Publications by authors named "Lingling Zhang"

670 Publications

mTORC2 facilitates liver regeneration through the sphingolipid-induced PPAR-α-fatty acid oxidation.

Cell Mol Gastroenterol Hepatol 2022 Aug 2. Epub 2022 Aug 2.

Key Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou, 510632, China. Electronic address:

Background & Aims: During liver regeneration following partial hepatectomy, the function and metabolic pathways governing transient lipid droplet accumulation in hepatocytes remain obscure. Mammalian target of rapamycin 2 (mTORC2) facilitates de novo synthesis of hepatic lipids. Under normal conditions and in tumorigenesis, decreased levels of triglyceride (TG) and fatty acids (FAs) are observed in the mTORC2-deficient liver. However, during liver regeneration, their levels increase in the absence of mTORC2.

Methods: Rictor liver-specific knockout (R-LKO) and control mice underwent partial hepatectomy, followed by measurement of TG and FA contents during liver regeneration. FA metabolism was evaluated by analyzing the expression of FA metabolism-related genes and proteins. Intraperitoneal injection of the peroxisome proliferator-activated receptor α (PPAR-α) agonist, p53 inhibitor, and AKT activator was performed to verify the regulatory pathways involved. Lipid mass spectrometry was performed to identify the potential PPAR-α activators.

Results: The expression of FA metabolism-related genes and proteins suggested that FAs are mainly transported into hepatocytes during liver regeneration. The PPAR-α pathway is significantly downregulated in the mTORC2-deficient liver, resulting in the accumulation of TGs. The PPAR-α agonist WY-14643 rescued deficient liver regeneration and survival in mTORC2-deficient mice. Furthermore, lipidomic analysis suggested that mTORC2 deficiency substantially reduced glucosylceramide (GluCer) content. GluCer activated PPAR-α. GluCer treatment in vivo restored the regenerative ability and survival rates in the mTORC2-deficient group.

Conclusions: Our data suggest that FAs are mainly transported into hepatocytes during liver regeneration, and their metabolism is facilitated by mTORC2 through the GluCer-PPAR-α pathway, thereby establishing a novel role for mTORC2 in lipid metabolism.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcmgh.2022.07.011DOI Listing
August 2022

Advance in bone destruction participated by JAK/STAT in rheumatoid arthritis and therapeutic effect of JAK/STAT inhibitors.

Int Immunopharmacol 2022 Aug 1;111:109095. Epub 2022 Aug 1.

Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-Inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Centre of Anti-Inflammatory and Immune Medicine, Center of Rheumatoid Arthritis of Anhui Medical University, Hefei 230032, China. Electronic address:

Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic joint inflammation and bone erosion. The bones in the human body are constantly undergoing bone remodeling throughout their lives, which is the process of bone resorption by osteoclasts to damaged bone tissue and new bone formation by osteoblasts. Osteoblasts (OBs) are the main functional cells in bone formation, responsible for the synthesis, secretion and mineralization of the bone matrix. On the contrary, osteoclasts (OCs) mediate bone breakdown during natural bone turnover, but excessive breakdown occurs in RA. Under the condition of RA inflammation, many molecules, such as IL-1β, IL-6, TNF-α, IL-17 and hypoxia-inducible factor-1α (HIF-1α) are produced that could mediate bone loss. Studies have shown that cytokines mainly promote the formation of OCs and play a role in bone resorption by stimulating OBs to express receptor activator of NF-κB ligand (RANKL). JAK/STAT plays a crucial role in the process of bone destruction. And JAK/STAT pathway mediates the RANKL/receptor activator of NF-κB (RANK)/osteoprotegerin (OPG) axis. Tofacitinib, Baricitinib, Peficitinib and Filgotinib are now being used in patients with moderate to severe RA, as well as in patients with RA who have an inadequate response to methotrexate therapy and bone destruction. Currently, Tofacitiniband Baritinib areapprovedfor thetreatmentof moderate-to-severely active RA. JAK inhibitors have been reported to have better efficacy and lower adverse effects compared with methotrexate and adalimumab. In addition, two JAK inhibitors are currently in development: the JAK1 selective Upadacitinib, and the JAK3 selective inhibitor Decernotinib. In addition to the above JAK inhibitors, some small molecular compounds inhibit bone destruction by inhibiting the Phosphorylation of STAT3. In this paper, the research progress of bone destruction participated by JAK/ STAT in rheumatoid arthritis and therapeutic effect of JAK/STAT inhibitors were reviewed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.intimp.2022.109095DOI Listing
August 2022

[Design and application of an anti-pull device for medical drainage tube].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2022 Jun;34(6):653-654

Department of Nursing, Cerebrovascular Disease Hospital of Henan Provincial People's Hospital, Henan Provincial Key Laboratory of Nursing Medicine, People's Hospital of Zhengzhou University, Zhengzhou 450003, Henan, China.

The management of drainage tube is an important part of nursing work. Patient restraint and tube fixation cannot effectively prevent unplanned extubation (UEX) when the tube is accidentally pulled by violence. The nursing innovation team of Henan Provincial People's Hospital designed a medical drainage tube anti-pull device in order to change the existing technology of preventing drainage tube disconnecting by means of restraint and fixation, and to interfere with the basic cause of drainage tube disconnection, and obtained the national utility model patent (patent number: ZL 2020 2 2843025.1). The design of sleeve and clasp is that when the drainage tube is pulled by accidental violence, the friction fastener clamps the drainage tube mechanically to achieve the purpose of braking the drainage tube and prevent the drainage tube from coming out. Card sleeve ring fracture design can be applied to drainage tubes of different diameters, and the buzzer device at the instant of the snap ring into the card set warning medical staff to the occurrence of risk events, so that the nurse can come in the first place for effective treatment, which is a fuse for surgical drainage tubes and is to timely and effectively prevent UEX.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3760/cma.j.cn121430-20220329-00311DOI Listing
June 2022

Adaptive bird-like genome miniaturization during the evolution of scallop swimming lifestyle.

