Publications by authors named "Lu Fan"

591 Publications

COVID-19 Quarantine Reveals Behavioral Changes Effect on Myopia Progression.

Ophthalmology 2021 Apr 12. Epub 2021 Apr 12.

School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China; Institute of Biomedical Big Data, Wenzhou Medical University, Wenzhou, 325027, China.

COVID-19 quarantine provides the largest intervention data of myopia progression in schoolchildren. We found grade is an important risk factor, and COVID-19-induced modifications of student's online time and outdoor activity time sufficiently change myopia progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ophtha.2021.04.001DOI Listing
April 2021

Chinese herb microneedle patch for wound healing.

Bioact Mater 2021 Oct 21;6(10):3507-3514. Epub 2021 Mar 21.

Translational Medicine Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325035, China.

Traditional Chinese medicine and Chinese herbs have a demonstrated value for disease therapy and sub-health improvement. Attempts in this area tend to develop new forms to make their applications more convenient and wider. Here, we propose a novel Chinese herb microneedle (CHMN) patch by integrating the herbal extracts, and , with microstructure of microneedle for wound healing. Such path is composed of sap extracted from the herbal leaves traditional kneading method and solidified by plant ash derived from the brine induced process of tofu in a well-designed mold. Because the leaves of the are rich in pectin and various amino acids, the CHMN could be imparted with medicinal efficacy of heat clearing, detoxicating, detumescence and hemostatic. Besides, with the excellent pharmaceutical activity of Asiatic acid extracted from , the CHMN is potential in promoting relevant growth factor genes expression in fibroblasts and showing excellent performance in anti-oxidant, anti-inflammatory and anti-bacterial activity. Taking advantages of these pure herbal compositions, we have demonstrated that the derived CHMN was with dramatical achievement in anti-bacteria, inhibiting inflammatory, collagen deposition, angiogenesis and tissue reconstruction during the wound closure. These results indicate that the integration of traditional Chinese herbs with progressive technologies will facilitate the development and promotion of traditional Chinese medicine in modern society.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bioactmat.2021.03.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988348PMC
October 2021

One-year myopia control efficacy of spectacle lenses with aspherical lenslets.

Br J Ophthalmol 2021 Apr 2. Epub 2021 Apr 2.

Eye Hospital and School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, Zhejiang, China

Aims: To evaluate the 1-year efficacy of two new myopia control spectacle lenses with lenslets of different asphericity.

Methods: One hundred seventy schoolchildren aged 8-13 years with myopia of -0.75 D to -4.75 D were randomised to receive spectacle lenses with highly aspherical lenslets (HAL), spectacle lenses with slightly aspherical lenslets (SAL), or single-vision spectacle lenses (SVL). Cycloplegic autorefraction (spherical equivalent refraction (SER)), axial length (AL) and best-corrected visual acuity (BCVA) were measured at baseline and 6-month intervals. Adaptation and compliance questionnaires were administered during all visits.

Results: After 1 year, the mean changes in the SER (±SE) and AL (±SE) in the SVL group were -0.81±0.06 D and 0.36±0.02 mm. Compared with SVL, the myopia control efficacy measured using SER was 67% (difference of 0.53 D) for HAL and 41% (difference of 0.33 D) for SAL, and the efficacy measured using AL was 64% (difference of 0.23 mm) for HAL and 31% (difference of 0.11 mm) for SAL (all p<0.01). HAL resulted in significantly greater myopia control than SAL for SER (difference of 0.21 D, p<0.001) and AL (difference of 0.12 mm, p<0.001). The mean BCVA (-0.01±0.1 logMAR, p=0.22) and mean daily wearing time (13.2±2.6 hours, p=0.26) were similar among the three groups. All groups adapted to their lenses with no reported adverse events, complaints or discomfort.

Conclusions: Spectacle lenses with aspherical lenslets effectively slow myopia progression and axial elongation compared with SVL. Myopia control efficacy increased with lenslet asphericity.

Trial Registration Number: ChiCTR1800017683.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1136/bjophthalmol-2020-318367DOI Listing
April 2021

From Establishing a World-Renowned Eye Institute to Integrating Ophthalmology and Optometry in China: The Story of The Eye Hospital of Wenzhou Medical University.

Asia Pac J Ophthalmol (Phila) 2021 Mar-Apr 01;10(2):135-141

School of Ophthalmology and Optometry, Eye Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Abstract: The Eye Hospital of Wenzhou Medical University (the Eye Hospital) has found its ways to thrive within the contexts of China to become one of the world-leading institutes in providing ophthalmology and optometry training, education, research, and clinical care at the international standards within 2 decades. It is also the only eye institute in China that possesses 3 national-level research centers (National Clinical Research Center for Ocular Disease, State Key Laboratory of Ophthalmology, Optometry and Visual Science, and National Engineering Technology Research Center for Ophthalmology and Optometry). The hospital is instrumental to the unique medical program in which medical graduates could be both medical doctors and optometrists. Over 10% of the eye doctors in China were trained by the hospital. The SCI-indexed journal Eye and Vision, which is the top eye journal from China, was established by the Eye Hospital in 2015. Its recent endeavor entitled "Eye Valley" provides a one-stop platform for innovation and technology incubation, basic research to clinical trial, from US Food & Drug Administration (FDA) registration to drug production, etc. This article reviews the history of development of the Eye Hospital and elaborates its spirit of "making the impossible possible", which has guided it all the way through from an unknown regional to a renowned top-tier hospital with national and global recognition as a first-class institution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/APO.0000000000000389DOI Listing
April 2021

Development of an X-Band Reflectarray Antenna for Satellite Communications.

Sci Rep 2021 Mar 22;11(1):6530. Epub 2021 Mar 22.

Beijing Institute of Spacecraft System Engineering, Beijing, 100094, China.

An X-band reflectarray antenna using 16 × 12 double square ring elements for satellite communications is proposed in this paper. The feed is a 4 × 3 elements microstrip patch array designed to create edge taper of approximately - 10 dB. A prototype with right-hand circular polarization (RHCP) is manufactured and tested, and the good agreements between simulations and measurements are demonstrated. The good performance is obtained with the aperture efficiency of 40.7% and the 3-dB gain bandwidth of about 10% which is beneficial to nanosatellites.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-85132-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985410PMC
March 2021

[Pollutant Removal Efficiency of Different Units Along a Mature Landfill Leachate Treatment Process in a Membrane Biological Reactor-Nanofiltration Combined Facility].

