Publications by authors named "Liu Zheng"

1,709 Publications

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Serum macrophage migration inhibitory factor as a potential biomarker to evaluate therapeutic response in patients with allergic asthma: an exploratory study.

J Zhejiang Univ Sci B 2021 Jun;22(6):512-520

Department of Pulmonary and Critical Care Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

: Previous studies have shown that macrophage migration inhibitory factor (MIF) is involved in the pathogenesis of asthma. This study aimed to investigate whether serum MIF reflects a therapeutic response in allergic asthma. : We enrolled 30 asthmatic patients with mild-to-moderate exacerbations and 20 healthy controls, analyzing the parameter levels of serum MIF, serum total immunoglobulin E (tIgE), peripheral blood eosinophil percentage (EOS%), and fractional exhaled nitric oxide (FeNO). Lung function indices were used to identify disease severity and therapeutic response. : Our study showed that all measured parameters in patients were at higher levels than those of controls. After one week of treatment, most parameter levels decreased significantly except for serum tIgE. Furthermore, we found that serum MIF positively correlated with EOS% as well as FeNO, but negatively correlated with lung function indices. Receiver operator characteristic (ROC) curve analysis indicated that among the parameters, serum MIF exhibited a higher capacity to evaluate therapeutic response. The area under the curve (AUC) of MIF was 0.931, with a sensitivity of 0.967 and a specificity of 0.800. : Our results suggested that serum MIF may serve as a potential biomarker for evaluating therapeutic response in allergic asthma with mild-to-moderate exacerbations.
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http://dx.doi.org/10.1631/jzus.B2000555DOI Listing
June 2021

Deep learning for discovering pathological continuum of crypts and evaluating therapeutic effects: An implication for in vivo preclinical study.

PLoS One 2021 14;16(6):e0252429. Epub 2021 Jun 14.

Global Computational Biology and Digital Sciences, Boehringer Ingelheim Pharmaceuticals, Ridgefield, Connecticut, United States of America.

Applying deep learning to the field of preclinical in vivo studies is a new and exciting prospect with the potential to unlock decades worth of underutilized data. As a proof of concept, we performed a feasibility study on a colitis model treated with Sulfasalazine, a drug used in therapeutic care of inflammatory bowel disease. We aimed to evaluate the colonic mucosa improvement associated with the recovery response of the crypts, a complex histologic structure reflecting tissue homeostasis and repair in response to inflammation. Our approach requires robust image segmentation of objects of interest from whole slide images, a composite low dimensional representation of the typical or novel morphological variants of the segmented objects, and exploration of image features of significance towards biology and treatment efficacy. Both interpretable features (eg. counts, area, distance and angle) as well as statistical texture features calculated using Gray Level Co-Occurance Matrices (GLCMs), are shown to have significance in analysis. Ultimately, this analytic framework of supervised image segmentation, unsupervised learning, and feature analysis can be generally applied to preclinical data. We hope our report will inspire more efforts to utilize deep learning in preclinical in vivo studies and ultimately make the field more innovative and efficient.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252429PLOS
June 2021

Mesh Total Generalized Variation for Denoising.

IEEE Trans Vis Comput Graph 2021 Jun 11;PP. Epub 2021 Jun 11.

Recent study has shown that the Total Generalized Variation (TGV) is highly effective in preserving sharp features as well as smooth transition variations for image processing tasks. However, currently there is no existing work that is suitable for applying TGV to 3D data, in particular, triangular meshes. In this paper, we develop a novel framework for discretizing second-order TGV on triangular meshes. Further, we propose a TGV-based variational method for the denoising of face normal fields on triangular meshes. The TGV regularizer in our method is composed of a first-order term and a second-order term, which are automatically balanced. The first-order term allows our TGV regularizer to locate and preserve sharp features, while the second-order term allows to recognize and recover smoothly curved regions. To solve the optimization problem, we introduce an efficient iterative algorithm based on variable-splitting and augmented Lagrangian method. Extensive results and comparisons on synthetic and real scanning data validate that the proposed method outperforms the state-of-the-art visually and numerically.
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http://dx.doi.org/10.1109/TVCG.2021.3088118DOI Listing
June 2021

Genetic Analysis of a Horizontal Resistance Locus in .

Front Plant Sci 2021 25;12:663868. Epub 2021 May 25.

Department of Plant Science, University of Manitoba, Winnipeg, MB, Canada.

causes blackleg disease in . The blackleg disease is mainly controlled by resistance genes in . Previous studies have shown that the blackleg resistant locus that conferred horizontal resistance under field conditions, is located on chromosome A10 of . The purpose of this study is to fine map this locus and hence identify a candidate gene underlying horizontal resistance. The spectrum of resistance to isolates of the resistance locus was analyzed using near isogenic lines, resistant, and susceptible cultivars. The results showed that this locus was horizontally resistant to all isolates tested. Sequence characterized amplified regions (SCAR), simple sequence repeats (SSR), and single nucleotide polymorphism (SNP) markers were developed in the chromosome region of and a fine genetic map was constructed. Two molecular markers narrowed in a 53.37 kb region where six genes were annotated. Among the six annotated genes, encoding a cytochrome P450 protein were predicted as the candidate of . Based on the profiling of pathogen induced transcriptome, three expressed genes in the six annotated genes were identified while only cytochrome showed upregulation. The candidate corresponds to the gene involved in the indole glucosinolate biosynthesis pathway and plant basal defense in . The molecular markers identified in this study will allow the quick incorporation of the allele in rapeseed cultivars to enhance blackleg resistance.
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http://dx.doi.org/10.3389/fpls.2021.663868DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186441PMC
May 2021

CF33-hNIS-antiPDL1 virus primes pancreatic ductal adenocarcinoma for enhanced anti-PD-L1 therapy.

