Publications by authors named "Liu Yang"

12,605 Publications

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Visible-blind and flexible metal-semiconductor-metal ultraviolet photodetectors based on sub-10-nm thick silver interdigital electrodes.

Opt Lett 2021 Sep;46(18):4666-4669

We propose and experimentally demonstrate a distinct visible-blind and flexible metal-semiconductor-metal ultraviolet (UV) photodetector (PD) based on sub-10-nm thick silver interdigital electrodes. The transparent PD with 7-nm thick electrodes shows enhanced visible transmittance (80%) in average and achieves greatly improved responsivity (60.5 mA/W), and detectivity (1.75×10 Jones) at 5 V under 380-nm illumination, compared with its opaque counterpart. It is as fast as the opaque PD with short rise and fall times (22.4 and 11.5 ms) and shows good flexibility. All the properties are comparable or superior to many reported transparent UV PDs, mainly attributed to the broad high UV-visible transmittance and the high conductivity of the ultrathin silver film.
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http://dx.doi.org/10.1364/OL.439687DOI Listing
September 2021

Embedded nanometer position tracking based on enhanced phasor analysis: erratum.

Opt Lett 2021 Sep;46(18):4639

We present an erratum to our Letter [Opt. Lett.46, 3825 (2021)OPLEDP0146-959210.1364/OL.433740]. This erratum corrects an error in Eq. (2). All the simulations and experiments in the original Letter were performed using the correct equation, and therefore, this correction does not affect the results and conclusions of the original Letter.
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http://dx.doi.org/10.1364/OL.440217DOI Listing
September 2021

Recent Advances on Bioactive Ingredients of Morchella esculenta.

Appl Biochem Biotechnol 2021 Sep 15. Epub 2021 Sep 15.

Engineering Research Center of Bio-Process, School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, Anhui, China.

Morchella esculenta (M. esculenta) is a delicious edible mushroom prized for its special flavor and strong health promoting abilities. Several bioactive ingredients including polysaccharides, polyphenolic compounds, proteins, and protein hydrolysates all contribute to the biological activities of M. esculenta. Different polysaccharides could be extracted and purified depending on the extraction methods and M. esculenta studied. Monosaccharide composition of M. esculenta polysaccharides (MEP) generally includes mannose, galactose, and glucose, etc. MEP possess multiple bioactivities such as antioxidant, anti-inflammation, immunoregulation, hypoglycemic activity, atherosclerosis prevention and antitumor ability. Other components like polyphenols, protein hydrolysates, and several crude extracts are also reported with strong bioactivities. In terms of potential applications of M. esculenta and its metabolites as nutritional supplements and drug supplements, this review aims to comprehensively summarize the structural characteristics, biological activities, research progress, and research trends of the active ingredients produced by M. esculenta. Among the various biological activities, the substances extracted from both natural collected and submerged fermented M. esculenta are promising for antioxidants, immunomodulation, anti-cancer and anti-inflammatory applications. However, further researches on the extraction conditions and chemical structure of bioactive compounds produced by M. esculenta still need investigations.
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http://dx.doi.org/10.1007/s12010-021-03670-1DOI Listing
September 2021

Glycinebetaine: a versatile protectant to improve rice performance against aluminium stress by regulating aluminium uptake and translocation.

Plant Cell Rep 2021 Sep 15. Epub 2021 Sep 15.

College of Life Sciences, State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271018, Shandong, China.

Key Message: Glycinebetaine alleviates the detrimental effects of aluminium stress by regulating aluminium uptake and translocation, maintaining PSII activity, and activating the oxidative defence, thereby maintaining the growth and development of rice. Aluminium (Al) toxicity is one of the primary growth-limiting factors that limits plant growth and crop productivity in acidic soils. Rice (Oryza sativa L.) plants are susceptible to Al stress and do not naturally accumulate glycinebetaine (GB), one of the most effective protectants. Therefore, the objective of this study was to investigate whether exogenous GB can ameliorate the detrimental effects of Al stress on rice plants. Our results showed that the growth, development and biomass of rice were clearly inhibited under Al stress. However, exogenous GB application increased rice shoot growth and photosynthetic pigments contents, maintained photosystem II (PSII) activity, and activated the antioxidant defence system under Al stress. More importantly, GB may mediate the expression of Al uptake- and translocation-related genes, including OsALS1, OsNrat1, OsSTAR1 and OsSTAR2, and the galacturonic acid contents in rice roots under Al stress. Therefore, our findings highlight exogenous GB application is a valid approach to effectively combat Al toxicity by regulating physiological and biochemical processes in crops.
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http://dx.doi.org/10.1007/s00299-021-02780-8DOI Listing
September 2021

Ginsenoside Rg1 ameliorates aging‑induced liver fibrosis by inhibiting the NOX4/NLRP3 inflammasome in SAMP8 mice.

Mol Med Rep 2021 Nov 15;24(5). Epub 2021 Sep 15.

Key Laboratory of Anti‑Inflammatory and Immunopharmacology, Ministry of Education, Department of Pharmacology, Basic Medicine College, Anhui Medical University, Hefei, Anhui 230032, P.R. China.

