Publications by authors named "Liping Bai"

63 Publications

Comparison of blood pressure and pain rating index used for depth regulation of sevoflurane anaesthesia.

J Pak Med Assoc 2021 Feb;71(2(B)):590-595

Department of Anesthesiology, Beijing Sanbo Brian Hospital, Capital Medical University, Beijing, China.

Objectives: The Pain Rating index (PRi) is a new parameter for regulating analgesic depth of anaesthesia based on wavelet analysis. The aim of this study was to investigate the feasibility of PRi for depth regulation of sevoflurane anaesthesia.

Methods: We conducted a monocentric randomized controlled study from September 2017 to June 2018 in patients undergoing anterior cervical discectomy and fusion (ACDF) (n=44). Patients were randomly allocated into two groups and assigned 22 cases to each group: systolic blood pressure group (SBP group) and pain rating index group (PRi group). In SBP group, sevoflurane inhalation concentration (Cs) was adjusted to maintain SBP values at baseline values -20%~+20%; in PRi group, Cs was adjusted to maintain PRi values between 50 and 70. The primary endpoint was anaesthesia recovery time. Secondary endpoints included extubation time, sevoflurane consumption, number of intraoperative haemodynamic instability events /interventions, number of adverse events and postoperative visual analogue scale for pain.

Results: Patient demographic characteristics, surgical time and anaesthesia time did not differ between groups. Anaesthesia recovery time was shorter in PRi group than in SBP group (17.5±3.8min vs 21.5±2.8 min; P=0.001). Extubation time was also shorter in PRi group than in SBP group (21.9±1.7min vs 24.1±2.5min; P=0.001). Sevoflurane consumption was lower in PRi group than in SBP group (15.5±4.1ml vs 20.0±2.5ml; P=0.001).

Conclusions: PRi was feasible to regulate depth of sevoflurane anaesthesia, which could shorten anaesthesia recovery time and extubation time, reduce sevoflurane consumption during general anaesthesia in patients undergoing cervical vertebra surgery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.47391/JPMA.1155DOI Listing
February 2021

Thioredoxin-1 Rescues MPP/MPTP-Induced Ferroptosis by Increasing Glutathione Peroxidase 4.

Mol Neurobiol 2021 Feb 26. Epub 2021 Feb 26.

Laboratory of Molecular Neurobiology, Medical School, Kunming University of Science and Technology, No.727 Jingming South Road, Kunming, 650500, China.

Parkinson's disease (PD), a common neurodegenerative disease, is typically associated with the loss of dopaminergic neuron in the substantia nigra pars compacta (SNpc). Ferroptosis is a newly identified cell death, which associated with iron accumulation, glutathione (GSH) depletion, lipid peroxidation formation, reactive oxygen species (ROS) accumulation, and glutathione peroxidase 4 (GPX4) reduction. It has been reported that ferroptosis is linked with PD.Thioredoxin-1 (Trx-1) is a redox regulating protein and plays various roles in regulating the activity of transcription factors and inhibiting apoptosis. However, whether Trx-1 plays the role in regulating ferroptosis involved in PD is still unknown. Our present study showed that 1-methyl-4-phenylpyridinium (MPP) decreased cell viability, GPX4, and Trx-1, which were reversed by Ferrostatin-1 (Fer-1) in PC 12 cells and SH-SY5Y cells. Moreover, the decreased GPX4 and GSH, and increased ROS were inhibited by Fer-1 and Trx-1 overexpression. We further repeated that behavior deficits resulted from 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) were improved in Trx-1 overexpression transgenic mice. Trx-1 reversed the decreases of GPX4 and tyrosine hydroxylase (TH) induced by MPTP in the substantia nigra pars compacta (SNpc). Our results suggest that Trx-1 inhibits ferroptosis in PD through regulating GPX4 and GSH.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12035-021-02320-1DOI Listing
February 2021

ACBP suppresses the proliferation, migration, and invasion of colorectal cancer via targeting Wnt/beta-catenin signaling pathway.

Biomed Pharmacother 2021 May 10;137:111209. Epub 2021 Feb 10.

Clinical Medical Research Center of the Affiliated Hospital, Inner Mongolia Medical University, Inner Mongolia Key Laboratory of Medical Cell Biology, 1 Tong Dao Street, Hohhot 010050, Inner Mongolia, China. Electronic address:

Anticancer bioactive peptide (ACBP), a novel bioactive peptide isolated from spleens of goats immunized with tumor extracts in our lab, can inhibit the proliferation of CRC in vitro and vivo. However, it remains unclear how the proliferation of CRC is inhibited by ACBP at the molecular level. Here, we provide evidences showing that ACBP significantly inhibits the expression of Wnt/β-catenin related genes (cyclin D1, met and c-myc) through pharmacotranscriptomic and qRT-PCR analysis in CRCs. Active β-catenin, a key protein within Wnt pathway, was compromised remarkably by ACBP in three CRCs, including HCT116, RKO and HT29. Thus nuclear accumulation of active β-catenin was retarded and finally lead to the decreased expression of oncogenes cyclin D1, met, and c-myc. In addition, we proved that active β-catenin reduction was mainly due to the inhibition of phospho-LRP6 and stimulation of phospho-β-catenin by ACBP. Based on the detection of Met and C-Myc in CRC tumor tissue without prior radiotherapy or chemotherapy, our results demonstrated that ACBP can act as a promising anticancer agent for CRC by targeting Wnt/β-catenin pathway, especially active β-catenin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2020.111209DOI Listing
May 2021

Similarity and Specificity of Traditional Chinese Medicine Formulas for Management of Coronavirus Disease 2019 and Rheumatoid Arthritis.

ACS Omega 2020 Dec 18;5(47):30519-30530. Epub 2020 Nov 18.

State Key Laboratory of Quality Research in Chinese Medicines and Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa 999078, Macau, China.

