Publications by authors named "Linjie Liu"

21 Publications

  • Page 1 of 1

Association of Extracellular Signal-Regulated Kinase Genes With Myopia: A Longitudinal Study of Chinese Children.

Front Genet 2021 27;12:654869. Epub 2021 May 27.

The Eye Hospital, Wenzhou Medical University, Wenzhou, China.

Objective: The present study was designed to investigate whether the extracellular signal-regulated kinase (ERK) signaling pathway, a downstream component of dopamine signaling, is involved in myopia among Chinese children.

Methods: During a 3.5-year follow-up, 488 primary school students were enrolled in this study. Non-cycloplegic spherical equivalent refraction (SE) and other ocular parameters were assessed. Four variants of four genes in the ERK signaling pathway were selected: rs6495367, rs1550870, rs11178469, and rs6554163. SNPscan was used to genotype single-nucleotide polymorphisms (SNPs). PLINK software was used to assess the associations of the genetic variants with the occurrence or development of myopia, SE, and other ocular parameters. We created a protein-protein interaction (PPI) network and microRNA (miRNA)-gene network using String and Cytoscape and conducted enrichment analyses on the genes in these networks.

Results: In total, 426 children (baseline age: 7.28 ± 0.26 years; 236 (55.4%) boys and 190 girls) wereenrolled. After adjusting for confounding factors with 10,000 permutations, children with the CT or TT genotype of rs155087 were more susceptible to myopia than those with the CC genotype (adjusted = 0.011). Additionally, rs155087 was correlated with significant myopic shift and increasing axial length (AL) and lens thickness (LT) but had a negative effect on central corneal thickness (CCT). rs6495367 was negatively associated with myopic shift (additive: adjusted = 0.034; dominant: adjusted = 0.020), myopic SE and AL. rs6554163 TA or AA was negatively associated with increasing LT (adjusted = 0.033). Evaluation of the effects of SNP-SNP combinations on incident myopia revealed a statistically significant one-locus model: rs1550870 [cross-validation consistency (CVC) = 10/10, adjusted = 0.0107]. The genes in the PPI and miRNA-gene interaction networks were subjected to enrichment analyses, which suggested that these genes are involved mainly in eye development and dopaminergic synapse-related processes.

Conclusion: We identified genetic variants of crucial ERK signaling pathway genes that were significantly correlated with myopia and ocular parameter alterations in Chinese children. A combination of gene and miRNA functional analyses with enrichment analyses highlights the regulatory effects associated with ocular development and dopamine biological functions. This study offers novel clues to understand the role of dopamine in the molecular mechanisms of myopia.
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http://dx.doi.org/10.3389/fgene.2021.654869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191505PMC
May 2021

Association of Myopia and Genetic Variants of and in the TGF-β Signaling Pathway: A Longitudinal Study in Chinese School-Aged Children.

Front Cell Dev Biol 2021 28;9:628182. Epub 2021 Apr 28.

Eye Hospital, Wenzhou Medical University, Wenzhou, China.

Background: Myopia is a complex multifactorial condition which involves several overlapping signaling pathways mediated by distinct genes. This prospective cohort study evaluated the associations of two genetic variants in the TGF-β signaling pathway with the onset and progression of myopia and ocular biometric parameters in Chinese school-aged children.

Methods: A total of 556 second grade children were examined and followed up for 3.5 years. Non-cycloplegic refraction and ocular biometric parameters were measured annually. Multivariate regression analysis was used to assess the effect of the rs10760673 and rs7550232 variants on the occurrence and progression of myopia. A 10,000 permutations test was used to correct for multiple testing. Functional annotation of single nucleotide polymorphisms (SNPs) was performed using RegulomeDB, HaploReg, and rVarBase.

Results: A total of 448 children were included in the analysis. After adjustments for gender, age, near work time and outdoor time with 10,000 permutations, the results indicated that the C allele and the AC or CC genotypes of rs7550232 adjacent to were associated with a significantly increased risk of the onset of myopia in two genetic models (additive: ' = 0.022; dominant: ' = 0.025). Additionally, the A allele and the AA or AG genotypes of rs10760673 of were associated with a significant myopic shift (additive: ' = 0.008; dominant: ' = 0.028; recessive: ' = 0.027). Furthermore, rs10760673 was associated with an increase in axial length (AL) (' = 0.013, β = 0.03) and a change in the ratio of AL to the corneal radius of curvature (AL/CRC) (' = 0.031, β = 0.003). Analysis using RegulomeDB, HaploReg, and rVarBase indicated that rs7550232 is likely to affect transcription factor binding, any motif, DNase footprint, and DNase peak.