Genomics Proteomics Bioinformatics 2022 Jul 26. Epub 2022 Jul 26.

Sars-Fang Centre & MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Biology and Biotechnology, Pilot Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Sanya 572000, China. Electronic address:

Genome miniaturization drives key evolutionary innovations of adaptive traits in vertebrates, such as the flight evolution of birds. However, a similar evolutionary process remains poorly understood in invertebrates. Derived from the second-largest animal phylum, scallops are a special group of bivalve molluscs and acquire the evolutionary novelty of the swimming lifestyle, providing excellent models for investigating the coordinated genome and lifestyle evolution. Here, we show for the first time that genome sizes of scallops exhibit a generally negative correlation with locomotion activity. To elucidate the co-evolution of genome size and swimming lifestyle, we focus on the Asian moon scallop (Amusium pleuronectes) that possesses the smallest known scallop genome while being among scallops with the highest swimming activity. Whole-genome sequencing of A. pleuronectes reveals highly conserved chromosomal macrosynteny and microsynteny, suggestive of a highly contracted but not degenerated genome. Genome reduction of A. pleuronectes is facilitated by significant inactivation of transposable elements, leading to reduced gene length and elevated expression of energy-producing gene pathways and the decrease of copy number and expression level of biomineralization genes. Similar evolutionary changes of relevant pathways are also observed for bird genome reduction with flight evolution. The striking mimicry of genome miniaturization underlying the evolution of bird flight and scallop swimming unveils the potentially common, pivotal role of genome size fluctuation in the evolution of novel lifestyles in the animal kingdom.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gpb.2022.07.001DOI Listing
July 2022

Antibacterial activity and mechanism of ginger extract against Ralstonia solanacearum.

J Appl Microbiol 2022 Jul 26. Epub 2022 Jul 26.

College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei, China.

Aims: The current study aimed to determine the chemical compositions of ginger extract (GE) and to assess the antibacterial activities of GE against the ginger bacterial wilt pathogen Ralstonia solanacearum and to screen their mechanisms of action.

Methods And Results: A total of 393 compounds were identified by using ultra-performance liquid chromatography and tandem-mass spectrometry. The antibacterial test indicated that GE had strong antibacterial activity against R. solanacearum and that the bactericidal effect exhibited a dose-dependent manner. The minimum inhibitory concentration and minimum bactericidal concentration to R. solanacearum was 3.91 mg/mL and 125 mg/mL, respectively. The cell membrane permeability and integrity of R. solanacearum were destroyed by GE, resulting in cell content leakage, such as electrolytes, nucleic acids, proteins, extracellular adenosine triphosphate, and exopoly saccharides. In addition, the activity of cellular succinate dehydrogenase and alkaline phosphatase of R. solanacearum decreased gradually with an increase in the GE concentration. Scanning electron microscopy analysis revealed that GE treatment changed the morphology of the R. solanacearum cells. Further experiments demonstrated that GE delayed or slowed the occurrence of bacterial wilt on ginger.

Conclusions: GE has a significant antibacterial effect on R. solanacearum, and the antibacterial effect is concentration dependent. The GE treatments changed the morphology, destroyed membrane permeability and integrity, reduced key enzyme activity, and inhibit the synthesis of the virulence factor EPS of R. solanacearum. GE significantly controlled the bacterial wilt of ginger during infection.

Significance And Impact Of The Study: This research provides insight into the antimicrobial mechanism of GE against R. solanacearum, which will open a new application field for GE.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jam.15733DOI Listing
July 2022

Readiness for Hospital Discharge and Its Correlation with the Quality of Discharge Teaching among the Parents of Premature Infants in NICU.

Appl Bionics Biomech 2022 13;2022:4924021. Epub 2022 Jul 13.

Health Management Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Objectives: To assess the extent of the readiness for hospital discharge and the correlation with discharge teaching quality among parents of premature infants' in the NICU.

Background: Low readiness for discharge from the hospital can lead to negative outcomes in healthcare for infants born prematurely and their parents. Discharge guidelines are a basic approach to ensure the readiness of the parents for discharge from the hospital. No investigation has ever been conducted into the sufficiency of hospital discharge guidelines for premature infants and their impact on parental readiness for hospital discharge.

Design: Data was collected from four hospitals in China using a correlational descriptive study.

Methods: Two hundred and eight parents of premature NICU-hospitalized infants of four tertiary hospitals in Henan Province from May to October 2020 were enrolled. The general information questionnaire, the readiness for hospital discharge scale- (RHDS-) parent form, and the quality of discharge teaching scale- (QDTS-) parent form were used for data collection. Spearman correlation analysis and descriptive statistics were used to analyze the data.

Results: The total score for hospital discharge readiness was high (8.05 ± 1.11). The total score of the quality of discharge guidelines was moderate (7.44 ± 1.44). Moreover, the discharge teaching quality was positively correlated with the parents' readiness. Positive correlations were found between PRHDS and QDTS subscales, including content received and delivery, physical-emotional status, knowledge, and expected support.

Conclusion: The quality of the discharge guidelines perceived by parents of premature infants was moderate, which may have reduced their readiness for hospital discharge. . This study furnishes basic information on the importance of readiness of discharge for the parents of premature infants. The teaching guides nurses to enhance the quality of discharge teaching and the readiness of parents for discharge from the hospital.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/4924021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300354PMC
July 2022

Function of CTLGA9 Amino Acid Residue Leucine-6 in Modulating Cry Toxicity.

