Publications by authors named "Louis Scampavia"

63Publications

High-Throughput Screening for Drugs That Inhibit Papain-Like Protease in SARS-CoV-2.

Authors:
Emery Smith Meredith E Davis-Gardner Ruben D Garcia-Ordonez Tu-Trinh Nguyen Mitchell Hull Emily Chen Pierre Baillargeon Louis Scampavia Timothy Strutzenberg Patrick R Griffin Michael Farzan Timothy P Spicer

SLAS Discov 2020 12 10;25(10):1152-1161. Epub 2020 Oct 10.

Department of Molecular Medicine, Scripps Research Institute, Scripps Florida, Jupiter, FL, USA.

View Article and Find Full Text PDF
December 2020

Identification of Compounds That Promote Readthrough of Premature Termination Codons in the CFTR.

Authors:
Emery Smith Danijela Dukovski Justin Shumate Louis Scampavia John P Miller Timothy P Spicer

SLAS Discov 2020 Oct 5:2472555220962001. Epub 2020 Oct 5.

Department of Molecular Medicine, Scripps Florida, The Scripps Research Institute Molecular Screening Center, Jupiter, FL, USA.

View Article and Find Full Text PDF
October 2020

Controls the Maturation of Dendrites, Synaptic Function, and Network Activity in Developing Human Neurons.

Authors:
Nerea Llamosas Vineet Arora Ridhima Vij Murat Kilinc Lukasz Bijoch Camilo Rojas Adrian Reich BanuPriya Sridharan Erik Willems David R Piper Louis Scampavia Timothy P Spicer Courtney A Miller J Lloyd Holder Gavin Rumbaugh

J Neurosci 2020 10 4;40(41):7980-7994. Epub 2020 Sep 4.

Department of Neuroscience, Scripps Research, Jupiter, Florida 33458

View Article and Find Full Text PDF
October 2020

Identification of Small-Molecule Inhibitors of Neutral Ceramidase (nCDase) via Target-Based High-Throughput Screening.

Authors:
Yuka Otsuka Michael V Airola Yong-Mi Choi Nicolas Coant Justin Snider Chris Cariello Essa M Saied Christoph Arenz Thomas Bannister Ron Rahaim Yusuf A Hannun Justin Shumate Louis Scampavia John D Haley Timothy P Spicer

SLAS Discov 2021 Jan 31;26(1):113-121. Epub 2020 Jul 31.

Department of Molecular Medicine, Scripps Research, The Scripps Research Molecular Screening Center, Jupiter, FL, USA.

View Article and Find Full Text PDF
January 2021

Pharmacologic IRE1/XBP1s activation confers targeted ER proteostasis reprogramming.

Authors:
Julia M D Grandjean Aparajita Madhavan Lauren Cech Bryan O Seguinot Ryan J Paxman Emery Smith Louis Scampavia Evan T Powers Christina B Cooley Lars Plate Timothy P Spicer Jeffery W Kelly R Luke Wiseman

Nat Chem Biol 2020 10 20;16(10):1052-1061. Epub 2020 Jul 20.

Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA.

View Article and Find Full Text PDF
October 2020

Rescue of mutant gonadotropin-releasing hormone receptor function independent of cognate receptor activity.

Authors:
Emery Smith Jo Ann Janovick Thomas D Bannister Justin Shumate Vadivel Ganapathy Louis Scampavia Timothy P Spicer

Sci Rep 2020 06 29;10(1):10579. Epub 2020 Jun 29.

Department of Molecular Medicine, The Scripps Research Molecular Screening Center, Scripps Research Florida, 130 Scripps Way #1A1, Jupiter, FL, 33458, USA.

View Article and Find Full Text PDF
June 2020

An unbiased high-throughput drug screen reveals a potential therapeutic vulnerability in the most lethal molecular subtype of pancreatic cancer.

Authors:
Chun-Hao Pan Yuka Otsuka BanuPriya Sridharan Melissa Woo Cindy V Leiton Sruthi Babu Mariana Torrente Gonçalves Ryan R Kawalerski Ji Dong K Bai David K Chang Andrew V Biankin Louis Scampavia Timothy Spicer Luisa F Escobar-Hoyos Kenneth R Shroyer

Mol Oncol 2020 08 4;14(8):1800-1816. Epub 2020 Jul 4.

Department of Pathology, Renaissance School of Medicine, Stony Brook University, NY, USA.

View Article and Find Full Text PDF
August 2020

Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.

