Publications by authors named "Long Ma"

478 Publications

A Review on Basalt Fiber Composites and Their Applications in Clean Energy Sector and Power Grids.

Polymers (Basel) 2022 Jun 12;14(12). Epub 2022 Jun 12.

Hebei Key Laboratory of Distributed Energy Storage and Micro-Grid, North China Electric Power University, Yonghua North Street No. 619, Baoding 071003, China.

Basalt fiber (BF) has a high mechanical strength, excellent temperature resistance, good chemical stability, low energy consumption, and an environmentally friendly production process. In addition, BF-reinforced polymers (BFRPs) have good corrosion resistance and designability; thus, they meet the application requirements of electrical equipment, such as new conductors, insulating pull rods, and composite cross-arms. However, there are still a series of technical issues in the mass production of BF, and the stability of the products needs to be further improved. Therefore, the research on the production, modification, and application of BF is necessary. This paper discusses the chemical composition and production technology of BF, describes the morphology and properties of BF, summarizes the interface problems and modification methods of composites, and finally, introduces the application prospects of BF in the field of electrical materials, which is expected to provide a reference for the application and promotion of BFRP in the future.
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http://dx.doi.org/10.3390/polym14122376DOI Listing
June 2022

[Temporal and spatial variations of annual precipitation and meteorological drought in China during 1951-2018].

Ying Yong Sheng Tai Xue Bao 2022 Jun;33(6):1572-1580

College of Water Conservancy and Civil Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.

Exploring the temporal and spatial variations of precipitation and drought is an important topic in hydro-logy. Based on the precipitation data of 619 meteorological stations in China from 1951 to 2018, we used anomaly percentage method and Morlet wavelet analysis to analyze the temporal and spatial variations of annual precipitation and drought. The results showed that annual precipitation in China showed a stepwise decreasing trend from southeast to northwest during the study period, and that the intensity of annual precipitation change was on the contrary. The precipitation near the boundary of the second and third steps showed a downward trend, and the abrupt change of precipitation occurred mainly in the 1960s and 1970s. The rest region was on the rise, with substantial changes in the 1990s. The main period of precipitation was short in the regions with temperate continental climate and temperate monsoon climate. From 1960s to 2010s, the area of arid land in China had decreased, while that of the semi-arid area and semi-humid area had increased gradually, especially in the recent decade. An aridity boundary was found between 30° N and 40° N, with drought frequency in its north being much more than the south. On the whole, the frequency and scope of drought events showed a decreasing trend and its interdecadal shift direction was from the central part of northwest China to the southern part of North China and then to the northern part of North China.
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http://dx.doi.org/10.13287/j.1001-9332.202206.022DOI Listing
June 2022

First case report of complete paternal isodisomy of chromosome 10 harbouring a novel variant in COL17A1 that causes junctional epidermolysis bullosa intermediate.

BMC Med Genomics 2022 Jun 18;15(1):136. Epub 2022 Jun 18.

Center for Reproductive Medicine, Changhai Hospital, Naval Medical University, Shanghai, China.

Background: Uniparental disomy (UPD) is a condition in which both chromosomes are inherited from the same parent, except for imprinting disorders. Uniparental isodisomy (UPiD) may result in a homozygous variant contributing to an autosomal recessive disorder in the offspring of a heterozygous carrier. Junctional epidermolysis bullosa intermediate (JEB intermediate) is an autosomal recessive inherited disease that is associated with a series of gene variants, including those of COL17A1.

Case Presentation: We report the first case of complete paternal UPiD of chromosome 10 harbouring a novel homozygous variant in COL17A1: c.1880(exon23)delG (p.G627Afs*56). This variant led to the clinical phenotype of junctional epidermolysis bullosa intermediate in a 5-year-old child. Trio-whole exome sequencing (Trio-WES) and in silico data analysis were used for variant identification, Sanger sequencing was performed for variant validation, and pathological examination was performed as the gold standard for phenotype confirmation.

Conclusions: We recommend the use of WES as a first-tier test for the diagnosis of epidermolysis bullosa, especially for paediatric patients. Moreover, UPD events should be detected and analysed routinely through WES data in the future.
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http://dx.doi.org/10.1186/s12920-022-01285-xDOI Listing
June 2022

Effective Charged Exterior Surfaces for Enhanced Ionic Diffusion through Nanopores under Salt Gradients.

J Phys Chem Lett 2022 Jun 16;13(24):5669-5676. Epub 2022 Jun 16.

Key Laboratory of High Efficiency and Clean Mechanical Manufacture of Ministry of Education, National Demonstration Center for Experimental Mechanical Engineering Education, School of Mechanical Engineering, Shandong University, Jinan, 250061, China.

High-performance osmotic energy conversion requires both large ionic throughput and high ionic selectivity, which can be significantly promoted by exterior surface charges simultaneously, especially for short nanopores. Here, we investigate the enhancement of ionic diffusion by charged exterior surfaces under various conditions and explore corresponding effective charged areas. From simulations, ionic diffusion is promoted more significantly by exterior surface charges through nanopores with a shorter length, wider diameter, and larger surface charge density or under higher salt gradients. Effective widths of the charged ring regions near nanopores are reversely proportional to the pore length and linearly dependent on the pore diameter, salt gradient, and surface charge density. Due to the important role of effective charged areas in the propagation of ionic diffusion through single nanopores to cases with porous membranes, our results may provide useful guidance to the design and fabrication of porous membranes for practical high-performance osmotic energy harvesting.
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http://dx.doi.org/10.1021/acs.jpclett.2c01351DOI Listing
June 2022

Involvement of NLRP3-inflammasome pathway in noise-induced hearing loss.

Neural Regen Res 2022 Dec;17(12):2750-2754

College of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital; National Clinical Research Center for Otolaryngologic Diseases; Key Laboratory of Hearing Science, Ministry of Education; Beijing Key Laboratory of Hearing Impairment for Prevention and Treatment, Beijing, China.

