics between CO and PM, CO and O were different. This paper put forward the policies according to the conclusions.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157323DOI Listing
July 2022

Isolation and genomic characterization of a novel Autographiviridae bacteriophage IME184 with lytic activity against Klebsiella pneumoniae.

Virus Res 2022 Jul 19;319:198873. Epub 2022 Jul 19.

College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 10029, China. Electronic address:

Klebsiella pneumoniae, a multidrug resistant bacterium that causes nosocomial infections including septicemia, pneumonia etc. Bacteriophages are potential antimicrobial agents for the treatment of antibiotic resistant bacteria. In this study, a novel bacteriophage IME184, was isolated from hospital sewage against clinical multi-drug resistant Klebsiella pneumoniae. Transmission electron microscopy and genomic characterization exhibited this phage belongs to the Molineuxvirinae genus, Autographiviridae family. Phage IME184 possessed a double-stranded DNA genome composed of 44,598 bp with a GC content of 50.3%. The phage genome encodes 57 open reading frames, out of 26 are hypothetical proteins while 31 had assigned putative functions. No tRNA, virulence related or antibiotic resistance genes were found in phage genome. Comparative genomic analysis showed that phage IME184 has 94% similarity with genomic sequence of Klebsiella phage K1-ULIP33 (MK380014.1). Multiplicity of infection, one step growth curve and host range of phage were also measured. According to findings, Phage IME184 is a promising biological agent that infects Klebsiella pneumoniae and can be used in future phage therapies.
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http://dx.doi.org/10.1016/j.virusres.2022.198873DOI Listing
July 2022
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