Huan Jing Ke Xue 2021 Mar;42(3):1469-1476

Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.

Properties of landfill leachate are complex. Therefore, leachate should be treated by combined processes with both biological and advanced methods. Due to the shortage of engineering-scale assessment data about the pollutant treatment contribution of individual process units, existing optimization methods still lack theoretical support. Here, a membrane biological reactor (MBR)+nanofiltration (NF) system with a capacity of 800 m·d was examined. Conventional physiochemical parameters and fluorescent parameters were examined to analyze the contribution of each process unit to treating mature landfill leachate. Furthermore, the transformation of dissolved organic matter (DOM) was evaluated using excitation emission matrix fluorescence spectroscopy-parallel factor (EEMs-PARAFAC). Results showed that the biological treatment removed soluble nitrogen (dissolved nitrogen, DN) by 74.7%, 54.6% occurred in the first-stage denitrification unit. The external ultrafiltration unit reduced dissolved chemical oxygen demand (COD) and dissolved organic carbon (DOC) by 92.2% and 93.3%, respectively. The nanofiltration unit effectively removed heavy metals and salts. Based on the tracking of DOM using fluorescent parameters, the first-stage denitrification unit was found to remove 75.4% of protein-like substances. The ultrafiltration unit mainly retained DOM with high hydrophilicity, while humus with high aromaticity was mainly retained by nanofiltration. The higher the degree of humification, the better the interception effect that was obtained. This indicates that biological treatment using the MBR process can be simplified, and ultrafiltration should prove reliable at preventing clogging during the treatment of mature landfill leachate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13227/j.hjkx.202007263DOI Listing
March 2021

Low-molecular-weight heparin calcium attenuates the tourniquet-induced ischemia-reperfusion injury in rats.

Injury 2021 Mar 5. Epub 2021 Mar 5.

Department of Orthopaedics, Orthopaedics Key Laboratory of Gansu Province, Orthopaedics Clinical Research Center of Gansu Province, Lanzhou University Second Hospital, Lanzhou, Gansu 730030, China. Electronic address:

Ischemia-reperfusion injury (IRI) is a common postoperative complication of the tourniquet used surgery; low-molecular-weight heparin calcium (LMWH) is frequently used postoperatively to prevent the formation of deep venous thrombosis. However, subcutaneous hemorrhage can usually be seen in patients who underwent lower limb surgery, especially in total knee arthroplasty, the influence of LMWH on IRI remains controversial. In this experiment, we designed an animal model to observe the influence of LMWH on the skeletal muscle injury induced by tourniquets. Sprague-Dawley (SD) rats underwent either 2 h of unilateral hindlimb ischemia or anesthesia alone, at different time points of reperfusion interval, animals received either 4mg/kg LMWH or normal saline subcutaneously twice a day. The levels of inflammatory markers in serum, the expression of apoptosis proteins, as well as histological examination of skeletal muscles, were detected at 48-h reperfusion. We found that the injury of skeletal muscle and the systemic inflammatory response was less severe in LMWH-treated animals, indicating that LMWH could attenuate the tourniquet-induced IRI. In conclusion, LMWH given postoperatively after limb surgery may be clinically beneficial.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.injury.2021.03.006DOI Listing
March 2021

DOM chemodiversity pierced performance of each tandem unit along a full-scale "MBR+NF" process for mature landfill leachate treatment.

Water Res 2021 May 3;195:117000. Epub 2021 Mar 3.

Institute of Waste Treatment and Reclamation, Tongji University, Shanghai, 200092, PR China. Electronic address:

Mature landfill leachate contains a substantial fraction of recalcitrant dissolved organic matters (DOM) that is a challenging for conventional wastewater treatment that is typically focused on the removal of biodegradable organic matter. "Biological treatment + membrane treatment" has been widely employed to treat complex leachate. However, the performance of each unit based on both conventional bulk indicators and molecular information has not been well understood. Therefore, the fate of DOM chemodiversity along the full-scale treatment process across ten sampling points over three different seasons were analyzed to determine the efficiency of every unit process with the assistance of ultra-performance liquid chromatography coupled with hybrid quadrupole Orbitrap mass spectrometry. Results showed that the process performance, visualized through the molecular signals, were relatively stable in the temporal dimension. The process removed 83.2%-92.2% of DOM molecules in terms of richness, where lignin/carboxyl-rich alicyclic compounds (CRAM)-likes with relatively high saturation was preferentially removed, while newly generated bio-derived N-containing compounds (N/C 0.15-0.17) became resistant. The relationship between conventional bulk physicochemical indicators and molecular indexes suggested that soluble chemical oxygen demand (sCOD) and dissolved organic carbon (DOC) were contributed by the refractory DOM with high weighted average double bond equivalents (DBE), which was distributed in the region of O/C 0.2-0.5 and H/C 1.2-1.8. This refractory DOM required ultrafiltration and nanofiltration for removal. DOM molecules were positively correlated with five-day biochemical oxygen demand (BOD) and revealed that approximately 96.9%-98.4% of the DOM could be removed or transformed in the primary anoxic zone. In addition, the bio-derived aliphatic/proteins, lipids and lignin/CRAM-likes (O/C > 0.2) with condensed aromatization were the sources of dissolved organic nitrogen (DON) and still remained in the final effluent. The present study suggests that the design and operation of the combination process with biological and membrane treatment could be specifically optimized based on the DOM molecular characteristics of the wastewater.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.watres.2021.117000DOI Listing
May 2021

Responsive Hydrogel Microcarrier-Integrated Microneedles for Versatile and Controllable Drug Delivery.

Adv Healthc Mater 2021 Mar 9:e2002249. Epub 2021 Mar 9.

Department of Rheumatology and Immunology, Institute of Translational Medicine, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008, China.

