Publications by authors named "Long Zhao"

534 Publications

Effect of transcranial direct current stimulation combined with a smart hand joint training device on hand dysfunction in patients with early stroke.

Folia Neuropathol 2022 ;60(2):177-184

The Department of Rehabilitation, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Introduction: The aim of this study was to investigate the effect of transcranial direct current stimulation (tDCS) combined with a smart hand joint training device on hand dysfunction in patients with early stroke.

Material And Methods: This study was a randomized controlled trial, which was conducted in the neurology department in our hospital. From March 2019 to January 2021, 60 patients diagnosed with early stroke hand dysfunction were selected. A random number table method was used to divide patients equally into control group (smart hand joint training device group) and intervention group (tDCS and smart hand joint training device group). Before and after treatment, Brunnstrom six-level staging, Fugl-Meyer motor function score (wrist hand part), hemiplegic finger function examination, and hemiplegic hand function classification evaluation were applied in both groups. Main outcome measures were Brunnstrom motor function staging (hand part), functional evaluation of hemiplegic fingers, and Fugl-Meyer motor function score (wrist hand part).

Results: After treatment, compared with control group, the results in intervention group of Brunnstrom six-level staging and hemiplegic hand function classification evaluation showed obvious improvement (p = 0.000), and the result of hemiplegic fingers' functional evaluation also improved (p = 0.026). After treatment, Fugl-Meyer motor function scores were 6.73 ±6.65 (control group) and 9.8 ±6.66 (intervention group). Slight tDCS-related adverse events occurred in one patient (3.33%) in intervention group. None in either group discontinued treatment.

Conclusions: Both the smart hand joint training device alone and tDCS combined with the smart hand joint training device can improve hand function of patients with early stroke to varying degrees, but the treatment effect of tDCS combined with the smart hand joint training device is more significant.
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http://dx.doi.org/10.5114/fn.2022.117534DOI Listing
August 2022

Promotion of Catalytic Oxygen Reduction Reactions: The Utility of Proton Management Substituents on Cobalt Porphyrins.

Inorg Chem 2022 Aug 9. Epub 2022 Aug 9.

School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, P. R. China.

Three ABAB-type cobalt -tetraarylporphyrins with fluorine (), acetic acid (), and cyanoacetic acid () groups at the -positions of phenyl rings at the 10,20-positions are synthesized and evaluated as catalysts for oxygen reduction reactions (ORRs). In density functional theory calculations, the frontier molecular orbitals of these complexes were found to be stabilized relative to model complexes with electron-withdrawing atoms or moieties on the -aryl rings. Electrochemical measurements suggest that electrodes with ( exhibit the most positive ORR potential values and the highest limiting current density in both acidic and alkali electrolytes, while the electrocatalyst exhibits extremely low ORR performance. The electron transfer numbers for the electrocatalysts are more than 3.0, indicating that a mixture of 2- and 4-electron transfer pathways occurs. The results demonstrate that coupling the hydrogen bonding properties and electron-withdrawing abilities through rational design of the substituent at the -position is an efficient way to modify the ORR performance.
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http://dx.doi.org/10.1021/acs.inorgchem.2c01591DOI Listing
August 2022

Clinical effect of pulmonary rehabilitation combined with diaphragm pacemaker therapy in the treatment of severely ill patients with mechanical ventilation.

Int J Rehabil Res 2022 Sep 23;45(3):195-200. Epub 2022 Jun 23.

Department of Rehabilitation, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

To investigate the effect of pulmonary rehabilitation combined with diaphragm pacemaker therapy on the diaphragm function of severely ill patients on mechanical ventilation. Forty patients were randomly divided into the control group (CG; n = 20) and experimental group (EG; n = 20). The CG was given basic ICU nursing and conventional rehabilitation treatment. The EG added a diaphragm pacemaker and pulmonary rehabilitation therapies along with basic ICU nursing and conventional rehabilitation treatment. The relevant indexes were compared at baseline and postintervention, including the Glasgow Coma Scale (GCS), Acute Physiology, Chronic Health Evaluation II (APACHE II) scores, diaphragm mobility and thickness. The indexes of mechanical ventilation time, ICU and total hospital stays were compared between the two groups. There was no difference in the GCS and APACHE II scores, and diaphragm mobility and thickness between the two groups before treatment (P > 0.05). After 30 days of treatment, the GCS scores increased in both groups, the APACHE II scores decreased significantly, and diaphragm mobility and thickness decreased compared with before treatment (P < 0.05), but the improvement of each index in the EG was more evident than in the CG. Compared with the CG, the indexes of the EG, including offline, ICU and total hospitalization times, were significantly shorter (P < 0.05). Additionally, there were no adverse events such as accidental tube detachment or falling out of bed during treatment. Pulmonary rehabilitation combined with a diaphragm pacemaker is safe and effective in treating severely ill mechanically ventilated patients.
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http://dx.doi.org/10.1097/MRR.0000000000000535DOI Listing
September 2022

Neuronavigated repetitive transcranial stimulation improves neurocognitive functioning in veterans with schizophrenia: a possible role of BDNF polymorphism.

Curr Neuropharmacol 2022 Aug 3. Epub 2022 Aug 3.

Peking University HuiLongGuan Clinical Medical School, Beijing HuiLongGuan Hospital, Beijing, China.

Previous literatures have reported that high-frequency (HF) neuronavigated repetitive transcranial magnetic stimulation (rTMS) may improve neurocognitive functioning in patients with schizophrenia. Nonetheless, the heterogeneity of the research findings with regards to the effectiveness of HF-rTMS on the neurocognitive functioning in patients with schizophrenia greatly hinders its clinical application. The current study was designed to determine the predictive role of BDNF variants for neurocognitive improvements after rTMS administration in veterans with schizophrenia. 109 hospitalized veterans with schizophrenia were randomly allocated to active 10Hz rTMS (n=63) or sham stimulation (n=46) over left DLPFC for 4 consecutive weeks. Neurocognitive functions were assessed by using the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS) at baseline and at the end of week 4. BDNF polymorphism was genotyped by the technicians. Compared with sham stimulation sessions, the immediate memory performance was significantly increased in active sessions after neuronavigated rTMS administration. In addition, patients with the CC homozygotes demonstrated greater improvement of immediate memory after rTMS treatment, while T allele carriers showed no significant improvement in immediate memory domain relative to baseline performance of immediate memory. Our findings suggest that add-on neuronavigated 10Hz rTMS is beneficial on immediate memory only in patients with CC homozygotes, but not in T allele carriers. This pilot study provides further evidence for BDNF as a promise biomarker in predicting the clinical response to rTMS stimulation.
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http://dx.doi.org/10.2174/1570159X20666220803154820DOI Listing
August 2022

Clinical efficacy of hyperbaric oxygen combined with different timings of right median-nerve electrical stimulation in patients with brain injury-induced disorders of consciousness.