Cancer Gene Ther 2021 Jun 9. Epub 2021 Jun 9.

Department of Surgery, City of Hope National Medical Center, Duarte, CA, USA.

Immunotherapeutic strategies that combine oncolytic virus (OV) and immune checkpoint inhibitors have the potential to overcome treatment resistance in pancreatic ductal adenocarcinoma (PDAC), one of the least immunogenic solid tumors. Oncolytic viral chimera, CF33-hNIS-antiPDL1 genetically modified to express anti-human PD-L1 antibody and CF33-hNIS-Δ without the anti-PD-L1 gene, were used to investigate the immunogenic effects of OVs and virus-delivered anti-PD-L1 in PDAC in vitro. Western blot, flow cytometry, and immunofluorescence microscopy were used to evaluate the effects of CF33-hNIS-Δ and IFNγ on PD-L1 upregulation in AsPC-1 and BxPC-3 cells, and CF33-hNIS-antiPDL1 production of anti-PD-L1 and surface PD-L1 blockade of AsPC-1 and BxPC-3 with or without cocultured activated T cells. The cytosolic and cell surface levels of PD-L1 in PDAC cell lines varied; only BxPC-3 showed high cell surface expression. Treatment of these cells with CF33-hNIS-Δ and IFNγ significantly upregulated PD-L1 expression and translocation of PD-L1 from the cytosol onto the cell surface. Following coculture of activated T cells and BxPC-3 with CF33-hNIS-antiPDL1, the cell surface PD-L1 blockade on BxPC-3 cells by virus-delivered anti-PD-L1 antibody increased granzyme B release and prevented virus-induced decrease of perforin release from activated CD8+ T cells. Our results suggest that CF33-IOVs can prime immune checkpoint inhibition of PDAC and enhance antitumor immune killing.
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http://dx.doi.org/10.1038/s41417-021-00350-4DOI Listing
June 2021

Proteomic analyses of plasma-derived exosomes in immunoglobulin (Ig) G4-related disease and their potential roles in B cell differentiation and tissue damage.

J Autoimmun 2021 Jun 6;122:102650. Epub 2021 Jun 6.

Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, National Clinical Research Center for Dermatologic and Immunologic Diseases, State Key Laboratory of Complex Severe and Rare Diseases, Beijing, China. Electronic address:

Objective: To investigate the proteomic profiles of plasma exosomes isolated from patients with immunoglobulin (Ig) G4-related disease (IgG4-RD) and to determine their potential roles in B cell differentiation and tissue damage.

Methods: One hundred untreated IgG4-RD patients and 135 sex- and age-matched healthy controls (HCs) were enrolled in this study. A combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and tandem mass tag (TMT)-label quantitation was used for proteomic profiling. Differentially expressed proteins were validated by Western blot, enzyme-linked immunosorbent assay (ELISA) and real-time quantitative PCR (RT-qPCR) analyses. B cell activation, apoptosis, differentiation and reactive oxygen species (ROS) production were analyzed by flow cytometry. We also analyzed the correlations between differentially expressed complement proteins and laboratory parameters.

Results: A total of 178 differentially expressed proteins were identified in plasma exosomes in IgG4-RD patients compared with HCs, and these proteins were enriched predominantly in the complement cascade pathway. Furthermore, reduced expression levels of complement components C3 and C5 in IgG4-RD were correlated with clinical parameters. Following stimulation with IgG4-RD plasma exosomes, the percentages of naïve B cells decreased, while those of memory B cells and plasmablasts increased; the levels of cytochrome c, somatic (CYCS) and downstream complement system activation also increased. Moreover, ROS production was greater in B cells of IgG4-RD patients than in those of HCs. In affected submandibular glands, the BCR signalling pathway was activated, and exosomes were enriched.

Conclusion: Proteomic profiling revealed that plasma exosome proteins may participate in the pathogenesis of IgG4-RD through complement activation and may be involved in B cell differentiation and activation of the B cell auto-oxidative damage pathway.
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http://dx.doi.org/10.1016/j.jaut.2021.102650DOI Listing
June 2021

Chemical Vapor Deposition of Superconducting FeTeSe Nanosheets.

Nano Lett 2021 Jun 9. Epub 2021 Jun 9.

CINTRA CNRS/NTU/THALES, UMI 3288, Research Techno Plaza, Singapore, 637553, Singapore.

FeTeSe, a promising layered material used to realize Majorana zero modes, has attracted enormous attention in recent years. Pulsed laser deposition (PLD) and molecular-beam epitaxy (MBE) are the routine growth methods used to prepare FeTeSe thin films. However, both methods require high-vacuum conditions and polished crystalline substrates, which hinder the exploration of the topological superconductivity and related nanodevices of this material. Here we demonstrate the growth of the ultrathin FeTeSe superconductor by a facile, atmospheric pressure chemical vapor deposition (CVD) method. The composition and thickness of the two-dimensional (2D) FeTeSe nanosheets are well controlled by tuning the experimental conditions. The as-prepared FeTeSe nanosheet exhibits an onset superconducting transition temperature of 12.4 K, proving its high quality. Our work offers an effective strategy for preparing the ultrathin FeTeSe superconductor, which could become a promising platform for further study of the unconventional superconductivity in the FeTeSe system.
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http://dx.doi.org/10.1021/acs.nanolett.1c01577DOI Listing
June 2021

In vivo Sensing of Rabbit Cornea by Terahertz Technology.

J Biophotonics 2021 Jun 9:e202100130. Epub 2021 Jun 9.

Singapore Eye Research Institute, Singapore.

