Publications by authors named "Liqun Zhang"

353 Publications

The formation of phycocyanin-EGCG complex for improving the color protection stability exposing to light.

Food Chem 2021 Aug 30;370:130985. Epub 2021 Aug 30.

State Key Laboratory of Food Nutrition and Safety, College of Food Science and Technology, Tianjin University of Science & Technology, Tianjin 300457, China. Electronic address:

Phycocyanin (PC) is a natural pigment-protein complex in food dye applications. In this study, a phycocyanin-epigallocatechin gallate (EGCG) complex (PE) was prepared and the effects of EGCG on the structure and color stability of PC were evaluated. The fluorescence results showed that the binding number n was 62.1 ± 3.41 (EGCG/PC) and the binding constant K was 4.39 (±0.2) × 10 M, indicating a weak-binding interaction. Fourier transform-infrared analysis showed that EGCG caused structural changes in PC by partially uncoiling α-helix and increasing β-sheet content. The EGCG induced a PC association at a reaction molar ratio above 40:1 (EGCG/PC). Moreover, EGCG protected phycocyanobilin against color fading, making PE more stable relative to PC under 8-days storage in light. This study provides a novel scheme to stabilize PC by forming a complex with polyphenols, which will facilitate the PC application as a natural blue pigment in food.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2021.130985DOI Listing
August 2021

Self-Healing and Elastic Triboelectric Nanogenerators for Muscle Motion Monitoring and Photothermal Treatment.

ACS Nano 2021 Sep 15. Epub 2021 Sep 15.

School of Material Science and Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332-0245, United States.

Owing to wearing and unpredictable damage, the working lifetime of triboelectric nanogenerators (TENGs) is largely limited. In this work, we prepared a single-electrode multifunctional TENG (MF-TENG) that exhibits fast self-healing, human health monitoring capability, and photothermal properties. The device consists of a thin self-healing poly(vinyl alcohol)-based hydrogel sandwiched between two self-healing silicone elastomer films. The MF-TENG exhibits a short-circuit current, short-circuit transfer charge, and open-circuit voltage of 7.98 μA, 78.34 nC, and 38.57 V, respectively. Furthermore, owing to the repairable networks of the dynamic imine bonds in the charged layer and the borate ester bonds in the electrodes, the prepared device could recover its original state after mechanical damage within 10 min at room temperature. The MF-TENG can be attached to different human joints for self-powered monitoring of personal health information. Additionally, the MF-TENG under near-infrared laser irradiation can provide a photothermal therapy for assisting the recovery of human joints motion. It is envisaged that the proposed MF-TENG can be applied to the fields of wearable electronics and health-monitoring devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.1c04384DOI Listing
September 2021

Structural Polymorphism of Chitin and Chitosan in Fungal Cell Walls From Solid-State NMR and Principal Component Analysis.

Front Mol Biosci 2021 25;8:727053. Epub 2021 Aug 25.

Department of Chemistry, Louisiana State University, Baton Rouge, LA, United States.

Chitin is a major carbohydrate component of the fungal cell wall and a promising target for novel antifungal agents. However, it is technically challenging to characterize the structure of this polymer in native cell walls. Here, we recorded and compared C chemical shifts of chitin using isotopically enriched cells of six , , and strains, with data interpretation assisted by principal component analysis (PCA) and linear discriminant analysis (LDA) methods. The structure of chitin is found to be intrinsically heterogeneous, with peak multiplicity detected in each sample and distinct fingerprints observed across fungal species. Fungal chitin exhibits partial similarity to the model structures of α- and γ-allomorphs; therefore, chitin structure is not significantly affected by interactions with other cell wall components. Addition of antifungal drugs and salts did not significantly perturb the chemical shifts, revealing the structural resistance of chitin to external stress. In addition, the structure of the deacetylated form, chitosan, was found to resemble a relaxed two-fold helix conformation. This study provides high-resolution information on the structure of chitin and chitosan in their cellular contexts. The method is applicable to the analysis of other complex carbohydrates and polymer composites.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmolb.2021.727053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423923PMC
August 2021

Biofunctionalization of electrospun fiber membranes by LbL-collagen/chondroitin sulfate nanocoating followed by mineralization for bone regeneration.

Mater Sci Eng C Mater Biol Appl 2021 Sep 3;128:112295. Epub 2021 Jul 3.

State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, PR China; Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address:

It is of great significance to develop osteoinductive artificial scaffold for bone repair and regeneration. We constructed a biomimetic apatite interface on electrospun polycaprolactone fibers by combining layer-by-layer (LbL) nanocoating with mineralization to fabricate an osteoinductive artificial scaffold. After polydopamine modification, cationic type-І collagen and anionic chondroitin sulfate were sequentially adsorbed on the fiber surface. The fibers coated with the multilayer components served as the precursor matrix to induce apatite deposition. By adjusting the number of the layers and duration of mineralization, the nanoscale morphology of composite fibers was optimized. When ten bilayers of the collagen and chondroitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells. In a subcutaneous implantation in mice, this composite fiber membrane enhanced in vivo ectopic osteogenesis. Our nano-architectural scaffolds were able to mimic the composition and structure of the bone matrix to a certain extent, holding great potential for bone repair and regeneration.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.msec.2021.112295DOI Listing
September 2021

Interaction of Human β Defensin Type 3 (hBD-3) with Different PIP2-Containing Membranes, a Molecular Dynamics Simulation Study.

Authors:
Liqun Zhang

J Chem Inf Model 2021 Sep 2. Epub 2021 Sep 2.

Department of Chemical Engineering, Tennessee Technological University, Cookeville, Tennessee 38505, United States.

