Publications by authors named "Long Wang"

1,108 Publications

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Chemotherapy-induced peripheral neuropathy is promoted by enhanced spinal insulin-like growth factor-1 levels via astrocyte-dependent mechanisms.

Brain Res Bull 2021 Jul 29. Epub 2021 Jul 29.

Department of Anesthesiology, The First People's Hospital of Foshan, Foshan 528000, Guangdong, China. Electronic address:

Background: Chemotherapy-induced peripheral neuropathy (CIPN) is a common and intractable complication in chemotherapy-receiving patients. Insulin-like growth factor-1 (IGF-1) is a popular neurotrophin with various functions, such as maintaining neuronal survival and synaptic functioning in the central nervous system. Therefore, we hypothesized that the IGF-1 signaling pathway could be a candidate target for treating CIPN.

Methods: We established the CIPN model by injecting mice intraperitoneally with oxaliplatin and assessed IGF-1 protein expression, its receptor IGF1R, phospho-IGF1R (p-IGF1R), interleukin-17A (IL-17A), tumor necrosis factor-α (TNF-α), and calcitonin gene-related peptide (CGRP) in the lumbar spinal cord with Western blot and immunofluorescence. To examine the effect of IGF-1 signaling on CIPN, we injected mice intrathecally or intraperitoneally with mouse recombinant IGF-1 (rIGF-1).

Results: IGF-1 protein expression decreased significantly in the spinal cord on D3 and D10 (the 3rd and 10th days after beginning oxaliplatin chemotherapy) and was co-localized with astrocytes primarily in the lumbar spinal cord, whereas IGF1R was predominantly expressed on neurons. Both intrathecally- and intraperitoneally-administered rIGF-1 relieved the chemotherapy-induced pain-like behavior and reduced IL-17A, TNF-α, and CGRP protein expressions in the spinal cord.

Conclusion: Our results indicate a vital role for IGF-1 signaling in CIPN. Targeting IGF-1 signaling could be a potent therapeutic strategy for treating CIPN in clinical settings.
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http://dx.doi.org/10.1016/j.brainresbull.2021.07.026DOI Listing
July 2021

Comparison of Two Genomes From Different Clades Reveals Independent Evolution of Alpha-Amylase Duplication, Variation in Secondary Metabolism Genes, and Differences in Primary Metabolism.

Front Microbiol 2021 13;12:691296. Epub 2021 Jul 13.

Molecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, MA, United States.

Microbes (bacteria, yeasts, molds), in addition to plants and animals, were domesticated for their roles in food preservation, nutrition and flavor. is a domesticated filamentous fungal species traditionally used during fermentation of Asian foods and beverage, such as sake, soy sauce, and miso. To date, little is known about the extent of genome and phenotypic variation of isolates from different clades. Here, we used long-read Oxford Nanopore and short-read Illumina sequencing to produce a highly accurate and contiguous genome assemble of 14160, an industrial strain from China. To understand the relationship of this isolate, we performed phylogenetic analysis with 90 isolates and 1 isolate of the progenitor, . This analysis showed that 14160 is a member of clade A, in comparison to the RIB 40 type strain, which is a member of clade F. To explore genome variation between isolates from distinct clades, we compared the 14160 genome with the complete RIB 40 genome. Our results provide evidence of independent evolution of the alpha-amylase gene duplication, which is one of the major adaptive mutations resulting from domestication. Synteny analysis revealed that both genomes have three copies of the alpha-amylase gene, but only one copy on chromosome 2 was conserved. While the RIB 40 genome had additional copies of the alpha-amylase gene on chromosomes III, and V, 14160 had a second copy on chromosome II and an third copy on chromosome VI. Additionally, we identified hundreds of lineage specific genes, and putative high impact mutations in genes involved in secondary metabolism, including several of the core biosynthetic genes. Finally, to examine the functional effects of genome variation between strains, we measured amylase activity, proteolytic activity, and growth rate on several different substrates. RIB 40 produced significantly higher levels of amylase compared to 14160 when grown on rice and starch. Accordingly, RIB 40 grew faster on rice, while 14160 grew faster on soy. Taken together, our analyses reveal substantial genome and phenotypic variation within .
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http://dx.doi.org/10.3389/fmicb.2021.691296DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313989PMC
July 2021

Elderly Woman With Chest Tightness.

Authors:
Jun Huang Long Wang

Ann Emerg Med 2021 Aug;78(2):230-286

Department of Radiology, the Affiliated Changzhou No.2 People's Hospital with Nanjing Medical University, Changzhou, 213003, China.

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http://dx.doi.org/10.1016/j.annemergmed.2021.02.016DOI Listing
August 2021

The role of sleep disorders in cardiovascular diseases: Culprit or accomplice?

Life Sci 2021 Jul 26:119851. Epub 2021 Jul 26.

Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China. Electronic address:

Sleep disorders frequently comorbid with several cardiovascular diseases (CVDs), attracting increasing scientific attention and interest. Sleep disorders include insomnia, sleep-disordered breathing, restless legs syndrome, etc. It is well known that inflammation, sympathetic activation, and endothelial dysfunction play critical roles in sleep disorders, all of which are predisposing factors for CVDs. The comorbidity of sleep disorders and CVDs may have a bidirectional relationship. Patients with CVDs may have a high incidence of sleep disorders and vice versa. This review focused on the comorbidity of sleep disorders and CVDs and discussed the potential pathophysiological mechanisms and therapeutic strategies. In addition to the existing mechanisms, this review summarized novel potential mechanisms underlying comorbidities, such as gut microbiota, orexin, and extracellular vesicles, which may provide a theoretical basis for further basic research and clinical investigations on improving therapeutic outcomes.
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http://dx.doi.org/10.1016/j.lfs.2021.119851DOI Listing
July 2021

Simulated operation combined with patient-specific instrumentation technology is superior to conventional technology for supramalleolar osteotomy: a retrospective comparative study.