Conclusion: The present study indicated that rs10760673 and rs7550232 may represent susceptibility loci for the progression and onset of myopia, respectively, in school-aged children. Associations of the variants of the and genes with myopia may be mediated by the TGF-β signaling pathway; this hypothesis requires validation in functional studies. This trial was registered as ChiCTR1900020584 at www.Chictr.org.cn.
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http://dx.doi.org/10.3389/fcell.2021.628182DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115727PMC
April 2021

Relationship between Myopia Progression and School Entrance Age: A 2.5-Year Longitudinal Study.

J Ophthalmol 2021 30;2021:7430576. Epub 2021 Mar 30.

The Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.

Objective: To investigate the association between myopia progression and school entrance age among Chinese schoolchildren and to suggest a more appropriate school age.

Methods: 1,463 children aged six to nine years from Wenzhou, China, were examined and followed up for two and a half consecutive years. Their noncycloplegic refraction was measured twice each year by using an automatic refractometer; axial length (AL) and corneal radius of curvature (CRC) were tested annually by using the IOLMaster for 2.5 years. The questionnaires were completed by the children to collect detailed information regarding risk factors. Here, myopia is defined as a spherical equivalent less than -1.0D.

Results: The changes in spherical equivalent (SE) of 7-year-old children in grade 1 and grade 2 were -0.45D and -0.56D, while changes in AL were 0.59 mm and 0.62 mm, respectively. The SE changes of 8-year-old children in grade 2 and grade 3 were -0.54D and -0.75D; meanwhile, the AL changes were 0.57 mm and 0.61 mm, respectively. Significant statistical differences were observed in ocular biological structure parameters, except for corneal radius of curvature (CRC) or anterior chamber depth (ACD), among children with the same age in different grades during this study. The prevalence of myopia was also significantly higher in higher grades for children with same age.

Conclusions: Myopia is related to children's school entrance age. Children who start school in an earlier age are more likely to suffer from myopia, and the progression of myopia can be considerably faster. Therefore, it is recommended to enter school after the age of 7.
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http://dx.doi.org/10.1155/2021/7430576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026293PMC
March 2021

Longitudinal association between myopia and parental myopia and outdoor time among students in Wenzhou: a 2.5-year longitudinal cohort study.

BMC Ophthalmol 2021 Jan 6;21(1):11. Epub 2021 Jan 6.

The Eye Hospital of Wenzhou Medical University, 270 West Xueyuan Road, Wenzhou, Zhejiang, 325027, China.

Background: To evaluate the impact of parental myopia and outdoor time on myopia among students in Wenzhou.

Methods: We examined 1388 primary students from first grade to third grade in Wenzhou from September 2012 to March 2015. We performed noncycloplegic refractometry on each student every six months and axial length (AL) measurements every year. At the commencement of our study, children were asked to complete a questionnaire regarding near work activity and outdoor activity, whereas parents were asked to complete a self-administered questionnaire regarding their background circumstances and their history of myopia.

Results: A total of 1294 students (93.2%) returned for follow-up examinations. Children with initial and final no myopia spent more time on outdoor activities than those with new onset myopia (1.92 vs. 1.81 h/d, p = 0.022), and elongation of AL in children with a high level (> 2.5 h/day) of outdoor time (0.22 ± 0.13 mm/Y) was less than those with a low level (≤ 1.5 h/day) of outdoor time (0.24 ± 0.14 mm/Y, p = 0.045). The proportion of rapid myopia progression (≤-0.5D/Y) was 16.7%, 20.2% and 31.5% among the children with no myopic parent, one myopic parent and two myopic parents, respectively (X = 28.076, p < 0.001), and the elongation of AL in children among different numbers of myopic parents was significantly different (p < 0.001). A high level of outdoor time was a protective factor for children with one myopic parent (HR 0.49, 95% CI 0.27-0.88; p = 0.018).

Conclusions: In this sample, parental myopia and outdoor time were associated with myopia in children. A high level of outdoor time was a protective factor for children with one myopic parent.
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http://dx.doi.org/10.1186/s12886-020-01763-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789164PMC
January 2021

Genome-Wide Association of Genetic Variants With Refraction, Axial Length, and Corneal Curvature: A Longitudinal Study of Chinese Schoolchildren.

Front Genet 2020 25;11:276. Epub 2020 Mar 25.

The Eye Hospital, Wenzhou Medical University, Wenzhou, China.