Brain Behav 2022 Aug 3:e2716. Epub 2022 Aug 3.

The Scond Department of Rehabilitation, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Objective: The objective of this study was to investigate the clinical efficacy of hyperbaric oxygen combined with right median-nerve stimulation (RMNES) in patients with disorders of consciousness caused by brain injury.

Methods: A total of 120 patients with consciousness disorders caused by brain injury were selected. They were randomly divided into three groups, a control group, test group 1 (treated with RMNES after hyperbaric oxygen therapy [HBOT]), and test group 2 (treated with RMNES at the same time as HBOT), with 40 patients in each group. Before and after treatment, the Glasgow coma scale (GCS), brainstem auditory-evoked potential (BAEP), electroencephalogram (EEG), and upper-limb sensory-evoked potential (USEP) were evaluated for the three groups of patients.

Results: The GCS score of patients in the three groups significantly improved compared with that before treatment (p < .05). There were significant differences in GCS scores among the three groups (p < .05), and the GCS score for the patients was test group 2>test group 1>control group. The EEG, BAEP, and USEP scores were significantly improved compared with those before treatment (p < .05), and the degree of improvement of patients in the three groups was test group 2>test group 1>control group (p < .05). The clinical efficacy of test group 2 was higher than that of test group 1, and the clinical efficacy of test group 1 was higher than that of the control group (p < .05).

Conclusion: Hyperbaric oxygen combined with RMNES can improve the state of consciousness and promote the recovery of consciousness for patients with consciousness disorders caused by brain injury, and the effect of RMNES combined with HBOT in the chamber on improving the recovery of consciousness is better than after HBOT outside the chamber.
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http://dx.doi.org/10.1002/brb3.2716DOI Listing
August 2022

Comparison of high flow nasal cannula and non-invasive positive pressure ventilation in children with bronchiolitis: A meta-analysis of randomized controlled trials.

Front Pediatr 2022 15;10:947667. Epub 2022 Jul 15.

Department of Respiratory, Central Hospital, Shenyang Medical College, Shenyang, China.

Background: The effects of high-flow nasal cannula (HFNC) compared to non-invasive positive pressure ventilation (NIPPV) on children with bronchiolitis remain unclear.

Methods: This meta-analysis was performed following the preferred reporting items for systematic reviews and meta-analysis (PRISMA) statement. Randomized controlled trials (RCTs) were identified from a comprehensive search in PubMed, EMBASE, Cochrane Library, and Web of Science without time and language limitations. Primary endpoints include the rate of treatment failure, the rate of need for intubation, and the pediatric intensive care unit (PICU) length of stay.

Results: Five RCTs including 541 children of less than 24 months were enrolled in the meta-analysis. Compared to the NIPPV group, the rate of treatment failure was significantly higher in the HFNC treatment group ( = 0.0%, = 0.574; RR 1.523, 95% CI 1.205 to 1.924, < 0.001). No significant difference was noted in the need for intubation ( = 0.0%, = 0.431; RR 0.874, 95% CI 0.598 to 1.276, = 0.485) and the PICU length of stay ( = 0.0%, = 0.568; WMD = -0.097, 95% CI = -0.480 to 0.285, = 0.618) between the HFNC group and the NIPPV treatment.

Conclusion: Compared to the NIPPV group, HFNC therapy was associated with a significantly higher treatment failure rate in children suffering from bronchiolitis. The intubation rate and the PICU length of stay were comparable between the two approaches.
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http://dx.doi.org/10.3389/fped.2022.947667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334708PMC
July 2022

Synergistic catalysis of graphitic carbon nitride supported bimetallic sulfide nanostructures for efficient oxygen generation.

Chem Commun (Camb) 2022 Jul 27. Epub 2022 Jul 27.

School of Chemistry & Chemical Engineering, Jiangsu University, Zhenjiang, 212013, P. R. China.

Herein, a series of g-CN supported bimetallic sulfide nanostructures (NiS/MoS/g-CN, = 10, 20 and 30) was prepared by a hydrothermal method and subsequently a thermal annealing approach. NiS/MoS/20g-CN with controlled composition exhibits efficient OER activity with a low overpotential of 183 mV at 10 mA cm, which outperforms the vast majority of sulfide OER electrocatalysts reported previously.
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http://dx.doi.org/10.1039/d2cc03619cDOI Listing
July 2022

An analgesic peptide H-20 attenuates chronic pain via the PD-1 pathway with few adverse effects.

Proc Natl Acad Sci U S A 2022 Aug 25;119(31):e2204114119. Epub 2022 Jul 25.

Center for Basic Medical Research, Medical School of Nantong University, Nantong 226001, Jiangsu Province, China.

The lack of effective and safe analgesics for chronic pain management has been a health problem associated with people's livelihoods for many years. Analgesic peptides have recently shown significant therapeutic potential, as they are devoid of opioid-related adverse effects. Programmed cell death protein 1 (PD-1) is widely expressed in neurons. Activation of PD-1 by PD-L1 modulates neuronal excitability and evokes significant analgesic effects, making it a promising target for pain treatment. However, the research and development of small molecule analgesic peptides targeting PD-1 have not been reported. Here, we screened the peptide H-20 using high-throughput screening. The in vitro data demonstrated that H-20 binds to PD-1 with micromolar affinity, evokes Src homology 2 domain-containing tyrosine phosphatase 1 (SHP-1) phosphorylation, and diminishes nociceptive signals in dorsal root ganglion (DRG) neurons. Preemptive treatment with H-20 effectively attenuates perceived pain in naïve WT mice. Spinal H-20 administration displayed effective and longer-lasting analgesia in multiple preclinical pain models with a reduction in or absence of tolerance, abuse liability, constipation, itch, and motor coordination impairment. In summary, our findings reveal that H-20 is a promising candidate drug that ameliorates chronic pain in the clinic.
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http://dx.doi.org/10.1073/pnas.2204114119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351488PMC
August 2022

Serous Cystadenoma of the Pancreas Showing Increased Uptake on 68Ga-FAPI PET/CT.

Clin Nucl Med 2022 Jul 19. Epub 2022 Jul 19.

From the Department of Nuclear Medicine and Minnan PET Center, Xiamen Cancer Center, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.