Stratagems of drug delivery are important for disease treatment and other biomedical areas. In this study, a novel stratagem is presented for versatile and controllable drug delivery by integrating photoresponsive drug delivery microspheres (PDDMs) into pyramid microneedle (MN) arrays. The PDDMs, containing black-phosphorus (BP) and poly (N-isopropylacrylamide) (pNIPAM), are generated by a flexible capillary microfluidic method. Benefiting from the high water content of the pNIPAM hydrogel, various bioactive substances can be loaded and maintain biological activity. Furthermore, due to the near-infrared (NIR) absorption and conversion capabilities of the contained BP, the PDDMs can increase temperature, shrink volume, and release their encapsulated bioactives under the trigger of biocompatible NIR. In addition, as the PDDMs are stuffed into the solid MN arrays of porous ethoxylated trimethylolpropane triacrylate (ETPTA), the composited PDDMs-MNs system has enough mechanical strength to penetrate into the skin and can deliver drugs underneath the skin uniformly. Based on the resultant PDDMs-MNs, it is demonstrated that insulin can be controllably released to adjust blood glucose levels of streptozotocin (STZ)-induced diabetic mice. Thus, it is believed that the PDDMs-MNs can act as an excellent drugs delivery system and will find many practical values in clinical medicine.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adhm.202002249DOI Listing
March 2021

Improvement criteria for different advanced technologies towards bio-stabilized leachate based on molecular subcategories of DOM.

J Hazard Mater 2021 Feb 20;414:125463. Epub 2021 Feb 20.

Institute of Waste Treatment and Reclamation, Tongji University, Shanghai 200092, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, PR China. Electronic address:

Considering dissolved organic matter (DOM) molecules, the present study is an attempt to unravel the individual removal targets of nine advanced treatment technologies for bio-stabilized landfill leachate. For the eight DOM molecular subcategories, preferable technologies and removal rates were as follows: lipids ‒ powdered activated carbon (PAC) adsorption (97%) and Fenton (97%); proteins ‒ extended electrolysis (92%) and Fenton (92%); and lignins/carboxylic rich alicyclic molecules (CRAM)-like organics ‒ Fenton (90%) and extended electrolysis (75%). As to individual technologies, Fenton, PAC adsorption, extended electrolysis, and reverse osmosis (RO) had the highest removal rates based on the intensity and abundance of DOM. As to the improved technology combinations, "Fenton with PAC adsorption" and "PAC adsorption with reverse osmosis" were then recommended according to the target complementarity in compound intensity and abundance. The study suggested that the treatment strategy of an unknown recalcitrantly biodegraded wastewater could be designed in a tailored way based on the subcategorized DOM characteristics of the refractory wastewater.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.125463DOI Listing
February 2021

Flow analysis of major and trace elements in residues from large-scale sewage sludge incineration.

J Environ Sci (China) 2021 Apr 29;102:99-109. Epub 2020 Sep 29.

Institute of Waste Treatment & Reclamation, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.

Increase of sewage sludge (SS) has led to the construction of more incineration plants, exacerbating to the production of SS incineration residues. However, few studies have considered the mass balance of elements in large-scale SS incineration plants, affecting the residues treatment and utilization. In this study, flow analysis was conducted for major and trace elements in the SS, the fly ash (sewage sludge ash, SSA) and bottom ash from two large-scale SS incineration plants. The elemental characteristics were compared with those of coal fly ash (CFA), and air pollution control residues from municipal solid waste incineration (MSWIA), as well as related criteria. The results showed that the most abundant major element in SSA was Si, ranging from 120 to 240 g/kg, followed by Al (76-348 g/kg), Ca (26-113 g/kg), Fe (35-80 g/kg), and P (26-104 g/kg), and the trace elements were mainly Zn, Ba, Cu, and Mn. Not all the major elements were derived from SS. Most trace elements in the SS incineration residues accounted for 82.4%-127% of those from SS, indicating that SS was the main source of trace elements. The partitioning of heavy metals in the SS incineration residues showed that electrostatic precipitator ash or cyclone ash with high production rates were the major pollutant sinks. The differences in some major and trace elements could be indicators to differentiate SSA from CFA and MSWIA. Compared with related land criteria, the pollutants in SSA should not be ignored during disposal and utilization.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jes.2020.09.023DOI Listing
April 2021

Fluid shear stress-induced down-regulation of microRNA-140-5p promotes osteoblast proliferation by targeting VEGFA via the ERK5 pathway.

Connect Tissue Res 2021 Mar 8:1-13. Epub 2021 Mar 8.

Department of Orthopaedics, Lanzhou University Second Hospital, Lanzhou, Gansu, China.

: Fluid shear stress (FSS) plays a critical role in osteoblast proliferation. However, the role of miRNA in osteoblast proliferation induced by FSS and the possible molecular mechanisms remain to be defined. The aim of the present study was to investigate whether miR-140-5p regulates osteoblast proliferation under FSS and its molecular mechanism.: miR-140-5p expression was measured by qRT-PCR. Western blot was used to measure the expressions of P-ERK1/2, ERK1/2, P-ERK5 and ERK5. The levels of VEGFA, PCNA, CDK4 and Cyclin D1 were identified through qRT-PCR and western blot, respectively. Cell proliferation was detected by CCK-8 assay and EdU labeling assay. Dual-luciferase reporter assay was used to validate the target of miR-140-5p.: miR-140-5p was significantly down-regulated when MC3T3-E1 cells were exposed to FSS. We then confirmed that up-regulation of miR-140-5p inhibited and down-regulation of miR-140-5p promoted osteoblast proliferation. In addition, FSS promotes osteoblast proliferation via down-regulating miR-140-5p. Luciferase reporter assay demonstrated that VEGFA is a direct target of miR-140-5p. Furthermore, transfection of mimic-140-5p inhibited the up-regulation of VEGFA protein level induced by FSS, suggesting that FSS regulates VEGFA protein expression via miR-140-5p. Further investigations demonstrated that VEGFA could promote osteoblast proliferation. Lastly, we demonstrated that miR-140-5p regulates osteoblast proliferation and ERK5 activation through VEGFA.: Our study demonstrates that FSS-induced the down-regulation of miR-140-5p promotes osteoblast proliferation through activing VEGFA/ERK5 signaling pathway. These findings may provide a novel mechanism of FSS-induced osteoblast proliferation and offer a new avenue to further investigate osteogenesis induced by mechanical loading.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/03008207.2021.1891228DOI Listing
March 2021

Upcycling of PET waste into methane-rich gas and hierarchical porous carbon for high-performance supercapacitor by autogenic pressure pyrolysis and activation.

