Publications by authors named "Linlin Li"

486 Publications

Association of eating out frequency and other factors with serum uric acid levels and hyperuricemia in Chinese population.

Eur J Nutr 2021 Jul 23. Epub 2021 Jul 23.

Department of Clinical Pharmacology, School of Pharmaceutical Science, Zhengzhou University, Zhengzhou, Henan, People's Republic of China.

Purpose: A significant shift in meal pattern with frequent eating out was closely associated with multiple chronic outcomes, but with limited evidence on hyperuricemia. We aimed to explore the associations between eating out and serum uric acid (SUA) as well as hyperuricemia.

Methods: A total of 29,597 participants were recruited from the Henan Rural Cohort Study. A validated food frequency questionnaire (FFQ) was used to collect dietary data, including the frequency of eating out. Linear regression models were used to examine the association of eating-out frequency with SUA and BMI. Logistic regression and restricted cubic spline were performed to assess the association and dose-response relationship between eating-out frequency and hyperuricemia. The mediation effect of BMI between eating out and the risk of hyperuricemia was evaluated.

Results: Eating out was significantly associated with higher SUA levels in the total population and males (P < 0.001). Multivariate-adjusted odds ratios (ORs) with 95% confidence interval (CIs) of hyperuricemia were 1.26 (1.09, 1.46) for the total population and 1.18 (1.00, 1.40) for males (≥ 7 times/week vs 0 time/week). A non-linear positive dose-response relationship between eating-out frequency and hyperuricemia was observed. Furthermore, BMI played a partial mediating role in the relationship between eating out frequency and hyperuricemia, which explained 30.7% in the total population and 44.8% in males.

Conclusion: Our findings indicated that eating out was associated with increased SUA levels and elevated hyperuricemia risk in rural China, especially in males. Moreover, the relationship was partly mediated by BMI.

Clinical Trials: ChiCTR-OOC-15006699 (2015-07-06).
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http://dx.doi.org/10.1007/s00394-021-02634-9DOI Listing
July 2021

Plasmonic Bi-enhanced ammoniated α-MnS/BiMoO S-scheme heterostructure for visible-light-driven CO reduction.

J Colloid Interface Sci 2021 Jul 18;604:844-855. Epub 2021 Jul 18.

Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, PR China. Electronic address:

Low redox ability and severe photocorrosion limit the photocatalytic activity of metal sulfides. Herein, step-scheme (S-scheme) heterojunction composited by diethylenetriamine (DETA) ammoniated MnS (α-MnS) and BiMoO with Bi surface plasmon resonance (SPR) was successfully fabricated (Bi-5 %M/BMO). This special electron transport structure effectively suppresses the photocorrosion of α-MnS and makes photocatalysts with high redox ability. DETA was protonated to form positively charged ammonium ions and they are easy to combine with acid gas CO, reducing the activation energy of CO, building an efficient catalytic reaction system, and improving CO reduction efficiency. The CO evolution rate of Bi-5 %M/BMO (61.11 μmol gh) is 2.42, 7.89 and 5.01 times greater than that of 5 %M/BMO, pure α-MnS hollow spheres and BiMoO, respectively. This indicates that Bi SPR effect can promote the separation of photon-generated electron-hole pairs dramatically. The ammoniated S-scheme heterostructure decorated with the SPR effect may provide a new perspective to design heterojunction.
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http://dx.doi.org/10.1016/j.jcis.2021.07.064DOI Listing
July 2021

All-Climate Aluminum-Ion Batteries Based on Binder-Free MOF-Derived [email protected]/CNT Cathode.

Nanomicro Lett 2021 Jul 23;13(1):159. Epub 2021 Jul 23.

Nanomaterials Centre, School of Chemical Engineering and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Queensland, 4072, Australia.

Aluminum-ion batteries (AIBs) are promising next-generation batteries systems because of their features of low cost and abundant aluminum resource. However, the inferior rate capacity and poor all-climate performance, especially the decayed capacity under low temperature, are still critical challenges toward high-specific-capacity AIBs. Herein, we report a binder-free and freestanding metal-organic framework-derived [email protected]/carbon nanotube ([email protected]/CNT) as a novel all-climate cathode in AIBs working under a wide temperature window between -25 and 50 °C with exceptional flexibility. The resultant cathode not only drastically suppresses the side reaction and volumetric expansion with high capacity and long-term stability but also greatly enhances the kinetic process in AIBs with remarkable rate capacity (above 151 mAh g at 2 A g) at room temperature. More importantly, to break the bottleneck of the inherently low capacity in graphitic material-based all-climate AIBs, the new hierarchical conductive composite [email protected]/CNT highly promotes the all-climate performance and delivers as high as 117 mAh g capacity even under -25 °C. The well-designed metal sulfide electrode with remarkable performance paves a new way toward all-climate and flexible AIBs.
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http://dx.doi.org/10.1007/s40820-021-00682-8DOI Listing
July 2021

Functional effectiveness of double essential [email protected] starch/microcrystalline cellulose as active antibacterial packaging.

Int J Biol Macromol 2021 Jul 19;186:873-885. Epub 2021 Jul 19.

School of Food and Biological Engineering, Hefei University of Technology, 230009 Hefei, Anhui, PR China; Anhui Institute of Agro-Products Intensive Processing Technology, 230009 Hefei, Anhui, PR China.

