Publications by authors named "Longfei Wu"

72 Publications

Low-dose tirofiban is associated with reduced in-hospital mortality in cardioembolic stroke patients treated with endovascular thrombectomy.

J Neurol Sci 2021 Aug 15;427:117539. Epub 2021 Jun 15.

Beijing Institute for Brain Disorders, Capital Medical University, Beijing, China; Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China. Electronic address:

Background And Purpose: Whether tirofiban is safe and effective in cardioembolic stroke patients treated with endovascular thrombectomy (EVT) remains unknown; this study evaluated the safety and efficacy of low-dose tirofiban in this patients population.

Methods: This study was a prospective registry study. Patients with cardioembolic stroke undergoing EVT from January 2013 to December 2020 were treated with EVT alone or EVT plus low-dose tirofiban. The primary outcome was symptomatic intracerebral hemorrhage (sICH) prior to discharge. The secondary outcomes included reocclusion, in-hospital mortality, and 3-month functional outcomes.

Results: Overall, 288 patients were recruited and 117 received low-dose tirofiban; 137 patients (47.6%) experienced ICH, 42 patients (14.6%) were sICH, and 23 patients (8%) were fatal ICH. Thirteen patients (11.1%) receiving tirofiban and 29 patients (17.0%) not receiving tirofiban experienced sICH (p = 0.167). Reocclusion occurred in nine patients (7.7%) receiving tirofiban and 15 patients (8.8%) not receiving tirofiban (p = 0.745). The rates of hernia (6.8% versus 20.5%) and decompressive craniectomy (2.6% versus 11.7%) were significantly lower in patients receiving tirofiban (p < 0.01). At 3-month follow-up, functional independence was achieved in 39 patients(33.3%) receiving tirofiban and 43 patients (25.1%) not receiving tirofiban (p = 0.131). Tirofiban was associated with lower odds of in-hospital mortality (3.4% versus 12.3%; adjusted odds ratio, 0.16; 95% confidence interval, 0.03-0.81; adjusted p = 0.027).

Conclusions: In patients with cardioembolic stroke undergoing EVT, tirofiban is not associated with higher sICH, it seems to lead to lower odds of in-hospital death. Further investigations are needed to confirm these results and to determine the optimal treatment protocols of tirofiban.
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http://dx.doi.org/10.1016/j.jns.2021.117539DOI Listing
August 2021

Mapping QTL associated with partial resistance to Aphanomyces root rot in pea (Pisum sativum L.) using a 13.2 K SNP array and SSR markers.

Theor Appl Genet 2021 Jun 15. Epub 2021 Jun 15.

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada.

Key Message: A stable and major QTL, which mapped to an approximately 20.0 cM region on pea chromosome 4, was identified as the most consistent region conferring partial resistance to Aphanomyces euteiches. Aphanomyces root rot (ARR), caused by Aphanomyces euteiches Drechs., is a destructive soilborne disease of field pea (Pisum Sativum L.). No completely resistant pea germplasm is available, and current ARR management strategies rely on partial resistance and fungicidal seed treatments. In this study, an F recombinant inbred line population of 135 individuals from the cross 'Reward' (susceptible) × '00-2067' (tolerant) was evaluated for reaction to ARR under greenhouse conditions with the A. euteiches isolate Ae-MDCR1 and over 2 years in a field nursery in Morden, Manitoba. Root rot severity, foliar weight, plant vigor and height were used as estimates of tolerance to ARR. Genotyping was conducted with a 13.2 K single-nucleotide polymorphism (SNP) array and 222 simple sequence repeat (SSR) markers. Statistical analyses of the phenotypic data indicated significant (P < 0.001) genotypic effects and significant G × E interactions (P < 0.05) in all experiments. After filtering, 3050 (23.1%) of the SNP and 30 (13.5%) of the SSR markers were retained for linkage analysis, which distributed 2999 (2978 SNP + 21 SSR) of the markers onto nine linkage groups representing the seven chromosomes of pea. Mapping of quantitative trait loci (QTL) identified 8 major-effect (R > 20%), 13 moderate-effect (10% < R < 20%) effect and 6 minor-effect (R < 10%) QTL. A genomic region on chromosome 4, delimited by the SNP markers PsCam037549_22628_1642 and PsCam026054_14999_2864, was identified as the most consistent region responsible for partial resistance to A. euteiches isolate Ae-MDCR1. Other genomic regions important for resistance were of the order chromosome 5, 6 and 7.
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http://dx.doi.org/10.1007/s00122-021-03871-6DOI Listing
June 2021

Efficacy and safety of normobaric hyperoxia combined with intravenous thrombolysis on acute ischemic stroke patients.

Neurol Res 2021 Jun 15:1-6. Epub 2021 Jun 15.

Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

Intravenous thrombolysis elevates the prognostic level of acute ischemic stroke (AIS) patients. Normobaric hyperoxia (NBO) delays the progression of the infarct core and promotes neurological recovery. However, it is uncertain whether NBO can further raise the prognostic level of AIS patients based on intravenous thrombolysis. To explore the efficacy and safety of NBO combined with intravenous thrombolysis on AIS patients. This observational study included anterior circulation stroke patients who received intravenous thrombolysis within 4.5 h after stroke onset. These patients were divided into two groups based on whether or not they received NBO therapy. The baseline data and the prognosis of the two groups were compared. The primary outcome was the proportion of functional independence (modified Rankin Scale 0-2) at 90 days post discharge. A total of 227 patients were included in this study. 125 patients received NBO therapy combined with intravenous thrombolysis, while 102 patients received intravenous thrombolysis only. Overall, the rate of recanalization was 83.3%. Consequently, 101 patients (80.8%) who received NBO combined with intravenous thrombolysis and 63 patients (61.8%) in the control group achieved functional independence (P = 0.002). Multivariable logistic regression analysis showed that NBO combined with intravenous thrombolysis over intravenous thrombolysis alone was associated with 90-day functional independence (OR: 2.318; 95% CI: 1.226-4.381; P = 0.01). This study verified the efficacy and safety of NBO combined with intravenous thrombolysis in AIS patients. Prospective study is needed to further substantiate these findings.
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http://dx.doi.org/10.1080/01616412.2021.1939234DOI Listing
June 2021

Sub-Second Time-Resolved Surface-Enhanced Raman Spectroscopy Reveals Dynamic CO Intermediates during Electrochemical CO Reduction on Copper.