The pathogenesis similarity is leading to the introduction of drugs commonly used in rheumatoid arthritis (RA) into coronavirus disease (COVID-19) treatment. Traditional Chinese medicine (TCM) was widely used for the treatment of infectious diseases and rheumatic diseases. However, there is little knowledge of the relationship between COVID-19 and RA treatment employing TCM formulas. The present work was aimed to compare the similarity and specificity of TCM formulas for the management of COVID-19 and RA, as well as to deduce the potential mechanism of TCM for COVID-19 treatment. Two formulas including lianhuaqingwen (LHQW) and duhuojisheng (DHJS) were selected as the representatives of TCM for COVID-19 and RA treatment, respectively. An integrated network pharmacology was used to investigate their similarity and specificity. Although different herbs are present in the two formulas, they generated fairly similar ingredients, targets, interaction networks and enriched pathways, which were mainly involved in virus infection, inflammation, and immune dysregulation. Undoubtedly, they also exhibited their respective specificity. LHQW showed the cold property and lung channel tropism which dominated heat-clearing and lung-freeing, while DHJS showed the warm property and liver channel tropism. Herbal compatibility of LHQW was more in line with the rules of the TCM formula against coronavirus disease. Although both formulas suggested multifunctionality in virus infection and inflammation, LHQW was inclined to cope with virus infection, while DHJS was inclined to cope with inflammation. Therefore, LHQW was reliable for providing the desired efficacy in COVID-19 management because of its cold property, lung channel tropism, and multifunctionality for coping with virus infection and inflammation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.0c04377DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711705PMC
December 2020

Defensive functions of volatile organic compounds and essential oils from northern white-cedar in China.

BMC Plant Biol 2020 Nov 3;20(1):500. Epub 2020 Nov 3.

College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, Liaoning Province, China.

Background: Plants are known to emit diverse volatile organic compounds (VOCs), which may function as signaling substances in plant communication with other organisms. Thuja occidentalis, which is widely cultivated throughout China, releases aromatic VOCs into the air in winter and early spring. The relationship of this cultivated plant with its neighboring plants is necessary for the conservation of biodiversity.

Results: (-)-α-thujone (60.34 ± 5.58%) was found to be the major component in VOCs from the Shenyang population. The essential oils (EOs) from the Kunming and Shenyang populations included the major components (-)-α-thujone, fenchone, (+)-β-thujone, and (+)-hibaene, identified using GC-MS analyses. (-)-α-thujone and (+)-hibaene were purified and identified by NMR identification. EOs and (-)-α-thujone exhibited valuable phytotoxic activities against seed germination and seedling growth of the plants Taraxacum mongolicum and Arabidopsis thaliana. Moreover, the EOs displayed potent inhibitory activity against pathogenic fungi of maize, including Fusarium graminearum, Curvularia lunata, and Bipolaris maydis, as well as one human fungal pathogen, Candida albicans. Quantitative analyses revealed high concentrations of (-)-α-thujone in the leaves of T. occidentalis individuals from both the Shenyang and Kunming populations. However, (-)-α-thujone (0.18 ± 0.17 μg/g) was only detected in the rhizosphere soil to a distance of 0.5 m from the plant.

Conclusions: Taken together, our results suggest that the phytotoxic effects and antifungal activities of the EOs and (-)-α-thujone in T. occidentalis certainly increased the adaptability of this plant to the environment. Nevertheless, low concentrations of released (-)-α-thujone indicated that reasonable distance of T. occidentalis with other plant species will impair the effects of allelochemical of T. occidentalis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12870-020-02716-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607654PMC
November 2020

MUC1-induced immunosuppression in colon cancer can be reversed by blocking the PD1/PDL1 signaling pathway.

Oncol Lett 2020 Dec 1;20(6):317. Epub 2020 Oct 1.

Department of Gastrointestinal Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China.

Mucin1 (MUC1) upregulation in colon cancer has been linked to poor patient outcomes and advanced stage at diagnosis. This is partially due to MUC1-mediated inhibition of T-cell proliferation affecting efficient lysis by cytotoxic lymphocytes, which contributes to escape from immune surveillance. In the present study, human colorectal cancer tissues were collected, and MUC1-positive and MUC1-negative colon cancer mouse models were prepared; subsequently, the number and function of immune cells in tumor tissues were measured using flow cytometry. The present study revealed that MUC1, as a tumor-associated antigen, can recruit more tumor-infiltrating lymphocytes into the tumor microenvironment compared with MUC1-negative colon cancer, but that these cells could not serve antitumor roles. Conversely, the present study demonstrated that MUC1-positive colon cancer attracted more regulatory T cells (Treg cells), myeloid-derived suppressor cells (MDSCs) and tumor-associated macrophages (TAMs) to the tumor site than MUC1-negative colon cancer. Furthermore, the data suggested that programmed death protein 1 (PD1)-programmed death ligand 1 (PDL1) expression is greater in MUC1-positive colon cancer. Blocking the PD1-PDL1 signaling pathway reduced the percentage of Treg cells, MDSCs and TAMs in the tumor microenvironment, enhanced T-cell cytotoxicity and inhibited tumor growth, prolonging the survival time of MUC1-positive tumor-bearing mice. Therefore, the present study elucidated the role of MUC1 in tumor immune escape and provides a foundation for the application of PDL1 inhibitors to MUC1-positive colon cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/ol.2020.12180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590440PMC
December 2020

TRPV4 contributes to ER stress: Relation to apoptosis in the MPP-induced cell model of Parkinson's disease.

Life Sci 2020 Nov 19;261:118461. Epub 2020 Sep 19.

Medical School, Kunming University of Science and Technology, Kunming 650500, China. Electronic address:

Aims: Parkinson's disease (PD) is a multifactorial neurodegenerative disorder. Its molecular mechanism is still unclear. Endoplasmic reticulum (ER) stress has been highlighted in PD. Transient receptor potential vanilloid 4 (TRPV4) is a kind of nonselective calcium cation channel. A defined role for TRPV4 in PD has not been reported. The purpose of the present research was to investigate the molecular mechanisms by which TRPV4 regulates ER stress induced by the 1-methyl-4-phenylpyridinium ion (MPP) in PC12 cells.