Sci Total Environ 2021 Jun 2;772:145309. Epub 2021 Feb 2.

Institute of Waste Treatment & Reclamation, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, PR China; Shanghai Institute of Pollution Control and Ecological Security, 1239 Siping Road, Shanghai 200092, PR China. Electronic address:

The explosive growth of polyethylene terephthalate (PET) wastes has brought serious pollution to the environment. Here, PET waste was upcycled into methane-rich pyrolysis gas and carbon material for energy storage through autogenic pressure pyrolysis and post-activation. The pyrolysis gas contained 34.58 ± 0.23 vol% CH. After CO removal, the high caloric value of the pyrolysis gas could reach 29.2 MJ m, which could be used as a substitute natural gas. Pyrolytic carbon was further activated by KOH and ZnCl. KOH-activated carbon (AC-K) obtained a hierarchical porous structure, a high specific surface area of 2683 m g and abundant surface functional groups. Working as supercapacitor electrodes, AC-K exhibited an outstanding specific capacitance of 325 F g at a current density of 0.5 A g. After 5000 charge-discharge cycles, AC-K still retained 91.86% of the initial specific capacitance. This study provides a sustainable way to control plastic-derived pollution and alleviate the energy crisis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.145309DOI Listing
June 2021

Advances in retina imaging as potential biomarkers for early diagnosis of Alzheimer's disease.

Transl Neurodegener 2021 Feb 1;10(1). Epub 2021 Feb 1.

School of Ophthalmology and Optometry, Wenzhou Medical College, Wenzhou, 325027, China.

As the most common form of dementia, Alzheimer's disease (AD) is characterized by progressive cognitive impairments and constitutes a major social burden. Currently, the invasiveness and high costs of tests have limited the early detection and intervention of the disease. As a unique window of the brain, retinal changes can reflect the pathology of the brain. In this review, we summarize current understanding of retinal structures in AD, mild cognitive impairment (MCI) and preclinical AD, focusing on neurodegeneration and microvascular changes measured using optical coherence tomography (OCT) and optical coherence tomography angiography (OCTA) technologies. The literature suggests that the impairment of retinal microvascular network and neural microstructure exists in AD, MCI and even preclinical AD. These findings provide valuable insights into a better understanding of disease pathogenesis and demonstrate that retinal changes are potential biomarkers for early diagnosis of AD and monitoring of disease progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40035-021-00230-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849105PMC
February 2021

Evaluation of the efficacy of posterior scleral contraction in the treatment of macular hole with retinal detachment in high myopia Macular surgery on sclera.

Retina 2021 Jan 21. Epub 2021 Jan 21.

School of Ophthalmology and Optometry, Wenzhou Medical University, 270 Xueyuan Road, Wenzhou, Zhejiang, China, 325027.

Purpose: To evaluate efficacy of posterior scleral contraction (PSC) for macular hole with retinal detachment (MHRD) in high myopia.

Methods: Seventy-three MHRD eyes were treated with PSC. A strip was sent across inferior-temporal scleral surface to posterior pole, then two ends were led out from nasal-inferior and temporal-superior areas. It was tightened to contract posterior sclera with designed axial length (AL) shortening ([10% of pre-operative AL-0.5]mm) after aqueous humors drained from anterior chamber. Recovery was classified as type I (retinal reattachment with MH bridged) and II (retinal reattachment without MH bridged). Follow-up duration was 25.3±18.0 months.

Results: AL was 30.01±2.27mm at pre-operation and shortened by 2.57±0.82mm intra-operatively. At final post-operation, maintained AL shortening was 1.87±0.92mm. Forty-six eyes (63.0%) recovered as type I and 26 eyes (35.6%) as type II, 1 eye (1.4%) unrecovered. Post-operative best-corrected visual acuity (BCVA, logarithm of minimal angle of resolution [LogMAR]) was better than pre-operative one (0.85±0.50 [Snellen 20/125] vs. 1.11±0.56 [Snellen 20/250], P<0.001) with correlation (r=0.662, P<0.001) and consistency (Kappa=0.34, P<0.001) between two. BCVA improvement didn't differ between recovery types (P=0.206).

Conclusion: MHRD was successfully recovered by PSC with AL shortening. Visual improvement was achieved, correlated and consistent with pre-operative BCVA while independent of recovery types.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/IAE.0000000000003126DOI Listing
January 2021

Retinal Structural and Microvascular Alterations in Different Acute Ischemic Stroke Subtypes.

J Ophthalmol 2020 9;2020:8850309. Epub 2020 Dec 9.

School of Ophthalmology and Optometry, Wenzhou Medical College, Wenzhou, Zhejiang, China.

Introduction: Retinal structural and microvascular damages reflect damage to cerebral microvasculature and neurons. We aimed to investigate neovascular unit abnormalities among patients with large-artery atherosclerosis (LAA) or small-vessel occlusion (SAA) and control subjects.

Methods: Twenty-eight LAA patients, forty-one SAA patients, and sixty-five age- and gender-matched controls were recruited. Based on optical coherence tomography angiography (OCTA), retinal capillary vessel density was assessed in the general and local sectors, and the thickness of individual retinal layer was extracted from retinal structural images. The differences between structural and microvascular were analyzed.

Results: The superior peripapillary retinal nerve fiber layer (pRNFL) thickness was significantly different among the three groups, and the LAA group had the thinnest thickness. Compared to the control group, the deep retinal capillary vessel density in other two stroke subgroups were significantly reduced in all regions except in the inferior region ( < 0.05), and the fractal dimension in C2 and C4 regions of deep retina was significantly lower in the LAA group ( < 0.05). Compared with superficial microvascular network, deep microvascular network is more sensitive to ischemic stroke. In addition, we have demonstrated quadrant-specific pRNFL abnormalities in LAA and SAA patients. Superior quadrant pRNFL thickness differences between stroke subgroups may suggest that changes in retinal nerve fiber layer are more sensitive to subtype identification than changes in retinal microvascular structure. All in all, the alteration in retinal structural and microvascular may further elucidate the role of the neovascular unit in ischemic stroke, suggesting that the combination of these two indicators could be used for subtype identification to guide prognosis and establish a risk prediction model.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/8850309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803129PMC
December 2020

Degradation of organic dyes by peroxymonosulfate activated with water-stable iron-based metal organic frameworks.