Angew Chem Int Ed Engl 2021 Jul 15;60(30):16576-16584. Epub 2021 Jun 15.

Inorganic Chemistry and Catalysis, Institute for Sustainable and Circular Chemistry, Utrecht University, Universiteitsweg 99, 3584, CG, Utrecht, The Netherlands.

The electrocatalytic carbon dioxide (CO ) reduction reaction (CO RR) into hydrocarbons is a promising approach for greenhouse gas mitigation, but many details of this dynamic reaction remain elusive. Here, time-resolved surface-enhanced Raman spectroscopy (TR-SERS) is employed to successfully monitor the dynamics of CO RR intermediates and Cu surfaces with sub-second time resolution. Anodic treatment at 1.55 V vs. RHE and subsequent surface oxide reduction (below -0.4 V vs. RHE) induced roughening of the Cu electrode surface, which resulted in hotspots for TR-SERS, enhanced time resolution (down to ≈0.7 s) and fourfold improved CO RR efficiency toward ethylene. With TR-SERS, the initial restructuring of the Cu surface was followed (<7 s), after which a stable surface surrounded by increased local alkalinity was formed. Our measurements revealed that a highly dynamic CO intermediate, with a characteristic vibration below 2060 cm , is related to C-C coupling and ethylene production (-0.9 V vs. RHE), whereas lower cathodic bias (-0.7 V vs. RHE) resulted in gaseous CO production from isolated and static CO surface species with a distinct vibration at 2092 cm .
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http://dx.doi.org/10.1002/anie.202104114DOI Listing
July 2021

Outcomes in Endovascular Therapy for Basilar Artery Occlusion: Intracranial Atherosclerotic Disease . Embolism.

Aging Dis 2021 Apr 1;12(2):404-414. Epub 2021 Apr 1.

8Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

Acute ischemic stroke due to basilar artery occlusion (BAO) carries a very poor prognosis. Functional outcomes in BAO patients undergoing endovascular therapy (EVT) may differ according to the specific pathological mechanisms. We aimed to explore the impact of the underlying pathological mechanisms on prognosis at 90-days and long-term follow-up in BAO patients treated with EVT. We analyzed consecutive BAO patients undergoing EVT from December 2012 to December 2018 at a single center (Xuanwu Hospital). Patients were classified into either an intracranial atherosclerotic disease (ICAD) group or an embolic group according to the corresponding angiographic findings. The baseline characteristics and functional outcomes were compared between the two groups. Multivariable logistic regression analysis was performed. Among the 167 patients enrolled, 78 patients (46.7%) were in the ICAD group and 89 patients (53.3%) were assigned to the embolic group. Overall, 149 patients (89.2%) achieved successful reperfusion post-EVT. There were no significant differences in functional outcomes at 90-days and long-term follow-up between the two groups. Similarly, a Kaplan-Meier survival analysis showed similar long-term survival probabilities (P = 0.438). The pathological mechanism was not associated with functional independence (OR, 1.818; 95% CI, 0.694-4.761; P = 0.224), favorable outcome (OR, 1.476; 95% CI, 0.592-3.681; P = 0.403), or mortality (OR, 1.249; 95% CI, 0.483-3.226; P = 0.646). However, based on subgroup analysis, embolic BAO versus ICAD was significantly associated with better functional independence in those aged 60 years and younger (OR, 4.513; 95% CI, 1.138-17.902). In this study, no differences in either 90-days or long-term functional outcomes between ICAD-related BAO and embolic BAO patients undergoing EVT were observed. However, in BAO patients aged ≤ 60 years, the pathological mechanism of embolism was associated with better functional independence.
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http://dx.doi.org/10.14336/AD.2020.0704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990363PMC
April 2021

SDL Index Predicts Stroke-Associated Pneumonia in Patients After Endovascular Therapy.

Front Neurol 2021 16;12:622272. Epub 2021 Feb 16.

Department of Neurosurgery, Xuanwu Hospital of Capital Medical University, Beijing, China.

This study aimed to develop and validate a novel index to predict SAP for AIS patients who underwent endovascular treatment. A study was conducted in an advanced comprehensive stroke center from January 2013 to December 2019 aiming to develop and validate a novel index to predict SAP for AIS patients who underwent endovascular treatment. This cohort consisted of a total of 407 consecutively registered AIS patients who underwent endovascular therapy, which was divided into derivation and validation cohorts. Multiple blood parameters as well as demographic features, vascular risk factors, and clinical features were carefully evaluated in the derivation cohort. The independent predictors were obtained using multivariable logistic regression. The scoring system was generated based on the β-coefficients of each independent risk factor. Ultimately, a novel predictive model: the SDL index (stroke history, dysphagia, lymphocyte count < 1.00 × 10/μL) was developed. The SDL index showed good discrimination both in the derivation cohort (AUROC: 0.739, 95% confidence interval, 0.678-0.801) and the validation cohort (AUROC: 0.783, 95% confidence interval, 0.707-0.859). The SDL index was well-calibrated (Hosmer-Lemeshow test) in the derivation cohort ( = 0.389) and the validation cohort ( = 0.692). We therefore divided our population into low (SDL index = 0), medium (SDL index = 1), and high (SDL index ≥ 2) risk groups for SAP. The SDL index showed good discrimination when compared with two existing SAP prediction models. The SDL index is a novel feasible tool to predict SAP risk in acute ischemic stroke patients post endovascular treatment.
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http://dx.doi.org/10.3389/fneur.2021.622272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921145PMC
February 2021

Stabilization effects in binary colloidal Cu and Ag nanoparticle electrodes under electrochemical CO reduction conditions.