Main Methods: PC12 cells were pretreated with the TRPV4-specific antagonist HC067047 or transfected with TRPV4 siRNA followed by treatment with MPP. Cell viability was measured by the CCK-8 Assay. The expression of TRPV4, sarco/endoplasmic reticulum Ca-ATPase 2 (SERCA2), glucose-regulated protein 78 (GRP78), glucose-regulated protein 94 (GRP94), C/EBP homologous protein (CHOP), procaspase-12, and tyrosine hydroxylase (TH) was detected by western blot and RT-PCR.

Key Findings: The expression of TRPV4 was upregulated, while cell viability was decreased by MPP, which was reversed by HC067047. The ER stress common molecular signature SERCA2 was depressed by MPP. Moreover, MPP induced upregulation of GRP78, GRP94, CHOP, and decrease in procaspase-12 and TH. HC067047 and TRPV4 siRNA reversed MPP-induced ER stress and restored TH production.

Significance: TRPV4 functions upstream of ER stress induced by MPP and holds promise as a prospective pharmacotherapy target for PD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2020.118461DOI Listing
November 2020

Validation of PREdiction of DELIRium in ICu patients (PRE-DELIRIC) among patients in intensive care units: A retrospective cohort study.

Nurs Crit Care 2020 Sep 20. Epub 2020 Sep 20.

The Nethersole School of Nursing, The Chinese University of Hong Kong, Shatin, Hong Kong SAR.

Background: An intensive care unit (ICU) delirium prediction tool, PREdiction of DELIRium in ICu patients (PRE-DELIRIC), has been developed and calibrated in a multinational project. However, there is a lack of evidence regarding the predictive ability of the PRE-DELIRIC among Chinese ICU patients.

Aims And Objectives: To evaluate the predictive validity (discrimination and calibration) of PRE-DELIRIC.

Methods: A retrospective cohort study was conducted. Consecutive participants (a) admitted to the ICU for ≥24 hours, (b) aged ≥18 years, and (c) admitted to the ICU for the first time were included. Ten predictors (age, APACHE-II, urgent and admission category, urea level, metabolic acidosis, infection, coma, sedation, and morphine use) assessed within 24 hours upon ICU admission were assessed. Delirium was assessed using the Confusion Assessment Method for ICU. Outcomes included ICU length of stay and mortality. Discrimination and calibration were determined by the areas under the receiver operating characteristic curve (AUROC), box plot, and calibration plot.

Results: A total of 375 ICU patients were included, with 44.0% of patients being delirious. Delirium was significantly associated with age, PRE-DELIRIC score, ICU length of stay, and mortality. The AUROC was 0.81 (95% confidence interval, 0.77-0.86). The optimal cut-off point identified by max Youden index was 49%. The calibration plot of pooled data demonstrated a calibration slope of 0.894 and an intercept of -0.178.

Design: This is a retrospective cohort study.

Conclusions: The PRE-DELIRIC has high predictive value and is suggested to be adopted in ICUs for early initiation of preventive interventions against delirium among high-risk patients.

Relevance To Clinical Practice: Clinicians can adopt the PRE-DELIRIC among ICU patients to screen patients at high risk of developing delirium. Early initiative interventions could be implemented to reduce the negative impacts of ICU delirium.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/nicc.12550DOI Listing
September 2020

Targeting Biofilms Therapy: Current Research Strategies and Development Hurdles.

Microorganisms 2020 Aug 11;8(8). Epub 2020 Aug 11.

NHC Key Laboratory of Biotechnology of Antibiotics, CAMS Key Laboratory of Synthetic Biology for Drug Innovation, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.

Biofilms are aggregate of microorganisms in which cells are frequently embedded within a self-produced matrix of extracellular polymeric substance (EPS) and adhere to each other and/or to a surface. The development of biofilm affords pathogens significantly increased tolerances to antibiotics and antimicrobials. Up to 80% of human bacterial infections are biofilm-associated. Dispersal of biofilms can turn microbial cells into their more vulnerable planktonic phenotype and improve the therapeutic effect of antimicrobials. In this review, we focus on multiple therapeutic strategies that are currently being developed to target important structural and functional characteristics and drug resistance mechanisms of biofilms. We thoroughly discuss the current biofilm targeting strategies from four major aspects-targeting EPS, dispersal molecules, targeting quorum sensing, and targeting dormant cells. We explain each aspect with examples and discuss the main hurdles in the development of biofilm dispersal agents in order to provide a rationale for multi-targeted therapy strategies that target the complicated biofilms. Biofilm dispersal is a promising research direction to treat biofilm-associated infections in the future, and more in vivo experiments should be performed to ensure the efficacy of these therapeutic agents before being used in clinic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/microorganisms8081222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7465149PMC
August 2020

20()-Protopanaxatriol promotes the binding of P53 and DNA to regulate the antitumor network multiomic analysis.

Acta Pharm Sin B 2020 Jun 5;10(6):1020-1035. Epub 2020 Feb 5.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300353, China.

Although the tumor suppressor P53 is known to regulate a broad network of signaling pathways, it is still unclear how certain drugs influence these P53 signaling networks. Here, we used a comprehensive single-cell multiomics view of the effects of ginsenosides on cancer cells. Transcriptome and proteome profiling revealed that the antitumor activity of ginsenosides is closely associated with P53 protein. A miRNA-proteome interaction network revealed that P53 controlled the transcription of at least 38 proteins, and proteome-metabolome profiling analysis revealed that P53 regulated proteins involved in nucleotide metabolism, amino acid metabolism and "Warburg effect". The results of integrative multiomics analysis revealed P53 protein as a potential key target that influences the anti-tumor activity of ginsenosides. Furthermore, by applying affinity mass spectrometry (MS) screening and surface plasmon resonance fragment library screening, we confirmed that 20()-protopanaxatriol directly targeted adjacent regions of the P53 DNA-binding pocket and promoted the stability of P53-DNA interactions, which further induced a series of omics changes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.apsb.2020.01.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7332671PMC
June 2020

Trichomicin Suppresses Colorectal Cancer Comprehensive Regulation of IL-6 and TNFα in Tumor Cells, TAMs, and CAFs.