Background: Myopia is a common eye disorder that is approaching epidemic proportions worldwide. A genome-wide association study identified (rs12511037), (rs13215566), and (rs12206610) as being associated with refractive error in Asian populations. The present study investigated the associations between these three genetic variants and the occurrence and development of myopia, spherical equivalent refraction (SER), axial length (AL), and corneal curvature (CC) in a cohort of southeastern Chinese schoolchildren.

Methods: We examined and followed 550 children in grade 1 enrolled in the Wenzhou Epidemiology of Refractive Error (WERE) project. During the 4-year follow-up, non-cycloplegic refraction was evaluated twice each year, and the AL and CC were measured once every year. Age, sex, and the amounts of time spent on near work and outdoors were documented with a questionnaire. Sanger DNA sequencing was used to genotype single nucleotide polymorphisms (SNPs). SNPtest software was used to identify potential genetic variants associated with myopia, SER, AL, and CC. Ten thousand permutations were used to correct for multiple testing.

Results: In total, 469 children, including 249 (53.1%) boys and 220 (46.9%) girls, were included in analyses. The mean age of all the children was 6.33 ± 0.48 years. After adjusting for age, sex, time spent on near work and time spent outdoors, neither the genotypes nor the allele frequencies of the three SNPs were significantly associated with myopic shift, incident myopia or the change in SER. After adjusting for age, sex, near-work time and outdoor time with 10,000 permutations, the genotype (rs12511037) was associated with an increase in AL ('-values for the dominant, recessive, additive and general models were 0.0032, 0.0275, 0.0045, and 0.0099, respectively); the genotype (rs12206610) was associated with a change in CC in the additive (' = 0.0096), dominant (' = 0.0096), and heterozygous models (' = 0.0096).

Conclusion: These findings preliminarily indicate that SNP rs12511037 and SNP rs12206610 are etiologically relevant for ocular traits, providing a basis for further exploration of the development of myopia and its molecular mechanism. However, elucidating the role of and in the pathogenesis of myopia requires further animal model and human genetic epidemiology studies. This trial is registered as ChiCTR1900020584 at www.Chictr.org.cn.
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http://dx.doi.org/10.3389/fgene.2020.00276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7109285PMC
March 2020

Protective effects of fisetin against myocardial ischemia/reperfusion injury.

Exp Ther Med 2020 May 6;19(5):3177-3188. Epub 2020 Mar 6.

Department of Pharmacy, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi 710077, P.R. China.

The underlying mechanism of the myocardial protective effect of fisetin was studied in a rat ischemia/reperfusion injury model. Sprague-Dawley rats were randomly assigned to seven groups and pretreated with different solutions by gavage administration. A rat model of cardiac ischemia/reperfusion injury was established. Plasma levels of Von Willebrand factor (vWF) were determined by ELISA, flow cytometry was used to determine the level of cardiomyocyte apoptosis and 2,3,5-triphenyltetrazolium staining was used to determine the size of myocardial infarcts. Hematoxylin and eosin-stained sections of myocardial tissues were examined for pathological changes. Expressions of nuclear factor (NF)-κB and matrix metallopeptidase 9 (MMP-9) were measured by immunohistochemistry. Compared with the model group, rats pretreated with fisetin, quercetin and aspirin showed significant prolongation of clotting time, prothrombin time, thrombin time and activated partial thromboplastin time. Fisetin treatment better maintained the integrity of myocardial fibers and nuclear integrity, reduced the percentage of apoptotic myocardial cells, inhibited expression of NF-κB, decreased the loss of MMP-9 and reduced nuclear translocation of NF-kB. Rats pretreated with fisetin also demonstrated a significant decrease in plasma levels of vWF. In addition, the protective effect of fisetin on myocardial cells was found to be dose dependent.
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http://dx.doi.org/10.3892/etm.2020.8576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132235PMC
May 2020

Mitochondrial unfolded protein response gene CLPP changes mitochondrial dynamics and affects mitochondrial function.

PeerJ 2019 2;7:e7209. Epub 2019 Jul 2.

Department of Endocrinology, Zhongnan Hospital of Wuhan University, Wuhan, China.