Nanoscale 2021 Mar;13(9):4835-4844

Inorganic Chemistry and Catalysis group, Institute for Sustainable and Circular Chemistry, Utrecht University, 3584 CG Utrecht, The Netherlands.

Nanoparticle modified electrodes constitute an attractive way to tailor-make efficient carbon dioxide (CO2) reduction catalysts. However, the restructuring and sintering processes of nanoparticles under electrochemical reaction conditions not only impedes the widespread application of nanoparticle catalysts, but also misleads the interpretation of the selectivity of the nanocatalysts. Here, we colloidally synthesized metallic copper (Cu) and silver (Ag) nanoparticles with a narrow size distribution (<10%) and utilized them in electrochemical CO2 reduction reactions. Monometallic Cu and Ag nanoparticle electrodes showed severe nanoparticle sintering already at low overpotential of -0.8 V vs. RHE, as evidenced by ex situ SEM investigations, and potential-dependent variations in product selectivity that resemble bulk Cu (14% for ethylene at -1.3 V vs. RHE) and Ag (69% for carbon monoxide at -1.0 V vs. RHE). However, by co-deposition of Cu and Ag nanoparticles, a nanoparticle stabilization effect was observed between Cu and Ag, and the sintering process was greatly suppressed at CO2 reducing potentials (-0.8 V vs. RHE). Furthermore, by varying the Cu/Ag nanoparticle ratio, the CO2 reduction reaction (CO2RR) selectivity towards methane (maximum of 20.6% for dense Cu2.5-Ag1 electrodes) and C2 products (maximum of 15.7% for dense Cu1-Ag1 electrodes) can be tuned, which is attributed to a synergistic effect between neighbouring Ag and Cu nanoparticles. We attribute the stabilization of the nanoparticles to the positive enthalpies of Cu-Ag solid solutions, which prevents the dissolution-redeposition induced particle growth under CO2RR conditions. The observed nanoparticle stabilization effect enables the design and fabrication of active CO2 reduction nanocatalysts with high durability.
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http://dx.doi.org/10.1039/d0nr09040aDOI Listing
March 2021

Risk Factors for Severe Residual Headache in Cerebral Venous Thrombosis.

Stroke 2021 Jan 7;52(2):531-536. Epub 2021 Jan 7.

China-America Institute of Neuroscience (K.J., C.Z., L.W., W.L., M.J., M.C., D.W., X.J.), Xuanwu Hospital, Capital Medical University, Beijing, China.

Background And Purpose: Which factors will influence the presence of severe residual headache after cerebral venous thrombosis (CVT) is unclear. The purpose of this study was to identify risk factors for severe residual headache in a large single-center cohort of patients with CVT.

Methods: We consecutively included eligible patients with CVT from a prospective stroke registry. Severe residual headache was defined as a residual headache attack requiring bed rest or hospital admission within 1 month before the last follow-up visit. We identified the risk factors of severe residual headache in all survivors and in those with favorable functional outcome (a modified Rankin Scale score, 0-2).

Results: A total of 325 patients' data were analyzed. At the last follow-up (median 13 months), 43 patients (13.2%) reported severe headache. In the multivariable analysis, isolated intracranial hypertension (odds ratio [OR], 3.309 [95% CI, 1.434-7.634]; =0.005), CVT recurrence (OR, 4.722 [95% CI, 1.639-13.602]; =0.004), and no recanalization (OR, 10.158 [95% CI, 4.194-24.600]; <0.001) were independently associated with severe headache. Severe headache was more frequent in patients with unfavorable outcome (11/25 [44.0%] versus 32/300 [10.7%]; <0.001). In patients with favorable outcome, the risk factors for severe headache were isolated intracranial hypertension (OR, 3.236 [95% CI, 1.268-8.256]; =0.014) and no recanalization (OR, 7.863 [95% CI, 3.120-19.812]; <0.001).

Conclusions: Isolated intracranial hypertension, CVT recurrence, and no recanalization increased the risk for severe residual headache after CVT.
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http://dx.doi.org/10.1161/STROKEAHA.120.029820DOI Listing
January 2021

SERPINC1 novel mutation (c.637C > T p. Gln213Ter) in a cerebral venous sinus thrombosis case and treatment with agatroban.

Thromb Res 2021 03 17;199:35-37. Epub 2020 Dec 17.

Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.

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http://dx.doi.org/10.1016/j.thromres.2020.12.011DOI Listing
March 2021

Association between high-sensitivity C-reactive protein levels and clinical outcomes in acute ischemic stroke patients treated with endovascular therapy.

Ann Transl Med 2020 Nov;8(21):1379

Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

Background: Increasing evidence demonstrates that high-sensitivity C-reactive protein (hs-CRP) is an independent prognostic predictor in acute ischemic stroke (AIS) patients. The purpose of this study is to investigate the association between hs-CRP levels and clinical outcomes in AIS patients receiving endovascular therapy (EVT).

Methods: This observational study was based on a prospective registry study. AIS patients receiving EVT from December 2012 to January 2019 were included. The modified Rankin Scale (mRS) scores at the 90-day and long-term follow-up were evaluated as clinical outcomes. Multivariable logistic regression analysis was conducted to adjust for confounders. Receiver operating characteristic (ROC) curve analysis was performed based on significant predictors of favorable outcomes in the logistic regression analysis. Patients were divided into two groups according to the cutoff value. Clinical outcomes were compared between groups. Survival probability was assessed using Kaplan-Meier survival analysis.