Front Pharmacol 2020 3;11:386. Epub 2020 Apr 3.

NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Trichomicin, a small-molecule compound isolated from fungi, has been identified with bioactivity of antitumor. In this study, a colon cancer subcutaneous mice model was used to evaluate the antitumor effects of Trichomicin . Treatment with Trichomicin significantly inhibited tumor growth in a xenograft mouse colon cancer model. The underlying molecular mechanism has also been investigated through the quantification of relevant proteins. The expression levels of IL-6 and TNFα were reduced in tumor tissues of mice treated with Trichomicin, which was consistent with results of experiments in which Trichomicin suppressed the expression of IL-6 and TNFα in tumor and stromal cells. In addition, Trichomicin inhibited TNFα-induced activation of NF-κB and basal Stat3 signaling , which resulted in reduced expression of the immune checkpoint protein PD-L1 in tumor and stromal cells. Conclusively, Trichomicin, a promising new drug candidate with antitumor activity, exerted antitumor effects against colon cancer through inhibition of the IL-6 and TNFα signaling pathways.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2020.00386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146085PMC
April 2020

Thioredoxin-1 blocks methamphetamine-induced injury in brain through inhibiting endoplasmic reticulum and mitochondria-mediated apoptosis in mice.

Neurotoxicology 2020 05 20;78:163-169. Epub 2020 Mar 20.

Medical School, Kunming University of Science and Technology, Kunming, 650500, China. Electronic address:

Methamphetamine (METH) has been reported to induce endoplasmic reticulum (ER) stress and neuronal apoptosis in the central nervous system (CNS) during the development of addiction. Thioredoxin-1 (Trx-1) is a redox regulating protein and plays an important role in inhibiting apoptosis and protects neurons from cytotoxicity through ER and mitochondria-mediated pathways. Our previous study has been reported that Trx-1 protects mice from METH-induced rewarding effect. However, whether Trx-1 plays the role in resisting METH injury is still unclear. Here, we aim to investigate whether Trx-1 participates in the regulation of METH-induced CNS injury via ER stress and mitochondria-mediated pathways. Our study first repeated the conditioned place preference expression induced by METH. Then we detected and found that METH increased the expression of N-methyl-d-asparate (NMDA) receptor subunit 2B (NR2B) and the level of glutamate (Glu) in the ventral tegmental area (VTA) and nucleus accumbens (NAc), while Trx-1 overexpression suppressed the increases. We further examined ER stress-related proteins and mitochondrial apoptosis pathway in the VTA and NAc, and found that METH increased the expressions of glucose regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), and Bax, as same time decreased the expressions of procaspase12, Bcl-2, and procaspase3, while Trx-1 overexpression blocked these changes. These results indicate that Trx-1 blocks METH-induced injury by suppressing ER stress and mitochondria-mediated apoptosis in the VTA and NAc via targeting glutamatergic system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neuro.2020.03.006DOI Listing
May 2020

A novel S1P1 modulator IMMH002 ameliorates psoriasis in multiple animal models.

Acta Pharm Sin B 2020 Feb 14;10(2):276-288. Epub 2019 Nov 14.

Department of Thoracic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.

Psoriasis is characterized by abnormal proliferation of keratinocytes, as well as infiltration of immune cells into the dermis and epidermis, causing itchy, scaly and erythematous plaques of skin. The understanding of this chronic inflammatory skin disease remains unclear and all available treatments have their limitations currently. Here, we showed that IMMH002, a novel orally active S1P modulator, desensitized peripheral pathogenic lymphocytes to egress signal from secondary lymphoid organs and thymus. Using different psoriasis animal models, we demonstrated that IMMH002 could significantly relieve skin damage as revealed by PASI score and pathological injure evaluation. Mechanistically, IMMH002 regulated CD3 T lymphocytes re-distribution by inducing lymphocytes' homing, thus decreased T lymphocytes allocation in the peripheral blood and skin but increased in the thymus. Our results suggest that the novel S1P agonist, IMMH002, exert extraordinary capacity to rapidly modulate T lymphocytes distribution, representing a promising drug candidate for psoriasis treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.apsb.2019.11.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016294PMC
February 2020

Complete Genome Sequence of the Ebosin-Producing Strain sp. 139.

Microbiol Resour Announc 2019 Dec 5;8(49). Epub 2019 Dec 5.

NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

Members of the genus are known for their ability to produce compounds with various bioactivities and for their complex morphologies. sp. strain 139 is the producer strain of the exopolysaccharide (EPS) ebosin, which has remarkable antirheumatic arthritis activity. Here, we report its complete genome sequence, which will facilitate the study of the biosynthesis of ebosin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1128/MRA.01283-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895308PMC
December 2019

A new compound Trichomicin exerts antitumor activity through STAT3 signaling inhibition.

Biomed Pharmacother 2020 Jan 7;121:109608. Epub 2019 Nov 7.

NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China; Department of Surgery and Ellis Fischel Cancer Center, University of Missouri-Columbia, Columbia, MO, 65212, United States. Electronic address:

Trichomicin, a novel small-molecule compound isolated from the fungus Trichoderma harzianum and identified as new structure compound, exhibited antitumor activities in various human cancer cell lines and reversed drug resistance activity in the multidrug-resistant cancer cell line KBV. The underlying cellular and molecular mechanism was illuminated. Trichomicin can significantly induce cancer cell apoptosis and reduced IL-6 expression and phosphorylation of STAT3 were found in response to Trichomicin treatment. The blockade of IL-6 mediated JAK-STAT3 signaling pathway by Trichomicin was confirmed using reporter gene system. As a promising antitumor-activity compound, Trichomicin is presented in this study.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2019.109608DOI Listing
January 2020

MicroRNA-31 is a potential biomarker for screening B-lymphoblastic leukemia in children.