J Colloid Interface Sci 2021 May 5;589:298-307. Epub 2021 Jan 5.

Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Function Molecules, Hubei University 430062, People's Republic of China. Electronic address:

In this study, MIL-101(Fe) is prepared via a facile solvent-thermal method, and then applied as catalyst for activating PMS, thus generating reactive radicals for organic dyes removal in aqueous solution. Rhodamine B (RhB), Malachite Green (MG) and Methylene Blue (MB) were selected as model pollutants. The experiments showed that MIL-101(Fe)/PMS had higher removal efficiency for dyes than MIL-101(Fe), indicating that MIL-101(Fe) can be simultaneously used as adsorbent and PMS-activating catalyst for dyes removal. The effects of initial pH and the coexisted negative ions on MB degradation were further discussed, showing that MIL-101(Fe) can degrade MB with excellent stability. Furthermore, the reactive species in MIL-101(Fe)/PMS system were deduced as sulfate radicals and hydroxyl radicals. Finally, the degradation intermediates and possible degradation pathway were investigated by LC-MS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2020.12.123DOI Listing
May 2021

Anomalous Phylogenetic Behavior of Ribosomal Proteins in Metagenome-Assembled Asgard Archaea.

Genome Biol Evol 2021 Jan;13(1)

Institute for Molecular Evolution, Heinrich Heine University Düsseldorf, Germany.

Metagenomic studies permit the exploration of microbial diversity in a defined habitat, and binning procedures enable phylogenomic analyses, taxon description, and even phenotypic characterizations in the absence of morphological evidence. Such lineages include asgard archaea, which were initially reported to represent archaea with eukaryotic cell complexity, although the first images of such an archaeon show simple cells with prokaryotic characteristics. However, these metagenome-assembled genomes (MAGs) might suffer from data quality problems not encountered in sequences from cultured organisms due to two common analytical procedures of bioinformatics: assembly of metagenomic sequences and binning of assembled sequences on the basis of innate sequence properties and abundance across samples. Consequently, genomic sequences of distantly related taxa, or domains, can in principle be assigned to the same MAG and result in chimeric sequences. The impacts of low-quality or chimeric MAGs on phylogenomic and metabolic prediction remain unknown. Debates that asgard archaeal data are contaminated with eukaryotic sequences are overshadowed by the lack of evidence indicating that individual asgard MAGs stem from the same chromosome. Here, we show that universal proteins including ribosomal proteins of asgard archaeal MAGs fail to meet the basic phylogenetic criterion fulfilled by genome sequences of cultured archaea investigated to date: These proteins do not share common evolutionary histories to the same extent as pure culture genomes do, pointing to a chimeric nature of asgard archaeal MAGs. Our analysis suggests that some asgard archaeal MAGs represent unnatural constructs, genome-like patchworks of genes resulting from assembly and/or the binning process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/gbe/evaa238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813640PMC
January 2021

Emb15 encodes a plastid ribosomal assembly factor essential for embryogenesis in maize.

Plant J 2021 Apr 11;106(1):214-227. Epub 2021 Feb 11.

Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao Campus, Qingdao, 266237, China.

Ribosome assembly factors guide the complex process by which ribosomal proteins and the ribosomal RNAs form a functional ribosome. However, the assembly of plant plastid ribosomes is poorly understood. In the present study, we discovered a maize (Zea mays) plastid ribosome assembly factor based on our characterization of the embryo defective 15 (emb15) mutant. Loss of function of Emb15 retards embryo development at an early stage, but does not substantially affect the endosperm, and causes an albino phenotype in other genetic backgrounds. EMB15 localizes to plastids and possesses a ribosome maturation factor M (RimM) domain in the N-terminus and a predicted UDP-GlcNAc pyrophosphorylase domain in the C-terminus. The EMB15 RimM domain originated in bacteria and the UDP-GlcNAc pyrophosphorylase domain originated in fungi; these two domains came together in the ancestor of land plants during evolution. The N-terminus of EMB15 complemented the growth defect of an Escherichia coli strain with a RimM deletion and rescued the albino phenotype of emb15 homozygous mutants. The RimM domain mediates the interaction between EMB15 and the plastid ribosomal protein PRPS19. Plastid 16S rRNA maturation is also significantly impaired in emb15. These observations suggest that EMB15 functions in maize seed development as a plastid ribosome assembly factor, and the C-terminal domain is not important under normal conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/tpj.15160DOI Listing
April 2021

Knowledge and current practices of ICU nurses regarding aerosol therapy for patients treated with invasive mechanical ventilation: a nationwide cross-sectional study.

J Clin Nurs 2021 Jan 13. Epub 2021 Jan 13.

Department of Medical Records, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China.

Background: Aerosol therapy is a routine operation for intensive care unit (ICU) nurses; however, evidence of the knowledge and current practices of ICU nurses regarding aerosol therapy for patients with invasive mechanical ventilation is insufficient in China.

Objective: This study aimed to determine the knowledge and current practices of ICU nurses regarding aerosol therapy for patients with invasive mechanical ventilation in China.

Setting: A total of 433 hospitals in 92 cities (including 31 capital cities) in 31 provinces in China participated in the study.

Methods: A questionnaire was used to investigate the knowledge and current practices of ICU nurses regarding aerosol therapy for patients treated with invasive mechanical ventilation, including 42 questions covering five aspects: sociodemographic information, aerosolization devices, atomised drugs, atomisation operation, and atomisation-related knowledge. Descriptive analyses of the distribution of the sample are reported as percentages and medians. Univariate and multivariate analysis was used to detect the factors of the interviewee's atomisation knowledge and practices scores. A STROBE checklist was used to guide the reporting of the research.

Results: Of the 1,995 questionnaires that were returned, 1,978 were analysed. Bronchodilators and glucocorticoids were the most frequently administered drugs. Seventy-four percent of the total respondents reported placing a filter on the expiratory limb during aerosol therapy, and 47% of these reported that the filter was changed once a day. Only 13% of the respondents reported always turning the heating humidifier off during aerosol therapy, and 48% never did. Knowledge about the optimal droplet size or atomisation yield was poor. Work experience in the ICU and frequency of atomisation training were the independent influencing factors for atomisation knowledge and practice scores (F=279.653, P<0.001; F=120.556, P<0.001, respectively).