Am J Transl Res 2021 15;13(6):6087-6097. Epub 2021 Jun 15.

Department of Orthopedics, Xiangya Hospital Central South University No. 87 Xiangya Road, Changsha 410008, Hunan, China.

Objective: Over the past seven years, our team has designed a simulated operation combined with patient-specific instrumentation (SO-PSI) assisted supramalleolar osteotomy (SMOT) method and applied it in the clinic. This study aimed to evaluate the differences between SO-PSI technology and conventional operation (CO) technology for SMOT in preoperative planning, intraoperative application, and postoperative curative effect.

Methods: We retrospectively analyzed SMOT data collected from our hospital between October 2014 and December 2018. Patients (n = 28) were enrolled and divided into CO (n = 17) and SO-PSI (n = 11) groups; mean follow-up time was 33.4 (range, 13 to 59) months. We statistically analyzed and compared perioperative data, accuracy of preoperative planning, intraoperative application, difference between pre- and post-operative radiologic ankle angles, changes in American Orthopaedic Foot & Ankle Society (AOFAS) score, visual analogue scale (VAS) score, range of ankle motion, and Takakura stage after surgery.

Results: All ankle alignments and positions were recovered for both groups. Compared with the CO group, the SO-PSI group had a shorter mean operating time and postoperative hospital stay, a decreased number of fluoroscopy examinations, lower albumin reduction, longer preoperative planning time and preoperative hospital stay, and increased hospitalization expenses. In the SO-PSI group, comparison of ankle angles at preoperative planning and postoperatively revealed good correlation, while this was not the case in the CO group. Mean tibial ankle center discrepancy for the SO-PSI group was 1.86 ± 1.06 mm. On follow-up, all radiologic parameters for the two groups improved significantly; however, the improvement of the tibial anterior surface angle and tibiotalar tilt angle for the SO-PSI group were more obvious than those for the CO group. AOFAS score, VAS score, ankle range of motion, and Takakura stage improved after surgery in both groups; however, the improvements in the SO-PSI group were greater than those in the CO group overall.

Conclusions: SO-PSI technology can facilitate accurate and rapid preoperative planning for SMOT. In general, compared with conventional technology, SO-PSI has advantages for preoperative planning, intraoperative application, and postoperative curative effect.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290754PMC
June 2021

Petroleum oil and products recovery from oily sludge: Characterization and analysis of pyrolysis products.

Environ Res 2021 Jul 16;202:111675. Epub 2021 Jul 16.

Beijing Advanced Innovation Center for Soft Matter Science and Engineering, State Key Laboratory of Organic-Inorganic Composites, College of Chemical Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.

Oily sludge (OS) has attracted special interest because of its hazardous nature and high potential as an energy resource. This study investigated the oil recovery from OS by thermal cracking and catalytic pyrolysis. The oil yield increased when the temperature exceeded 450 °C and reached a maximum (76.84 wt%) at 750 °C. Catalysts significantly improved the quality of oil produced during catalytic pyrolysis. Aromatic hydrocarbons were dominant (10.01-52.69%) in pyrolysis oil (PO) from OS catalytic pyrolysis, and the catalysts significantly reduced the presence of oxygen heterocycles. In addition, KOH and CaO reduced the I (D-band peak intensity)/I (G-band peak intensity) of OS char (OC) and increased the degree of graphitization. Owing to its higher iodine adsorption value and methylene blue (MB) adsorption value, OC exhibits potential as an adsorbent. The environmental assessment and potential applications of OC, along with possible reaction mechanisms and kinetic characteristics, are also discussed.
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http://dx.doi.org/10.1016/j.envres.2021.111675DOI Listing
July 2021

Microwave-assisted pyrolysis of oily sludge from offshore oilfield for recovery of high-quality products.

J Hazard Mater 2021 Jul 3;420:126578. Epub 2021 Jul 3.

Tianjin Chemical Research & Design Institute, China National Offshore Oil Corporation, Tianjin 300131, China.

Microwave pyrolysis of oily sludge (OS) was investigated in this study. In this case, the highest oil yield (85.93 wt%) was achieved at 500 °C. The molar ratio of H/C was lower for OS char (OC) at higher pyrolysis temperatures, indicating good stability of OC owing to high degree of carbonization and aromaticity. Then, iodine adsorption value of OC reached maximum (531.2 mg/g) at 750 °C. While methylene blue (MB) uptake slightly increased with temperature and reached maximum (384.08 mg/g) at 850 °C. In order to improve the quality of pyrolysis products, different catalysts were employed in OS pyrolysis. The maximum content (64.31%) of aromatic hydrocarbon was found in PO500-10β. In addition, β-zeolite also reduced oxygenates content in oil, beneficial for stability of oil products. The gas products from catalytic pyrolysis were abundant in CO and CH, and KOH achieved the highest CO (5.9 wt%), CH (16.9 wt%) and H (2.4 wt%) yields. Finally, a reaction mechanism pathway for OS pyrolysis was proposed to show the production routes of gas, liquid, and solid products.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126578DOI Listing
July 2021

Clinical efficacy of closed reduction and percutaneous parallel K-wire interlocking fixation of first metacarpal base fracture.

J Orthop Surg Res 2021 Jul 14;16(1):454. Epub 2021 Jul 14.

Department of Orthopedics, Xiangya Hospital, Central South University (Hunan Engineering Research Center of Biomedical Metal and Ceramic Implants), No. 87 Xiangya Road, Changsha, 410008, Hunan, China.

Background: This study aimed to explore the clinical efficacy of treating a first metacarpal base fracture by closed reduction and percutaneous parallel K-wire interlocking fixation between the first and second metacarpals.

Methods: Twenty patients treated by the abovementioned modified technique (modified technique group) and ten patients treated by the traditional percutaneous K-wire fixation technique (traditional technique group) from October 2015 to November 2019 at our institution were retrospectively reviewed. The patients' average age was 38 years (range, 16-61 years). The mean follow-up period was 13 months (range, 10-18 months). At the final follow-up, the functional recovery of the injured hand was assessed and compared between the modified and traditional technique groups. In addition, the functional recovery of the injured hand was compared with that of the uninjured hand within the modified technique group.