Terahertz (THz) spectroscopy was applied to investigate eight rabbit corneas in vivo. One eye of each rabbits' corneas was edema induced; the other eye of the corneas served as the control. Electromagnetic field simulation shows that THz wave could propagate certain distance and information from cornea sublayers could be reviewed. THz spectra have been collected close to the corneal surface by deviating the direct reflection of the THz beam for the edema cornea, the reflected wave intensity is generally larger compared with the control cornea. Upon edema becomes severe at the end of the observation, the reflected wave intensities obtained by detector corresponding to the corneal deep stroma layer approach to the same value for all observed corneas. Good correlation is observed between central corneal thickness (CCT) measurements and THz wave reflection signal intensities. Our results demonstrated that THz spectroscopy technique could obtain the information from different corneal sublayers. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jbio.202100130DOI Listing
June 2021

Superior mesenteric vessel anatomy features differ in Russian and Chinese patients with right colon cancer: computed tomography-based study.

Chin Med J (Engl) 2021 Jun 7. Epub 2021 Jun 7.

Department of Surgery, Clinic of Colorectal and Minimally Invasive Surgery, I.M. Sechenov First Moscow State Medical University, Moscow 119991, Russia Department of Diagnostic Radiology, National Cancer Centre/National Clinical Research Centre for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China Department of Colorectal Surgery, National Cancer Centre/National Clinical Research Centre for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China.

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http://dx.doi.org/10.1097/CM9.0000000000001566DOI Listing
June 2021

Can artificial intelligence help reduce unnecessary bladder biopsies? Comment on "Assessing treatment response after intravesical bacillus Calmette-Guerin induction cycle: are routine bladder biopsies necessary".

World J Urol 2021 Jun 8. Epub 2021 Jun 8.

Department and Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095Jiefang Avenue, 430030, Wuhan, People's Republic of China.

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http://dx.doi.org/10.1007/s00345-021-03748-9DOI Listing
June 2021

Prognostic value of PTEN in diagnosed metastatic prostate cancer.

Asian J Androl 2021 May 28. Epub 2021 May 28.

Department of Urology, Fudan University Shanghai Cancer Center, Shanghai 200032, China.

The purpose of our study is to investigate the prognostic value of phosphatase and tensin homolog on chromosome 10 (PTEN) expression in patients with de novo metastatic castration naïve prostate cancer (mCNPC). A total of 205 patients with mCNPC at Fudan University Shanghai Cancer Center (Shanghai, China) were retrospectively examined. Immunohistochemical staining of PTEN was performed on prostate biopsy samples of these patients. Associations among clinicopathological features, patient survival and PTEN protein expression were analyzed. PTEN loss occurred in 58 of 205 (28.3%) patients. Loss of PTEN was significantly correlated with high metastatic volume (P = 0.017). No association between PTEN expression and Gleason score was observed. Patients with PTEN loss had significantly shorter progression-free survival (PFS, P < 0.001) and overall survival (OS, P < 0.001) compared with patients with intact PTEN expression. Multivariate analysis showed that elevated alkaline phosphatase, high metastatic volume and PTEN loss were independent poor prognostic factors for PFS. The Eastern Cooperative Oncology Group performance status (ECOG PS) ≥2 and PTEN loss were independent poor prognostic factors for OS. The adjusted hazard ratio of PTEN loss for PFS and OS was 1.67 (95% confidence interval [CI]: 1.14-2.43, P = 0.008) and 1.95 (95% CI: 1.23-3.10, P = 0.005), respectively. PTEN loss was also significantly associated with shorter PFS (P = 0.025) and OS (P < 0.001) in patients with low-volume metastatic disease. Our data showed that PTEN loss is an independent predictor for shorter PFS and OS in patients with de novo mCNPC.
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http://dx.doi.org/10.4103/aja.aja_39_21DOI Listing
May 2021

Second-generation antipsychotics induce cardiotoxicity by disrupting spliceosome signaling: Implications from proteomic and transcriptomic analyses.

Pharmacol Res 2021 Jun 5;170:105714. Epub 2021 Jun 5.

Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China. Electronic address:

Second-generation antipsychotics (SGAs) are first-line drugs that are prescribed for mental disorders in clinic. Severe cardiotoxicity has been widely reported and thus limits their clinical application. This study aimed to identify the common mechanism underlying SGAs-induced cardiotoxicity using dual-omics analyses. Balb/C mice were intraperitoneally injected with two representative SGAs, olanzapine (2.5 mg/kg) and clozapine (25 mg/kg), at clinically comparable doses for 0, 7, 14 and 21 days. Our results showed that both SGAs induced cardiomyocyte degeneration, inflammation infiltration, and cardiac fibrosis, all of which worsened with time. Proteomic analysis revelaed that 22 differentially expressed (DE) proteins overlapped in olanzapine and clozapine-treated hearts. These proteins were significantly enriched in muscle contraction, amino acid metabolism and spliceosomal assembly by GO term analysis and spliceosome signaling was among the top enriched pathways by KEGG analysis. Among the 22 DE proteins, three spliceosome signal proteins were validated in a dynamic detection, and their expression significantly correlated with the extent of SGAs-induced cardiac fibrosis. Following the spliceosome signaling dysregulation, RNA sequencing revealed that alternative splicing events in the mouse hearts were markedly enhanced by SGAs treatments, and the production of vast transcript variants resulted in dysregulation of multiple pathways that are critical for cardiomyocytes adaptation and cardiac remodeling. Pladienolide B, a specific inhibitor of mRNA splicing, successfully corrected SGAs-induced alternative splicing and significantly attenuated the secretion of pro-inflammatory factors and cell deaths induced by SGAs exposure. Our study concluded that the spliceosome signaling was a common pathway driving SGAs cardiotoxicity. Pharmacological inhibition of the spliceosome signaling represents a novel therapeutic strategy against SGAs cardiotoxicity.
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http://dx.doi.org/10.1016/j.phrs.2021.105714DOI Listing
June 2021

Amination of the [email protected] endohedral fullerene: tunable substitution effect on quantum coherence behaviors.