Authors:
Leiming Wang Chiang-Min Cheng Jun Qin Mafei Xu Chung-Yang Kao Jingjing Shi Erli You Wanchun Gong Laura Pedro Rosa Peter Chase Louis Scampavia Franck Madoux Timothy Spicer Peter Hodder H Eric Xu Sophia Y Tsai Ming-Jer Tsai

Sci Adv 2020 May 29;6(18):eaaz8031. Epub 2020 Apr 29.

Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.

View Article and Find Full Text PDF
May 2020

Applications for Open Source Microplate-Compatible Illumination Panels.

Authors:
Pierre Baillargeon Timothy P Spicer Louis Scampavia

J Vis Exp 2019 10 3(152). Epub 2019 Oct 3.

The Scripps Research Molecular Screening Center, Department of Molecular Medicine, Scripps Florida;

View Article and Find Full Text PDF
October 2019

A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons.

Authors:
BanuPriya Sridharan Christopher Hubbs Nerea Llamosas Murat Kilinc Fakhar U Singhera Erik Willems David R Piper Louis Scampavia Gavin Rumbaugh Timothy P Spicer

Sci Rep 2019 06 21;9(1):9000. Epub 2019 Jun 21.

The Scripps Research Molecular Screening Center, Department of Molecular Medicine, Scripps Research, Jupiter, Florida, 33458, USA.

View Article and Find Full Text PDF
June 2019

Automating a Magnetic 3D Spheroid Model Technology for High-Throughput Screening.

Authors:
Pierre Baillargeon Justin Shumate Shurong Hou Virneliz Fernandez-Vega Nicholas Marques Glauco Souza Jan Seldin Timothy P Spicer Louis Scampavia

SLAS Technol 2019 08 21;24(4):420-428. Epub 2019 Jun 21.

1 The Scripps Research Molecular Screening Center, Department of Molecular Medicine, Scripps Florida, Jupiter, FL, USA.

View Article and Find Full Text PDF
August 2019

Design of Microplate-Compatible Illumination Panels for a Semiautomated Benchtop Pipetting System.

Authors:
Pierre Baillargeon Kervin Coss-Flores Fakhar Singhera Justin Shumate Hannah Williams Lina DeLuca Timothy P Spicer Louis Scampavia

SLAS Technol 2019 08 30;24(4):399-407. Epub 2019 Jan 30.

1 The Scripps Research Molecular Screening Center, Department of Molecular Medicine, Scripps Florida, Jupiter, Florida, USA.

View Article and Find Full Text PDF
August 2019

The Scripps Molecular Screening Center and Translational Research Institute.

Authors:
Pierre Baillargeon Virneliz Fernandez-Vega Banu Priya Sridharan Steven Brown Patrick R Griffin Hugh Rosen Benjamin Cravatt Louis Scampavia Timothy P Spicer

SLAS Discov 2019 03 25;24(3):386-397. Epub 2019 Jan 25.

1 Department of Molecular Medicine, Scripps Research Florida, Jupiter, FL, USA.

View Article and Find Full Text PDF
March 2019

Discovery and Characterization of Two Classes of Selective Inhibitors of the Suppressor of the TCR Signaling Family of Proteins.

Authors:
Weijie Zhou Yue Yin Emery Smith Jacqueline Chou Justin Shumate Louis Scampavia Timothy P Spicer Nicholas Carpino Jarrod B French

ACS Infect Dis 2019 02 14;5(2):250-259. Epub 2018 Dec 14.

Department of Molecular Medicine , Scripps Research Molecular Screening Center, Scripps Research , 130 Scripps Way , Jupiter , Florida 33458 , United States.

View Article and Find Full Text PDF
February 2019

Identification of Antimalarial Inhibitors Using Late-Stage Gametocytes in a Phenotypic Live/Dead Assay.

Authors:
Timothy P Spicer Donald L Gardiner Frank J Schoenen Sudeshna Roy Patrick R Griffin Peter Chase Louis Scampavia Peter Hodder Katharine R Trenholme

SLAS Discov 2019 01 24;24(1):38-46. Epub 2018 Aug 24.

2 School of Medicine, University of Queensland, Herston, Queensland, Australia.

View Article and Find Full Text PDF
January 2019

Chemical validation and optimization of pharmacoperones targeting vasopressin type 2 receptor mutant.

