Publications by authors named "Liyuan Sun"

51 Publications

Neuroprotective effect of magnesium supplementation on cerebral ischemic diseases.

Life Sci 2021 May 22;272:119257. Epub 2021 Feb 22.

College of Biotechnology, Guilin Medical University, Guilin, Guangxi 541100, China. Electronic address:

Ischemic encephalopathy is associated with a high mortality and rate of disability. The most common type of ischemic encephalopathy, ischemic stroke, is the second leading cause of death in the world. At present, the main treatment for ischemic stroke is to reopen blocked blood vessels. However, despite revascularization, many patients are not able to achieve good functional results. At the same time, the strict time window (<4.5 h) of thrombolytic therapy limits clinical application. Therefore, it is important to explore effective neuroprotective drugs for the treatment of ischemic stroke. Magnesium is a natural calcium antagonist, which exerts neuroprotective effects through various mechanisms. However, while most basic studies have shown that magnesium supplementation can help treat cerebral ischemia, intravenous magnesium supplementation in large clinical trials has failed to improve prognosis of ischemic patients. Therefore, we review the basic and clinical studies of magnesium supplementation for cerebral ischemia. According to the route of administration, treatment can be divided into intraperitoneal magnesium supplementation, intravenous magnesium supplementation, arterial magnesium supplementation and intracranial magnesium supplementation. We also summarized the potential influencing factors of magnesium ion intervention in cerebral ischemia injury. Finally, in combination with influencing factors derived from basic research, this article proposes three future research directions, including magnesium supplementation into the circulatory system combined with magnesium supplementation in the lateral ventricle, magnesium supplementation in the lateral ventricle combined with hypothermia therapy, and lateral ventricle magnesium supplementation combined with intracarotid magnesium supplementation combined with selective hypothermia.
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http://dx.doi.org/10.1016/j.lfs.2021.119257DOI Listing
May 2021

Identification and Analyzation of Differentially Expressed Transcription Factors in Endometriosis.

Front Mol Biosci 2020 7;7:614427. Epub 2021 Jan 7.

Department of Gynecology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.

Endometriosis is interpreted as the existence of endometrium outside the uterine cavity, such as ovaries, fallopian tubes and pelvic cavity. Dysmenorrhea, abnormal menstruation, infertility, and chronic pelvic pain are the primary symptoms of endometriosis. Although there are many theories about the origin of endometriosis, the exact factor of the disease has not been confirmed. Therefore, many other mechanisms are still worth exploring. The gene lists of the transcription factors (TFs) were selected from the intersections of three databases. The limma R package was used to analyze the differentially expressed genes (DEGs) of GSE6364 and GSE7305 and the DEGs intersected with the TFs to obtain the differentially expressed TFs (DETFs). Subsequently, one-way ANOVA and Student's -test were used to analyze the expression of DETFs in different phases of the endometrium and the endometrium of the infertile and fertile females with endometriosis, respectively. Enrichment analysis and PPI network were performed to reveal the molecular mechanisms of endometriosis. Finally, the plotROC R package was used to evaluate the sensitivity and specificity of hub TFs for the diagnosis of endometriosis. A total of 54 DETFs were screened out in endometriosis. The expression of up-regulated DETFs was gradually increased from the early secretory to the proliferative phase of the endometrium. Most up-regulated DETFs increased expression in the endometrium of infertile females. The pathways of DETFs were mainly enriched in stem cell differentiation, transcription activity, steroid hormone receptor activity and herpes simplex virus. Two hub TFs (RUNX2 and BATF) and two sub-networks were finally acquired from the PPI network. RUNX2 and BATF also had high diagnostic value in endometriosis. We discovered and analyzed 54 DETFs that were closely related to endometriosis, which would contribute to explore new mechanisms of endometriosis and search for new diagnostic markers and effective therapeutic targets.
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http://dx.doi.org/10.3389/fmolb.2020.614427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817971PMC
January 2021

Exploration of the effects of altitude change on bacteria and fungi in the rumen of yak (Bos grunniens).

Arch Microbiol 2021 Mar 17;203(2):835-846. Epub 2020 Oct 17.

Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming, People's Republic of China.

The yak (Bos grunniens) is a ruminant animal with strong regional adaptability. However, little is known about the adaptation of the rumen microbial community of yaks at different altitudes and the adaptation mechanism of the host and intestinal microorganisms to the habitat. We investigated the adaptability of the rumen microorganisms of yaks at high and low altitudes. We also compared and analyzed the abundance and diversity of core microorganisms and those that varied between different animals. The aim was to compare the rumen bacterial and fungal communities of grazing yak living at two elevations. Bacteroidetes, Firmicutes, Ascomycota, and Chytridiomycota were the dominant bacteria in the plateau and low-altitude regions. Significant differences between the dominant microorganisms in the rumen of yaks were evident in the two regions. The proportion of fiber-degrading bacteria was significantly different between yaks dwelling at high-altitude and low-altitude regions. The abundance of starch-degrading bacteria was not significantly different with altitude. Species clustering similarity analysis showed that the rumen microorganisms in the two areas were obviously isolated and clustered into branches. Functional prediction showed significant differences in rumen microbial methane metabolism, starch and sucrose metabolism, ion-coupled transporter and bacterial secretion system at different altitudes. Overall, the results of this study improved our understanding of the abundance and composition of microorganisms in the rumen of yak at different altitudes.
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http://dx.doi.org/10.1007/s00203-020-02072-xDOI Listing
March 2021

Host and altitude factors affect rumen bacteria in cattle.

Braz J Microbiol 2020 Dec 19;51(4):1573-1583. Epub 2020 Sep 19.

Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming, 650201, People's Republic of China.

