Publications by authors named "Lu Han"

1,045 Publications

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Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems.

Nat Protoc 2022 Aug 17. Epub 2022 Aug 17.

Center for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children's Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine, Cincinnati, OH, USA.

Development of visceral organs such as the esophagus, lung, liver and stomach are coordinated by reciprocal signaling interactions between the endoderm and adjacent mesoderm cells in the fetal foregut. Although the recent successes in recapitulating developmental signaling in vitro has enabled the differentiation of human pluripotent stem cells (hPSCs) into various types of organ-specific endodermal epithelium, the generation of organ-specific mesenchyme has received much less attention. This is a major limitation in ongoing efforts to engineer complex human tissue. Here, we describe a protocol to differentiate hPSCs into different types of organ-specific mesoderm, leveraging signaling networks and molecular markers elucidated from single-cell transcriptomics of mouse foregut organogenesis. Building on established methods, hPSC-derived lateral plate mesoderm treated with either retinoic acid (RA) or RA together with a Hedgehog (HH) agonist generates posterior or anterior foregut splanchnic mesoderm, respectively, after 4-d cultures. These are directed into organ-specific mesenchyme lineages by the combinatorial activation or inhibition of WNT, BMP, RA or HH pathways from days 4 to 7 in cultures. By day 7, the cultures are enriched for different types of mesoderm with distinct molecular signatures: 60-90% pure liver septum transversum/mesothelium-like, 70-80% pure liver-like fibroblasts and populations of ~35% respiratory-like mesoderm, gastric-like mesoderm or esophageal-like mesoderm. This protocol can be performed by anyone with moderate experience differentiating hPSCs, provides a novel platform to study human mesoderm development and can be used to engineer more complex foregut tissue for disease modeling and regenerative medicine.
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http://dx.doi.org/10.1038/s41596-022-00733-3DOI Listing
August 2022

Single-walled carbon nanotube gutter layer supported ultrathin zwitterionic microporous polymer membrane for high-performance lithium-sulfur battery.

J Colloid Interface Sci 2022 Aug 8;628(Pt A):1012-1022. Epub 2022 Aug 8.

National-Local Joint Engineering Laboratory for Energy Conservation in Chemical Process Integration and Resources Utilization, School of Chemical Engineering and Technology, Hebei University of Technology, No. 8, Guangrong Road, Tianjin 300130, China; Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada.

Development of efficient lithium-sulfur (Li-S) battery requires the need to develop an appropriate functional separator that allows strong facilitation and transport of lithium ions together with limited passage of polysulfides. In this work, a multifunctional separator (TB-BAA/SWCNT/PP) is developed through spin coating of a novel zwitterionic microporous polymer (TB-BAA) on the gutter layer constructed from single-walled carbon nanotubes (SWCNT), where commercially available polypropylene (PP) separator is used to act as the mechanical support. SWCNT in this study serves as the first modification layer to decrease the size of the macropores in the PP separator, while the ultrathin TB-BAA top barrier layer with the presence of zwitterionic side chains allows the creation of confined ionic channels with both lithiophilic and sulfophilic groups. Due to the presence of available chemical interactions with lithium polysulfides, selective ion transport can be foreseen through such separator. In this regard, shuttle effect that is frequently encountered in Li-S battery can be suppressed effectively via implementing the as-obtained functional separator, resulting in the creation of credible and stable sulfur electrochemistry. The TB-BAA/SWCNT/PP-based Li-S battery has been investigated to possess high cycling ability (capacity fading per cycle of 0.055% over 500 cycles at 1 C) together with decent rate capability (736.6 mAh g at 3 C). In addition, a high areal capacity retention of 5.03 mAh cm after 50 cycles can be also obtained under raised sulfur loading (5.4 mg cm).
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http://dx.doi.org/10.1016/j.jcis.2022.08.025DOI Listing
August 2022

Hyperthermia based individual recombinant vaccine enhances lymph nodes drainage for antitumor immunity.

Acta Pharm Sin B 2022 Aug 26;12(8):3398-3409. Epub 2022 Feb 26.

Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang 471009, China.

The continuing challenges that limit effectiveness of tumor therapeutic vaccines were high heterogeneity of tumor immunogenicity, low bioactivity of antigens, as well as insufficient lymph nodes (LNs) drainage of antigens and adjuvants. Transportation of neoantigens and adjuvants to LNs may be an effective approach to solve the abovementioned problems. Therefore, an FA-TSL/AuNCs/SV nanoplatform was constructed by integrating simvastatin (SV) adjuvant loaded Au nanocages (AuNCs) as cores (AuNCs/SV) and folic acid modified thermal-sensitive liposomes (FA-TSL) as shells to enhance antitumor immunity. After accumulation in tumor guided by FA, AuNCs mediated photothermal therapy (PTT) induced the release of tumor-derived protein antigens (TDPAs) and the shedding of FA-TSL. Exposed AuNCs/SV soon captured TDPAs to form recombinant vaccine (AuNCs/SV/TDPAs). Subsequently, AuNCs/SV/TDPAs could efficiently transport to draining LNs owing to the hyperthermia induced vasodilation effect and small particle size, achieving co-delivery of antigens and adjuvant for initiation of specific T cell response. In melanoma bearing mice, FA-TSL/AuNCs/SV and laser irradiation effectively ablated primary tumor, against metastatic tumors and induced immunological memory. This approach served a hyperthermia enhanced platform drainage to enable robust personalized cancer vaccination.
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http://dx.doi.org/10.1016/j.apsb.2022.02.026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366229PMC
August 2022

Risk Factors for Mortality and Outcomes in Hematological Malignancy Patients with Carbapenem-Resistant Bloodstream Infections.

Infect Drug Resist 2022 4;15:4241-4251. Epub 2022 Aug 4.

Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.

Background: This study aimed to identify risk factors for mortality and outcomes in hematological malignancy (HM) patients with bloodstream infection (BSI) caused by carbapenem-resistant (CRKP).

Methods: A retrospective study was conducted at a tertiary teaching hospital in Henan Province, China, between January 2018 and December 2021. All BSIs caused by CRKP in hospitalized HM patients were identified. Data on patient demographics, disease, laboratory tests, treatment regimens, outcomes of infection, and the antimicrobial susceptibility of each isolate were collected from medical records.

Results: A total of 129 patients with CRKP BSI were included in the study, and the 28-day mortality rate was 80.6% (104/129). In Cox analysis an absolute neutrophil count < 500 at discharge (hazard ratio [HR] 6.386, 95% confidence interval [CI] 3.074-13.266, < 0.001), intensive care unit admission (HR 1.834, 95% CI 1.065-3.157, = 0.029), and higher Pitt bacteremia score (HR 1.185, 95% CI 1.118-1.255, < 0.001) were independent risk factors associated with 28-day mortality. Survival curve analysis indicated that compared with ceftazidime-avibactam-based therapy, both polymyxin b (HR 8.175, 95% CI 1.099-60.804, = 0.040) and tigecycline (HR 14.527, 95% CI 2.000-105.541, =0.008) were associated with a higher risk of mortality.