Mitochondrial dynamics is associated with mitochondrial function, which is associated with diabetes. Although an important indicator of the mitochondrial unfolded protein response, to the best of our knowledge, and its effects on mitochondrial dynamics in islet cells have not been studied to date. We analyzed the effects of on mitochondrial dynamics and mitochondrial function in the mice islet β-cell line Min6 under high glucose and high fat conditions. Min6 cells were assigned to: Normal, HG, HG+NC, HG+si, HF, HF+NC and HF+ si groups. High glucose and high fat can promote the mRNA and protein expression of in mitochondria. The increase of mitochondrial fission, the decrese of mitochondrial fusion, and the damage of mintocondrial ultrastructure were significant in the si cell groups as compared to no-si treated groups. Meanwhile, mitochondrial functions of MIN6 cells treated with si were impaired, such as ATP decreased, ROS increased, mitochondrial membrane potential decreased. In addition, cell insulin secretion decreased and cell apoptosis rate increased in si groups. These results revealed that mitochondrial unfolded protein response gene alleviated high glucose and high fat-induced mitochondrial dynamics imbalance and mitochondrial dysfunction.
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http://dx.doi.org/10.7717/peerj.7209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611452PMC
July 2019

Behavioral heterogeneity in quorum sensing can stabilize social cooperation in microbial populations.

BMC Biol 2019 03 6;17(1):20. Epub 2019 Mar 6.

Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, Chengdu University, No. 168, Huaguan Road, Chengdu, 610052, Sichuan, China.

Background: Microbial communities are susceptible to the public goods dilemma, whereby individuals can gain an advantage within a group by utilizing, but not sharing the cost of producing, public goods. In bacteria, the development of quorum sensing (QS) can establish a cooperation system in a population by coordinating the production of costly and sharable extracellular products (public goods). Cooperators with intact QS system and robust ability in producing public goods are vulnerable to being undermined by QS-deficient defectors that escape from QS but benefit from the cooperation of others. Although microorganisms have evolved several mechanisms to resist cheating invasion in the public goods game, it is not clear why cooperators frequently coexist with defectors and how they form a relatively stable equilibrium during evolution.

Results: We show that in Pseudomonas aeruginosa, QS-directed social cooperation can select a conditional defection strategy prior to the emergence of QS-mutant defectors, depending on resource availability. Conditional defectors represent a QS-inactive state of wild type (cooperator) individual and can invade QS-activated cooperators by adopting a cheating strategy, and then revert to cooperating when there are abundant nutrient supplies irrespective of the exploitation of QS-mutant defector. Our mathematical modeling further demonstrates that the incorporation of conditional defection strategy into the framework of iterated public goods game with sound punishment mechanism can lead to the coexistence of cooperator, conditional defector, and defector in a rock-paper-scissors dynamics.

Conclusions: These findings highlight the importance of behavioral heterogeneity in stabilizing the population structure and provide a potential reasonable explanation for the maintenance and evolution of cooperation in microbial communities.
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http://dx.doi.org/10.1186/s12915-019-0639-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6889464PMC
March 2019

Evolutionary dynamics in the public goods games with switching between punishment and exclusion.

Chaos 2018 Oct;28(10):103105

Faculty of Natural Sciences and Mathematics, University of Maribor, Koroška cesta 160, SI-2000 Maribor, Slovenia; Complexity Science Hub Vienna, Josefstädterstraße 39, A-1080 Vienna, Austria.

Pro-social punishment and exclusion are common means to elevate the level of cooperation among unrelated individuals. Indeed, it is worth pointing out that the combined use of these two strategies is quite common across human societies. However, it is still not known how a combined strategy where punishment and exclusion are switched can promote cooperation from the theoretical perspective. In this paper, we thus propose two different switching strategies, namely, peer switching that is based on peer punishment and peer exclusion, and pool switching that is based on pool punishment and pool exclusion. Individuals adopting the switching strategy will punish defectors when their numbers are below a threshold and exclude them otherwise. We study how the two switching strategies influence the evolutionary dynamics in the public goods game. We show that an intermediate value of the threshold leads to a stable coexistence of cooperators, defectors, and players adopting the switching strategy in a well-mixed population, and this regardless of whether the pool-based or the peer-based switching strategy is introduced. Moreover, we show that the pure exclusion strategy alone is able to evoke a limit cycle attractor in the evolutionary dynamics, such that cooperation can coexist with other strategies.
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http://dx.doi.org/10.1063/1.5051422DOI Listing
October 2018

The association between elevated serum uric acid levels and islet β-cell function indexes in newly diagnosed type 2 diabetes mellitus: a cross-sectional study.

PeerJ 2018 9;6:e4515. Epub 2018 Mar 9.

Department of Endocrinology, Zhongnan Hospital of Wuhan University, Wu Han, Hu Bei, China.