There are many kinds of microorganisms in the gastrointestinal tract of mammals, some of which are closely related to the host. Rumen microorganisms are essential for normal physiological activities of their host by decomposing plant crude lignin and providing essential nutrients. The composition and diversity of this microbial population are influenced by the host, environment, and diet. Despite its importance, little is known about the effects of factors such as altitude variation on rumen microbial population abundance and diversity in different ruminants. Here, we described the changes in overall rumen bacteria in four groups of cattle, including the Zhongdian yellow cattle and Zhongdian yaks, grazing at high altitudes (3600 m); the Jiangcheng yellow cattle and Jiangcheng buffalo were kept at an altitude of 1100 m. We found that there was a significant difference in rumen bacterial abundance of the Zhongdian yellow cattle and Zhongdian yaks at high altitude and there was obvious homogeneity in rumen bacterial abundance and diversity in the Jiangcheng yellow cattle and Jiangcheng buffalo at low altitude. Therefore, our research concluded that under the same dietary environment, there were differences in the abundance and diversity of certain bacteria in the rumen of different breeds of cattle, indicating that host genetic factors and intestinal microorganisms related to altitudinal variation had a greater influence on rumen bacterial abundance in the cattle.
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http://dx.doi.org/10.1007/s42770-020-00380-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688796PMC
December 2020

RNA sequencing of plasma exosomes revealed novel functional long noncoding RNAs in hepatocellular carcinoma.

Cancer Sci 2020 Sep 5;111(9):3338-3349. Epub 2020 Jul 5.

School of Public Health, Guangxi Medical University, Nanning, China.

Exosomal long noncoding RNA (lncRNA) has been found to be associated with the development of cancers. However, the expression characteristics and the biological roles of exosomal lncRNAs in hepatocellular carcinoma (HCC) remain unknown. Here, by RNA sequencing, we found 9440 mRNAs and 8572 lncRNAs were differentially expressed (DE-) in plasma exosomes between HCC patients and healthy controls. Exosomal DE-lncRNAs displayed higher expression levels and tissue specificity, lower expression variability and splicing efficiency than DE-mRNAs. Six candidate DE-lncRNAs (fold change 6 or more, P ≤ .01) were high in HCC cells and cell exosomes. The knockdown of these candidate DE-lncRNAs significantly affected the migration, proliferation, and apoptosis in HCC cells. In particular, a novel DE-lncRNA, RP11-85G21.1 (lnc85), promoted HCC cellular proliferation and migration by targeted binding and regulating of miR-324-5p. More importantly, the level of serum lnc85 was highly expressed in both Alpha-fetoprotein (AFP)-positive and AFP-negative HCC patients and allowed distinguishing AFP-negative HCC from healthy control and liver cirrhosis (area under the receiver operating characteristic curve, 0.869; sensitivity, 80.0%; specificity, 76.5%) with high accuracy. Our finding offers a new insight into the association between the dysregulation of exosomal lncRNA and HCC, suggesting that lnc85 could be a potential biomarker of HCC.
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http://dx.doi.org/10.1111/cas.14516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469810PMC
September 2020

The Expression of GLAST and GLT1 in a Transient Cerebral Ischemia Mongolian Gerbil Model.

Neuropsychiatr Dis Treat 2020 23;16:789-800. Epub 2020 Mar 23.

Department of Guangxi Key Laboratory of Brain and Cognitive Neuroscience, Guilin Medical University, Guilin, Guangxi 541004, People's Republic of China.

Purpose: Excitatory amino acid transporters (EAATs) have an indispensable function in the reuptake of extracellular glutamate. To investigate the relationship and the expression of neuronal and astrocytic markers after brain ischemia, the temporal profile of glial EAATs in both peripheral and core regions of the cortex was examined.

Methods: Transient common carotid artery occlusion was used to induce unilateral transient forebrain ischemia of Mongolian gerbils, and post-ischemic brains (6 h to 2 w) were collected and prepared for immunohistochemical and Western blotting analysis of glutamine synthetase (GS), GLT-1, GLAST, S100β, and NeuN, and for Alizarin red staining of calcium deposits.

Results: The expression of GLAST and GLT-1 were significantly escalated at 6 h both in the core and periphery regions, while reduced from 12 h to 2 w in the core region post-ischemia. GS-positive cells increased at 6 h both in the core and periphery regions, while the density of Alizarin red-positive cells increased and peaked at 12 h in the ischemic cortex. The density of S100β-positive cells decreased in the ischemic core and increased in the periphery region. Immunofluorescence staining showed that S100β and TUNEL double-positive cells increased at 12 h in the core region.

Conclusion: The results of GLT-1 and GLAST expression in the cortex indicate that their up-regulation was time-dependent and occurred in the acute post-ischemia period, whereas their down-regulation was region-dependent and it is involved in the pathological progress of nerve cell and glial cell death, and has a series of cascade reactions.
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http://dx.doi.org/10.2147/NDT.S238455DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125407PMC
March 2020

Directed modification of a ruminal cellulase gene (CMC-1) from a metagenomic library isolated from Yunnan gayal (Bos frontalis).

Arch Microbiol 2020 Jul 14;202(5):1117-1126. Epub 2020 Feb 14.

Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming, 650201, People's Republic of China.

Gayal (Bos frontalis) of the Yunnan region is well adapted to harsh environmental conditions. Its diet consists predominantly of bamboo, reeds, and woody plants, suggesting that the rumen of this species contains many fiber-degrading bacteria and cellulases. The aim of this study was to identify and modify specific cellulases found in the gayal rumen. In the present study, a directed evolution strategy of error-prone PCR was employed to improve the activity or optimal temperature of a cellulase gene (CMC-1) isolated from gayal rumen. The CMC-1 gene was heterologously expressed in Escherichia coli (E. coli) BL21, and the recombinant CMC-1 protein hydrolyzed carboxyl methyl cellulose (CMC) with an optimal activity at pH 5.0 and 50 °C. A library of mutated ruminal CMC-1 genes was constructed and a mutant EP-15 gene was identified. Sequencing analysis revealed that EP-15 and CMC-1 belonged to the glycosyl hydrolase family 5 (GHF5) and had the highest homology to a cellulase (Accession No. WP_083429257.1) from Prevotellaceae bacterium, HUN156. There were similar predicted GH5 domains in EP-15 and CMC-1. The EP-15 gene was heterologously expressed and exhibited cellulase activity in E. coli BL21 at pH 5.0, but the optimum temperature for its activity was reduced from that of CMC-1 (50 °C) to 45 °C, which was closer to the physiological temperature of the rumen (40 °C). The cellulase activity of EP-15 was about two times higher than CMC-1 at 45 °C or PH 5.0, and also was more stable in response to temperature and pH changes compared to CMC-1. This study successfully isolated and modified a ruminal cellulase gene from metagenomics library of Yunnan gayal. Our findings may obtain a useful cellulase in future applications and present the first evidence of modified cellulases in the gayal rumen.
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http://dx.doi.org/10.1007/s00203-020-01812-3DOI Listing
July 2020

Development and validation of a rapid kit for authenticity of murine meat in meat products with a species-specific PCR assay.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2020 Apr 10;37(4):552-560. Epub 2020 Feb 10.