Results: All patients recovered well, with no cases of infection or nonunion. Compared with the traditional technique group, the modified technique group had a shorter operative time, lower postoperative visual analogue scale pain score, better effective range of motion score of the first carpometacarpal joint (Kapandji score), and had almost no need for auxiliary plaster fixation, enabling functional exercise to be started earlier. Within the modified technique group, the mean hand grip strength, pinch strength, and Kapandji score on the injured side did not significantly differ to the values on the uninjured side in both the extra-articular and intra-articular fracture subgroups. While the abduction and flexion-extension arcs of the thumb on the injured hand were significantly smaller than those on the uninjured hand in both the extra-articular and intra-articular fracture subgroups, the patients felt clinically well with respect to daily activities and strength.

Conclusion: The percutaneous parallel K-wire and interlocking fixation technique is simple, effective, and economical for first metacarpal base fractures.
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http://dx.doi.org/10.1186/s13018-021-02600-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278589PMC
July 2021

Feasibility of cortical bone trajectory screws for bridging fixation in revision surgery for lumbar adjacent segment degeneration.

Medicine (Baltimore) 2021 Jul;100(28):e26666

Department of Orthopedics, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, The Key Laboratory of Digital Orthopedics of Yunnan Province, No. 157 Jinbi Road, Kunming, China.

Background: To investigate the feasibility of using cortical bone trajectory (CBT) screws for bridging fixation in revision surgery for lumbar adjacent segment degeneration and to provide a reference for clinical practice.

Methods: Computed tomography scans of the lumbar spines of 36 patients in our hospital were used. Sixteen males and 20 females with an average age of 65.5 ± 10.5 years (range: 46 to 83 years) were included. Three-dimensional reconstruction was performed using computer software. Screws with appropriate sizes were selected for the L1 to L5 vertebral segments, and traditional pedicle screws were placed using the standard method. After completing screw placement, simulated placement of CBT screws was performed separately. No overlap occurred between the two screws in the process of CBT screw placement, and the placement point and direction were adjusted until screw placement completion. After all screw placement simulations were complete, according to the contact area of the cortical bone of the screw trajectory and the screw puncture position and distance through the trajectory, the screw placement results were categorized as excellent, good, general, and failure. Excellent and good ratings were considered successful, while a general rating was regarded as acceptable. Then, the success rate and acceptable rate of each segment of the lumbar spine were calculated.

Results: Three hundred and sixty screw placement simulations were performed in lumbar pedicles, and 72 CBT screws were implanted in each vertebral body of the lumbar spine. The success rates in the L1 to L5 segments were 73.6%, 80.6%, 83.3%, 88.9%, and 77.8%, respectively, and the acceptable rates were 91.7%, 97.2%, 97.2%, 100%, and 91.7%, respectively. The overall success rate and acceptable rate of CBT screw placement in the lumbar spine were 80.8% and 95.6%, respectively.

Conclusion: CBT screws are feasible for bridging fixation in lumbar adjacent segment degeneration revision surgery, and the accuracy of screw placement in different lumbar vertebrae varies.
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http://dx.doi.org/10.1097/MD.0000000000026666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284764PMC
July 2021

Inhibition of Death-associated Protein Kinase 1 protects against Epileptic Seizures in mice.

Int J Biol Sci 2021 11;17(9):2356-2366. Epub 2021 Jun 11.

Fujian Key Laboratory for Translational Research in Cancer and Neurodegenerative Diseases, Institute for Translational Medicine, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350122, China.

Epilepsy is a chronic encephalopathy and one of the most common neurological disorders. Death-associated protein kinase 1 (DAPK1) expression has been shown to be upregulated in the brains of human epilepsy patients compared with those of normal subjects. However, little is known about the impact of DAPK1 on epileptic seizure conditions. In this study, we aim to clarify whether and how DAPK1 is regulated in epilepsy and whether targeting DAPK1 expression or activity has a protective effect against epilepsy using seizure animal models. Here, we found that cortical and hippocampal DAPK1 activity but not DAPK1 expression was increased immediately after convulsive pentylenetetrazol (PTZ) exposure in mice. However, DAPK1 overexpression was found after chronic low-dose PTZ insults during the kindling paradigm. The suppression of DAPK1 expression by genetic knockout significantly reduced PTZ-induced seizure phenotypes and the development of kindled seizures. Moreover, pharmacological inhibition of DAPK1 activity exerted rapid antiepileptic effects in both acute and chronic epilepsy mouse models. Mechanistically, PTZ stimulated the phosphorylation of NR2B through DAPK1 activation. Combined together, these results suggest that DAPK1 regulation is a novel mechanism for the control of both acute and chronic epilepsy and provide new therapeutic strategies for the treatment of human epilepsy.
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http://dx.doi.org/10.7150/ijbs.59922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241737PMC
June 2021

Whole transcriptome analysis resulted in the identification of Chinese sprangletop (Leptochloa chinensis) genes involved in cyhalofop-butyl tolerance.

BMC Genomics 2021 Jul 9;22(1):521. Epub 2021 Jul 9.

Longping Branch, Graduate School of Hunan University, Changsha, People's Republic of China.

Background: Chinese sprangletop [Leptochloa chinensis (L.) Nees] is an annual malignant weed, which can often be found in paddy fields. Cyhalofop-butyl is a specialized herbicide which is utilized to control L. chinensis. However, in many areas, L. chinensis has become tolerant to this key herbicide due to its continuous long-term use.