Authors:
Jo Ann Janovick Timothy P Spicer Thomas D Bannister Emery Smith Vadivel Ganapathy Louis Scampavia

Biochem J 2018 09 25;475(18):2941-2953. Epub 2018 Sep 25.

Lead Identification Division, Translational Research Institute and Department of Molecular Medicine, Scripps Research Institute, Jupiter, FL 33458, U.S.A.

View Article and Find Full Text PDF
September 2018

Identification of Novel, Structurally Diverse, Small Molecule Modulators of GPR119.

Authors:
Ainhoa Nieto Virneliz Fernández-Vega Timothy P Spicer Emmanuel Sturchler Pramisha Adhikari Nicole Kennedy Sean Mandat Peter Chase Louis Scampavia Thomas Bannister Peter Hodder Patricia H McDonald

Assay Drug Dev Technol 2018 07;16(5):278-288

1 Department of Molecular Medicine, The Scripps Research Institute , Jupiter, Florida.

View Article and Find Full Text PDF
July 2018

A Cytotoxic Three-Dimensional-Spheroid, High-Throughput Assay Using Patient-Derived Glioma Stem Cells.

Authors:
Victor Quereda Shurong Hou Franck Madoux Louis Scampavia Timothy P Spicer Derek Duckett

SLAS Discov 2018 09 11;23(8):842-849. Epub 2018 May 11.

1 Department of Molecular Medicine, Scripps Florida, Jupiter, FL, USA.

View Article and Find Full Text PDF
September 2018

A novel three-dimensional high-throughput screening approach identifies inducers of a mutant KRAS selective lethal phenotype.

Authors:
Smitha Kota Shurong Hou William Guerrant Franck Madoux Scott Troutman Virneliz Fernandez-Vega Nina Alekseeva Neeharika Madala Louis Scampavia Joseph Kissil Timothy P Spicer

Oncogene 2018 08 10;37(32):4372-4384. Epub 2018 May 10.

Department of Molecular Medicine, The Scripps Research Institute, Florida, USA.

View Article and Find Full Text PDF
August 2018

Advanced Development of Primary Pancreatic Organoid Tumor Models for High-Throughput Phenotypic Drug Screening.

Authors:
Shurong Hou Hervé Tiriac Banu Priya Sridharan Louis Scampavia Franck Madoux Jan Seldin Glauco R Souza Donald Watson David Tuveson Timothy P Spicer

SLAS Discov 2018 07 19;23(6):574-584. Epub 2018 Apr 19.

1 The Scripps Research Institute Molecular Screening Center, Department of Molecular Medicine, Scripps Florida, Jupiter, FL, USA.

View Article and Find Full Text PDF
July 2018

A High-Throughput Time-Resolved Fluorescence Energy Transfer Assay to Screen for Modulators of RGS7/Gβ5/R7BP Complex.

Authors:
Brian S Muntean Dipak N Patil Franck Madoux James Fossetta Louis Scampavia Timothy P Spicer Kirill A Martemyanov

Assay Drug Dev Technol 2018 04;16(3):150-161

1 Department of Neuroscience, The Scripps Research Institute , Jupiter, Florida.

View Article and Find Full Text PDF
April 2018

IoT for Real-Time Measurement of High-Throughput Liquid Dispensing in Laboratory Environments.

Authors:
Justin Shumate Pierre Baillargeon Timothy P Spicer Louis Scampavia

SLAS Technol 2018 10 12;23(5):440-447. Epub 2018 Apr 12.

1 The Scripps Research Institute Molecular Screening Center and Department of Molecular Medicine, Scripps Florida, Jupiter, FL, USA.

View Article and Find Full Text PDF
October 2018

Dynamic changes during the treatment of pancreatic cancer.

Authors:
Robert A Wolff Andrea Wang-Gillam Hector Alvarez Hervé Tiriac Dannielle Engle Shurong Hou Abigail F Groff Anthony San Lucas Vincent Bernard Kelvin Allenson Jonathan Castillo Dong Kim Feven Mulu Jonathan Huang Bret Stephens Ignacio I Wistuba Matthew Katz Gauri Varadhachary YoungKyu Park James Hicks Arul Chinnaiyan Louis Scampavia Timothy Spicer Chiara Gerhardinger Anirban Maitra David Tuveson John Rinn Gregory Lizee Cassian Yee Arnold J Levine

Oncotarget 2018 Mar 13;9(19):14764-14790. Epub 2018 Feb 13.

Simons Center for Systems Biology, Institute for Advanced Study, Princeton, NJ, USA.

