Publications by authors named "Lingxia Xu"

9 Publications

  • Page 1 of 1

Toujie Quwen granule used with conventional western therapy for coronavirus disease 2019: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Jun;100(24):e26370

Academician Workstation, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, China.

Background: Coronavirus disease 2019 (COVID-19) is an epidemic infectious disease resulted from 2019 novel coronavirus (2019-nCoV). Up till now, COVID-19 has swept globally. Currently, due to many high-profiled benefits, clinical studies on Toujie Quwen granule (TJQW) have been increasing. The aim of the study is to assess the efficacy and safety of TJQW used with conventional western therapy for COVID-19.

Methods: Relevant randomized controlled trials (RCTs) were searched in Chinese and English databases, and the search time is January 2020 to May 2021. English databases include PubMed, Embase, Web of Science, and the Cochrane Library. Chinese databases include CNKI, WF, VIP, and CBM. The international clinical trial registration platform and the Chinese clinical trial registration platform of controlled trials will be searched by us from January 2020 to May 2021. According to the inclusion and exclusion criteria, screening literature, extraction data will be conducted by 2 researchers independently. Statistical analysis will be conducted using the RevMan 5.3.5 software. After screening the literature based on the inclusion and exclusion criteria, The Recommendation, Assessment, Development, and Evaluation (GRADE) system will be used to evaluate the quality of each result.

Results: This study will provide the evidence for TJQW to be used with conventional western therapy for COVID-19.

Conclusion: The efficacy and safety of TJQW used with conventional western therapy for COVID-19 will be assessed.

Inplasy Registration Number: INPLASY202150038.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000026370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213269PMC
June 2021

Analysis of effect of high-quality nursing on pain of emergency orthopedic trauma patients and related factors affecting postoperative pain.

Am J Transl Res 2021 15;13(4):3658-3665. Epub 2021 Apr 15.

The First Department of Orthopedics, Sanmen People's Hospital Taizhou, Zhejiang Province, China.

Objective: The purpose of this study is to analyze the influencing factors of postoperative pain in patients presenting with emergency orthopedic trauma, and to determine the effect of high-quality nursing on pain.

Methods: In this prospective study, 78 emergency orthopedic trauma patients were randomized into the research group for high-quality nursing and the control group for routine emergency nursing. The two groups were compared with respect to pain degree, pain resolution time, related clinical indexes, pre- and post-treatment joint range of motion (ROM) and psychological state, as well as nursing satisfaction and complications. Univariate analysis and Logistic regression were carried out to analyze the influencing factors of pain following fractures.

Results: Compared with before operation (at baseline), the visual analogue scale (VAS) scores decreased gradually from 1 to 7 days postoperatively in both groups, and were lower in the research group than in the control group in the same time period (all P<0.05). Logistic regression analysis revealed that male, anxiety and/or depression as well as general family support were the major factors affecting postoperative pain in patients with traumatic fractures (P<0.05). Patients in the research group showed significantly less pain resolution time and evidently shorter time of hospitalization and fracture healing than the control group (all P<0.01). Compared with the baseline, ROM scores increased significantly in both groups after intervention, and were higher in the research group than in the control group; Hamilton anxiety (HAMA) and Hamilton depression (HAMD) scores reduced remarkably in the two series, and were even lower in the research group (all P<0.05). In comparison with the control group, the nursing satisfaction was higher and the incidence of complications was lower in the research group (both P<0.05).

Conclusions: Gender, psychological state and family supportive attitudes have certain effects on postoperative pain degree of emergency orthopedic trauma patients. High-quality nursing can relieve postoperative pain degree and adverse psychological state of patients, with fewer complications, higher satisfaction and shorter hospitalization time.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129256PMC
April 2021

Moxibustion for stable chronic obstructive pulmonary disease: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Apr;100(17):e25713

Institute of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Jiangxi, China.

Background: There is no optimal treatment to alleviate the decline of lung function in the stable phase of chronic obstructive pulmonary disease (COPD). The effectiveness of moxibustion as an adjunctive treatment for COPD in the stable phase has been reported clinically, but the conclusions on efficacy and safety have not been unified. This study will systematically evaluate the efficacy and safety of moxibustion on the treatment of COPD in the stable phase, providing clinical-based evidence.

Methods: We will systematically search 7 literature databases and 2 clinical trial registration platforms. The searching time will be conducted from the establishment of databases to March 31, 2021, regardless of language. We will include the randomized controlled trial (RCT) evaluation of moxibustion combined with basic therapy vs basic therapy alone for the treatment of stable COPD. We will assess the risk of bias for individual RCTs using the Cochrane Handbook 5.1.0 evaluation tool. The primary outcome is forced expiratory volume in 1 second/forced vital capacity. The secondary outcomes include forced expiratory volume in 1 second, forced vital capacity, six-minute walking distance, COPD assessment test score, maximum ventilation, response to treatment, and incidence of adverse events. We will collect the effective data of individual RCT through systematic analysis of the random effect model. Heterogeneity will be tested by Cochran Q test and I-squared statistics. Two subgroup analyses will be performed to explore the sources of heterogeneity based on clinical experience. Excluding RCTs with a high risk of bias, fixed-effect model will be used for sensitivity analysis to test the robustness of the meta-analysis results. The publication bias will be assessed by funnel plot and Egger test.