Conclusion: In HM patients CRKP BSI resulted in high mortality. Intensive care unit admission, higher Pitt bacteremia score, and absolute neutrophil count < 500 at discharge were independently associated with higher mortality. Early initiation of new agents such as ceftazidime-avibactam may improve outcomes.
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http://dx.doi.org/10.2147/IDR.S374904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359710PMC
August 2022

Health service planning to assess the expected impact of centralising specialist cancer services on travel times, equity, and outcomes: a national population-based modelling study.

Lancet Oncol 2022 Aug 2. Epub 2022 Aug 2.

Department of Health Services Research and Policy, London School of Hygiene & Tropical Medicine, London, UK.

Background: Centralisation of specialist cancer services is occurring in many countries, often without evaluating the potential impact before implementation. We developed a health service planning model that can estimate the expected impacts of different centralisation scenarios on travel time, equity in access to services, patient outcomes, and hospital workload, using rectal cancer surgery as an example.

Methods: For this population-based modelling study, we used routinely collected individual patient-level data from the National Cancer Registration and Analysis Service (NCRAS) and linked to the NHS Hospital Episode Statistics (HES) database for 11 888 patients who had been diagnosed with rectal cancer between April 1, 2016, and Dec 31, 2018, and who subsequently underwent a major rectal cancer resection in 163 National Health Service (NHS) hospitals providing rectal cancer surgery in England. Five centralisation scenarios were considered: closure of lower-volume centres (scenario A); closure of non-comprehensive cancer centres (scenario B); closure of centres with a net loss of patients to other centres (scenario C); closure of centres meeting all three criteria in scenarios A, B, and C (scenario D); and closure of centres with high readmission rates (scenario E). We used conditional logistic regression to predict probabilities of affected patients moving to each of the remaining centres and the expected changes in travel time, multilevel logistic regression to predict 30-day emergency readmission rates, and linear regression to analyse associations between the expected extra travel time for patients whose centre is closed and five patient characteristics, including age, sex, socioeconomic deprivation, comorbidity, and rurality of the patients' residential areas (rural, urban [non-London], or London). We also quantified additional workload, defined as the number of extra patients reallocated to remaining centres.

Findings: Of the 11 888 patients, 4130 (34·7%) were women, 5249 (44·2%) were aged 70 years and older, and 5005 (42·1%) had at least one comorbidity. Scenario A resulted in closures of 43 (26%) of the 163 rectal cancer surgery centres, affecting 1599 (13·5%) patients; scenario B resulted in closures of 112 (69%) centres, affecting 7029 (59·1%) patients; scenario C resulted in closures of 56 (34%) centres, affecting 3142 (26·4%) patients; scenario D resulted in closures of 24 (15%) centres, affecting 874 (7·4%) patients; and scenario E resulted in closures of 16 (10%) centres, affecting 1000 (8·4%) patients. For each scenario, there was at least a two-times increase in predicted travel time for re-allocated patients with a mean increase in travel time of 23 min; however, the extra travel time did not disproportionately affect vulnerable patient groups. All scenarios resulted in significant reductions in 30-day readmission rates (range 4-48%). Three hospitals in scenario A, 41 hospitals in in scenario B, 13 hospitals in scenario C, no hospitals in scenario D, and two hospitals in scenario E had to manage at least 20 extra patients annually.

Interpretation: This health service planning model can be used to to guide complex decisions about the closure of centres and inform mitigation strategies. The approach could be applied across different country or regional health-care systems for patients with cancer and other complex health conditons.

Funding: National Institute for Health Research.
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http://dx.doi.org/10.1016/S1470-2045(22)00398-9DOI Listing
August 2022

Urinary Aromatic Amino Acid Metabolites Associated With Postoperative Emergence Agitation in Paediatric Patients After General Anaesthesia: Urine Metabolomics Study.

Front Pharmacol 2022 19;13:932776. Epub 2022 Jul 19.

Department of Pharmacy, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China.

Emergence agitation (EA) is very common in paediatric patients during recovery from general anaesthesia, but underlying mechanisms remain unknown. This prospective study was designed to profile preoperative urine metabolites and identify potential biomarkers that can predict the occurrence of EA. A total of 224 patients were screened for recruitment; of those, preoperative morning urine samples from 33 paediatric patients with EA and 33 non-EA gender- and age-matched patients after being given sevoflurane general anaesthesia were analysed by ultra-high-performance liquid chromatography (UHPLC) coupled with a Q Exactive Plus mass spectrometer. Univariate analysis and orthogonal projection to latent structures squares-discriminant analysis (OPLS-DA) were used to analyse these metabolites. The least absolute shrinkage and selection operator (LASSO) regression was used to identify predictive variables. The predictive model was evaluated through the receiver operating characteristic (ROC) analysis and then further assessed with 10-fold cross-validation. Seventy-seven patients completed the study, of which 33 (42.9%) patients developed EA. EA and non-EA patients had many differences in preoperative urine metabolic profiling. Sixteen metabolites including nine aromatic amino acid metabolites, acylcarnitines, pyridoxamine, porphobilinogen, 7-methylxanthine, and 5'-methylthioadenosine were found associated with an increased risk of EA, and they all exhibited higher levels in the EA group than in the non-EA group. The main metabolic pathways involved in these metabolic changes included phenylalanine, tyrosine and tryptophan metabolisms. Among these potential biomarkers, L-tyrosine had the best predictive value with an odds ratio (OR) (95% CI) of 5.27 (2.20-12.63) and the AUC value of 0.81 (0.70-0.91) and was robust with internal 10-fold cross-validation. Urinary aromatic amino acid metabolites are closely associated with EA in paediatric patients, and further validation with larger cohorts and mechanistic studies is needed. clinicaltrials.gov, identifier NCT04807998.
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http://dx.doi.org/10.3389/fphar.2022.932776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343964PMC
July 2022

Zwitterionic covalent organic framework as a multifunctional sulfur host toward durable lithium-sulfur batteries.

J Colloid Interface Sci 2022 Jul 22;628(Pt A):144-153. Epub 2022 Jul 22.