View Article and Find Full Text PDF
March 2018

Improved Scalability of Neuron-Based Phenotypic Screening Assays for Therapeutic Discovery in Neuropsychiatric Disorders.

Authors:
Timothy P Spicer Christopher Hubbs Thomas Vaissiere Deanna Collia Camilo Rojas Murat Kilinc Kyle Vick Franck Madoux Pierre Baillargeon Justin Shumate Kirill A Martemyanov Damon T Page Sathya Puthanveettil Peter Hodder Ronald Davis Courtney A Miller Louis Scampavia Gavin Rumbaugh

Mol Neuropsychiatry 2018 Feb 17;3(3):141-150. Epub 2017 Nov 17.

Department of Molecular Medicine, Jupiter, FL, USA.

View Article and Find Full Text PDF
February 2018

A Homogeneous Cell-Based Halide-Sensitive Yellow Fluorescence Protein Assay to Identify Modulators of the Cystic Fibrosis Transmembrane Conductance Regulator Ion Channel.

Authors:
Emery Smith Kenneth A Giuliano Justin Shumate Pierre Baillargeon Brigid McEwan Matthew D Cullen John P Miller Lawrence Drew Louis Scampavia Timothy P Spicer

Assay Drug Dev Technol 2017 Dec 27;15(8):395-406. Epub 2017 Nov 27.

1 Department of Molecular Medicine, The Scripps Research Institute Molecular Screening Center , Scripps Florida, Jupiter, Florida.

View Article and Find Full Text PDF
December 2017

Using bead injection to model dispensing of 3-D multicellular spheroids into microtiter plates.

Authors:
Fakhar Singhera Emily Cooper Louis Scampavia Timothy Spicer

Talanta 2018 Jan 9;177:74-76. Epub 2017 Sep 9.

The Scripps Research Institute Molecular Screening Center, Department of Molecular Medicine, Scripps Florida, 130 Scripps Way, Jupiter, FL, USA.

View Article and Find Full Text PDF
January 2018

High-Throughput Screening for Protein Synthesis Inhibitors Targeting Aminoacyl-tRNA Synthetases.

Authors:
Jiwon Kong Pengfei Fang Franck Madoux Timothy P Spicer Louis Scampavia Sunghoon Kim Min Guo

SLAS Discov 2018 02 11;23(2):174-182. Epub 2017 Oct 11.

2 Department of Cancer Biology, Scripps Research Institute, Scripps Florida, Jupiter, FL, USA.

View Article and Find Full Text PDF
February 2018

A Rapid Phenotypic Whole-Cell Screening Approach for the Identification of Small-Molecule Inhibitors That Counter β-Lactamase Resistance in Pseudomonas aeruginosa.

Authors:
Deanna Collia Thomas D Bannister Hao Tan Shouguang Jin Taimour Langaee Justin Shumate Louis Scampavia Timothy P Spicer

SLAS Discov 2018 01 29;23(1):55-64. Epub 2017 Aug 29.

1 The Scripps Research Institute Molecular Screening Center, Department of Molecular Medicine, Scripps Florida, Jupiter, FL, USA.

View Article and Find Full Text PDF
January 2018

Pharmacoperone rescue of vasopressin 2 receptor mutants reveals unexpected constitutive activity and coupling bias.

Authors:
Jo Ann Janovick Timothy P Spicer Thomas D Bannister Louis Scampavia P Michael Conn

PLoS One 2017 2;12(8):e0181830. Epub 2017 Aug 2.

Departments of Internal Medicine and Cell Biology/Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas, United States of America.

View Article and Find Full Text PDF
October 2017

Discovery of an enzyme and substrate selective inhibitor of ADAM10 using an exosite-binding glycosylated substrate.

Authors:
Franck Madoux Daniela Dreymuller Jean-Phillipe Pettiloud Radleigh Santos Christoph Becker-Pauly Andreas Ludwig Gregg B Fields Thomas Bannister Timothy P Spicer Mare Cudic Louis D Scampavia Dmitriy Minond

Sci Rep 2016 Dec 5;6(1):11. Epub 2016 Dec 5.

Department of Drug Discovery and Development, Harrison School of Pharmacy, 3211B Walker Building, Auburn University, Auburn, Alabama, 36849, USA.

View Article and Find Full Text PDF
December 2016

Drug Library Screening for the Identification of Ionophores That Correct the Mistrafficking Disorder Associated with Oxalosis Kidney Disease.