Human β defensin type 3 (hBD-3) is a cysteine-rich small antibacterial peptide. It belongs to the human innate immune system. hBD-3 has six cysteine residues, which form three pairs of disulfide bonds, and those bonds break in the reducing condition. It is known that hBD-3 can interact with bacterial membrane, and even eukaryotic cell membrane, which has a low concentration of phosphatidylinositol 4,5-bisphosphate (PIP2) lipids. PIP2 is a vital component in cell membranes and has been found to play important roles during antimicrobial peptide (AMP) interaction with membranes. To understand the functional mechanism of hBD-3 interacting with PIP2-containing membranes, the binding structures of hBD-3 on 1-palmitoyl-2-oleoyl--glycero-3-phosphocholine (POPC) bilayers mixed with 10% of PIP2 were predicted using two kinds of methods. The first one is by placing the hBD-3 monomer in different orientations above the POPC + 10%PIP2 membrane to set up five different initial simulation systems and performing long-term simulations on each to predict the most stable binding structure. It was found that hBD-3 analogue binds on the mixed lipid membrane on the two loop regions. The second method is by running long-term simulations on one or nine hBD-3 dimers binding on POPC mixed with 10%PIP2 lipid bilayer starting from the solid-state NMR (ssNMR)-suggested orientation. The dimer dissociated, and the most stable binding of hBD-3 in wild-type on the mixed membrane is also through the two loop regions, which agrees with the prediction from both the first method and the lipid self-assembly result. The PIP2 lipids can form long-lasting hydrogen bonds with positively charged residues such as Arg and Lys on hBD-3, thus forming clusters with hBD-3. As a comparison, hBD-3 dimers binding with a combined bilayer having 1,2-palmitoyl-oleoyl--glycero-3-phosphoserine (POPS) on the upper and POPC on the lower leaflets and the combined POPS + POPC bilayer mixing with 10%PIP2 were also studied. The long-term simulation result shows that hBD-3 can bind with the heads of negatively charged POPS and PIP2 lipids and form hydrogen bonds. The stable binding sites of hBD-3 on PIP2 or POPS mixed bilayers are still on the two loop regions. On the combined POPS + POPC mixed with 10%PIP2 bilayer, the binding of hBD-3 with PIP2 lipids became less stable and fewer because of the competition of binding with the POPS lipids. Besides that, binding with hBD-3 can decrease the membrane thickness of the POPC + PIP2, POPS + POPC, and POPS + POPC + PIP2 bilayers and make POPS and PIP2 lipids more flexible based on the order parameter calculations. Our results supply molecular insight on AMP binding with different membranes and can help understand the functional mechanism of hBD-3 disrupting PIP2-containing membranes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jcim.1c00805DOI Listing
September 2021

Commentary: Craving in Opioid Use Disorder: From Neurobiology to Clinical Practice.

Front Psychiatry 2021 9;12:615921. Epub 2021 Aug 9.

Mental Health Institute of the Second Xiangya Hospital, National Clinical Research Center on Mental Disorders Central South University, Changsha, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyt.2021.615921DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8380841PMC
August 2021

The pentatricopeptide repeat protein EMB1270 interacts with CFM2 to splice specific group II introns in Arabidopsis chloroplasts.

J Integr Plant Biol 2021 Aug 24. Epub 2021 Aug 24.

Shanghai Key Laboratory of Plant Molecular Sciences, Development Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.

Chloroplast biogenesis requires the coordinated expression of chloroplast and nuclear genes. Here, we show that EMB1270, a plastid-localized pentatricopeptide repeat (PPR) protein, is required for chloroplast biogenesis in Arabidopsis thaliana. Knockout of EMB1270 led to embryo arrest, whereas a mild knockdown mutant of EMB1270 displayed a virescent phenotype. Almost no photosynthetic proteins accumulated in the albino emb1270 knockout mutant. By contrast, in the emb1270 knockdown mutant, the levels of ClpP1 and photosystem I (PSI) subunits were significantly reduced, whereas the levels of photosystem II (PSII) subunits were normal. Furthermore, the splicing efficiencies of the clpP1.2, ycf3.1, ndhA, and ndhB plastid introns were dramatically reduced in both emb1270 mutants. RNA immunoprecipitation (RIP) revealed that EMB1270 associated with these introns in vivo. In an RNA electrophoretic mobility shift assay (REMSA), a truncated EMB1270 protein containing the 11 N-terminal PPR motifs bound to the predicted sequences of the clpP1.2, ycf3.1, and ndhA introns. In addition, EMB1270 specifically interacted with CRM Family Member 2 (CFM2). Given that CFM2 is known to be required for splicing the same plastid RNAs, our results suggest that EMB1270 associates with CFM2 to facilitate the splicing of specific group II introns in Arabidopsis. This article is protected by copyright. All rights reserved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jipb.13165DOI Listing
August 2021

Binding free energy calculation of human beta defensin 3 with negatively charged lipid bilayer using free energy perturbation method.

Biophys Chem 2021 Oct 3;277:106662. Epub 2021 Aug 3.

Chemical Engineering Department, Tennessee Technological University, Cookeville, TN 38505, United States of America. Electronic address:

Human β defensin type 3 (hBD-3) is a cationic peptide having strong antimicrobial activities even at high salt concentrations. The conserved sequence is believed to contribute to its unique antibacterial activities. To design novel drugs based on hBD-3, predicting the binding free energy contribution of each residue on hBD-3 with bacterial membrane is important. Firstly, the stable binding structure of hBD-3 dimer in analog form bound on POPG lipid bilayer was predicted using NAMD simulations, which was confirmed by RMSD, buried surface area, hydrogen bonds, distance map, and insertion depth map calculations. Then, free energy perturbation (FEP) method was applied to calculate the binding free energy of each residue by mutating it into Alanine. It was found that the positively charged residues on the tail region of hBD-3 contribute significantly to its binding with membrane. The result emphasized the importance of electrostatic interactions to hBD-3's binding with bacterial membrane.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bpc.2021.106662DOI Listing
October 2021

Cerebral venous thrombosis after vaccination against COVID-19 in the UK: a multicentre cohort study.

Lancet 2021 Aug 6. Epub 2021 Aug 6.

Stroke Service, University Hospitals of North Midlands NHS Trust, Stoke-on-Trent, UK; Faculty of Medicine and Health Sciences, Keele University, Stoke-on-Trent, UK.

Background: A new syndrome of vaccine-induced immune thrombotic thrombocytopenia (VITT) has emerged as a rare side-effect of vaccination against COVID-19. Cerebral venous thrombosis is the most common manifestation of this syndrome but, to our knowledge, has not previously been described in detail. We aimed to document the features of post-vaccination cerebral venous thrombosis with and without VITT and to assess whether VITT is associated with poorer outcomes.

Methods: For this multicentre cohort study, clinicians were asked to submit all cases in which COVID-19 vaccination preceded the onset of cerebral venous thrombosis, regardless of the type of vaccine, interval between vaccine and onset of cerebral venous thrombosis symptoms, or blood test results. We collected clinical characteristics, laboratory results (including the results of tests for anti-platelet factor 4 antibodies where available), and radiological features at hospital admission of patients with cerebral venous thrombosis after vaccination against COVID-19, with no exclusion criteria. We defined cerebral venous thrombosis cases as VITT-associated if the lowest platelet count recorded during admission was below 150 × 10 per L and, if the D-dimer was measured, the highest value recorded was greater than 2000 μg/L. We compared the VITT and non-VITT groups for the proportion of patients who had died or were dependent on others to help them with their activities of daily living (modified Rankin score 3-6) at the end of hospital admission (the primary outcome of the study). The VITT group were also compared with a large cohort of patients with cerebral venous thrombosis described in the International Study on Cerebral Vein and Dural Sinus Thrombosis.