Front Immunol 2022 5;13:906259. Epub 2022 Jul 5.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.

is a crucial vector for many arboviral diseases that cause millions of deaths worldwide and thus is of major public health concern. Crystal (Cry) proteins, which are toxins produced by , are structurally organized into three-domains, of which domain II is the most variable in terms of binding towards various toxin receptors. The binding of Cry11Aa to putative receptor such as aminopeptidase-N (APN) is explicitly inhibited by midgut C-type lectins (CTLs). The similarity between the domain II fold of Cry11Aa toxin and the carbohydrate recognition domain in the CTLs is a possible structural basis for the involvement of Cry domain II in the recognition of carbohydrates on toxin receptors. In this study, a site-directed point mutation was introduced into the CTLGA9 gene on the basis of molecular docking findings, leading to substitution of the Leucine-6 (Leu-6) residue in the protein with alanine. Subsequently, functional monitoring of the mutated protein was carried out. Unlike the amino acid residues of wild-type CTLGA9, none of the residues of mutant () CTLGA9 were competed with Cry11Aa for binding to the APN receptor interface. Additionally, ligand blot analysis showed that both wild-type and mutant CTLGA9 had similar abilities to bind to APN and Cry11Aa. Furthermore, in the competitive ELISA in which labeled mutant CTLGA9 (10 nM) was mixed with increasing concentrations of unlabeled Cry11Aa (0-500 nM), the mutant showed no competition with Cry11Aa for binding to APN., By contrast, in the positive control sample of labeled wild type CTLGA9 mixed with same concentrations of Cry11Aa competition between the two ligands for binding to the APN was evident. These results suggest that Leucine-6 may be the key site involved in the competitive receptor binding between CTLGA9 and Cry11Aa. Moreover, according to the bioassay results, mutant CTLGA9 could in fact enhance the toxicity of Cry11Aa. Our novel findings provide further insights into the mechanism of Cry toxicity as well as a theoretical basis for enhancing the mosquitocidal activity of these toxin through molecular modification strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2022.906259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294448PMC
July 2022

Discovery of a highly specific F-labeled PET ligand for phosphodiesterase 10A enabled by novel spirocyclic iodonium ylide radiofluorination.

Acta Pharm Sin B 2022 Apr 17;12(4):1963-1975. Epub 2021 Nov 17.

Center of Cyclotron and PET Radiopharmaceuticals, Department of Nuclear Medicine and PET/CT-MRI Center, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China.

As a member of cyclic nucleotide phosphodiesterase (PDE) enzyme family, PDE10A is in charge of the degradation of cyclic adenosine (cAMP) and guanosine monophosphates (cGMP). While PDE10A is primarily expressed in the medium spiny neurons of the striatum, it has been implicated in a variety of neurological disorders. Indeed, inhibition of PDE10A has proven to be of potential use for the treatment of central nervous system (CNS) pathologies caused by dysfunction of the basal ganglia-of which the striatum constitutes the largest component. A PDE10A-targeted positron emission tomography (PET) radioligand would enable a better assessment of the pathophysiologic role of PDE10A, as well as confirm the relationship between target occupancy and administrated dose of a given drug candidate, thus accelerating the development of effective PDE10A inhibitors. In this study, we designed and synthesized a novel F-aryl PDE10A PET radioligand, codenamed [F]P10A-1910 ([F]), in high radiochemical yield and molar activity spirocyclic iodonium ylide-mediated radiofluorination. [F] possessed good binding affinity (IC = 2.1 nmol/L) and selectivity towards PDE10A. Further, [F] exhibited reasonable lipophilicity (log = 3.50) and brain permeability (  > 10 × 10 cm/s in MDCK-MDR1 cells). PET imaging studies of [F] revealed high striatal uptake and excellent specificity with reversible tracer kinetics. Preclinical studies in rodents revealed an improved plasma and brain stability of [F] when compared to the current reference standard for PDE10A-targeted PET, [F]MNI659. Further, dose-response experiments with a series of escalating doses of PDE10A inhibitor in rhesus monkey brains confirmed the utility of [F] for evaluating target occupancy in higher species. In conclusion, our results indicated that [F] is a promising PDE10A PET radioligand for clinical translation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.apsb.2021.11.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279629PMC
April 2022

Umbilical cord artery-derived perivascular stem cells for treatment of ovarian failure through CD146 signaling.

Signal Transduct Target Ther 2022 07 13;7(1):223. Epub 2022 Jul 13.

Center for Reproductive Medicine and Obstetrics and Gynecology, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41392-022-01029-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276707PMC
July 2022

Casted MoS nanostructures and their Raman properties.

Nanoscale 2022 Jul 28;14(29):10449-10455. Epub 2022 Jul 28.

Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, China.

Two-dimensional (2D) transition metal dichalcogenides (TMDCs) have been widely investigated for optoelectronic applications. Here, by employing the nanocasting method, molybdenum disulfide (MoS) nanostructures, including supercrystals, nanoparticles and nanowires, are synthesized with curved features by changing the precursor concentration and template types. The Raman properties of different MoS nanostructures are investigated by varying the laser power under both resonant and non-resonant excitations. The defect disorder induced LA() mode and other silent Raman modes in planar 2D materials are clearly observed under the resonant excitation. We believe that the varying optical properties of TMDC nanostructures will greatly broaden the optoelectronic applications of 2D materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2nr02593kDOI Listing
July 2022

Photothermal-Driven High-Performance Selective Hydrogenation System Enabled by Delicately Designed IrCo Nanocages.

Small 2022 Jul 20;18(29):e2201271. Epub 2022 Jun 20.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.

The incorporation of a transition metal into a noble metal for the formation of nanoalloys paves a potential way to modulate the electronic structures and spatial arrangement modes, thereby manipulating the target catalysis under the desired reaction pathways. Herein, a top-down synthetic route to fabricate IrCo nanoalloys with delicately designed compositions and morphologies at an extremely low calcination temperature of 200 °C is reported, which efficiently breaks through the thermodynamic limitations caused by the large atomic radii and electronegativity discrepancies between Co and Ir. A high-performance selective hydrogenation system enabled by the synthesized IrCo nanoalloys and the light irradiation is further established. Significantly, the unique properties of IrCo alloy, involving the special capability of generating local heating rather than hot electrons under light irradiation (the hot-electron effect was considered detrimental to hydrogenation reactions), as well as the highly polarized surface which aids in the hydrogen transfer from borane-ammonia complex (AB) to 4-nitrostyrene (4-NS) are discovered.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202201271DOI Listing
July 2022

Inhibition of AKT induces p53/SIRT6/PARP1-dependent parthanatos to suppress tumor growth.