The inflammasome is a multiprotein oligomer in the cell cytoplasm and is part of the innate immune system. It plays a crucial role in the pathological process of noise-induced hearing loss (NIHL). However, the mechanisms of NLR family pyrin domain containing 3 (NLRP3) inflammasome activation in NIHL have not been clearly demonstrated. In this study, miniature pigs were exposed to white noise at 120 dB(A) and auditory brainstem response measurements were used to measure their hearing function. Immunofluorescence staining, confocal laser scanning microscopy, western blot assay, and quantitative reverse transcription-polymerase chain reaction were used to analyze inflammasome-related protein distribution and expression. NLRP3, interleukin-1β, interleukin-18, and cleaved-caspase-1 were highly expressed in the cochlea after 120 dB(A) white noise exposure. Our findings suggest that NLRP3-inflammasomes in the cochlea may be activated after acoustic trauma, which may be an important mechanism of noise-induced hearing loss.
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http://dx.doi.org/10.4103/1673-5374.339499DOI Listing
December 2022

A brief history of testicular organoids: from theory to the wards.

J Assist Reprod Genet 2022 Jun 2. Epub 2022 Jun 2.

Reproductive Medicine Center, The First Affiliated Hospital of China Naval Military Medical University, Shanghai, People's Republic of China.

In recent years, researchers have successfully generated many human and mammalian organoid models, including organoids representing the intestine, prostate, ovary, bladder, liver, and brain. Therefore, organoids have become an important research model in the fields of regenerative medicine, drug research, and gene therapy, acting as a bridge between in vivo and in vitro experiments. In addition, testicular organoids (TOs) represent the highest level of in vitro culture of spermatogenic cells in a simulated testicular environment. However, the generation of TOs is still in the early stages, and there is still much room for improvement in dealing with the many challenges and prospects to achieve the goal of TOs that simulate the testicular microenvironment in vitro or even reconstruct the process of spermatogenesis without the need to reconstruct seminiferous tubules. We review the brief history of TO generation and some major strategies for functional applications of TOs, which are the main concerns of our clinicians and laboratory researchers.
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http://dx.doi.org/10.1007/s10815-022-02529-6DOI Listing
June 2022

Comment on "Outcomes of different minimally invasive surgical treatments for vertebral compression fractures: An observational study".

World J Clin Cases 2022 Apr;10(12):3966-3968

Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China.

Recently we read the article entitled "Outcomes of different minimally invasive surgical treatments for vertebral compression fractures: An observational study". This was an observational study that reviewed the safety and efficacy of different cement augmentation modalities for vertebral compression fractures under osteoporotic condition. Overall, this is a valuable study that can provide a reference for clinical practice. On the other hand, we also noticed some points in the article and are willing to share our views. Further studies with a higher level of evidence can add more knowledge regarding relevant concerns.
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http://dx.doi.org/10.12998/wjcc.v10.i12.3966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100713PMC
April 2022

Real-World Evidence of Axicabtagene Ciloleucel for the Treatment of Large B Cell Lymphoma in the United States.

Transplant Cell Ther 2022 May 21. Epub 2022 May 21.

Medical College of Wisconsin/Center for International Blood and Marrow Transplant Research, Milwaukee, Wisconsin. Electronic address:

Axicabtagene ciloleucel (axi-cel) is a standard-of-care for patients with relapsed or refractory (r/r) large B cell lymphoma who have received 2 or more lines of prior therapy. Patients receiving axi-cel in the real world could have broader a demographic, disease, and treatment profile compared with that of the cohort in the pivotal ZUMA-1 trial. The present study was conducted to evaluate the outcomes of axi-cel therapy in the real-world setting. A total of 1297 patients receiving commercial axi-cel between 2017 and 2020 were selected from the Center for International Blood and Marrow Transplant Research's data registry, of whom 739 (57%) would have been ineligible for inclusion in the ZUMA-1 cohort. Efficacy and safety outcomes were described for the entire cohort and by ZUMA-1 eligibility. Their associations with age, Eastern Cooperative Oncology Group Performance Score, and comorbidities were evaluated using multivariable logistic and Cox regressions. At a median follow-up of 12.9 months, the overall response rate (ORR) was 73%, with a 56% complete response (CR) rate. Median overall survival (OS) and progression-free survival (PFS) were 21.8 months (95% confidence interval [CI], 17.4 to 28.8 months) and 8.6 months (95% CI, 6.5 to 12.1 months), respectively. Duration of response (DOR) was comparable in the ZUMA-1 ineligible patients and ZUMA-1 eligible patients (62% by 1 year [95% CI, 57% to 66%] versus 67% [95% CI, 62% to 72%]). Patients age ≥65 years had favorable ORR (odds ratio [OR], 1.39; 95% CI, 1.05 to 1.83) despite having a higher risk of cytokine release syndrome (CRS) (OR, 1.41; 95% CI, 1.02 to 1.94) and immune effector cell-associated neurotoxicity syndrome (ICANS) (OR, 1.77; 95% CI, 1.39-2.26). Eastern Cooperative Oncology Group Performance Score ≥2 was associated with inferior efficacy outcomes (OR for ORR, 0.32; 95% CI, 0.18-0.56; hazard ratio [HR] for OS, 3.27; 95% CI, 2.37 to 4.52) and higher incidence of ICANS (OR, 2.63; 95% CI, 1.40 to 4.93). The patients ineligible for ZUMA-1 still had a durable response with axi-cel. Elderly patients had favorable efficacy outcomes despite higher rates of CRS and ICANS. Patient selection for standard-of-care axi-cel should consider comorbidities and risk-to-benefit ratio rather than be based strictly on ZUMA-1 eligibility.
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http://dx.doi.org/10.1016/j.jtct.2022.05.026DOI Listing
May 2022

Spatiotemporal hysteresis distribution and decomposition of solar activities and climatic oscillation during 1900-2020.