Findings: Between April 1 and May 20, 2021, we received data on 99 patients from collaborators in 43 hospitals across the UK. Four patients were excluded because they did not have definitive evidence of cerebral venous thrombosis on imaging. Of the remaining 95 patients, 70 had VITT and 25 did not. The median age of the VITT group (47 years, IQR 32-55) was lower than in the non-VITT group (57 years; 41-62; p=0·0045). Patients with VITT-associated cerebral venous thrombosis had more intracranial veins thrombosed (median three, IQR 2-4) than non-VITT patients (two, 2-3; p=0·041) and more frequently had extracranial thrombosis (31 [44%] of 70 patients) compared with non-VITT patients (one [4%] of 25 patients; p=0·0003). The primary outcome of death or dependency occurred more frequently in patients with VITT-associated cerebral venous thrombosis (33 [47%] of 70 patients) compared with the non-VITT control group (four [16%] of 25 patients; p=0·0061). This adverse outcome was less frequent in patients with VITT who received non-heparin anticoagulants (18 [36%] of 50 patients) compared with those who did not (15 [75%] of 20 patients; p=0·0031), and in those who received intravenous immunoglobulin (22 [40%] of 55 patients) compared with those who did not (11 [73%] of 15 patients; p=0·022).

Interpretation: Cerebral venous thrombosis is more severe in the context of VITT. Non-heparin anticoagulants and immunoglobulin treatment might improve outcomes of VITT-associated cerebral venous thrombosis. Since existing criteria excluded some patients with otherwise typical VITT-associated cerebral venous thrombosis, we propose new diagnostic criteria that are more appropriate.

Funding: None.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/S0140-6736(21)01608-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346241PMC
August 2021

[Retracted] Synergistic effects of eukaryotic co‑expression plasmid‑based STAT3‑specific siRNA and LKB1 on ovarian cancer and .

Oncol Rep 2021 Sep 23;46(3). Epub 2021 Jul 23.

Center for Reproductive Medicine, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that certain of the western blotting data shown in Figs. 1C and 6D bore unexpected similarities to data appearing in different form in other articles by different authors. Owing to the fact that some of the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to , the Editor has decided that this paper should be retracted from the Journal. The authors agree with the decision to retract the paper. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in 33: 774‑782, 2015; DOI: 10.3892/or.2014.3623].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/or.2021.8149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317160PMC
September 2021

Effects of Add-On Left Ventricular Assist Device to Extracorporeal Membrane Oxygenation During Refractory Cardiac Arrest in a Porcine Model.

ASAIO J 2021 Jul 20. Epub 2021 Jul 20.

From the Department of Heart Disease, Haukeland University Hospital, Bergen, Norway Department of Clinical Science, University of Bergen, Bergen, Norway Centre for Child and Adolescent Mental Health, Eastern and Southern Norway, Oslo, Norway Centre for Violence and Traumatic Stress Studies Oslo, Oslo, Norway Centre for Clinical Research, Haukeland University Hospital, Bergen, Norway Faculty of Medicine, University of Bergen, Bergen, Norway.

This study evaluated the effects of extracorporeal membrane oxygenation (ECMO) in combination with a percutaneous adjunctive left ventricular assist device (LVAD) in a porcine model during 60 minutes of refractory cardiac arrest (CA). Twenty-four anesthetized swine were randomly allocated into three groups given different modes of circulatory assist: group 1: ECMO 72 ml/kg/min and LVAD; group 2: ECMO 36 ml/kg/min and LVAD; and group 3: ECMO 72 ml/kg/min. During CA and extracorporeal cardiopulmonary resuscitation (ECPR), mean left ventricular pressure (mLVP) was lower in group 1 (p = 0.013) and in group 2 (p = 0.003) versus group 3. Mean aortic pressure (mAP) and coronary perfusion pressure (CPP) were higher in group 1 compared with the other groups. In group 3, mean pulmonary artery flow (mPAf) was lower versus group 1 (p = 0.003) and group 2 (p = 0.039). If the return of spontaneous circulation (ROSC) was achieved after defibrillation, up to 180 minutes of unsupported observation followed. All subjects in groups 1 and 3, and 5 subjects in group 2 had ROSC. All subjects in group 1, five in group 2 and four in group 3 had sustained cardiac function after 3 hours of spontaneous circulation. Subjects that did not achieve ROSC or maintained cardiac function post-ROSC had lower mAP (p < 0.001), CPP (p= 0.002), and mPAf (p = 0.004) during CA and ECPR. Add-on LVAD may improve hemodynamics compared with ECMO alone during refractory CA but could not substitute reduced ECMO flow. Increased mAP and CPP could be related to ROSC rate and sustained cardiac function. Increased mLVP was related to poor post-ROSC cardiac function.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MAT.0000000000001528DOI Listing
July 2021

Functional analysis of multiple nifB genes of Paenibacillus strains in synthesis of Mo-, Fe- and V-nitrogenases.

Microb Cell Fact 2021 Jul 19;20(1):139. Epub 2021 Jul 19.

State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.

Background: Biological nitrogen fixation is catalyzed by Mo-, V- and Fe-nitrogenases that are encoded by nif, vnf and anf genes, respectively. NifB is the key protein in synthesis of the cofactors of all nitrogenases. Most diazotrophic Paenibacillus strains have only one nifB gene located in a compact nif gene cluster (nifBHDKENX(orf1)hesAnifV). But some Paenibacillus strains have multiple nifB genes and their functions are not known.

Results: A total of 138 nifB genes are found in the 116 diazotrophic Paenibacillus strains. Phylogeny analysis shows that these nifB genes fall into 4 classes: nifBI class including the genes (named as nifB1 genes) that are the first gene within the compact nif gene cluster, nifBII class including the genes (named as nifB2 genes) that are adjacent to anf or vnf genes, nifBIII class whose members are designated as nifB3 genes and nifBIV class whose members are named as nifB4 genes are scattered on genomes. Functional analysis by complementation of the ∆nifB mutant of P. polymyxa which has only one nifB gene has shown that both nifB1 and nifB2 are active in synthesis of Mo-nitrogenase, while nifB3 and nifB4 genes are not. Deletion analysis also has revealed that nifB1 of Paenibacillus sabinae T27 is involved in synthesis of Mo-nitrogenase, while nifB3 and nifB4 genes are not. Complementation of the P. polymyxa ∆nifBHDK mutant with the four reconstituted operons: nifB1anfHDGK, nifB2anfHDGK, nifB1vnfHDGK and nifB2vnfHDGK, has shown both that nifB1 and nifB2 were able to support synthesis of Fe- or V-nitrogenases. Transcriptional results obtained in the original Paenibacillus strains are consistent with the complementation results.