Authors:
Shurong Hou Franck Madoux Louis Scampavia Jo Ann Janovick P Michael Conn Timothy P Spicer

SLAS Discov 2017 08 31;22(7):887-896. Epub 2017 Jan 31.

1 Department of Molecular Therapeutics, Scripps Research Institute Molecular Screening Center, Scripps Research Institute, Jupiter, FL, USA.

View Article and Find Full Text PDF
August 2017

A 1536-Well 3D Viability Assay to Assess the Cytotoxic Effect of Drugs on Spheroids.

Authors:
Franck Madoux Allison Tanner Michelle Vessels Lynsey Willetts Shurong Hou Louis Scampavia Timothy P Spicer

SLAS Discov 2017 06 13;22(5):516-524. Epub 2017 Jan 13.

1 Department of Molecular Medicine, Scripps Florida, Jupiter, FL, USA.

View Article and Find Full Text PDF
June 2017

Development of an HTS-Compatible Assay for Discovery of Melanoma-Related Microphthalmia Transcription Factor Disruptors Using AlphaScreen Technology.

Authors:
Jing Wang Pengfei Fang Peter Chase Sagi Tshori Ehud Razin Timothy P Spicer Louis Scampavia Peter Hodder Min Guo

SLAS Discov 2017 01 11;22(1):58-66. Epub 2016 Nov 11.

1 Department of Cancer Biology, The Scripps Research Institute, Scripps Florida, Jupiter, FL, USA.

View Article and Find Full Text PDF
January 2017

Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation.

Authors:
Lars Plate Christina B Cooley John J Chen Ryan J Paxman Ciara M Gallagher Franck Madoux Joseph C Genereux Wesley Dobbs Dan Garza Timothy P Spicer Louis Scampavia Steven J Brown Hugh Rosen Evan T Powers Peter Walter Peter Hodder R Luke Wiseman Jeffery W Kelly

Elife 2016 07 20;5. Epub 2016 Jul 20.

Department of Chemistry, The Scripps Research Institute, La Jolla, United States.

View Article and Find Full Text PDF
July 2016

Receptor antagonism/agonism can be uncoupled from pharmacoperone activity.

Authors:
Jo Ann Janovick Timothy P Spicer Emery Smith Thomas D Bannister Terry Kenakin Louis Scampavia P Michael Conn

Mol Cell Endocrinol 2016 10 4;434:176-85. Epub 2016 Jul 4.

Departments of Internal Medicine and Cell Biology/Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX, United States. Electronic address:

View Article and Find Full Text PDF
October 2016

Identification of Potential Pharmacoperones Capable of Rescuing the Functionality of Misfolded Vasopressin 2 Receptor Involved in Nephrogenic Diabetes Insipidus.

Authors:
Emery Smith Jo Ann Janovick Thomas D Bannister Justin Shumate Louis Scampavia P Michael Conn Timothy P Spicer

J Biomol Screen 2016 Sep 8;21(8):824-31. Epub 2016 Jun 8.

The Scripps Research Institute Molecular Screening Center, Department of Molecular Therapeutics, Scripps Florida, Jupiter, FL, USA

View Article and Find Full Text PDF
September 2016

Fluorometric High-Throughput Screening Assay for Secreted Phospholipases A2 Using Phospholipid Vesicles.

Authors:
Heather Ewing Virneliz Fernández-Vega Timothy P Spicer Peter Chase Steven Brown Louis Scampavia William R Roush Sean Riley Hugh Rosen Peter Hodder Gerard Lambeau Michael H Gelb

J Biomol Screen 2016 Aug 4;21(7):713-21. Epub 2016 May 4.

Department of Chemistry, University of Washington, Seattle, WA, USA

View Article and Find Full Text PDF
August 2016

An Integrated Approach for Screening and Identification of Positive Allosteric Modulators of N-Methyl-D-Aspartate Receptors.

Authors:
Enrique Jambrina Rok Cerne Emery Smith Louis Scampavia Maria Cuadrado Jeremy Findlay Michael J Krambis Mark Wakulchik Peter Chase Michael Brunavs Kevin D Burris Peter Gallagher Timothy P Spicer Daniel Ursu

J Biomol Screen 2016 Jun 2;21(5):468-79. Epub 2016 Feb 2.

Eli Lilly & Company Ltd., Windlesham, UK

View Article and Find Full Text PDF
June 2016

Ebselen, a Small-Molecule Capsid Inhibitor of HIV-1 Replication.