Deptartment of Molecular Diagnosis, Jilin Leining Scientific Service Co. Ltd for Detection of Drug and Food, Jilin, Jilin, China.

Adulteration of meat products with murine meat poses a huge threat to consumer health and leads to serious disruption in food markets. Species authentication of murine meat is still technically challenging. We, therefore, developed a species-specific PCR kit consisting of murine meat DNA extraction, PCR reaction and identifying systems. We designed novel universal primers targeting highly conserved region on mitochondrial cytochrome b gene ( b) from four murines (lab rats, lab mice, wild rat and wild mice), as well as specific primers for meat from four widely consumed animal species, cattle, sheep, duck and donkey. Simultaneously, pasmid inserted specific b fragment was cloned and used as the internal positve control in the kit. The kit parameters of specificity, sensitivity, stability and validity were determined using mimic counterfeiting meatball. The specificity of the DNA detection kit was 100% in authentication of the four fraudulent meats of cattle, sheep, duck and donkey mixed murine meat. The minimum detection limit of the sample DNA was 0.1 μg. The kit, which had freeze-thawed up to 20 times and stored for 1 year, also was powerful in detecting an amount of 0.1 mg in artificial counterfeited cattle, sheep, duck and donkey meat products. The murine-species DNA detection kit proposed in this study has proved to be a simple, accurate and effective assay, and can be applied to the identification of murine meat traces in common edible meat, to ensure the realisable implementation of meat product market supervision.
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http://dx.doi.org/10.1080/19440049.2020.1718218DOI Listing
April 2020

Treatment Of Magnesium-L-Threonate Elevates The Magnesium Level In The Cerebrospinal Fluid And Attenuates Motor Deficits And Dopamine Neuron Loss In A Mouse Model Of Parkinson's disease.

Neuropsychiatr Dis Treat 2019 11;15:3143-3153. Epub 2019 Nov 11.

Department of Guangxi Key Laboratory of Brain and Cognitive Neuroscience, Guilin Medical University, Guilin 541004, Guangxi, People's Republic of China.

Purpose: Epidemiology research has demonstrated that magnesium (Mg) deficiency is associated with a high incidence of Parkinson's disease (PD). It is known that the systemic administration of MgSO is not able to elevate the Mg concentration in cerebrospinal fluid (CSF). This study aims to verify the protective effect of magnesium-L-threonate (MgT) in 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) mouse model.

Methods: C57BL/6J mice were orally administered MgT or MgSO for 4 weeks, and received MPTP in the third week. After analysis of open-field and rotarod tests on the last day, tyrosine hydroxylase (TH) immunopositive cells and protein levels were quantified in the substantia nigra pars compacta (SNpc) and striatum. The expression of inducible nitric oxide synthase (iNOS) level was evaluated. Mg concentration in serum and CSF was measured after oral administration of MgSO or MgT in normal mice. Mg concentration in the CSF was increased in the mice treated with MgT but not MgSO.

Results: The total distance and mean speed in open-field tests, and the time spent on rotarod in the MgT group were increased, compared with MPTP group. The MgT treatment but not MgSO dose-dependently attenuated the loss of TH-positive neurons, and the reduction of the TH expression in the SNpc. The MgT treatment also inhibited the expression of iNOS as measured by immunohistochemistry and Western blots. Double-immunofluorescence staining of TH and iNOS showed iNOS-positive cells were collocalized for TH-positive cells.

Conclusion: The treatment with MgT is associated with an increase of Mg in the CSF. MgT, rather than MgSO4, can significantly attenuate MPTP-induced motor deficits and dopamine (DA) neuron loss.
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http://dx.doi.org/10.2147/NDT.S230688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6857673PMC
November 2019

Concentration-regulated photon upconversion and quenching in NaYF:Yb,Er nanocrystals: nonexponentiality revisited.

Nanoscale 2019 Oct;11(39):18150-18158

Department of Chemistry, Renmin University of China, Beijing 100872, China.

Concentration quenching of rare-earth doped upconversion nanoparticles severely limits the dopant concentration, and this greatly hinders their potential applications. Therefore, it is necessary to understand the roles of dopant concentration in photon population and luminescence quenching for materials designed with improved upconversion luminescence (UCL). Herein, the excited-state dynamics of well-accepted NaYF4:Yb3+,Er3+ nanocrystals were investigated as models based on the Kohlrausch-function. The use of the Kohlrausch-function successfully disentangled the rise and decay of dynamics data and well revealed the kinetics. Photon population and concentration quenching mechanisms depending on the sensitizer concentration are deeply revealed by the regular variations of the fitting parameters. The results indicated that high doping of sensitizers will accelerate the population of both green and red emitting energy levels, but cause significant concentration quenching in green emission and little quenching in red emission. Our work opened up new pathways of kinetics analysis, which is beneficial for further mechanism development, and established detailed photon population and concentration quenching models depending on the doping concentration of the sensitizer.
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http://dx.doi.org/10.1039/c9nr06521kDOI Listing
October 2019

Elevated SH3BP5 Correlates with Poor Outcome and Contributes to the Growth of Acute Myeloid Leukemia Cells.

Biomolecules 2019 09 19;9(9). Epub 2019 Sep 19.

School of Basic Medical Sciences, Binzhou Medical University, Yantai 264003, China.

Current strategies are not especially successful in the treatment of acute myeloid leukemia (AML). The identification and characterization of oncogenes crucial to the survival and growth of leukemia cells will provide potential targets for the exploitation of novel therapies. Herein, we report that the elevated expression of SH3 domain-binding protein 5 (SH3BP5) significantly correlates with poor outcomes of AML patients. To test whether SH3BP5 contributes to the growth and survival of AML cells, we use the shRNA-encoding lentivirus system to achieve the knockdown of SH3BP5 expression in human AML cell lines U937, THP-1, Kasumi-1, and MV4-11. Functionally, the knockdown of SH3BP5 expression markedly inhibits the cell viability and induced apoptosis of these leukemia cells. Mechanistically, western blot analysis indicates that the knockdown of SH3BP5 expression decreases the phosphorylation of JNK and BAD. Moreover, the JNK agonist anisomycin rescues the growth inhibition phenotype of SH3BP5 deficiency in THP-1 cells. Moreover, the expression of SH3BP5 positively correlates with CD25 and CD123 levels. Finally, our study highlights the crucial role of SH3BP5 in promoting the survival of AML cells, and its suppression may be a potential therapeutic strategy for treating human AML.
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http://dx.doi.org/10.3390/biom9090505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770748PMC
September 2019

Development of a PCR-based assay for detection of Chinese mink tissue in meat products based on the mitochondrial DNA cytochrome- gene.