Abstract: Serous cystadenoma of the pancreas is a type of pancreatic cystic neoplasm frequently found in clinical practice. Serous cystadenoma of the pancreas can have a varied morphological presentation on imaging examinations. Therefore, it is sometimes very challenging to differentiate serous cystadenoma of the pancreas from pancreatic malignancy. In this study, we present a case of serous cystadenoma of the pancreas showing focally elevated radiotracer uptake on 68Ga-FAPI PET/CT. Considering potential future clinical use of 68Ga-FAPI PET/CT in malignancy, awareness of the false-positive findings is important for the differential diagnosis and management of these lesions.
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http://dx.doi.org/10.1097/RLU.0000000000004348DOI Listing
July 2022

Comparative genomic and transcriptomic analyses uncover the molecular basis of high nitrogen use efficiency in the wheat cultivar Kenong 9204.

Mol Plant 2022 Jul 20. Epub 2022 Jul 20.

Computer Network Information Center, Chinese Academy of Sciences, Beijing 100190, China.

Studying the regulatory mechanisms driving nitrogen use efficiency (NUE) of crops is important for sustaining agriculture and protecting environment. In this study, we generated a high-quality genome assembly for a high-NUE wheat cultivar Kenong 9204 and systematically analyzed the genes related to nitrogen uptake and metabolism. By comparative analysis, we found that the high-affinity nitrate transporter gene family expanded in Triticeae. Further study showed that subsequent functional differentiation endowed the expanded family members with salinity-inducible ability, which provides a genetic basis for improving the adaptability of wheat to nitrogen deficiency in various habitats. To explore the genetic and molecular mechanisms of high NUE, we compared the genomic and transcriptomic data between the high NUE cultivar KN9204 and a low-NUE cultivar Jing 411, and quantified their nitrogen accumulation under high and low nitrogen conditions. Compared with Jing 411, Kenong 9204 absorbed significantly more nitrogen at reproductive stage after shooting and accumulated in its shoots and seeds. Transcriptome data analysis revealed that nitrogen deficiency obviously suppressed the expression of the genes related to cell division in young spike of Jing 411, while such suppressed effect on gene expression was much less in Kenong 9204. In addition, Kenong 9204 maintained a longer time to keep a relative high expression of NPF genes than Jing 411 during seeds maturity. Physiological and transcriptome data revealed that Kenong 9204 was more tolerant to nitrogen deficiency than Jing 411, especially at its reproductive stage. The high NUE of KN9204 is an integrated effect controlled at different levels. Overall, our data obtained in this study provide a new insight to help understand the molecular mechanisms of NUE and a potential gene resource for improving wheat cultivars with higher NUE trait.
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http://dx.doi.org/10.1016/j.molp.2022.07.008DOI Listing
July 2022

Metformin Alleviates Delayed Hydrocephalus after Intraventricular Hemorrhage by Inhibiting Inflammation and Fibrosis.

Transl Stroke Res 2022 Jul 19. Epub 2022 Jul 19.

Department of Neurosurgery and State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, People's Republic of China.

Intraventricular hemorrhage (IVH) is a subtype of intracerebral hemorrhage (ICH) with high morbidity and mortality. Posthemorrhagic hydrocephalus (PHH) is a common and major complication that affects prognosis, but the mechanism is still unclear. Inflammation and fibrosis have been well established as the major causes of PHH after IVH. In this study, we aimed to investigate the effects of metformin on IVH in adult male mice and further explored the underlying molecular mechanisms of these effects. In the acute phase, metformin treatment exerted dose-dependent neuroprotective effects by reducing periependymal apoptosis and neuronal degeneration and decreasing brain edema. Moreover, high-dose metformin reduced inflammatory cell infiltration and the release of proinflammatory factors, thus protecting ependymal structure integrity and subependymal neurons. In the chronic phase, metformin administration improved neurocognitive function and reduced delayed hydrocephalus. Additionally, metformin significantly inhibited basal subarachnoid fibrosis and ependymal glial scarring. The ependymal structures partially restored. Mechanically, IVH reduced phospho-AMPK (p-AMPK) and SIRT1 expression and activated the phospho-NF-κB (p-NF-κB) inflammatory signaling pathway. However, metformin treatment increased AMPK/SIRT1 expression and lowered the protein expression of p-NF-κB and its downstream inflammation. Compound C and EX527 administration reversed the anti-inflammatory effect of metformin. In conclusion, metformin attenuated neuroinflammation and subsequent fibrosis after IVH by regulating AMPK /SIRT1/ NF-κB pathways, thereby reducing delayed hydrocephalus. Metformin may be a promising therapeutic agent to prevent delayed hydrocephalus following IVH.
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http://dx.doi.org/10.1007/s12975-022-01026-3DOI Listing
July 2022

Effect of transcranial direct current stimulation combined with respiratory training on dysphagia in post-stroke patients.

Technol Health Care 2022 Jul 8. Epub 2022 Jul 8.

The Second Department of Rehabilitation, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Background: Transcranial direct current stimulation (tDCS) has a considerable advantage in the rehabilitation treatment of dysphagia.

Objective: The purpose of this study was to explore the effect of tDCS combined with respiratory training on dysphagia in post-stroke patients.

Methods: From December 2017 to January 2019, 64 post-stroke patients who were hospitalized in the Department of Neurology of the Second Hospital of Hebei Medical University were enrolled in this study. They were randomly divided into control and treatment groups (n= 32). Patients in the two groups received routine swallowing rehabilitation training and respiratory training. On this basis, the patients in the treatment group received tDCS. The anode was placed in the movement area of the pharyngeal cortex on the unaffected side of the patients' bodies, and the cathode was placed in the upper orbital orbit on the opposite side. The current intensity was 1.5 mA, 20 min/time, 1 time/d, and 6 d/w. Before and after the treatment, the water swallow text, functional oral intake scale (FOIS), forced vital capacity (FVC) and peak expiratory flow (PEF) were assessed, and the correlation among them was evaluated.

Results: There were no differences in all indexes before and after treatment. After treatment, water swallow text, FOIS, FVC and PEF were all better than before treatment, and the clinical efficacy in the treatment group was significantly better than that in the control group. FVC and PEF were positively correlated with water swallow text and FOIS.

Conclusion: tDCS combined with respiratory training may have a significant therapeutic effect on dysphagia in post-stroke patients.
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http://dx.doi.org/10.3233/THC-213234DOI Listing
July 2022

Clinicopathological, Oncogenic, and F-FDG PET/CT Features of Primary Pulmonary Carcinoid in Resection Specimens.