Results: In this study, we utilized a tolerant (LC18002) and a sensitive (LC17041) L. chinensis populations previously identified in our laboratory, which were divided into four different groups. We then employed whole transcriptome analysis to identify candidate genes which may be involved in cyhalofop-butyl tolerance. This analysis resulted in the identification of six possible candidate genes, including three cytochrome P450 genes and three ATP-binding cassette transporter genes. We then carried out a phylogenetic analysis to identify homologs of the differentially expressed cytochrome P450 genes. This phylogenetic analysis indicated that all genes have close homologs in other species, some of which have been implicated in non-target site resistance (NTSR).

Conclusions: This study is the first to use whole transcriptome analysis to identify herbicide non-target resistance genes in L. chinensis. The differentially expressed genes represent promising targets for better understanding herbicide tolerance in L. chinensis. The six genes belonging to classes already associated in herbicide tolerance may play important roles in the metabolic resistance of L. chinensis to cyhalofop-butyl, although the exact mechanisms require further study.
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http://dx.doi.org/10.1186/s12864-021-07856-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268407PMC
July 2021

Relationship of Bone Status with Serum Uric Acid and Bilirubin in Men with Type 2 Diabetes: A Cross-Sectional Study.

Med Sci Monit 2021 Jun 18;27:e930410. Epub 2021 Jun 18.

Department of Endocrinology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China (mainland).

BACKGROUND Accumulating evidence has shown that serum uric acid and bilirubin are associated with some chronic diseases, owing to their antioxidant capacity, but the previous research produced discrepant results regarding the relation between uric acid, as well as bilirubin, and bone health. This study was designed to assess the relationship of serum uric acid and total bilirubin with bone mineral density and bone turnover markers in men with type 2 diabetes. MATERIAL AND METHODS In total, 631 male patients with type 2 diabetes were included. Data of patients' medical history, biochemical index, bone mineral density of the lumbar vertebra, femoral neck, and total hip, and bone turnover markers including osteocalcin (OC), amino-terminal propeptide of type I procollagen (PINP), type I collagen carboxy-terminal peptide (CTX), and parathyroid hormone were collected and retrospectively analyzed. RESULTS Both serum uric acid and total bilirubin were positively related to bone mineral density of the lumbar vertebra (b=0.179, p.
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http://dx.doi.org/10.12659/MSM.930410DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218604PMC
June 2021

Evolution of state-dependent strategies in stochastic games.

J Theor Biol 2021 Oct 25;527:110818. Epub 2021 Jun 25.

Center for Systems and Control, College of Engineering, Peking University, Beijing 100871, China; Center for Multi-Agent Research, Institute for Artificial Intelligence, Peking University, Beijing 100871, China. Electronic address:

In a population of interacting individuals, the environment for interactions often changes due to individuals' behaviors, which in turn drive the evolution of individuals' behaviors. The interplay between the environment and individuals' behaviors has been demonstrated to remarkably influence the evolutionary outcomes. In reality, in highly cognitive species such as social primates and human beings, individuals are often capable of perceiving the environment change and then differentiate their strategies across different environment states. We propose a model of environmental feedback with state-dependent strategies: individuals have perceptions of distinct environment states and therefore take distinct sub-strategies under each of them; based on the sub-strategy, individuals then decide their behaviors; their behaviors subsequently modify the environment state. We use the theory of stochastic games and evolutionary dynamics to analyze this idea. We find that when environment changes slower than behaviors, state-dependent strategies (i.e. taking different sub-strategies under different environment states) can outperform state-independent strategies (i.e. taking an identical sub-strategy under all environment states), such as Win-Stay, Lose-Shift, the most leading strategy among state-independent strategies. The intuition is that delayed environmental feedback provides chances for individuals with state-dependent strategies to exploit those with state-independent strategies. Our results hold (1) in both well-mixed and structured populations; (2) when the environment switches between two or more states. Furthermore, the environment changing rate decides if state-dependent strategies benefit global cooperation. The evolution sees the rise of the cooperation level for fast environment switching and the decrease otherwise. Our work stresses that individuals' perceptions of different environment states are beneficial to their survival and social prosperity in a changing world.
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http://dx.doi.org/10.1016/j.jtbi.2021.110818DOI Listing
October 2021

Isoflurane Alleviates Myocardial Injury Induced by Hypoxia/Reoxygenation by Regulating miR-18a-5p.

Cardiovasc Toxicol 2021 Jun 28. Epub 2021 Jun 28.

Department of Anesthesiology, The First Hospital of Lanzhou University, No. 1, Donggang West Road, Lanzhou, 730000, China.

The protective effect and mechanism of isoflurane on myocardial injury was investigated by constructing in vitro hypoxia/reoxygenation (HR) cell model. HR cell models were established in vitro and treated with isoflurane (ISO). qRT-PCR was used to detect the relative expression of miR-18a-5p. CCK-8 kit and flow cytometry were performed to evaluate cell proliferation and apoptosis. The myocardial injury related markers, such as Cκ-MB, cTnI and LDH were detected by ELISA. Luciferase reporter gene assay was used to verify the interaction between miR-18a-5p and target genes. The expression of miR-18a-5p was significantly increased in hypoxic cardiomyocytes compared with control group (P < 0.001). Meanwhile, cardiomyocytes in the HR group showed inhibition of proliferation, a significant increase in cell apoptosis and in myocardial injury indicators, such as Cκ-MB, cTnI and LDH (P < 0.001). However, 1% ISO treatment alleviated myocardial cell injury induced by HR. Transfection of miR-18a-5p under ISO reduced the protective effect of 1% ISO against myocardial cell damage. Luciferase report gene assay confirmed that CCND2 might be the target gene of miR-18a-5p. In the in vitro cell model of myocardium, ISO alleviated cardiomyocyte injury caused by hypoxia/reoxygenation by down-regulating the expression of miR-18a-5p.
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http://dx.doi.org/10.1007/s12012-021-09670-1DOI Listing
June 2021

AMF Inoculation Can Enhance Yield of Transgenic Maize and Its Control Efficiency Against Especially Under Elevated CO.

Front Plant Sci 2021 8;12:655060. Epub 2021 Jun 8.

Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.

The promotion and application of transgenic crops provides an approach for the prevention and control of target lepidopteran pests and effectively relieves the environmental pressure caused by the massive usage of chemical pesticides in fields. However, studies have shown that crops will face a new risk due to a decrease in exogenous toxin content under elevated carbon dioxide (CO) concentration, thus negatively affecting the ecological sustainability of crops. Arbuscular mycorrhizal fungi (AMF) are important beneficial microorganisms that can effectively improve the nutrient status of host plants and are expected to relieve the ecological risk of crops under increasing CO due to global climate change. In this study, the maize and its parental line of non-transgenic maize were selected and inoculated with a species of AMF (, synonyms: ), in order to study the secondary defensive chemicals and yield of maize, and to explore the effects of inoculation on the growth, development, and reproduction of the pest fed on maize and non- maize under ambient carbon dioxide concentration (CO) and elevated carbon dioxide concentration (CO). The results showed that CO increased the AM fungal colonization, maize yield, and foliar contents of jasmonic acid (JA) and salicylic acid (SA), but decreased foliar Bt toxin content and gene expression in maize leaves. inoculation increased maize yield, foliar JA, SA contents, toxin contents, and gene expression in maize leaves, and positively improved the growth, development, reproduction, and food utilization of the fed on non- maize. However, inoculation was unfavorable for the fitness of fed on maize, and the effect was intensified when combined with CO. It is indicated that inoculation had adverse effects on the production of non- maize due to the high potential risk of population occurrence of , while it was just the opposite for maize. Therefore, this study confirms that the AMF can increase the yield and promote the expression levels of its endogenous (JA, SA) and exogenous (Bt toxin) secondary defense substances of maize under CO, and finally can enhance the insect resistance capacity of crops, which will help ensure the sustainable utilization and safety of crops under climate change.
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http://dx.doi.org/10.3389/fpls.2021.655060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217876PMC
June 2021

Man With Left Knee Mass.

Ann Emerg Med 2021 07;78(1):e1-e2

Department of Radiology, the Affiliated Changzhou No. 2 People's Hospital with Nanjing Medical University, Changzhou, China.

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http://dx.doi.org/10.1016/j.annemergmed.2021.01.007DOI Listing
July 2021

Fe-doped chrysotile nanotubes containing siRNAs to silence SPAG5 to treat bladder cancer.

J Nanobiotechnology 2021 Jun 23;19(1):189. Epub 2021 Jun 23.

Department of Oncology, The Third Xiangya Hospital of Central South University, No.138, Tongzipo Road, Changsha, 410013, Hunan, China.

Background: For certain human cancers, sperm associated antigen 5 (SPAG5) exerts important functions for their development and progression. However, whether RNA interference (RNAi) targeting SPAG5 has antitumor effects has not been determined clinically.

Results: The results indicated that Fe-doped chrysotile nanotubes (FeSiNTs) with a relatively uniform outer diameter (15-25 nm) and inner diameter (7-8 nm), and a length of several hundred nanometers, which delivered an siRNA against the SPAG5 oncogene (siSPAG5) efficiently. The nanomaterials were designed to prolong the half-life of siSPAG5 in blood, increase tumor cell-specific uptake, and maximize the efficiency of SPAG5 silencing. In vitro, FeSiNTs carrying siSPAG5 inhibited the growth, migration, and invasion of bladder cancer cells. In vivo, the FeSiNTs inhibited growth and metastasis in three models of bladder tumors (a tail vein injection lung metastatic model, an in-situ bladder cancer model, and a subcutaneous model) with no obvious toxicities. Mechanistically, we showed that FeSiNTs/siSPAG5 repressed PI3K/AKT/mTOR signaling, which suppressed the growth and progression of tumor cells.

Conclusions: The results highlight that FeSiNTs/siSPAG5 caused no activation of the innate immune response nor any systemic toxicity, indicating the possible therapeutic utility of FeSiNTs/siSPAG5 to deliver siSPAG5 to treat bladder cancer.
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http://dx.doi.org/10.1186/s12951-021-00935-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220725PMC
June 2021

The complete chloroplast genome of a Chinese medicinal plant, (Thunb.) Bremek. (Lamiales: Acanthaceae) from Nanjing, China.

Mitochondrial DNA B Resour 2021 Jun 7;6(7):1888-1889. Epub 2021 Jun 7.

School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, China.

(Thunb.) Bremek. is a widely distributed medicinal plant species in China and Japan. In this study, the complete chloroplast genome sequence of was assembled and characterized from high-throughput sequencing data. The chloroplast genome is 151,374 bp in length, consisting of a large single-copy (LSC) and a small single-copy (SSC) regions of 83,395 bp and 17,073 bp, respectively, which were separated by a pair of 25,453 bp inverted repeat (IR) regions. The overall GC content of the genome is 38.07%. The genome contains 133 genes, including 88 protein-coding, 37 tRNA, and eight rRNA genes. A phylogenetic tree reconstructed using 23 chloroplast genomes reveals that form a separate group which is a sister of the genus . The work reported here is the first complete chloroplast genome of which will provide useful information to the evolutionary studies on the genus of .
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http://dx.doi.org/10.1080/23802359.2021.1934157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189061PMC
June 2021

Decreased Resting-State Functional Connectivity of Periaqueductal Gray in Temporal Lobe Epilepsy Comorbid With Migraine.

Front Neurol 2021 26;12:636202. Epub 2021 May 26.

