Publications by authors named "Liusheng Wang"

6 Publications

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Effect of renal function on the risk of thrombocytopenia in patients receiving linezolid therapy: a systematic review and meta-analysis.

Br J Clin Pharmacol 2021 Jun 30. Epub 2021 Jun 30.

Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Aims: The association of renal function and linezolid-induced thrombocytopenia (LIT) remains controversial. We performed a meta-analysis to determine whether impaired renal function is associated with an increased LIT risk.

Methods: We conducted a systematic search of PubMed, EMBASE and the Cochrane Library from inception to February 2021 for eligible studies evaluating the relationship between renal function and LIT. Indictors of renal function included renal impairment (RI), severe RI, hemodialysis status, creatinine clearance rate (Ccr) and estimated glomerular filtration rate (eGFR). Unadjusted and adjusted estimates and 95% confidence intervals (CIs) were calculated separately using a random-effect model.

Results: A total of 24 studies with 3580 patients were included in the meta-analysis. RI patients had an increased LIT risk compared to non-RI patients in both the unadjusted (OR 4.15; 95% Cl 2.85 to 6.03; I =0%) and adjusted analyses (OR 4.15; 95% Cl 2.85 to 6.03; I =0%). This association persisted in the subset of studies involving only patients receiving a fixed conventional dose (600 mg every 12 h) and other subgroup analyses by ethnicity, sample size and study quality. Moreover, the LIT risk was significantly higher in patients with severe RI and hemodialysis than in patients without severe RI and hemodialysis. The eGFR and Ccr were significantly lower in LIT patients than in non-LIT patients.

Conclusions: Impaired renal function is associated with an increased risk of LIT. A reduced linezolid dose may be considered in RI patients at a low risk of treatment failure, ideally guided by therapeutic drug monitoring.
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http://dx.doi.org/10.1111/bcp.14965DOI Listing
June 2021

Serum exosomal microRNAs as predictive markers for EGFR mutations in non-small-cell lung cancer.

J Clin Lab Anal 2021 May 8;35(5):e23743. Epub 2021 Mar 8.

Department of Infectious Diseases, Affiliated Hangzhou First people's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Current therapeutic drugs show positive effects on non-small-cell lung cancer (NSCLC) patients with mutant epidermal growth factor receptor (EGFR) expression, whereas a lesser beneficial effect is generally noted on NSCLC patients with wild-type EGFR. Therefore, identification of new detection methods for the accurate clinical diagnosis of NSCLC is essential.

Methods: In this study, tumor-derived exosomes from the plasma of EGFR mutation and wild-type NSCLC patients were isolated. Extensive exosomal miRNA profiling of EGFR mutation and wild-type NSCLC patients, in comparison with healthy individuals, was performed using miRNA-sequencing analysis.

Results: The variation of exosomal miRNA expression between control group (NR) and NCSLC samples (AM and AW) was identified. 96 significantly different expressed miRNAs were identified. Of these, 39 miRNAs were upregulated and 57 were downregulated. 11 miRNAs were downregulated, and 31 miRNAs were upregulated in the miRNA expression between NR and AM. Compared with healthy donors, 54 upregulated miRNAs and 36 downregulated miRNAs were observed in samples from AW patients. 40 different expressed miRNAs were identified in AM samples, compared with AW. Ten of upregulated miRNAs are miR-260, miR-1169, miR-117, miR-15b-5p, miRNA-731, miR-342-5p, miR- 898, miR-1384, miR-56, and miR-1214. Ten of downregulated miRNAs are miR-99b-5p, miR-1116, miR-689, miR-818, miR-604, miR-72, miR-955, miR-403, miR-1228, and miR-836.

Conclusion: The exosomal miR-1169 and miR-260 as potential candidates, which contain specific characteristics that can distinguish between wild-type EGFR and mutant EGFR NSCLC patients in early-stage cancers.
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http://dx.doi.org/10.1002/jcla.23743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128312PMC
May 2021

Rapid and precise diagnosis of pulmonary infection in a HIV-negative patient with autosomal-dominant mutation: a case report.

Ther Adv Respir Dis 2020 Jan-Dec;14:1753466620929225

Department of Respiratory Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medical, Hangzhou, Zhejiang, China.

Background: , also named , is an opportunistic pathogen that can cause systemic or limited infection in human beings. This infection is especially common in human immunodeficiency virus (HIV)-infected hosts; however, it has also been recently reported in HIV-negative hosts. Here, we report a very rarely seen case of pulmonary infection in a non-HIV-infected patient with () mutation.