Contrast Media Mol Imaging 2022 15;2022:1926797. Epub 2022 Jun 15.

Department of Nuclear Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai 200092, China.

Objectives: The metabolic parameters which included mean standardised uptake value (SUVmean), metabolic tumour volume (MTV), total lesion glycolysis (TLG), maximum standardised uptake lean body mass (SULmax), and maximum standardised uptake body surface area (SUVbsa) have rarely been investigated in pulmonary carcinoid (PC). This study aimed to retrospectively compare the F-FDG PET/CT features of PC subtypes and observe clinicopathological and oncogenic characteristics of PC.

Methods: We performed a retrospective review in 60 patients with PC, from January 2016 to November 2021, who underwent the F-FDG PET/CT scan. All the PC diagnoses were histopathologic confirmed by surgical samples. The metabolic and morphological features were obtained from F-FDG PET/CT images. The ratio of metabolic to morphological lesion volumes (MMVR) was calculated.

Results: Sixty patients with PC were consecutively identified, including 39 patients (65.0%) with typical carcinoids (TCs) and 21 (35.0%) with atypical carcinoids (ACs). One (1/21) patient had mutation in BRAF. The ACs have a larger size ( < 0.001), more metastatic lymph nodes ( = 0.011), higher Ki-67 expression ( < 0.001), higher SUVmax values ( = 0.003), higher SUVmean values ( = 0.006), higher SULmax values ( = 0.005), higher SUVbsa values ( = 0.001), higher MTV values ( = 0.033), and higher TLG values ( = 0.002). The multivariate analysis showed that MMVR ( = 0.020) was significantly associated with AC. For predicting AC, the optimal cut-off value of SUVmax, SUVmean, SULmax, SUVbsa, MTV, TLG, and the maximum diameter was 5.19, 3.18, 2.65, 1.47, 4.36, 18.44, and 3.0, respectively. The AUC values of above mentioned parameters was 0.756 (95%CI, 0.631-881;  = 0.001), 0.735 (95%CI, 0.602-868;  = 0.003), 0.736 (95%CI, 0.607-865;  = 0.003), 0.742 (95%CI, 0.612-873;  = 0.002), 0.593 (95%CI, 0.430-755;  = 0.239), 0.680 (95%CI, 0.531-829;  = 0.022), and 0.733 (95%CI, 0.598-868;  = 0.003), respectively. For predicting TC, the optimal cut-off value of the MMVR was 0.92, and the AUC value was 0.780 (95%CI, 0.647-0.913;  < 0.001).

Conclusion: F-FDG PET/CT can simultaneously reveal the metabolic and morphological characteristics of PC, which is important in the differentiation for histopathologic subtypes.
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http://dx.doi.org/10.1155/2022/1926797DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9217550PMC
July 2022

Turn-off detection of Cr(III) with chelation enhanced fluorescence quenching effect by a naphthyl hydrazone Shiff base chemosensor.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Nov 6;281:121599. Epub 2022 Jul 6.

School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address:

A thiophene substituted naphthyl hydrazone derivative NHT was synthesized using a one-step route for the detection of trivalent chromium (Cr). UV-visible absorption and emission spectra, density functional theory calculations as well as H NMR titration confirmed that the probe underwent a turn-off response via the chelation enhanced fluorescence quenching effect upon exposure to Cr and the NHT-Cr complex was formed at a 1:1 binding stoichiometry. NHT exhibited a fast response rate of 2.3 min in buffer solution and a relatively low limit of detection of 41 nM. In addition, the Schiff base chemosensor exhibited excellent selectivity with high affinity towards Cr in the presence of other competing cations. Bioimaging of the probe in PC3 cells further demonstrated the potential real life application of the probe in detecting Cr.
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http://dx.doi.org/10.1016/j.saa.2022.121599DOI Listing
November 2022

Preparation of efficient adsorbent with dual adsorption function based on semi-coke: Adsorption properties and mechanisms.

J Colloid Interface Sci 2022 Jul 2;626:674-686. Epub 2022 Jul 2.

Key Laboratory of Clay Mineral Applied Research of Gansu Province, Center of Eco-material and Green Chemistry, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China. Electronic address:

Solid waste oil shale semi-coke (SC) is considered as a potential material for the preparation of adsorbents due to its rich organic and inorganic components. However, the dense structure and the complex form of the components present make it unsatisfactory for adsorption. In this study, using Alkali corrosion under hydrothermal condition, SC was prepared as an adsorbent material PSSC with excellent adsorption performance for both anionic and cationic dyes. The related characterization analysis showed that most of the organic matter and silicates of SC reacted with inorganic matter to form silicon carbide. The specific surface area of PSSC increased significantly (from 7.47 m/g to 1353.99 m/g) leading to the maximum adsorption of PSSC on Methylene blue (MB) and Congo red (CR) reached at 974.66 mg/g and 562.41 mg/g, respectively, which far exceeded the adsorption of SC on both dyes. Finally, the adsorption mechanisms were revealed from the structural and energetic perspectives. The results showed that the modification process increased the type and number of adsorption sites, and that these adsorption sites exhibited different capturing abilities for the two dyes with the change of contact temperature.
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http://dx.doi.org/10.1016/j.jcis.2022.06.100DOI Listing
July 2022

[Evaluation of Mercury Pollution in Soil of Different Land Use Types in Coal-fired Industrial Area].

Huan Jing Ke Xue 2022 Jul;43(7):3781-3788

Research Center for Scientific Development in Fenhe River Valley, Taiyuan Normal University, Jinzhong 030619, China.

The geo-accumulation index, potential ecological risk index, health risk model, and geographical detector were used to evaluate the soil mercury (Hg) concentrations and assess the health risks for various land use types to reveal the characteristics and spatial variability of soil Hg pollution under different land uses in the coal-fired industrial area of the Loess Plateau. The study area included seven counties in the western part of Xinzhou City, Shanxi province. The results showed that the average (Hg) in the agricultural land, grass land, wood land, and residential land were 0.48, 0.34, 0.58, and 0.52 mg·kg, respectively, which were 16.00, 11.33, 19.33, and 17.33 times higher than the background value, respectively. The variation coefficients were ranked as residential land>agricultural land>grass land>wood land. The evaluation results of the geo-accumulation index and potential ecological risk index indicated a high ecological risk of Hg pollution in the entire study area with extreme pollution points in certain aeras. The health risk assessment results indicated no non-carcinogenic health risks for children and adults in the study area from Hg. Additionally, the geographical detection results revealed that the spatial variability in Hg was mainly impacted by industrial and agricultural activities in agricultural land, by soil organic matter and pH value in grass land, by soil salinity in wood land, and by soil salinity and organic matter in residential land. The spatial distribution pattern of Hg concentrations peaked in both the northern and southern edges, with a decreasing trend towards the middle and the majorly polluted areas being caused by human activities such as coal mining and industrial production.
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http://dx.doi.org/10.13227/j.hjkx.202109061DOI Listing
July 2022

Influence of coronal-morphology of endplate and intervertebral space to cage subsidence and fusion following oblique lumbar interbody fusion.