In this work, two combinations of double EOs, i.e., α-terpineol: eugenol (α-T:Eu) and carvacrol:eugenol (CA:Eu), are used to develop the active antibacterial films of double [email protected] starch/microcrystalline cellulose ([email protected]). The hydrogen-bonded networks in SC matrix are conducive to thermostability enhancement and the film of SC25 is determined for EO incorporation. The interactions between EOs and SC matrix are also hydrogen bonds and the double [email protected] are smooth at ratio of ≤2:2 for α-T:Eu or CA:Eu. The ultimate film properties are dependent on the incorporated EOs. The release of EOs is well controlled by two mechanisms of diffusion (predominant) and swelling (secondary). Synergetic antibacterial activity occurs on double [email protected] The shelf life of pork can be extended by 1 day at 25 °C by the two typical films of α-T:[email protected] and CA:[email protected] Moreover, [email protected] can be well degraded in humus soil. Thereby, the target films will have great potential in active packaging to extend the shelf life of food.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.094DOI Listing
July 2021

A scalable and reproducible preparation for the antitumor protein TLC, a human-derived telomerase inhibitor.

Protein Expr Purif 2021 Jul 17;187:105942. Epub 2021 Jul 17.

Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou, 313000, China; School of Medicine, Huzhou University, Huzhou, 313000, China. Electronic address:

Telomerase, which is overexpressed in approximately 90% of liver cancer cells, is an ideal target for anti-liver cancer therapy. LPTS, a putative liver tumor suppressor, is the only human-derived protein that can bind telomerase directly and inhibit the extension of telomere activity. Our previous studies demonstrated that TAT-LPTS-LC (TLC), a recombinant protein fused by the C-terminal 133-328 fragment of LPTS and TAT peptides, could be delivered into cells to inhibit telomerase-positive hepatoma cell growth in vitro and in vivo with very low toxicity. In the present study, E. coli strains which expressed TLC in abundance were screened and cultured in a laboratory bioreactor. A reproducible protein separation process was built, and this process was suitable for industrial amplification. The yields of TLC protein were up to 184 mg in one batch with a purity of approximately 95%. The purified TLC protein had a similar inhibitory effect on telomerase activity in vitro compared with those purified by Ni-affinity chromatography. Furthermore, TLC protein could be delivered into the cell nucleus to increase the doubling time of the cell and suppress cell growth in telomerase-positive liver cancer cell lines. Cell growth inhibition was negatively correlated with telomere length, suggesting that TLC is a highly targeted telomerase-telomere anticancer agent. These results will contribute to future preclinical studies of the TLC protein.
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http://dx.doi.org/10.1016/j.pep.2021.105942DOI Listing
July 2021

Isolation and characterization of a new goose orthoreovirus causing liver and spleen focal necrosis in geese, China.

Transbound Emerg Dis 2021 Jul 14. Epub 2021 Jul 14.

Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.

Since July 2020, an infectious disease characterized by liver and spleen white focal necrosis has spreading widely through geese farms in many regions of China. A novel goose orthoreovirus (GRV), designated GRV-GD2020, was isolated from the liver and spleen of dead geese. Phylogenetic analysis and Sequence comparison revealed that all the genes of GRV-GD2020 clustered with other waterfowl-origin orthoreovirus. However, the gene constellation of GRV-GD2020 was not similar to that of any particular strain. Instead, the genomic segments of GRV-GD2020 showed 27.5% to 97.3% similarities to that of other waterfowl-origin orthoreovirus isolates. Waterfowl-origin orthoreovirus infections characterized by liver and spleen focal necrosis has not emerged in recent years. The re-emergence of the disease may be related to the recombination of the genome segments of Muscovy duck reovirus (MDRV), GRV and new-type duck orthoreovirus. In summary, we determined that the GRV-GD2020 strain, causing goose liver and spleen focal necrosis, is a new variant of waterfowl-origin orthoreovirus. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/tbed.14236DOI Listing
July 2021

A new sensor based on thieno[2,3-b]quinoline for the detection of In , Fe and F by different fluorescence behaviors.

Luminescence 2021 Jul 13. Epub 2021 Jul 13.

School of chemistry and chemical engineering, University of Jinan, Jinan, China.

Based on thieno[2,3-b]quinoline-2-carbohydrazide and salicylaldehyde, a novel fluorescent probe (L) was designed and synthesized. L could be used as a multifunctional sensor to sequentially detect In and Fe through fluorescence enhancement and fluorescence quenching in DMF/H O buffer solutions. At the same time, L had good anti-interference ability, which could still detect In and Fe well in the presence of other metal ions. For F , it could be detected by enhancing the fluorescence change caused by the introduction of Al . When other anions were present, the detection of F would not be interfered. The detection limits of In , Fe and F were 1.16×10 M, 2.03×10 M and 7.98×10 M, respectively. The complexation model and sensing mechanism between L and In , Fe and F were confirmed by calculating structural optimization and energy optimization using Gaussian 09 software.
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http://dx.doi.org/10.1002/bio.4119DOI Listing
July 2021

Robust high-bandwidth control of nano-positioning stages with Kalman filter based extended state observer and H control.

Rev Sci Instrum 2021 Jun;92(6):065003

State Key Laboratory of Mechanical System and Vibration, School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

The achievable performance of the piezo-actuated nano-positioning stages is severely limited by the intrinsic nonlinearities of the actuators, the lightly damped resonant mode of the flexure-hinge mechanism, and the external disturbances. To overcome all these limitations, this paper presents a novel robust dual-loop control scheme with a Kalman filter-based extended state observer and H control for nano-positioning stages to implement high-bandwidth tracking operations. In this scheme, the extended state observer (ESO) is first developed and assisted by the identified system model to estimate both the system states and total disturbances, where the estimated disturbance is compensated by the direct feedback. In particular, to further improve the estimation performance, the Kalman filter is thus incorporated into the ESO to optimize the observer gain. Then, the state-feedback-based inner-loop controller is designed via the pole-placement method to damp the resonant mode of nano-positioning stages. Finally, a H robust controller is adopted in the outer-loop to eliminate the influence resulting from the external disturbances, nonlinearities, and unmodeled dynamics on tracking operations. To validate the effectiveness of the proposed approach, comparative experiments are conducted on a piezo-actuated nano-positioning stage. Experimental results demonstrate that the proposed control scheme improves the control bandwidth of the system from 3.6 kHz (the stand-alone H controller) to 5.52 kHz, which is 93.5% of the first resonant frequency of the original system. Moreover, it shows excellent robustness against the variation of system dynamics due to the change in the mounted mass and the external disturbances.
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http://dx.doi.org/10.1063/5.0048870DOI Listing
June 2021

An Integrated Model-Based and Data-Driven Gap Metric Method for Fault Detection and Isolation.