Oncol Lett 2019 Nov 10;18(5):4930-4935. Epub 2019 Sep 10.

Department of Clinical Laboratory, Hongqi Hospital Affiliated to Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China.

The present study aimed to investigate the expression and significance of microRNA-31 (miR-31) in children with acute B-lymphoblastic leukemia (B-ALL). Bone marrow specimens and peripheral blood were collected from children with B-ALL (n=38) and healthy controls (n=18). Total RNA was extracted and the expression levels of miR-31 were measured using quantitative PCR. In addition, a receiver operating characteristic curve was generated, and the area under the curve (AUC) was calculated to evaluate the diagnostic value of miR-31 for the development of B-ALL. miR-31 expression was significantly lower in the B-ALL group compared with in the control group (P<0.05). Additionally, the expression levels of miR-31 in the B-ALL group before treatment were markedly lower than in the B-ALL group after treatment, and miR-31 expression was significantly lower after 30 days of treatment compared with after 12 weeks of treatment. Furthermore, miR-31 expression in the group of children ≥10 years of age was higher than that in the group of children <10 years of age. Furthermore, the expression levels of miR-31 were higher in the low-risk group compared with in the medium- and high-risk groups (P<0.05). When the cutoff value was set at 1.8, the AUC of miR-31 for B-ALL diagnosis was 0.915 (95% CI, 0.828-1.000; P<0.0001), with a sensitivity and specificity of 80.8 and 100%, respectively. In conclusion, miR-31 may exert an anticancer role in B-ALL in children and may be a potential marker to assist in diagnosis and prognostic prediction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/ol.2019.10843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6781673PMC
November 2019

Specific PCR method for detection of species origin in biochemical drugs via primers for the ATPase 8 gene by electrophoresis.

Mikrochim Acta 2019 08 19;186(9):634. Epub 2019 Aug 19.

NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China.

A PCR method is described to identify the species origin of various animal and human tissue-derived biochemical drugs. Four commercialized drugs, including spermary tablets, compound embryonic bovine liver extract tablets, spleen aminopeptide solution, and placenta polypeptide injection, were used as a proof-of-principle in this study. Primers were designed to amplify conservative regions of mitochondrial cytochrome b and ATPase 8 genes from beef, pork, lamb and human DNA, respectively. The specificity of primers for ATPase 8 gene is found to be higher than those for cytochrome b under the given experimental conditions. The amplicon sizes of ATPase 8 were 212, 271, 293 and 405 bp for pork, beef, lamb and human tissue, respectively. The minimum detectable concentration of DNA sample for species identification is 0.05-0.5 pg·μL. The species origin can be distinguished by this method in extremely low concentrations of template DNAs extracted. Conceivably, this PCR method for meat authentication may be extended to quality control of other biochemical drugs and raw materials. Graphical abstract A specific PCR method was developed for the detection of species origin in biochemical drugs via species-specific primers targeting mitochondrial ATPase 8 genes. The PCR products were separated by gel electrophoresis and species origins were indicated by comparison to references.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00604-019-3738-5DOI Listing
August 2019

The gal80 Deletion by CRISPR-Cas9 in Engineered Saccharomyces cerevisiae Produces Artemisinic Acid Without Galactose Induction.

Curr Microbiol 2019 Nov 7;76(11):1313-1319. Epub 2019 Aug 7.

NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Tian Tan Xi Li No. 1, Beijing, 100050, China.

The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas system has emerged as the dominating tool for genome engineering, while also changes the speed and efficiency of metabolic engineering in conventional and non-conventional yeasts. Among these CRISPR-Cas systems, CRISPR-Cas9 technology has usually been applied for removing unfavorable target genes. Here, we used CRISPR-Cas9 technology to delete the gal80 gene in uracil-deficient strain and had successfully remolded the engineered Saccharomyces cerevisiae that can produce artemisinic acid without galactose induction. An L(3) orthogonal test was adopted to investigate the effects of different factors on artemisinic acid production. Fermentation medium III with sucrose as carbon sources, 1% inoculum level, and 84-h culture time were identified as the optimal fermentation conditions. Under this condition, the maximum artemisinic acid production by engineered S. cerevisiae 1211-2 was 740 mg/L in shake-flask cultivation level. This study provided an effective approach to reform metabolic pathway of artemisinic acid-producing strain. The engineered S. cerevisiae 1211-2 may be applied to artemisinic acid production by industrial fermentation in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00284-019-01752-2DOI Listing
November 2019

Absorption, accumulation and distribution of metals and nutrient elements in poplars planted in land amended with composted sewage sludge: A field trial.

Ecotoxicol Environ Saf 2019 Oct 29;182:109360. Epub 2019 Jun 29.

Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Ecology and Environment, State Forestry Administration, Beijing, 100091, China.