Department of Neurology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Patients with temporal lobe epilepsy (TLE) are at high risk for having a comorbid condition of migraine, and these two common diseases are proposed to have some shared pathophysiological mechanisms. Our recent study indicated the dysfunction of periaqueductal gray (PAG), a key pain-modulating structure, contributes to the development of pain hypersensitivity and epileptogenesis in epilepsy. This study is to investigate the functional connectivity of PAG network in epilepsy comorbid with migraine. Thirty-two patients with TLE, including 16 epilepsy patients without migraine (EwoM) and 16 epilepsy patients with comorbid migraine (EwM), and 14 matched healthy controls (HCs) were recruited and underwent resting functional magnetic resonance imaging (fMRI) scans to measure the resting-state functional connectivity (RsFC) of PAG network. The frequency and severity of migraine attacks were assessed using the Migraine Disability Assessment Questionnaire (MIDAS) and Visual Analog Scale/Score (VAS). In animal experiments, FluoroGold (FG), a retrograde tracing agent, was injected into PPN and its fluorescence detected in vlPAG to trace the neuronal projection from vlPAG to PPN. FG traced neuron number was used to evaluate the neural transmission activity of vlPAG-PPN pathway. The data were processed and analyzed using DPARSF and SPSS17.0 software. Based on the RsFC finding, the excitatory transmission of PAG and the associated brain structure was studied via retrograde tracing in combination with immunohistochemical labeling of excitatory neurons. Compared to HCs group, the RsFC between PAG and the left pedunculopontine nucleus (PPN), between PAG and the corpus callosum (CC), was decreased both in EwoM and EwM group, while the RsFC between PAG and the right PPN was increased only in EwoM group but not in EwM group. Compared to EwoM group, the RsFC between PAG and the right PPN was decreased in EwM group. Furthermore, the RsFC between PAG and PPN was negatively correlated with the frequency and severity of migraine attacks. In animal study, a seizure stimulation induced excitatory transmission from PAG to PPN was decreased in rats with chronic epilepsy as compared to that in normal control rats. The comorbidity of epilepsy and migraine is associated with the decreased RsFC between PAG and PPN.
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http://dx.doi.org/10.3389/fneur.2021.636202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189422PMC
May 2021

Chromosomal-level genome assembly of the semi-dwarf rice Taichung Native 1, an initiator of Green Revolution.

Genomics 2021 Jul 7;113(4):2656-2674. Epub 2021 Jun 7.

Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 300, Taiwan; Biodiversity Research Center, Academia Sinica, Taipei 115, Taiwan; Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637, USA. Electronic address:

Here we report the 409.5 Mb chromosome-level assembly of the first bred semi-dwarf rice, the Taichung Native 1 (TN1), which served as the template for the development of the Green Revolution (GR) cultivar IR8 "miracle rice". We sequenced the TN1 genome utilizing multiple platforms and produced PacBio long reads, Illumina paired-end reads, Illumina mate-pair reads and 10x Genomics linked reads. We used a hybrid approach to assemble the 226× coverage of sequences by a combination of de novo and reference-guided approaches. The assembled TN1 genome has an N50 scaffold size of 33.1 Mb with the longest measuring 45.5 Mb. We annotated 37,526 genes, in which 24,102 (64.23%) were assigned Blast2GO annotations. The genome has 4672 or 95.4% complete BUSCOs and a repeat content of 51.52%. We developed our own method of creating a GR pangenome using the orthologous relationships of the proteins of TN1, IR8, MH63 and IR64, identifying 16,999 core orthologue groups of Green Revolution. From the pangenome, we identified a set of shared and unique gene ontology terms for the accessory clusters, characterizing TN1, IR8, MH63 and IR64. This TN1 genome assembly and GR pangenome will be a resource for new genomic discoveries about Green Revolution, and for improving the disease and insect resistances and the yield of rice.
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http://dx.doi.org/10.1016/j.ygeno.2021.06.006DOI Listing
July 2021

The Role of Regulatory B cells in Kidney Diseases.

Front Immunol 2021 24;12:683926. Epub 2021 May 24.

Department of Kidney Transplantation, The Second Xiangya Hospital of Central South University, Changsha, China.

B cells, commonly regarded as proinflammatory antibody-producing cells, are detrimental to individuals with autoimmune diseases. However, in recent years, several studies have shown that regulatory B (Breg) cells, an immunosuppressive subset of B cells, may exert protective effects against autoimmune diseases by secretion of inhibitory cytokines such as IL-10. In practice, Breg cells are identified by their production of immune-regulatory cytokines, such as IL-10, TGF-β, and IL-35, however, no specific marker or Breg cell-specific transcription factor has been identified. Multiple phenotypes of Breg cells have been found, whose functions vary according to their phenotype. This review summarizes the discovery, phenotypes, development, and function of Breg cells and highlights their potential therapeutic value in kidney diseases.
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http://dx.doi.org/10.3389/fimmu.2021.683926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183681PMC
May 2021

A Novel Phage Infecting Represents a Distinct Group of Siphophages Infecting Diverse Aquatic Copiotrophs.

mSphere 2021 Jun 9;6(3):e0045421. Epub 2021 Jun 9.

State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Institute of Marine Microbes and Ecospheres, Xiamen University, Xiamen, China.

Bacteriophages play critical roles in impacting microbial community succession both ecologically and evolutionarily. Although the majority of phage genetic diversity has been increasingly unveiled, phages infecting members of the ecologically important genus remain poorly understood. Here, we present a comprehensive analysis of a newly isolated alterophage, vB_AcoS-R7M (R7M), to characterize its life cycle traits, genomic features, and putative evolutionary origin. R7M harbors abundant genes identified as host-like auxiliary metabolic genes facilitating viral propagation. Genomic analysis suggested that R7M is distinct from currently known alterophages. Interestingly, R7M was found to share a set of similar characteristics with a number of siphophages infecting diverse aquatic opportunistic copiotrophs. We therefore proposed the creation of one new subfamily () to group with these evolutionarily related phages. Notably, tail genes were less likely to be shared among them, and baseplate-related genes varied the most. In-depth analyses indicated that R7M has replaced its distal tail with a Rhodobacter capsulatus gene transfer agent (RcGTA)-like baseplate and further acquired a putative receptor interaction site targeting . These findings suggest that horizontal exchanges of viral tail adsorption apparatuses are widespread and vital for phages to hunt new hosts and to adapt to new niches. The evolution and ecology of phages infecting members of , a marine opportunistic genus that is widely distributed and of great ecological significance, remain poorly understood. The present study integrates physiological and genomic evidence to characterize the properties and putative phage-host interactions of a newly isolated phage, vB_AcoS-R7M (R7M). A taxonomic study reveals close evolutionary relationships among R7M and a number of siphophages infecting various aquatic copiotrophs. Their similar head morphology and overall genetic framework suggest their putative common ancestry and the grouping of a new viral subfamily. However, their major difference lies in the viral tail adsorption apparatuses and the horizontal exchanges of which possibly account for variations in host specificity. These findings outline an evolutionary scenario for the emergence of diverse viral lineages of a shared genetic pool and give insights into the genetics and ecology of viral host jumps.
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http://dx.doi.org/10.1128/mSphere.00454-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8265664PMC
June 2021