Authors:
Suzie Thenin-Houssier Ian Mitchelle S de Vera Laura Pedro-Rosa Angela Brady Audrey Richard Briana Konnick Silvana Opp Cindy Buffone Jakob Fuhrmann Smitha Kota Blase Billack Magdalena Pietka-Ottlik Timothy Tellinghuisen Hyeryun Choe Timothy Spicer Louis Scampavia Felipe Diaz-Griffero Douglas J Kojetin Susana T Valente

Antimicrob Agents Chemother 2016 Apr 25;60(4):2195-208. Epub 2016 Mar 25.

Department of Immunology and Microbial Sciences, The Scripps Research Institute, Jupiter, Florida, USA

View Article and Find Full Text PDF
April 2016

An Automated Miniaturized Method to Perform and Analyze Antimicrobial Drug Synergy Assays.

Authors:
Peter Chase Imarhia Enogieru Franck Madoux Eric Bishop Jacob Beer Louis Scampavia Timothy Spicer

Assay Drug Dev Technol 2016 Jan-Feb;14(1):58-66. Epub 2015 Dec 15.

Lead Identification, Department of Molecular Therapeutics, The Scripps Research Institute , Scripps Florida, Jupiter, Florida.

View Article and Find Full Text PDF
December 2016

First-in-Class Inhibitors of Sulfur Metabolism with Bactericidal Activity against Non-Replicating M. tuberculosis.

Authors:
Prakash B Palde Ashima Bhaskar Laura E Pedró Rosa Franck Madoux Peter Chase Vinayak Gupta Timothy Spicer Louis Scampavia Amit Singh Kate S Carroll

ACS Chem Biol 2016 Jan 11;11(1):172-84. Epub 2015 Nov 11.

Department of Chemistry, The Scripps Research Institute , Jupiter, Florida 33458, United States.

View Article and Find Full Text PDF
January 2016

Assay strategies for identification of therapeutic leads that target protein trafficking.

Authors:
P Michael Conn Timothy P Spicer Louis Scampavia Jo Ann Janovick

Trends Pharmacol Sci 2015 Aug 8;36(8):498-505. Epub 2015 Jun 8.

Department of Internal Medicine, Center for Membrane Protein Research, Texas Tech University Health Sciences Center, Lubbock, TX, USA; Department of Cell Biology/Biochemistry, Center for Membrane Protein Research, Texas Tech University Health Sciences Center, Lubbock, TX, USA.

View Article and Find Full Text PDF
August 2015

Application of Parallel Multiparametric Cell-Based FLIPR Detection Assays for the Identification of Modulators of the Muscarinic Acetylcholine Receptor 4 (M4).

Authors:
Emery Smith Peter Chase Colleen M Niswender Thomas J Utley Douglas J Sheffler Meredith J Noetzel Atin Lamsal Michael R Wood P Jeffrey Conn Craig W Lindsley Franck Madoux Mary Acosta Louis Scampavia Timothy Spicer Peter Hodder

J Biomol Screen 2015 Aug 15;20(7):858-68. Epub 2015 Apr 15.

The Scripps Research Institute Molecular Screening Center, Scripps Florida, Jupiter, FL, USA Amgen Inc., Thousand Oaks, CA, USA.

View Article and Find Full Text PDF
August 2015

Identification of small molecules that disrupt signaling between ABL and its positive regulator RIN1.

Authors:
Pamela Y Ting Robert Damoiseaux Björn Titz Kenneth A Bradley Thomas G Graeber Virneliz Fernández-Vega Thomas D Bannister Peter Chase Reji Nair Louis Scampavia Peter Hodder Timothy P Spicer John Colicelli

PLoS One 2015 26;10(3):e0121833. Epub 2015 Mar 26.

Molecular Biology Institute, Jonsson Comprehensive Cancer Center, Department of Biological Chemistry, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America.

View Article and Find Full Text PDF
February 2016

Development of a phenotypic high-content assay to identify pharmacoperone drugs for the treatment of primary hyperoxaluria type 1 by high-throughput screening.

Authors:
Franck Madoux Jo Ann Janovick David Smithson Sonia Fargue Christopher J Danpure Louis Scampavia Yih-Tai Chen Timothy P Spicer P Michael Conn

Assay Drug Dev Technol 2015 Jan-Feb;13(1):16-24

1 Lead Identification Division, Translational Research Institute, Scripps Research Institute , Jupiter, Florida.

View Article and Find Full Text PDF
November 2015

Selective inhibitor of platelet-activating factor acetylhydrolases 1b2 and 1b3 that impairs cancer cell survival.