Conclusions: The multiple nifB genes of the diazotrophic Paenibacillus strains are divided into 4 classes. The nifB1 located in a compact nif gene cluster (nifBHDKENX(orf1)hesAnifV) and the nifB2 genes being adjacent to nif or anf or vnf genes are active in synthesis of Mo-, Fe and V-nitrogenases, but nifB3 and nifB4 are not. The reconstituted anf system comprising 8 genes (nifBanfHDGK and nifXhesAnifV) and vnf system comprising 10 genes (nifBvnfHDGKEN and nifXhesAnifV) support synthesis of Fe-nitrogenase and V-nitrogenase in Paenibacillus background, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12934-021-01629-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287671PMC
July 2021

Innate Immune Anti-Inflammatory Response in Human Spontaneous Intracerebral Hemorrhage.

Stroke 2021 Jul 20:STROKEAHA121034673. Epub 2021 Jul 20.

Molecular and Clinical Sciences Research Institute, St George's University of London, United Kingdom (A.S., L.R.B., A.C.P., A.H.H.).

Background And Purpose: Spontaneous intracerebral hemorrhage (sICH) is a common form of hemorrhagic stroke, with high mortality and morbidity. Pathophysiological mechanisms in sICH are poorly understood and treatments limited. Neuroinflammation driven by microglial-macrophage activation contributes to brain damage post-sICH. We aim to test the hypothesis that an anti-inflammatory (repair) process occurs in parallel with neuroinflammation in clinical sICH.

Methods: We performed quantitative analysis of immunohistochemical markers for microglia and macrophages (Iba1, CD68, TMEM119, CD163, and CD206) in brain tissue biospecimens 1 to 12 days post-sICH and matched control cases. In a parallel, prospective group of patients, we assayed circulating inflammatory markers (CRP [C-reactive protein], total white cell, and monocyte count) over 1 to 12 days following sICH.

Results: In 27 supratentorial sICH cases (n=27, median [interquartile range] age: 59 [52-80.5], 14F/13M) all microglia-macrophage markers increased post-sICH, relative to control brains. Anti-inflammatory markers (CD163 and CD206) were elevated alongside proinflammatory markers (CD68 and TMEM119). CD163 increased progressively post-sICH (15.0-fold increase at 7-12 days, <0.001). CD206 increased at 3 to 5 days (5.2-fold, <0.001) then returned to control levels at 7 to 12 days. The parenchymal immune response combined brain-derived microglia (TMEM119 positive) and invading monocyte-derived macrophages (CD206 positive). In a prospective sICH patient cohort (n=26, age 74 [66-79], National Institutes of Health Stroke Scale on admission: 8 [4-17]; 14F/12M) blood CRP concentration and monocyte density (but not white blood cell) increased post-sICH. CRP increased from 0 to 2 to 3 to 5 days (8.3-fold, =0.020) then declined at 7 to 12 days. Monocytes increased from 0 to 2 to 3 to 5 days (1.8-fold, <0.001) then declined at 7 to 12 days.

Conclusions: An anti-inflammatory pathway, enlisting native microglia and blood monocytes, occurs alongside neuroinflammation post-sICH. This novel pathway offers therapeutic targets and a window of opportunity (3-5 days post-sICH) for delivery of therapeutics via invading monocytes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1161/STROKEAHA.121.034673DOI Listing
July 2021

N6-methyladenosine Demethylase FTO Induces the Dysfunctions of Ovarian Granulosa Cells by Upregulating Flotillin 2.

Reprod Sci 2021 Jul 12. Epub 2021 Jul 12.

Department of Gynecology, The First Hospital of Jilin University, No. 71, Xinmin Street, Changchun, 130021, Jilin, China.

Polycystic ovarian syndrome (PCOS) is often accompanied by overweight/obesity and insulin resistance. The dysfunctions of ovarian granulosa cells (GCs) are closely linked with the pathogenesis of PCOS. Fat mass and obesity-associated gene (FTO), an N6-methyladenosine (m6A) demethylase, has been reported to be implicated in the risks and insulin resistance of PCOS. However, the roles of FTO in the development of GCs along with its m6A-related regulatory mechanisms are poorly defined. Cell proliferative ability was detected by MTT assay. Cell apoptotic rate was measured via flow cytometry. Insulin resistance was assessed by GLUT4 transport potential. The mRNA and protein levels of FTO and flotillin 2 (FLOT2) were determined by RT-qPCR and western blot assays, respectively. FLOT2 was screened out to be a potential FTO target through differential expression analysis for the GSE95728 dataset and target prediction analysis by POSTAR2 and STARBASE databases. The interaction between FTO and FLOT2 was analyzed by RNA immunoprecipitation (RIP) assay. The effect of FTO upregulation on FLOT2 m6A level was measured by methylated RIP (meRIP) assay. FLOT2 mRNA stability was examined by actinomycin D assay. FTO overexpression facilitated cell proliferation, hindered cell apoptosis, and induced insulin resistance in GCs. FTO promoted FLOT2 expression by reducing m6A level on FLOT2 mRNA and increasing FLOT2 mRNA stability. FLOT2 loss weakened the effects of FTO overexpression on cell proliferation/apoptosis and insulin resistance in GCs. FTO induced the dysfunctions of GCs by upregulating FLOT2, suggesting that FTO/FLOT2 might play a role in the pathophysiology of PCOS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s43032-021-00664-6DOI Listing
July 2021

Prognostic Value of Albumin to D-Dimer Ratio in Advanced Gastric Cancer.

J Oncol 2021 21;2021:9973743. Epub 2021 Jun 21.

Medical Oncology Department of Gastrointestinal Cancer, Liaoning Cancer Hospital & Institute, Cancer Hospital of China Medical University, No. 44 Xiaoheyan Road, Dadong District, Shenyang 110042, Liaoning Province, China.