National-Local Joint Engineering Laboratory for Energy Conservation in Chemical Process Integration and Resources Utilization, School of Chemical Engineering and Technology, Hebei University of Technology, No. 8, Guangrong Road, Tianjin 300130, China. Electronic address:

The shuttle effect and slow redox kinetics of sulfur cathode are the most significant technical challenges to the practical application of lithium-sulfur (Li-S) battery. Herein, a novel zwitterionic covalent organic framework (ZW-COF) wrapped onto carbon nanotubes (CNTs), labeled as [email protected], is developed by a reversible condensation reaction of 1,3,5-benzenetricarboxaldehyde (BTA) and 3,8-diamino-6-phenylphenanthridine (DPPD) with CNTs as a template and a subsequently-one-step post-synthetic grafting reaction with 1,3-propanesultone. The experimental results showed that, after loading active material sulfur, zwitterionic [email protected] can effectively suppress the shuttle effect of the soluble lithium polysulfides (LiPSs) in Li-S batteries, and exhibits better cycling behavior than the as-developed neutral [email protected] Specifically, the as-obtained [email protected] based sulfur cathode can maintain a discharge capacity of 944 mAh/g after 100 cycles, while that of [email protected] based sulfur cathode drops to (665 mAh/g) after 100 cycles. Moreover, the [email protected] based sulfur cathode delivers an attractive prolonged cycling behavior with a low capacity decay rate of 0.046 % per cycle at 1 C. This work sheds new light on rational selection and design of functionalized COFs based sulfur cathode in the Li-S battery.
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http://dx.doi.org/10.1016/j.jcis.2022.07.123DOI Listing
July 2022

Combination of Low-Dose Gemcitabine and PD-1 Inhibitors for Treatment in Patients With Advanced Malignancies.

Front Immunol 2022 13;13:882172. Epub 2022 Jul 13.

Department of Immunotherapy, the Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, China.

Purpose: This study determined the efficacy of low-dose gemcitabine combined with programmed death-1 (PD-1) inhibitors for treating multiple malignancies, providing a cost-effective and safe treatment option.

Study Design: This study included 61 patients with advanced solid tumors treated with low-dose gemcitabine combined with PD-1 inhibitors at the Henan Cancer Hospital between January 2018 and February 2022. We retrospectively reviewed medical records to evaluate several clinical factors, including progression-free survival (PFS), overall survival (OS), adverse effects (AEs), and objective response to treatment.

Results: Sixty-one patients received treatment with low-dose gemcitabine combined with PD-1 inhibitors. The objective response rate (ORR) was 29.5% and the disease control rate (DCR) was 62.3%. The median PFS was 4.3 months (95% confidence interval, 2.3 to 6.3 months) and the median OS was 15.0 months (95% confidence interval, 8.8 to 21.2 months). Hematological toxicity, mainly leukopenia or thrombocytopenia, was the most common AE, with any-grade and grade 3/4 hematological toxicity reported in 60.7 and 13.1% of patients, respectively.

Conclusions: Low-dose gemcitabine combined with PD-1 inhibitors may offer a novel treatment option for patients with advanced malignancies.
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http://dx.doi.org/10.3389/fimmu.2022.882172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328170PMC
July 2022

BDNF Alleviates Microglial Inhibition and Stereotypic Behaviors in a Mouse Model of Obsessive-Compulsive Disorder.

Front Mol Neurosci 2022 12;15:926572. Epub 2022 Jul 12.

Department of Psychology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, China.

Obsessive-compulsive disorder (OCD) is a severe mental illness characterized by obsessions and compulsions. However, its underlying mechanisms remain to be elucidated. Recent studies have suggested that neuroimmune dysregulation is involved in the pathogenesis of OCD. To investigate the role of microglia in this disorder, we established a pharmacological mouse model by using the serotonin (5-HT) 1A/1B receptor agonist RU24969 to mimic monoamine dysregulation in OCD, and we examined the morphological and functional alterations of microglia in this model. We found that RU24969 treatment led to compulsive circling behavior in mice. Strikingly, we found that the density and mobility of microglia in the prelimbic cortex were much lower in RU24969-treated mice than in control mice. Moreover, the expression of cytokines and chemokines, including BDNF, IL-1β, IL-6, TNFα, CD80, CD86, MHC-I, and MHC-II, also decreased in RU24969-treated mice. Importantly, we found that injection of BDNF or induction of BDNF expression by trehalose completely reversed microglial dysfunction and reduced stereotypic behavior. These results indicate that microglial dysfunction is closely related to stereotypic behaviors in our mouse model of OCD and that BDNF could be an effective treatment for stereotypic behaviors.
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http://dx.doi.org/10.3389/fnmol.2022.926572DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325681PMC
July 2022

Association of hepatitis B virus DNA levels with overall survival for advanced hepatitis B virus-related hepatocellular carcinoma under immune checkpoint inhibitor therapy.

Cancer Immunol Immunother 2022 Jul 30. Epub 2022 Jul 30.

Department of Immunotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, China.

Background: High hepatitis B virus (HBV) DNA level is an independent risk factor for postoperative HBV-associated liver cancer recurrence. We sought to examine whether HBV DNA level and antiviral therapy are associated with survival outcomes in patients with advanced hepatocellular carcinoma (HCC) treated with anti-programmed cell death protein 1 (PD-1)based immunotherapy.

Methods: This single-institution retrospective analysis included 217 patients with advanced HBV-related HCC treated from 1 June 2018, through 30 December 2020. Baseline information was compared between patients with low and high HBV DNA levels. Overall survival (OS) and progression-free survival (PFS) were compared, and univariate and multivariate analyses were applied to identify potential risk factors for oncologic outcomes.

Results: The 217 patients included in the analysis had a median survival time of 20.6 months. Of these HBV-associated HCC patients, 165 had known baseline HBV DNA levels. Baseline HBV DNA level was not significantly associated with OS (P = 0.59) or PFS (P = 0.098). Compared to patients who did not receive antiviral therapy, patients who received antiviral therapy had significantly better OS (20.6 vs 11.1 months, P = 0.020), regardless of HBV DNA levels. Moreover, antiviral status (adjusted HR = 0.24, 95% CI 0.094-0.63, P = 0.004) was an independent protective factor for OS in a multivariate analysis of patients with HBV-related HCC.

Conclusions: HBV viral load does not compromise the clinical outcome of patients with HBV-related HCC treated with anti-PD-1-based immunotherapy. The use of antiviral therapy significantly improves survival time of HBV-related HCC patients.
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http://dx.doi.org/10.1007/s00262-022-03254-wDOI Listing
July 2022

Recent Progress on Graphene Flexible Photodetectors.

Materials (Basel) 2022 Jul 11;15(14). Epub 2022 Jul 11.

Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.