BMC Musculoskelet Disord 2022 Jul 4;23(1):633. Epub 2022 Jul 4.

Department of Orthopedics, Orthopedic Research Institute, West China Hospital, Sichuan University, 37# Wuhou Guoxue road, Chengdu, 610041, China.

Background: Endplate morphology is considered to be one of the influencing factors of cage subsidence after lumbar interbody fusion (LIF). Previous radiographic evaluations on the endplate mostly used sagittal X-ray or MRI. However, there are few studies on the CT evaluation of the endplate and intervertebral space (IVS), especially the evaluation of coronal morphology and its influence on subsidence and fusion after LIF. We aimed to measure and classify the shapes of the endplate and IVS using coronal CT imaging and evaluate the radiographic and clinical outcomes of different shapes of the endplate/IVS following oblique lateral lumbar interbody fusion (OLIF).

Methods: A total of 137 patients (average age 59.1 years, including 75 males and 62 females) who underwent L4-5 OLIF combined with anterolateral fixation from June 2018 to June 2020 were included. The endplate concavity depth (ECD) was measured on the preoperative coronal CT image. According to ECD, the endplate was classified as flat (< 2 mm), shallow (2-4 mm), or deep (> 4 mm). The L4-5 IVS was further classified according to endplate type. The disc height (DH), DH changes, subsidence rate, fusion rate, and Oswestry Disability Index (ODI) in different endplate/IVS shapes were evaluated during 1-year follow up.

Results: The ECD of L4 inferior endplate (IEP) was significantly deeper than that of L5 superior endplate (SEP) (4.2 ± 1.1 vs 1.6 ± 0.8, P < 0.01). Four types of L4-5 IVS were identified: shallow-shallow (16, 11.7%), shallow-flat (45, 32.9%), deep-shallow (32, 23.4%), and deep-flat (44, 32.1%). A total of 45 (32.9%) cases of cage subsidence were observed. Only one (6.3%) subsidence event occurred in the shallow-shallow group, which was significantly lower than in the other three groups (19 shallow-flat, 6 deep-shallow, and 19 deep-flat) (P < 0.05). Meanwhile, the shallow-shallow group had the highest fusion rate (15, 93.8%) and the highest rate of reach minimal clinically important difference (MCID) ODI among the four types. For a single endplate, the shape of L4 IEP is the main influencing factor of the final interbody fusion rate, and the shallow shape L4 IEP facilitates fusion ( OR = 2.85, p = 0.03). On the other hand, the flat shape L5 SEP was the main risk factor to cage subsidence (OR = 4.36, p < 0.01).

Conclusion: The L4-5 IVS is asymmetrical on coronal CT view and tends to be fornix-above and flat-down. The shallow-shallow IVS has the lowest subsidence rate and best fusion result, which is possibly because it has a relatively good degree in matching either the upper or lower interface of the cage and endplates. These findings provide a basis for the further improvements in the design of OLIF cages.
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http://dx.doi.org/10.1186/s12891-022-05584-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252057PMC
July 2022

Degradation of Organic Dyes Using the Ionizing Irradiation Process in the Presence of the CN/CD/Fe Composite: Mechanistic Studies.

ACS Omega 2022 Jun 10;7(25):21418-21432. Epub 2022 Jun 10.

School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.

Organic dyes are ubiquitous pollutants in various aquatic environments as they are produced in abundance and used widely. In the present work, the degradation and mineralization of various organic dyes such as methylene blue (MB), methyl orange (MO), and rhodamine B (RhB), following the electron beam irradiation method in the presence of a graphitic carbon nitride/carbon nanodots/Fe(II) (CN/CD/Fe) composite, were studied. The removal efficiency of MB reached 81.7% under conditions of electron beam irradiation (EBI) when the total irradiation dose was 5 kGy. This increased to 91.2% in the presence of the CN/CD/Fe composite. The mineralization efficiency increased from 30.1 to 47.3% when the composite was added, and the total irradiation dose was 20 kGy. The removal efficiency of organic dyes was not significantly affected in the pH range of 3-11. Results from cyclic experiments conducted using MB degradation indicated that the CN/CD/Fe composite exhibited good stability and reusability even after five irradiation cycles. Results from scavenging experiments revealed that OH was the predominant reactive species during the MB degradation process. Intermediates produced in the synergistic system (EBI&CN/CD/Fe system) consisting of the CN/CD/Fe composite and EBI were detected using the liquid chromatography-mass spectrometry (LC-MS) technique. Based on the results, the possible degradation mechanism and pathways for MB were proposed.
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http://dx.doi.org/10.1021/acsomega.2c00512DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9244913PMC
June 2022

The autotetraploid potato genome provides insights into highly heterozygous species.

Plant Biotechnol J 2022 Jun 29. Epub 2022 Jun 29.

Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, China.

Potato (Solanum tuberosum L.) originated in the Andes and evolved its vegetative propagation strategy through short day-dependent tuber development. Herein, we present a high-quality, chromosome-scale reference genome sequence of a tetraploid potato cultivar. The total length of this genome assembly was 2.67 Gb, with scaffold N50 and contig N50 sizes of 46.24 and 2.19 Mb, respectively. In total, 1.69 Gb repetitive sequences were obtained through de novo annotation, and long terminal repeats were the main transposable elements. A total of 126 070 protein-coding genes were annotated, of which 125 077 (99.21%) were located on chromosomes. The 48 chromosomes were classified into four haplotypes. We annotated 31 506 homologous genes, including 5913 (18.77%) genes with four homologues, 11 103 (35.24%) with three homologues, 12 177 (38.65%) with two homologues and 2313 (7.34%) with one homologue. MLH3, MSH6/7 and RFC3, which are the genes involved in the mismatch repair pathway, were found to be significantly expanded in the tetraploid potato genome relative to the diploid potato genome. Genome-wide association analysis revealed that cytochrome P450, flavonoid synthesis, chalcone enzyme, glycosyl hydrolase and glycosyl transferase genes were significantly correlated with the flesh colours of potato tuber in 150 tetraploid potatoes. This study provides valuable insights into the highly heterozygous autotetraploid potato genome and may facilitate the development of tools for potato cultivar breeding and further studies on autotetraploid crops.
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http://dx.doi.org/10.1111/pbi.13883DOI Listing
June 2022

A1-40 Oligomers Trigger Neutrophil Extracellular Trap Formation through TLR4- and NADPH Oxidase-Dependent Pathways in Age-Related Macular Degeneration.