Conclusions: The knowledge of ICU nurses about the optimal implementation of aerosol therapy is poor, and the current scientific knowledge about optimal implementation seemed to be applied infrequently. Atomisation-related training should be strengthened, especially for nurses with junior titles and with less work experience.

Relevance To Clinical Practice: Improving the level of ICU nurses' atomization practice ability is helpful to ensure patient safety. In clinical work, atomization expert consensus can be used to carry out relevant training and standardize atomization operation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jocn.15639DOI Listing
January 2021

The effect of nerve growth factor on corneal nerve regeneration and dry eye after LASIK.

Exp Eye Res 2021 02 2;203:108428. Epub 2021 Jan 2.

Eye Hospital and School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, Zhejiang, China. Electronic address:

Dry eye is the most common complication after refractive surgery, especially after laser in situ keratomileusis (LASIK), in which nerves may be cut when making the corneal flap. Nerve growth factor (NGF) has been demonstrated to stimulate corneal sensitivity and nerve regeneration and NGF has been suggested as a potential treatment for dry eye disease (DED). Hence, this study aimed to investigate the effect of NGF on corneal nerve regeneration, sensitivity and dry eye symptoms after LASIK, compared to hycosan and normal saline (NS) treatments. Thirty-eight New Zealand white rabbits that underwent LASIK procedures were randomly assigned to three groups. Each group underwent NGF, hycosan, and NS treatment. The nerve densities and the number of corneal sub-basal and superficial stromal nerves were measured with confocal microscopy, and the results were compared before surgery and at one month and three months postoperatively. Corneal sensitivity was assessed with an esthesiometer. The tear breakup time (TBUT) was recorded to check for signs of dry eye. The whole corneas of the experimental animals were excised at three months after the surgery for immunohistochemically analysis. After LASIK, treatment with NGF significantly accelerated the recovery of sub-basal and superficial stromal nerve densities and the numbers, compared to hycosan and NS treatments at one month and three months postoperatively (NGF vs. hycosan, P < 0.01 each; NGF vs. NS, P < 0.01 each). The recovery of corneal sensitivity was significantly enhanced in the NGF group compared to the hycosan or NS treatment groups after surgery (P < 0.05). Also, the TBUT data showed a statistically significant longer time in the NGF group at one month, and three months postoperatively (P < 0.05). Immunofluorescence analysis showed the nerve fiber quantity of the NGF group was larger than in the hycosan and NS groups. Taken together, the experimental results suggested that mNGF had an obvious effect on promoting corneal nerve repairing and the potential to improve dry eye in different periods following LASIK.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.exer.2020.108428DOI Listing
February 2021

Technical Report: A New Device Attached to a Smartphone for Objective Vision Screening.

Optom Vis Sci 2021 Jan;98(1):18-23

Wenzhou Medical University, Wenzhou, Zheniang, China.

Significance: A new device attached to a smartphone was created for objective vision screening of young children including infants and newborns. The device is compact, lightweight, portable, cost-effective, and easy to operate. Therefore, it is suitable for screening large numbers of children in clinical settings, schools, and communities.

Purpose: This article introduces a new device attached to a smartphone for objective vision screening. It can detect and categorize significant refractive errors, anisometropia, strabismus, cloudy ocular media, and ptosis that may cause amblyopia.

Methods: The new device applies the same principles as conventional streak retinoscopy but examines both eyes simultaneously and records the results electronically. The device comprises optical elements that produce a precise streak light beam and move it across a child's both eyes. The smartphone's video camera catches and records the motion of retinal reflex inside the child's pupils. By observing the direction of motion of the retinal reflex relative to the light beam motion, as well as its speed, width, and brightness, the examiner is able to assess the individual and comparative refractive status, ocular alignment, and other conditions.

Results: Vision screening with this device does not require any subjective response from children. The examination can be performed and analyzed by nonprofessionals after a short learning period of time. Because the examination results are electronically recorded by the smartphone, they can be stored in the child's files and sent out for professional consultations.

Conclusions: The new device will provide the same functions as conventional streak retinoscopy but examines a child's both eyes simultaneously, so that, in addition to categorizing refractive errors and assessing clarity of refractive media of the eyes, it can also detect anisometropia, strabismus, and anisocoria. In addition to showing the examination results on the smartphone's screen, the device can also store the results electronically.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/OPX.0000000000001621DOI Listing
January 2021

Diurnal variation of corneal elasticity in healthy young human using air-puff optical coherence elastography.

J Biophotonics 2021 Jan 2. Epub 2021 Jan 2.

School of Ophthalmology and Optometry, Wenzhou Medical University, Zhejiang, China.

Due to the disruption of intraocular pressure (IOP) and central corneal thickness (CCT), diurnal variation in normal young human corneal elasticity is not clear. Using the custom-built air-puff optical coherence elastography, one eye of twenty-one normal subjects is enrolled randomly to measure the central corneal elasticity, IOP, and CCT in different time points within a day. Based on the multi-level model, the corneal elastic modulus is found to have a linear positive relation with IOP (P<0.01) but not CCT (P=0.175) and time point (P=0.174-0.686). A new indicator, corneal elasticity change rate, is proposed to present the magnitude of corneal elasticity change caused by 1 mmHg IOP, which can correct the interference effect of IOP. The results show that the corneal elasticity in the normal young human doesn't have the characteristics of diurnal variation under IOP control. Furthermore, IOP plays an important role in the corneal elasticity, and corneal elasticity change rate can increase the comparability of results between individuals. This article is protected by copyright. All rights reserved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jbio.202000440DOI Listing
January 2021

The Jidong Eye Cohort Study: objectives, design, and baseline characteristics.

Eye Vis (Lond) 2020 Dec 29;7(1):58. Epub 2020 Dec 29.

Eye Hospital and School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, Zhejiang, China.