Cell Commun Signal 2022 06 17;20(1):93. Epub 2022 Jun 17.

Department of Health Management, The Third Xiangya Hospital, Central South University, Changsha, China.

Background: Targeting AKT suppresses tumor growth through inducing apoptosis, however, during which whether other forms of cell death occurring is poorly understood.

Methods: The effects of increasing PARP1 dependent cell death (parthanatos) induced by inhibiting AKT on cell proliferation were determined by CCK-8 assay, colony formation assay, Hoechst 33,258 staining and analysis of apoptotic cells by flow cytometry. For the detailed mechanisms during this process, Western blot analysis, qRT-PCR analysis, immunofluorescence and co-immunoprecipitation were performed. Moreover, the inhibition of tumor growth by inducing p53/SIRT6/PARP1-dependent parthanatos was further verified in the xenograft mouse model.

Results: For the first time, we identified that inhibiting AKT triggered parthanatos, a new form of regulated cell death, leading to colon cancer growth suppression. For the mechanism investigation, we found that after pharmacological or genetic AKT inhibition, p53 interacted with SIRT6 and PARP1 directly to activate it, and promoted the formation of PAR polymer. Subsequently, PAR polymer transported to outer membrane of mitochondria and resulted in AIF releasing and translocating to nucleus thus promoting cell death. While, blocking PARP1 activity significantly rescued colon cancer from death. Furthermore, p53 deletion or mutation eliminated PAR polymer formation, AIF translocation, and PARP1 dependent cell death, which was promoted by overexpression of SIRT6. Meanwhile, reactive oxygen species production was elevated after inhibition of AKT, which might also play a role in the occurrence of parthanatos. In addition, inhibiting AKT initiated protective autophagy simultaneously, which advanced tumor survival and growth.

Conclusion: Our findings demonstrated that AKT inhibition induced p53-SIRT6-PARP1 complex formation and the activation of parthanatos, which can be recognized as a novel potential therapeutic strategy for cancer. Video Abstract.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12964-022-00897-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205131PMC
June 2022

Nanoporous biochar with high specific surface area based on rice straw digestion residue for efficient adsorption of mercury ion from water.

Bioresour Technol 2022 Sep 13;359:127471. Epub 2022 Jun 13.

College of Engineering, Northeast Agricultural University, Harbin 150030, China; College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China. Electronic address:

The unreasonable disposal of residue after anaerobic digestion seriously affects the stability of the ecosystem, and the preparation of adsorbent is an effective way to value-added utilization of the residue. In this study, a high adsorption capacity (209.65 mg/g) biochar-based adsorbent was prepared by hydrothermal carbonization and alkali modification using rice straw biogas residue. The lignocellulosic structure was destroyed after anaerobic digestion, forming porous biochar with larger specific surface area (2372.51 m/g) and richer pore structure. Besides, the mercury ion complexed on the adsorbent surface in monovalent and divalent forms and possessed favorable selectivity in the presence of other examples of interference. The adsorption process is consistent with pseudo second-order kinetics and the Langmuir isotherm, indicating a predominance of chemisorption. This study provides a methodology for use of rice straw biogas residue and treatment of mercury containing wastewater, which offers a fresh direction for resource utilization of biogas residue.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2022.127471DOI Listing
September 2022

TN-ZSTAD: Transferable Network for Zero-Shot Temporal Activity Detection.

IEEE Trans Pattern Anal Mach Intell 2022 Jun 16;PP. Epub 2022 Jun 16.

An integral part of video analysis and surveillance is temporal activity detection, which means to simultaneously recognize and localize activities in long untrimmed videos. Currently, the most effective methods of temporal activity detection are based on deep learning, and they typically perform very well with large scale annotated videos for training. However, these methods are limited in real applications due to the unavailable videos about certain activity classes and the time-consuming data annotation. To solve this challenging problem, we propose a novel task setting called zero-shot temporal activity detection (ZSTAD), where activities that have never been seen in training still need to be detected. We design an end-to-end deep transferable network TN-ZSTAD as the architecture for this solution. On the one hand, this network utilizes an activity graph transformer to predict a set of activity instances that appear in the video, rather than produces many activity proposals in advance. On the other hand, this network captures the common semantics of seen and unseen activities from their corresponding label embeddings, and it is optimized with an innovative loss function that considers the classification property on seen activities and the transfer property on unseen activities together. Experiments on the THUMOS'14, Charades, and ActivityNet datasets show promising performance in terms of detecting unseen activities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TPAMI.2022.3183586DOI Listing
June 2022

Decoding the byssus fabrication by spatiotemporal secretome analysis of scallop foot.

Comput Struct Biotechnol J 2022 27;20:2713-2722. Epub 2022 May 27.

Sars-Fang Centre & MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.

Secretome is involved in almost all physiological, developmental, and pathological processes, but to date there is still a lack of highly-efficient research strategy to comprehensively study the secretome of invertebrates. Adhesive secretion is a ubiquitous and essential physiological process in aquatic invertebrates with complicated protein components and unresolved adhesion mechanisms, making it a good subject for secretome profiling studies. Here we proposed a computational pipeline for systematic profiling of byssal secretome based on spatiotemporal transcriptomes of scallop. A total of 186 byssus-related proteins (BRPs) were identified, which represented the first characterized secretome of scallop byssal adhesion. Scallop byssal secretome covered almost all of the known structural elements and functional domains of aquatic adhesives, which suggested this secretome-profiling strategy had both high efficiency and accuracy. We revealed the main components of scallop byssus (including EGF-like domain containing proteins, the Tyr-rich proteins and 4C-repeats containing proteins) and the related modification enzymes primarily contributing to the rapid byssus assembly and adhesion. Spatiotemporal expression and co-expression network analyses of BRPs suggested a simultaneous secretion pattern of scallop byssal proteins across the entire region of foot and revealed their diverse functions on byssus secretion. In contrast to the previously proposed "root-initiated secretion and extension-based assembly" model, our findings supported a novel "foot-wide simultaneous secretion and in situ assembly" model of scallop byssus secretion and adhesion. Systematic analysis of scallop byssal secretome provides important clues for understanding the aquatic adhesive secretion process, as well as a common framework for studying the secretome of non-model invertebrates.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.csbj.2022.05.048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168380PMC
May 2022

Influence of rigid wall on the nonlinear pulsation of nearby bubble.