Aging is often accompanied by liver injury and fibrosis, eventually leading to the decline in liver function. However, the mechanism of aging‑induced liver injury and fibrosis is still not fully understood, to the best of our knowledge, and there are currently no effective treatment options available for liver aging. Ginsenoside Rg1 (Rg1) has been reported to exert potent anti‑aging effects due to its potential antioxidant and anti‑inflammatory activity. The present study aimed to investigate the protective effect and underlying mechanism of action of Rg1 in aging‑induced liver injury and fibrosis in senescence‑accelerated mouse prone 8 (SAMP8) mice treated for 9 weeks. The histopathological results showed that the arrangement of hepatocytes was disordered, vacuole‑like degeneration occurred in the majority of cells, and collagen IV and TGF‑β1 expression levels, that were detected via immunohistochemistry, were also significantly upregulated in the SAMP8 group. Rg1 treatment markedly improved aging‑induced liver injury and fibrosis, and significantly downregulated the expression levels of collagen IV and TGF‑β1. In addition, the dihydroethylene staining and western blotting results showed that Rg1 treatment significantly reduced the levels of reactive oxygen species (ROS) and IL‑1β, and downregulated the expression levels of NADPH oxidase 4 (NOX4), p47phox, p22phox, phosphorylated‑NF‑κB, caspase‑1, apoptosis‑associated speck‑like protein containing a C‑terminal caspase recruitment domain and the NLR family pyrin domain containing 3 (NLRP3) inflammasome, which were significantly upregulated in the liver tissues of elderly SAMP8 mice. In conclusion, the findings of the present study suggested that Rg1 may attenuate aging‑induced liver injury and fibrosis by reducing NOX4‑mediated ROS oxidative stress and inhibiting NLRP3 inflammasome activation.
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http://dx.doi.org/10.3892/mmr.2021.12441DOI Listing
November 2021

All-optical dynamic analysis of the photothermal and photoacoustic response of a microcantilever by laser Doppler vibrometry.

Photoacoustics 2021 Dec 31;24:100299. Epub 2021 Aug 31.

Laboratory for Soft Matter and Biophysics, Department of Physics and Astronomy, KU Leuven, Leuven, B-3001, Belgium.

Light absorption induced thermoelastic and photoacoustic excitation, combined with laser Doppler vibrometry, was utilized to analyze the dynamic mechanical behavior of a microcantilever. The measured frequency response, modal shapes, and acoustic coupling effects were interpreted in the framework of a simple Bernouilli-Euler model and quantitative 3D finite element method (FEM) analysis. Three opto-mechanical generation mechanisms, each initiated by modulated optical absorption and heating, were identified both by an analytical and finite element model. In decreasing order of importance, optically induced cantilever bending is found to be caused by: (i) differences in photoacoustically induced pressure oscillations in the air adjacent to the illuminated and dark side of the cantilever, resulting from heat transfer from the illuminated cantilever to the nearby air, acting as a volume velocity piston, and (ii) thermoelastic stresses accompanying temperature and thermal expansion gradients in the cantilever, (iii) photoacoustically induced pressure oscillations in the air adjacent to the illuminated cantilever holder and frame.
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http://dx.doi.org/10.1016/j.pacs.2021.100299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426265PMC
December 2021

Sequential different B cell antigen-targeted CAR T-cell therapy for pediatric refractory/relapsed Burkitt Lymphoma.

Blood Adv 2021 Sep 14. Epub 2021 Sep 14.

Department of Hematology/Oncology, National Center for Children's Health, Beijing Children's Hospital, Capital Medical University, Beijing, China, China.

Single antigen-targeted chimeric antigen receptor (CAR) T-cell therapy may be insufficient to induce a durable response in pediatric aggressive B-cell lymphomas. The clinical trial (ChiCTR1800014457) examined the feasibility of sequential different B cell antigen-targeted CAR T-cell therapy for pediatric refractory/relapsed Burkitt lymphoma. Twenty-three patients received the first CD19 CAR T-cell infusion. The patients who did not achieve an ongoing complete response sequentially underwent one or more additional infusions of CAR T-cell targeting CD22 followed by CD20 according to their disease status and CAR T-cell persistence after each infusion. The median time from the last infusion to cutoff date was 17 months (range, 15 to 23). The estimated 18-month complete response rate was 78% (95% confidence interval [CI], 54 to 91). The estimated 18-month progression-free survival rate was 78% (95% CI, 55 to 90), with 78% (95% CI, 37 to 94) in patients with bulky diseases and 60% (95% CI, 25 to 83) in patients with central nervous system (CNS) involvement. During the first CD19 CAR T-cell infusion, grade 3 or higher cytokine release syndrome (CRS) and neurotoxicity occurred in 34.8% and 21.7% of all patients, respectively. During subsequent infusions, few incidences of higher than grade 2 CRS and neurotoxicity were observed. All adverse events were reversible. The severity of neurotoxicity was not significantly different between patients with CNS and non-CNS involvement. Sequential CAR T-cell therapy may result in a durable response and is safe in pediatric refractory/relapsed Burkitt lymphoma. Patients with CNS involvement may benefit from sequential CAR T-cell therapy. This trial was registered at www.chictr.org.cn/index.aspx as ChiCTR1800014457.
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http://dx.doi.org/10.1182/bloodadvances.2021004557DOI Listing
September 2021

Copula-based framework for integrated evaluation of water quality and quantity: A case study of Yihe River, China.

Sci Total Environ 2021 Sep 4;804:150075. Epub 2021 Sep 4.

Key Laboratory of Efficient Irrigation-Drainage and Agricultural Soil-Water Environment in Southern China (Ministry of Education), College of Agricultural Sciences and Engineering, Hohai University, Nanjing 210098, China.