Background: To describe the objective, design and baseline characteristics of the Jidong Eye Cohort Study (JECS), a community-based cohort in China based on etiology, imaging and biomarkers. The JECS will clarify the pathogenesis of visual impairment and status of ocular indicators in the occurrence and progression of cardio-cerebrovascular and neurological diseases.

Methods: Between August 2019 and January 2020, the JECS recruited consecutive participants aged 18 years and older from the Jidong communities in China. The demographic and clinical characteristics were collected by trained site personnel via face-to-face interviews. The relevant biological samples were also collected. The participants underwent comprehensive ophthalmic examination, such as retinal photography and optical coherence tomography (OCT) angiography. The following outcomes were measured annually: ocular vascular abnormality, optic nerve degeneration, cardiovascular diseases (CVD) and neurological diseases. The study will be performed until 2024.

Results: Among 3377 participants, the average age was 45.0 ± 12.5 years and 1809 (53.6%) were women. Hypertension occurred in 825 individuals (25.0%), diabetes in 258 (7.7%), hyperglycemia in 474 (14.2%), and a CVD history in 100 (3.0%). The mean best-corrected visual acuity was 0.1 logMAR in the recruited subjects. The average OCT signal index was 8.2 ± 1.2. Additionally, the mean vessel densities for the entire measured area were 46.4% and 50.8% for the superficial and deep vascular complex, respectively. Mean area and perimeter of foveal avascular zone was 0.3 mm and 2.3 mm.

Conclusions: The JECS is a large community-based prospective cohort in North China. Rich data collected from this study will provide the opportunity to identify risk factors, imaging, and biomarkers of visual impairment (either ocular vascular anomalies or optic nerve degeneration) and to evaluate their associations with CVD and neurological diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40662-020-00223-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7771074PMC
December 2020

Interocular Suppression as Revealed by Dichoptic Masking Is Orientation-Dependent and Imbalanced in Amblyopia.

Invest Ophthalmol Vis Sci 2020 Dec;61(14):28

School of Ophthalmology and Optometry, Affiliated Eye Hospital, State Key Laboratory of Ophthalmology, Optometry and Vision Science, Wenzhou Medical University, Wenzhou, Zhejiang, China.

Purpose: We investigate the orientation tuning of interocular suppression using a dichoptic masking paradigm in adult controls and amblyopes.

Methods: Fourteen adults with anisometropic or mixed amblyopia and 10 control adults participated in our study. Contrast sensitivity was measured by presenting a target Gabor in the tested eye and mean luminance in the untested eye (monocular) and by presenting a target in the tested eye and a bandpass oriented filtered noise in the other eye (masked). Interocular suppression was defined as the thresholds difference between the monocular and masked conditions for each eye. Interocular suppression was measured under parallel and orthogonal suppression configurations. The peak spatial frequency of the target and mask was 0.25 c/d in experiment 1 (low), 1.31 c/d in experiment 2 (mid), and 6.87 c/d in experiment 3 (high).

Results: The masking suppression induced by the amblyopic eye was less strong than that induced by the fellow eye. The suppression from the fellow eye was similar to that observed in the controls. Interocular suppression under parallel configuration was less strong than under orthogonal configuration in amblyopes at low and mid spatial frequency, but not at high spatial frequency.

Conclusions: We demonstrate that the abnormal interocular masking in amblyopia displays the expected characteristic of orientation selectivity expected of normal controls at low and mid spatial frequency, but not at high spatial frequency. The dichoptic masking imbalance between the eyes of amblyopes results in a net suppression of the amblyopic eye during binocular viewing, modeling clinical suppression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1167/iovs.61.14.28DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774058PMC
December 2020

Progression of Macular Vessel Density in Primary Open-Angle Glaucoma: A Longitudinal Study.

Am J Ophthalmol 2021 03 24;223:259-266. Epub 2020 Oct 24.

Eye Hospital and School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, Zhejiang, China; National Clinical Research Center for Ocular Diseases, Wenzhou, Zhejiang, China. Electronic address:

Purpose: To evaluate the rate of progression of macular vessel density (mVD) in primary open-angle glaucoma (POAG) and explore the relationship between the progression of mVD and macular ganglion cell-inner plexiform layer (mGCIPL) thickness and parapapillary retinal nerve fiber layer (pRNFL) thickness.

Design: Prospective cohort study.

Methods: In this study, 102 eyes with POAG were followed for 36.6 ± 6.4 months. The rates of progression were estimated by linear models. The agreement of progression detection among the 3 parameters was evaluated with Kappa statistics. The influence of baseline measurements on the rates of progression of mGCIPL thickness, pRNFL thickness, and mVD was investigated by linear mixed modeling. Kaplan-Meier survival analysis was adopted to calculate the survival probabilities.

Results: The respective rate of progression by linear regression was -0.102 ± 0.054 μm/month, -0.160 ± 0.086 μm/month, and -0.199 ± 0.073 %/month for mGCIPL thickness, pRNFL thickness, and mVD. The agreement in detection of progression among them was poor with the Conger's Kappa coefficient of 0.098 (95% confidence interval: -0.025~0.220, P = .116). The significant factors influencing the rate of progression of mVD were baseline mGCIPL thickness, baseline pRNFL thickness, and baseline mVD (P ≤ .001), while baseline mVD was not a significant factor influencing the rates of progression of mGCIPL thickness and pRNFL thickness (P ≥ .659). Also, pRNFL thickness had a better survival probability compared with the other 2 parameters (P = .025).

Conclusions: The mGCIPL thickness, pRNFL thickness, and mVD decreased over time in POAG eyes. The rate of reduction of mVD was significantly influenced by the baseline measurements of mGCIPL thickness, pRNFL thickness, and mVD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ajo.2020.10.008DOI Listing
March 2021

Endophytic fungus Phomopsis liquidambaris B3 induces rice resistance to RSRD caused by Fusarium proliferatum and promotes plant growth.

J Sci Food Agric 2020 Dec 21. Epub 2020 Dec 21.

Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing, China.

Background: Rice spikelet rot disease (RSRD) is an emerging disease that significantly reduces rice yield and quality. In this study, we evaluated the potential use of the broad-spectrum endophytic fungus Phomopsis liquidambaris B3 as a biocontrol agent against RSRD. We also compared the control effects of different treatments, including chemical fungicides and treatment with multiple strains and single strains in combination or individually, against RSRD. The objective of this study was to find an effective and environmentally friendly control strategy to reduce the occurrence of RSRD and improve the rice yield.