Environ Res 2022 Sep 14;212(Pt C):113435. Epub 2022 May 14.

Department of Biological and Agricultural Engineering & Zachry Department of Civil Engineering, Texas A& M University, College Station, TX, 77843, USA.

Solar radiation is the external driving force of the Earth's climate system. In different spatial and temporal scales, meteorological elements have different responses and lag periods to solar activity (SA), climatic oscillation (CO), geographic factors (GF) and other influencing factors. However, such studies are not abundant and in-depth in the world. To further understand the "solar-climate-water resource" system, this study considers China as the study area and investigates the monthly data of temperature (T) and precipitation (P) during 1900-2020 that were obtained from 3836 grid stations. The strong interaction and lag distribution between T or P with SA and CO were studied and influence weights of SA, CO, and geographical factors (GF) of each grid station were calculated. A multivariate hysteretic decomposition model was established to simulate and quantitatively decompose the periodic lag considering the factors of the earth's revolution. It is found that the strong interaction/lag periods obtained in a long-time scale can be decomposed into several periods shorter than the SA period. The distribution of strong interaction/lag periods is nested with topography and echoes with cities. The underlying surface conditions and urbanization are also important factors affecting the T and P lag. There are two distinct dividing lines in the lag period and influencing factor pattern of T and P. The T dividing line moves through valleys where water or mountain ranges meet, where the gap facilitates monsoon movement across regions, while the P dividing line is a zone of dramatic terrain, where tall mountains block water vapor transport. In the lag trend of T, the northern region of China has the longest lag period, and the lag period of surrounding regions tends to converge to the northern region. The lag period caused by SN in southwest China is larger than that in northwest China, while the lag effect of CO is opposite in the above two regions. The lag trend of P also has the above characteristics, but the difference is that the lag period in central China is the longest.
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http://dx.doi.org/10.1016/j.envres.2022.113435DOI Listing
September 2022

Differential modulation of C. elegans motor behavior by NALCN and two-pore domain potassium channels.

PLoS Genet 2022 04 28;18(4):e1010126. Epub 2022 Apr 28.

Center for Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan, China.

Two-pore domain potassium channels (K2P) are a large family of "background" channels that allow outward "leak" of potassium ions. The NALCN/UNC80/UNC79 complex is a non-selective channel that allows inward flow of sodium and other cations. It is unclear how K2Ps and NALCN differentially modulate animal behavior. Here, we found that loss of function (lf) in the K2P gene twk-40 suppressed the reduced body curvatures of C. elegans NALCN(lf) mutants. twk-40(lf) caused a deep body curvature and extended backward locomotion, and these phenotypes appeared to be associated with neuron-specific expression of twk-40 and distinct twk-40 transcript isoforms. To survey the functions of other less studied K2P channels, we examined loss-of-function mutants of 13 additional twk genes expressed in the motor circuit and detected defective body curvature and/or locomotion in mutants of twk-2, twk-17, twk-30, twk-48, unc-58, and the previously reported twk-7. We generated presumptive gain-of-function (gf) mutations in twk-40, twk-2, twk-7, and unc-58 and found that they caused paralysis. Further analyses detected variable genetic interactions between twk-40 and other twk genes, an interdependence between twk-40 and twk-2, and opposite behavioral effects between NALCN and twk-2, twk-7, or unc-58. Finally, we found that the hydrophobicity/hydrophilicity property of TWK-40 residue 159 could affect the channel activity. Together, our study identified twk-40 as a novel modulator of the motor behavior, uncovered potential behavioral effects of five other K2P genes and suggests that NALCN and some K2Ps can oppositely affect C. elegans behavior.
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http://dx.doi.org/10.1371/journal.pgen.1010126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049526PMC
April 2022

Phase noise estimation based white light scanning interferometry for high-accuracy surface profiling.

Opt Express 2022 Mar;30(7):11912-11922

White light scanning interferometry (WLSI) has been an extremely powerful technique in precision measurements. In this work, a phase noise estimation based surface recovery algorithm is proposed, which can significantly improve the measurement accuracy by decreasing the noise level in phase map coming from the systemic and environmental disturbances. The noise existed in phase map is firstly researched in spectrum domain and defined as the linear combination of complex terms at each angular wavenumber. Afterwards, based on the theoretical linearity of the phase distribution, the surface features can be redefined through establishing the function with respect to phase noise. By applying least square estimation (LSE), a spectral coefficient is defined to determine the optimal estimation of phase noise that represents the best statistical consistency with the actual case, from which a more accurate surface after removing most phase noise will then be generated. In order to testify the noise elimination ability of the proposed method, a nano-scale step height standard (9.5nm±1.0nm) is scanned, and the measurement result 9.49nm with repeatability 0.17nm is successfully achieved. Moreover, a leading edge of an aero-engine blade is also tested to investigate the potential of this method in industrial inspections. The measurement comparison with AFM is also displayed.
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http://dx.doi.org/10.1364/OE.451746DOI Listing
March 2022

Binary Binder for Cf/C-SiC Composites with Enhanced Mechanical Property.

Materials (Basel) 2022 Apr 8;15(8). Epub 2022 Apr 8.

School of Materials Science and Engineering, Shandong University of Technology, Zibo 255000, China.

Cf/C-SiC composites have become the preferred material for high-temperature load-bearing applications because of their low density, high strength, and excellent thermal-physical properties. Due to the composite's poor sintering performance, the sintering temperature and pressure required for the preparation of Cf/C-SiC by traditional methods are also relatively high, which limits its engineering application. Herein, based on the precursor-derived ceramic route and C/C composites material preparation process, a binary binder (coal pitch and polysilylacetylene) is developed, which combines a carbon source, SiC precursor, and semi-ceramic SiC filler organically. Then, the SiC phase was successfully introduced into C/C composites by the slurry impregnation-hot pressing sintering method. The prepared Cf/C-SiC composites showed good mechanical properties, with a density of 1.53 g/cm and a bending strength of 339 ± 21 MPa. Moreover, the effects of the binary binder on the microstructure, density, and mechanical properties of Cf/C-SiC composites were investigated. This work provides a novel and effective approach to fabricating Cf/C-SiC composites with low density and high strength.
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http://dx.doi.org/10.3390/ma15082757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030504PMC
April 2022

Visual Analysis of Colorectal Cancer Immunotherapy: A Bibliometric Analysis From 2012 to 2021.