Oxid Med Cell Longev 2022 18;2022:6489923. Epub 2022 Jun 18.

Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Neutrophils participate in the advancement of the human innate immune system and respond to perceived endogenous and exogenous threats. As a response mechanism, neutrophil extracellular traps (NETs) form near pathogens and surrounding tissues during an immune response. Drusen is an important marker of Age-Related Macular Degeneration (AMD) and plays an important role in the course of AMD. A1-40 is the main component of drusen. However, the relationship between NETs and AMD or A1-40 is unclear. Here, we found elevated levels of NETs in the serum of AMD patients and elevated levels in the serum of mouse models. We also observed the accumulation of neutrophils in the mouse retina. In addition, the production of NETs was inhibited by PAD4 inhibitors, which can alleviate chronic inflammation. Moreover, we confirmed that A1-40 can induce NETs formation via the Toll-like receptor 4 (TLR4) and neutrophil NADPH oxidase (NOX) pathways. Our study confirmed that the formation of NETs is induced by A1-40, and the results suggest that NETs may play a vital role in AMD pathogenesis.
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http://dx.doi.org/10.1155/2022/6489923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233592PMC
June 2022

Out-of-Domain Generalization From a Single Source: An Uncertainty Quantification Approach.

IEEE Trans Pattern Anal Mach Intell 2022 Jun 20;PP. Epub 2022 Jun 20.

We are concerned with a worst-case scenario in model generalization, in the sense that a model aims to perform well on many unseen domains while there is only one single domain available for training. We propose Meta-Learning based Adversarial Domain Augmentation to solve this Out-of-Domain generalization problem. The key idea is to leverage adversarial training to create "fictitious" yet "challenging" populations, from which a model can learn to generalize with theoretical guarantees. To facilitate fast and desirable domain augmentation, we cast the model training in a meta-learning scheme and use a Wasserstein Auto-Encoder to relax the widely used worst-case constraint. We further improve our method by integrating uncertainty quantification for efficient domain generalization. Extensive experiments on multiple benchmark datasets indicate its superior performance in tackling single domain generalization.
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http://dx.doi.org/10.1109/TPAMI.2022.3184598DOI Listing
June 2022

NiCoPd Inlaid NiCo-Bimetallene for Efficient Electrocatalytic Methanol Oxidation.

Inorg Chem 2022 Jul 18;61(26):10211-10219. Epub 2022 Jun 18.

Faculty of Engineering and Environment, Northumbria University, Newcastle upon Tyne NE1 8ST, U.K.

Pd-based metallenes have attracted great attention recently as newly burgeoning two-dimensional (2D) materials, attributed to their significantly increased active surface areas and intrinsic electrocatalytic activities. Therefore, they could be used as a potential candidate as the high-performance electrocatalyst for methanol oxidation reactions (MORs) in the direct methanol fuel cell. Herein, a new strategy is proposed to fabricate NiCoPd inlaid NiCo-bimetallene (NiCoPd/NiCo-bimetallene) by the structure directing effect of 18-crown-6 ether under an ultrasonic-pulse interface together with the HCHO reduction and atom-diffusion-aging process. NiCoPd ternary-alloys with uniformly dispersed Pd active sites are decorated onto NiCo-bimetallenes, achieving remarkably enhancing the effective utilization of Pd atoms. What is more, the intrinsic activity is enhanced by the "bifunctional mechanism" of NiCo-bimetallene adsorption of intermediate species and increased Pd-active sites. Moreover, the anti-CO poisoning ability is optimized through the "alloying ligand effect" of NiCoPd. Therefore, the NiCoPd/NiCo-bimetallene exhibits excellent mass activity for MOR, which is higher than commercial Pd/C. This work suggests a new way of the Pd-based metallenes catalyst approach to the efficient electrocatalytic MOR.
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http://dx.doi.org/10.1021/acs.inorgchem.2c01534DOI Listing
July 2022

Tilianin Reduces Apoptosis the ERK/EGR1/BCL2L1 Pathway in Ischemia/Reperfusion-Induced Acute Kidney Injury Mice.

Front Pharmacol 2022 3;13:862584. Epub 2022 Jun 3.

Department of Nephrology, the Affiliated Hospital of Qingdao University, Qingdao, China.

Acute kidney injury (AKI) is a common syndrome impacting about 13.3 million patients per year. Tilianin has been reported to alleviate myocardial ischemia/reperfusion (I/R) injury, while its effect on AKI is unknown; thus, this study aimed to explore if tilianin protects I/R-induced AKI and the underlying mechanisms. The microarray dataset GSE52004 was downloaded from GEO DataSets (Gene Expression Omnibus). Differential expression analysis and gene-set enrichment analysis (GSEA) were performed by R software to identify apoptosis pathway-related genes. Then, was applied to identify the transcription factor (TF) related to apoptosis. The STRING database was used to construct a protein-protein interaction (PPI) network. Cytoscape software visualized PPI networks, and hub TFs were selected via AutoDock was used for molecular docking of tilianin and hub gene-encoded proteins. The expression levels of hub genes were assayed and visualized by quantitative real-time PCR, Western blotting, and immunohistochemistry by establishing I/R-induced AKI mouse models. Bioinformatics analysis showed that 34 genes, including FOS, ATF4, and Gadd45g, were involved in the apoptosis pathway. In total, seven hub TFs might play important roles in tilianin-regulating apoptosis pathways. In , tilianin improved kidney function and reduced the number of TUNEL-positive renal tubular epithelial cells (RTECs) after I/R-induced AKI. Tilianin reduced the activation of the ERK pathway and then downregulated the expression of EGR1. This further ameliorated the expression of anti-apoptotic genes such as BCL2L1 and BCL2, reduced pro-apoptotic genes such as BAD, BAX, and caspase-3, and reduced the release of cytochrome c. Tilianin reduced apoptosis after I/R-induced AKI by the ERK/EGR1/BCL2L1 pathway. Our findings provided novel insights for the first time into the protective effect and underlying molecular mechanisms of tilianin on I/R-induced AKI.
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http://dx.doi.org/10.3389/fphar.2022.862584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204490PMC
June 2022

A tin porphyrin axially-coordinated two-dimensional covalent organic polymer for efficient hydrogen evolution.