The gut microbiome: implications for neurogenesis and neurological diseases.

Neural Regen Res 2022 Jan;17(1):53-58

Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China.

There is an increasing recognition of the strong links between the gut microbiome and the brain, and there is persuasive evidence that the gut microbiome plays a role in a variety of physiological processes in the central nervous system. This review summarizes findings that gut microbial composition alterations are linked to hippocampal neurogenesis, as well as the possible mechanisms of action; the existing literature suggests that microbiota influence neurogenic processes, which can result in neurological disorders. We consider this evidence from the perspectives of neuroinflammation, microbial-derived metabolites, neurotrophins, and neurotransmitters. Based on the existing research, we propose that the administration of probiotics can normalize the gut microbiome. This could therefore also represent a promising treatment strategy to counteract neurological impairment.
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http://dx.doi.org/10.4103/1673-5374.315227DOI Listing
January 2022

A Hybrid Rao-NM Algorithm for Image Template Matching.

Entropy (Basel) 2021 May 27;23(6). Epub 2021 May 27.

School of Computer and Communication Engineering, University of Science and Technology Beijing, Beijing 100083, China.

This paper proposes a hybrid Rao-Nelder-Mead (Rao-NM) algorithm for image template matching is proposed. The developed algorithm incorporates the Rao-1 algorithm and NM algorithm serially. Thus, the powerful global search capability of the Rao-1 algorithm and local search capability of NM algorithm is fully exploited. It can quickly and accurately search for the high-quality optimal solution on the basis of ensuring global convergence. The computing time is highly reduced, while the matching accuracy is significantly improved. Four commonly applied optimization problems and three image datasets are employed to assess the performance of the proposed method. Meanwhile, three commonly used algorithms, including generic Rao-1 algorithm, particle swarm optimization (PSO), genetic algorithm (GA), are considered as benchmarking algorithms. The experiment results demonstrate that the proposed method is effective and efficient in solving image matching problems.
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http://dx.doi.org/10.3390/e23060678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229128PMC
May 2021

Aspiration dynamics generate robust predictions in heterogeneous populations.

Nat Commun 2021 05 31;12(1):3250. Epub 2021 May 31.

Center for Systems and Control, College of Engineering, Peking University, Beijing, China.

Update rules, which describe how individuals adjust their behavior over time, affect the outcome of social interactions. Theoretical studies have shown that evolutionary outcomes are sensitive to model details when update rules are imitation-based but are robust when update rules are self-evaluation based. However, studies of self-evaluation based rules have focused on homogeneous population structures where each individual has the same number of neighbors. Here, we consider heterogeneous population structures represented by weighted networks. Under weak selection, we analytically derive the condition for strategy success, which coincides with the classical condition of risk-dominance. This condition holds for all weighted networks and distributions of aspiration levels, and for individualized ways of self-evaluation. Our findings recover previous results as special cases and demonstrate the universality of the robustness property under self-evaluation based rules. Our work thus sheds light on the intrinsic difference between evolutionary dynamics under self-evaluation based and imitation-based update rules.
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http://dx.doi.org/10.1038/s41467-021-23548-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166829PMC
May 2021

Woman With Fever and Chest Pain.

Ann Emerg Med 2021 06;77(6):e113-e114

Department of Radiology, The Affiliated Changzhou No.2 People's Hospital with Nanjing Medical University, Changzhou, Jiangsu Province, China.

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http://dx.doi.org/10.1016/j.annemergmed.2020.12.009DOI Listing
June 2021

Nigrumol A, a new triterpenoid from subsp. (Nakai ex H.Ito) Kuvaev.

Nat Prod Res 2021 May 25:1-5. Epub 2021 May 25.

Inner Mongolia Research Institute of Traditional Mongolian Medicine Engineering Technology, Tongliao, China.

A new triterpenoid, named nigrumol A (), along with 5 known triterpenoids were isolated from the aerial parts of subsp. (Nakai ex H.Ito) Kuvaev () . The structure of was elucidated by analysis of its spectroscopic data, including UV, IR, HR-ESI-MS and extensive 1 D and 2 D NMR techniques. Compound showed that it could decrease ethanol-induced or CCl4-induced L02 cell toxicity effectively.
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http://dx.doi.org/10.1080/14786419.2021.1929975DOI Listing
May 2021

Distal Arterialized Venous Supercharging Improves Perfusion and Survival in an Extended Dorsal Three-Perforasome Perforator Flap Rat Model.

Plast Reconstr Surg 2021 Jun;147(6):957e-966e

From the Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University; Zhejiang Provincial Key Laboratory of Orthopaedics; and The Second Clinical Medical College of Wenzhou Medical University.

Background: Perforator flaps are commonly applied for a variety of skin defects. Many strategies (e.g., hyperbaric oxygen and preconditioning) have been investigated to improve flap survival, but a postoperative 2.03 to 18.2 percent flap necrosis frequency remains a major complication. The authors hypothesized that a distal arterialized venous supercharged (DAVS) flap procedure might improve perfusion and survival in an extended three-perforasome perforator flap rat model and rescue flap ischemia intraoperatively.