In recent years, optoelectronics and related industries have developed rapidly. As typical optoelectronics devices, photodetectors (PDs) are widely applied in various fields. The functional materials in traditional PDs exhibit high hardness, and the performance of these rigid detectors is thus greatly reduced upon their stretching or bending. Therefore, the development of new flexible PDs with bendable and foldable functions is of great significance and has much interest in wearable, implantable optoelectronic devices. Graphene with excellent electrical and optical performance constructed on various flexible and rigid substrates has great potential in PDs. In this review, recent research progress on graphene-based flexible PDs is outlined. The research states of graphene conductive films are summarized, focusing on PDs based on single-component graphene and mixed-structure graphene, with a systematic analysis of their optical and mechanical performance, and the techniques for optimizing the PDs are also discussed. Finally, a summary of the current applications of graphene flexible PDs and perspectives is provided, and the remaining challenges are discussed.
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http://dx.doi.org/10.3390/ma15144820DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318373PMC
July 2022

[Exploration on mechanism of Polygalae Radix and Acori Tatarinowii Rhizoma in treating Alzheimer's disease based on network pharmacology and experimental verification].

Zhongguo Zhong Yao Za Zhi 2022 Jun;47(12):3348-3360

Institute of Clinical Pharmacology/Science and Technology Innovation Center, Guangzhou University of Chinese Medicine Guangzhou 510405, China.

This study aimed to explore the action targets and mechanisms of Polygala tenuifolia and Acorus tatarinowii in treating Alzheimer's disease(AD) based on network pharmacology, molecular docking, and animal tests. The AD-related targets were collec-ted from GeneCard and the main active ingredients and targets of P. tenuifolia and A. tatarinowii from the TCMSP. Cytoscape was applied to construct the "Chinese herb-active ingredient-target-disease" network, followed by the construction of protein-protein interaction(PPI) network using STRING. GO biological function and KEGG pathway enrichment analysis was performed by DAVID and Metascape. The main active components of P. tenuifolia and A. tatarinowii and their potential core targets were docking using AutoDock Vina. The effects of P. tenuifolia and A. tatarinowii on the cognitive function were verified in mice with scopolamine(SCOP)-induced cognitive impairment. A total of seven active ingredients including kaempferol, onjixanthone Ⅰ, and marmesin and 56 potential targets of P. tenuifolia and A. tatarinowii were screened out, with the core targets covering AKT1, PTGS2, TNF, and NF-κB inflammation pathway mainly involved. The results of molecular docking also showed that the main active components of P. tenuifolia and A. tatarinowii stably bond to the core targets predicted by network pharmacology. The new object recognition experiment suggested that P. tenuifolia and A. tatarinowii improved the learning and memory abilities of mice after SCOP induction. As revealed by pathological section observation and relevant kit assay, P. tenuifolia and A. tatarinowii reduced the damage of central cholinergic neurons and enhanced the antioxidant ability of SCOP-induced mice. Western blot confirmed that P. tenuifolia and A. tatarinowii down-regulated the protein expression levels of TLR4, NF-κB, and related inflammatory factors(TNF-α, IL-1β, and IL-6). All these have suggested that P. tenuifolia and A. tatarinowii inhibits AD via multiple components, multiple targets, and multiple pathways, which has provided an experimental basis for the clinical application of P. tenuifolia and A. tatarinowii for the treatment of AD.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20211216.707DOI Listing
June 2022

Synthesis and application of a novel polyurethane nanoemulsion bearing coumarin derivative as a "turn-on" fluorescence sensor toward Hg.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Nov 9;281:121612. Epub 2022 Jul 9.

Department of Chemical Engineering and Key Laboratory of Advanced Materials of Ministry of Education of China, Tsinghua University, Beijing 100084, China. Electronic address:

A novel polyurethane (PU-co-HCCA) nanoemulsion bearing coumarin derivative (HCCA) was synthesized as a "turn-on" fluorescent probe and used to modify filter paper, and its sensing properties were investigated. Results showed that PU-co-HCCA nanoemulsion exhibited high selectivity and excellent sensitivity toward Hg over other metal ions, and possessed excellent fluorescence quantum yields of 0.976, ppb-levels detection limits of 1.61 ppb and large Stokes shifts of 101 nm. Meanwhile, as an application example of as-prepared PU-co-HCCA nanoemulsion, a Hg test paper was prepared by modifying filter paper with PU-co-HCCA nanoemulsion, and results indicated that the test paper is portable and convenient and has a wide working pH range. We believe that the PU-co-HCCA nanoemulsion and the modified filter paper can provide a new design principle for the application of fluorescence sensors for metal ions including Hg.
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http://dx.doi.org/10.1016/j.saa.2022.121612DOI Listing
November 2022

Expression of Serum PSA, Nesfatin-1, and AMH in Patients with Polycystic Ovary Syndrome.

Cell Mol Biol (Noisy-le-grand) 2022 Feb 4;67(5):57-63. Epub 2022 Feb 4.

Department of Gynecology, The Fourth Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, China.

Insulin resistance and hyperandrogenism are the leading causes of polycystic ovary syndrome (PCOS). Therefore, it has great significance to study the expression levels of PSA, nesfatin-1, and AMH. To provide some reference for clinical diagnosis and treatment of polycystic ovary syndrome (PCOS), the expression levels of PSA, nesfatin-1, and AMH in serum of patients with polycystic ovary syndrome (PCOS) were investigated. The experimental group consisted of 200 patients with polycystic ovary syndrome treated in Shanghai Huashan Hospital from July 2018 to July 2019. The control group consisted of 150 healthy women without pregnancy. The PSA, nesfatin-1, and AMH levels in serum were detected by chemiluminescence immunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA). The serum levels of prostate-specific antigen (PSA) and anti-Mullerian hormone (AMH) were 16.53 ± 0.67pg/ml and 10.75 ± 4.02pg/ml in the experimental group (PCOS patients), which were significantly higher than those in the control group (3.27 ± 0.43pg/ml and 5.18 ± 1.84pg/ml, respectively), while the inhibitive factors in the experimental group (1.89 ± 0.99mg/ml) were significantly higher than those in the control group (1.10 ± 0.97mg/ml). There was no significant difference in nesfatin-1. The levels of PSA and nesfatin-1, nesfatin-1, and AMH and the levels of PSA and AMH in patients with polycystic ovary syndrome were positively correlated, and the differences were statistically significant. The levels of PSA, nesfatin-1, and AMH in patients with polycystic ovary syndrome of different ages were different, and the differences were significant and negatively correlated with the age increasing. PSA, nesfatin-1, and AMH levels in patients with polycystic ovary syndrome were significantly different from those in control nonpregnant women. There was a certain correlation between the levels of PSA, nesfatin-1, and AMH, and age. The results have specific clinical reference significance for the diagnosis and treatment of patients with polycystic ovary syndrome.
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http://dx.doi.org/10.14715/cmb/2021.67.5.8DOI Listing
February 2022

Association between Vitamin D Deficiency and Levels of Renin and Angiotensin in Essential Hypertension.

Int J Clin Pract 2022 10;2022:8975396. Epub 2022 Jun 10.

Department of Hypertension, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.

Objective: The present study aims to investigate the relationship between vitamin D deficiency and renin-angiotensin-aldosterone levels in patients with essential hypertension.