Gastric cancer (GC) is one of the most common malignancies worldwide. Notably, patients with advanced GC have a poor prognosis and quality of life, prompting the need for further studies on its prognostic markers. Among these, albumin and D-dimer are often used as prognostic factors in the prediction of a variety of tumors. Moreover, the albumin to D-dimer ratio (ADR) may be an improved predictor of chemotherapy effect and survival compared to albumin and D-dimer alone, but few studies have investigated this issue. Thus, we explored the relationship between pretreatment ADR and prognosis in advanced GC treated with first-line chemotherapy. A total of 247 advanced unresectable GC patients treated with first-line chemotherapy were retrospectively included. The cut-off value for ADR was determined using the receiver operating characteristic (ROC) curve. The ADR had a cut-off value of 41.64. Compared to albumin and D-dimer alone, ADR had the highest area under curve (AUC) value (AUC = 0.730), followed by albumin (AUC = 0.659) and D-dimer (AUC = 0.719). Additionally, we found that patients with a low ADR (<41.64) had a lower disease control rate (77.9% vs. 92.5%, < 0.01), shorter overall survival (OS) (271 vs. 389 days), and shorter progression-free survival (PFS) (118 vs. 192 days) than patients with a high ADR (≥41.64). Similar results were also found on subgroup analysis, and ADR was found to be an independent advanced GC prognostic factor on multivariate analysis (all < 0.001). Low ADR was found to be correlated with poor therapeutic effects of chemotherapy and shortened OS and PFS. Therefore, pretreatment ADR may be a useful tool for predicting the effect of chemotherapy and prognosis in advanced GC patients treated with first-line chemotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/9973743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241521PMC
June 2021

Percolated Network of Mixed Nanoparticles with Different Sizes in Polymer Nanocomposites: A Coarse-Grained Molecular Dynamics Simulation.

Materials (Basel) 2021 Jun 15;14(12). Epub 2021 Jun 15.

State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China.

The size of real nanoparticles (NPs) is polydisperse which can influence the electrical property of polymer nanocomposites (PNCs). Here, we explored the percolated network of mixed NPs with different sizes (small NPs and big NPs) by adopting a molecular dynamics simulation. The simulated results reveal that the big NPs are adverse to building the percolated network compared to the small NPs. Thus, the percolation threshold becomes higher along with increasing the mixing ratio, which denotes the concentration ratio of big NPs to the total NPs. For a better understanding of it, the dispersion state and the number and the size of clusters are employed to analyze the percolated network, which can explain the percolation threshold well. Furthermore, by adopting the Sun's theory (Macromolecules, 2009, 42, 459-463), small and big NPs exhibit a weak antagonistic effect in the simulation if their total concentration is fixed. On the one hand, the number of small NPs is larger than that of big NPs at the same concentration. In addition, one big NP can connect to more others than one small NP. These two contrast effects are responsible for it. Interestingly, the shear flow leads to more contact aggregation structure of NPs which is beneficial to build the new percolated networks. Especially, the big NPs play a more important role in forming the percolated network than small NPs. Consequently, the percolation threshold is reduced at a higher shear rate. In total, our research work provides a further understanding of how the mixed NPs with different sizes form the percolated network in polymer matrix.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma14123301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232767PMC
June 2021

Determination of 15 + 1 European Priority Polycyclic Aromatic Hydrocarbons in Smoked Meat Products by Saponification/Solid-Phase Extraction and Gas Chromatography-Mass Spectrometry.

J Chromatogr Sci 2021 Jun 24. Epub 2021 Jun 24.

Center for Disease Control and Prevention of Zhejiang Province, Hangzhou 310009, P. R. China.

A method for the determination of 15 + 1 European priority polycyclic aromatic hydrocarbons (EUPAHs) in smoked meat samples by saponification/solid-phase extraction and gas chromatography-mass spectrometry has been developed. Both saponification and solid-phase extraction conditions were optimized, which lead to shorter sample preparation time and excellent sensitivity and selectivity. The optimal saponification condition for the lipid extract of 5.00 g smoked food sample was 5 mL KOH (1.5 mol/L)-ethanol at 70°C for 5 min, and the shorter alkaline treatment time avoided the loss of volatile EUPAHs such as Benzo[c]fluorene. All the EUPAHs showed good linearity in the range between 5.0 and 50.0 ng/mL with correlation coefficients between 0.997 and 1.00. The estimated LODs for the EUPAHs were 0.15-0.30 μg/kg, while the LOQs were 0.50-1.0 μg/kg. The three spiking levels of EUPAHs were 1.0, 2.0 and 5.0 μg/kg, and the average recovery was between 75.2 and 99.6%, while the RSD were 2.3-12.4%. This sensitive and rapid method was successfully applied to smoked meat samples from Zhejiang Province of China, and the results revealed the presence of 13 EUPAHs. Benzo[a]pyrene (BaP) was found in 19 out of 20 samples, with concentration ranging from 0.51 to 4.57 μg/kg. The sum of concentrations of PAH4 (summation of benzo(a)pyrene, chrysene, benzo(a)anthracene, and benzo(b)fluoranthene) were 2.40-53.56 μg/kg.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/chromsci/bmab087DOI Listing
June 2021

Metoprolol attenuates intracerebral hemorrhage-induced cardiac damage by suppression of sympathetic overactivity in mice.

Auton Neurosci 2021 Sep 5;234:102832. Epub 2021 Jun 5.

Department of Neurology, Tianjin Medical University General Hospital, Tianjin Neurological Institute, Key Laboratory of Post-Neurotrauma, Neurorepair, and Regeneration in Central Nervous System, Ministry of Education and Tianjin City, Tianjin 300052, China. Electronic address:

The high rates of mortality and disability resulting from intracerebral hemorrhage (ICH) are closely related to subsequent cardiac complications. The mechanisms underlying ICH-induced cardiac dysfunction are not fully understood. In this study, we investigated the role of sympathetic overactivity in mediating cardiac dysfunction post ICH in mice. Collagenase-injection ICH model was established in adult male C57BL/6J mice. Neurological function was subsequently evaluated at multiple time points after ICH and cardiac function was measured by echocardiography on 3 and 14 days after ICH. Plasma adrenaline, noradrenaline, cortisol and heart β1 adrenergic receptor (β1-AR) levels were assessed to evaluate sympathetic activity. Picro Sirius Red (PSR) staining was performed to evaluate cardiomyocyte hypertrophy and interstitial fibrosis. Monocyte chemotactic protein 1 (MCP-1), tumor necrosis factor-alpha (TNF-α), interleukin-6(IL-6), nuclear factor kappa-B(NF-κB), NADPH oxidase-2 (NOX2), matrix metalloprotein (MMP-9) and transforming growth factor-beta (TGF-β) levels were assessed to evaluate inflammation, fibrosis and oxidative stress levels in heart after ICH. Macrophages and neutrophils were assessed to evaluate inflammatory cell infiltration in heart after ICH. ICH induced sympathetic excitability, as identified by increased circulating adrenaline, noradrenaline, cortisol levels and β1-AR expression in heart tissue. Metoprolol-treated ICH mice had improved cardiac and neurological function. The suppression of sympathetic overactivity by metoprolol attenuates cardiac inflammation, fibrosis and oxidative stress after ICH. In conclusion, ICH-induced secondary sympathetic overactivity which mediated inflammatory response may play an important role in post-ICH cardiac dysfunction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.autneu.2021.102832DOI Listing
September 2021

Sudden deafness as an initial presentation of varicella: case report and literature review.