Front Immunol 2022 31;13:843106. Epub 2022 Mar 31.

The Second Clinical Medical College of Lanzhou University, Department of General Surgery, Lanzhou University Second Hospital, Key Laboratory of Digestive System Tumors of Gansu Province, Lanzhou, China.

An increasing number of studies have shown that immunotherapy serves a significant role in treating colorectal cancer (CRC) and has become a hotspot. However, few studies used the bibliometric method to analyze this field comprehensively. This study collected 1,899 records of CRC immunotherapy from 2012 to October 31, 2021, and used CiteSpace to analyze regions, institutions, journals, authors, and keywords to predict the latest trends in CRC immunotherapy research. The United States and China, contributing more than 60% of publications, were the main drivers in this field. Sun Yat-sen University was the most active institution, while the National Cancer Institute had the highest frequency of citations. Most publications were published in the Adam E Snook was the most prolific writer, while Dung T. Le was the most commonly co-cited author. "T cell", "MMI" and "PD-1blocked" were the most widely studied aspects of CRC immunotherapy. "Immune checkpoint inhibitor", "combination therapy", "drug therapy" and "liver metastases" were current research hotspots. "Tumor microenvironment", "neutrophils", "tumor-associated macrophages", and "suppressor cell" have emerged as research hotspots in recent years. "Gut microbiota", "nanoparticle" and "tumor mutational burden" as recently emerged frontiers of research that should be closely monitored.
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http://dx.doi.org/10.3389/fimmu.2022.843106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009266PMC
April 2022

Correction to: Sox10 Gene Is Required for the Survival of Saccular and Utricular Hair Cells in a Porcine Model.

Mol Neurobiol 2022 Jun;59(6):3336

College of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, 100853, China.

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http://dx.doi.org/10.1007/s12035-022-02812-8DOI Listing
June 2022

Infarction Patterns and Recurrent Adverse Cerebrovascular Events in Moyamoya Disease.

Dis Markers 2022 29;2022:8255018. Epub 2022 Mar 29.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

For moyamoya disease (MMD) patients who suffered an acute ischemic attack, the infarction patterns on DWI and its association with recurrent adverse cerebrovascular events (ACEs) after bypass surgery remain unknown. 327 patients who suffered an acute ischemic attack and received following revascularization surgery were retrospectively reviewed and were divided into three patterns according to the lesion number and distribution on DWI that obtained within 7 days of onset: no acute infarction (NAI), single acute infarction (SAI), and multiple acute infarctions (MAIs). We used Cox proportional hazard models to estimate hazard ratios (HR) for associations of infarction patterns and the risk of recurrent ACEs and strokes. Over a median follow-up of 41 months (IQR 26-60), there were 61 ACEs and 27 strokes. Compared to the NAI cohort, patients with SAI (HR, 2.92; 95% CI, 1.41-6.05; = 0.004) and MAIs (HR, 4.44; 95% CI, 2.10-9.41; < 0.001) were associated with higher risk of ACEs recurrences. In analysis adjusted for age and surgery modalities, the corresponding HR was 2.90 (95% CI: 1.41-5.98) for SAI and 4.10 (95% CI: 1.95-8.63) for MAIs, and this effect remained persistent on further adjustment for several potential confounders. Similar but less precise association was found in separate analysis that only takes into account stroke recurrences. Thus, different infarction patterns on DWI imply different risks of recurrent ACEs, and more attention should be paid to prevent ACEs in MMD patients with MAIs.
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http://dx.doi.org/10.1155/2022/8255018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8983186PMC
April 2022

Mapping the vocal circuitry of Alston's singing mouse with pseudorabies virus.

J Comp Neurol 2022 Aug 6;530(12):2075-2099. Epub 2022 Apr 6.

Department of Integrative Biology, The University of Texas at Austin, Austin, Texas, USA.

Vocalizations are often elaborate, rhythmically structured behaviors. Vocal motor patterns require close coordination of neural circuits governing the muscles of the larynx, jaw, and respiratory system. In the elaborate vocalization of Alston's singing mouse (Scotinomys teguina) each note of its rapid, frequency-modulated trill is accompanied by equally rapid modulation of breath and gape. To elucidate the neural circuitry underlying this behavior, we introduced the polysynaptic retrograde neuronal tracer pseudorabies virus (PRV) into the cricothyroid and digastricus muscles, which control frequency modulation and jaw opening, respectively. Each virus singly labels ipsilateral motoneurons (nucleus ambiguus for cricothyroid, and motor trigeminal nucleus for digastricus). We find that the two isogenic viruses heavily and bilaterally colabel neurons in the gigantocellular reticular formation, a putative central pattern generator. The viruses also show strong colabeling in compartments of the midbrain including the ventrolateral periaqueductal gray and the parabrachial nucleus, two structures strongly implicated in vocalizations. In the forebrain, regions important to social cognition and energy balance both exhibit extensive colabeling. This includes the paraventricular and arcuate nuclei of the hypothalamus, the lateral hypothalamus, preoptic area, extended amygdala, central amygdala, and the bed nucleus of the stria terminalis. Finally, we find doubly labeled neurons in M1 motor cortex previously described as laryngeal, as well as in the prelimbic cortex, which indicate these cortical regions play a role in vocal production. The progress of both viruses is broadly consistent with vertebrate-general patterns of vocal circuitry, as well as with circuit models derived from primate literature.
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http://dx.doi.org/10.1002/cne.25321DOI Listing
August 2022

SERS-based CRISPR/Cas assay on microfluidic paper analytical devices for supersensitive detection of pathogenic bacteria in foods.