Ultrason Sonochem 2022 Jun 13;87:106034. Epub 2022 May 13.

Key Laboratory of Modern Acoustics, Ministry of Education, Institute of Acoustics, Nanjing University, Nanjing 210093, China.

This paper mainly focuses on the nonlinear pulsation of a bubble near the rigid wall. Dynamics of near-wall bubble and free bubble are discussed and compared in details. Investigation reveals as the driving acoustic pressure amplitude increases, nonlinear pulsation of bubble becomes intense gradually. Besides, decreasing the viscosity of host liquid is advantageous for the nonlinear pulsation of bubble. Bifurcation diagrams of bubble radius show acoustic reflection of the rigid wall makes the initial bifurcation appear at low driving acoustic amplitude and on bubble with small ambient radius, and makes the bifurcation still exist for bubble in high-viscosity liquids. That indicates the rigid wall will produce enhancement on the nonlinearity of nearby bubble. As the bubble approaches the wall, the enhancement becomes strong. Moreover, research on the influence of driving frequency shows the rigid wall makes the frequency band corresponding to chaos around the resonant frequency of free bubble shift downward.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ultsonch.2022.106034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160405PMC
June 2022

Role of Lectin in the Response of Against Toxin.

Front Immunol 2022 13;13:898198. Epub 2022 May 13.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.

is one of the world's most dangerous mosquitoes, and a vector of diseases such as dengue fever, chikungunya virus, yellow fever, and Zika virus disease. Currently, a major global challenge is the scarcity of antiviral medicine and vaccine for arboviruses. var israelensis () toxins are used as biological mosquito control agents. Endotoxins, including Cry4Aa, Cry4Ba, Cry10Aa, Cry11Aa, and Cyt1Aa, are toxic to mosquitoes. Insect eradication by Cry toxin relies primarily on the interaction of cry toxins with key toxin receptors, such as aminopeptidase (APN), alkaline phosphatase (ALP), cadherin (CAD), and ATP-binding cassette transporters. The carbohydrate recognition domains (CRDs) of lectins and domains II and III of Cry toxins share similar structural folds, suggesting that midgut proteins, such as C-type lectins (CTLs), may interfere with interactions among Cry toxins and receptors by binding to both and alter Cry toxicity. In the present review, we summarize the functional role of C-type lectins in mosquitoes and the mechanism underlying the alteration of Cry toxin activity by CTLs. Furthermore, we outline future research directions on elucidating the resistance mechanism. This study provides a basis for understanding resistance, which can be used to develop novel insecticides.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2022.898198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136036PMC
May 2022

The First Report on Transgenic Hairy Root Induction from the Stem of Tung Tree ().

Plants (Basel) 2022 May 16;11(10). Epub 2022 May 16.

CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430074, China.

Tung tree () is an industrially important oil-bearing woody plant of the Euphorbiaceae family. Functional studies of tung tree at the molecular level are limited by the lack of an efficient transgenic system. The -mediated hairy root generation system is an alternative to typical plant transformation systems. However, its application in many plants has been blocked due to the inability of existing methods to induce hairy roots. Thus, it is critical to build a method suitable for the hairy induction of the specific plant of interest. In this study, a modified method for tung tree was developed, and it is the first report that hairy roots could be effectively induced in the stem of tung tree. With the method, an average of 10.7 hairy roots per seedling were generated in tung tree, approximately 67% of seedlings produced transgenic hairy roots and approximately 13.96% of the hairy roots of these seedlings were transgenic. This modified method is also suitable for the hairy root induction of two other oil-bearing plants of the Euphorbiaceae family, and . This modified method will accelerate functional studies of tung tree at the molecular level and also shed light on plants lacking a transgenic system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/plants11101315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148109PMC
May 2022

The Metabolism of a Novel Cytochrome P450 () in Tribenuron-Methyl-Resistant L. to Herbicides with Different Mode of Actions.

Int J Mol Sci 2022 May 22;23(10). Epub 2022 May 22.

Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.

L. (flixweeds) is a noxious broad-leaf weed infesting winter wheat fields in China that has evolved high resistance to tribenuron-methyl. In this work, a brand new gene was cloned from tribenuron-methyl-resistant (TR) and transferred into , and the sensitivities of with or without the transgene to herbicides with a different mode of actions (MoAs) were tested. Compared to expressing pCAMBIA1302-GFP (empty plasmid), transferring pCAMBIA1302-CYP77B34 (recombinant plasmid) became resistant to acetolactate synthase (ALS)-inhibiting herbicide tribenuron-methyl, protoporphyrinogen oxidase (PPO)-inhibiting herbicides carfentrazone-ethyl and oxyfluorfen. Cytochrome P450 inhibitor malathion could reverse the resistance to tribenuron-methyl, carfentrazone-ethyl and oxyfluorfen in transgenic plants. In addition, the metabolic rates of tribenuron-methyl in expressing were significantly higher than those in expressing pCAMBIA1302-GFP. Other than that, the transgenic plants showed some tolerance to very-long-chain fatty acid synthesis (VLCFAs)-inhibiting herbicide pretilachlor and photosystem (PS) II-inhibiting herbicide bromoxynil. Subcellular localization revealed that the CYP77B34 protein was located in the endoplasmic reticulum (ER). These results clearly indicated that mediated resistance to tribenuron-methyl and may have been involved in cross-resistance to carfentrazone-ethyl, oxyfluorfen, pretilachlor and bromoxynil.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms23105812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147942PMC
May 2022

Predicting Areas with High Concentration of the Long-Term Uninsured and Their Association with Emergency Department Usage by Uninsured Patients in South Carolina.