Case Presentation: A 34-year-old woman was admitted to our hospital for uncontrollable nonproductive cough and dyspnea with exercise. She had been immunocompromised since infancy. Computerized tomography scan showed multiple ground glass opacities with multiple bullae in both lungs. Next generation sequencing (NGS) of the bronchoalveolar lavage fluid identified nucleotide sequences. Culture of bronchoscopy specimens further verified the results. The patient was HIV negative, and blood gene detection indicated mutation. To date, following the application of itraconazole, the patient has recovered satisfactorily.

Conclusion: In clinical practice, infection among HIV-negative individuals is relatively rare, and we found that patients who are congenitally immunocompromised due to mutation may be potential hosts. Early diagnosis and timely treatment are expected to improve the prognosis of infection. NGS is a powerful technique that may play an important role in this progress. .
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http://dx.doi.org/10.1177/1753466620929225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7268139PMC
June 2021

Ganoderic acid DM induces autophagic apoptosis in non-small cell lung cancer cells by inhibiting the PI3K/Akt/mTOR activity.

Chem Biol Interact 2020 Jan 23;316:108932. Epub 2019 Dec 23.

Department of Infectious Diseases, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, 310006, Hangzhou, Zhejiang, China. Electronic address:

The incidence and mortality of lung cancer are the highest among cancer-related deaths. However, the long-term use of currently available cytotoxic drugs can increase genetic alterations in cancer cells and cause drug-resistance, which significantly limits their usage. Since current systemic treatment options are limited, effective chemotherapeutic agents are urgently needed for non-small cell lung cancer (NSCLC) treatment. In this study, we demonstrated that ganoderic acid DM (GA-DM) could increase apoptosis in A549 and NCI-H460 NSCLC cells. GA-DM treatment decreased the protein expression levels of Bcl-2 and increased the expression levels of Bax, cleaved caspase-3 and cleaved PRAP. Furthermore, GA-DM could promote autophagic flux, and the cytotoxic effect against cancer cells of GA-DM was significantly inhibited by targeted suppression of autophagy, suggesting that autophagy contributed to GA-DM-induced cell death in NSCLC. Moreover, GA-DM clearly induced autophagy by inactivating the PI3K/Akt/mTOR pathway. When overexpression of Akt reactivated Akt/mTOR pathway in A549 or NCI-H460 cells, the increase of autophagy related marker LC3B-II and apoptosis related protein cleaved PARP and cleaved caspase 3 and the ration of apoptotic cells by GA-DM was reversed, suggesting that GA-DM promoted autophagy and apoptosis by inhibiting Akt/mTOR pathway-mediated autophagy induction. In conclusion, our study indicated that GA-DM can induce autophagic apoptosis in NSCLC by inhibiting Akt/mTOR activity. (209 words).
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http://dx.doi.org/10.1016/j.cbi.2019.108932DOI Listing
January 2020

The Effect of Sweet Taste on Romantic Semantic Processing: An ERP Study.

Front Psychol 2019 9;10:1573. Epub 2019 Jul 9.

Fuzhou Medical College, Nanchang University, Fuzhou, China.

Previous studies have found that sweet perception affects the subjective evaluation of interpersonal intimacy and romantic semantic processing. However, the cognitive processes involved in this effect are unclear. The aim of the current study was to investigate the sweet-love embodied effect in semantic processing and its underlying mechanism by Event-Related potentials technique. Participants were randomly exposed to sweet-taste or tasteless conditions, during which they performed a lexical decision-task that involved romantic and non-romantic words. The results showed an enhanced N400 for romantic words compared to non-romantic words in the sweet-taste condition, and a larger P200 for romantic words relative to non-romantic words. The results demonstrate that taste sensations can cross-modally facilitate the semantic processing of romance. These findings support the embodied effect of sweet-love and are discussed from the perspective of embodied cognition with knowledge activation of concept and semantic richness.
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http://dx.doi.org/10.3389/fpsyg.2019.01573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637307PMC
July 2019

The concreteness effect of word processing for highly neurotic individuals: an event-related potential study.

Neuroreport 2019 03;30(4):305-309

Departments of Psychology.

A number of studies have found a concreteness effect and an abstractness effect in word processing. The triple-component hypothesis proposed that the emotional aspect of words, such as the valence of words, contributes to the abstractness effect. Although there is no direct evidence for the role of affective characteristics of individuals in the abstractness effect, some studies have found a negative bias of highly neurotic individuals. The current study was designed to examine the abstractness effect of words for individuals who were highly neurotic. The event-related potential results showed that highly neurotic individuals exhibited an abstractness effect for negative words on the P300, which was evoked by emotion information, and a concreteness effect for negative words on the N400, which was activated by semantic processing. These results are discussed from the perspective of triple-component hypothesis.
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http://dx.doi.org/10.1097/WNR.0000000000001201DOI Listing
March 2019
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