Results: Multivariable logistic regression analysis of the 362 enrolled AIS patients demonstrated that age (P=0.030), National Institutes of Health Stroke Scale (NIHSS) score (P=0.023), hs-CRP levels (P<0.001), and symptomatic intracranial hemorrhage (sICH) (P=0.006) were independently predictive of favorable outcomes. ROC curve analysis indicated that the hs-CRP level was predictive of favorable outcomes at the 90-day follow-up with a cutoff value of 8.255 mg/L. The mRS scores between patients with hs-CRP <8.255 mg/L and patients with hs-CRP ≥8.255 mg/L at the 90-day [2 (IQR, 1-2) 4 (IQR, 3-6), P<0.001] and long-term follow-up [1 (IQR, 0-2) 4 (IQR, 2-6), P<0.001] were significantly different. Patients with hs-CRP ≥8.255 mg/L had significantly increased risk of poor clinical outcomes at the 90-day and long-term follow-up compared with those with hs-CRP <8.255 mg/L (P<0.001 each).

Conclusions: Elevated hs-CRP levels were associated with poor clinical outcomes in AIS patients receiving EVT.
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http://dx.doi.org/10.21037/atm-20-3820DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723633PMC
November 2020

Intra-arterial Cold Saline Infusion in Stroke: Historical Evolution and Future Prospects.

Aging Dis 2020 Dec 1;11(6):1527-1536. Epub 2020 Dec 1.

3Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

Acute ischemic stroke (AIS) is a perpetual threat to life and functionality due to its high morbidity and mortality. In the past several decades, therapeutic hypothermia has garnered interest as an effective neuroprotective method in the setting of AIS. However, traditional hypothermic methods have been criticized for their low cooling efficiency and side effects. Intra-arterial cold saline infusion (IA-CSI), as a novel hypothermic method, not only minimizes these side effects, but is also perfectly integrated with widely accepted recanalization modalities in AIS, thereby serving as a promising prospect for clinical translation. In this article, we review the historical development of IA-CSI, summarize major studies of IA-CSI in rodents, large animals, and humans to date, and suggest insight into future development prospects in the field of AIS. We hope that this article will provide inspiration for the future application of hypothermia in AIS patients.
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http://dx.doi.org/10.14336/AD.2020.0325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673854PMC
December 2020

Author Correction: Continuous scanning for Bragg coherent X-ray imaging.

Sci Rep 2020 Nov 5;10(1):19420. Epub 2020 Nov 5.

CEA Grenoble, IRIG, MEM, NRS, Univ. Grenoble Alpes, 17 rue des Martyrs, 38000, Grenoble, France.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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http://dx.doi.org/10.1038/s41598-020-75649-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7645716PMC
November 2020

Long-term outcome of endovascular therapy for acute basilar artery occlusion.

J Cereb Blood Flow Metab 2021 06 21;41(6):1210-1218. Epub 2020 Sep 21.

Department of Neurosurgery, Capital Medical University, Beijing, China.

The long-term functional outcome of acute basilar artery occlusion (BAO) patients who received modern endovascular therapy (EVT) is unclear. We sought to assess the long-term functional outcome of BAO patients treated with EVT and determine the prognostic factors associated with favorable outcome. We enrolled consecutive BAO patients who received EVT between December 2012 and December 2018 in this observational study. Baseline characteristics and outcomes were presented. Multivariable logistic regression analysis was performed to identify the prognostic factors associated with long-term outcome. Among the 177 BAO patients included in this study, 80 patients (45.2%) obtained favorable outcome and 97 patients (54.8%) had unfavorable outcome at long-term follow-up with a median observation time of 12 months (interquartile range, 3-19). A total of 67 patients (37.9%) died. National Institutes of Health Stroke Scale (NIHSS), posterior circulation Alberta Stroke Program Early Computed Tomography Score (pc-ASPECTS), time from stroke onset to recanalization, and recanalization condition were identified as independent predictors for long-term outcome. Over 40% of BAO patients who were treated with modern EVT achieved favorable outcome at long-term follow-up. NIHSS, pc-ASPECTS, time from stroke onset to recanalization, and recanalization condition were identified as independent prognostic factors of long-term outcome.
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http://dx.doi.org/10.1177/0271678X20958587DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142131PMC
June 2021

Glycosylated Hemoglobin A1c Predicts Intracerebral Hemorrhage with Acute Ischemic Stroke Post-Mechanical Thrombectomy.

J Stroke Cerebrovasc Dis 2020 Sep 15;29(9):105008. Epub 2020 Jun 15.

Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China. Electronic address:

Background: Intracerebral hemorrhage, including symptomatic intracerebral hemorrhage, is a serious post-mechanical thrombectomy complication in patients with acute ischemic stroke. We aimed to determine whether glycosylated hemoglobin A1c parameters could predict intracerebral hemorrhage in this patient population.

Methods: We enrolled patients with acute occlusion of the internal carotid artery or proximal middle cerebral artery and who had undergone mechanical thrombectomy. According to the glycosylated hemoglobin A1c level (%) assessed during the hospital stay, the patients were divided into two groups: > 6.5% and ≤ 6.5%. Intracerebral hemorrhage was evaluated and classified based on cranial computed tomography scans obtained within 24-48 h or when neurological conditions worsened. We assessed the outcome at the end of 90 days using the modified Rankin Scale scores.

Results: Among 202 patients, 86 (42.6%) suffered intracerebral hemorrhage, while 25 (12.4%) had symptomatic intracerebral hemorrhage; 35.6% of the patients had a favorable outcome (modified Rankin Scale scores 0-2). Multivariable analysis demonstrated an association of glycosylated hemoglobin A1c > 6.5% with intracerebral hemorrhage. Furthermore, glycosylated hemoglobin A1c > 6.5% was independently associated with symptomatic intracerebral hemorrhage (OR, 2.136; 95% CI, 1.279-3.567; P = 0.004). In addition, glycosylated hemoglobin A1c > 6.5% was significantly associated with increased mortality (OR, 1.511; 95% CI, 1.042-2.191; P = 0.029) and negatively associated with favorable outcome (OR, 0.480; 95% CI, 0.296-0.781; P = 0.003) at 90 days.