Healthcare (Basel) 2022 Apr 21;10(5). Epub 2022 Apr 21.

Department of Public Health Sciences, Clemson University, Clemson, SC 29634, USA.

Background: To predict areas with a high concentration of long-term uninsured (LTU) and Emergency Department (ED) usage by uninsured patients in South Carolina.

Methods: American Community Survey data was used to predict the concentration of LTU at the ZIP Code Tabulation Area (ZCTA) level. In a multivariate regression model, the LTU concentration was then modeled to predict ED visits by uninsured patients. ED data came from the restricted South Carolina Patient Encounter data with patients' billing zip codes. A simulation was conducted to predict changes in the ED visit numbers and rates by uninsured patients if the LTU concentration was reduced to a lower level.

Results: Overall, there was a positive relationship between ED visit rates by the uninsured patients and areas with higher concentrations of LTU. Our simulation model predicted that if the LTU concentration for each ZCTA was reduced to the lowest quintile, the ED visit rates by the uninsured would decrease significantly. The greatest reduction in the number of ED visits by the uninsured over a two-year period was for the following primary diagnoses: abdominal pain (15,751 visits), cellulitis and abscess (11,260 visits) and diseases for the teeth and supporting structures (10,525 visits).

Conclusions: The provision of primary healthcare services to the LTU could help cut back inappropriate uses of ED resources and healthcare costs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/healthcare10050771DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142117PMC
April 2022

Resveratrol ameliorates muscle atrophy in chronic kidney disease via the axis of SIRT1/FoxO1.

Phytother Res 2022 Aug 23;36(8):3265-3275. Epub 2022 May 23.

Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, China.

Chronic kidney disease (CKD) is often associated with muscle atrophy. However, the underlying molecular mechanisms are still not well understood. Here, we treated 5/6-nephrectomized (5/6Nx) rats with resveratrol and found that this treatment greatly improves renal function as evidenced by reduced proteinuria and cystatin C. Moreover, resveratrol ameliorates renal fibrosis by reducing transforming growth factor β (TGF-β) and connective tissue growth factor (CTGF). Meanwhile, muscle atrophy in these 5/6Nx rats was largely attenuated by resveratrol. Immunoprecipitation revealed that SIRT1 physically interacts with FoxO1 in muscle, and this interaction was weakened in 5/6Nx rats. As a consequence, acetylated FoxO1 was increased in muscle of 5/6Nx rats. The application of resveratrol markedly reverses this trend. These data point out that SIRT1 is a key factor for linking renal disease and muscle atrophy. Indeed, both renal dysfunction and muscle atrophy were further aggravated by 5/6Nx in Sirt1 mice. Taken together, our data indicate that SIRT1 plays a pivotal role in muscle atrophy in CKD, and FoxO1 might be a substrate of SIRT1 in this process. Furthermore, resveratrol, together with other agonists of SIRT1, may hold great therapeutic potentials for treating CKD and its related muscle atrophy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ptr.7499DOI Listing
August 2022

An Integrated Immune-Related Bioinformatics Analysis in Glioma: Prognostic Signature's Identification and Multi-Omics Mechanisms' Exploration.

Front Genet 2022 3;13:889629. Epub 2022 May 3.

Department of Emergency Medicine, Shangrao Hospital Affiliated to Nanchang University, Shangrao People's Hospital, Shangrao, China.

As the traditional treatment for glioma, the most common central nervous system malignancy with poor prognosis, the efficacy of high-intensity surgery combined with radiotherapy and chemotherapy is not satisfactory. The development of individualized scientific treatment strategy urgently requires the guidance of signature with clinical predictive value. In this study, five prognosis-related differentially expressed immune-related genes (PR-DE-IRGs) (CCNA2, HMGB2, CASP3, APOBEC3C, and BMP2) highly associated with glioma were identified for a prognostic model through weighted gene co-expression network analysis, univariate Cox and lasso regression. Kaplan-Meier survival curves, receiver operating characteristic curves and other methods have shown that the model has good performance in predicting the glioma patients' prognosis. Further combined nomogram provided better predictive performance. The signature's guiding value in clinical treatment has also been verified by multiple analysis results. We also constructed a comprehensive competing endogenous RNA (ceRNA) regulatory network based on the protective factor BMP2 to further explore its potential role in glioma progression. Numerous immune-related biological functions and pathways were enriched in a high-risk population. Further multi-omics integrative analysis revealed a strong correlation between tumor immunosuppressive environment/IDH1 mutation and signature, suggesting that their cooperation plays an important role in glioma progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2022.889629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114310PMC
May 2022

Green Space, Air Pollution, Weather, and Cognitive Function in Middle and Old Age in China.

Front Public Health 2022 2;10:871104. Epub 2022 May 2.

Department of Biostatistics, Shandong University, Jinan, China.

Prior research has shown that environmental hazards, such as limited green space, air pollution, and harmful weather, have the strong adverse impact on older adults' cognitive function; however, most of the studies were conducted in developed countries and limited to cross-sectional analyses. China has the largest aging population in the world so the research evidence from it can offer an insight to the study in other developing countries facing similar issues and inform future public health policy and disease control. This study examined the long-term impact of environmental factors, namely, green space coverage, air pollution, and weather conditions on cognitive function using a nationally representative sample consisting of adults aged 45 years and older selected from the China Health and Retirement Longitudinal Study (CHARLS 2011-2018), the China City Statistical Yearbook, and other sources. Multilevel growth curve models were utilized for analysis and the mediator effects of physical activity and social engagement on the relationship between environmental factors and cognitive function were examined. Findings of this study showed that after controlling for sociodemographic characteristics, annual precipitation of 80 cm or more, living in areas with July temperature of 28°C or higher, urban community, and green space coverage were positively associated with cognition score at the baseline and lower precipitation, urban community, and greater green space coverage were associated with slower cognitive decline over a 7-year period. The impact of gross domestic product (GDP) seemed to take into effect more and more over time. These effects did not substantially change after weekly total hours of physical activities and levels of social engagement were added. More research on the mechanisms of the effect of environmental factors on cognition is needed such as the subgroup analyses and/or with more aspects of environmental measures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpubh.2022.871104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108722PMC
May 2022

Contrasting responses in growth, photosynthesis and hydraulics of two subtropical tree species to cadmium contamination as affected by elevated CO and nitrogen addition.