Background: Serum uric acid (UA) has been reported as a risk factor for type 2 diabetes mellitus (T2DM). However, whether serum UA is associated with insulin resistance and insulin secretion, and the effect of gender on it in the case of the existed association, both remain undefined.

Methods: A cross-sectional study was designed and performed, which enrolled a total of 403 newly diagnosed T2DM patients (mean age, 50.21 ± 13.34 years (62.5% males)). Clinical characteristics and islet function indexes of all participants were analyzed based on gender-specific tertiles of serum UA levels. In addition, multiple linear regression analysis was conducted to investigate covariates associated with islet function indexes.

Results: The mean levels of serum UA were 331.05 μmol/L (interquartile range (IQR): 60.6, 400.9 μmol/L) and 267.9 μmol/L (IQR: 204.7, 331.9 μmol/L) in men and women, respectively. The values of insulin secretion indexes involving AUCins30/glu30, AUCins120/glu120 and total insulin disposition index (DI120) in females were significantly higher than those in males. Apart from the homeostasis model assessment insulin resistance of men, serum UA was positively associated with insulin secretion and insulin resistance indexes both in men and women. Multivariable linear regression analysis showed serum UA exerted an independent impact on insulin secretion in females, but not on insulin resistance. In males, islet function was simultaneously affected by serum UA age, body mass index (BMI), and serum lipids.

Conclusion: Serum UA harbored a positive correlation with insulin secretion and insulin resistance indexes in newly diagnosed T2DM patients, which was influenced by gender, BMI, serum lipids. Hence, serum UA may be considered as a predictor for islet function in clinical practice.
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http://dx.doi.org/10.7717/peerj.4515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5846453PMC
March 2018

TAp63 is correlated with chronic inflammation in patients with newly diagnosed type 2 diabetes mellitus.

J Diabetes Complications 2018 03 29;32(3):335-341. Epub 2017 Dec 29.

Department of Endocrinology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China. Electronic address:

Aims: To investigate TAp63 expression in patients with type 2 diabetes mellitus (T2DM) and the potential correlations between TAp63 and proinflammatory cytokines production and other clinical parameters.

Methods: Peripheral blood mononuclear cells (PBMCs) and plasma were collected from 72 T2DM (cases) and 72 healthy subjects (controls). Fasting blood glucose (FBG), fasting insulin (FIN) and a blood lipid profile were measured. The homeostasis model assessment (HOMA) was used to estimate insulin resistance (IR). Plasma tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 were determined. PBMCs isolated from healthy subjects were cultured with or without 33.3 mmol/l glucose or 0.5 mmol/l palmitic acid (PA) for 6 h, 24 h, 48 h, and 72 h. The expression of TAp63 at mRNA and protein levels in PBMCs was analyzed using real-time qRT-PCR and western blots, respectively.

Results: TAp63 expression was significantly lower in T2DM patients compared with that of the controls. In addition, TAp63 expression showed a negative correlation with FBG, FIN, HbA1c, HOMA-IR, FFAs, TNF-α, and IL-6 levels. Treatment with 33.3 mmol/l glucose or 0.5 mmol/l PA increased TAp63 expression in the cultured PBMCs.

Conclusions: TAp63 level may be correlated with chronic inflammatory state and perturbed glucose and lipid metabolism in T2DM.
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http://dx.doi.org/10.1016/j.jdiacomp.2017.12.013DOI Listing
March 2018

The Combination of the Tumor Markers Suggests the Histological Diagnosis of Lung Cancer.

Biomed Res Int 2017 18;2017:2013989. Epub 2017 May 18.

Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao 266003, China.

Tumor markers are beneficial for the diagnosis and therapy monitoring of lung cancer. However, the value of tumor markers in lung cancer histological diagnosis is unknown. In this study, we analyzed the serum levels of six tumor markers (CEA, CYFRA21-1, SCC, NSE, ProGRP, and CA125) in 2097 suspected patients with lung cancer and determined whether the combination of the tumor markers was useful for histological diagnosis of lung cancer. We found that CYFRA21-1 was the most sensitive marker in NSCLC. ProGRP showed a better clinical performance than that of NSE in discriminating between SCLC and NSCLC. The serum level of CYFRA21-1 or SCC was significantly higher in squamous carcinoma ( < 0.05), and the levels of ProGRP and NSE were significantly higher in SCLC ( < 0.05). According to the criteria established, SCLC and NSCLC were discriminated with sensitivity of 87.12 and 62.63% and specificity of 64.61 and 99.5%, respectively. The sensitivity and specificity in the differentiation of adenocarcinoma and squamous carcinoma were 68.1 and 81.63% and 70.73 and 65.93%, with NPV of 46.03 and 68.97% and PPV of 85.82 and 79.47%, respectively. Our results suggested the combination of six tumor markers could discriminate the histological types of lung cancer.
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http://dx.doi.org/10.1155/2017/2013989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5451759PMC
March 2018

Competitions between prosocial exclusions and punishments in finite populations.