Biosens Bioelectron 2022 Jul 17;207:114167. Epub 2022 Mar 17.

State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, China. Electronic address:

Rapid and point-of-need (PON) detection of bacteria is crucial to directly provide rapid and reliable diagnostics information during on-site tests, allowing more room for taking proactive measures. By taking the multifaceted advantages of CRISPR/Cas12a and surface-enhanced Raman scattering (SERS), for the first time, we designed a recombinase polymerase amplification (RPA)-integrated microfluidic paper-based analytical device (μPAD), coined RPA-Cas12a-μPAD for supersensitive SERS detection. Single-stranded DNAs were designed to "pull down" SERS nanoprobes. The amplicons of the invA gene triggered the trans-cleavage of Cas12a, resulting in the indiscriminate shredding of linker ssDNA. Thus, the degree of aggregation of SERS nanoprobes was dependent on the concentration of Salmonella typhimurium (S. typhi), which was determined on a μPAD and monitored by a Raman spectrometer. The limit of detection for S. typhi was approximately 3-4 CFU/mL for spiked milk and meat samples with a dynamic detection range from 1 to 10 CFU/mL. The RPA-Cas12a-μPAD secured accurate tests for food samples in 45 min. This work expands the reach of CRISPR-based diagnostics (CRISPR-Dx) and provides a novel and robust bacterial PON detection platform.
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http://dx.doi.org/10.1016/j.bios.2022.114167DOI Listing
July 2022

A Theoretical Framework for Human and Nonhuman Vocal Interaction.

Annu Rev Neurosci 2022 Feb 22. Epub 2022 Feb 22.

NYU Neuroscience Institute and Department of Otolaryngology, New York University Langone Medical Center, New York, NY, USA; email:

Vocal communication is a critical feature of social interaction across species; however, the relation between such behavior in humans and nonhumans remains unclear. To enable comparative investigation of this topic, we review the literature pertinent to interactive language use and identify the superset of cognitive operations involved in generating communicative action. We posit these functions comprise three intersecting multistep pathways: () the Content Pathway, which selects the movements constituting a response; () the Timing Pathway, which temporally structures responses; and () the Affect Pathway, which modulates response parameters according to internal state. These processing streams form the basis of the Convergent Pathways for Interaction framework, which provides a conceptual model for investigating the cognitive and neural computations underlying vocal communication across species. Expected final online publication date for the , Volume 45 is July 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.
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http://dx.doi.org/10.1146/annurev-neuro-111020-094807DOI Listing
February 2022

Identification of differentially expressed miRNAs derived from serum exosomes associated with gastric cancer by microarray analysis.

Clin Chim Acta 2022 Jun 14;531:25-35. Epub 2022 Mar 14.

The Second Clinical Medical College of Lanzhou University, Lanzhou, Gansu, China; Lanzhou University Second Hospital, Lanzhou, Gansu, China; Key Laboratory of Digestive System Tumors of Gansu Province, Lanzhou, Gansu, China. Electronic address:

Aims: To explore the differentially expressed microRNAs (DEMs) in serum exosomes between gastric cancer (GC) patients and healthy people to provide new targets for GC diagnosis and treatment.

Methods: DEMs in serum exosomes were screened by microarray analysis and verified by RT-qPCR. The target genes of DEMs were predicted using Targetscan and miRTarBase databases and then overlapped with the DEGs of STAD in TCGA database to obtain the common target genes. Biological function and pathway enrichment were analyzed using enrichr database, and a PPI network was constructed using STRING database. The potential target genes of DEMs were identified using the MCODE and cytoHubba plug-ins of Cytoscape software. Survival analysis were conducted using KMP and TCGA databases. The DEMs -target genes-pathways network was established using Cytoscape software. A Cox proportional hazards regression model formed by optimal target genes was used to access the reliability of this prediction process.

Results: Three serum exosomal microRNAs (exo-miRNAs, has-miR-1273 g-3p, has-miR-4793-3p, has-miR-619-5p) were identified to be highly expressed in GC patients and performed excellent diagnostic ability. A total of 179 common target genes related to GC were predicted. They were mainly involved in 79 GO functional annotations and 6 KEGG pathways. The prognostic model formed by eight optimal target genes (TIMELESS, DNA2, MELK, CHAF1B, DBF4, PAICS, CHEK1 and NCAPG2), which were low-risk genes of GC, also performed perfect prognostic ability.

Conclusions: Serum exosomal has-miR-1273 g-3p, has-miR-4793-3p and has-miR-619-5p can be used as new diagnostic biomarkers for GC. Among them, serum exosomal hsa-miR-1273 g-3p / hsa-miR-4793-3p targets MELK and hsa-miR-619-5p targets NCAPG2 were identified as novel mechanisms involved in the development of GC. It provides new targets for the diagnosis and treatment of GC by exo-miRNAs.
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http://dx.doi.org/10.1016/j.cca.2022.03.010DOI Listing
June 2022

Sox10 Gene Is Required for the Survival of Saccular and Utricular Hair Cells in a Porcine Model.

Mol Neurobiol 2022 Jun 5;59(6):3323-3335. Epub 2022 Mar 5.

College of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing, 100853, China.