Authors:
Jae Won Chang Andrea M Zuhl Anna E Speers Sherry Niessen Steven J Brown Melinda M Mulvihill Yi Chiao Fan Timothy P Spicer Mark Southern Louis Scampavia Virneliz Fernandez-Vega Melissa M Dix Michael D Cameron Peter S Hodder Hugh Rosen Daniel K Nomura Ohyun Kwon Ku-Lung Hsu Benjamin F Cravatt

ACS Chem Biol 2015 Apr 20;10(4):925-32. Epub 2015 Jan 20.

#Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569, United States.

View Article and Find Full Text PDF
April 2015

Identification of potent inhibitors of the Trypanosoma brucei methionyl-tRNA synthetase via high-throughput orthogonal screening.

Authors:
Laura Pedró-Rosa Frederick S Buckner Ranae M Ranade Christina Eberhart Franck Madoux J Robert Gillespie Cho Yeow Koh Steven Brown Jacqueline Lohse Christophe L M Verlinde Erkang Fan Thomas Bannister Louis Scampavia Wim G J Hol Timothy Spicer Peter Hodder

J Biomol Screen 2015 Jan 27;20(1):122-30. Epub 2014 Aug 27.

The Scripps Research Institute Molecular Screening Center, Scripps Florida, Jupiter, FL, USA Amgen, Inc., Thousand Oaks, CA.

View Article and Find Full Text PDF
January 2015

Development of high throughput screening assays and pilot screen for inhibitors of metalloproteases meprin α and β.

Authors:
Franck Madoux Claudia Tredup Timothy P Spicer Louis Scampavia Peter S Chase Peter S Hodder Gregg B Fields Christoph Becker-Pauly Dmitriy Minond

Biopolymers 2014 Sep;102(5):396-406

Lead Identification Division, Translational Research Institute, The Scripps Research Institute, 130 Scripps Way, Jupiter, Fl, 34987.

View Article and Find Full Text PDF
September 2014

A phenotypic high throughput screening assay for the identification of pharmacoperones for the gonadotropin releasing hormone receptor.

Authors:
P Michael Conn Emery Smith Timothy Spicer Peter Chase Louis Scampavia Jo Ann Janovick

Assay Drug Dev Technol 2014 May;12(4):238-46

1 Departments of Internal Medicine and Cell Biology/Biochemistry, Texas Tech University Health Sciences Center , Lubbock, Texas.

View Article and Find Full Text PDF
May 2014

Identification of Potent and Selective Inhibitors of the Plasmodium falciparum M18 Aspartyl Aminopeptidase (PfM18AAP) of Human Malaria via High-Throughput Screening.

Authors:
Timothy Spicer Virneliz Fernandez-Vega Peter Chase Louis Scampavia Joyce To John P Dalton Fabio L Da Silva Tina S Skinner-Adams Donald L Gardiner Katharine R Trenholme Christopher L Brown Partha Ghosh Patrick Porubsky Jenna L Wang David A Whipple Frank J Schoenen Peter Hodder

J Biomol Screen 2014 Aug 11;19(7):1107-15. Epub 2014 Mar 11.

The Scripps Research Institute Molecular Screening Center, Scripps Florida, Jupiter, FL, USA

View Article and Find Full Text PDF
August 2014

Monitoring of HTS compound library quality via a high-resolution image acquisition and processing instrument.

Authors:
Pierre Baillargeon Louis Scampavia Ross Einsteder Peter Hodder

J Lab Autom 2011 Jun 16;16(3):197-203. Epub 2011 May 16.

Lead Identification Division, Translational Research Institute, Scripps Research Institute Molecular Screening Center, Jupiter, FL 33458, USA.

View Article and Find Full Text PDF
June 2011

A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerization.

Authors:
Smitha Kota Louis Scampavia Timothy Spicer Aaron B Beeler Virginia Takahashi John K Snyder John A Porco Peter Hodder Arthur Donny Strosberg

Assay Drug Dev Technol 2010 Feb;8(1):96-105

Department of Infectology, The Scripps Research Institute-Scripps Florida, Jupiter, Florida 33458, USA.