Chem Sci 2020 May 28;11(39):10737-10743. Epub 2020 May 28.

Beijing National Laboratory of Molecular Science, Beijing Key Laboratory of Magnetoelectric Materials and Devices, College of Chemistry and Molecular Engineering, Peking University Beijing 100871 China

The core-shell structure of endohedral fullerene-based anisotropic magnetic molecules of high spin with long coherence time could help scale up quantum systems. In this research, by amination of [email protected] with morpholine, three derivatives are functionalized with 5, 7 and 9 morpholine groups providing an interesting model to investigate the relationship between the quantum coherence and the spin environment. The original radical located on the carbon cage is successfully quenched, affording a quantum phase memory times () over 5 μs at 5 K. By increasing the number of substitution groups, spin-lattice relaxation times () also show significant enhancement due to the interaction variation between the molecules and the environments. We found that the of the three molecules show no obvious difference below 10 K, while they are limited by at higher temperatures. In this work, the variable functional groups are able to tune both and , offering the possibility for application of high-spin magnetic molecules in the field of quantum information processing.
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http://dx.doi.org/10.1039/d0sc02182bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162292PMC
May 2020

Association study of fasting blood glucose and salt sensitivity of blood pressure in community population: The EpiSS study.

Nutr Metab Cardiovasc Dis 2021 May 8. Epub 2021 May 8.

Department of Epidemiology and Health Statistics, School of Public Health, Capital Medical University, and Beijing Municipal Key Laboratory of Clinical Epidemiology Beijing, 100069, China. Electronic address:

Background And Aims: To evaluate the association between fasting blood glucose (FBG) and salt sensitivity of blood pressure (SSBP).

Methods And Results: This study is based on the baseline survey of systemic epidemiology of salt sensitivity study. Subjects were classified into salt sensitive (SS) and salt resistant groups according to blood pressure (BP) changes during the modified Sullivan's acute oral saline load and diuresis shrinkage test. Multivariate logistic and linear regression were used to evaluate associations between FBG with SS or BP changes. A total of 2051 participants were included in the analyses with 581 (28.33%) for SS. Multiple analysis showed that for every interquartile range increase in FBG, the OR (95%CI) for SS was 1.140 (1.069, 1.215), β (95%CI) for mean arterial pressure change (ΔMAP), systolic and diastolic BP changes during saline load were 0.421 (0.221, 0.622), 0.589 (0.263, 0.914) and 0.340 (0.149, 0.531), respectively. Compared to the lowest FBG quartile (Q), the OR (95%CI) for SS in Q and Q were 1.342 (1.014, 1.776) and 1.577 (1.194, 2.084), respectively. Compared to subjects with normal FBG, the β (95%CI) for ΔMAP was 0.973 (0.055, 1.891) in subjects with impaired FBG, and was 1.449 (0.602, 2.296) in patients with diabetes mellitus. Stratified analyses showed significant and stronger associations between FBG with SSBP in youngers, females, hypertensives, non-diabetics, non-current smokers and non-current drinkers.

Conclusion: Our findings suggest FBG is an independent, dose-dependent associated factor for SSBP, and prevention of SS focusing on controlling FBG elevation in the early stage is important.
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http://dx.doi.org/10.1016/j.numecd.2021.04.026DOI Listing
May 2021

Effects of Delayed Mating on Mating Performance and Reproductive Fitness of the Willow Leaf Beetle (Coleoptera: Chrysomelidae) under Laboratory Conditions.

Insects 2021 May 21;12(6). Epub 2021 May 21.

School of Agriculture, Liaocheng University, Liaocheng 252059, China.

Age at mating is one of the most important factors that affect mating selection, sexual performance, and fecundity. We studied the effects of mating age on the mating performance and reproductive fitness of Laicharting, a serious pest of poplar and willow, by measuring the time from pairing to successful mating, mating duration, fecundity, hatching probability, and female lifespan. Delayed mating of females and males significantly prolonged the time from pairing to successful mating and the mating duration, but had no effect on the duration of the egg-laying period. Delayed mating of females did not significantly affect fecundity or egg hatching, but significantly prolonged the female lifespan. Although delayed mating of males had a significant negative impact on egg hatching, it had no effect on the lifespan or fecundity of females. These results indicate that delayed mating affects the mating performance of , although it has a limited effect on reproductive fitness. This suggests that delayed mating is unlikely to contribute to the success of mating disruption, when applied to control .
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http://dx.doi.org/10.3390/insects12060481DOI Listing
May 2021

A reversible shearing DNA probe for visualizing mechanically strong receptors in living cells.

Nat Cell Biol 2021 Jun 31;23(6):642-651. Epub 2021 May 31.

The Institute for Advanced Studies, Wuhan University, Wuhan, China.

In the last decade, DNA-based tension sensors have made significant contributions to the study of the importance of mechanical forces in many biological systems. Albeit successful, one shortcoming of these techniques is their inability to reversibly measure receptor forces in a higher regime (that is, >20 pN), which limits our understanding of the molecular details of mechanochemical transduction in living cells. Here, we developed a reversible shearing DNA-based tension probe (RSDTP) for probing molecular piconewton-scale forces between 4 and 60 pN transmitted by cells. Using these probes, we can easily distinguish the differences in force-bearing integrins without perturbing adhesion biology and reveal that a strong force-bearing integrin cluster can serve as a 'mechanical pivot' to maintain focal adhesion architecture and facilitate its maturation. The benefits of the RSDTP include a high dynamic range, reversibility and single-molecule sensitivity, all of which will facilitate a better understanding of the molecular mechanisms of mechanobiology.
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http://dx.doi.org/10.1038/s41556-021-00691-0DOI Listing
June 2021

Synchronous bone metastasis in lung cancer: retrospective study of a single center of 15,716 patients from Tianjin, China.