Pathological changes of the cochlea and hearing loss have been well addressed in Waardenburg syndrome (WS). However, the vestibular organ malformation in WS is still largely unknown. In this study, the differentiation and development of vestibular sensory epithelium and vestibular function caused by SOX10 mutation, a critical gene induces WS, have been studied in minature pig model. Degeneration of vestibular hair cells was found in this Sox10 mutation porcine model. Inner ear phenotype of the SOX10 miniature pigs showed cochlear abnormalities as well as saccular hypofunction. In the mutant pigs, no prominent dissimilarity was shown in the bone structure of the semicircular canals. However, the saccular membrane was collapsed, and the infusion of stereocilia of the hair cells was observed. There were no dark cells in the utricles in the mutant pigs. The density of the utricular hair cells was also significantly lower in the mutant pigs compared to the wild type. Our study demonstrated that the SOX10 gene and melanocytes play important roles in the vestibular organ development. Sox10 mutation disrupts the KIT-DCT signaling pathway, affects the development of melanocytes, and leads to vestibule morphogenesis.
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http://dx.doi.org/10.1007/s12035-021-02691-5DOI Listing
June 2022

Sex-specific influence of communal breeding experience on parenting performance and fitness in a burying beetle.

R Soc Open Sci 2022 Feb 16;9(2):211179. Epub 2022 Feb 16.

Groningen Institute for Evolutionary Life Sciences (GELIFES), University of Groningen, 9712 CP Groningen, The Netherlands.

Communal breeding, wherein multiple conspecifics live and reproduce together, may generate short-term benefits in terms of defence and reproduction. However, its carry-over effects remain unclear. We experimentally tested the effects of communal breeding on parental care and reproduction in burying beetles (), which use carcasses as breeding resources and provide parental care to offspring. We subjected individuals to communal or non-communal breeding (i.e. pair breeding) during their first breeding event and to non-communal breeding during their second breeding event. We measured the parental care of individuals and of groups and the reproductive success of groups during both breeding events. In communal groups, large individuals became dominant and largely monopolized the carcass, whereas small individuals (i.e. subordinates) had restricted access to the carcass. At the first breeding event, large males in communal groups spent more time providing care than large males in non-communal groups, whereas such an effect was not observed for large females and small individuals. Reproductive successes were similar in communal and non-communal groups, indicating no short-term benefits of communal breeding in terms of reproduction. Compared with males from non-communal groups, males originating from communal groups produced a larger size of brood during their second breeding event, whereas such an effect was not observed for females. Our results demonstrate the sex-specific effects of communal breeding experience on parenting performance and fitness.
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http://dx.doi.org/10.1098/rsos.211179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8847889PMC
February 2022

Metformin reduces chondrocyte pyroptosis in an osteoarthritis mouse model by inhibiting NLRP3 inflammasome activation.

Exp Ther Med 2022 Mar 17;23(3):222. Epub 2022 Jan 17.

Orthopedics Ward 3, The General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China.

Osteoarthritis (OA) is an age-related degenerative disease, and its incidence is increasing with the ageing of the population. Metformin, as the first-line medication for the treatment of diabetes, has received increasing attention for its role in OA. The purpose of the present study was to confirm the therapeutic effect of metformin in a mouse model of OA and to determine the mechanism underlying the resultant delay in OA progression. The right knees of 8-week-old C57BL/6 male mice were subjected to destabilization of the medial meniscus (DMM). Metformin (200 mg/kg) was then administered daily for 4 or 8 weeks. Safranin O-fast green staining, H&E staining and micro-CT were used to analyse the structure and morphological changes. Immunohistochemical staining was used to detect type II collagen (Col II), matrix metalloproteinase 13 (MMP-13), NOD-like receptor protein 3 (NLRP3), caspase-1, gasdermin D (GSDMD) and IL-1β protein expression. Reverse transcription-quantitative PCR was used to detect the mRNA expression of NLRP3, caspase-1, GSDMD and IL-1β. Histomorphological staining showed that metformin delayed the progression of OA in the DMM model. With respect to cartilage, metformin decreased the Osteoarthritis Research Society International score, increased the thickness of hyaline cartilage and decreased the thickness of calcified cartilage. Regarding the mechanism, in cartilage, metformin increased the expression of Col II and decreased the expression of MMP-13, NLRP3, caspase-1, GSDMD and IL-1β. In addition, in subchondral bone, metformin inhibited osteophyte formation, increased the bone volume fraction (%) and the bone mineral density (g/cm), decreased the trabecular separation (mm) in early stage of osteoarthritis (4 weeks) but the opposite in an advanced stage of osteoarthritis (8 weeks). Overall, metformin inhibited the activation of NLRP3 inflammasome, decreased cartilage degradation, reversed subchondral bone remodelling and inhibited chondrocyte pyroptosis.
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http://dx.doi.org/10.3892/etm.2022.11146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8812147PMC
March 2022

An Array of Flag-Type Triboelectric Nanogenerators for Harvesting Wind Energy.

Nanomaterials (Basel) 2022 Feb 21;12(4). Epub 2022 Feb 21.

Maritime College, School of Electronics and Information Technology, Guangdong Ocean University, Zhanjiang 524088, China.

Harvesting wind energy from the ambient environment is a feasible method for powering wireless sensors and wireless transmission equipment. Triboelectric nanogenerators (TENGs) have proven to be a stable and promising technology for harvesting ambient wind energy. This study explores a new method for the performance enhancement and practical application of TENGs. An array of flag-type triboelectric nanogenerators (F-TENGs) for harvesting wind energy is proposed. An F-TENG consists of one piece of polytetrafluoroethylene (PTFE) membrane, which has two carbon-coated polyethylene terephthalate (PET) membranes on either side with their edges sealed. The PTFE was pre-ground to increase the initial charge on the surface and to enhance the effective contact area by improving the surface roughness, thus achieving a significant improvement in the output performance. The vertical and horizontal arrays of F-TENGs significantly improved the power output performance. The optimal power output performance was achieved when the vertical parallel distance was approximately 4/15 (see the main text for the meaning of ), and the horizontal parallel distance was approximately 2. We found that the peak output voltage and current of a single flag-type TENG of constant size were increased by 255% and 344%, respectively, reaching values of 64 V and 8 μA, respectively.
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http://dx.doi.org/10.3390/nano12040721DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8877856PMC
February 2022

Rapid fabrication of sub-micron scale functional optical microstructures on the optical fiber end faces by DMD-based lithography.