Ann Palliat Med 2021 May;10(5):5891-5896

Department of Otorhinolaryngology, The Second Affiliated Hospital of Shanghai University (Wenzhou Central Hospital), Wenzhou, China.

Varicella, or chickenpox, is a highly contagious disease transmitted by the varicella zoster virus (VZV). Idiopathic sudden sensorineural hearing loss (ISSNHL) has been defined as a loss of at least 30 dB over at least three frequencies on audiogram within three days and with an uncertain cause. Deafness caused by varicella-zoster virus occasionally occurred in Ramsey hunter syndrome but associated with chickenpox is rare. We present a case of an adult varicella infection with sudden deafness as the first symptom. The patient begins with a sudden hearing loss in his left ear and developed fever 12 days later and skin rash 13 days later. He was diagnosed as chickenpox and received treatment of oral famciclovir capsule 250 mg administrated three times daily. After 2 weeks, at an outpatient follow-up the patient reported that while his scabs had fallen off and he felt well, the sudden deafness in the left ear had not improved. This kind of cases are rare and suggest the relationship between VZV and sudden deafness. Sudden deafness can be the first symptom of chickenpox. In this study, we also reviewed pathogenesis, clinical feature and the epidemiological character of chickenpox, the neurological complications of varicella zoster and the etiology of sudden deafness.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/apm-21-785DOI Listing
May 2021

Molecular Dynamics Simulations of Human Beta-Defensin Type 3 Crossing Different Lipid Bilayers.

ACS Omega 2021 Jun 18;6(21):13926-13939. Epub 2021 May 18.

Chemical Engineering department, Tennessee Technological University, 1 William L Jones Dr, Cookeville, Tennessee 38505, United States.

Human β defensin type 3 (hBD-3) is a small cationic cysteine-rich peptide. It has a broad spectrum of antimicrobial activities. However, at high concentrations, it also shows hemolytic activity by interrupting red blood cells. To understand the selectivity of hBD-3 disrupting cell membranes, investigating the capability of hBD-3 translocating through different membranes is important. Since hBD-3 in the analogue form in which all three pairs of disulfide bonds are broken has similar antibacterial activities to the wild-type, this project investigates the structure and dynamics of an hBD-3 analogue in monomer, dimer, and tetramer forms through both zwitterionic and negatively charged lipid bilayers using molecular dynamics (MD) simulations. One tetramer structure of hBD-3 was predicted by running all-atom MD simulations on hBD-3 in water at a high concentration, which was found to be stable in water during 400 ns all-atom simulations based on root-mean-squared deviation, root-mean-squared fluctuation, buried surface area, and binding interaction energy calculations. After that, hBD-3 in different forms was placed inside different membranes, and then steered MD simulation was conducted to pull the hBD-3 out of the membrane along the -direction to generate different configurational windows to set up umbrella-sampling (US) simulations. Because extensive sampling is important to obtain accurate free energy barriers, coarse-grained US MD simulations were performed in each window. Based on the long-term simulation result, membrane thinning was found near hBD-3 in different lipid bilayers and in different hBD-3 oligomer systems. By calculating the root-mean-squared deviation of the -coordinate of hBD-3 molecules, rotation of the oligomer inside the bilayer and stretching of the oligomer structure along the -direction were observed. Although reorientation of lipid heads toward the hBD-3 tetramer was observed based on the density profile calculation, the order parameter calculation shows that hBD-3 disrupts 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS) lipids more significantly and makes it less ordered than on 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipids. Calculating the free energy of hBD-3 through different lipid bilayers, it was found that generally hBD-3 encounters a lower energy barrier through negatively charged lipid membranes than the zwitterionic membrane. hBD-3 in different forms needs to overcome a lower energy barrier crossing the combined POPC+POPS bilayer through the POPS leaflet than through the POPC leaflet. Besides that, the potential of mean force result suggests that hBD-3 forms an oligomer translocating negatively charged lipid membranes at a low concentration. This study supplied new insight into the antibacterial mechanism of hBD-3 through different membranes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.1c01803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173616PMC
June 2021

Breathable TiCT MXene/Protein Nanocomposites for Ultrasensitive Medical Pressure Sensor with Degradability in Solvents.

ACS Nano 2021 06 3;15(6):9746-9758. Epub 2021 Jun 3.

Interdisciplinary Research Center for Artificial Intelligence, College of Materials Science and Engineering, State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China.

Flexible, breathable, and degradable pressure sensors with excellent sensing performance are drawing tremendous attention for various practical applications in wearable artificial skins, healthcare monitoring, and artificial intelligence due to their flexibility, breathability, lightweight, decreased electronic rubbish, and environmentally friendly impact. However, traditional plastic or elastomer substrates with impermeability, uncomfortableness, mechanical mismatches, and nondegradability greatly restricted their practical applications. Therefore, the fabrication of such pressure sensors with high flexibility, facile degradability, and breathability is still a critical challenge and highly desired. Herein, we present a wearable, breathable, degradable, and highly sensitive MXene/protein nanocomposites-based pressure sensor. The fabricated MXene/protein-based pressure sensor is assembled from a breathable conductive MXene coated silk fibroin nanofiber (MXene-SF) membrane and a silk fibroin nanofiber membrane patterned with a MXene ink-printed (MXene ink-SF) interdigitated electrode, which can serve as the sensing layer and the electrode layer, respectively. The assembled pressure sensor exhibits a wide sensing range (up to 39.3 kPa), high sensitivity (298.4 kPa for 1.4-15.7 kPa; 171.9 kPa for 15.7-39.3 kPa), fast response/recovery time (7/16 ms), reliable breathability, excellent cycling stability over 10 000 cycles, good biocompatibility, and robust degradability. Furthermore, it shows great sensing performance in monitoring human psychological signals, acting as an artificial skin for the quantitative illustration of pressure distribution, and wireless biomonitoring in real time. Considering the biodegradable and breathable features, the sensor may become promising to find potential applications in smart electronic skins, human motion detection, disease diagnosis, and human-machine interaction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.1c00472DOI Listing
June 2021

Succinylated ferritin as a novel nanocage-like vehicle of polyphenol: Structure, stability, and absorption analysis.