BMC Cancer 2021 May 26;21(1):613. Epub 2021 May 26.

Department of Bone and Soft Tissue Tumors, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

Background: This study aimed to describe the incidence, clinical characteristics, and prognosis of lung cancer patients with synchronous bone metastasis (SBM) and to analyze the prognostic factors of the lung cancer patients with SBM.

Methods: A total of 15,716 lung cancer patients who were diagnosed between 2009 to 2018 in the Tianjin Medical University Cancer Institute and Hospital were retrospectively reviewed. Among them, patients with SBM were checked. Both the demographic and clinical characteristics were included as follows: age, gender, marital status, history of smoking, alcohol consumption, family history of tumor, Karnofsky score, lymph node metastasis, histological type. Besides, laboratory data such as alkaline phosphatase, lactate dehydrogenase, carcinoembryonic antigen, squamous cell carcinoma antigen, cytokeratin-19 fragment, and neuron specific enolase were also included. The log-rank test and multivariate Cox regression analysis were employed to reveal the potential prognostic predictors. A further analysis using the Kaplan-Meier was employed to demonstrate the difference on the prognosis of LC patients between adenocarcinoma and non-adenocarcinoma.

Results: Among the included patients, 2738 patients (17.42%) were diagnosed with SBM. A total of 938 patients (34.3%) with SBM were successfully followed and the median survival was 11.53 months (95%CI: 10.57-12.49 months), and the 1-, 2-, and 5-year overall survival rate was 51, 17, and 8%, respectively. Multivariable Cox regression results showed history of smoking and high level of NSE were associated with the poor prognosis, while adenocarcinoma histological type was associated with better survival.

Conclusion: The prevalence of SBM in lung cancer is relatively high with poor survival. The lung cancer patients with SBM showed diverse prognosis. Among all the pathological types, the division of adenocarcinoma suggested different prognosis of the lung cancer patients with SBM. The present study emphasized the importance of pathological diagnosis on prognostic determinants in lung cancer patients with SBM.
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http://dx.doi.org/10.1186/s12885-021-08379-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152068PMC
May 2021

Synergistic effect of carbon starvation and exogenous redox mediators on corrosion of X70 pipeline steel induced by Desulfovibrio singaporenus.

Sci Total Environ 2021 Sep 23;788:147573. Epub 2021 May 23.

Key Laboratory of Marine Environmental Corrosionand Bio-Fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Open Studio for Marine Corrosion and Protection, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China.

In microbiologically influenced corrosion (MIC) induced by sulfate-reducing bacteria (SRB), the electrons released from iron were transferred via extracellular electron transfer (EET) to the inner cells. Electron mediators and carbon starvation have also been found to promote steel corrosion. This study aimed to investigate the synergistic effects of electron mediators and carbon starvation on MIC and their effect on biofilm catalytic activity. The results demonstrated that the weight losses of X70 steel were 0.68 and 1.03 mg/cm in 100% and 10% carbon source (CS) SRB solution, respectively. The addition of riboflavin and cytochrome c increased the corrosion rate by 1.76 and 1.87 times, respectively, in the 100% CS SRB medium compared to the medium without exogenous redox mediators. For the 10% CS SRB medium, the corrosion rate increased by 1.40 and 1.89 times, respectively, when riboflavin and cytochrome c were added. The addition of riboflavin and cytochrome c also enhanced the biocatalytic activity of the SRB biofilm in both the 100% and 10% CS SRB media.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147573DOI Listing
September 2021

Temporal Profiles of Antibody Responses, Cytokines, and Survival of COVID-19 Patients: A Retrospective Cohort in Wuhan, China.

Engineering (Beijing) 2021 May 18. Epub 2021 May 18.

Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

The longitudinal immunologic status of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected patients and its association with the clinical outcome are barely known. Thus, we sought to analyze the temporal profiles of specific antibodies, as well as the correlations between the antibodies, proinflammatory cytokines, and survival of patients with coronavirus disease 2019 (COVID-19). A total of 1830 laboratory-confirmed COVID-19 cases were recruited. The temporal profiles of the virus, antibodies, and cytokines of the patients until 12 weeks since illness onset were fitted by the locally weighted scatter plot smoothing (LOWESS) method. The mediation effect of cytokines on the associations between antibody responses and survival were explored by mediation analysis. Of the 1830 patients, 1435 were detectable for SARS-CoV-2, while 395 were positive in specific antibodies only. Of the 1435 patients, 2.4% presented seroconversion for neither immunoglobulin G (IgG) nor immunoglobulin M (IgM) during hospitalization. The seropositive rates of IgG and IgM were 29.6% and 48.1%, respectively, in the first week, and plateaued within five weeks. For the patients discharged from the hospital, the IgM decreased slowly, while high levels of IgG were maintained at around 188 AU·mL for the 12 weeks since illness onset. In contrast, in the patients who subsequently died, IgM declined rapidly and IgG dropped to 87 AU·mL at the twelfth week. Elevated interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), interleukin-1β (IL-1β), interleukin-2R (IL-2R), and tumor necrosis factor-α (TNF-α) levels were observed in the deceased patients in comparison with the discharged patients, and 12.5% of the association between low IgG level and mortality risk was mediated by these cytokines. Our study deciphers the temporal profiles of SARS-CoV-2-specific antibodies within the 12 weeks since illness onset and indicates the protective effect of antibody response on survival, which may help to guide prognosis estimation.
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http://dx.doi.org/10.1016/j.eng.2021.04.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129785PMC
May 2021

Laparoscopic radical right hemicolectomy with transrectal-specimen extraction: a novel natural-orifice specimen-extraction procedure.

Gastroenterol Rep (Oxf) 2021 Apr 10;9(2):182-184. Epub 2020 Sep 10.