Water quantity and quality are two key factors affecting the performance of integrated watershed management. Conventional water resources assessment of rivers often deals with water quantity and quality separately. However, how to make an objective and impartial assessment of water resources by incorporating both water quantity and quality remains unclear, especially in watersheds with significant human activity impacts and high spatiotemporal variations in flows. In such areas, the nonmonotonic relationship between the water quality and discharge rate of a river, in contrast to near-natural conditions, is often ignored. To resolve this problem, this paper develops a new framework for the integrated evaluation of water quantity and quality by incorporating a new index, namely, the water quality improvement degree (WQID). The WQID is proposed to quantify the disturbance degree of human activities to the near-natural relationship between the water quality and discharge rate of a river. The Yihe River in Northern China is selected as a case study to apply the proposed framework. The results show that the observed flow discharge rates of some abnormal months after a specific time of change-point are greater than the estimated discharges under the river's near-natural condition. The WQID values in these abnormal months are less than 1, resulting in a decrease in the modified water resources surplus (WRS*) or an increase in the modified water resources deficit (WRD*). This indicates that the WQID can take into account the near-natural law between water quantity and quality to make a more objective evaluation of integrated water resources management for the months of interest. The proposed framework can serve as a useful and reliable tool for a comprehensive assessment of the watershed management performance of a river system.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150075DOI Listing
September 2021

Molecular structures of human ACAT2 disclose mechanism for selective inhibition.

Structure 2021 Sep 11. Epub 2021 Sep 11.

Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address:

Endoplasmic reticulum-localized acyl-CoA:cholesterol acyltransferases (ACAT), including ACAT1 and ACAT2, convert cholesterol to cholesteryl esters that become incorporated into lipoproteins or stored in cytosolic lipid droplets. Selective inhibition of ACAT2 has been shown to considerably attenuate hypercholesterolemia and atherosclerosis in mice. Here, we report cryogenic electron microscopy structures of human ACAT2 bound to its specific inhibitor pyripyropene A or the general ACAT inhibitor nevanimibe. Structural analysis reveals that ACAT2 has a topology in membranes similar to that of ACAT1. A catalytic core with an entry site occupied by a cholesterol molecule and another site for allosteric activation of ACAT2 is observed in these structures. Enzymatic assays show that mutations within sites of cholesterol entry or allosteric activation attenuate ACAT2 activity in vitro. Together, these results reveal mechanisms for ACAT2-mediated esterification of cholesterol, providing a blueprint to design new ACAT2 inhibitors for use in the prevention of cardiovascular disease.
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http://dx.doi.org/10.1016/j.str.2021.07.009DOI Listing
September 2021

Beneficial effects of inorganic nitrate in non-alcoholic fatty liver disease.

Arch Biochem Biophys 2021 Sep 11:109032. Epub 2021 Sep 11.

Medical School, University of Western Australia, Perth, WA, Australia; Dobney Hypertension Centre, Medical School, University of Western Australia, Perth, WA, Australia. Electronic address:

Non-alcoholic fatty liver disease (NAFLD) is considered the hepatic representation of the metabolic disorders. Inorganic nitrate/nitrite can be converted to nitric oxide, regulate glucose metabolism, lower lipid levels, and reduce inflammation, thus raising the hypothesis that inorganic nitrate/nitrite could be beneficial for improving NAFLD. This study assessed the therapeutic effects of chronic dietary nitrate on NAFLD in a mouse model. 60 ApoE mice were fed a high-fat diet (HFD) for 12 weeks to allow for the development of atherosclerosis with associated NAFLD. The mice were then randomly assigned to different groups (20/group) for a further 12 weeks: (i) HFD + NaCl (1 mmol/kg/day), (ii) HFD + NaNO (1 mmol/kg/day), and (iii) HFD + NaNO (10 mmol/kg/day). A fourth group of ApoE mice consumed a normal chow diet for the duration of the study. At the end of the treatment, caecum contents, serum, and liver were collected. Consumption of the HFD resulted in significantly greater lipid accumulation in the liver compared to mice on the normal chow diet. Mice whose HFD was supplemented with dietary nitrate for the second half of the study, showed an attenuation in hepatic lipid accumulation. This was also associated with an increase in hepatic AMPK activity compared to mice on the HFD. In addition, a significant difference in bile acid profile was detected between mice on the HFD and those receiving the high dose nitrate supplemented HFD. In conclusion, dietary nitrate attenuates the progression of liver steatosis in ApoE mice fed a HFD.
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http://dx.doi.org/10.1016/j.abb.2021.109032DOI Listing
September 2021

Role of homolog gene of tobacco infertility.

Open Life Sci 2021 24;16(1):815-826. Epub 2021 Aug 24.

Guizhou University College of Agriculture/College of Tobacco, Guizhou Provincial Key Laboratory of Tobacco Quality Research, Guiyang 550025, China.

The sterile line is the basis of crop heterosis utilization. To broaden the sources of male sterility in tobacco, the L. gene was cloned from the tobacco variety K326 by homologous cloning based on the ( L. ) gene sequence of male-sterility genes in pepper. The protein structure and physicochemical properties of the two genes were determined by bioinformatics analysis, and the function of the gene was verified by the CRISPR/Cas9 system. The results showed that the sequences of Ntms1 and Cams1 were 85.25% similar, and plant homeodomains were found in both genes; the physical and chemical properties were also very similar. It is speculated that the gene had the same function as the gene in controlling male sterility. Compared to the wild-type plants, the filaments of the knockout mutant plants were shorter, and the stamen was shorter than the pistil. The anthers did not develop fully and had few viable pollen grains; the tapetum and the anther wall had developed abnormally, and the anther chamber was severely squeezed. The malondialdehyde content in the mutant plants was significantly higher than that in the wild-type plants, while self-fertility was significantly lower in the mutant plants. The results showed that the gene plays an important role in regulating fertility in tobacco.
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http://dx.doi.org/10.1515/biol-2021-0087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386942PMC
August 2021

Facile Synthesis of Holmium-Based Nanoparticles as a CT and MRI Dual-Modal Imaging for Cancer Diagnosis.

Front Oncol 2021 26;11:741383. Epub 2021 Aug 26.