Mitochondrial DNA B Resour 2019 Jul 26;4(2):2748-2750. Epub 2019 Jul 26.

Jilin Leining Scientific Service Co. Ltd for Detection of Drug and Food, Jilin, China.

Species authentication of meat product origins has become an important subject for ensuring the health of consumers. Based on the cytochrome gene, we developed a PCR-based assay kit for identification of Chinese mink tissues from 10 animal species in meat products, and to evaluate its quality indices including specificity, stability, sensitivity, and repeatability. Kits were made up of DNA extraction and PCR amplification systems based on species-specific, and universal primers. The reference meat mixtures and commercial samples were extracted by the kit and PCR technique was performed to identify the species of mink authenticity. The kit was effective after 20 repeated freeze-thaw cycles and it could be stored at -20 °C for 1 year. The sensitivity showed that a concentration as low as 0.1 ng/μL still can amplify the target band. The specificity test confirmed that the kit was 100% specific. The kit proved to be effective, stable, and reliable for extraction of efficient contents of the genomic DNA and routine analysis of Chinese mink source composition from meat products.
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http://dx.doi.org/10.1080/23802359.2018.1532828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687377PMC
July 2019

detection of potential prognostic circRNAs through a re-annotation strategy in ovarian cancer.

Oncol Lett 2019 Apr 6;17(4):3677-3686. Epub 2019 Feb 6.

Department of Gynecology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.

Ovarian cancer (OC) is the most common and lethal gynecologic malignancy. The pathophysiology of OC tumor development is complex and involves numerous biological pathways. Previous studies suggest that circular (circ)RNAs serve important roles in OC tumor pathology. In the present study, a re-annotation strategy was performed to evaluate the expression level of circRNAs based on a microarray dataset obtained from the Gene Expression Omnibus database. Univariate and multivariate Cox regression analyses were performed to evaluate the association between survival and expression of circRNAs in each OC cohort. An expression-based risk score model was constructed to extrapolate the prognostic efficacy of this signature. In the GSE9891 dataset, the 278 OC patients were randomly divided into training and validating groups. A six-circRNA signature was significantly associated with overall survival in the training and validating datasets. The risk score model was further validated in GSE63885 and GSE26193 datasets. The six-circRNA signature was also significantly associated with patient progression-free survival and disease-free survival. Further investigation revealed that the signature had higher area under the curve values than the existing clinical and other molecular signatures in predicting survival. In conclusion, the present study revealed that the six-circRNA signature may serve as a potential prognostic biomarker of OC.
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http://dx.doi.org/10.3892/ol.2019.10021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425388PMC
April 2019

Natural selection on TMPRSS6 associated with the blunted erythropoiesis and improved blood viscosity in Tibetan pigs.

Comp Biochem Physiol B Biochem Mol Biol 2019 Jul 15;233:11-22. Epub 2019 Mar 15.

Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China. Electronic address:

Tibetan pigs, indigenous to Tibetan plateau, are well adapted to hypoxia. So far, there have been not any definitively described genes and functional sites responsible for hypoxia adaptation for the Tibetan pig. The whole genome-wide association studies in human suggested that genetic variations in TMPRSS6 was associated with hemoglobin concentration (HGB) and red cell counts (RBC). Here we conducted resequencing of the nearly entire genomic region (40.1 kb) of the candidate gene TMPRSS6 in 40 domestic pigs and 40 wild boars along continuous altitudes and identified 708 SNPs, in addition to an indel (CGTG/----) in the intron 10. We conduct the CGTG indel in 838 domestic pigs, both the CGTG deletion frequency and the pairwise r linkage disequilibrium showed an increase with elevated altitudes, suggesting that TMPRSS6 has been under Darwinian positive selection. As the conserved core sequence of hypoxia-response elements (HREs), the deletion of CGTG in Tibetan pigs decreased the expression levels of TMPRSS6 mRNA and protein in the liver revealed by real-time quantitative PCR and western blot, respectively. We compared domestic pigs and Tibetan pigs living continuous altitudes, found that the blood-related traits with the increase of altitude, however, the HGB did not increase with the elevation in Tibetan pigs. Genotype association analysis results dissected a genetic effect on reducing HGB by 13.25 g/L in Gongbo'gyamda Tibetan pigs, decreasing mean corpuscular volume (MCV) by 4.79 fl in Diqing Tibetan pigs. In conclusion, the CGTG deletion of TMPRSS6 resulted in lower HGB and smaller MCV, which could reflect a blunting erythropoiesis and improving blood viscosity as well as erythrocyte deformability. It remains to be determined whether a blunting of erythropoiesis for TMPRSS6 or others genetic effects are the physiological adaptations among Tibetan pigs.
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http://dx.doi.org/10.1016/j.cbpb.2019.03.003DOI Listing
July 2019

Short-term effect of intravitreal conbercept injection on major and macular branch retinal vein occlusion.

J Int Med Res 2019 Mar 25;47(3):1202-1209. Epub 2019 Jan 25.

2 Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan, Shandong, P.R. China.

Objective: To evaluate the effectiveness of intravitreal conbercept injection on major and macular branch retinal vein occlusion (BRVO).

Methods: This retrospective analysis involved 43 patients with BRVO (major BRVO n = 24; macular BRVO, n = 19) who were diagnosed by fluorescein fundus angiography (FFA) and injected with intravitreal conbercept. The following outcomes were measured at baseline and follow-up (1-6 months): best-corrected visual acuity (BCVA), central foveal thickness (CFT), total retinal volume in a 6-mm diameter section of the macula, choroidal thickness under the central fovea of the macula, relative area of retinal hemorrhage, complications, and times when repeated injection was performed.