IEEE Trans Cybern 2021 Jul 8;PP. Epub 2021 Jul 8.

This article proposes an integrated approach of model-based and data-driven gap metric fault detection and isolation in a stochastic framework. For actuator and sensor faults, an adaptive Kalman filter combining with the generalized likelihood ratio method is suggested. For component faults, especially incipient faults, the model-based scheme maybe not a good choice due to the existence of disturbances or noises. Hence, a novel data-driven gap metric strategy is presented. The design of the appropriate fault cluster center model and radius via the gap metric technique is put forward to enhance the isolability of the incipient faults. Numerical simulation results are given to demonstrate the effectiveness of the proposed fault detection and isolation algorithm.
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http://dx.doi.org/10.1109/TCYB.2021.3086193DOI Listing
July 2021

Interaction between testosterone and obesity on hypertension: A population-based cross-sectional study.

Atherosclerosis 2021 Jun 24;330:14-21. Epub 2021 Jun 24.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan, PR China. Electronic address:

Background And Aims: We aimed to evaluate the effects of serum testosterone, obesity and their interaction on blood pressure (BP) parameters and hypertension among Chinese rural adults.

Methods: A total of 6199 adults were recruited from the Henan Rural Cohort Study. Serum testosterone was measured by liquid chromatography-tandem mass spectrometry. Logistic regression and linear regression were used to evaluate the association between testosterone, hypertension and BP parameters (including systolic blood pressure (SBP), diastolic blood pressure (DBP), pulse pressure (PP), and mean arterial pressure (MAP)). A generalized linear model was performed to identify the interactive effects of testosterone and obesity on hypertension.

Results: High levels of serum testosterone were associated with a lower prevalence of hypertension in males (odds ratio (OR): 0.69, 95% confidence interval (CI): 0.53, 0.89). After stratification by obesity, observed associations were only found in non-obese males. Each one-unit increase in ln-testosterone was associated with a 1.23 mmHg decrease in SBP, 0.97 mmHg decrease in DBP, and 1.05 mmHg decrease in MAP among males. Moreover, interactive effects between testosterone and obesity on hypertension and BP parameters were found, indicating that protective effects of serum testosterone on hypertension and BP parameters were counteracted and accompanied by increased values of obesity-related indicators in males, and additional testosterone increased BP parameters and prevalence of hypertension at high levels of waist-to-hip ratio and waist-to-height ratio in females.

Conclusions: Elevated levels of serum testosterone were associated with decreased BP parameters and prevalent hypertension in males, and obesity modifying effects of serum testosterone on BP parameters and hypertension.
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http://dx.doi.org/10.1016/j.atherosclerosis.2021.06.906DOI Listing
June 2021

The Accuracy of Transferring Casts in Maximal Intercuspal Position to a Virtual Articulator.

J Prosthodont 2021 Jul 1. Epub 2021 Jul 1.

Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

Purpose: To analyze the accuracy of transferring casts in maximal intercuspal position to a virtual articulator by using transfer plates in the laboratory scanner before and after occlusal optimization.

Material And Methods: Five sets of standard dental casts were mounted on a mechanical articulator in maximal intercuspal position. The number and position of occlusal contacts were determined with 12-μm articulating foil. After a calibration process according to the manufacturer's instructions, the mountings were transferred to a virtual articulator using the transfer plates in a laboratory scanner. The occlusion of the digital casts was determined before and after the occlusal optimization process. Then, the sensitivity and positive predictive value were determined by comparing the occlusal contact points in the virtual articulator with those in the mechanical articulator. To evaluate trueness, the occlusal surface of the maxillary teeth in the mechanical articulator was recorded by polyvinyl siloxane occlusal record in maximal intercuspal position and retained on the mandibular arch. The trueness was calculated as the deviation between the occlusal surface of the maxillary teeth in the mechanical articulator and the virtual articulator. To evaluate precision, one set of the casts was scanned 10 times. And the deviation of the interarch position of the maxillary arches when superimposing the mandibular arches of every 2 different scans was calculated.

Results: The sensitivity before occlusal optimization (0.14 ± 0.15) was significantly lower than that after occlusal optimization (0.82 ± 0.10) (p = 0.003). However, there was no significant difference between the positive predictive value before (0.80 ± 0.45) and after (0.81 ± 0.09) occlusal optimization (p = 0.952). The trueness before (91.0 ± 10.7 µm) and after (75.4 ± 25.2 µm) occlusal optimization had no significant difference (p = 0.249). The precision before occlusal optimization (11.6 ± 3.8 µm) was significantly superior to that after occlusal optimization (75.6 ± 39.2 µm ) (p < 0.001).

Conclusions: The accuracy of transferring casts in maximal intercuspal position to a virtual articulator using transfer plates in the laboratory scanner could be improved after occlusal optimization and can meet the clinical needs for occlusal design and analysis of prostheses.
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http://dx.doi.org/10.1111/jopr.13405DOI Listing
July 2021

Preparation of Superhydrophobic Metal-Organic Framework/Polymer Composites as Stable and Efficient Catalysts.

ACS Appl Mater Interfaces 2021 Jul 29;13(27):32175-32183. Epub 2021 Jun 29.

Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, P.R. China.