Interest in the application of sewage sludge as amendments to grow trees has continued to increase, especially for fast-growing trees such as poplars. In this study, two-year field trial was conducted to determine the effects of compost sewage sludge (CSS) soil application on the distributions of metal and nutrient elements in poplars (Populus × euramericana 'Guariento') and poplar growth. Soil was amended with one of four CSS treatments in both study years: control (2012, 2013: 0 t/ha), SS1 (2012: 7.5 t/ha, 2013: 15 t/ha), SS2 (2012: 15 t/ha, 2013: 30 t/ha), and SS3 (2012: 30 t/ha, 2013: 45 t/ha). During the two-year field trial period, CSS treatments significantly affected leaf K, Mg, Ni, Cr, and Pb contents and root P contents. The element contents in different plant parts responded differently to the different CSS application rates; microelement contents in roots and trace element contents in leaves were significantly affected by the high sludge treatment. The CSS application significantly influenced Ca, Na, Cu, Ni, and Pb accumulation in aerial parts of poplar and the distributions of N, S, Ni, Mg, and P between roots and leaves or stems, and significantly increased the diameter at breast height (DBH) of poplars by 2.4-18.6%. The CSS application of 15 t/ha per year resulted in the largest average increase in DBH of 11.1%; therefore, it could be considered as the most suitable application rate. In summary, CSS application can improve nutrition uptake in various parts of poplars and promote the growth of poplar. Poplar forest amendment is a good CSS disposal strategy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2019.06.043DOI Listing
October 2019

Genome-Guided Discovery of Pretilactam from ATCC 31565.

Molecules 2019 Jun 19;24(12). Epub 2019 Jun 19.

NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

is a small but well-known genus of actinomycetes for production of ansamitocin, the payload component of antibody-drug conjugates against cancers. However, the secondary metabolite production profile of ATCC 31565, the most famous producer of ansamitocin, has never been fully explored. Our antiSMASH analysis of the genomic DNA of ATCC 31565 revealed a NRPS-PKS gene cluster for polyene macrolactam. The gene cluster is very similar to gene clusters for mirilactam and salinilactam, two 26-membered polyene macrolactams from and , respectively. Guided by this bioinformatics prediction, we characterized a novel 26-membered polyene macrolactam from ATCC 31565 and designated it pretilactam. The structure of pretilactam was elucidated by a comprehensive analysis of HRMS, 1D and 2D-NMR, with absolute configuration of chiral carbons predicted bioinformatically. Pretilactam features a dihydroxy tetrahydropyran moiety, and has a hexaene unit and a diene unit as its polyene system. A preliminary antibacterial assay indicated that pretilactam is inactive against and .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules24122281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631532PMC
June 2019

Determination of epoxide impurity in sarpogrelate hydrochloride intermediate by UHPLC and column-switching liquid chromatography.

J Pharm Biomed Anal 2019 Sep 24;174:57-62. Epub 2019 May 24.

Institute of Medicinal Biotechnology, Peking Union Medical College & Chinese Academy of Medical Sciences, No 1, Tiantan Xili, Beijing 100050, PR China. Electronic address:

The determination of genotoxic impurities, which is closely related to toxicological concern and daily dose, plays a key role in drug quality control. Epoxide impurity is a kind of genotoxic impurity with an epoxy ring structure during the synthesis process of sarpogrelate hydrochloride. According to the sarpogrelate hydrochloride daily dose, epoxide impurity is limited to the under 5 ppm level. The liquid chromatographic-tandem mass spectrometric (LC/MS/MS) or the gas chromatography-mass spectrometric (GC/MS) method is commonly used to characterize the epoxide impurity of sarpogrelate hydrochloride intermediates. However, these methods are not simple or economical enough to detect epoxide impurity. In this study, we resolved the problem by using the most common UV method with two ideas: one was to improve the absolute sensitivity, and the other was to reduce matrix effects. Both ultra high-performance liquid chromatography (UHPLC with high sensitivity LightPipe™ flow cells) and column-switching liquid chromatography methods were developed and validated for the quantitative determination of epoxide impurity in sarpogrelate hydrochloride intermediates. The limits of detection (LODs) of the UHPLC and column-switching liquid chromatography methods were 0.09 ppm (0.09 μg/g) and 0.33 ppm (0.33 μg/g), and the recovery rates of both methods were 87.2%-132.1% and 97.4%-100.1%, respectively. Both methods established and provided guidance for analysts to develop procedures for impurity control, especially for structures of impurity with similar matrices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jpba.2019.05.053DOI Listing
September 2019

A Highly Selective Turn-on and Reversible Fluorescent Chemosensor for Al Detection Based on Novel Salicylidene Schiff Base-Terminated PEG in Pure Aqueous Solution.

Polymers (Basel) 2019 Mar 27;11(4). Epub 2019 Mar 27.

School of Materials Science and Engineering, Liaocheng University, Liaocheng 252059, China.

The development of highly selective and sensitive chemosensors for Al detection in pure aqueous solution is still a significant challenge. In this work, a novel water-soluble polymer PEGBAB based on salicylidene Schiff base has been designed and synthesized as a turn-on fluorescent chemosensor for the detection of Al in 100% aqueous solution. PEGBAB exhibited high sensitivity and selectivity to Al over other competitive metal ions with the detection limit as low as 4.05 × 10 M. PEGBAB displayed high selectivity to Al in the pH range of 5⁻10. The fluorescence response of PEGBAB to Al was reversible in the presence of ethylenediaminetetraacetic acid (EDTA). Based on the fluorescence response, an INHIBIT logic gate was constructed with Al and EDTA as two inputs. Moreover, test strips based on PEGBAB were fabricated facilely for convenient on-site detection of Al.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym11040573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523932PMC
March 2019

JAX2, an ethanol extract of Hyssopus cuspidatus Boriss, can prevent bronchial asthma by inhibiting MAPK/NF-κB inflammatory signaling.

Phytomedicine 2019 Apr 31;57:305-314. Epub 2018 Dec 31.

Xinjiang Institute of Materia Medica, Urumqi 830004, China. Electronic address:

Background: Hyssopus cuspidatus Boriss has been used to treat bronchial asthma for many years in Uighur medicine. JAX2, an ethanol extract from this plant, has effectiveness against bronchial asthma. However, the molecular basis for the anti-inflammatory effects of JAX2 remains unclear.

Purpose: This study aimed to evaluate the mechanism of JAX2 against bronchial asthma.