Department of Colorectal Surgery, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P. R. China.

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http://dx.doi.org/10.1093/gastro/goaa047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128022PMC
April 2021

Upregulated insulin receptor tyrosine kinase substrate promotes the proliferation of colorectal cancer cells via the bFGF/AKT signaling pathway.

Gastroenterol Rep (Oxf) 2021 Apr 25;9(2):166-175. Epub 2020 Jul 25.

Department of Colorectal Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, P. R. China.

Background: Some recent studies on insulin receptor tyrosine kinase substrate (IRTKS) have focused more on its functions in diseases. However, there is a lack of research on the role of IRTKS in carcinomas and its mechanism remains ambiguous. In this study, we aimed to clarify the role and mechanism of IRTKS in the carcinogenesis of colorectal cancer (CRC).

Methods: We analysed the expression of IRTKS in CRC tissues and normal tissues by researching public databases. Cancer tissues and adjacent tissues of 67 CRC patients who had undergone radical resection were collected from our center. Quantitative real-time polymerase chain reaction and immunohistochemistry were performed in 52 and 15 pairs of samples, respectively. and experiments were conducted to observe the effect of IRTKS on CRC cells. Gene Set Enrichment Analysis and Metascape platforms were used for functional annotation and enrichment analysis. We detected the protein kinase B (AKT) phosphorylation and cell viability of SW480 transfected with small interfering RNAs (siRNAs) with or without basic fibroblast growth factor (bFGF) through immunoblotting and proliferation assays.

Results: The expression of IRTKS in CRC tissues was higher than that in adjacent tissues and normal tissues (all <0.05). Disease-free survival of patients with high expression was shorter. Overexpression of IRTKS significantly increased the proliferation rate of CRC cells and the number of tumor xenografts . The phosphorylation level of AKT in CRC cells transfected with pLVX-IRTKS was higher than that in the control group. Furthermore, siRNA-IRTKS significantly decreased the proliferation rate of tumor cells and the phosphorylation level of AKT induced by bFGF.

Conclusion: IRTKS mediated the bFGF-induced cell proliferation through the phosphorylation of AKT in CRC cells, which may contribute to tumorigenicity .
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http://dx.doi.org/10.1093/gastro/goaa032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128016PMC
April 2021

Ultrasound Imaging and Antithrombotic Effects of PLA-Combined FeO-GO-ASA Multifunctional Nanobubbles.

Front Med (Lausanne) 2021 4;8:576422. Epub 2021 May 4.

School of Stomatology, Jiamusi University, Jiamusi, China.

PLA-combined ferroferric oxide-graphene oxide-aspirin (FeO-GO-ASA) multifunctional nanobubbles were prepared using the double emulsion-solvent evaporation method. The obtained composite nanobubbles had a regular spherical shape, Zeta potential of (-36.5 ± 10.0) mV, and particle size distribution range of 200-700 nm. The experimental results showed that PLA-combined FeO-GO-ASA nanobubbles could effectively improve the antithrombin parameters of PT, TT, APTT, and INR, and significantly inhibit thrombosis when the composite nanobubbles with a concentration of 80 mg·mL interacted with the rabbit blood. The prepared composite nanobubbles could reach a significant ultrasonic imaging effect and good magnetic targeting under the magnetic field when the nanobubbles' concentration was only 60 mg·mL.
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http://dx.doi.org/10.3389/fmed.2021.576422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129036PMC
May 2021

Fe/FeC Embedded in N-Doped Worm-like Porous Carbon for High-Rate Catalysis in Rechargeable Zinc-Air Batteries.

ACS Appl Mater Interfaces 2021 Jun 20;13(21):24710-24722. Epub 2021 May 20.

College of Materials Science and Engineering, Hunan Province Key Laboratory for Advanced Carbon Materials and Applied Technology, Hunan University, Changsha 410082, China.

Designing low-cost preparation of high-activity electrocatalysts with excellent stability is the route one must take to fully realize large-scale application implementation of zinc-air batteries. 3D nitrogen-doped nanocarbons with transition metals or their derivatives encapsulated in show promising potential in the field of non-precious metal oxygen electrocatalysis. Herein, we report a simple, economical, and large-scale production method to construct worm-like porous nitrogen-doped carbon with -grown carbon nanotubes and uniformly embedded Fe/FeC nanoparticles. It not only has high conductivity owing to the nitrogen-doped nature but also has ample active sites and electrolyte diffusion channels benefitting from the uniformly distributed heterostructural Fe/FeC nanoparticles and discrete hierarchically porous structures. When used as catalyst materials for a zinc-air battery, an energy density of 719.1 Wh kg and a peak power density of 101.3 mW cm at a 50 mA cm discharge current density is achieved. Additionally, throughout charging and discharging for 200 cycles at a current density of 20 mA cm, the charge/discharge voltage gap is nearly constant.
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http://dx.doi.org/10.1021/acsami.1c03220DOI Listing
June 2021

Disorganization of the retinal inner layers as a predictor of visual acuity in eyes with macular edema secondary to uveitis.

Int J Ophthalmol 2021 18;14(5):725-731. Epub 2021 May 18.

Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin 300384, China.

Aim: To assess the correlation between disorganization of the retinal inner layers (DRIL) and best-corrected visual acuity (BCVA) in patients with uveitis and macular edema (UME) who underwent systemic treatment using optical coherence tomography (OCT).

Methods: A retrospective clinical study of 23 patients (30 eyes) with DRIL and 23 patients (31 eyes) without DRIL secondary to UME were included. All patients underwent comprehensive ophthalmic examinations at baseline, 3, 6, and 12mo after local and systemic treatment. The OCT-based parameters included foveal center point thickness (FCPT), mean thickness (MT), and diameters of DRIL in horizontal and vertical directions. BCVA and OCT-based parameters were compared between the two groups. The relationship between each OCT parameter and BCVA was evaluated using linear correlation and regression analysis.