Metal-organic frameworks (MOFs), as a chemical platform, combined with multifunctional polymers are of interest in catalytic applications, which can not only inherit the outstanding properties of the two components but also lead to unique synergistic effects. Nonetheless, most MOFs possess varying degrees of water instability, which limits their real application. Herein, we fabricated highly hydrophobic MOF/polymer composites a universal post-synthetic polymerization strategy as efficient catalysts. Polyaniline (PANI) was first hybridized with MOFs by vapor deposition polymerization, and then, hydrophobic molecules were grafted to the PANI by a covalent linking process, thereby forming a superhydrophobic MOF/PANI hybrid material (MOF/PANI-shp). The resultant MOF/PANI-shp not only obtains superior moisture/water resistance without significantly disturbing the original features but also exhibits a novel catalytic selectivity in styrene oxidation because of the accessible sites and synergistic effects. Such a synthetic strategy for the MOF/polymer catalyst opens a new avenue for the design of a unique catalyst with outstanding catalytic efficiency, selectivity, and stability.
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http://dx.doi.org/10.1021/acsami.1c07188DOI Listing
July 2021

A new anesthesia scheme for parathyroidectomy under neuromonitoring: a retrospective cohort study.

Gland Surg 2021 May;10(5):1576-1586

Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun, China.

Background: Parathyroidectomy under nerve monitoring has been carried out for nearly ten years in the China-Japan Union Hospital of Jilin University. We retrospectively evaluated patients' prognosis with secondary hyperparathyroidism (SH) under neuro- and non-neuro-monitored parathyroidectomy anesthesia. The purpose of this study is to summarize and introduce a new anesthesia scheme for parathyroidectomy under nerve monitoring.

Methods: From January 2000 to December 2019, 200 patients with SH in the China-Japan Union Hospital of Jilin University were retrospectively analyzed. Among them, 100 patients underwent parathyroidectomy under neurological monitoring (Group A), and 100 patients underwent parathyroidectomy without neurological monitoring (Group B). The dosage of muscle relaxant, parathyroid hormone (PTH), serum calcium, phosphorus, urea, creatinine, and alkaline phosphatase (ALP) was recorded before surgery (T0), after surgery (T1), at discharge (T2), during skin incision (Ta), at four parathyroidectomies (Tb), and 10 min after total removal. The levels of PTH were measured at four-time points (Tc) and 30 minutes (Td) after complete resection.

Results: After screening and propensity score match (PSM), the data of 92 patients were analyzed. Group A's muscle relaxant dose was significantly less than Group B; the length of hospital stay in Group A was significantly lower than in Group B (P<0.05). The serum calcium levels, phosphorus, urea, and creatinine at T2 in Group A were lower than those in Group B (P<0.05).

Conclusions: Parathyroid nerve monitoring technology combined with preoperative complete anesthesia scheme, anesthesia induction with one time ED95 (95% effective drug dose) cis-atracurium, end breath gas, and sevoflurane maintenance anesthesia under BIS monitoring can improve the prognosis of patients, shorten the length of hospital stay, and is effective and safe.
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http://dx.doi.org/10.21037/gs-21-33DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184376PMC
May 2021

EMT and Cancer Cell Stemness Associated With Chemotherapeutic Resistance in Esophageal Cancer.

Front Oncol 2021 3;11:672222. Epub 2021 Jun 3.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, China.

Drug resistance often occurs after chemotherapy in esophageal cancer patients, leading to cancer metastasis and recurrence. However, the relationship among cancer cell migration, recurrence and drug resistance in esophageal cancer drug-resistant cells has not been clearly explained. In this study, we constructed paclitaxel (PTX)-resistant esophageal cancer cells to explore the causes of drug resistance and poor prognosis after chemotherapy in esophageal cancer. Colony formation assay was used to evaluate the difference of colony formation between parental cells and drug resistance cells. Microsphere formation assay was used to examine the phenotype of stem cells. Wound healing and Transwell assays were used to detect the migration ability of drug-resistant cells. Western blotting and immunofluorescence assays were used to explore the mechanisms. Finally, we used nude mouse xenograft model to explore the tumor characteristics and the expression of relative proteins to verify our findings . Our study demonstrated that the cancer cell stemness characteristics exist in drug-resistant esophageal cancer cells, that expressed the biomarkers of stem cells and were prone to epithelial-mesenchymal transition (EMT). Our results suggested that the expression of EMT biomarkers and stemness-related proteins increased in esophageal cancer cells after continuously using chemotherapeutic drugs for a period of time. This study indicated that simultaneously targeting EMT and stemness could be a better strategy for the treatment of esophageal cancer drug resistance.
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http://dx.doi.org/10.3389/fonc.2021.672222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209423PMC
June 2021

Electroactive Biomaterials and Systems for Cell Fate Determination and Tissue Regeneration: Design and Applications.

Adv Mater 2021 Jun 12:e2007429. Epub 2021 Jun 12.

Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, P. R. China.

During natural tissue regeneration, tissue microenvironment and stem cell niche including cell-cell interaction, soluble factors, and extracellular matrix (ECM) provide a train of biochemical and biophysical cues for modulation of cell behaviors and tissue functions. Design of functional biomaterials to mimic the tissue/cell microenvironment have great potentials for tissue regeneration applications. Recently, electroactive biomaterials have drawn increasing attentions not only as scaffolds for cell adhesion and structural support, but also as modulators to regulate cell/tissue behaviors and function, especially for electrically excitable cells and tissues. More importantly, electrostimulation can further modulate a myriad of biological processes, from cell cycle, migration, proliferation and differentiation to neural conduction, muscle contraction, embryogenesis, and tissue regeneration. In this review, endogenous bioelectricity and piezoelectricity are introduced. Then, design rationale of electroactive biomaterials is discussed for imitating dynamic cell microenvironment, as well as their mediated electrostimulation and the applying pathways. Recent advances in electroactive biomaterials are systematically overviewed for modulation of stem cell fate and tissue regeneration, mainly including nerve regeneration, bone tissue engineering, and cardiac tissue engineering. Finally, the significance for simulating the native tissue microenvironment is emphasized and the open challenges and future perspectives of electroactive biomaterials are concluded.
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http://dx.doi.org/10.1002/adma.202007429DOI Listing
June 2021

Three-dimensional ATUM-SEM reconstruction and analysis of hepatic endoplasmic reticulum‒organelle interactions.