Methods: One hundred twenty male Sprague-Dawley rats (200 to 300 g) were divided into the thoracodorsal artery (TDA) flap group and the DAVS flap group (n = 60 per group). An approximately 11 × 2.5-cm2 flap based on the TDA perforasome was designed in the TDA flap. A DAVS flap was designed based on the TDA flap and supercharged by anastomosing the rat caudal artery with the deep circumflex iliac vein. At postoperative times 1, 3, 6, and 12 hours and 1, 3, 5, and 7 days, perfusion and angiography were compared. On day 7, flap viability and angiogenesis were assessed using histology and Western blotting.

Results: The DAVS flap showed a higher survival rate compared with the TDA flap (100 percent versus 81.93 ± 5.38 percent; p < 0.001). All blood flow ratios of deep circumflex iliac artery to TDA perforasome and of choke zone II to choke zone I were higher in the DAVS flap (all p < 0.05). Angiography qualitatively revealed that choke vessels in choke zone II dilated earlier and extensively in the DAVS flap group. CD34+ vessels (68.66 ± 12.53/mm2 versus 36.82 ± 8.99/mm2; p < 0.001) and vascular endothelial growth factor protein level (0.22 ± 0.03 versus 0.11 ± 0.03; p < 0.001) were significantly increased in the DAVS flap group.

Conclusions: The DAVS procedure improves three-perforasome perforator flap survival and can be used for rescuing flap ischemia intraoperatively. Further study is needed before possible clinical adoption for reconstructive operations.
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http://dx.doi.org/10.1097/PRS.0000000000007990DOI Listing
June 2021

The quality of life of patients with pacemaker-induced cardiomyopathy after they upgrade to left bundle branch pacing.

Am J Transl Res 2021 15;13(4):3044-3053. Epub 2021 Apr 15.

Department of Cardiovascular Medicine, Qingyang Second People's Hospital Qingyang, Gansu Province, China.

Objective: To investigate the changes in the cardiac function and quality of life of patients with pacemaker-induced cardiomyopathy (PICM) after they upgrade to left bundle branch pacing (LBBP).

Methods: Ten patients with PICM who upgraded to LBBP were recruited as the study cohort. The LBBP upgrade operations were all performed by the same cardiac intervention expert. The cardiac function index changes, including the left ventricular end-diastolic diameters, the cardiothoracic ratios, the left ventricular ejection fractions, the N-terminal brain natriuretic peptide levels, the 6 min walking test distances, and the quality of life changes, including the SF-36 scores, the European Five-Dimensional Health Scale (EQ-5D-3L) scores, and the Minnesota Heart failure Quality of Life Scale (MLHFQ) scores before and after the LBBP operations were analyzed. The incidences of adverse events during the postoperative follow-up were analyzed.

Results: The ten patients were successfully upgraded to LBBP. Compared with before the operation, the left ventricular end-diastolic diameter levels and the cardiothoracic ratios decreased significantly, but the left ventricular ejection fractions and the 6 min walking test distances increased (P<0.05 or P<0.01). The SF-36 and EQ-5D-3L scores increased gradually, but the MLHFQ scores decreased gradually before the operations and at one month and 12 months after the operations. The physical functioning (PF), role-physical (RP), general health (GH), vitality (VT), and the social role (SR) scores of the SF-36 scale, the TTO index and the visual analog scale (VAS) of the EQ-5D-3L scale scores, and the MLHFQ scores at 12 months after the operations were significantly different from the scores before the operations (all P<0.05). In terms of safety, there were no adverse events such as pacemaker electrode dislocation or interventricular septal perforation during the postoperative follow-up.

Conclusion: Upgrading to LBBP can effectively improve cardiac function and the quality of life in patients with PICM, and the operation is safe.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129225PMC
April 2021

Development and phylogenetic utilities of a new set of single-/low-copy nuclear genes in Senecioneae (Asteraceae), with new insights into the tribal position and the relationships within subtribe Tussilagininae.

Mol Phylogenet Evol 2021 Sep 14;162:107202. Epub 2021 May 14.

Department of Botany, National Museum of Natural History, MRC 166, Smithsonian Institution, Washington, DC 20013-7012, USA. Electronic address:

The tribe Senecioneae is one of the largest tribes in Asteraceae, with a nearly cosmopolitan distribution. Despite great efforts devoted to elucidate the evolution of Senecioneae, many questions still remain concerning the systematics of this group, from the tribal circumscription and position to species relationships in many genera. The hybridization-based target enrichment method of next-generation sequencing has been accepted as a promising approach to resolve phylogenetic problems. We herein develop a set of single-/low-copy genes for Senecioneae, and test their phylogenetic utilities. Our results demonstrate that these genes work highly efficiently for Senecioneae, with a high average gene recovery of 98.8% across the tribe and recovering robust phylogenetic hypotheses at different levels. In particular, the delimitation of the Senecioneae has been confirmed to include Abrotanella and exclude Doronicum, with the former sister to core Senecioneae and the latter shown to be more closely related to Calenduleae. Moreover, Doronicum and Calenduleae are inferred to be the closest relatives of Senecioneae, which is a new hypothesis well supported by statistical topology tests, morphological evidence, and the profile of pyrrolizidine alkaloids, a special kind of chemical characters generally used to define Senecioneae. Furthermore, this study suggests a complex reticulation history in the diversification of Senecioneae, accounting for the prevalence of polyploid groups in the tribe. With subtribe Tussilagininae s.str. as a case study showing a more evident pattern of gene duplication, we further explored reconstructing the phylogeny in the groups with high ploidy levels. Our results also demonstrate that tree topologies based on sorted paralogous copies are stable across different methods of phylogenetic inference, and more congruent with the morphological evidence and the results of previous phylogenetic studies.
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http://dx.doi.org/10.1016/j.ympev.2021.107202DOI Listing
September 2021
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