Department of Radiology, The Second Hospital of Jilin University, Changchun, China.

The rapid development of medical imaging has boosted the abilities of modern medicine. As single modality imaging limits complex cancer diagnostics, dual-modal imaging has come into the spotlight in clinical settings. The rare earth element Holmium (Ho) has intrinsic paramagnetism and great X-ray attenuation due to its high atomic number. These features endow Ho with good potential to be a nanoprobe in combined x-ray computed tomography (CT) and T-weighted magnetic resonance imaging (MRI). Herein, we present a facile strategy for preparing HoF nanoparticles (HoF NPs) with modification by PEG 4000. The functional PEG-HoF NPs have good water solubility, low cytotoxicity, and biocompatibility as a dual-modal contrast agent. Currently, there is limited systematic and intensive investigation of Ho-based nanomaterials for dual-modal imaging. Our PEG-HoF NPs provide a new direction to realize and CT/MRI imaging, as well as validation of Ho-based nanomaterials will verify their potential for biomedical applications.
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http://dx.doi.org/10.3389/fonc.2021.741383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427799PMC
August 2021

lncRNA HIF1A-AS2: A potential oncogene in human cancers (Review).

Biomed Rep 2021 Oct 13;15(4):85. Epub 2021 Aug 13.

Department of Laboratory Medicine, The Affiliated Hexian Memorial Hospital of Southern Medical University, Guangzhou, Guangdong 511400, P.R. China.

Long non-coding RNAs (lncRNAs) are transcripts that are >200 nucleotides, but with no open reading frame. An increasing number of lncRNAs have been identified following the development of second-generation sequencing technologies, and they have since become a research hotspot. Functionally, they play a vital role in tumor progression, including in tumor proliferation, migration, invasion, apoptosis and acquisition of drug resistance. They regulate gene expression primarily through interaction with DNA, RNA and proteins at the epigenetic, transcriptional and post-transcriptional levels. Endogenous hypoxia-inducible factor 1α antisense RNA 2 (lncRNA HIF1A-AS2) is aberrantly expressed and involved the development/progression of various types of tumors, such as bladder cancer, glioblastoma, breast cancer and osteosarcoma. It plays a vital role in the proliferation, apoptosis, migration, invasion and epithelial-mesenchymal transformation of various tumor cells. This review summarizes the current body of knowledge on the biological functions and related molecular mechanisms of lncRNA HIF1A-AS2 in the development/progression of human tumors and other diseases.
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http://dx.doi.org/10.3892/br.2021.1461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411487PMC
October 2021

Population Pharmacokinetics of Polymyxin B and Dosage Optimization in Renal Transplant Patients.

Front Pharmacol 2021 25;12:727170. Epub 2021 Aug 25.

Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, China.

Currently, polymyxin B has been widely used in the treatment of multidrug-resistant Gram-negative pathogen infections. Due to the limited pharmacokinetic/pharmacodynamic data, the optimal dosage regimen for the recently proposed therapeutic target of the area under the concentration-time curve over 24 h in steady state divided by the minimum inhibitory concentration 50-100 mg⋅h/L has not yet been established. Moreover, most studies have focused on critically ill patients, yet there have been no studies in the field of renal transplantation. To optimize the dosage strategy and reduce the risk of toxicity, a population pharmacokinetics model of polymyxin B with the Phoenix NLME program was developed in our study. A total of 151 plasma samples from 50 patients were collected in the present study. Polymyxin B plasma concentrations were measured by high-performance liquid chromatography-tandem mass spectrometry. A one-compartment model adequately described the data, and the clearance and volume of distribution were 1.18 L/h and 12.09 L, respectively. A larger creatinine clearance was associated with increased clearance of polymyxin B ( < 0.01). Monte Carlo simulation showed that a regimen of a 75 mg loading dose with a 50 mg maintenance dose was a better option to achieve an optimal therapeutic effect (minimum inhibitory concentration ≤1 mg/L) and to reduce the incidence of side effects for patients with renal impairments. The developed model suggested that dosing adjustment should be based on renal function in renal transplant patients.
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http://dx.doi.org/10.3389/fphar.2021.727170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424097PMC
August 2021

MARCH6 promotes Papillary Thyroid Cancer development by destabilizing DHX9.

Int J Biol Sci 2021 3;17(13):3401-3412. Epub 2021 Aug 3.

Department of Head and Neck Surgical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P. R. China.

Membrane-associated ring-CH-type finger (MARCH) proteins belong to the E3 ubiquitin ligase family, which regulates protein stability by increasing ubiquitination. Recent evidence has shown that some MARCH proteins play important roles in cancer development. However, the role of MARCH6 in tumorigenesis, including thyroid tumorigenesis, remains unknown. In this study, we determined that MARCH6 was upregulated in the majority of primary papillary thyroid cancers (PTCs) at both the mRNA and protein levels. Gain-of-function and loss-of-function studies demonstrated that MARCH6 suppressed apoptosis and promoted cell cycle progression, cell proliferation, growth, migration and tumorigenesis in thyroid cancer cells. Mechanistically, MARCH6 interacted with and downregulated DHX9. Knockdown of DHX9 enhanced the proliferative and migratory abilities of thyroid cancer cells. The inhibitory effect of MARCH6 knockdown on thyroid cancer cell growth and migration was also reversed by DHX9 silencing. In addition, MARCH6 activated the AKT/mTOR signaling pathway in a manner dependent on the downregulation of DHX9. Overall, MARCH6 functions as a potential oncogene in thyroid cancer by destabilizing DHX9 and activating AKT/mTOR signaling.
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http://dx.doi.org/10.7150/ijbs.60628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416720PMC
August 2021

Phylogenetic analyses of the 19 STR loci in X chromosome revealed discriminant forensic characteristics for Chinese Daur and Oroqen minorities.