J Mol Cell Biol 2021 May 28. Epub 2021 May 28.

National Laboratory of Pattern Recognition, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.

The endoplasmic reticulum (ER) is a contiguous and complicated membrane network in eukaryotic cells, and membrane contact sites (MCSs) between the ER and other organelles perform vital cellular functions, including lipid homeostasis, metabolite exchange, calcium level regulation, and organelle division. Here, we establish a whole pipeline to reconstruct all ER, mitochondria, lipid droplets, lysosomes, peroxisomes, and nuclei by automated tape-collecting ultramicrotome scanning electron microscopy (ATUM-SEM) and deep learning techniques, which generates an unprecedented 3D model for mapping liver samples. Furthermore, the morphology of various organelles and the MCSs between the ER and other organelles are systematically analyzed. We found that the ER presents with predominantly flat cisternae and is knitted tightly all throughout the intracellular space and around other organelles. In addition, the ER has a smaller volume-to-membrane surface area ratio than other organelles, which suggests that the ER could be more suited for functions that require a large membrane surface area. Our data also indicate that ER‒mitochondria contacts are particularly abundant, especially for branched mitochondria. Our study provides 3D reconstructions of various organelles in liver samples together with important fundamental information for biochemical and functional studies in the liver.
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http://dx.doi.org/10.1093/jmcb/mjab032DOI Listing
May 2021

Recent Progress of the Practical Applications of the Platinum Nanoparticle-Based Electrochemistry Biosensors.

Front Chem 2021 3;9:677876. Epub 2021 May 3.

School of Life Sciences, Changchun Normal University, Changchun, China.

The detection of biomolecules using various biosensors with excellent sensitivity, selectivity, stability, and reproducibility, is of great significance in the analytical and biomedical fields toward achieving their practical applications. Noble metal nanoparticles are favorable candidates due to their unique optical, surface electrical effect, and catalytic properties. Among these noble metal nanoparticles, platinum nanoparticles (Pt NPs) have been widely employed for the detection of bioactive substances such as glucose, glutamic acid, and hormones. However, there is still a long way to go before the potential challenges in the practical applications of biomolecules are fully overcome. Bearing this in mind, combined with our research experience, we summarized the recent progress of the Pt NP-based biosensors and highlighted the current problems that exist in their practical applications. The current review would provide fundamental guidance for future applications using the Pt NP-based biosensors in food, agricultural, and medical fields.
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http://dx.doi.org/10.3389/fchem.2021.677876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128108PMC
May 2021

Management of Follow-Up With Preterm Infants During the Outbreak in China.

Front Pediatr 2021 29;9:637275. Epub 2021 Apr 29.

Department of Pediatrics, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.

Coronavirus disease 2019 (COVID-19) swept Wuhan in January 2020. Other cities in China also suffered during the pandemic. Routine medical services were conducted in the Neonatal Intensive Unit (NICU) as usual, but the follow-up after discharge was seriously affected. To investigate the feasibility and effectiveness of a combination of online and face-to-face follow-up for preterm infants during the COVID-19 epidemic and to explore a follow-up pattern that can provide follow-up services while maximizing the protection of preterm infants and soothing the fear of their parents. Preterm infants ( = 35) whose first follow-up appointment was scheduled from February 1 to April 30, 2020, and preterm infants ( = 43) in the NICU follow-up group who were discharged from January 1, 2018, to January 31, 2020, who had a second or later routine follow-up appointment scheduled from February 1 to April 30, 2020, were enrolled. We provided a combination of online and face-to-face follow-up for preterm infants surveyed with the Wenjuanxing platform before and after the online follow-up and compared the first-time follow-up rate between the outbreak and the same period of the previous year. Feeding and oral medicine and supplements were the most concerning problems of the parents of preterm infants. The anxiety level of the family was significantly decreased after online follow-up ( < 0.05). A total of 96.8% of parents were satisfied or very satisfied with online follow-up, and 95.2% of parents thought that online follow-up had answered all their questions. Only 35.5% of parents thought online follow-up could replace face-to-face follow-up. The combination of online and face-to-face follow-up alleviated the anxiety of the parents during the outbreak and achieved a similar first-time follow-up rate as the same period in 2019.
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http://dx.doi.org/10.3389/fped.2021.637275DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116626PMC
April 2021

Hierarchical TiCT MXene/Carbon Nanotubes for Low Overpotential and Long-Life Li-CO Batteries.

ACS Nano 2021 May 12;15(5):8407-8417. Epub 2021 May 12.

Jiangsu Key Laboratory of Electrochemical Energy Storage Technologies, College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China.

Electrochemical carbon dioxide conversion at ambient temperature is an efficient route to synchronously provide a continuous power supply and produce useful chemicals such as carbonates. Rigid catalysts with rational morphological and structural design are used to overcome the sluggish reaction kinetics and contribute to a better cycle life in Li-CO batteries. In this report, a two-dimensional TiCT MXene/carbon heterostructure assembled parallel-aligned tubular architecture was delicately synthesized through a self-sacrificial templating method and delivered an ultralow overpotential of 1.38 V at 0.2 A·g. The heterostructure that inherited the high catalytic performance of TiCT MXene and the outstanding stability of carbon material promoted the adsorption of CO and accelerated the decomposition of lithium carbonate, which was proved by and characterizations and density functional theory calculations. The tubular architecture with large surface area was demonstrated to provide a high durability for long cycle life and ensure good contacts among gas, electrolyte, and electrode.
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http://dx.doi.org/10.1021/acsnano.0c10558DOI Listing
May 2021

Automatic wavelet-based 3D nuclei segmentation and analysis for multicellular embryo quantification.