Sci Rep 2017 04 19;7:46634. Epub 2017 Apr 19.

Institute of Technical Physics and Materials Science, Centre for Energy Research, Hungarian Academy of Sciences, P.O. Box 49, H-1525 Budapest, Hungary.

Prosocial punishment has been proved to be a powerful mean to promote cooperation. Recent studies have found that social exclusion, which indeed can be regarded as a kind of punishment, can also support cooperation. However, if prosocial punishment and exclusion are both present, it is still unclear which strategy is more advantageous to curb free-riders. Here we first study the direct competition between different types of punishment and exclusion. We find that pool (peer) exclusion can always outperform pool (peer) punishment both in the optional and in the compulsory public goods game, no matter whether second-order sanctioning is considered or not. Furthermore, peer exclusion does better than pool exclusion both in the optional and in the compulsory game, but the situation is reversed in the presence of second-order exclusion. Finally, we extend the competition among all possible sanctioning strategies and find that peer exclusion can outperform all other strategies in the absence of second-order exclusion and punishment, while pool exclusion prevails when second-order sanctioning is possible. Our results demonstrate that exclusion is a more powerful strategy than punishment for the resolution of social dilemmas.
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http://dx.doi.org/10.1038/srep46634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395949PMC
April 2017

Taxonomic review of the genus Parastenolechia Kanazawa (Lepidoptera, Gelechiidae, Litini) from Mainland China, with descriptions of six new species.

Zootaxa 2016 Oct 20;4178(1):60-78. Epub 2016 Oct 20.

College of Life Sciences, Nankai University, Tianjin 300071, P. R. China; Email:

Ten species of the genus Parastenolechia Kanazawa, 1985 from Mainland China are reviewed. Six species are described as new: Parastenolechia arciformis sp. nov., P. papillaris sp. nov., P. trapezia sp. nov., P. semielliptica sp. nov., P. medispina sp. nov. and P. longifolia sp. nov. Parastenolechia albicapitella Park, 2000 is recorded for the first time in China. A key to the Parastenolechia species that occur in Mainland China is given.
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http://dx.doi.org/10.11646/zootaxa.4178.1.2DOI Listing
October 2016

Review of the genus Aulidiotis Meyrick, 1925 (Lepidoptera: Gelechiidae).

Zootaxa 2016 Jan 4;4061(1):41-50. Epub 2016 Jan 4.

College of Life Sciences, Nankai University, Tianjin 300071, P. R. China; Email: unknown.

The genus Aulidiotis Meyrick, 1925 is reviewed. Aulidiotis trimaculata Li, sp. nov., A. recta Li, sp. nov. and A. biloba Li, sp. nov. are described as new. One possibly new species from Hainan is discussed but not formally named for lack of material. Aulidiotis bicolor Moriuti, 1977 is recorded for the first time in China, and A. phoxopterella (Snellen, 1903) is redescribed. Photographs of adults and genitalia are provided, along with a key to all the species of the genus and a map to exhibit the distribution of these species.
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http://dx.doi.org/10.11646/zootaxa.4061.1.4DOI Listing
January 2016

Melittin-MIL-2 fusion protein as a candidate for cancer immunotherapy.

J Transl Med 2016 06 1;14(1):155. Epub 2016 Jun 1.

Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China.

Background: Cytokine fusion protein that modulates the immune response holds great potential for cancer immunotherapy. IL-2 is an effective treatment against advanced cancers. However, the therapeutic efficacy of IL-2 is limited by severe systemic toxicity. Several mutants recombinant IL-2 can increase antitumor activity and minimize systemic toxicity. Melittin is an attractive anticancer candidate because of its wide-spectrum lytic properties. We previously generated a bifunctional fusion protein melittin-MIL-2, composed of melittin and a mutant IL-2. The melittin-MIL-2 inhibited the growth of human ovarian cancer SKOV3 cells in vitro and in vivo tumor growth. However, whether this antitumor effect could also be used in cancer immunotherapy was unknown. To assess its cancer immunotherapy potential, we further investigated its more effective antitumor immune response and antitumor effect against cancers of different tissue origins in vitro and in vivo.