Results: There were significant differences between the two groups in terms of BCVA, CFT, and total retinal volume in a 6-mm diameter section of the macula at 6 months after treatment. Choroidal thickness under the central fovea of the macula and relative area of retinal hemorrhage showed no significant differences between the two groups at 6 months after treatment; however, they significantly differed from baseline measurements.

Conclusion: In general, intravitreal injection of conbercept may have a better short-term effect in patients with macular BRVO than in patients with major BRVO.
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http://dx.doi.org/10.1177/0300060518819613DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421366PMC
March 2019

[Development on DNA detection kit of fox and its application in common meat products].

Wei Sheng Yan Jiu 2018 Nov;47(6):979-983

School of Medical Technology, Beihua University, Jilin 132013, China.

Objective: To develop a kind of DNA extraction and detection kit for identification of fox source composition.

Methods: Using the modern DNA fingerprint technology, the DNA extraction method was improved, and DNA extraction and detection reagent was developed to obtain the fox polymerase chain reaction( PCR)detection kit. The performance parameters of the kit were evaluated. Finally, 42 samples of fox meat and its mixture with commercial meat products were detected.

Results: The kit was proved effective after 20 times of the repeated frozen-thaw and it could be stored at-20 ℃ for 1 year. The specificity test confirmed that fox source composition were detected from 42 samples of fox meat and its mixture with commercial meat. The specificity of the kit was 100%. The minimum detection limit of DNA was 0. 1 ng/μL.

Conclusion: The fox DNA detection kit could be applied in rapid detection commonmeat of fox source composition, which are good specificity, high sensitivity and good stability.
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November 2018

Identification of potential prognostic TF-associated lncRNAs for predicting survival in ovarian cancer.

J Cell Mol Med 2019 03 13;23(3):1840-1851. Epub 2018 Dec 13.

The First Affiliated Hospital, Harbin Medical University, Harbin, China.

The pathophysiology of ovarian cancer (OV) is complex and depends on multiple biological processes and pathways. Therefore, there is an urgent need to identify reliable prognostic biomarkers for predicting clinical outcomes and helping personalize treatment of OV. A long non-coding RNA (lncRNA)-based risk score model was constructed to infer the prognostic efficacy of transcription factors (TFs) based on the OV dataset from The Cancer Genome Atlas. The risk score model was further validated in other independent cohorts from Gene Expression Omnibus. Time-dependent receiver operating characteristic curves were used to evaluate the survival prediction performance in comparison with other clinical and molecular variables. Our results revealed that the top-ranked TF-associating lncRNAs were significantly associated with overall survival, progression-free survival and disease-free survival. Stratification analysis according to clinical variables indicated the prognostic independence of POLR2A-associating lncRNAs. In comparison, the signature of POLR2A-associating lncRNAs was more sensitive and specific than existing clinical and molecular signatures. Functional and experimental analysis suggested that POLR2A-associating lncRNAs may be involved in known biological processes and pathways of OV. Our findings revealed that the lncRNA-based risk score model can provide helpful information on OV prognosis stratification and discovery of therapeutic biomarkers.
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http://dx.doi.org/10.1111/jcmm.14084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378234PMC
March 2019

Psychosocial Predictors of Adjustment to Disability Among Patients With Breast Cancer: A Cross-Sectional Descriptive Study.

J Nurs Res 2019 Apr;27(2):e15

MMed, RN, Nurse, Department of Plastic and Reconstructive Surgery, Tianjin Medical University Cancer Institute & Hospital, China.

Background: Acceptance of disability relates to the process of adaptation, which reflects how the values of a patient change over time to accept herself or himself as an intrinsically worthy person. Acceptance of disability (AOD) plays an important role in the adjustment of persons with physical disability to their environment. However, no studies on the level of AOD in patients with breast cancer are available in the literature.

Purpose: The aim of this study was to explore the significant factors that influence disability acceptance in Chinese women with breast cancer.

Methods: A cross-sectional study was conducted on 292 patients with breast cancer between January and April 2017. Participants were assessed using the Acceptance of Disability Scale-Revised, Medical Coping Modes Questionnaire, Sense of Coherence Scale, Social Relational Quality Scale, and general information questionnaire. Multiple regression analysis was used to determine the factors associated with the level of disability acceptance.

Results: The mean score for AOD was 79.14 and ranged from 32 to 128. Multiple regression analysis indicated that family intimacy, friendships, confrontation, manageability, family commitment, marital status, surgery, and acceptance-resignation significantly affected disability acceptance, explaining 49.1% of the total variance in disability acceptance.

Conclusions/implications For Practice: The participants had a moderate level of disability acceptance. Disability acceptance is an important factor in the psychosocial adaptation to cancer. Healthcare staff should pay special attention to low scores for this factor and explore the potential of psychological interventions to effectively reduce the posttraumatic stress response, encourage the adoption of positive coping strategies, and hasten disability acceptance and return to society.
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http://dx.doi.org/10.1097/jnr.0000000000000283DOI Listing
April 2019

Coumarin-dithiocarbamate hybrids as novel multitarget AChE and MAO-B inhibitors against Alzheimer's disease: Design, synthesis and biological evaluation.

Bioorg Chem 2018 12 11;81:512-528. Epub 2018 Sep 11.

National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, PR China. Electronic address:

A series of new coumarin-dithiocarbamate hybrids were designed and synthesized as multitarget agents for the treatment of Alzheimer's disease. Most of them showed potent and clearly selective inhibition towards AChE and MAO-B. Among these compounds, compound 8f demonstrated the most potent inhibition to AChE with IC values of 0.0068 μM and 0.0089 μM for eeAChE and hAChE, respectively. Compound 8g was identified as the most potent inhibitor to hMAO-B, and it is also a good and balanced inhibitor to both hAChE and hMAO-B (0.114 µM for hAChE; 0.101 µM for hMAO-B). Kinetic and molecular modeling studies revealed that 8g was a dual binding site inhibitor for AChE and a competitive inhibitor for MAO-B. Further studies indicated that 8g could penetrate the BBB and exhibit no toxicity on SH-SY5Y neuroblastoma cells. More importantly, 8g did not display any acute toxicity in mice at doses up to 2500 mg/kg and could reverse the cognitive dysfunction of scopolamine-induced AD mice. Overall, these results highlighted 8g as a potential multitarget agent for AD treatment and offered a starting point for design of new multitarget AChE/MAO-B inhibitors based on dithiocarbamate scaffold.
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http://dx.doi.org/10.1016/j.bioorg.2018.09.010DOI Listing
December 2018

Abnormal activation of the sonic hedgehog signaling pathway in endometriosis and its diagnostic potency.