Sci Total Environ 2022 Sep 10;837:155858. Epub 2022 May 10.

Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; College of Life Science, Gannan Normal University, Ganzhou, Jiangxi 341000, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Plant growth, photosynthesis, and hydraulics are affected by heavy metals but also by elevated atmospheric CO concentration (e[CO]) and nitrogen (N) deposition. However, few studies have investigated the response of woody species to the combined effects of these three factors. We conducted an open-top chamber experiment with two common subtropical trees (Acacia auriculiformis and Syzygium hainanense) to explore the effects of cadmium (Cd)-contamination, e[CO], and N addition on plant eco-physiological traits. We found that the growth of A. auriculiformis was insensitive to the treatments, indicating that it is a Cd-tolerant and useful afforestation species. For S. hainanense, in contrast, e[CO] and/or N addition offset the detrimental effects of Cd addition by greatly increasing plant biomass and reducing the leaf Cd concentration. We then found that e[CO] and/or N addition offset the detrimental Cd effects on S. hainanense biomass by increasing its photosynthetic rate, its N concentration, and the efficiency of its stem water transport network. These offsetting effects of e[CO] and/or N addition, however, came at the expense of reduced xylem hydraulic safety resulting from wider vessels, thinner vessel walls, and therefore weaker vessel reinforcement. Our study suggests that, given future increases in global CO concentration and N deposition, the growth of Cd-tolerant tree species (like A. auriculiformis) will be probably stable while the growth of Cd-sensitive tree species (like S. hainanense) might be enhanced despite reduced hydraulic safety. This also suggests that both species will be useful for afforestation of Cd-contaminated soils given future global change scenarios.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2022.155858DOI Listing
September 2022

Ziyuglycoside I attenuates collagen-induced arthritis through inhibiting plasma cell expansion.

J Ethnopharmacol 2022 Aug 6;294:115348. Epub 2022 May 6.

Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine, Hefei, Anhui Province, 230032, China. Electronic address:

Ethnobotanical Relevance: With most of the anti-rheumatic drugs having severe adverse drug reactions and poor tolerance, the active components from natural herbs provides a repository for novel, safe, and effective drug development. Sanguisorba officinalis L. exhibits definite anti-inflammatory capacity, however, whether it has anti-rheumatic effects has not been revealed.

Aim Of The Study: In the present study, the effect of Ziyuglycoside I (Ziyu I), one of the most important active components in Sanguisorba officinalis L., was investigated in treating collagen-induced arthritis (CIA), illuminating its potential pharmacological mechanisms.

Material And Methods: CIA mice were treated with 5, 10, or 20 mg/kg of Ziyu I or 2 mg/kg of MTX, and clinical manifestations as well as pathological changes were observed. T and B cell viability was determined using cell counting kit-8, plasma autoantibodies and cytokines were tested with ELISA, T and B cell subsets were identified by flow cytometry, Blimp1 expression was detected by RT-qPCR and in situ immunofluorescence. The expression of activation-induced cytidine deaminase (AID) was detected by immunohistochemistry. ERK activation in B cells was verified through western blotting and immunofluorescence. Meanwhile, bioinformatics retrieval and molecular docking/molecular dynamics were used to predict the relationship between Blimp1, ERK and Ziyu I with the pharmacokinetics and toxicity of Ziyu I being evaluated in the ADMETlab Web platform.

Results: Ziyu I treatment effectively alleviated the joint inflammatory manifestation including arthritis index, global scores, swollen joint count and body weight of CIA mice. It improved the pathological changes of joint and spleen of arthritic mice, especially in germinal center formation. Ziyu I displayed a moderate regulatory effect on T cell activation, the percentage of total T and helper T cells, and tumor necrosis factor-α, but transforming growth factor-β was not restored. Increased spleen index, B cell viability and plasma auto-antibody production in CIA mice were significantly reduced by Ziyu I therapy. Of note, we found that Ziyu I administration substantially inhibited the excessive expansion of plasma cells in spleen through preventing the expression of B lymphocyte induced maturation protein 1 (Blimp1) and AID in B cells. Ziyu I was predicted in silico to directly interact with ERK2, and reduce ERK2 activation, contributing to the depressed expression of Blimp1. Moreover, Ziyu I was predicted to have a favorable pharmacokinetic profile and low toxicity.

Conclusion: Ziyu I effectively ameliorates CIA in mice by inhibiting plasma cell generation through prevention of ERK2-mediated Blimp1 expression in B cells. Therefore, Ziyu I is a promising candidate for anti-arthritic drug development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jep.2022.115348DOI Listing
August 2022

Recycling of memory B cells between germinal center and lymph node subcapsular sinus supports affinity maturation to antigenic drift.

Nat Commun 2022 05 5;13(1):2460. Epub 2022 May 5.

Institute of Immunology and Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.

Infection or vaccination leads to the development of germinal centers (GC) where B cells evolve high affinity antigen receptors, eventually producing antibody-forming plasma cells or memory B cells. Here we follow the migratory pathways of B cells emerging from germinal centers (B) and find that many B cells migrate into the lymph node subcapsular sinus (SCS) guided by sphingosine-1-phosphate (S1P). From the SCS, B cells may exit the lymph node to enter distant tissues, while some B cells interact with and take up antigen from SCS macrophages, followed by CCL21-guided return towards the GC. Disruption of local CCL21 gradients inhibits the recycling of B cells and results in less efficient adaption to antigenic variation. Our findings thus suggest that the recycling of antigen variant-specific B cells and transport of antigen back to GC may support affinity maturation to antigenic drift.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-022-29978-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072412PMC
May 2022

G protein-coupled receptor kinase type 2 and β-arrestin2: Key players in immune cell functions and inflammation.