Chem Commun (Camb) 2022 Jun 30;58(53):7423-7426. Epub 2022 Jun 30.

School of Chemistry & Chemical Engineering, Jiangsu University, Zhenjiang 212013, P. R. China.

Herein, we demonstrate a facile strategy for constructing an efficient and stable hydrogen evolution reaction (HER) catalyst, a tin porphyrin axially-coordinated 2D covalent organic polymer (SnTPPCOP). SnTPPCOP exhibits promising HER activity with a low overpotential of 147 mV at 10 mA cm due to its unique structural properties, ranking among the best records reported recently.
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http://dx.doi.org/10.1039/d2cc02775eDOI Listing
June 2022

[Scutellarin alleviates lipopolysaccharide-induced renal injury via mediating cysteine-rich protein 61-connective tissue growth factor-nephroblastoma overexpressed gene 1 expression to inhibit nuclear factor-κB signaling pathway].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2022 Apr;34(4):400-406

Department of Nephrology, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China.

Objective: To explore the protective effect and mechanism of scutellarin (Scu) on sepsis associated-acute kidney injury (SA-AKI).

Methods: (1) In vivo experiment: 36 male C57BL/6 mice were divided into normal saline (NS) control group, lipopolysaccharide (LPS) induced SA-AKI model group (LPS group), 20 mg/kg Scu control group (Scu 20 control group), and 5, 10, 20 mg/kg Scu pretreatment groups by random number table with 6 mice in each group. The SA-AKI model was reproduced by intraperitoneal injection of 10 mg/kg LPS. The NS control group was injected with NS intraperitoneally. The Scu pretreatment groups were intraperitoneally injected with different doses of Scu every day before LPS injection for 1 week. Scu 20 control group was injected with 20 mg/kg Scu for 1 week. After 24 hours of LPS treatment, mice in each group were sacrificed, kidney tissues were collected, and kidney injury was detected by hematoxylin-eosin (HE) staining. Western blotting was used to detect the protein expression levels of nuclear factor-κB (NF-κB) signaling pathway related molecules, apoptosis-related proteins and cysteine-rich protein 61-connective tissue growth factor-nephroblastoma overexpressed gene 1 (CCN1). (2) In vitro experiment: human renal tubular epithelial cell line HK-2 was cultured in vitro and used for experiment when the cells fused to 80%. In the cells without LPS treatment and after 100 g/L LPS treatment, pcDNA3.1-CCN1 and small interfering RNA (siRNA) CCN1 sequence were transfected to overexpress and inhibit CCN1 expression, respectively, to observe whether CCN1 was involved in NF-κB signaling pathway activation and apoptosis. In addition, 100g/L LPS and 20 μmol/L Scu were added into HK-2 cells transfected with and without CCN1 siRNA to investigate the mechanism of protective effect of Scu on LPS-induced HK-2 cells injury.

Results: (1) The results of in vivo experiment: the renal function of SA-AKI mice induced by LPS was significantly decreased, and had kidney histological damage and severely damaged renal tubules. Scu could alleviate renal function and histological damage in a dose-dependent manner. Western blotting results showed Scu could reduce the protein expression of NF-κB signaling pathway related molecules and CCN1 in the renal tissue, and had a significant alleviating effect on apoptosis, indicating that CCN1 was involved in NF-κB signaling pathway activation and apoptosis. (2) The results of in vitro experiment: in HK-2 cells not treated with LPS, CCN1 overexpression had no effect on apoptosis related protein and pro-inflammatory factors of NF-κB signaling pathway. In HK-2 cells treated with LPS, overexpression of CCN1 significantly inhibited the mRNA expressions of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and monocyte chemoattractant protein-1 (MCP-1), with significant differences as compared with cells stimulated only by LPS [IL-1β mRNA (2): 3.20±0.57 vs. 4.88±0.69, TNF-α mRNA (2): 2.99±0.44 vs. 5.00±0.81, MCP-1 mRNA (2): 2.81±0.50 vs. 5.41±0.75, all P < 0.05], and the apoptosis-related protein was significantly down-regulated. However, when siRNA was used to inhibit the expression of CCN1, the mRNA expressions of pro-inflammatory factors were significantly increased as compared with cells stimulated only by LPS [IL-1β mRNA (2): 6.01±1.13 vs. 4.88±0.69, TNF-α mRNA (2): 5.15±0.86 vs. 5.00±0.81, all P < 0.05], and apoptosis-related protein was significantly up-regulated. In the LPS-induced HK-2 cells, the mRNA expressions of pro-inflammatory factors were significantly down-regulated after Scu treatment as compared with cells stimulated only by LPS [IL-1β mRNA(2): 2.55±0.50 vs. 6.15±1.04, TNF-α mRNA (2): 2.58±0.40 vs. 3.95±0.52, MCP-1 mRNA (2): 2.64±0.44 vs. 6.21±0.96, all P < 0.05], and apoptosis-related protein was also significantly reduced. When the expression of CCN1 was inhibited by siRNA, the protective effect of Scu on cells was weakened, which showed that the mRNA expressions of pro-inflammatory factors in cells was significantly up-regulated compared with the cells without inhibition of CCN1 expression [IL-1β mRNA (2): 5.34±0.76 vs. 2.55±0.50, TNF-α mRNA (2): 3.66±0.54 vs. 2.58±0.40, MCP-1 mRNA (2): 5.15±0.79 vs. 2.64±0.44, all P < 0.05], and the expression of apoptosis related protein was also significantly up-regulated.

Conclusions: Scu could protect the renal function in SA-AKI mice, and the protective effect is associated with NF-κB signaling pathway and CCN1. Thus, Scu could alleviate LPS-induced kidney injury by regulating the NF-κB signaling pathway.
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http://dx.doi.org/10.3760/cma.j.cn121430-20210401-00767DOI Listing
April 2022

Necrostatin-1 decreases necroptosis and inflammatory markers after intraventricular hemorrhage in mice.

Neural Regen Res 2022 Dec;17(12):2710-2716

Department of Neurosurgery, West China Medical School, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China.