Results: This study will provide systematic evidence on the efficacy and safety of moxibustion on the treatment of patients with stable COPD through strict quality assessment and reasonable data synthesis. We hope that the results will be submitted to a peer-reviewed journal for publication.

Conclusion: This systematic review will provide the best current evidence for the adjuvant treatment of stable COPD with moxibustion.

Inplasy Registration Number: INPLASY202140047.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000025713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084073PMC
April 2021

The Relationship between Hepatic Myeloid-Derived Suppressor Cells and Clinicopathological Parameters in Patients with Chronic Liver Disease.

Biomed Res Int 2021 29;2021:6612477. Epub 2021 Mar 29.

Department of Traditional Chinese Medicine, School of Medicine, Xiamen University, Xiamen, Fujian, China.

Myeloid-derived suppressor cells (MDSCs) have attracted attention due to their important role in inflammation. Several studies have investigated the involvement of MDSCs in chronic liver disease. However, due to the difference of MDSC phenotypes, patient types, and sample sources among the studies, the results are inconsistent and controversial. We took advantage of a large well-defined cohort of 98 (24 patients with CHB, 18 with NAFLD, 13 with HCC, 16 with PBC, and 27 with AIH) patients with liver inflammation and 12 healthy controls to investigate the expression of MDSCs, and the relationships between the expression of hepatic MDSCs and the clinical characteristics were analyzed. We found that the expression of CD11b+CD33+ MDSCs is closely related to chronic liver disease and positively correlated with clinical parameters such as ALT, AST, and globulin. Ultimately, the present study suggests that hepatic CD11b+CD33+ MDSCs are increased in HCC and AIH and positively correlate with the liver stages of hepatitis activity and liver fibrosis stage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6612477DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024072PMC
May 2021

Therapeutic Effects of (5R)-5-Hydroxytriptolide on Fibroblast-Like Synoviocytes in Rheumatoid Arthritis lncRNA WAKMAR2/miR-4478/E2F1/p53 Axis.

Front Immunol 2021 16;12:605616. Epub 2021 Feb 16.

Department of Rheumatology, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai, China.

Rheumatoid arthritis (RA) is an autoimmune disease. Fibroblast-like synoviocytes (FLS) serve a major role in synovial hyperplasia and inflammation in RA. (5R)-5-hydroxytriptolide (LLDT-8), a novel triptolide derivative, shows promising therapeutic effects for RA and is now in phase II clinical trials in China. However, the underlying mechanism of LLDT-8 is still not fully understood. Here, we found that LLDT-8 inhibited proliferation and invasion of RA FLS, as well as the production of cytokines. Microarray data demonstrated that LLDT-8 upregulated the expression of long non-coding RNA (lncRNA) WAKMAR2, which was negatively associated with proliferation and invasion of RA FLS, as well as the production of pro-inflammatory cytokines. Knockdown of WAKMAR2 abolished the inhibitory effects of LLDT-8 on RA FLS. Mechanistically, WAKMAR2 sponged miR-4478, which targeted E2F1 and downstreamed p53 signaling. Rescue experiments indicated that the inhibitory effects of LLDT-8 on RA FLS were dependent on WAKMAR2/miR-4478/E2F1/p53 axis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.605616DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921149PMC
June 2021

Epigenetic Regulation Mediated by Methylation in the Pathogenesis and Precision Medicine of Rheumatoid Arthritis.

Front Genet 2020 4;11:811. Epub 2020 Aug 4.

Department of Rheumatology, Guanghua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Rheumatoid arthritis (RA) is a complex disease triggered by the interaction between genetics and the environment, especially through the shared epitope (SE) and cell surface calreticulin (CSC) theory. However, the available evidence shows that genetic diversity and environmental exposure cannot explain all the clinical characteristics and heterogeneity of RA. In contrast, recent studies demonstrate that epigenetics play important roles in the pathogenesis of RA, especially DNA methylation and histone modification. DNA methylation and histone methylation are involved in innate and adaptive immune cell differentiation and migration, proliferation, apoptosis, and mesenchymal characteristics of fibroblast-like synoviocytes (FLS). Epigenetic-mediated regulation of immune-related genes and inflammation pathways explains the dynamic expression network of RA. In this review, we summarize the comprehensive evidence to show that methylation of DNA and histones is significantly involved in the pathogenesis of RA and could be applied as a promising biomarker in the disease progression and drug-response prediction. We also explain the advantages and challenges of the current epigenetics research in RA. In summary, epigenetic modules provide a possible interface through which genetic and environmental risk factors connect to contribute to the susceptibility and pathogenesis of RA. Additionally, epigenetic regulators provide promising drug targets to develop novel therapeutic drugs for RA. Finally, DNA methylation and histone modifications could be important features for providing a better RA subtype identification to accelerate personalized treatment and precision medicine.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2020.00811DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417338PMC
August 2020

Impact of Hepatic Steatosis on the Antiviral Effects of PEG-IFN-2a in Patients with Chronic Hepatitis B and the Associated Mechanism.