Methods: We established an asthma model in rats using ovalbumin (OVA), and an inflammatory model in RAW264.7 cells using lipopolysaccharide (LPS) stimulation. To evaluate the anti-inflammatory effects of JAX2, the concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-4, IL-6, IL-17, eotaxin and immunoglobulin (Ig)E were measured by enzyme-linked immunosorbent assay (ELISA). Cell viability was investigated by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)2H-tetrazolium, inner salt (MTS) assay. Further, nitric oxide (NO) and reactive oxygen species (ROS) were determined using Griess reagent and 2,7-dichlorofluorescein diacetate. The phosphorylation of p-extracellular signal-regulated kinase (ERK), p-Jun-NH2-terminal kinase (JNK), p38 kinases (p38) and p-inhibitor of nuclear factor kappa-B kinase (IKK), and nuclear translocation of p-p65 kinases (p-p65) were determined by immunofluorescence to uncover the effects of JAX2 on the Mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathways.

Results: After JAX2 administration to rats, Interferon (IFN)-γ concentrations in BALF increased significantly. Further, the concentrations of TNF-α, IL-4, IL-6, IL-17 and eotaxin in BALF, and IgE in serum decreased. JAX2 decreased TNF-α, IL-6 and NO in cell supernatant, and reduced ROS intracellularly. Concurrently, IFN-γ concentrations increased in cell supernatant significantly. In LPS-induced RAW264.7 cells, JAX2 inhibited phosphorylation of p-ERK, p-JNK and p-38 MAPK. The subsequent phosphorylation of p-IKK and nuclear translocation of the p-p65 subunit of NF-κB were also suppressed.

Conclusion: Based on these findings, we believe that JAX2 has both preventive and treatment effects in bronchial asthma. Furthermore, in the RAW264.7 cell inflammatory model, JAX2 also inhibited NF-κB and MAPK signaling pathways.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.phymed.2018.12.043DOI Listing
April 2019

A simple turn-on fluorescent chemosensor based on Schiff base-terminated water-soluble polymer for selective detection of Al in 100% aqueous solution.

Spectrochim Acta A Mol Biomol Spectrosc 2019 May 19;214:436-444. Epub 2019 Feb 19.

School of Materials Science and Engineering, Liaocheng University, Liaocheng 252059, China. Electronic address:

A simple water-soluble polymer PEGBHB based on polyethylene glycol bearing a Schiff base derivative moiety was successfully designed and synthesized. PEGBHB showed high selectivity and sensitivity towards Al as a turn-on fluorescent chemosensor without influence by other competitive metal ions in 100% aqueous solution. The detection limit of PEGBHB for Al was found to be 9.67 × 10 M. A 1:1 stoichiometry between PEGBHB and Al has been confirmed by Job plot analysis. PEGBHB could detect Al over a wide pH range from 4 to 10. The chemosensor was reversible by adding EDTA to the solution of PEGBHB-Al complex. An INHIBIT molecular logic gate was constructed with the help of OFF-ON-OFF signal on alternate addition of Al and EDTA to the chemosensor. Furthermore, test papers were fabricated facilely using PEGBHB for convenient and visual detection of practical Al.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2019.02.062DOI Listing
May 2019

Brain-derived neurotrophic factor induces thioredoxin-1 expression through TrkB/Akt/CREB pathway in SH-SY5Y cells.

Biochimie 2019 May 20;160:55-60. Epub 2019 Feb 20.

Laboratory of Molecular Neurobiology, Medical Faculty, Kunming University of Science and Technology, Kunming, 650500, China. Electronic address:

Brain-derived neurotrophic factor (BDNF) is one of the neurotrophic factors that are vital to the survival and proliferation of neuron. Thioredoxin-1 (Trx-1) is a redox regulating protein and plays various roles in regulating transcript factors and inhibiting apoptosis. It has been reported that Trx-1 is required for nerve growth factor-mediated signal transduction and neurite outgrowth, and is involved in synaptic protein expression induced by BDNF. However, the molecular mechanism on BDNF inducing Trx-1 expression has not been fully verified. The present study investigated the expression of Trx-1 after treatment with BDNF in SH-SY5Y cells. We first demonstrated that cell viability and the expression of Trx-1 were increased by BDNF in SH-SY5Y cells, which were inhibited by the tyrosine kinase B (TrkB) inhibitor, K252a, and the phosphatidylinositol 3-kinase (PI3-K) inhibitor, LY294002. Moreover, BDNF increased the activity of cAMP response element-binding protein (CREB) through TrkB/PI3-K/Akt pathway. Whereas the expression of Trx-1 induced by BDNF was suppressed by CREB siRNA. Thus, our data suggest that BDNF induces the expression of Trx-1 through the TrkB/Akt/CREB pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2019.02.011DOI Listing
May 2019

Trichosanthin inhibits the proliferation of cervical cancer cells and downregulates STAT-5/C-myc signaling pathway.

Pathol Res Pract 2019 Apr 11;215(4):632-638. Epub 2018 Dec 11.

Department of Gynecology, West China Second Hospital, Sichuan University, No. 20, Third Part of South Renmin Road, Wuhou District, Chengdu, 610041, Sichuan, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, West China Second Hospital, Sichuan University, Chengdu, 610041, China. Electronic address:

Background: Previous studies have indicated that Trichosanthin (TCS) exerts anti-virus, immunoregulation and a broad spectrum anti-tumor pharmacological activities. Trichosanthin is a promising agent for the treatment of cervical cancer. However, the exact effects and potential mechanism of TCS on cervical cancer are not well known.

Method: The cell viability of TCS on cervical cancer cell lines (HeLa and caski cells) were detected by a Cell Counting Kit-8 (CCK-8) assay. Cell proliferation was measured by Ki-67 staining and cell apoptosis was detected by flow cytometry. Cell migration and invasion were detected by wound assay and transwell assay, respectively. The levels of E-cadherin, N-cadherin, Snail, Bcl-2, Caspase-3, p-STAT5, STAT5, p-C-myc, C-myc were detected by western blot.