Sci Rep 2021 May 10;11(1):9847. Epub 2021 May 10.

Department of Agriculture and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA.

Identification of individual cells in tissues, organs, and in various developing systems is a well-studied problem because it is an essential part of objectively analyzing quantitative images in numerous biological contexts. We developed a size-dependent wavelet-based segmentation method that provides robust segmentation without any preprocessing, filtering or fine-tuning steps, and is robust to the signal-to-noise ratio. The wavelet-based method achieves robust segmentation results with respect to True Positive rate, Precision, and segmentation accuracy compared with other commonly used methods. We applied the segmentation program to zebrafish embryonic development IN TOTO for nuclei segmentation, image registration, and nuclei shape analysis. These new approaches to segmentation provide a means to carry out quantitative patterning analysis with single-cell precision throughout three dimensional tissues and embryos and they have a high tolerance for non-uniform and noisy image data sets.
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http://dx.doi.org/10.1038/s41598-021-88966-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110989PMC
May 2021

Acceleration of PDE-Based Biological Simulation Through the Development of Neural Network Metamodels.

Curr Pathobiol Rep 2020 Dec 6;8(4):121-131. Epub 2020 Nov 6.

Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907.

Purpose Of Review: Partial differential equation (PDE) mathematical models of biological systems and the simulation approaches used to solve them are widely used to test hypotheses and infer regulatory interactions based on optimization of the PDE model against the observed data. In this review, we discuss the ability of powerful machine learning methods to accelerate the parametric screening of biophysical informed- PDE systems.

Recent Findings: A major shortcoming in more broad adaptation of PDE-based models is the high computational complexity required to solve and optimize the models and it requires many simulations to traverse the very high-dimensional parameter spaces during model calibration and inference tasks. For instance, when scaling up to tens of millions of simulations for optimization and sensitivity analysis of the PDE models, compute times quickly extend from months to years for sufficient coverage to solve the problems. For many systems, this brute-force approach is simply not feasible. Recently, neural network metamodels have been shown to be an efficient way to accelerate PDE model calibration and here we look at the benefits and limitations in extending the PDE acceleration methods to improve optimization and sensitivity analysis.

Summary: We use an example simulation to quantitatively and qualitatively show how neural network metamodels can be accurate and fast and demonstrate their potential for optimization of complex spatiotemporal problems in biology. We expect these approaches will be broadly applied to speed up scientific research and discovery in biology and other systems that can be described by complex PDE systems.
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http://dx.doi.org/10.1007/s40139-020-00216-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104327PMC
December 2020

Long-term exposure to particulate matter and residential greenness in relation to androgen and progesterone levels among rural Chinese adults.

Environ Int 2021 08 4;153:106483. Epub 2021 May 4.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan, PR China. Electronic address:

Background: Population-based studies on the associations of long-term exposure to particulate matter (PM) with androgen and progesterone are still scant. Residential greenness is benefits health by promoting physical activity, reducing air pollution, and improving mental health, but it remains unclear whether it is related to androgen and progesterone levels among humans.

Aims: This study aimed to explore the individual and interactive effects of PM and residential greenness on serum testosterone and progesterone levels among rural Chinese adults.

Methods: A total of 6017 subjects were recruited from the baseline of the Henan Rural Cohort Study in 2016. Serum testosterone and progesterone were measured with liquid chromatography-tandem mass spectrometry. Particulate matters (PM) (PM, PM, and PM) were assessed by machine learning algorithms. Residential greenness was assessed using the normalized difference vegetation index (NDVI) within 500-m, 1000-m, and 3000-m buffers around participants' residences. The effects of air pollutants and residential greenness and their interaction on serum testosterone and progesterone levels were assessed using linear mixed-effects models with township as a random intercept.

Results: After adjusting for potential confounding factors, a 1 μg/m increase in PM or PM was associated with a 0.037 or 0.030 ng/ml increase in serum testosterone, respectively, in females and with a 0.111 or 0.182 ng/ml decrease in serum progesterone, respectively, in males. A 1 μg/m increase in PM, PM or PM was associated with a 0.222, 0.306, or 0.295 ng/ml decrease in serum progesterone, respectively, among females. Moreover, a 0.1-unit increase in NDVI was associated with a 0.310 ng/ml increase in serum testosterone and a 0.170 ng/ml increased in serum progesterone in males, as well as with a 0.143 ng/ml increase in serum progesterone in females. Interaction effects of PM and residential greenness on serum testosterone and progesterone levels were observed, indicating that the effects of residential greenness on serum testosterone and progesterone were modified by high levels of PM. In addition, physical activity significantly mediated 2.92% of the estimated association between greenness and testosterone levels.

Conclusions: Our study suggested that long-term exposure to PM was positively associated with serum testosterone in males but negatively associated with progesterone levels in both genderssin. In addition, positive associations of residential greenness with serum testosterone and progesterone levels were observed, but they were modified by high levels of PM. Furthermore, the estimated effects of residential greenness on testosterone levels were partly mediated by physical activity.
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http://dx.doi.org/10.1016/j.envint.2021.106483DOI Listing
August 2021

A supramolecular nanocarrier for efficient cancer imaging and therapy by targeting at matriptase.

J Control Release 2021 06 21;334:153-163. Epub 2021 Apr 21.