Conclusions: Glycosylated hemoglobin A1c is an independent predictor of intracerebral hemorrhage (specifically, symptomatic intracerebral hemorrhage) in patients with acute ischemic stroke treated with mechanical thrombectomy. Further studies are needed to validate these findings.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2020.105008DOI Listing
September 2020

Continuous scanning for Bragg coherent X-ray imaging.

Sci Rep 2020 07 29;10(1):12760. Epub 2020 Jul 29.

CEA Grenoble, IRIG, MEM, NRS, Univ. Grenoble Alpes, 17 rue des Martyrs, 38000, Grenoble, France.

We explore the use of continuous scanning during data acquisition for Bragg coherent diffraction imaging, i.e., where the sample is in continuous motion. The fidelity of continuous scanning Bragg coherent diffraction imaging is demonstrated on a single Pt nanoparticle in a flow reactor at [Formula: see text] in an Ar-based gas flowed at 50 ml/min. We show a reduction of 30% in total scan time compared to conventional step-by-step scanning. The reconstructed Bragg electron density, phase, displacement and strain fields are in excellent agreement with the results obtained from conventional step-by-step scanning. Continuous scanning will allow to minimise sample instability under the beam and will become increasingly important at diffraction-limited storage ring light sources.
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http://dx.doi.org/10.1038/s41598-020-69678-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391662PMC
July 2020

Local anesthesia vs general anesthesia during endovascular therapy for acute posterior circulation stroke.

J Neurol Sci 2020 09 16;416:117045. Epub 2020 Jul 16.

Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China. Electronic address:

Objective: The optimal anesthetic approach during endovascular therapy (EVT) in acute stroke patients remains an area of uncertainty. We investigated the impact of different anesthetic approaches on the outcome of posterior circulation stroke (PCS) patients undergoing EVT.

Methods: For this observational study, we enrolled consecutive PCS patients who underwent EVT from December 2012 to December 2018, and compared functional outcomes at 90 days as well as long-term follow-up in patients treated under local anesthesia (LA) versus general anesthesia (GA). Multivariable logistic regression and propensity score matched analyses were conducted.

Results: Among the 183 patients included in this study, 71 patients (38.8%) received LA and 112 patients (61.2%) received GA. Median modified Rankin Scale score at 90 days was 4 (IQR, 2-6) in both groups (P = .956). No significant differences in the rates of functional independence and mortality at 90 days as well as long-term follow-up post intervention were observed between the two groups, and Kaplan-Meier survival analysis showed comparable long-term survival probabilities. Safety outcomes (including procedure-related complications and serious adverse events) did not differ between these patients. The anesthetic approach was neither associated with functional independence nor associated with mortality. Propensity score matched analysis indicated similar results.

Conclusions: For PCS patients undergoing EVT, LA compared with GA does not seem to result in different functional outcomes and complications rates.
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http://dx.doi.org/10.1016/j.jns.2020.117045DOI Listing
September 2020

Reperfusion plus Selective Intra-arterial Cooling (SI-AC) Improve Recovery in a Nonhuman Primate Model of Stroke.

Neurotherapeutics 2020 10;17(4):1931-1939

Department of neurology and China-America Institute of Neuroscience, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.

Early reperfusion is increasingly prioritized in ischemic stroke care, but outcomes remain suboptimal. Therefore, there is an urgent need to find neuroprotective approaches that can be combined with reperfusion to maximize efficacy. Here, the neuroprotective mechanisms behind therapeutic hypothermia were evaluated in a monkey model of ischemic stroke. Focal ischemia was induced in adult rhesus monkeys by placing autologous clots in the middle cerebral artery. Monkeys were treated with tissue plasminogen activator (t-PA) alone or t-PA plus selective intra-arterial cooling (SI-AC). Serial MRI scans and functional deficit were evaluated after ischemia. Histopathology and immunohistochemistry analysis were performed after the final MRI scan. t-PA plus SI-AC treatment led to a higher rate of MRI tissue rescue, and significantly improved neurologic deficits and daily activity scores compared with t-PA alone. In peri-infarct areas, higher fractional anisotropy values and greater fiber numbers were observed in models receiving t-PA plus SI-AC. Histological findings indicated that myelin damage, spheroids, and spongiosis were significantly ameliorated in models receiving SI-AC treatment. White matter integrity was also improved by SI-AC based on immunochemical staining. Our study demonstrates that SI-AC can be effectively combined with t-PA to improve both structural and functional recovery in a monkey model of focal ischemia. These findings provide proof-of-concept that it may be feasible to add neuroprotective agents as adjunctive treatments to reperfusion therapy for stroke.
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http://dx.doi.org/10.1007/s13311-020-00895-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851312PMC
October 2020

Intranasal salvinorin A improves neurological outcome in rhesus monkey ischemic stroke model using autologous blood clot.

J Cereb Blood Flow Metab 2021 04 2;41(4):723-730. Epub 2020 Jul 2.

Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

Salvinorin A (SA) exerts neuroprotection and improves neurological outcomes in ischemic stroke models in rodents. In this study, we investigated whether intranasal SA administration could improve neurological outcomes in a monkey ischemic stroke model. The stroke model was induced in adult male rhesus monkeys by occluding the middle cerebral artery M2 segment with an autologous blood clot. Eight adult rhesus monkeys were randomly administered SA or 10% dimethyl sulfoxide as control 20 min after ischemia. Magnetic resonance imaging was used to confirm the ischemia and extent of injury. Neurological function was evaluated using the Non-Human Primate Stroke Scale (NHPSS) over a 28-day observation period. SA significantly reduced infarct volume (3.9 ± 0.7 cm vs. 7.2 ± 1.0 cm; =0.002), occupying effect (0.3 ± 0.2% vs. 1.4 ± 0.3%; =0.002), and diffusion limitation in the lesion (-28.2 ± 11.0% vs. -51.5 ± 7.1%; =0.012) when compared to the control group. SA significantly reduced the NHPSS scores to almost normal in a 28-day observation period as compared to the control group (=0.005). Intranasal SA reduces infarct volume and improves neurological outcomes in a rhesus monkey ischemic stroke model using autologous blood clot.
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http://dx.doi.org/10.1177/0271678X20938137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983500PMC
April 2021

Ligand-free ZnS nanoparticles: as easy and green as it gets.

Chem Commun (Camb) 2020 Jul;56(61):8707-8710

Dipartimento di Scienze Chimiche, Università degli Studi di Padova and INSTM UdR, via Marzolo, 1, 35131, Padova, Italy.

The controlled nucleation and crystallization of small pure sphalerite ZnS nanoparticles was achieved under batch and continuous flow conditions at low temperature, in water and without the use of any stabilizing ligand. The obtained nanoparticles displayed a narrow size distribution and high specific surface area. Moreover, the synthesis was suitable to directly obtain stable water-based suspensions and the products were found to be active photocatalysts for the hydrogen evolution reaction.
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http://dx.doi.org/10.1039/d0cc01901aDOI Listing
July 2020

Anticoagulation delay does not affect the functional outcome of cerebral venous thrombosis.

Aging (Albany NY) 2020 06 18;12(12):11835-11842. Epub 2020 Jun 18.

China-America Institute of Neuroscience, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.

Available knowledge about the impact of anticoagulation delay on outcomes of patients with cerebral venous thrombosis (CVT) is limited. We therefore assessed the factors influencing anticoagulation delay and investigated the effect of this delay on outcomes of CVT patients. Anticoagulation delay was defined as the time interval between symptom onset and anticoagulation initiation. The primary outcome was a modified Rankin Scale (mRS) score > 2 at the final follow-up. A total of 164 eligible patients were included. The median anticoagulation delay was 9 days. Cerebral hemorrhage on admission neuroimaging correlated with earlier anticoagulation (p = 0.040). Anticoagulation delay was not associated with poor functional outcome (mRS > 2), but it was associated with residual headache across the entire cohort (earlier anticoagulation: 15/76 [19.7%] vs. later anticoagulation: 28/79 [35.4%]; p = 0.029) and in the subgroup with isolated intracranial hypertension (earlier anticoagulation: 4/25 [16.0%] vs. later anticoagulation: 14/27 [51.9%]; p = 0.007). Anticoagulation delay was found to be common among patients with CVT. Anticoagulation delay was not associated with poor functional outcome, but may have led to an increased risk of residual headache across our entire cohort and in the subgroup with isolated intracranial hypertension.
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http://dx.doi.org/10.18632/aging.103353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343482PMC
June 2020

The Influence of the Ligand in the Iridium Mediated Electrocatalyic Water Oxidation.

ACS Catal 2020 Apr 17;10(7):4398-4410. Epub 2020 Mar 17.

Leiden Institute of Chemistry, Leiden University, 2300 RA Leiden, The Netherlands.

Electrochemical water oxidation is the bottleneck of electrolyzers as even the best catalysts, iridium and ruthenium oxides, have to operate at significant overpotentials. Previously, the position of a hydroxyl on a series of hydroxylpicolinate ligands was found to significantly influence the activity of molecular iridium catalysts in sacrificial oxidant driven water oxidation. In this study, these catalysts were tested under electrochemical conditions and benchmarked to several other known molecular iridium catalysts under the exact same conditions. This allowed us to compare these catalysts directly and observe whether structure-activity relationships would prevail under electrochemical conditions. Using both electrochemical quartz crystal microbalance experiments and X-ray photoelectron spectroscopy, we found that all studied iridium complexes form an iridium deposit on the electrode with binding energies ranging from 62.4 to 62.7 eV for the major Ir 4f species. These do not match the binding energies found for the parent complexes, which have a broader binding energy range from 61.7 to 62.7 eV and show a clear relationship to the electronegativity induced by the ligands. Moreover, all catalysts performed the electrochemical water oxidation in the same order of magnitude as the maximum currents ranged from 0.2 to 0.6 mA cm once more without clear structure-activity relationships. In addition, by employing H NMR spectroscopy we found evidence for Cp* breakdown products such as acetate. Electrodeposited iridium oxide from ligand free [Ir(OH)] or a colloidal iridium oxide nanoparticles solution produces currents almost 2 orders of magnitude higher with a maximum current of 11 mA cm. Also, this deposited material contains, apart from an Ir 4f species at 62.4 eV, an Ir species at 63.6 eV, which is not observed for any deposit formed by the molecular complexes. Thus, the electrodeposited material of the complexes cannot be directly linked to bulk iridium oxide. Small IrO clusters containing few Ir atoms with partially incorporated ligand residues are the most likely option for the catalytically active electrodeposit. Our results emphasize that structure-activity relationships obtained with sacrificial oxidants do not necessarily translate to electrochemical conditions. Furthermore, other factors, such as electrodeposition and catalyst degradation, play a major role in the electrochemically driven water oxidation and should thus be considered when optimizing molecular catalysts.
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http://dx.doi.org/10.1021/acscatal.0c00531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137537PMC
April 2020

Primate Version of Modified Rankin Scale for Classifying Dysfunction in Rhesus Monkeys.

Stroke 2020 05 1;51(5):1620-1623. Epub 2020 Apr 1.