Int J Legal Med 2021 Sep 12. Epub 2021 Sep 12.

Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin, 150025, Heilongjiang, China.

In respect of forensic genetics, X-STRs are widely applied for deficiency paternity cases. Given the popularization of AGCU-X19 STR Kit in China, there has been investigation conducted into the genetic data and forensic parameters of 19 X-STR loci in many of the Chinese ethnic groups, which makes it possible to perform nationwide phylogenetic comparation. To evaluate the allele and haplotype diversity of 19 X-STR loci and to explore their forensic efficiency in the Daur and Oroqen minorities, unrelated healthy Daur (n = 86) and Oroqen (n = 165) individuals were recruited from Heilongjiang province, so as to reveal the phylogenetic relationship between the two minorities and other Chinese ethnic groups. Of the Daur and Oroqen minorities, 172 and 183 alleles at the 19 X-STR loci were observed, respectively. Haplotype diversity exceeded 0.9 among all the linkage clusters. High cumulative value was observed for the power of discrimination, the probability of exclusion, and the mean exclusion chance for deficiency cases (normal trios and duo cases). As revealed by this study, the panel of 19 X-STR loci is an effective supplementary tool for the kinship test of the studied nationalities.
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http://dx.doi.org/10.1007/s00414-021-02697-7DOI Listing
September 2021

Hydrogen sulfide alleviates salinity stress in Cyclocarya paliurus by maintaining chlorophyll fluorescence and regulating nitric oxide level and antioxidant capacity.

Plant Physiol Biochem 2021 Sep 8;167:738-747. Epub 2021 Sep 8.

Jiyang College, Zhejiang A&F University, Zhuji, Zhejiang, 311800, China; College of Forestry, Nanjing Forestry University, Nanjing, 210037, China. Electronic address:

Cyclocarya paliurus is commonly used to treat diabetes in China. However, the natural habitats of C. paliurus are typically affected by salt stress. Hydrogen sulfide (HS) is a growth regulator that is widely used to enhance plant stress tolerance, but the possible mechanism underlying HS-alleviated salt stress in C. paliurus remains unclear. C. paliurus seedlings pretreated with NaHS (an HS donor) were exposed to salt stress, and then, the leaf and total biomass, chlorophyll fluorescence parameters, nitric oxide (NO) content, oxidative damage, and proline and phenolic content were investigated to test the hypothesis that HS and NO were involved in the salt tolerance of C. paliurus. The results showed that HS pretreatment maintained chlorophyll fluorescence and attenuated the loss of plant biomass. We also found that HS pretreatment further increased the endogenous NO content and nitrate reductase activity compared with salt treatment. Moreover, HS pretreatment alleviated salt-induced oxidative damage, as indicated by lowered lipid peroxidation, through an enhanced antioxidant system including more proline and phenolic accumulation and increased antioxidant enzyme activities. However, C. paliurus leaves treated with the NO scavenger significantly diminished HS-mediated NO production and alleviation of membrane lipid peroxidation. Thus, we concluded that HS-induced NO was involved in C. paliurus salt tolerance.
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http://dx.doi.org/10.1016/j.plaphy.2021.09.004DOI Listing
September 2021

Prevalence of Latent Tuberculosis Infection Among Healthy Young Children and Adolescents and a Two-step Approach for the Diagnosis of Tuberculosis Infection in Chengdu, China.

Pediatr Infect Dis J 2021 Sep 9. Epub 2021 Sep 9.

From the Department of Pediatrics, West China Second University Hospital, Sichuan University; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education; Chengdu Chenghua District Maternal and Child Health Hospital; Department of Pediatrics, Chengdu Second People's Hospital; and Department of Ultrasound, West China Second University Hospital, Sichuan University, Chengdu, China.

Background: China has a high burden of tuberculosis and latent tuberculosis infection (LTBI). The aim of this study was to estimate the prevalence of LTBI among healthy young children and adolescents and test a 2-step approach to explore the threshold for the diagnosis of tuberculosis infection in Chengdu, China.

Methods: Healthy preschool children and school-going children in Chengdu, Sichuan Province, were screened for LTBI using the tuberculin skin test (TST). Preschool children with TST ≥ 5 mm also underwent interferon-γ release assay (IGRA) to explore the threshold of this 2-step approach.

Results: In total, 5667 healthy young children and adolescents completed TST test between July 2020 and January 2021 and were included in the present analysis. The age of the participants ranged from 2.4 to 18 years (median 7.25 ± 4.514 years), of which 2093 (36.9%) were younger than 5 years. The overall prevalence of LTBI was 6.37% and 6.64% in children younger than 5 years old. Fourteen of the 341 preschool children with TST ≥5 mm were interferon-γ release assay positive, of which 4 showed a TST result of 5-10 mm, and 6 preschool children received preventive treatment for LTBI.

Conclusions: Healthy young children and adolescents should also be considered as important target populations for LTBI screening. TST can be recommended for first-line screening as part of a 2-step approach for LTBI screening using a positive threshold of 5 mm.
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http://dx.doi.org/10.1097/INF.0000000000003338DOI Listing
September 2021

Evaluation of retinal sensitivity and microstructure in areas of capillary nonperfusion of eyes with branch retinal vein occlusion.

BMC Ophthalmol 2021 Sep 10;21(1):331. Epub 2021 Sep 10.

Department of Ophthalmology, Peking University First Hospital, No. 8 Xishiku Street, Xicheng District, 100034, Beijing, China.

Background: To evaluate macular microstructure alterations in the parafoveal nonperfusion areas of eyes with branch retinal vein occlusions (BRVO), and to investigate their impact on retinal sensitivity.