College of Chemistry, Fuzhou University, Fujian 350116, China. Electronic address:

Human serum albumin (HSA), a versatile protein carrier for endogenous and exogenous compounds, is a proven macromolecule to form nanoparticles for drug delivery. To render HSA carrier specificity toward tumors, we designed a recombinant HSA protein fused with Kunitz domain 1 (KD1) of hepatocyte growth factor activator inhibitor type 1, which targets to matriptase, a type II transmembrane serine protease overexpressed on tumor cell surface. The carrier was thus named matriptase targeting carrier (MTC). In this study, we showed that MTC displayed the same inhibitory potency as the KD1 againast matriptase, demonstrating the HSA fusion did not affect the KD1 targeting potency. For tumor optical imaging and ablation, MTC was prepared as nanoparticle drug carrier by a novel method via denaturation and refolding to incorporate photosensitizer, CPZ. This matriptase targeting nanoparticles, CPZ:[email protected], showed high specificity and cytotoxicity for matriptase-overexpressing cancer cells in vitro. In tumor-bearing mice, CPZ:[email protected] demonstrated selective accumulation and high retention in matriptase-overexpressing tumor. Under illumination, the nanoparticles significantly reduced tumor volumes (79.6%) as compared to saline control. These findings showed that this supramolecular nanocarrier, a new type of tumor targeting self-assembly nanoparticle, had potential as a highly efficient tumor targeting drug carrier for imaging and therapy.
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http://dx.doi.org/10.1016/j.jconrel.2021.04.019DOI Listing
June 2021

The role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells.

Cell Death Dis 2021 04 15;12(4):408. Epub 2021 Apr 15.

Cancer Research Institute, Guangdong Provincial Key Laboratory of Cancer Immunotherapy, Guangzhou Key Laboratory of Tumor Immunology Research, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China.

One of the malignant transformation hallmarks is metabolism reprogramming, which plays a critical role in the biosynthetic needs of unchecked proliferation, abrogating cell death programs, and immunologic escape. However, the mechanism of the metabolic switch is not fully understood. Here, we found that the S-nitrosoproteomic profile of endogenous nitrogen oxide in ovarian cancer cells targeted multiple components in metabolism processes. Phosphofructokinase (PFKM), one of the most important regulatory enzymes of glycolysis, was S-nitrosylated by nitric oxide synthase NOS1 at Cys351. S-nitrosylation at Cys351 stabilized the tetramer of PFKM, leading to resist negative feedback of downstream metabolic intermediates. The PFKM-C351S mutation decreased the proliferation rate of cultured cancer cells, and reduced tumor growth and metastasis in the mouse xenograft model. These findings indicated that S-nitrosylation at Cys351 of PFKM by NOS1 contributes to the metabolic reprogramming of ovarian cancer cells, highlighting a critical role of endogenous nitrogen oxide on metabolism regulations in tumor progression.
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http://dx.doi.org/10.1038/s41419-021-03681-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050300PMC
April 2021

miRNA-138-5p suppresses cigarette smoke-induced apoptosis in testicular cells by targeting Caspase-3 through the Bcl-2 signaling pathway.

J Biochem Mol Toxicol 2021 Apr 15:e22783. Epub 2021 Apr 15.

Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang, PR China.

Long-term cigarette smoking (CS) can cause testicular toxicity, which interferes with normal spermatogenesis and leads to male infertility. One possible mechanism for this is the activation of the apoptosis signaling pathway, which leads to the irreversible apoptosis of testicular cells. However, the exact mechanism for this is not completely understood. Cell viability, cell apoptosis, and lactate dehydrogenase release assays were performed to elucidate the function of micro RNA (miRNA) in the pathogenesis of male testicular cell injury induced by CS. The results suggested that testicular cell injury was associated with CS both in vitro and in vivo. CS extract (CSE)-treated Leydig and Sertoli cells showed noticeable apoptosis. Based on the results of Agilent miRNA microarray and bioinformatics analyses, miRNA-138-5p was used in subsequent experiments. Quantitative polymerase chain reaction and Western blot assays showed a negative correlation between miR-138-5p and Caspase-3 expression. Transfection of miR-138-5p mimic significantly inhibited apoptosis and downregulated the expression of Caspase-3 in TM3 and TM4 cells. Furthermore, a dual-luciferase reporter assay demonstrated that miR-138-5p directly targeted Caspase-3 to regulate the apoptosis of testicular cells mediated by CSE. In addition, overexpression of miR-138-5p markedly downregulated the expression of p53 and Bak, which played critical roles in the Bcl-2 pathway. These results demonstrate that miRNA-138-5p inhibits CS-induced apoptosis in testicular cells by targeting Caspase-3 through the Bcl-2 signaling pathway.
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http://dx.doi.org/10.1002/jbt.22783DOI Listing
April 2021

Removal of inorganic arsenic from aqueous solution by Fe-modified ceramsite: batch studies and remediation trials.

Water Sci Technol 2021 Apr;83(7):1522-1534

School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao, Shandong Province 266033, China E-mail:

During sediment remediation, adsorbent addition is an effective technology for the removal of contaminants but the cost is often high. In this study, a low-cost adsorbent, ceramsite, made from contaminated riverbed sediment was synthesized. The Fe-modified ceramsite (FMC) was used as adsorbent to remove arsenate from aqueous solutions and reduce the inorganic arsenic release from contaminated sediments. Kinetic studies showed that chemisorption mainly governed the adsorption process while batch studies yielded the theoretical adsorption capacity for arsenate of 10.63 mg/g at pH = 7 condition. Co-existing anions and pH have no significant impact on the adsorption process. In the regeneration studies, 91, 86, and 80% of the adsorption capacity were recovered in 3 cycles. In-situ remediation trials revealed that the addition of the adsorbent to sediment surface significantly reduced the release of inorganic arsenic into aqueous system, with a reduction efficiency of 86%. Furthermore, the species of the arsenic in the surface layer was significantly inactivated from an active state to a stable state. These findings highlight the application of the FMC as a facile and cost-effective adsorbent for containment of arsenic in solutions and sediments, demonstrating that they are highly applicable for practical cases.
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http://dx.doi.org/10.2166/wst.2021.076DOI Listing
April 2021

Characterization of the complete mitochondrial genome of the hybrid grouper (♀ × ♂) with phylogenetic consideration.