From the Department of Neurology and China-America Institute of Neuroscience (D.W., L.W., J.C., X.H., Y.D., X.J.), Xuanwu Hospital, Capital Medical University, Beijing.

Background and Purpose- Nonhuman primates are increasingly used in translational studies of ischemic stroke. However, current scoring systems in monkeys (eg, Nonhuman Primate Stroke Scale) do not focus on impairments in activities of daily living, so clinically relevant data are scarce for evaluating functional deficits in this model. Methods- Here, we referenced the modified Rankin Scale to provide a primate version of Rankin Scale (pRS) for ranking neurological dysfunction in monkeys following stroke. We selected hand function and strength, level of activity, and general mobility as the main components of pRS. We also analyzed interobserver variability. Results- pRS is a simple scale with only 6 levels. Functional deficit can be easily classified into none (category 0), slight (categories 1-2), moderate (category 3-4), and severe disabilities (category 5) based on pRS. We validated this scoring system on 11 monkeys, all with varying levels of neurological dysfunction following stroke, assessed by blinded testers. After a short training period, both technicians and neurology residents were able to achieve a high level of consistency using this scoring system. Conclusions- pRS is a simple and reliable functional scale, similar to the widely used modified Rankin Scale, for evaluating long-term neurological dysfunction in nonhuman primates. We recommend further validation studies and analyses.
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http://dx.doi.org/10.1161/STROKEAHA.119.028108DOI Listing
May 2020

Prognosis and risk factors for reocclusion after mechanical thrombectomy.

Ann Clin Transl Neurol 2020 04 10;7(4):420-428. Epub 2020 Mar 10.

Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico Health Sciences Center, Albuquerque, New Mexico.

Objective: This study evaluates reocclusion prognostic outcomes and explores reocclusion risk factors after mechanical thrombectomy (MT) in Chinese stroke patients.

Methods: Altogether, 614 patients with AIS with successful recanalization after MT were recruited in this study and divided into the reocclusion and the non-reocclusion group depending on the 24-h imaging results after MT. Differences between the two groups were compared including 24-h and 7-day National Institutes of Health Stroke Scale (NIHSS) scores, 90-day modified Rankin scale(mRS) scores, good prognosis (mRS:0-2) rates, incidence of intracranial hemorrhage, and 90-day mortality.

Results: Forty-four (7.2%) patients experienced reocclusion within 24 h. Compared with the non-reocclusion group, patients in the reocclusion group had higher 24-h (15 vs. 13) and 7-day (15 vs. 9) NIHSS scores, 90-day mRS scores (4 vs. 3), and 90-day mortality rates (34.1% vs. 18.6%); lower rates of good prognosis (13.6% vs. 9.3%); and a higher incidence of early neurological deterioration (36.4% vs. 14.7%). Age, internal carotid artery occlusion (ICA), intravenous thrombolysis (IVT), number of thrombectomy passes, stent implantation, and levels of D-dimer (adjusted odds ratio and 95% confidence interval: 0.97, 0.94-0.99; 2.40, 1.10-5.23; 2.21, 1.05-4.66; 2.60, 1.04-6.47; 0.25, 0.09-0.67; and 1.06, 1.01-1.12, respectively) were independently associated with 24-h reocclusion.

Interpretation: The prognosis of reocclusion after MT was poor. Timely evaluation of these factors including age, D-dimer, ICA occlusion, IVT, number of passes, and stent implantation and appropriate intervention could reduce the incidence of reocclusion for Chinese stroke patients.
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http://dx.doi.org/10.1002/acn3.50999DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187702PMC
April 2020

Letter by Wu et al Regarding Article, "Thrombectomy and Thrombolysis of Isolated Posterior Cerebral Artery Occlusion: Cognitive, Visual, and Disability Outcomes".

Stroke 2020 04 4;51(4):e68. Epub 2020 Mar 4.

Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

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http://dx.doi.org/10.1161/STROKEAHA.119.028706DOI Listing
April 2020

Selective intra-arterial brain cooling improves long-term outcomes in a non-human primate model of embolic stroke: Efficacy depending on reperfusion status.

J Cereb Blood Flow Metab 2020 07 3;40(7):1415-1426. Epub 2020 Mar 3.

Department of Neurology and China-America Institute of Neuroscience, Xuanwu Hospital, Capital Medical University, Beijing, China.

Nearly all stroke neuroprotection modalities, including selective intra-arterial cooling (SI-AC), have failed to be translated from bench to bed side. Potentially overlooked reasons may be biological gaps, inadequate attention to reperfusion states and mismatched attention to neurological benefits. To advance stroke translation, we describe a novel thrombus-based stroke model in adult rhesus macaques. Intra-arterial thrombolysis with tissue plasminogen activator leads to three clinically relevant outcomes - complete, partial, and no recanalization based on digital subtraction angiography. We also find reperfusion as a prerequisite for SI-AC-induced benefits, in which models with complete or partial reperfusion exhibit significantly reduced infarct volumes, mitigated neurological deficits, improved upper limb motor dysfunction in both acute and chronic stages; however, no further neuroprotection is observed in those without reperfusion. In summary, we discover reperfusion as a crucial regulator of SI-AC-induced neuroprotection and provide insights of long-term functional benefits in behavior and imaging levels. Our findings could be important not only for the translational prerequisite and potential molecular targets, but also for this thrombus-thrombolysis model in monkeys as a powerful tool for further translational stroke studies.
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http://dx.doi.org/10.1177/0271678X20903697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7308521PMC
July 2020

Large area, patterned growth of 2D MoS and lateral MoS-WS heterostructures for nano- and opto-electronic applications.

Nanotechnology 2020 Apr 12;31(25):255603. Epub 2020 Feb 12.

Department of Applied Physics, Eindhoven University of Technology, PO Box 513, 5600 MB Eindhoven, The Netherlands.