Opt Express 2022 Jan;30(1):676-688

The rapid development of optical fiber application systems puts forward higher requirements for the miniaturization and integration of optical fiber devices. One promising solution is to integrate diffractive optical microstructures on the end faces of optical fibers. However, rapid microfabrication on such tiny and irregular substrates is a challenge. In recent years, Femtosecond laser polymerization technology has become an effective solution to the challenge, which can be flexibly applied for the fabrication of complex 3D microstructures with ultra-high resolution. When the demand for the lithography resolution is not very high, other microfabrication methods with a lower technical threshold may be developed for achieving a balance between fabrication precision, cost and efficiency. In this paper, we report a Digital Micromirror Device (DMD) based lithography method dedicated to the fabrication of functional optical microstructures on the optical fiber end faces. Especially, it's also applicable to single-mode fibers (SMFs). By the projection via a 40x objective lens, the fabrication resolution of 0.405 μm was achieved within an exposure area of 209.92 μm × 157.44 μm. We evaluated the microfabrication results by the photomicrographs and the optical diffraction modulation effects of the functional optical microstructures. This method provides a new idea for fabricating both hybrid optical fiber devices and SMF devices, and it may be an alternative method for resolving the conflict between the precision, the cost and the efficiency.
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http://dx.doi.org/10.1364/OE.445677DOI Listing
January 2022

Responses of environmental and soil enzyme stoichiometric characteristics of wheat cropping system to fertilizer management in rain-fed areas of China.

Environ Sci Pollut Res Int 2022 Jun 30;29(27):41520-41533. Epub 2022 Jan 30.

College of Natural Resources and Environment, Northwest Agriculture & Forestry University, South Campus of Northwest A&F University, No. 3, Taicheng Road, Shaanxi, Yangling, Xianyang, China.

The application of organic fertilizer can improve soil fertility and maintain soil biodiversity. Soil enzyme activity can reflect the relationship between microbial nutrient demand and environmental nutrient availability. The experiment was established with a split-plot design, which included two main plots and two subplots. The main plots were 3 nitrogen levels (0, 150, 300 kg N ha). The two subplots were chemical fertilizer alone and combination of manure and inorganic application; the soil enzyme activity and chemical properties of each treatment were measured and analyzed. In the study, ecological enzyme stoichiometry was used to study the limitation of microbial resources in dryland wheat system. The results showed that the combined application of manure and chemical fertilizers did not significantly increase the activities of soil C, N and P cycling-related enzymes but decreased the activities of soil L-leucine aminopeptidase (LAP). Long-term application of organic fertilizer and mineral fertilizer significantly increased the accumulation of soil organic carbon (SOC) and nitrogen (TN) and increased soil microbial biomass (MBC, MBN). Organic fertilizer treatment significantly increased soil available phosphorus (AP) and soil NO-N contents, and decreased SOC/AP and TN/AP, but had no significant changes under different nitrogen application levels (N0, N1, N2). GHG emissions were increaseed with the amount of nitrogen applied, the addition of manure did not significantly increase the CO and NO emissions, and soil organic matter mineralization and gas emission fluxes decreased at ripen stage. The C-acquiring enzyme was negatively correlated with N-acquiring enzyme but positively correlated with P-acquiring enzyme. The microbial limiting effect of C and P on microbial metabolism becomes increasingly intense as the reproductive period progresses. Redundancy analysis of soil enzyme activities and chemical properties showed that soil TN and MBN could better explain the variation characteristics of soil enzyme activities. Therefore, the study of soil extracellular enzyme stoichiometry and microbial nutrient restriction can give us a more comprehensive understanding of the soil environment. There are more implications can be given under different nitrogen management modes and different growth stages. The results also provided an effective theoretical basis for regulating the changes of soil microbial environment.
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http://dx.doi.org/10.1007/s11356-021-18406-1DOI Listing
June 2022

Osteoclasts secrete leukemia inhibitory factor to promote abnormal bone remodeling of subchondral bone in osteoarthritis.

BMC Musculoskelet Disord 2022 Jan 25;23(1):87. Epub 2022 Jan 25.

Department of Orthopedics, General Hospital of Ningxia Medical University, 804 Shengli Street, Yinchuan, Ningxia, China.

Background: Osteoarthritis (OA) is a common chronic degenerative joint disease. At present, there is no effective treatment to check the progression of osteoarthritis. Osteochondral units are considered to be one of the most important structures affecting the occurrence and development of osteoarthritis. Osteoclasts mediate an increase in abnormal bone remodeling in subchondral bone in the early stage of osteoarthritis. Here, alendronate (ALN) that inhibit osteoclasts was used to study the regulatory effect of osteoclast-derived leukemia inhibitory factor (LIF) on early abnormal bone remodeling.

Methods: This study involved 10-week-old wild-type female C57BL/6 mice and female SOST knockout (KO) mice that were divided into the sham, vehicle, ALN, and SOST KO groups.

Results: The expression of LIF was found to decrease by inhibiting osteoclasts, and the histological OA score suggested that the degeneration of articular cartilage was attenuated. Additionally, micro-CT showed that osteoclasts inhibited in the early stage of OA could maintain the microstructure of the subchondral bone. The parameters of bone volume fraction (BV/TV), subchondral bone plate thickness (SBP.Th), and trabecular separation (Tb.Sp) of the treated group were better than those of the vehicle group.

Conclusions: These results suggested that downregulating the expression of sclerostin in osteocytes by secreting LIF from osteoclasts, activate the Wnt/β-catenin signaling pathway, and promote abnormal bone remodeling in OA. Therefore, clastokine LIF might be a potential molecular target to promote abnormal bone remodeling in early OA.
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http://dx.doi.org/10.1186/s12891-021-04886-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8790929PMC
January 2022

FAPI PET/CT in the Diagnosis of Abdominal and Pelvic Tumors.

Front Oncol 2021 4;11:797960. Epub 2022 Jan 4.