Methods: The specific IL-2 activity of the melittin-MIL-2 fusion protein was tested on the cytokine growth dependent cell line CTLL-2. The cytolytic activity was detected by standard 4-h (51)Cr-release assays. PBMC stimulation in response to the melittin-MIL-2 was determined by IFN-γ release assay. We observed the cancer cell proliferation of different tissue origins by MTT assay. The ability of melittin-MIL-2 to inhibit tumor growth in vivo was evaluated by using human liver (SMMC-7721 cancer cells), lung (A549 cancer cells) and ovarian (SKOV3 cancer cells) cancer xenograft models. To assess the immunity within the tumor microenvironment, the level of some cytokines including IFN-γ, TNF-α, IL-12 and IL-4 was analyzed by ELISA. We injected the MDA-MB-231 cells and the melittin-MIL-2 into mice, and the anti-metastatic effect was examined by counting nodules in the lung.

Results: The melittin-MIL-2 was more effective in inducing T cell and NK-cell cytotoxicity. The fusion protein significantly increased IFN-γ production in PBMCs. In vitro, the melittin-MIL-2 mediated immune cells killing or directly killed the cancer cell lines of different tissue origins. In vivo, the fusion protein exhibited stronger inhibition on the growth of transplanted human tumors compared to rIL-2. The melittin-MIL-2 treatment promoted the IFN-γ secretion in tumor tissues and decreased the immunosuppressive cells in vivo. Furthermore, the fusion protein reduced lung metastasis of breast cancer.

Conclusions: This study provides the evidence that the melittin-MIL-2 can produce stronger immune stimulation and antitumor effects, and the fusion protein is a potent candidate for cancer immunotherapy.
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http://dx.doi.org/10.1186/s12967-016-0910-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4888606PMC
June 2016

Taxonomic review of the genus Teliphasa Moore, 1888 from China, with descriptions of four new species (Lepidoptera, Pyralidae, Epipaschiinae).

Zookeys 2016 18(554):119-37. Epub 2016 Jan 18.

College of Life Sciences, Nankai University, Tianjin 300071, P. R. China.

The genus Teliphasa Moore, 1888 from China is reviewed. Nine species are treated, including descriptions of four new species: Teliphasa spinosa Li, sp. n., Teliphasa similalbifusa Li, sp. n., Teliphasa erythrina Li, sp. n., and Teliphasa hamata Li, sp. n. Photographs of adults, wing venation, and both male and female genitalia are provided, along with a key for the identification of all the Chinese species.
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http://dx.doi.org/10.3897/zookeys.554.6177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4740832PMC
February 2016

Electrochemically reduced graphene oxide-based electrochemical sensor for the sensitive determination of ferulic acid in A. sinensis and biological samples.

Mater Sci Eng C Mater Biol Appl 2014 Sep 27;42:227-33. Epub 2014 May 27.

Gansu College of Tradition Chinese Medicine, Lanzhou 730000, P.R. China. Electronic address:

An electrochemically reduced graphene oxide (ERGO) modified glassy carbon electrode (GCE) was used as a new voltammetric sensor for the determination of ferulic acid (FA). The morphology and microstructure of the modified electrodes were characterized by scanning electron microscopy (SEM) and Raman spectroscopy analysis, and the electrochemical effective surface areas of the modified electrodes were also calculated by chronocoulometry method. Sensing properties of the electrochemical sensor were investigated by means of cyclic voltammetry (CV) and differential pulse voltammetry (DPV). It was found that ERGO was electrodeposited on the surface of GCE by using potentiostatic method. The proposed electrode exhibited electrocatalytic activity to the redox of FA because of excellent electrochemical properties of ERGO. The transfer electron number (n), electrode reaction rate constant (ks) and electron-transfer coefficient (α) were calculated as 1.12, 1.24s(-1), and 0.40, respectively. Under the optimized conditions, the oxidation peak current was proportional to FA concentration at 8.49 × 10(-8)mol L(-1) to 3.89 × 10(-5)mol L(-1) with detection limit of 2.06 × 10(-8)mol L(-1). This fabricated sensor also displayed acceptable reproducibility, long-term stability, and high selectivity with negligible interferences from common interfering species. The voltammetric sensor was successfully applied to detect FA in A. sinensis and biological samples with recovery values in the range of 99.91%-101.91%.
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http://dx.doi.org/10.1016/j.msec.2014.05.045DOI Listing
September 2014

Optimization of selenylation conditions for a pectic polysaccharide and its structural characteristic.