The patterned growth of transition metal dichalcogenides (TMDs) and their lateral heterostructures is paramount for the fabrication of application-oriented electronics and optoelectronics devices. However, the large scale patterned growth of TMDs remains challenging. Here, we demonstrate the synthesis of patterned polycrystalline 2D MoS thin films on device ready SiO/Si substrates, eliminating any etching and transfer steps using a combination of plasma enhanced atomic layer deposition (PEALD) and thermal sulfurization. As an inherent advantage of ALD, precise thickness control ranging from a monolayer to few-layered MoS has been achieved. Furthermore, uniform films with exceptional conformality over 3D structures are obtained. Finally, the approach has been leveraged to obtain in-plane lateral heterostructures of 2D MoS and WS thin films over a large area which opens up an avenue for their direct integration in future nano- and opto-electronic device applications.
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http://dx.doi.org/10.1088/1361-6528/ab7593DOI Listing
April 2020

Acute total occlusion of left circumflex artery in a patient with dextrocardia and situs inversus.

J Int Med Res 2020 Jan;48(1):300060519893180

Department of Cardiology, Bazhong Municipal Hospital of Traditional Chinese Medicine, Bazhong, China.

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http://dx.doi.org/10.1177/0300060519893180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254168PMC
January 2020

Relationship between elevated plasma trimethylamine N-oxide levels and increased stroke injury.

Neurology 2020 02 6;94(7):e667-e677. Epub 2020 Jan 6.

From the Departments of Neurology (C.W., J.Z., D.W., F.C., W.W., W.Z., L. Wu, H.S., Q.M.), Evidence-Based Medicine (K.Y.), and Neurosurgery (X.J.), Xuanwu Hospital Capital Medical University, Beijing; Department of Neurology (F.X.), Second Hospital of Hebei Medical University, Shijiazhuang; Department of Neurology (Y.L., N.X.), First Affiliated Hospital of Zhengzhou University, Henan; Department of Neurology (W.C.), Second People's Hospital of Liaocheng, Shandong; and Department of Neurology (L. Wang), Fifth Affiliated Hospital of Zhengzhou University, Henan, China.

Objective: To investigate whether elevated plasma trimethylamine N-oxide (TMAO) levels are associated with initial stroke severity and infarct volume.

Methods: This cross-sectional study included 377 patients with acute ischemic stroke and 50 healthy controls. Plasma TMAO levels were assessed at admission. Stroke infarct size and clinical stroke severity were measured with diffusion-weighted imaging and the NIH Stroke Scale (NIHSS). Mild stroke was defined as an NIHSS score <6.

Results: Plasma TMAO levels were higher in patients with ischemic stroke than in healthy controls (median 5.1 vs 3.0 μmol/L; < 0.001). Every 1-µmol/L increase in TMAO was associated with a 1.13-point increase in NIHSS score (95% confidence interval [CI] 1.04-1.29; < 0.001) and 1.69-mL increase in infarct volume (95% CI 1.41-2.03; < 0.001) after adjustment for vascular risk factors. At admission, 159 patients (42.2%) had experienced a mild stroke, and their plasma TMAO levels were lower compared to those with moderate to severe stroke (median 3.6 vs 6.5 µmol/L; < 0.001). The area under the receiver operating characteristics curve of plasma TMAO level in predicting moderate to severe stroke was 0.794 (95% CI 0.748-0.839; < 0.001), and the optimal cutoff value was 4.95 μmol/L. The sensitivity and specificity of TMAO levels ≥4.95 μmol/L for moderate to severe stroke were 70.2% and 79.9%, respectively.

Conclusions: Patients with ischemic stroke had higher plasma TMAO levels compared to healthy controls. Higher plasma TMAO level at admission is an independent predictor of stroke severity and infarct volume in patients with acute ischemia.
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http://dx.doi.org/10.1212/WNL.0000000000008862DOI Listing
February 2020

Hypothermic neuroprotection against acute ischemic stroke: The 2019 update.

J Cereb Blood Flow Metab 2020 03 19;40(3):461-481. Epub 2019 Dec 19.

Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

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http://dx.doi.org/10.1177/0271678X19894869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7026854PMC
March 2020

Identification of PBMC-expressed miRNAs for rheumatoid arthritis.

Epigenetics 2020 04 10;15(4):386-397. Epub 2019 Oct 10.

Center for Genetic Epidemiology and Genomics, School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu, P. R. China.

Post-transcriptional regulation by miRNAs plays an important role in the pathogenesis of rheumatoid arthritis (RA), however, the roles of specific miRNAs in RA pathogenesis remain largely unclear. This study performed dual-omics (miRNA and mRNA) integration analysis and in-depth cellular and molecular functional exploration to identify novel RA-associated miRNAs and to understand their underlying pathogenic mechanism. Based on the miRNA and mRNA expression profiles in peripheral blood mononuclear cells (PBMCs) from a discovery sample set (25 RA cases and 18 healthy controls), 18 differentially expressed miRNAs (DEMIRs) (|Fold-change|>2 and P < 0.05) were identified and corresponding interaction networks of DEMIRs and mRNA were constructed. After the expression validation of the DEMIRs in a validation sample set (35 RA cases and 35 healthy controls), miR-99b-5p was highlighted. The over-expression of newly discovered miR-99b-5p is able to suppress T cell apoptosis, promote cell proliferation and activation, increase expression of proinflammatory cytokines (IL-2, IL-6, TNF-α, and IFN-γ), and inhibit expression of its target genes mTOR and RASSF4. This study comprehensively identified PBMC-expressed miRNAs along with corresponding regulatory networks significant for RA and discovered miR-99b-5p as a novel post-transcriptional mediator involved in RA pathogenesis. The findings improved our understanding of RA pathogenesis and provided novel insights into the molecular mechanisms underlying RA pathogenesis.
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http://dx.doi.org/10.1080/15592294.2019.1676613DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153549PMC
April 2020
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