Methods: The present study observed two groups of patients from Urumqi, Xinjiang, China, from April 2017 to March 2018. There were two subject groups: the hypertension group (80 patients with essential hypertension selected by random cluster sampling) and the control group (76 healthy adults). The 25-hydroxyvitamin D (25(OH)D or vitamin D) levels were measured through electrolytes; fasting blood glucose, blood lipids, and other biochemical indicators were detected using immune chemiluminescence; and plasma renin activity and angiotensin II concentrations were detected with radio-immunity.

Results: Comparison between the hypertension group and control group showed statistically significant differences in the systolic pressure and levels of 25(OH)D, renin, and triglycerides ( < 0.05). The correlation analysis showed that 25(OH)D was negatively correlated with renin ( = -0.185; =0.021) and positively correlated with systolic pressure ( = -0.105; =0.035). There were no statistically significant differences in diastolic pressure, fasting blood glucose, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, and triglycerides between the two groups.

Conclusions: The results of the present study show that vitamin D deficiency is common in Urumqi, Xinjiang, China and vitamin D levels are negatively correlated with renin levels. Vitamin D plays an important role in regulating blood pressure by affecting renin levels through the renin-angiotensin-aldosterone system.
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http://dx.doi.org/10.1155/2022/8975396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205726PMC
July 2022

Degradation of phenanthrene by consortium 5H under hypersaline conditions.

Environ Pollut 2022 Sep 6;308:119730. Epub 2022 Jul 6.

Miami College, Henan University, Kaifeng, 475000, Henan, China. Electronic address:

PAHs have been widely detected to accumulate in saline and hypersaline environments. Moderately halophilic microbes are considered the most suitable player for the elimination of PAHs in such environments. In this study, consortium 5H was enriched under 5% salinity and completely degraded phenanthrene in 5 days. By high-throughput sequencing, consortium 5H was identified as being mainly composed of Methylophaga, Marinobacter and Thalassospira. Combined with the investigation of intermediates and enzymatic activities, the degradation pathway of consortium 5H on phenanthrene was proposed. Consortium 5H was identified as having the ability to tolerate a wide range of salinities (1%-10%) and initial PAH concentrations (50 mg/L to 400 mg/L). It was also able to function under neutral to weak alkaline conditions (pH from 6 to 9) and the phytotoxicity of the produced intermediates showed no significant difference with distilled water. Furthermore, the metagenome of consortium 5H was measured and analyzed, which showed a great abundance of catabolic genes contained in consortium 5H. This study expanded the knowledge of PAH-degradation under hypersaline environments and consortium 5H was proposed to have good potential for the elimination of PAH pollution in saline/hypersaline environments.
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http://dx.doi.org/10.1016/j.envpol.2022.119730DOI Listing
September 2022

Triblock Copolymer Compatibilizers for Enhancing the Mechanical Properties of a Renewable Bio-Polymer.

Polymers (Basel) 2022 Jul 4;14(13). Epub 2022 Jul 4.

State Key Laboratory of Automotive Simulation and Control, College of Automotive Engineering, Jilin University, Changchun 130022, China.

Poly(lactic acid) (PLA) is an emerging plastic that has insufficient properties (e.g., it is too brittle) for widespread commercial use. Previous research results have shown that the strength and toughness of basalt fiber reinforced PLA composites (PLA/BF) still need to be improved. To address this limitation, this study aimed to obtain an effective compatibilizer for PLA/BF. Melt-blending of poly(butylene adipate-co-terephthalate) (PBAT) with PLA in the presence of 4,4'-methylene diphenyl diisocyanate (MDI: 0.5 wt% of the total resin) afforded PLA/PBAT-MDI triblock copolymers. The triblock copolymers were melt-blended to improve the interfacial adhesion of PLA/BF and thus obtain excellent performance of the PLA-ternary polymers. This work presents the first investigation on the effects of PLA/PBAT-MDI triblock copolymers as compatibilizers for PLA/BF blends. The resultant mechanics, the morphology, interface, crystallinity, and thermal stability of the PLA-bio polymers were comprehensively examined via standard characterization techniques. The crystallinity of the PLA-ternary polymers was as high as 43.6%, 1.44× that of PLA/BF, and 163.5% higher than that of pure PLA. The stored energy of the PLA-ternary polymers reached 20,306.2 MPa, 5.5× than that of PLA/BF, and 18.6× of pure PLA. Moreover, the fatigue life of the PLA-ternary polymers was substantially improved, 5.85× than that of PLA/PBAT-MDI triblock copolymers. Thus, the PLA/PBAT-MDI triblock copolymers are compatibilizers that improve the mechanical properties of PLA/BF.
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http://dx.doi.org/10.3390/polym14132734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269499PMC
July 2022

STAT3 in tumor fibroblasts promotes an immunosuppressive microenvironment in pancreatic cancer.

Life Sci Alliance 2022 11 8;5(11). Epub 2022 Jul 8.

Hollings Cancer Center and Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA

Pancreatic ductal adenocarcinoma (PDAC) is associated with an incredibly dense stroma, which contributes to its recalcitrance to therapy. Cancer-associated fibroblasts (CAFs) are one of the most abundant cell types within the PDAC stroma and have context-dependent regulation of tumor progression in the tumor microenvironment (TME). Therefore, understanding tumor-promoting pathways in CAFs is essential for developing better stromal targeting therapies. Here, we show that disruption of the STAT3 signaling axis via genetic ablation of in stromal fibroblasts in a PDAC mouse model not only slows tumor progression and increases survival, but re-shapes the characteristic immune-suppressive TME by decreasing M2 macrophages (F480+CD206+) and increasing CD8 T cells. Mechanistically, we show that loss of the tumor suppressor PTEN in pancreatic CAFs leads to an increase in STAT3 phosphorylation. In addition, increased STAT3 phosphorylation in pancreatic CAFs promotes secretion of CXCL1. Inhibition of CXCL1 signaling inhibits M2 polarization in vitro. The results provide a potential mechanism by which CAFs promote an immune-suppressive TME and promote tumor progression in a spontaneous model of PDAC.
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http://dx.doi.org/10.26508/lsa.202201460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270499PMC
November 2022

Electron Transfer under the Floquet Modulation in Donor-Bridge-Acceptor Systems.

J Phys Chem A 2022 Jul 4;126(27):4554-4561. Epub 2022 Jul 4.

Department of Chemical Physics, University of Science and Technology of China, Hefei, Anhui 230026, China.

Electron transfer (ET) processes are of broad interest in modern chemistry. With the advancements of experimental techniques, one may modulate the ET via such events as light-matter interactions. In this work, we study the ET under a Floquet modulation occurring in the donor-bridge-acceptor systems, with the rate kernels projected out from the exact dissipaton equation of motion formalism. This together with the Floquet theorem enables us to investigate the interplay between the intrinsic non-Markovianity and the driving periodicity. The observed rate kernel exhibits a Herzberg-Teller-like mechanism induced by the bridge fluctuation subject to effective modulation.
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http://dx.doi.org/10.1021/acs.jpca.2c03308DOI Listing
July 2022

Psychometric evaluation of the pregnancy-related anxiety questionnaire-revised 2 for Chinese pregnant women.