Gastroenterol Res Pract 2020 26;2020:1794769. Epub 2020 Jun 26.

Department of Traditional Chinese Medicine, Medical College of Xiamen University, Xiamen, Fujian, China.

Objective: To investigate the risk factors for hepatic steatosis in chronic hepatitis B (CHB), to determine its correlation with liver necroinflammation and fibrosis and response to peginterferon alpha-2a (PEG-IFN-2a) antiviral therapy, and to explore the mechanisms underlying the poor antiviral effect of PEG-IFN-2a in CHB patients with hepatic steatosis.

Methods: We analysed the impact of hepatic steatosis on the antiviral effect of PEG-IFN-2a on CHB patients in a cohort of 226 patients who underwent pretherapeutic liver biopsy. To assess the complete response (CR), virological response (VR), and biochemical response (BR), the 226 patients were treated with PEG-IFN-2a for 48 weeks and were followed-up for 24 weeks. The expressions of hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg) in the liver tissue were detected in all patients to explore the possible mechanism of hepatic steatosis with regard to antiviral effects.

Results: The patients were divided into four groups based on the severity of hepatic steatosis: 119 with no steatosis, 76 with mild steatosis, 22 with moderate steatosis, and 9 with severe steatosis. In the hepatic steatosis groups, the proportions of male patients, patients aged >40 years, patients with hyperuricaemia, patients with a BMI > 23 kg/m, and total cholesterol (TC), triglyceride (TG), glucose (GLU), and uric acid (UA) levels were significantly higher than those in the group without steatosis, whereas the alanine aminotransferase (ALT) and aspartate transaminase (AST) levels were significantly lower than those in the group without steatosis. The multivariate analysis results indicated that a BMI > 23 kg/m was independently associated with CHB patients with hepatic steatosis; the levels of baseline AST and UA were independently associated with CHB patients with significant hepatic steatosis, and the baseline AST level was independently associated with significant liver fibrosis. After 48 weeks of treatment and 24 weeks of follow-up, the rates of CR, VR, and BR had gradually decreased, whereas the severity of hepatic steatosis had increased.

Conclusion: Hepatic steatosis can reduce the efficacy of PEG-IFN-2a in the treatment of CHB patients, and its mechanism may be related to the different HBcAg expression patterns in liver tissue.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/1794769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335408PMC
June 2020

Effect of particle size on the stability and flavor of cloudy apple juice.

Food Chem 2020 Oct 4;328:126967. Epub 2020 May 4.

College of Food Science and Technology, Bohai University, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, Liaoning 121013, China. Electronic address:

Different particle sizes in cloudy apple juice were obtained following filtration with different mesh sizes (100, 200, 300, and 400-mesh). The effects of cloud particle size on the stability, nutrient content, and volatile flavor of cloudy apple juice were evaluated. With increasing mesh number, particle size decreased (p < 0.05) and particle shape changed. Particle size had an effect on volatile flavor compounds, especially nitrogen oxides, alcohols, and aromatic compounds. The content of pectin and total phenol decreased with decreasing particle size, while the content of soluble protein was not affected. The reduction of cloud particle size increased absolute value of ζ-potential, cloud stability, and apparent viscosity and decreased turbidity and cloud values. Pearson correlation analysis showed that there was a strong correlation between particle size and quality indicators, except for soluble protein.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2020.126967DOI Listing
October 2020

[Whole-genome sequencing and analysis of a adrostenedione yielding strains Mycobacterium neoaurum MN4].

Wei Sheng Wu Xue Bao 2016 Aug;56(8):1358-67

Objective: Mycobaterium neoaurum MN4 is a substrate-resistant mutant strain with high-yield androstenedione. In order to further study MN4 strain substrate-resistant mechanism and androstenedione biosynthetic pathway, it is necessary to decipher the MN4 strain genome.

Methods: The genome was sequenced using highthroughput sequencing technology, and analyzed using relevant software for genome assembly, gene prediction and functional annotation, COG cluster analysis and secondary metabolite biosynthesis gene clusters prediction.

Results: The whole genome is assembled into 33 contigs, and the genome size is 5.39 Mb, GC content of 66.9% with encoding 4920 protein genes. The genome sequence was deposited in the GenBank database under the accession number JXYZ00000000.

Conclusion: This study is the first report of androstenedione producing strain Mycobacterium neoaurum MN4 genome sequence, and provides a theoretical basis for further heterologous expression of secondary metabolites on Mycobacterium neoaurum MN4.
View Article and Find Full Text PDF

Download full-text PDF

Source
August 2016
-->