Methods: This was a cross-sectional study including thirteen BRVO patients with parafoveal capillary nonperfusion areas (NPA). Multiple modalities including microperimetry, optical coherence tomography angiography, and optical coherence tomography were performed to measure retinal sensitivity and thickness, and to identify the microstructure changes and perfusion status.

Results: The retinal sensitivity and thickness in the NPA were significantly lower than those in the perfusion areas (PA) (P = 0.001, P = 0.003). Microstructure changes, including disorganization of the retinal inner layers (DRIL), disruption of the outer retinal layers, and cysts were more frequently observed in NPA (P = 0.002, P = 0.018, P = 0.068). Within NPA, the retinal sensitivity of areas with DRIL, and outer retinal layers disruption was significantly lower than that of the areas without DRIL (P = 0.016), and with intact outer retinal layers (P < 0.001), respectively. 1dB increase in retinal sensitivity was correlated with 2.2 μm (95 % confidence interval, 1.71-2.7) increase of the thickness (P < 0.001). The retinal sensitivity was significantly lower at points with both DRIL and outer retinal layers disruption than at the points with DRIL or outer retina layers disruption alone (P = 0.001, P = 0.001).

Conclusions: Alterations in the macular microstructure are associated with ischemia, especially DRIL. DRIL and outer retinal layers disruption are imaging features that have important implications for local retinal sensitivity in the ischemic areas, and where the microstructure of both inner and outer retinal layers is disrupted the function is further destructed.
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http://dx.doi.org/10.1186/s12886-021-02089-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434714PMC
September 2021

Effect of Anti-Infective Reconstituted Bone Xenograft Combined with External Fixator on Serum CRP and PCT Levels and Prognosis of Patients with Bone Infection after Lower Extremity Long Bone Trauma.

Evid Based Complement Alternat Med 2021 30;2021:5979514. Epub 2021 Aug 30.

The First People's Hospital of Nantong, The Second Affiliated Hospital of Nantong University, Teaching Hospital Affiliated to Kangda College of Nanjing Medical University, Nantong 226001, Jiangsu, China.

Bone infection is one of the common complications of orthopedic surgery. After bone trauma occurs in the human body, the infection of and Gram-negative bacteria into the fracture area can lead to double infection of the soft tissue and bone tissue at the fracture site, leading to a variety of complications, mostly in the lower extremities. Bone infection easily causes bone destruction, bone nonunion, and bone defect, seriously affecting the quality of life of patients. The traditional treatment method of bone infection is to control the infection first and then repair the bone graft, but this method has a long course, poor efficacy, and high disability rate. In this study, anti-infective reconstituted bone xenograft (ARBX) combined with external fixation was used to treat patients with posttraumatic bone infections of the long bones of the lower extremities, to explore its efficacy, and to analyze its effects on serum CRP, PCT levels, and prognosis. Our results showed that ARBX combined with the external fixator had a good effect on the treatment of patients with bone infection after lower extremity long bone trauma, which could effectively enhance the repair and functional recovery of the limb bone, significantly alleviate the infection degree of patients, reduce the inflammatory response of the body, and have a good prognosis.
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http://dx.doi.org/10.1155/2021/5979514DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423557PMC
August 2021

Clinical Analysis of Video-Assisted Thoracoscopic Surgery for Resection of Solitary Pulmonary Nodules and Influencing Factors in the Diagnosis of Benign and Malignant Nodules.

Evid Based Complement Alternat Med 2021 30;2021:1490709. Epub 2021 Aug 30.

Department of Thoracic Surgery, The First Medical Center of Chinese PLA General Hospital, Beijing 100853, China.

Purpose: This is a retrospective research comparing the clinical outcomes of single-hole versus multi-hole video-assisted thoracoscopic surgical (VATS) resection for solitary pulmonary nodules (SPN) and examining the factors influencing the diagnosis of benign and malignant pulmonary nodules.

Method: We collected the clinical data, surgical status, outcomes, and corresponding imaging features of 317 patients with SPN who were surgically resected by VATS and diagnosed as benign or malignant by pathology in our hospital from January 2019 to December 2021.

Result: Among the 317 patients, 124 (39.12%) underwent single-port VATS and 193 (60.88%) underwent multiple-hole VATS. All patients were grouped according to the different surgical methods, and their postoperative indicators were statistically analyzed. The results showed that neither the single-port VATS group nor the multi-port VATS group had any serious adverse events such as death during the perioperative period. The average operation time, intraoperative blood loss, drainage tube indwelling time, and postoperative hospital stay were significantly lower in the two groups. Statistics of postoperative pathological diagnosis showed that 98 cases (30.91%) of all nodules were benign nodules and 219 cases (69.09%) were malignant nodules, and a further single-multivariate analysis showed that age, nodule maximum diameter, lobular sign, burr sign, vascular cluster sign, and pleural depression sign were independent relevant factors for the diagnosis of benign and malignant nodules.

Conclusion: VATS is less invasive and has fewer complications and is of great clinical value for both diagnosis and treatment of benign and malignant SPN. Age, maximum nodal diameter, lobar sign, burr sign, vascular set sign, and pleural depression sign were independent correlates affecting the diagnosis of benign and malignant SPN, which reminds that great attention should be paid to patients who are older and have risk factors on imaging, and early and timely active treatment or close follow-up should be carried out.
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http://dx.doi.org/10.1155/2021/1490709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423549PMC
August 2021

Biomarkers and Immune Repertoire Metrics Identified by Peripheral Blood Transcriptomic Sequencing Reveal the Pathogenesis of COVID-19.

Front Immunol 2021 24;12:677025. Epub 2021 Aug 24.

Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is a global crisis; however, our current understanding of the host immune response to SARS-CoV-2 infection remains limited. Herein, we performed RNA sequencing using peripheral blood from acute and convalescent patients and interrogated the dynamic changes of adaptive immune response to SARS-CoV-2 infection over time. Our results revealed numerous alterations in these cohorts in terms of gene expression profiles and the features of immune repertoire. Moreover, a machine learning method was developed and resulted in the identification of five independent biomarkers and a collection of biomarkers that could accurately differentiate and predict the development of COVID-19. Interestingly, the increased expression of one of these biomarkers, , a molecule related to nervous system damage, was associated with the clustering of severe symptoms. Importantly, analyses on immune repertoire metrics revealed the distinct kinetics of T-cell and B-cell responses to SARS-CoV-2 infection, with B-cell response plateaued in the acute phase and declined thereafter, whereas T-cell response can be maintained for up to 6 months post-infection onset and T-cell clonality was positively correlated with the serum level of anti-SARS-CoV-2 IgG. Together, the significantly altered genes or biomarkers, as well as the abnormally high levels of B-cell response in acute infection, may contribute to the pathogenesis of COVID-19 through mediating inflammation and immune responses, whereas prolonged T-cell response in the convalescents might help these patients in preventing reinfection. Thus, our findings could provide insight into the underlying molecular mechanism of host immune response to COVID-19 and facilitate the development of novel therapeutic strategies and effective vaccines.
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http://dx.doi.org/10.3389/fimmu.2021.677025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421539PMC
August 2021

Convalescent plasma for hospitalized patients with COVID-19: an open-label, randomized controlled trial.

Nat Med 2021 Sep 9. Epub 2021 Sep 9.

Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.

The efficacy of convalescent plasma for coronavirus disease 2019 (COVID-19) is unclear. Although most randomized controlled trials have shown negative results, uncontrolled studies have suggested that the antibody content could influence patient outcomes. We conducted an open-label, randomized controlled trial of convalescent plasma for adults with COVID-19 receiving oxygen within 12 d of respiratory symptom onset ( NCT04348656 ). Patients were allocated 2:1 to 500 ml of convalescent plasma or standard of care. The composite primary outcome was intubation or death by 30 d. Exploratory analyses of the effect of convalescent plasma antibodies on the primary outcome was assessed by logistic regression. The trial was terminated at 78% of planned enrollment after meeting stopping criteria for futility. In total, 940 patients were randomized, and 921 patients were included in the intention-to-treat analysis. Intubation or death occurred in 199/614 (32.4%) patients in the convalescent plasma arm and 86/307 (28.0%) patients in the standard of care arm-relative risk (RR) = 1.16 (95% confidence interval (CI) 0.94-1.43, P = 0.18). Patients in the convalescent plasma arm had more serious adverse events (33.4% versus 26.4%; RR = 1.27, 95% CI 1.02-1.57, P = 0.034). The antibody content significantly modulated the therapeutic effect of convalescent plasma. In multivariate analysis, each standardized log increase in neutralization or antibody-dependent cellular cytotoxicity independently reduced the potential harmful effect of plasma (odds ratio (OR) = 0.74, 95% CI 0.57-0.95 and OR = 0.66, 95% CI 0.50-0.87, respectively), whereas IgG against the full transmembrane spike protein increased it (OR = 1.53, 95% CI 1.14-2.05). Convalescent plasma did not reduce the risk of intubation or death at 30 d in hospitalized patients with COVID-19. Transfusion of convalescent plasma with unfavorable antibody profiles could be associated with worse clinical outcomes compared to standard care.
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http://dx.doi.org/10.1038/s41591-021-01488-2DOI Listing
September 2021

Facile Fabrication of Hollow Hydrogel Microfiber via 3D Printing-Assisted Microfluidics and Its Application as a Biomimetic Blood Capillary.

ACS Biomater Sci Eng 2021 Sep 10. Epub 2021 Sep 10.

Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510006, China.

Simulating the structure and function of blood capillaries is very important for an in-depth insight into their role in the human body and treatment of capillary-related diseases. Due to the similar composition and structure, hollow hydrogel microfibers are well-recognized as potential biomimetic blood capillaries. In this paper, we report a novel, facile, and reproducible method to fabricate coaxial microfluidic chips via 3D printing-assisted soft lithography and then hollow hydrogel microfibers using the as-prepared coaxial microfluidic chips. Instead of traditional photoresist-based lithography, 3D printing of gelatin hydrogel under various extrusion pressures is used to construct sacrificial templates of coaxial microfluidic chips. Various solid and hollow hydrogel microfibers with complicated and hierarchical structures can be obtained via multitype coaxial microfluidic chips or a combination of coaxial microfluidic fabrication and post-treatment. The as-formed hollow hydrogel microfibers are evaluated in detail as biomimetic blood capillaries, including physicochemical and cytological properties. Our results prove that the hollow hydrogel microfibers exhibit excellent mass transport capacity, hemocompatibility, semipermeability, and mechanical strength, and their barrier function can be further enhanced in the presence of endothelial cells. Overall, our 3D printing-assisted fabrication strategy provides a new technique to construct microfluidic chips with complicated 3D microchannels, and the resulting hollow hydrogel microfibers are promising candidates for blood capillaries.
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http://dx.doi.org/10.1021/acsbiomaterials.1c00980DOI Listing
September 2021

Chelating effect between uranyl and pyridine N containing covalent organic frameworks: A combined experimental and DFT approach.

J Colloid Interface Sci 2021 Aug 24;606(Pt 2):1617-1626. Epub 2021 Aug 24.