Midwifery 2022 Sep 25;112:103411. Epub 2022 Jun 25.

Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, GA 30322, United States.

Introduction: Pregnancy related anxiety (PrA) is a unique type of anxiety during pregnancy. PrA can affect both maternal and infant health, as well as pregnancy outcomes. The purpose of this study was to culturally translate the Pregnancy-Related Anxiety Questionnaire-Revised 2 (PRAQ-R2) into Chinese and evaluate its psychometric properties in Chinese women during pregnancy irrespective of parity.

Methods: There were 411 Chinese pregnant women recruited from September 2020 to June 2021. The Cronbach's alpha, split-half reliability, and test-retest reliability were used to evaluate the reliability of PRAQ-R2 Chinese version. Validity was analyzed through content validity, convergent validity, discriminant validity and construct validity.

Results: The Chinese version of PRAQ-R2 showed good reliability and validity. The Cronbach's alpha coefficients of the total scale and subscales were all greater than 0.7. The median score (interquartile range, IQR) of PRAQ-R2 was 27 (32, 22). The correlation coefficient between item and hypothesis subscale were all greater than 0.40, indicating good convergence validity. Our findings revealed three dimensions of this scale by exploratory factor analysis and confirmed the original version of PRAQ-R2: worries about bearing a handicapped child, concern about own appearance, and fear of giving birth, with a total variance of 72.84% explained. The confirmatory factor analysis showed accepted model fit indexes.

Limitations: All pregnant women were recruited from one region in China, and the study lacked the longitudinal design. Future longitudinal multi-central studies are needed.

Conclusion: The Chinese version of PRAQ-R2 can be used as a promising tool to measure PrA in Chinese pregnant women without comorbidities and complications.
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http://dx.doi.org/10.1016/j.midw.2022.103411DOI Listing
September 2022

CLEC1B is a Promising Prognostic Biomarker and Correlated with Immune Infiltration in Hepatocellular Carcinoma.

Int J Gen Med 2022 16;15:5661-5672. Epub 2022 Jun 16.

Department of Hepatobiliary Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, People's Republic of China.

Purpose: C-type lectin domain family 1 member B (CLEC1B) is a protein-coding gene involved in various processes, such as platelet activation, tumor cell metastasis and separation of blood/lymphatic vessels. However, how CLEC1B plays its role in hepatocellular carcinoma (HCC) has not been well studied. The purpose of this study was to investigate the clinical significance and biological function of CLEC1B in HCC.

Patients And Methods: Based on (The Cancer Genome Atlas) TCGA database, CLEC1B expression matrix and corresponding clinical information were extracted. ROC curves and Kaplan-Meier method were generated to evaluate the value of CLEC1B as a diagnostic and prognostic biomarker. Moreover, single-gene difference analysis constructed by DESeq2 method and then the related genes were used to predict CLEC1B-related signaling pathways. The ssGSEA algorithm was conducted for studies related to immune infiltration. CLEC1B protein expression was evaluated and immunohistochemistry in HCC tissues through tissue microarray. Finally, the relationship between CLEC1B expression and T cell infiltration was assessed according to tissue microarray.

Results: The mRNA and protein levels of CLEC1B were significantly down-regulated in HCC compared to paired normal tissues, which were further verified in clinical tissue samples. ROC curves and Kaplan-Meier survival analysis suggested the significant diagnostic and clinical prognostic value of CLEC1B. Meanwhile, downregulation of CLEC1B was significantly associated with clinical parameters such as clinical tumor vascular invasion and distant metastasis. Moreover, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene set enrichment (GSEA) analysis indicated that CLEC1B has significant association with immune function. Finally, immune infiltration analysis indicated that CLEC1B was significantly associated with immune cell subsets and affected the efficacy of immunotherapy in cancer patient.

Conclusion: Collectively, our findings suggested that CLEC1B could be a promising prognostic biomarker in HCC and its expression was related to immune cell infiltration.
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http://dx.doi.org/10.2147/IJGM.S363050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208739PMC
June 2022

Impact of centralization of prostate cancer services on the choice of radical treatment.

BJU Int 2022 Jun 20. Epub 2022 Jun 20.

Department of Health Services Research and Policy, London School of Hygiene and Tropical Medicine, London, UK.

Objective: To assess the impact of centralization of prostate cancer surgery and radiotherapy services on the choice of prostate cancer treatment.

Patients And Methods: This national population-based study used linked cancer registry data and administrative hospital-level data for all 16 621 patients who were diagnosed between 1 January 2017 and 31 December 2018 with intermediate-risk prostate cancer and who underwent radical prostatectomy (RP) or radical radiation therapy (RT) in the English National Health Service (NHS). Travel times by car to treating centres were estimated using a geographic information system. We used logistic regression to assess the impact of the relative proximity of alternative treatment options on the type of treatment received, with adjustment for patient characteristics.

Results: Of the 78 NHS hospitals that provide RT or RP for prostate cancer, 41% provide both, 36% provide RT and 23% provide RP. Compared to patients who had both treatment options available at their nearest centre where overall 57% of patients received RT and 43% RP, patients were less likely to receive RT if their nearest centre offered RP only and the extra travel time to a hospital providing RT was >15 min (52% of patients received RT and 48% RP%, odds ratio [OR] 0.70 (0.58-0.85); P < 0.001). Conversely, patients were more likely to receive RT if their nearest centre offered RT and the extra travel time to a hospital providing RP was >15 min (63% of patients received RT and 37% RP, OR 1.23 (1.08-1.40); P < 0.001). There was a negligible impact on the type of treatment received if centres providing alternative treatment options were ≤15-min travel time from each other.

Conclusion: The relative proximity of prostate cancer treatment options to a patient's residence is an independent predictor for the type of radical treatment received. Centralization policies for prostate cancer should not focus on one treatment modality but should consider all treatments to avoid a negative impact on treatment choice.
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http://dx.doi.org/10.1111/bju.15830DOI Listing
June 2022

Comprehensive Analysis of circRNAs, miRNAs, and mRNAs Expression Profiles and ceRNA Networks in Decidua of Unexplained Recurrent Spontaneous Abortion.

Front Genet 2022 31;13:858641. Epub 2022 May 31.

NHC Key Laboratory of Male Reproduction and Genetics, Guangzhou, China.