Department of Nuclear Medicine, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, China.

Positron emission tomography/computed tomography (PET/CT) with F-fluorodeoxyglucose (F-FDG) is currently a standard imaging examination used in clinical practice, and plays an essential role in preoperative systemic evaluation and tumor staging in patients with tumors. However, F-FDG PET/CT has certain limitations in imaging of some tumors, like gastric mucus adenocarcinoma, highly differentiated hepatocellular carcinoma, renal cell carcinoma, and peritoneal metastasis. Therefore, to search for new tumor diagnosis methods has always been an important topic in radiographic imaging research. Fibroblast activation protein (FAP) is highly expressed in many epithelial carcinomas, and various isotope-labelled fibroblast activation protein inhibitors (FAPI) show lower uptake in the brain and abdominal tissues than in tumor, thus achieving high image contrast and good tumor delineation. In addition to primary tumors, FAPI PET/CT is better than FDG PET/CT for detecting lymph nodes and metastases. Additionally, the highly selective tumor uptake of FAPI may open up new application areas for the non-invasive characterization, staging of tumors, as well as monitoring tumor treatment efficacy. This review focuses on the recent research progress of FAPI PET/CT in the application to abdominal and pelvic tumors, with the aim of providing new insights for diagnostic strategies for tumor patients, especially those with metastases.
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http://dx.doi.org/10.3389/fonc.2021.797960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8763785PMC
January 2022

The top-100 most cited articles of biomarkers in congenital heart disease: a bibliometric analysis.

Ann Palliat Med 2022 May 28;11(5):1700-1713. Epub 2021 Dec 28.

Evidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China; Gansu International Scientific and Technological Cooperation Base of Diagnosis and Treatment of Congenital Heart Disease, Lanzhou, China; Key Laboratory of Evidence Based Medicine and Knowledge Translation of Gansu Province, Lanzhou University, Lanzhou, China.

Background: Biomarkers have played an important role in the treatment and management of patients with congenital heart disease (CHD). The 100 most frequently cited articles addressing the possible role of biomarkers assessment in treatment and outcomes in patients with CHD were reviewed.

Methods: The Web of Science Core Collection database was selected as the database for this selection of publications. CiteSpace 5.7.R1 and VOSviewer 1.6.9 were used to analyze the information.

Results: A total of 877 articles referencing cardiac biomarkers and CHD were identified in the search period January 1980-June 2020. After screening, the top 100 most cited articles were finally determined. These articles were published in 56 journals, of which the Pediatric Cardiology published the most articles (n=8). Countries collaboration involved a total of 10 countries, and the visualized map indicated the USA had the strongest collaboration network. Related topics of future research will still focus on prevention, general condition evaluation, surgical prognosis evaluation, and application of natriuretic peptide in CHD.

Conclusions: We conducted an insight to acquainting characteristics of highly cited publications of biomarkers in CHD and highlighting the research subjects, global research trends, and network collaboration between countries. Related topics of frontiers will focus on: (I) the application of natriuretic peptide, (II) the diagnostic and prognostic value of genes and their related transcriptional translation agents, (III) the use of biomarkers to evaluate and predict the postoperative injury caused by extracorporeal circulation, (IV) and the application of other biomarkers (such as oxidative stress, homocysteine, and thrombosis) to assess and predict damage circumstance.
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http://dx.doi.org/10.21037/apm-21-2422DOI Listing
May 2022

Task-Oriented Convex Bilevel Optimization With Latent Feasibility.

IEEE Trans Image Process 2022 19;31:1190-1203. Epub 2022 Jan 19.

This paper firstly proposes a convex bilevel optimization paradigm to formulate and optimize popular learning and vision problems in real-world scenarios. Different from conventional approaches, which directly design their iteration schemes based on given problem formulation, we introduce a task-oriented energy as our latent constraint which integrates richer task information. By explicitly re- characterizing the feasibility, we establish an efficient and flexible algorithmic framework to tackle convex models with both shrunken solution space and powerful auxiliary (based on domain knowledge and data distribution of the task). In theory, we present the convergence analysis of our latent feasibility re- characterization based numerical strategy. We also analyze the stability of the theoretical convergence under computational error perturbation. Extensive numerical experiments are conducted to verify our theoretical findings and evaluate the practical performance of our method on different applications.
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http://dx.doi.org/10.1109/TIP.2022.3140607DOI Listing
January 2022

A speech planning network for interactive language use.

Nature 2022 02 5;602(7895):117-122. Epub 2022 Jan 5.

NYU Neuroscience Institute and Department of Otolaryngology, New York University Langone Medical Center, New York, NY, USA.

During conversation, people take turns speaking by rapidly responding to their partners while simultaneously avoiding interruption. Such interactions display a remarkable degree of coordination, as gaps between turns are typically about 200 milliseconds-approximately the duration of an eyeblink. These latencies are considerably shorter than those observed in simple word-production tasks, which indicates that speakers often plan their responses while listening to their partners. Although a distributed network of brain regions has been implicated in speech planning, the neural dynamics underlying the specific preparatory processes that enable rapid turn-taking are poorly understood. Here we use intracranial electrocorticography to precisely measure neural activity as participants perform interactive tasks, and we observe a functionally and anatomically distinct class of planning-related cortical dynamics. We localize these responses to a frontotemporal circuit centred on the language-critical caudal inferior frontal cortex (Broca's region) and the caudal middle frontal gyrus-a region not normally implicated in speech planning. Using a series of motor tasks, we then show that this planning network is more active when preparing speech as opposed to non-linguistic actions. Finally, we delineate planning-related circuitry during natural conversation that is nearly identical to the network mapped with our interactive tasks, and we find this circuit to be most active before participant speech during unconstrained turn-taking. Therefore, we have identified a speech planning network that is central to natural language generation during social interaction.
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http://dx.doi.org/10.1038/s41586-021-04270-zDOI Listing
February 2022
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