Food Chem 2021 Nov 12;361:130069. Epub 2021 May 12.

State Key Laboratory of Food Nutrition and Safety, College of Food Science and Technology, Tianjin University of Science & Technology, Tianjin 300457, China. Electronic address:

Ferritin, a protein with an 8-nm cage structure, can encapsulate and deliver bioactive molecules. In this study, succinylation was adopted to modify plant ferritin to fabricate succinylated red been ferritin (SRBF) at pH 8.0. The SRBF was retained as a cage-like shape (12 nm diameter), while its secondary structure was altered, rendering higher negative charge accompanies by decreased surface hydrophobicity. The SRBF also demonstrated favorable property of reversible assembly regulated by pH-transitions (pH 2.0/7.0), thus enabled successful encapsulation of epigallocatechin gallate (EGCG) for fabrication of EGCG-loaded SRBF complexes with a diameter of ~12 nm. Succinylation enhanced the thermal stabilities of ferritin and the embedded EGCG. Moreover, SRBF markedly improved the transport efficiency of EGCG in Caco-2 monolayers relative to EGCG and that encapsulated in unmodified ferritin. These findings have extended the succinylation reaction for the cage-like protein modification, and facilitated the usage of ferritin variant in delivery of bioactive molecules.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2021.130069DOI Listing
November 2021

Evaluating the genesis and dominant processes of groundwater salinization by using hydrochemistry and multiple isotopes in a mining city.

Environ Pollut 2021 Aug 18;283:117381. Epub 2021 May 18.

School of Resources and Environmental Engineering, Anhui University, Anhui Province Engineering Laboratory for Mine Ecological Remediation, Hefei, 230601, Anhui, China.

The increasing salinization of groundwater renders it challenging to maintain the water quality. Moreover, knowledge regarding the characteristics and mechanism of groundwater salinization in mining areas remains limited. This study represents the first attempt of combining the hydrochemical, isotope (δD, δO, δCl, and Sr/Sr) and multivariate statistical analysis methods to explore the origin, control, and influence of fluoride enrichment in mining cities. The TDS content of groundwater ranged from 275.9 mg/L to 2452.0 mg/L, and 54% of the groundwater samples were classified as class IV water according to China's groundwater quality standards (GB/T 14848-2017), indicating a decline in the water quality of the study area. The results of the groundwater ion ratio and isotope discrimination analysis showed that dissolution and evaporation involving water-rock interactions and halite were the main driving processes for groundwater salinization in the study area. In addition to the hydrogeological and climatic conditions, mine drainage inputs exacerbated the increasing salinity of the groundwater in local areas. The mineral dissolution, cation exchange, and evaporation promoted the F enrichment, while excessive evaporation and salinity inhibited the F enrichment. Gangue accumulation and infiltration likely led to considerable F enrichment in individual groundwater regions. Extensive changes in the groundwater salinity indicated differences in the geochemical processes that controlled the groundwater salinization. Given the particularity of the study area, the enrichment of salinization and fluoride triggered by mining activities cannot be ignored.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2021.117381DOI Listing
August 2021

Study of the micromorphology and health risks of arsenic in copper smelting slag tailings for safe resource utilization.

Ecotoxicol Environ Saf 2021 Aug 12;219:112321. Epub 2021 May 12.

School of Resources and Environmental Engineering, Anhui Province Engineering Laboratory for Mine Ecological Remediation, Anhui University, Hefei 230601, PR China. Electronic address:

Slag tailings are produced by "cooling-grinding-ball milling-flotation" and other processes of slag, while slag is produced by the flash smelting of the original ore. The utilization and environmental hazards of arsenic in slag tailings have become a focus of attention. This study on slag tailings reveals the presence of arsenic in copper smelting tailings from the mineralogy and leaching perspectives, and the noncarcinogenic and carcinogenic risks of arsenic to the human body were assessed by using the USEPA health risk model. The surface particles of the slag tailings were unevenly dispersed, and the mineral crystals were relatively complete. A small amount of secondary minerals had grown on the mineral surface. Most of the fine particles adhered to the surface of the main mineral to form inclusions. The mineral composition of the slag tailings was dominated by maghemite (FeO) and fayalite (FeSiO), and the arsenic-bearing minerals were unevenly distributed, where As (Ⅴ) fine particles were embedded in maghemite, amorphous phase and fayalite. There was a large amount of residual arsenic in the slag tailing particles, and the leaching content of arsenic in the toxicity leaching procedure was always lower than the limit of 5 mg/L. The health risk to the exposed population was evaluated by the USEPA health risk model. Since the exposed population in the industrial land is mainly adults, it is determined that the tailings will not cause harm to children's health. In this evaluation, the exposure duration (length of service of the workers) of 30 years, exposure frequency of 314 d/y and body weight of 60 kg (average weight of the workers) were taken as the parameters of three exposure pathways: hand-oral ingestion, respiratory system inhalation and skin contact. Therefore, longer activity time of the workers in the tailing workshop corresponds to a higher HI (hazard index). Although the arsenic in the slag tailings had a certain degree of bioavailability, it was not sufficient to adversely affect human health.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2021.112321DOI Listing
August 2021

Correlation between serum lipid and prognosis of idiopathic sudden sensorineural hearing loss: a prospective cohort study.

Ann Transl Med 2021 Apr;9(8):676

Department of Otorhinolaryngology, The Second Affiliated Hospital of Shanghai University (Wenzhou Central Hospital), Wenzhou, China.

Background: To investigate the correlation between blood lipids and the prognosis of idiopathic sudden sensorineural hearing loss (ISSNHL).

Methods: We included 232 patients with ISSNHL at the Second Affiliated Hospital of Shanghai University from June 2015 to March 2017 using a prospective cohort study design. We collected information including age, gender, hypertension, diabetes, mellitus, vertigo, as well as the levels of blood total cholesterol (TC), triglycerides (TG), and low-density lipoproteins (LDL-C). We also recorded the ratio between the levels of low-density lipoproteins and the levels of high-density lipoproteins (LDL-C/HDL-C ratio). Correlations between the prognosis of ISSNHL and TC, TG, LDL-C, and LDL-C/HDL-C ratio were analyzed by univariable and multivariable logistic regression analyses.