View Article and Find Full Text PDF
February 2010

Comparison of miniaturized time-resolved fluorescence resonance energy transfer and enzyme-coupled luciferase high-throughput screening assays to discover inhibitors of Rho-kinase II (ROCK-II).

Authors:
Thomas Schröter Dmitriy Minond Amiee Weiser Chinh Dao Jeff Habel Timothy Spicer Peter Chase Pierre Baillargeon Louis Scampavia Stephan Schürer Caty Chung Chris Mader Mark Southern Nick Tsinoremas Philip LoGrasso Peter Hodder

J Biomol Screen 2008 Jan;13(1):17-28

Department of Molecular Therapeutics and Drug Discovery, Scripps Florida, Jupiter, Florida 33458, USA.

View Article and Find Full Text PDF
January 2008

Automated method, based on micro-sequential injection, for the study of enzyme kinetics and inhibition.

Authors:
Yan Chen Andrea D Carroll Louis Scampavia Jaromir Ruzicka

Anal Sci 2006 Jan;22(1):9-14

Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, USA.

View Article and Find Full Text PDF
January 2006

On-line flow cytometry for real-time surgical guidance.

Authors:
Ali H Mesiwala Louis D Scampavia Peter S Rabinovitch Jaromir Ruzicka Robert C Rostomily

Neurosurgery 2004 Sep;55(3):551-60; discussion 560-1

Department of Neurological Surgery, University of Washington, Seattle, Washington, USA.

View Article and Find Full Text PDF
September 2004

Automated affinity chromatography measurements of compound mixtures using a lab-on-valve apparatus coupled to electrospray ionization mass spectrometry.

Authors:
Yuko Ogata Louis Scampavia Tyan L Carter Erkang Fan Frantisek Turecek

Anal Biochem 2004 Aug;331(1):161-8

Department of Chemistry, University of Washington, Seattle, WA 98195, USA.

View Article and Find Full Text PDF
August 2004

A novel approach for monitoring extracellular acidification rates: based on bead injection spectrophotometry and the lab-on-valve system.

Authors:
Holger A Erxleben Michael K Manion David M Hockenbery Louis Scampavia Jaromir Ruzicka

Analyst 2004 Mar 2;129(3):205-12. Epub 2004 Feb 2.

Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, USA.

View Article and Find Full Text PDF
March 2004

Bead injection ELISA for the determination of antibodies implicated in type 1 diabetes mellitus.

Authors:
Andrea D Carroll Louis Scampavia Dong Luo Ake Lernmark Jaromir Ruzicka

Analyst 2003 Sep;128(9):1157-62

Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, USA.

View Article and Find Full Text PDF
September 2003

Micro sequential injection: automated insulin derivatization and separation using a lab-on-valve capillary electrophoresis system.

Authors:
Chao-Hsiang Wu Louis Scampavia Jaromir Ruzicka

Analyst 2003 Sep;128(9):1123-30

Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, USA.

View Article and Find Full Text PDF
September 2003

Real-time monitoring of lactate extrusion and glucose consumption of cultured cells using a lab-on-valve system.

Authors:
Craig M Schulz Louis Scampavia Jaromir Ruzicka

Analyst 2002 Dec;127(12):1583-8

University of Washington, Department of Chemistry, PO Box 351700, Seattle, WA 98195, USA.

View Article and Find Full Text PDF
December 2002

Label dilution method: a novel tool for bioligand interaction studies using bead injection in the lab-on-valve format.

Authors:
Andrea D Carroll Louis Scampavia Jaromir Ruzicka

Analyst 2002 Sep;127(9):1228-32

Department of Chemistry, University of Washington, Seattle 98195, USA.

View Article and Find Full Text PDF
September 2002

Automated affinity capture-release of biotin-containing conjugates using a lab-on-valve apparatus coupled to UV/visible and electrospray ionization mass spectrometry.

Authors:
Yuko Ogata Louis Scampavia Jaromír Růzicka C Ronald Scott Michael H Gelb Frantisek Turecek

Anal Chem 2002 Sep;74(18):4702-8

Department of Chemistry, University of Washington, Seattle 98195-1700, USA.

View Article and Find Full Text PDF
September 2002

Microsequential injection: anion separations using 'lab-on-valve' coupled with capillary electrophoresis.

Authors:
Chao-Hsiang Wu Louis Scampavia Jaromir Ruzicka

Analyst 2002 Jul;127(7):898-905

Department of Chemistry, University of Washington, Seattle 98195-1700, USA.

View Article and Find Full Text PDF
July 2002