Mitochondrial DNA B Resour 2021 Mar 18;6(3):1034-1035. Epub 2021 Mar 18.

Laizhou Mingbo Aquatic Co., Ltd, Yantai, China.

Here we isolated and characterized the complete mitochondrial genome of the hybrid grouper (♀ × ♂). It is 16,503 bp long and contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. The nucleotide composition is 29.08% of A, 29.03% of C, 15.66% of G and 26.23% of T, with 55.31% A + T. The phylogenetic analysis by neighbor-joining (NJ) method reveals that the hybrid offspring has a closer relationship to .
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http://dx.doi.org/10.1080/23802359.2021.1899071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995862PMC
March 2021

Association between Coalmine Dust and Mortality Risk of Lung Cancer: A Meta-Analysis.

Biomed Res Int 2021 8;2021:6624799. Epub 2021 Mar 8.

Department of Clinical Epidemiology, First Affiliated Hospital, China Medical University, Shenyang 110001, China.

Background: Evidence on the carcinogenicity of coalmine dust in occupational settings is still conflicting. Therefore, we conducted this research to evaluate the mortality risk of lung cancer for coalminers exposed to occupational dust when compared to population with no or low dust exposure.

Methods: Databases of PubMed and Chinese National Knowledge Infrastructure as well as reference lists were searched updated to September 18, 2020. The enrolled articles should report lung cancer mortality risk for coalminers exposed to occupational dust. Basic information was extracted such as the author and publication year, area and ethnicity, the type and estimates of outcome, duration of follow-up, and the study design. The checklists from Agency for Healthcare Research and Quality and the Newcastle-Ottawa Scale were used for the assessment of quality and bias risk for descriptive studies, cohort studies, and case control studies, respectively. The overall relative risks were calculated while Begg's and Egger's tests and sensitivity analysis were performed to explore potential heterogeneity sources. Metaregression and subgroup analyses were also conducted to give more detailed information for the correlation between dust exposure and lung cancer mortality.

Results: A total of 19 articles with 22 different studies (descriptive study, case control study, and cohort study) including 8909 observed deaths from 1964 to 2017 were enrolled with a significant heterogeneity ( = 95%, < 0.001). The pooled relative risk of mortality from lung cancer was 1.16 (1.03-1.30) for coalminers. Results of metaregression analysis indicated that the high heterogeneity among these enrolled studies might be caused by the ethnicity differences ( = 0.011). Subgroup analysis also indicated that the pooled estimate for Asian population in China was 4.94 (3.95-6.17) with = 39.3% and = 0.192. All these results suggested that exposure to occupational dust would significantly increase the mortality risk of lung cancer, especially for Asian population in China, which should be measured and controlled more strictly. . This systematic review and meta-analysis provides high-quality evidence that exposure to occupational dust might increase the mortality risk of lung cancer, especially for Asian populations in China. The magnitude of this effect is of major public health importance in view of the ubiquitous existence of coalmining industry in China and even in the world. However, these pooled estimates should be interpreted cautiously because of the high heterogeneity among these publications. . This study was supported by the National Key Research and Development Program of China (Grant No. 2016YFC1302501).
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http://dx.doi.org/10.1155/2021/6624799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7963907PMC
May 2021

Long-term exposure to PM and PM is associated with serum cortisone level and meat intake plays a moderation role.

Ecotoxicol Environ Saf 2021 Jun 16;215:112133. Epub 2021 Mar 16.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan, PR China. Electronic address:

Background: Although short-term exposure to particulate matter (PM) was associated with increased glucocorticoids (GCs) levels, available evidence on associations of long-term exposure to PM and GCs levels is still scant. Previous studies has showed that meat intake is associated with sex hormones levels, but it is unknown whether meat intake is associated with GCs levels. Furthermore, the role of meat intake in the associations between PM and GCs levels remains unclear.

Aims: The aims of this study were to explore the associations of long-term exposure to PM and GCs levels among Chinese rural adults, and the role of meat intake in these associations.

Materials And Methods: A total of 6223 subjects were recruited from the Henan Rural Cohort Study. Serum GCs levels were measured with liquid chromatography-tandem mass spectrometry. The concentrations of PM (PM and PM) for each subject were assessed with machine learning algorithms. The food frequency questionnaire (FFQ) was used to obtain each participant' information on meat intake. The effects of PM and meat intake on GCs levels were assessed using generalized linear models. In addition, modification analyses were performed to identify the role of meat intake played in the associations of PM with serum GCs levels.

Results: Per 1 μg/m increment in PM or PM concentration was associated with a 0.364 ng/ml (95% confidence interval (CI): 0.234, 0.494) or 0.227 ng/ml (95%CI: 0.110, 0.343) increase in serum cortisone, respectively. In addition, the moderation effects of total meat intake and red meat intake on the associations of long-term exposure to PM or PM with serum cortisone were observed (P < 0.05), indicating that individuals who had high levels of PM or PM and meat intake were more susceptible to have a higher state of serum cortisone.

Conclusions: Our findings suggested that long-term exposure to PM or PM was associated with serum cortisone. Moreover, meat intake was found to be a significant moderator in the association of PM or PM with serum cortisone levels.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112133DOI Listing
June 2021
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