Results: The present study showed that TCS inhibited the proliferation of HeLa and caski cells and reduced Ki-67 and P-C-myc expression. In addition, flow cytometric analysis showed that TCS induced the apoptosis of HeLa and caski cells. The potent effect of TCS on cell apoptosis as determined by the increase the levels of caspase-3 and decrease the levels of Bcl-2. TCS also inhibited cervical cancer cell invasion, migration and epithelial-mesenchymal transition (EMT). Furthermore, TCS treatment markedly inhibited the activation of STAT5/C-myc signaling pathway.

Conclusion: In conclusion, the present study suggest that TCS inhibits the proliferation, migration and EMT of human cervical cancer cells, which maybe mediated by inhibiting the activation of STAT5/C-myc signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.prp.2018.12.010DOI Listing
April 2019

HDAC6 inhibition induces the failure of mouse early embryonic development.

J Cell Physiol 2019 06 28;234(6):8752-8759. Epub 2018 Oct 28.

NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Histone deacetylases (HDACs) have been implicated in numerous biological events. However, to date, the role of HDAC6 in early embryos remains unknown. In the current study, Tubastatin A (TubA), a potent HDAC6 inhibitor, was used to block HDAC6 activity in mouse embryos. We found that TubA exposure significantly reduced the blastocyst formation of early embryos. Confocal microscopy revealed the markedly increased chromosomal congression failure in the mouse embryos treated with the HDAC6 inhibitor. Moreover, the HDAC6 inhibition resulted in the overproduction of reactive oxygen species (ROS) in embryos. In addition, we observed the accumulation of phosphorylated γH2AX in TubA-treated embryos, indicative of the increased DNA damage. In line with this, cell apoptosis of blastocysts was frequently detected in HDAC6-deficient embryos compared with their controls. Altogether, our data indicate that HDAC6 may serve as an important regulator of chromatin structure and mitochondrial function, determining the developmental potential of the early embryos of mouse.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcp.27534DOI Listing
June 2019

A dual chemosensor for Cu and Hg based on a rhodamine-terminated water-soluble polymer in 100% aqueous solution.

Analyst 2018 Nov;143(22):5395-5403

School of Materials Science and Engineering, Liaocheng University, Liaocheng 252059, China.

A novel water-soluble polymer bearing a rhodamine receptor (PEGSRh) was synthesized as a dual chemosensor for the recognition of Cu2+ and Hg2+ in 100% aqueous solution. PEGSRh not only exhibited a sensitive colorimetric response towards Cu2+ and Hg2+ but also showed a selective turn-on fluorescence response towards Hg2+ over other metal ions. The binding stoichiometry for the complexation of PEGSRh with Cu2+ and Hg2+ was confirmed to be 1 : 1 by Job plot analysis. The low detection limits were found to be 5.92 × 10-7 M for Cu2+ and 2.85 × 10-6 M for Hg2+. The responses of PEGSRh to Cu2+ and Hg2+ were both stable over wide pH ranges. In addition, the fluorescence intensity changes of PEGSRh solution by the inputs of Hg2+, Cu2+ and EDTA have been used to construct a combinational logic gate. Again, an INHIBIT logic gate was also obtained by employing Cu2+ and EDTA as the chemical inputs and the absorbance signal as the output. Moreover, test papers were prepared facilely using PEGSRh for practical on-site detection of Cu2+ and Hg2+.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8an01130cDOI Listing
November 2018

Fast simultaneous determination of main components and impurity sodium ion in PAMA injection by mixed-mode chromatography.

J Pharm Biomed Anal 2018 Nov 3;161:407-413. Epub 2018 Sep 3.

Institute of Medicinal Biotechnology, Peking Union Medical College & Chinese Academy of Medical Sciences, 100050, Tian Tan Xi Li No.1,Beijing, China. Electronic address:

This study describes an analytical method to control the quality of potassium aspartate and magnesium aspartate (PAMA) injection based on the simultaneous detection of the main components (K, Mg and Asp) and impurities (Na) using a mixed-mode chromatography coupled with charged aerosol detector. To obtain optimal chromatographic separation, the effects of organic content, column temperature, buffer types, pH and concentrations were evaluated. A Response Surface Methodology (RSM) optimal design was performed after single factor experiment. The mixed-mode HPLC method is proved to be a complementary approach to the conventional ion chromatography (IC). The optimized method was successfully validated and applied to the analysis of Asp, K, Na and Mg in PAMA injection with good specificity, linearity, accuracy, and repeatability. The method would be useful for quality control in PAMA injection and other similar drugs, which can provide references for the analysis of drug quality by enterprises and drug regulatory department.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jpba.2018.09.005DOI Listing
November 2018

Compared the physiological response of two petroleum tolerant-contrasting plants to petroleum stress.

Int J Phytoremediation 2018 Aug;20(10):1043-1048

a Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Bioscience and Biotechnology, Shenyang Agricultural University , Shenyang , China.

Petroleum not only benefits the world economy but also contaminates the soil. In order to select the plants tolerant to petroleum, the physiological response of two petroleum tolerant-contrasting plants, Mirabilis jalapa and Orychophragmus violace, were investigated in variation of petroleum-contaminated soils (0, 5, 10, 20, and 40 g petroleum per kg soil) for 120 d. Petroleum degradation rate, seeds germination rate, free proline, and superoxide dismutase and peroxidase activities of M. jalapa were higher than that of O. violace under petroleum stress. However, the decrease rate of soluble protein, plant height, chlorophyll, and root fresh weight was greater in O. violace as compared to M. jalapa. Pearson correlation coefficient analysis was conducted, which indicated that the higher tolerance of M. jalapa was correlated with the higher level of free proline and antioxidative enzyme activities. Besides, the 10 g petroleum per kg soil may be appropriate for petroleum-tolerant plants selection, in which petroleum significantly restrain growth in O. violace but not in M. jalapa.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/15226514.2018.1460303DOI Listing
August 2018