Results: The clinical effectiveness rate of patients with TC ranging from 5.2 to 6.2 mmol/L was significantly higher than that of patients with TC <5.2 mmol/L (P<0.001). No notable difference was found between patients with TC <5.2 mmol/L and patients with TC ≥6.2 mmol/L. The clinical effectiveness rate of patients in TG ranging from 1.7 mmol/L to 2.3 mmol/L was markedly higher than those in TG <1.7 mmol/L (P<0.001). No significant difference was found between patients with TG ranging from 2.3 to 5.6 mmol/L, TG ≥5.6 mmol/L, and TG <1.7 mmol/L. The clinical effectiveness rate of patients in LDL-C/HDL-C <1.5 was considerably higher than those in LDL-C/HDL-C ranging from 1.5 to 2.5, 2.5 to 3.5, and ≥3.5 (P<0.001).

Conclusions: Our findings indicated that TC, TG, and the LDL-C/HDL-C ratio are strongly associated with the prognosis of ISSNHL. These three indices could be recommended as independent markers to predict outcomes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-21-907DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106097PMC
April 2021

Suspecting unwitnessed hypoglycaemia.

Pract Neurol 2021 Apr 13. Epub 2021 Apr 13.

Department of Neurology and Stroke Medicine, St George's Hospital Atkinson Morley Regional Neuroscience Centre, London, Greater London, UK.

Hypoglycaemic coma can present with acute neurological dysfunction mimicking stroke. It may be masked by normoglycaemia due to physiological compensatory mechanisms (Somogyi phenomenon). Specific MR brain scan changes should alert the clinician to consider this alternative to stroke in the acute setting in patients with a history of recurrent hypoglycaemia, even when the blood glucose is not low at presentation. Rapid identification and treatment of hypoglycaemia may confer a good prognosis with radiological resolution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1136/practneurol-2020-002887DOI Listing
April 2021

Sucrose triggers a novel signaling cascade promoting Bacillus subtilis rhizosphere colonization.

ISME J 2021 09 26;15(9):2723-2737. Epub 2021 Mar 26.

Department of Biology, Northeastern University, Boston, MA, USA.

Beneficial rhizobacteria promote plant growth and protect plants against phytopathogens. Effective colonization on plant roots is critical for the rhizobacteria to exert beneficial activities. How bacteria migrate swiftly in the soil of semisolid or solid nature remains unclear. Here we report that sucrose, a disaccharide ubiquitously deployed by photosynthetic plants for fixed carbon transport and storage, and abundantly secreted from plant roots, promotes solid surface motility (SSM) and root colonization by Bacillus subtilis through a previously uncharacterized mechanism. Sucrose induces robust SSM by triggering a signaling cascade, first through extracellular synthesis of polymeric levan, which in turn stimulates strong production of surfactin and hyper-flagellation of the cells. B. subtilis poorly colonizes the roots of Arabidopsis thaliana mutants deficient in root-exudation of sucrose, while exogenously added sucrose selectively shapes the rhizomicrobiome associated with the tomato plant roots, promoting specifically bacilli and pseudomonad. We propose that sucrose activates a signaling cascade to trigger SSM and promote rhizosphere colonization by B. subtilis. Our findings also suggest a practicable approach to boost prevalence of beneficial Bacillus species in plant protection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41396-021-00966-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397739PMC
September 2021

Healable, Degradable, and Conductive MXene Nanocomposite Hydrogel for Multifunctional Epidermal Sensors.

ACS Nano 2021 04 26;15(4):7765-7773. Epub 2021 Mar 26.

Interdisciplinary Research Center for Artificial Intelligence, College of Materials Science and Engineering, State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China.

Conductive hydrogels have emerged as promising material candidates for epidermal sensors due to their similarity to biological tissues, good wearability, and high accuracy of information acquisition. However, it is difficult to simultaneously achieve conductive hydrogel-based epidermal sensors with reliable healability for long-term usage, robust mechanical property, environmental degradability for decreased electronic waste, and sensing capability of the physiological stimuli and the electrophysiological signals. Herein, we propose the synthesis strategy of a multifunctional epidermal sensor based on the highly stretchable, self-healing, degradable, and biocompatible nanocomposite hydrogel, which is fabricated from the conformal coating of a MXene (TiCT) network by the hydrogel polymer networks involving poly(acrylic acid) and amorphous calcium carbonate. The epidermal sensor can be employed to sensitively detect human motions with the fast response time (20 ms) and to serve as electronic skins for wirelessly monitoring the electrophysiological signals (such as the electromyogram and electrocardiogram signals). Meanwhile, the multifunctional epidermal sensor could be degraded in phosphate buffered saline solution, which could not cause any pollution to the environment. This line of research work sheds light on the fabrication of the healable, degradable, and electrophysiological signal-sensitive conductive hydrogel-based epidermal sensors with potential applications in human-machine interactions, healthy diagnosis, and smart robot prosthesis devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.1c01751DOI Listing
April 2021

Optimizing the heterogeneous network structure to achieve polymer nanocomposites with excellent mechanical properties.

Phys Chem Chem Phys 2021 Feb;23(7):4437-4452

Key Laboratory of Beijing City on Preparation and Processing of Novel Polymer Materials, Beijing University of Chemical Technology, People's Republic of China. and Beijing Engineering Research Center of Advanced Elastomers, Beijing University of Chemical Technology, People's Republic of China and State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, People's Republic of China.

Designing and optimizing the polymer network structure at the molecular level to manipulate its mechanical properties are of great scientific significance. Although heterogeneous multi-network structures have been extensively investigated, little effort has been devoted to investigating heterogeneous single-networks with a well-defined interface. Herein, through coarse-grained molecular dynamics simulation, we successfully fabricated a heterogeneous single-network, which was divided into several regions with different crosslink densities. Firstly, we found that there is an optimal crosslink density ratio between high and low crosslink density regions to obtain the best stress-strain behavior. Secondly, the effect of the regularity of the network topology (by changing the distribution of two-phase regions) on mechanical properties was also studied. It was clearly observed that the polymer network showed better elastic response and mechanical properties as the distribution of two-phase regions became uniform. Finally, we investigated the effect of the selective distribution of nanoparticles (NPs) on mechanical properties by introducing NPs into a pre-designed multiphase network. Results showed that the selective distribution of NPs in the high crosslink density region had a more significant effect on the mechanical reinforcement. Generally, our simulated results may provide some guidelines to design polymer network structures to achieve high-performance polymer nanocomposites with excellent mechanical properties.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0cp06532cDOI Listing
February 2021
-->