Fertil Steril 2018 07;110(1):128-136.e2

Department of Gynecology, the First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China. Electronic address:

Objective: To investigate the abnormal expression of sonic hedgehog (SHH) signaling molecules in 52 eutopic endometrial tissues and its diagnostic potency in endometriosis.

Design: Retrospective study.

Setting: University hospital.

Patient(s): Twenty-six women with histologically confirmed endometriosis and 26 women with histologically normal endometria who were undergoing curettage or hysterectomy were selected.

Intervention(s): None.

Main Outcome Measure(s): The mRNA and protein levels of molecules in the SHH signaling pathway.

Result(s): The levels of SHH, smoothened, GLI family zinc finger 3, and its downstream signaling transcription factor (GLI1) not only were upregulated in the eutopic endometrium of endometriosis compared with the control endometrium, but also independently predicted the onset and severity of the disease.

Conclusion(s): This study is the first to reveal differences in the activation of the SHH signaling pathway between women with and without endometriosis and suggests that the SHH signaling pathway has potential in the diagnosis of endometriosis.
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http://dx.doi.org/10.1016/j.fertnstert.2018.02.138DOI Listing
July 2018

Energy transfer mechanism dominated by the doping location of activators in rare-earth upconversion nanoparticles.

Phys Chem Chem Phys 2018 Jun;20(25):17141-17147

Department of Chemistry, Renmin University of China, Beijing 100872, China.

Research on the energy transfer mechanism of rare-earth-doped upconversion nanoparticles (UCNPs) has been an important area due to the increasing demand for tuning multicolor emission and enhancing the upconversion efficiency; however, because of large energy mismatch, many lanthanide activators, such as Eu3+, cannot realize highly efficient near infrared-to-visible upconversion by simple codoping of Yb3+. Therefore, introduction of other ions to assist the energy transfer process is required. Herein, we prepared core-shell nanoparticles with different doping locations to investigate the upconversion energy transfer mechanism. The upconversion luminescence (UCL) of core-shell nanoparticles was investigated by steady-state luminescence and time-resolved luminescence spectra. The UCL behaviors in these different multi-activator core-shell nanoparticles were observed. The results revealed different energy transfer channels influenced by the doping location of activators. This study may open up new avenues of structure design for fine-tuning of multicolor UCL for specific applications.
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http://dx.doi.org/10.1039/c8cp02142bDOI Listing
June 2018

Reactive oxygen species mediates 50-Hz magnetic field-induced EGF receptor clustering via acid sphingomyelinase activation.

Int J Radiat Biol 2018 07 30;94(7):678-684. Epub 2018 May 30.

a Bioelectromagnetics Key Laboratory, Zhejiang University School of Medicine , Hangzhou , China.

Purpose: Exposure to extremely low frequency electromagnetic fields (ELF-EMFs) could elicit biological effects including carcinogenesis. However, the detailed mechanisms by which these ELF-EMFs interact with biological system are currently unclear. Previously, we found that a 50-Hz magnetic field (MF) exposure could induce epidermal growth factor receptor (EGFR) clustering and phosphorylation on cell membranes. In the present experiment, the possible roles of reactive oxygen species (ROS) in MF-induced EGFR clustering were investigated.

Materials And Methods: Human amnion epithelial (FL) cells were exposed to a 50-Hz MF with or without N-acetyl-l-cysteine (NAC) or pyrrolidine dithiocarbamate (PDTC). EGFR clustering on cellular membrane surface was analyzed using confocal microscopy after indirect immunofluorescence staining. The intracellular ROS level and acid sphingomyelinase (ASMase) activity were detected using an ROS assay kit and an Amplex Red Sphingomyelinase Assay Kit, respectively.

Results: Results showed that exposure of FL cells to a 50-Hz MF at 0.4 mT for 15 min significantly enhanced the ROS level, induced EGFR clustering and increased ASMase activity. However, pretreatment with NAC or PDTC, the scavenger of ROS, not only counteracted the effects of a 50-Hz MF on ROS level and AMS activity, but also inhibited the EGFR clustering induced by MF exposure.

Conclusions: The present and previous data suggest that ROS mediates the MF-induced EGFR clustering via ASMase activation.
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http://dx.doi.org/10.1080/09553002.2018.1466208DOI Listing
July 2018

Chrysotoxene induces apoptosis of human hepatoblastoma HepG2 cells and via activation of the mitochondria-mediated apoptotic signaling pathway.

Oncol Lett 2018 Apr 25;15(4):4611-4618. Epub 2018 Jan 25.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chinese People's Liberation Army General Hospital, Beijing 100048, P.R. China.

Previous studies have indicated that chrysotoxene may be a potential drug used to treat tumors, however the effect of chrysotoxene on hepatoblastoma remains unknown. Therefore, the present study aimed to investigate the cytotoxic effect and elucidate the potential molecular mechanism of chrysotoxene on human hepatoblastoma HepG2 cells. Chrysotoxene (5-40 µg/ml) exhibited cytotoxic activity against HepG2 cells with inhibitory rates of 24.67-84.06% (half maximal inhibitory concentration, 19.64 µg/ml), observed from a Cell Counting Kit-8 assay. The results of flow cytometry analysis indicated that chrysotoxene (5, 10 or 20 µg/ml) significantly (P<0.01) induced the apoptosis of HepG2 cells with apoptotic rates of 23.14, 35.68 and 55.61% respectively, compared with the control group. The results of western blot analysis indicated that chrysotoxene (5, 10 or 20 µg/ml) significantly (P<0.05) promoted the release of second mitochondria-derived activator of caspase (Smac) and Cytochrome c from the mitochondria to the cytoplasm, downregulated Survivin and B cell lymphoma-2 (Bcl-2) proteins levels, and upregulated Bcl-2-associated X factor (Bax), apoptotic protease activating factor-1 (Apaf-1), cleaved (c)-caspase-9 and c-caspase-3 protein levels in HepG2 cells, compared with the control group. The results of xenograft analysis indicated that chrysotoxene (20 mg/kg) significantly (P<0.01) inhibited the growth of HepG2 cell-induced tumors by regulating the aforementioned apoptotic proteins (Smac, Cytochrome c, Survivin, Bcl-2, Bax, Apaf-1, c-caspase-9 and c-caspase-3), compared with the control group. In conclusion, chrysotoxene induced the apoptosis of HepG2 cells and via activation of the mitochondria-mediated apoptotic signaling pathway, suggesting that it may be a potential candidate drug for treating patients with hepatoblastoma.
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http://dx.doi.org/10.3892/ol.2018.7857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5835886PMC
April 2018

Synthesis of EU-1/ZSM-48 Co-Crystalline Zeolites from High-Silica EU-1 Seeds: Tailoring Phase Proportions and Promoting Long Crystalline-Phase Stability.