Cell Signal 2022 07 21;95:110337. Epub 2022 Apr 21.

Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Collaborative Innovation Center of Anti-inflammatory and Immune Medicine, Hefei 230032, China. Electronic address:

G protein-coupled receptor kinase type 2 (GRK2) and β-arrestin2 are representative proteins that regulate the transduction and trafficking of G protein-coupled receptor (GPCR) signaling. The kinase GRK2 and the multifunctional scaffolding protein β-arrestin2 are key integrated signaling nodes in various biological processes, and both of them regulate cell proliferation and promote cell invasion and migration. GRK2/β-arrestin2 play multiple roles in the pathological mechanisms of a wide range of diseases including heart failure, cancer, and inflammatory diseases. This review summarizes the roles of GRK2/β-arrestin2 in immune cell function and focuses on the pathological implications of GRK2/β-arrestin2 in various inflammatory diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cellsig.2022.110337DOI Listing
July 2022

Transport and deposition of solid phosphorus-based mineral particles in urine diversion systems.

Environ Technol 2022 Apr 25:1-13. Epub 2022 Apr 25.

Centre for Environmental Health Engineering (CEHE), Department of Civil and Environmental Engineering, Faculty of Engineering and Physical Sciences, University of Surrey, Surrey, UK.

The deposition of solid phosphorus-based mineral particles is a common problem in urine diversion systems, which occurs in transport systems, particularly in horizontal pipelines. In this work, particle deposition behaviour in turbulent flow in a 3D horizontal pipe was simulated by using the Euler-Lagrange method. The effects of particle diameter, particle density, particle shape factor and fluid flow velocity on particle deposition behaviour were investigated. The results showed that the deposition rate increased by 9.92%,6.88% and 6.88% with increasing particle diameter (10-90 ), particle density (1400 kg/m-2300 kg/m), and particle shape factor (0.2-1), respectively. For particles with larger diameters (>90 ) or larger density (>2300 kg/m), the deposition rate of these particles was almost reached 100%. It was found that gravitational sedimentation was the dominant deposition mechanism in low fluid flow velocity range (0.1-0.5 m/s). As fluid flow velocity increased (>0.5 m/s), turbulent fluctuation became the dominant factor that affected particle motion behaviour, whereas the effect of gravitational sedimentation on particle deposition behaviour declined significantly, and the increase in fluid flow velocity no longer significantly affects deposition rate. It was found that the deposition rate decreased by 29.13% as the fluid flow velocity was increased from 0.1 m/s to 0.5 m/s, while the corresponding deposition rate only decreased by 14.24% when the fluid flow velocity was increased from 0.5 m/s to 2 m/s. The optimal flow velocity was found to range between 0.75 and 1.25 m/s, which may mitigate the deposition of mineral solids in urine diversion systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/09593330.2022.2068378DOI Listing
April 2022

The Application of the Omaha System in Community Rehabilitation Nursing for Patients With Stroke and Previous Falls.

Front Neurol 2022 31;13:711209. Epub 2022 Mar 31.

School of Nursing, Nanchang University, Nanchang, China.

Objective: This study aimed to explore the use of the Omaha System in rehabilitation and nursing methods and the effects on patients within the community who had experienced stroke and previous falls.

Methods: This study enrolled 42 patients who had experienced stroke and previous falls and had returned to the community after being discharged from the Department of Neurology and Rehabilitation of the Affiliated Hospital of Nanchang University from January to July 2018. The patients were randomly divided into two groups: an experimental group ( = 21) and a control group ( = 21). Patients in the control group received routine community rehabilitation care, and patients in the experimental group received community rehabilitation care on the basis of the Omaha System. Intervention lasted for 1 year. The Omaha outcome score, the ability to perform activities of daily living (ADL) (measured via the Modified Barthel Index [MBI]), and the incidence of falls for each group were compared before and after the intervention.

Results: After 1 year of intervention, the Omaha outcome score and MBI of both groups were higher than before; the Omaha outcome score and MBI of the experimental group were higher than those of the control group; the differences were statistically significant ( < 0.05). No fall occurred in either of the two groups.

Conclusion: The Omaha System can comprehensively evaluate the health problems of patients, guide nursing intervention, and quantitatively evaluate the effect of nursing intervention; it is therefore worthy of promotion.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2022.711209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008722PMC
March 2022

Mutant p53 achieved Gain-of-Function by promoting tumor growth and immune escape through PHLPP2/AKT/PD-L1 pathway.

Int J Biol Sci 2022 14;18(6):2419-2438. Epub 2022 Mar 14.

School of Biomedical Sciences, Hunan University, Changsha, China.

The most frequent genetic alterations of the TP53 gene in human cancer were reported. TP53 mutation gains new function as a target of genetic instability, which is associated with increased tumor progression and poor survival rate in patients. In this study, more than three hundred colorectal cancer patients' samples were firstly analyzed, and the results showed that patients with mutant p53 had higher levels of AKT phosphorylation and PD-L1 expression, which were next verified both in cell lines and patients' samples . Further studies demonstrated that the hotspot of mutant p53 directly binds to the promoter of PHLPP2 to inhibit its transcription, and resulting in down-regulating its protein expressional level. Subsequently, AKT was released and activated, promoting tumor proliferation and metastasis. In parallel, 4EBP1/eIF4E was identified as downstream executors of AKT to enhance the translational level of PD-L1, which decreased the activation of T cells. Besides, inhibiting AKT/mTOR pathway significantly suppressed PD-L1 expression, tumor growth, and immune escape in p53 mutated cells. In conclusion, mutant p53 achieved its Gain-of-Function by transcriptionally inhibiting PHLPP2 and activating AKT, which suppresses immune response and advances tumor growth. Thus, this study provides an excellent basis for a further understanding of the clinical treatment of neoplastic diseases for patients with mutant p53, with an emphasis on immunotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/ijbs.67200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8990467PMC
March 2022
-->