MOE Key Laboratory of Resources and Environmental Systems Optimization, College of Environmental Science and Engineering, North China Electric Power University, Beijing 102206, PR China. Electronic address:

Covalent organic frameworks (COFs) are promising adsorbents for removing heavy metal ions, and have high crystallinity, a porous structure, and conjugated stability. N-containing functional groups are known to have great affinity for uranyl ions. In this work, to explore the peculiarity of the pyridine N structure as an efficient adsorbent, we chose 2,2'-dipyridine-5,5'-diamine (Bpy) and pyridine-2,5'-diamine (Py) as the core skeletons, and 1,3,5-triformylphloroglucinol (Tp) as the linker to synthesize two crystalline and stable N-containing COFs named TpBpy and TpPy, respectively, through a facile solvothermal method. Characterization results demonstrated that TpBpy and TpPy possessed regularly growing pore sizes, large specific surface areas and relatively strong thermal resistances. The results of batch experiments showed that both COF materials were capable of the effective removal of uranyl with uptake capacities of 115.45 mg g and 291.79 mg g, respectively. In addition, density functional theory (DFT) simulations highlighted the beneficial chelation effect of the double N structure in pyridine monomers for removing uranyl ions. Combining systematic experimental and theoretical analyses, the adsorption process and interaction mode of porous COFs and UO were revealed, to provide predictable support for the application of pyridine N-containing COFs in the field of environmental remediation.
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http://dx.doi.org/10.1016/j.jcis.2021.08.118DOI Listing
August 2021

Determining the Phase Separation Characteristics of Plant Proteins.

Curr Protoc 2021 Sep;1(9):e237

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Eukaryotic cells contain numerous membrane-bound and membraneless organelles that provide spatiotemporal control for diverse biological processes. The liquid-liquid phase separation of proteins has been proposed as the driving force behind the formation of membraneless organelles. Here, we describe a method to determine the phase separation activities of proteins in plants. This basic method includes protocols for an in vivo fluorescence recovery after photobleaching assay in Nicotiana benthamiana using transient expression, an in vitro liquid droplet reconstitution assay using purified recombinant proteins, and an in vivo fluorescence recovery after photobleaching assay in Arabidopsis thaliana using stable transgenic plants. With these assays, the phase separation characteristics of a protein can be determined. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Detection of protein phase separation activities in N. benthamiana Support Protocol: Fluorescence recovery after photobleaching assay Basic Protocol 2: Detection of protein phase separation in vitro Basic Protocol 3: Detection of protein phase separation in stable transgenic Arabidopsis thaliana plants.
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http://dx.doi.org/10.1002/cpz1.237DOI Listing
September 2021

The interaction between PDCD4 and YB1 is critical for cervical cancer stemness and cisplatin resistance.

Mol Carcinog 2021 Sep 9. Epub 2021 Sep 9.

Institute of Basic Medical Science, Hubei University of Medicine, Shiyan, P. R. China.

Cancer multidrug resistance (MDR) is existence in stem cell-like cancer cells characterized by stemness including high-proliferation and self-renewal. Programmed cell death 4 (PDCD4), as a proapoptotic gene, whether it engaged in cancer stemness and cisplatin resistance is still unknown. Here we showed that PDCD4 expressions in Hela/DDP (cisplatin resistance) cells were lower than in parental Hela cells. Moreover, the levels of drug resistance genes and typical stemness markers were markedly elevated in Hela/DDP cells. In vivo, xenograft tumor assay confirmed that knockdown of PDCD4 accelerated the grafted tumor growth. In vitro, colony formation and MTT assay demonstrated that PDCD4 overexpression inhibited cells proliferation in conditions with or without cisplatin. By contrast, PDCD4 deficiency provoked cell proliferation and cisplatin resistance. On mechanism, PDCD4 decreased the protein levels of pAKT and pYB1, accompanied by reduced MDR1 expression. Correspondingly, luciferase reporter assay showed PDCD4 regulated MDR1 promoter activity entirely relied on YB1. Furthermore, Ch-IP, GST-pulldown, and Co-IP assays provided novel evidence that PDCD4 could directly bind with YB1 by the nucleolar localization signal (NOLS) segment, causing the reduced YB1 binding into the MDR1 promoter region through blocking YB1 nucleus translocation, triggering the decreased MDR1 transcription. Taken together, PDCD4-pAKT-pYB1 forms the integrated molecular network to regulate MDR1 transcription during the process of stemness-associated cisplatin resistance.
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http://dx.doi.org/10.1002/mc.23345DOI Listing
September 2021

Weak Stabilization of Boolean Networks Under State-Flipped Control.

IEEE Trans Neural Netw Learn Syst 2021 Sep 9;PP. Epub 2021 Sep 9.

In this brief, stabilization of Boolean networks (BNs) by flipping a subset of nodes is considered, here we call such action state-flipped control. The state-flipped control implies that the logical variables of certain nodes are flipped from 1 to 0 or 0 to 1 as time flows. Under state-flipped control on certain nodes, a state-flipped-transition matrix is defined to describe the impact on the state transition space. Weak stabilization is first defined and then some criteria are presented to judge the same. An algorithm is proposed to find a stabilizing kernel such that BNs can achieve weak stabilization to the desired state with in-degree more than 0. By defining a reachable set, another approach is proposed to verify weak stabilization, and an algorithm is given to obtain a flip sequence steering an initial state to a given target state. Subsequently, the issue of finding flip sequences to steer BNs from weak stabilization to global stabilization is addressed. In addition, a model-free reinforcement algorithm, namely the Q-learning (QL) algorithm, is developed to find flip sequences to achieve global stabilization. Finally, several numerical examples are given to illustrate the obtained theoretical results.
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http://dx.doi.org/10.1109/TNNLS.2021.3106918DOI Listing
September 2021
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