Chemistry 2018 May 17;24(25):6595-6605. Epub 2018 Apr 17.

State Key Laboratory of Heavy Oil Processing and the Key Laboratory of Catalysis of CNPC, China University of Petroleum-Beijing, 102249, Beijing, P. R. China.

A facile, specific, seed-assisted strategy for the synthesis of EU-1/ZSM-48 co-crystalline zeolites in the presence of hexamethonium ions (HM ) has been developed. EU-1/ZSM-48 co-crystalline zeolites with various phase proportions, with EU-1 in the range of 25 wt %-86 wt %, were obtained by adding high-silica EU-1 seeds (SiO /Al O ratio of 300) and adjusting the synthesis parameters. Not only can the phase proportions of EU-1/ZSM-48 co-crystalline zeolites be controlled, but also the stability period for co-crystallization of the two phases can be extended through varying the amount of EU-1 seeds and the HM template. Moreover, with the increase of the EU-1 proportion in the EU-1/ZSM-48 co-crystalline, the framework SiO /Al O ratios of EU-1 phase promotes steadily. Major differences in acidity and textural properties of the EU-1/ZSM-48 co-crystalline zeolites (Coz) were found with varying phase proportions, due to their distinct topological structures, crystal morphology and asymmetry between the EU-1 and ZSM-48 phases. For instance, the EU-1/ZSM-48 zeolite containing 75 wt % of EU-1 (Coz-75) possesses specific acidity and mesoporous characteristics, showing an excellent catalytic activity and stability in n-hexane cracking reaction. Compared to EU-1, ZSM-48, and a mechanical mix of the two zeolites (Mix-75), Coz-75 resulted in the highest hexane conversion and yields of light olefins, with a propylene yield, in particular, up to 38.3 wt %, which is 6.3 % more than that of the Mix-75 sample.
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http://dx.doi.org/10.1002/chem.201705805DOI Listing
May 2018

Long non-coding RNA CARLo-5 promotes tumor progression in hepatocellular carcinoma via suppressing miR-200b expression.

Oncotarget 2017 Sep 26;8(41):70172-70182. Epub 2017 Jul 26.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing, China.

Long non-coding RNAs (lncRNAs) play key roles in cancer initiation and progression. The aim was to investigate the biological functions and clinical significance of long non-coding RNA CARLo-5 in hepatocellular carcinoma (HCC). QRT-PCR was performed to investigate CARLo-5 expression in HCC tissues and cells. Kaplan-Meier curve and multivariate analysis validated the association between CARLo-5 expression and overall survival (OS) in HCC patients. Cell proliferation and invasion was performed by CCK8 cell proliferation, cell colony formation and transwell invasion assays. Western-blot assay was performed to evaluate the protein expression of Twist1, ZEB1, E-cadherin and Vimentin. Tumor xenografts were performed to evaluate the effect of CARLo-5 on tumor growth . RNA Immunoprecipitation (RIP) and Chromatin Immunoprecipitation (ChIP) were also performed. Our results showed that CARLo-5 expression was significantly higher in HCC tissues and upregulated CARLo-5 expression was closely correlated with tumor size and advanced tumor stage. Kaplan-Meier curve and multivariate analysis validated that higher CARLo-5 expression predicted a poor prognosis for HCC patients and was an independent risk factor for OS in HCC patients. , knockdown of CARLo-5 inhibited cell proliferation, colony formation, cell invasion and inhibited the cell epithelial-mesenchymal transition (EMT) by up-regulating the E-cadherin expression and down-regulating Twist1, ZEB1 and vimentin expression in HCC cells. Furthermore, we demonstrated that CARLo-5 inhibited the miR-200b expression via EZH2. , knockdown of CARLo-5 significantly inhibited the tumor growth. Thus, our results indicated that CARLo-5 represented a novel tumor biomarker and therapeutic target for HCC.
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http://dx.doi.org/10.18632/oncotarget.19597DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5642544PMC
September 2017

Characteristics of Human Endometrium-Derived Mesenchymal Stem Cells and Their Tropism to Endometriosis.

Stem Cells Int 2017 6;2017:4794827. Epub 2017 Jul 6.

Department of Gynaecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

Human endometrial tissue has become an attractive source of mesenchymal stem cells (MSCs) for cell-based therapies because these MSCs can be easily harvested and have tumour tropism as well as reduced immunogenic and inflammatory properties. Our study aimed to obtain and characterise human endometrial mesenchymal stem cells (EMSCs) and assess their endometriosis tropism. EMSCs were successfully isolated from the endometrium of women undergoing laparoscopy for idiopathic infertility. The EMSCs presented a fibroblast-like morphology during culture. Flow cytometry analyses showed that the cells were positive for the specific stem cell markers CD73, CD90, CD105, CD166, and HLA-ABC (major histocompatibility complex class I (MHC I)) but negative for CD14, CD34, CD45, and HLA-DR (MHC II). Reverse transcription polymerase chain reaction results showed that the EMSCs expressed the stem cell marker OCT4. The EMSCs could differentiate into osteocytes, adipocytes, and chondrocytes under certain conditions. The EMSCs had a high tropism to endometriosis without tumourigenicity. This study enhances the possibility of using EMSCs as drug carriers in human cell-based therapies. Meanwhile, future research could also focus on developing targeted therapies for endometriosis.
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http://dx.doi.org/10.1155/2017/4794827DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5518492PMC
July 2017

FOXF2 deficiency promotes hepatocellular carcinoma metastasis by inducing mesenchymal-epithelial transition.