The diagnosis and treatment of unexplained recurrent spontaneous abortion (URSA) are subject to debate, because the exact underlying mechanisms remain unclear. To address this issue, we elucidated the expression profiles of dysregulated circRNAs, miRNAs, and mRNAs and constructed circRNA-associated competitive endogenous RNA (ceRNA) networks by comparing the decidua of URSA with that of normal early pregnancy (NEP) using RNA-sequencing. In total, 550 mRNAs, 88 miRNAs, and 139 circRNAs were differentially expressed (DE) in decidua of URSA. Functional annotation revealed that DE mRNAs as well as potential target genes of DE miRNAs and DE circRNAs are mainly involved in immunologic function, such as antigen processing and presentation, allograft rejection, and T cell receptor signaling pathway. In addition, the top hub genes, including and , were identified. The mRNAs involved in ceRNA network were enriched in complement and coagulation cascades and protein processing in the endoplasmic reticulum. We found that circRNAs in the ceRNA network, which acted as decoys for hsa-miR-204-5p, were positively correlated with expression. Collectively, the results demonstrated that circRNAs, miRNAs, and mRNAs were aberrantly expressed in the decidua of patients with URSA and played a potential role in the development of URSA. Thus, the establishment of the ceRNA network may profoundly affect the diagnosis and therapy of URSA in the future.
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http://dx.doi.org/10.3389/fgene.2022.858641DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194479PMC
May 2022

Noncritical phase-matching fourth- and fifth-harmonic generation of 1077 nm laser using KDP-family crystals.

Opt Lett 2022 Jun;47(12):2947-2950

We systematically demonstrated the angular and temperature acceptances of noncritical phase-matching (NCPM) fourth- and fifth-harmonic generation (FHG and FiHG) of a 1077 nm laser in NHHPO (ADP), KHPO (KDP), and KDPO (DKDP) crystals. In this work, a new, to the best of our knowledge, laser frequency with a wavelength of 1077 nm was generated by optical parametric amplification, in which the pump light (526.3 nm) was generated by the frequency doubling of a Nd:YLF laser (1052.7 nm), and the signal light was a Yb:YAG laser (1029.5 nm). Subsequently, the 1077 nm laser was used as the fundamental wave for FHG and FiHG to obtain a deep-ultraviolet laser source. For ADP and DKDP crystals, NCPM FHG of a 1077 nm laser was realized at 74.0C and 132.5C, respectively, and large angular acceptances of 59.8 and 61.6 mrad were measured. For the FiHG, NCPM was realized in a KDP crystal at 48.5C with an angular acceptance of 56.4 mrad. The results pave the way for high-energy and high-power deep-ultraviolet laser generation using KDP-family crystals under noncryogenic conditions.
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http://dx.doi.org/10.1364/OL.458952DOI Listing
June 2022

Long non-coding RNA brain cytoplasmic RNA 1 induces cisplatin-resistance of cervical cancer cells by sponging microRNA-330-5p and upregulating high-mobility group box 3.

Gynecol Obstet Invest 2022 Jun 15. Epub 2022 Jun 15.

Objectives: To find out the function of long non-coding RNA (lncRNA) brain cytoplasmic RNA 1 (BCYRN1) in cisplatin (DDP)-resistance of cervical cancer (CC) cells. Design and Materials, Setting, Methods: BCYRN1 expression in CC and DDP-resistant cells was evaluated, with the association of BCYRN1 and prognosis analyzed. Then, DDP-resistant cells with BCYRN1 knockdown were established and the DDP-resistance of these cells was assessed. BCYRN1 subcellular localization was detected and confirmed. Besides, binding relation of BCYRN1 and microRNA (miR)- 330-5p and between miR-330-5p and high-mobility group box 3 (HMGB3) were examined and verified. Moreover, role of miR-330-5p and HMGB3 in the mechanism of BCYRN1 modulating DDP-resistance of CC cells was detected. In addition, xenograft transplantation was conducted to confirm the effect of BCYRN1 in CC cell DDP-resistance.

Results: BCYRN1 was overexpressed in CC, which resulted in poor prognosis and DDP-resistance. BCYRN1 knockdown in DDP-resistant cells downregulated DDP-resistance. Mechanically, BCYRN1 sponged miR-330-5p to strengthen HMGB3 mRNA level. Besides, miR-330-5p underexpression or HMGB3 overexpression reversed the function of BCYRN1 knockdown in inhibiting DDP-resistance of CC cells. Eventually, BCYRN1 knockdown reduced DDP-resistance of CC cells in vivo.

Limitations: There are still some deficiencies in the research; for example, whether there are other miRs working as the downstream genes of BCYRN1 in the ceRNA interaction is not fully clarified; nor the other downstream mechanism of miR-330-5p. Besides, the experimental findings and application into practice need extensive validation.

Conclusions: BCYRN1 knockdown could disrupt DDP-resistance of CC cells through upregulating miR-330-5p to suppress HMGB3 mRNA level.
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http://dx.doi.org/10.1159/000525437DOI Listing
June 2022

Expression and biological activity of lytic proteins HolST-3 and LysST-3 of Salmonella phage ST-3.

Microb Pathog 2022 Aug 10;169:105624. Epub 2022 Jun 10.

College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China. Electronic address:

Salmonella infection is a major public health concern. Several strategies for Salmonella infection prevention and control are currently available including vaccines and antibiotics. However, vaccines are expensive and inefficient, and the use of antibiotics can lead to antibiotic resistance. Thus, alternative strategies for the treatment of Salmonella remain warrant. In this study, recombinant holin HolST-3 and lysin LysST-3 from Salmonella phage ST-3 were expressed and purified, and their bactericidal properties were analyzed. HolST-3 and LysST-3 possessed a wider lysis spectrum and more efficient bactericidal effect than phage ST-3, and a synergistic bactericidal effect was observed when combined in vitro. In addition, we explored the bactericidal properties of HolST-3 and LysST-3 in vivo using zebrafish as a model organism, and found that the bactericidal effects of both HolST-3 and LysST-3 in vivo were comparable to those of cefotaxime, an antibiotic. This study provides a basis for the development of HolST-3 and LysST-3 as novel bactericidal agents for the prevention and treatment of infectious diseases caused by Salmonella spp.
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http://dx.doi.org/10.1016/j.micpath.2022.105624DOI Listing
August 2022

Local Administration of Ginkgolide B Using a Hyaluronan-Based Hydrogel Improves Wound Healing in Diabetic Mice.

Front Bioeng Biotechnol 2022 25;10:898231. Epub 2022 May 25.

School of Pharmaceutical Sciences, Jilin University, Changchun, China.