Results: At the initial visit, the mean baseline FCPT was 441.03±128.68 µm in the eyes with DRIL and 337.26±99.31 µm in the eyes without DRIL (=0.001). No significant differences were observed in MT (=0.357). The mean size of transverse and vertical diameters of DRIL was 684.07±267.51 and 267.07±104.61 µm at baseline, respectively. There was significant improvement in BCVA and OCT-based parameters at 3, 6, and 12mo in all cases (<0.001 for each timepoint). In addition, significant differences were detected in BCVA and OCT parameters between eyes with and without DRIL at each time point (<0.01 for each timepoint). A greater DRIL range at baseline was associated with a worse baseline BCVA (transverse diameter of DRIL: =0.875, <0.001; vertical diameter of DRIL: =0.622, <0.001). The transverse diameter of baseline DRIL was found to be significantly correlated with the final BCVA (=0.003).

Conclusion: The improvement in BCVA is associated with DRIL in patients with UME. DRIL is an easy-to-determine and robust imaging biomarker that could help predict BCVA prognosis in eyes with UME.
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http://dx.doi.org/10.18240/ijo.2021.05.13DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077002PMC
May 2021

The willingness for dietary and behavioral changes in frontline epidemic prevention workers after experiencing the outbreak of COVID-19 in China: a cross-sectional study.

Environ Health Prev Med 2021 May 18;26(1):58. Epub 2021 May 18.

Shenzhen Hospital, Southern Medical University, Shenzhen, 518101, China.

Background: The 2019 novel coronavirus disease (COVID-19) has had a massive impact on public health, resulting in sudden dietary and behavioral habit changes. Frontline epidemic prevention workers play a pivotal role against COVID-19. They must face high-risk infection conditions, insufficient anti-epidemic material supplies, mental pressure, and so on. COVID-19 seriously affects their dietary and behavioral habits, and poor habits make them more susceptible to COVID-19. However, their baseline dietary and behavioral habits before COVID-19 and their willingness to change these habits after the outbreak of COVID-19 remain unclear for these workers in China. This study aimed to explore the baseline dietary and behavioral habits of frontline workers and their willingness to change these habits after the outbreak of the epidemic; in addition, susceptible subgroups were identified by stratified analyses as targets of protective measures to keep them from being infected with COVID-19.

Methods: A cross-sectional study was conducted through an online questionnaire using a sample of 22,459 valid individuals living in China, including 9402 frontline epidemic prevention workers.

Results: Before COVID-19, 23.9% of the frontline epidemic prevention workers reported a high-salt diet, 46.9% of them reported a high frequency of fried foods intake, and 50.9% of them smoked cigarettes. After the outbreak of COVID-19, 34.6% of them expressed a willingness to reduce salt intake, and 43.7% of them wanted to reduce the frequency of pickled vegetables intake. A total of 37.9% of them expressed a willingness to decrease or quit smoking, and 44.5% of them wanted to increase sleep duration. Significant differences in the baseline dietary and behavioral habits and the willingness to change their habits were observed between frontline epidemic prevention workers and other participants. Among the frontline epidemic prevention workers with poor dietary and behavioral habits before COVID-19, frontline epidemic prevention experience was a promoting factor for adopting worse dietary and behavioral habits, including those in the high-salt intake subgroup (OR, 2.824; 95% CI, 2.341-3.405) and the 11-20 cigarettes/day subgroup (OR, 2.067; 95% CI, 1.359-3.143).

Conclusions: The dietary and behavioral habits of frontline epidemic prevention workers were worse than that those of other participants before COVID-19. They had a greater willingness to adopt healthy dietary and behavioral habits after experiencing the outbreak of COVID-19. However, frontline epidemic prevention workers with poor dietary and behavioral habits before COVID-19 continued in engage in these poor habits. Dietary and behavioral intervention policies should be drafted to protect their health, especially frontline epidemic prevention workers with poor habits at baseline.
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http://dx.doi.org/10.1186/s12199-021-00979-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130792PMC
May 2021

Dynamical evolution of anisotropic response of type-II Weyl semimetal TaIrTe under ultrafast photoexcitation.

Light Sci Appl 2021 May 14;10(1):101. Epub 2021 May 14.

International Center for Quantum Materials, School of Physics, Peking University, 100871, Beijing, China.

Layered type-II Weyl semimetals, such as WTe, MoTe, and TaIrTe have been demonstrated as a supreme photodetection material with topologically enhanced responsivity and specific sensitivity to the orbital angular momentum of light. Toward future device applications with high performance and ultrafast response, it is necessary to understand the dynamical processes of hot carriers and transient electronic properties of these materials under photoexcitation. In this work, mid-infrared ultrafast spectroscopy is performed to study the dynamical evolution of the anisotropic response of TaIrTe. The dynamical relaxation of photoexcited carriers exhibits three exponential decay components relating to optical/acoustic phonon cooling and subsequent heat transfer to the substrate. The ultrafast transient dynamics imply that TaIrTe is an ideal material candidate for ultrafast optoelectronic applications, especially in the long-wavelength region. The angle-resolved measurement of transient reflection reveals that the reflectivity becomes less anisotropic in the quasi-equilibrium state, indicating a reduction in the anisotropy of dynamical conductivity in presence of photoexcited hot carriers. The results are indispensable in material engineering for polarization-sensitive optoelectronics and high field electronics.
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http://dx.doi.org/10.1038/s41377-021-00546-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121930PMC
May 2021

Hydrostatic pressure effect of photocarrier dynamics in GaAs probed by time-resolved terahertz spectroscopy.