Int J Biol Macromol 2014 Aug 26;69:244-51. Epub 2014 May 26.

School of Pharmacy, Lanzhou University, Lanzhou 730000, China.

CPP1b is a pectic polysaccharide isolated from Codonopsis pilosula, and it possesses potent antitumor activity. In this study, a HNO3-Na2SeO3 method was performed to synthesize selenium-CPP1b (sCPP1b). The effects of BaCl2 usage amount and HNO3 concentration on the yield and selenium content of sCPP1b were investigated by single-factor experiments. Reaction time, reaction temperature and the ratio of polysaccharide to Na2SeO3 were optimized by orthogonal experimental design at three levels of each of the three factors (L9(3)(4)) based on antitumor activity, selenium content and yield of sCPP1b. Our results showed that 5h of reaction time, 60°C of reaction temperature, and 2:2 ratio of polysaccharide to Na2SeO3 were the optimal selenylation modification conditions. The validation experiments completed under the optimal conditions gave the mean selenium content and yield of sCPP1b were 478.17μg/g (RSD=5.7%) and 595mg/g (RSD=1.6%), respectively. Selenylation modification can significantly increase the antitumor activity of CPP1b in vitro. The structural characterization of sCPP1b was further characterized by Fourier-transform infrared spectroscopy, thermogravimetric analysis, and high-performance gel permeation chromatography coupled with multi-angle laser light scattering. These structure analysis results indicated that sCPP1b has been successfully selenylation modified with similar structure to polysaccharide of CPP1b.
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http://dx.doi.org/10.1016/j.ijbiomac.2014.05.046DOI Listing
August 2014

Preparation, characterization and in vivo distribution of solid lipid nanoparticles loaded with cisplatin.

Pharmazie 2008 Aug;63(8):593-7

College of Pharmacy, Shenyang Pharmaceutical University, Shenyang, PR China.

An emulsification dispersion-ultrasonication method was employed to prepare solid lipid nanoparticles (SLN) loaded with cisplatin. The conventional antitumor drug cisplatin (CDDP) was incorporated into SLN to obtain a targeted and less toxic drug delivery system. The CDDP-SLNs were spherical and uniform in transmission electron microscopy (TEM) photography. The mean particle size and zeta potential were 121 +/- 15 nm, and -46.4 +/- 10.3 mV, respectively. Also, a novel cation exchange resin method was adopted to investigate the encapsulation efficiency (EE%) of the SLN. This method is based on the principle of cation exchange between drugs and resins, and the EE% of the optimal formulation was 82.3%. The in vitro release profile revealed that CDDP was released from SLN efficiently and completely in normal saline (NS) compared with other release media. A pre-column derivatization HPLC method was established for in vivo assay of cisplatin. A tissue distribution study was conducted in male rats after iv administration of 8 mg mL(-1) CDDP-SLN and cisplatin NS, and it was found that CDDP-SLN has a targeted effect to the liver as well as a low concentration in the kidney in rats. These results indicated that emulsification dispersion-ultrasonication is a simple, easy, available and effective method for preparing CDDP-SLN, and the cation exchange resin method is a feasible and suitable method to evaluate the EE% of CDDP-SLN. CDDP-SLN prepared by this method was proved to be a targeted and less toxic drug delivery system.
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August 2008

Development of a benzene vapour sensor utilizing chemiluminescence on Y2O3.

Luminescence 2008 May-Jun;23(3):163-8

Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou 363000, People's Republic of China.

Chemiluminescence (CL) was observed when benzene vapour passed through the surface of Y(2)O(3), TiO(2), Y(2)O(3)-V(2)O(5), TiO(2)-Y(2)O(3), Y(2)O(3)-Cr(2)O(3), Y(2)O(3)-Al(2)O(3) and TiO(2)-Al(2)O(3), with air as the carrier gas. The strongest CL intensity was found with Y(2)O(3) as the catalyst. A novel benzene sensor based on this kind of CL was developed. Quantitative analysis was performed at the wavelength of 425 nm. Under optimal conditions, CL intensity was directly proportional to the concentration of benzene vapour. The linear range was 4-7018 mg/m(3)(r = 0.9981, n = 11), with a detection limit of 1 mg/m(3) (the signal:noise ratio was 3). This gas sensor can work continuously for >80 h and has been successfully applied to the real-time determination of benzene vapour.
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http://dx.doi.org/10.1002/bio.1027DOI Listing
July 2008