The delayed and incomplete healing of diabetic wounds remains a major concern of global healthcare. The complex biological processes within the diabetic wound, such as chronic inflammation, impaired blood vessel growth and immature collagen remodeling, dramatically cause the failure of current treatments. Thus, emerging therapeutic strategies are highly desirable. Ginkgolide B (GB, a natural product extracted from the leaves of Ginkgo biloba L.) has been applied in the treatment of cerebrovascular and cardiovascular disorders, which is mainly due to the anti-oxidative, anti-inflammatory and proliferative effects. In this study, the role of GB in facilitating the anti-inflammatory and pro-healing effects on diabetic wounds was for the first time confirmed using , and experimental methods. As a consequence, GB was able to significantly achieve the activities of anti-inflammation, re-epithelialization, and pro-angiogenesis. Previously, a hydrogel has been developed using the high molecular weight hyaluronan (hyaluronic acid, HA) in our laboratory. In this study, this hydrogel was utilized for local administration of GB to the full-thickness wounds of diabetic mice. The resultant hydrogel formulation (HA-GB) resulted in the reduction of inflammation, the enhancement of re-epithelialization and angiogenesis, and the modulation of collagens from type III to type I, significantly promoting the healing outcome as compared with a commercially available wound dressing product (INTRASITE Gel). This study confirms a great therapeutic promise of HA-GB for the chronic wounds of diabetic patients.
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http://dx.doi.org/10.3389/fbioe.2022.898231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174682PMC
May 2022

Psychometric evaluation of the body understanding measure pregnancy scale Chinese version for pregnant Chinese women.

Midwifery 2022 Sep 2;112:103394. Epub 2022 Jun 2.

Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, GA, 30322, USA.

Objectives: At present, there is limited research on pregnancy body image and no valid tool to measure body image in pregnant Chinese women. The purpose of this study was to translate the Body Understanding Measure Pregnancy Scale into Chinese to determine its reliability and validity in measuring body image in pregnant women.

Methods: The translation of the Body Understanding Measure Pregnancy Scale (BUMPs) was carried out with a standardized procedure. This study was conducted from July to December 2020 in the outpatient department of three tertiary hospitals in China. There were 1069 pregnant women completed the questionnaire and 1057 of those women were included in the analysis. Internal consistency, test-retest reliability, content validity, and construct validity of the translation version were examined.

Results: The final Chinese version of the BUMPs (BUMPs-C) had four dimensions with 16 items. Exploratory factor analysis obtained a three-factor solution, which explained 50.26% of the total variances. Confirmatory factor analysis showed that the model fit of the four-factor model was better than the three-factor model, and the four-factor model also reached a satisfactory model fit after modifying: Minimum Discrepancy was 2.82; Comparative Fit Index was 0.939, and Tucker-Lewis Index was 0.92; Root Mean Square Error of Approximation was 0.059. The content validity index of the scale was 1.0; the Cronbach's alpha (α) coefficient was 0.82 for the total scale, the McDonald's omega (ω) coefficient was 0.829; the test-retest reliability was 0.796.

Conclusions: The BUMPs-C showed good reliability and validity among pregnant Chinese women, which can be used as a simple and valid measurement tool to assess the feelings of pregnant Chinese women on body changes during pregnancy.
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http://dx.doi.org/10.1016/j.midw.2022.103394DOI Listing
September 2022

Application of Sensor Path Weighting RAPID Algorithm on Pitting Corrosion Monitoring of Aluminum Plate.

Materials (Basel) 2022 May 30;15(11). Epub 2022 May 30.

School of Reliability and Systems Engineering, Beihang University, 37 Xueyuan Rd., Haidian District, Beijing 100191, China.

Aluminum alloy is widely used in aerospace structures. However, it often suffers from a harsh corrosion environment, resulting in different damage such as pitting corrosion, which leads to a reduction in the service life of aerospace structures. In the present study, the pitting corrosion with a radius of 1 mm and a depth of 0.6 mm was manufactured using hydrofluoric (HF) acid on a 2024-T3 aluminum alloy plate (400 mm × 400 mm × 2 mm) to simulate the corrosion state of equipment. A signal acquisition system with a square sensor network of 12 piezoelectric transducers (PZTs) was established. The sensor path weighting reconstruction algorithm for the probabilistic inspection of defects (SPW-RAPID) is proposed based on corrosion damage characteristic parameters including signal correlation coefficient (SDC), root mean squared error (RMSE), and signal energy damage index (1) to explore the monitoring efficacy of pitting corrosion. The sensor path weight , which is the product of value coefficient and impact factor , is established to modify the corrosion damage characteristic parameters. The results indicate that the SPW-RAPID algorithm can improve the accuracy and clarity of image reconstruction results based on SDC, RMSE and 1, which can locate the pitting corrosion with a radius of 1 mm and a depth of 0.6 mm, and the positioning error is controlled within 0.1 mm. The research work may provide an available way to monitor tiny corrosion damage on an aluminum alloy structure.
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http://dx.doi.org/10.3390/ma15113887DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181756PMC
May 2022

An accurate prognostic prediction for aneurysmal subarachnoid hemorrhage dedicated to patients after endovascular treatment.

Ther Adv Neurol Disord 2022 1;15:17562864221099473. Epub 2022 Jun 1.

Neurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China.

Background: Endovascular treatment for aneurysmal subarachnoid hemorrhage (aSAH) has high fatality and permanent disability rates. It remains unclear how the prognosis is determined by the complex interaction between clinical severity and aneurysm characteristics.

Objective: This study aimed to design an accurate prognostic prediction model for aSAH patients after endovascular treatment and elucidate the interaction between clinical severity and aneurysm characteristics.

Methods: We used a clinically homogeneous data set with 1029 aSAH patients who received endovascular treatment to develop prognostic models. Aneurysm characteristics were measured by variables, such as aneurysm size, neck size, and dome-to-neck ratio, while clinical severity on admission was measured by both comorbidities and neurological condition. In total, 18 clinical variables were used for prognostic prediction. Considering the imbalance between the favorable and the poor outcomes in this clinical population, both ensemble learning and deep reinforcement learning approaches were used for prediction.

Results: The random forest (RF) model was selected as the best approach for the prognostic prediction for all patients and also for patients with good-grade aSAH. Using an independent test data set, the model made accurate predictions (AUC = 0.869 ± 0.036, sensitivity = 0.709 ± 0.087, specificity = 0.805 ± 0.034) with the clinical severity on admission as a leading contributor to the prediction. For patients with good-grade aSAH, the RF model performed the best (AUC = 0.805 ± 0.034, sensitivity = 0.620 ± 0.172, specificity = 0.696 ± 0.043) with aneurysm characteristics as leading contributors. The classic scoring systems failed in this patient group (AUC < 0.600; sensitivity = 0.000, specificity = 1.000).

Conclusion: The proposed prognostic prediction model outperformed the classic scoring systems for patients with aSAH after endovascular treatment, especially when the classic scoring systems failed to make any informative prediction for patients with good-grade aSAH, who constitute the majority group (79%) of this clinical population.
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http://dx.doi.org/10.1177/17562864221099473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168851PMC
June 2022
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