Necrostatin-1, an inhibitor of necroptosis, can effectively inhibit necrotic apoptosis in neurological diseases, which results in the inhibition of inflammation, endoplasmic reticulum stress, and reactive oxygen species production and substantial improvement of neurological function. However, the effects of necrostatin-1 on intraventricular hemorrhage (IVH) remain unknown. In this study, we established a mouse model of IVH by injecting autologous blood into the lateral ventricle of the brain. We also injected necrostatin-1 into the lateral ventricle one hour prior to IVH induction. We found that necrostatin-1 effectively reduced the expression levels of the necroptosis markers receptor-interacting protein kinase (RIP)1, RIP3, mixed lineage kinase domain-like protein (MLKL), phosphorylated (p)-RIP3, and p-MLKL and the levels of interleukin-1β , interleukin-6, and tumor necrosis factor-α in the surrounding areas of the lateral ventricle. However, necrostatin-1 did not reduce ependymal ciliary injury or brain water content. These findings suggest that necrostatin-1 can prevent local inflammation and microglial activation induced by IVH but does not greatly improve prognosis.
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http://dx.doi.org/10.4103/1673-5374.339488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9165399PMC
December 2022

Comparison of Pb and Cd in wheat grains under air-soil-wheat system near lead-zinc smelters and total suspended particulate introduced modeling attempt.

Sci Total Environ 2022 Sep 27;839:156290. Epub 2022 May 27.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environment Sciences, Beijing 100012, China.

The excessive accumulation of wheat grain metals and metalloids caused by ambient air contamination has drawn an increasing concern. However, at present, the differences in the pathways of cadmium and lead accumulation in wheat grains in an air-soil-wheat system are not clear. In this study, wheat was grown around a lead‑zinc smelting area and exposed to different soil Pb and Cd levels and different ambient air Pb and Cd levels. Lead and Cd accumulation in wheat grains was examined in this study. Two models of wheat grain Pb and Cd concentrations were established based on the 3 variables including soil Pb and Cd concentration, ambient air Pb and Cd concentration, and soil pH. The results showed that total suspended particulate (TSP), soil, and wheat grains exhibited different degrees of Pb and Cd contamination in the study area, and the contamination of Cd is more serious than Pb contamination. The Pb in wheat grains was more likely to derive from ambient air than from soil, whereas the impact of ambient air on the accumulation of Cd in wheat grains might be very limited. This speculation was confirmed by the results of the predictor variable relative weight method based on the multiple regression analysis. Introduction of ambient air factor (TSP Pb and Cd) greatly improved the modeling effect of wheat grains Pb, while the modeling of grain Cd was more dependent on soil pH and total soil Cd. This research suggests that the reduction in wheat grain Pb is likely to be achieved by the control over ambient air Pb, whereas the reduction in the wheat grain Cd by the remediation of soil pollutants.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156290DOI Listing
September 2022

Scutellarin protects the kidney from ischemia/reperfusion injury by targeting Nrf2.

Nephrology (Carlton) 2022 Aug 15;27(8):690-700. Epub 2022 Jun 15.

Department of Nephrology, The Affiliated Hospital of Qingdao University, Qingdao, China.

Background: Acute kidney injury (AKI) results in high morbidity and mortality among inpatients, while effective treatment and intervention are still absent. Therefore, this study aims to explore the effects of Scutellarin (Scu) in experimental models in vivo and in vitro.

Methods: In vivo experiment, we employed a total of 30 Wistar rats, which further were modelled by a bilateral renal pedicle clip for 45 min, then received 50 mg/kg/day Scu. In vitro, HK-2 cells were administered with 20μΜ Scu and then incubated in hypoxia/reoxygenation (H/R) conditions for 24 h. The knockdown of Nrf2 expression was conducted by small interfering RNA (siRNA) transfection.

Results: As a result, the AKI model was well established with an increased SCr, BUN, KIM-1 level, and histological injury score, while Scu treatment reduced the levels above and increased the antioxidative enzyme HO-1. H/R induced an increase of ROS in HK-2 cells, while Scu decreased the ROS level. Bioinformatics results showed the transcription factor Nrf2 was a hub protein during the AKI, which also bound to Scu with low binding energy, indicating that the downstream effect of Scu might be mediated by Nrf2. To verify the suppose above, we employed siRNA against Nrf2, which shows a significant increase in ROS after Nrf2 was blocked. Meanwhile, the HO-1 showed similar expression compared with the 'H/R + Nrf2 siRNA' and 'H/R + Nrf2 siRNA + Scu' group, implying the protective effect of Scu was mediated by the Nrf2/HO-1 pathway.

Conclusion: Scu led to up-regulation of HO-1 through activating the Nrf2 signalling pathway, protecting the kidneys from ischemia/reperfusion (I/R)-induced oxidative damage.
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http://dx.doi.org/10.1111/nep.14069DOI Listing
August 2022

Ixazomib-Associated Skin Exfoliation.

Mediterr J Hematol Infect Dis 2022 1;14(1):e2022045. Epub 2022 May 1.

Department of Hematology, First Hospital of Lanzhou University, Lanzhou, China.

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http://dx.doi.org/10.4084/MJHID.2022.045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083939PMC
May 2022

Effect of repetitive transcranial magnetic stimulation in inducing weight loss in patients with chronic schizophrenia: a randomized, double-blind controlled 4-week study.

Curr Neuropharmacol 2022 May 24. Epub 2022 May 24.

Peking University HuiLongGuan Clinical Medical School, Beijing HuiLongGuan Hospital, Beijing, China.

Objectives: There is emerging evidence that high-frequency (HF) repetitive transcranial magnetic stimulation (rTMS) may promote weight loss in individuals with obesity in general population. However, so far, no such a study has been carried out in patients with schizophrenia (SZ). This study was designed to evaluate the efficacy of 10Hz rTMS in reducing body weight in patients with chronic SZ.

Methods: Forty-seven SZ patients were randomly assigned to two groups: 10Hz rTMS (n=27) or sham stimulation (n=20) over DLPFC (applied once daily) for 20 consecutive treatments. Body weight was assessed at baseline, at the end of week 1, week 2, week 3 and week 4. Clinical symptoms were evaluated with the Positive and Negative Syndrome Scale (PANSS) at baseline and at the end of week 4.

Results: We found that compared with patients in sham group, 10Hz rTMS treatment significantly reduced body weight in patients with chronic SZ after a period of 4 weeks of stimulation. Interestingly, further analysis found that from the first week (5 sessions) of treatment, there was a significant difference in body weight between active and sham groups after controlling for baseline weight. However, active rTMS treatment did not improve the psychotic symptoms compared to sham stimulation.

Conclusion: Our results suggest that add-on HF rTMS could be an effective therapeutic strategy for body weight control in patients with chronic SZ.
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http://dx.doi.org/10.2174/1570159X20666220524123315DOI Listing
May 2022
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