Results: In pot experiments, the effect of B3 alone was better than that of fungicide or combined measures. The results showed that root colonization by B3 significantly reduced the incidence and disease index of RSRD by 41.0% and 53.8%, respectively. This was related to enhanced superoxide dismutase (SOD), peroxidase (POD), and polyphenol oxidase (PPO) activity, and to significantly upregulated expression levels of OsAOX, OsLOX, OsPAL, and OsPR10 in rice. Moreover, B3 improved the diversity of the bacterial community rather than the fungal community in the rice rhizosphere. It also led to a decrease in Fusarium proliferatum colonization and fumonisin content in the grain. Finally, root development was markedly promoted after B3 inoculation, and the yield improved by 48.60%. The result of field experiments showed that the incidence of RSRD and the fumonisin content were observably reduced in rice receiving B3, by 24.41% and 37.87%, respectively.

Conclusion: The endophytic fungus Phomopsis liquidambaris B3 may become an effective tool to relieve rice spikelet rot disease. © 2020 Society of Chemical Industry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jsfa.11042DOI Listing
December 2020

Antibiotic resistance contamination in four Italian municipal solid waste landfills sites spanning 34 years.

Chemosphere 2021 Mar 5;266:129182. Epub 2020 Dec 5.

State Key Laboratory of Pollution Control and Resources Reuse, Tongji University, Shanghai, 200092, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, PR China. Electronic address:

Municipal solid waste landfill is now recognized as a significant reservoir of antibiotics and antibiotic resistance genes (ARGs). This study investigates the contamination of antibiotics resistance, in 10 leachate samples collected from four MSW landfills in north Italy spanning 34 years, including ARGs as well as mobile genetic element (MGEs). Antibiotics (0-434740 ng/L) and ARGs (5.56-6.85 × 10copies/μL leachate) were found in leachate. Abundances of the measured ARGs were found to be clustered into two groups with different changing tendencies with landfilling age in different landfills. Even though some antibiotics were banned or limited in Italy, they were found to still occur in landfills and drive the long-term contamination of ARGs indirectly, indicating the persistence of antibiotic resistance. What's more, the complexity of antibiotic resistance in leachate was found to synthetically relate to antibiotics, metals, microbes and MGEs presenting that Mn, SMX and EFC influence positively (p < 0.01) the contamination of tetW, tetQ, tetM, tetA, ermB, and cat, contributing importantly in new leachate. This study discusses the AR pollution of leachate in Italy where antibiotics are used the most in Europe, less reported in literatures. Our results suggest that a full-scale view for landfill antibiotics resistance should be considered with history of landfills, use of antibiotics and different phase in landfills, with both "relative static" and "dynamic tracking" perspective to focus on the principal antibiotic-resistance pollutants for leachate treatment, and raise the attention for landfill post-closure care and landfill mining.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2020.129182DOI Listing
March 2021

Point-of-care testing (POCT) of patients with a high concentration of uric acid by using alginate hydrogel microspheres embedded with CdZnTeS QDs and urate oxidase (Alg@QDs-UOx MSs).

Analyst 2021 Feb 27;146(3):949-955. Epub 2020 Nov 27.

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.

High concentration of uric acid is usually related to cardiovascular and cerebrovascular diseases. Developing a simple method for the rapid and efficient detection of uric acid has a great significance in clinical diagnosis. In this work, alginate hydrogel microspheres embedded with CdZnTeS QDs and urate oxidase (Alg@QDs-UOx MSs) were prepared for the first time, and further used for point-of-care testing (POCT) of patients with a high concentration of uric acid. This strategy is mainly based on visual detection of HO, the product of uric acid after an enzymatic reaction. The proposed sensor (Alg@QDs-UOx MSs) has several advantages. First, it can reduce the interference of the proteins to the fluorescence of QDs. Second, Alg@QDs-UOx MSs help improve the stability of the CdZnTeS QDs as well as the activity of urate oxidase during storage. Third, it is easy to use, has fast response speed, and is of low cost. Therefore, the proposed sensor shows good application prospects. Simply through the built-in camera of a smartphone, we can visualize the urine samples from patients with a high concentration of uric acid within 10 minutes, and the accuracy rates were 100%. In the range of 100.0 μM to 900.0 μM, the I/I values and uric acid concentrations are in a great linear relationship (R = 0.9973), indicating that this method can be employed for quantitative analysis of uric acid in human urine (<10 mM). The limit of detection (LOD) is 20.3 μM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0an02029jDOI Listing
February 2021

Biological denitrification potential as an indicator for measuring digestate stability.

Sci Total Environ 2021 Jan 7;752:142211. Epub 2020 Sep 7.

State Key Laboratory of Pollution Control & Resource Reuse, Tongji University, Shanghai 200092, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, PR China; Institute of Waste Treatment and Reclamation, Tongji University, Shanghai 200092, PR China. Electronic address:

Biological stability is an essential parameter for assessing the environmental impact from the land application of digestate as organic amendment. In this paper, a new indicator, biological denitrification potential (BDP), was developed for evaluating the biological stability of digestate. Digestate samples collected along the digestion process from a mesophilic anaerobic batch digester fed with food waste were investigated under different solid retention time. The value of BDP based on nitrate removal ranged from 176.3 to 48.3 mg-N/g-VS, corresponding well to the digestion time, and strongly correlated with total organic carbon content. Evolution trends similar to respiration index (RI) and biochemical methane potential (BMP) can be also observed for BDP, indicating that values presented of these stability indices decreased with the degree of digestate stabilization. The mass balance of the BDP process indicated that nitrate was mainly converted into N gas with mineralizing organic carbon from digestate, implying that biostability evaluated by BDP depends on carbon source and denitrification activity in digestate. The denitrifying bacteria Thiopseudomonas and Pseudomonas accounted for the majority of microorganisms. These findings of this study concluded that BDP can be an efficient indicator to assess the bio-stability of digestate planned for agricultural or land use. Compared with the existing biostability index, BDP has the additional advantage of no exogenous inoculum addition, homogenous test condition and possibility of shortening incubation time.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2020.142211DOI Listing
January 2021