Opt Express 2021 Apr;29(9):14058-14068

Pressure effects on photocarrier dynamics such as interband relaxations and intraband cooling in GaAs have been investigated using in situ time-resolved terahertz spectroscopy with a diamond anvil cell. The interband photocarrier lifetime significantly decreases by nearly two orders of magnitude as the external hydrostatic pressure is increased up to 10 GPa. Considerable pressure tuning for the intervalley scattering processes has also been observed, and the time constants under different pressures are extracted based on the three-state rate model. This work provides new perspectives on tailoring nonequilibrium carrier dynamics in semiconductors using hydrostatic pressure and may serve as the impetus for the development of high-pressure terahertz spectroscopy.
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http://dx.doi.org/10.1364/OE.421011DOI Listing
April 2021

Spatially-directed angiogenesis using ultrasound-controlled release of basic fibroblast growth factor from acoustically-responsive scaffolds.

Acta Biomater 2021 May 5. Epub 2021 May 5.

Department of Radiology, University of Michigan, Ann Arbor, MI, United States; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, United States; Applied Physics Program, University of Michigan, Ann Arbor, MI, United States. Electronic address:

Vascularization is a critical step following implantation of an engineered tissue construct in order to maintain its viability. The ability to spatially pattern or direct vascularization could be therapeutically beneficial for anastomosis and vessel in-growth. However, acellular and cell-based strategies to stimulate vascularization typically do not afford this control. We have developed an ultrasound-based method of spatially- controlling regenerative processes using acellular, composite hydrogels termed acoustically-responsive scaffolds (ARSs). An ARS consists of a fibrin matrix doped with a phase-shift double emulsion (PSDE). A therapeutic payload, which is initially contained within the PSDE, is released by an ultrasound-mediated process called acoustic droplet vaporization (ADV). During ADV, the perfluorocarbon (PFC) phase within the PSDE is vaporized into a gas bubble. In this study, we generated ex situ four different spatial patterns of ADV within ARSs containing basic fibroblast growth factor (bFGF), which were subcutaneously implanted in mice. The PFC species within the PSDE significantly affected the morphology of the ARS, based on the stability of the gas bubble generated by ADV, which impacted host cell migration. Irrespective of PFC, significantly greater cell proliferation (i.e., up to 2.9-fold) and angiogenesis (i.e., up to 3.7-fold) were observed adjacent to +ADV regions of the ARSs compared to -ADV regions. The morphology of the PSDE, macrophage infiltration, and perfusion in the implant region were also quantified. These results demonstrate that spatially-defined patterns of ADV within an ARS can elicit spatially-defined patterns of angiogenesis. Overall, these finding can be applied to improve strategies for spatially-controlling vascularization. STATEMENT OF SIGNIFICANCE: Vascularization is a critical step following implantation of an engineered tissue. The ability to spatially pattern or direct vascularization could be therapeutically beneficial for inosculation and vessel in-growth. However, acellular and cell-based strategies to stimulate vascularization typically do not afford this control. We have developed an ultrasound-based method of spatially-controlling angiogenesis using acellular, composite hydrogels termed acoustically-responsive scaffolds (ARSs). An ARS consists of a fibrin matrix doped with a phase-shift double emulsion (PSDE). An ultrasound-mediated process called acoustic droplet vaporization (ADV) was used to release basic fibroblast growth factor (bFGF), which was initially contained within the PSDE. We demonstrate that spatially-defined patterns of ADV within an ARS can elicit spatially-defined patterns of angiogenesis in vivo. Overall, these finding can improve strategies for spatially-controlling vascularization.
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http://dx.doi.org/10.1016/j.actbio.2021.04.048DOI Listing
May 2021

LncRNA EGOT/miR-211-5p Affected Radiosensitivity of Rectal Cancer by Competitively Regulating ErbB4.

Onco Targets Ther 2021 28;14:2867-2878. Epub 2021 Apr 28.

Department of Colorectal Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.

Background/aims: Long non-coding ribonucleic acids (lncRNAs) are involved in the progression of cancers and affect the response to radiation therapy. This study was to investigate the mechanism of lncRNA EGOT in the radiosensitivity of rectal cancer.

Methods: The mRNA expression of EGOT, miR-211-5p and ErbB4 in rectal cancer tissues and cells was detected by qRT-PCR. The protein expression of ErbB4 was detected by Western blot. Dual-luciferase reporter assay and ribonucleic acid immunoprecipitation (RIP) were used to confirm the interaction between EGOT and miR-211-5p or miR-211-5p and ErbB4. Transfection technology was used to down-regulate and up-regulate the expression of EGOT and miR-211-5p in rectal cancer cells, respectively. MTT, colony formation and flow cytometry were used to detect the effect of EGOT and miR-211-5p on proliferation, invasion, migration and apoptosis of rectal cancer cells.

Results: The expression of EGOT was up-regulated in rectal cancer tissues and cells, and the expression of EGOT was related to the late stage of pathology. EGOT knockdown inhibited the proliferation and colony formation of rectal cancer cells and induced the apoptosis of rectal cancer cells. Moreover, EGOT knockdown was significantly enhanced the effects of radiotherapy on rectal cancer in vivo and in vitro. Furthermore, EGOT was found to serve as a sponge of miR-211-5p, and ErbB4 was a downstream target of miR-211-5p. EGOT enhanced the expression of ErbB4 by regulating miR-211-5p. MiR-211-5p inhibitor restored the effect of EGOT knockdown on the radiosensitivity of rectal cancer.

Conclusion: Down-regulation of EGOT could inhibit the growth of rectal cancer cells by regulating the miR-211-5p/ErbB4 axis and improve the radiosensitivity of rectal cancer cells. EGOT may be a new therapeutic target for rectal cancer.
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http://dx.doi.org/10.2147/OTT.S256989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091867PMC
April 2021