Cancer Biomark 2017 Jul;19(4):447-454

Objective: The transcription factor FOXF2 is reported to be down-regulated in HCC. Its deficiency is correlated with shorter disease-free survival and overall survival of HCC patients; however, the mechanism remains to be elucidated.

Materials And Methods: In this study, we performed qRT-PCR and western blotting to confirm the down-regulated FOXF2 in HCC tissue and cell lines. Then the HCC cell line Huh7 transduced with FOXF2 shRNA was adopted in a series of in vitro and in vivo assays to evaluate the cell phenotype change, migration, invasion, proliferation, colonization of circulating cell and the formation of metastatic nodules.

Results: We found that FOXF2 was down-regulated in HCC tissues and cell lines. FOXF2 deficiency in Huh7 cells increased E-cadherin and decreased Vimentin. The down-regulation of FOXF2 impeded HCC cell migration and invasion capacity, but promoted the proliferation of HCC cells and the growth of subcutaneous tumors in nude mice, which indicated a mesenchymal-to-epithelial phenotypic change in Huh7 cells. FOXF2 deficiency enhanced the colonization of circulating HCC cell, thus promoted the formation of metastatic nodules.

Conclusions: FOXF2 deficiency induced mesenchymal-epithelial transition (MET) in Huh7 cell which might facilitate the colonization of circulating tumor cells and the formation of metastasis.
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http://dx.doi.org/10.3233/CBM-170139DOI Listing
July 2017

Long non-coding RNA colon cancer-associated transcript 1 functions as a competing endogenous RNA to regulate cyclin-dependent kinase 1 expression by sponging miR-490-3p in hepatocellular carcinoma progression.

Tumour Biol 2017 Apr;39(4):1010428317697572

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing, China.

Hepatocellular carcinoma is an aggressive neoplasm and is one of the most common human cancers. Recently, long non-coding RNAs have been demonstrated to participate in pathogenesis of many diseases including the progression in several cancers. In this study, we found that the long non-coding RNA colon cancer-associated transcript 1 was upregulated in hepatocellular carcinoma tissues (p < 0.05), and high colon cancer-associated transcript 1 expression level was positively associated with tumor volume (p < 0.05) and American Joint Committee on Cancer stage (p < 0.05) in hepatocellular carcinoma patients. Luciferase reporter assays and RNA-pulldown assays showed that colon cancer-associated transcript 1 is a target of miR-490-3p. Real-time quantitative polymerase chain reaction and Western blot analysis indicated that colon cancer-associated transcript 1 regulated cyclin-dependent kinase 1 expression as a competing endogenous RNA by sponging miR-490-3p in hepatocellular carcinoma cells. Furthermore, colon cancer-associated transcript 1 silencing decreased hepatocellular carcinoma cells proliferation and invasion and overexpression promoted cell proliferation and invasion in vitro. These data demonstrated that the colon cancer-associated transcript 1/miR-490-3p/cyclin-dependent kinase 1 regulatory pathway promotes the progression of hepatocellular carcinoma. Inhibition of colon cancer-associated transcript 1 expression may be a novel therapeutic strategy for hepatocellular carcinoma.
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http://dx.doi.org/10.1177/1010428317697572DOI Listing
April 2017

Induction of specific T helper-9 cells to inhibit glioma cell growth.

Oncotarget 2017 Jan;8(3):4864-4874

Department of Neurosurgery, Guilin Medical University, Affiliated Hospital, Guilin, 541001, China.

The effects of Staphylococcal enterotoxin B (SEB) on regulation of immune response have been recognized; whether SEB can enhance the effects of immunotherapy on glioma remains to be investigated. This study tests a hypothesis that administration with SEB enhances the effects of specific immunotherapy on glioma growth in mice. In this study, a glioma-bearing mouse model was developed by adoptive transfer with GL261 cells (a mouse glioma cell line). The mice were treated with the GL261 cell extracts (used as an Ag) with or without administration of SEB. We observed that treating glioma-bearing mice with the glioma Ag and SEB induced glioma-specific Th9 cells in both glioma tissue and the spleen. Treating CD4+ CD25- T cells with SEB increased p300 phosphorylation, histone H3K4 acetylation at the interleukin (IL)-9 promoter locus, and increased the IL-9 transcriptional factor binding to the IL-9 promoter. Treating CD4+ CD25- T cells with both SEB and glioma Ag induced glioma-specific Th9 cells. The glioma-specific Th9 cells induced glioma cell apoptosis in the culture. Treating the glioma-bearing mice with SEB and glioma Ag significantly inhibited the glioma growth. In conclusion, SEB plus glioma Ag immunotherapy inhibits the experimental glioma growth, which may be a novel therapeutic remedy for the treatment of glioma.
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http://dx.doi.org/10.18632/oncotarget.13981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354876PMC
January 2017

Catastrophic health expenditure: a comparative analysis of empty-nest and non-empty-nest households with seniors in Shandong, China.

BMJ Open 2016 07 5;6(7):e010992. Epub 2016 Jul 5.

School of Public Health, Shandong University, Jinan, China.

Objective: The aim of this study was to compare the catastrophic health expenditure (CHE) prevalence and its determinants between empty-nest and non-empty-nest elderly households.

Setting: Shandong province of China.

Participants: A total of 2761 elderly households are included in the analysis.

Results: CHE incidence among elderly households was 44.9%. The CHE incidence of empty-nest singles (59.3%, p=0.000, OR=3.19) and empty-nest couples (52.9%, p=0.000, OR=2.45) are both statistically higher than that of non-empty-nest elderly households (31.4%). An inverse association was observed between CHE incidence and income level in all elderly household types. Factors including 1 or more household elderly members with non-communicable chronic diseases in the past 6 months, 1 or more elderly household members being hospitalised in the past year and lower household income, are significant risk factors for CHE in all 3 household types (p<0.05). Health insurance status was found to be a significant determinant of CHE among empty-nest singles and non-empty-nest households (p<0.05).

Conclusions: CHE incidence among elderly households is high in China. Empty-nest households are at higher risk for CHE than non-empty-nest households. Based on these findings, we suggest that special insurance be developed to broaden the coverage of health services and heighten the reimbursement rate for empty-nest elderly in the existing health insurance schemes. Financial and social protection interventions are also essential for identified at-risk subgroups among different types of elderly households.
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http://dx.doi.org/10.1136/bmjopen-2015-010992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947795PMC
July 2016