Publications by authors named "Lingling Wang"

820 Publications

Knowledge domain and emerging trends of climate-smart agriculture: a bibliometric study.

Environ Sci Pollut Res Int 2022 May 19. Epub 2022 May 19.

School of Management and Economics, Beijing Institute of Technology, Beijing, 100081, China.

Climate-smart agriculture (CSA) is a new agricultural development pattern to address future food crises. Since CSA was proposed in 2010, it has attracted the attention of scholars from all over the world. It is of great significance to scientifically summarize the overview and emerging trends of CSA research, providing ideas for scholars concerned about CSA to engage in research in this field. Based on bibliometrics and CSA-related literature data in the WOS database, this paper used CiteSpace software to draw knowledge maps to scientifically analyze publications in the field of CSA. Our study found that (1) CSA research is showing a rapid upward trend, focusing on the environmental sciences and agricultural economic management; (2) international organizations such as the FAO, World Bank, and the international agricultural research institute have made significant contributions to CSA research; (3) among the nine clusters in the CSA literature, CSA practice, conservation agriculture, smallholder farmers, and sub-Saharan Africa have been consistently given high attention; (4) CSA research can be divided into three phases, and the research hotspots have transferred from essential elements of CSA to household and carbon emissions. We believe that in future research, more attention should be paid to the trade-off and synergy of the three pillars of CSA, as well as the investment, finance, and evaluation criteria of CSA. Such strengthening is of great significance to the sustainable promotion of CSA.
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http://dx.doi.org/10.1007/s11356-022-20796-9DOI Listing
May 2022

Thioredoxin 1 regulates the pentose phosphate pathway via ATM phosphorylation after experimental subarachnoid hemorrhage in rats.

Brain Res Bull 2022 May 16;185:162-173. Epub 2022 May 16.

Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China; Institute of Stroke Research, Soochow University, Suzhou 215006, China. Electronic address:

Subarachnoid hemorrhage (SAH), a type of hemorrhagic stroke, is a neurological emergency with high morbidity and mortality. Early brain injury (EBI) after SAH is the leading cause of poor prognosis in SAH patients. TRX system is a NADPH-dependent antioxidant system which is composed of thioredoxin reductase (TRXR), thioredoxin (TRX). The pentose phosphate pathway (PPP), a pathway through which glucose can be metabolized, is a major source of NADPH. Thioredoxin 1 (TRX1) is a member of thioredoxin system mainly located in cytoplasm. Serine/threonine kinases ataxia telangiectasia mutated (ATM) is an important oxidative stress receptor, and TRX1 can regulate ATM phosphorylation and then affect the activity of PPP key enzyme glucose 6-phosphate dehydrogenase (G6PD). However, whether TRX1 is involved in the regulation of PPP pathway after subarachnoid hemorrhage remains unclear. The results showed that after SAH, the level of TRX1 and phosphor-ATM decreased while the level of TRXR1 increased. G6PD protein level remained unchanged but the activity decreased, and the NADPH contents decreased. Overexpression of TRX1 by lentivirus upregulates the level of phosphor-ATM, G6PD activity and NADPH content. TRX1 overexpression improved short-term and long-term neurobehavioral outcomes and alleviated neuronal impairment in rats. Nissl staining showed that upregulation of TRX1 reduced cortical neuron injury. Our study shows that TRX1 participates in the PPP pathway by regulating phosphorylation ATM, which is accomplished by affecting G6PD activity. TRX1 may be an important target for EBI intervention after SAH.
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http://dx.doi.org/10.1016/j.brainresbull.2022.05.008DOI Listing
May 2022

A DFT study on the structure activity relationship of the natural xanthotoxin-based pharmaceutical cocrystals.

J Mol Model 2022 May 17;28(6):155. Epub 2022 May 17.

College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040, China.

In this work, the pharmaceutical cocrystals xanthotoxin-para-aminobenzoic acid (XT-PABA) and xanthotoxin-oxalic acid (XT-OA) were systematically investigated in the gas and water phases by using the quantum chemical approach. The weak intermolecular interactions have been estimated and the O1…H4 (O1…H5) intermolecular hydrogen bond (IHB) with moderate intensity and partial covalent natures was confirmed based on the computed structural parameters, topology analysis, and reduced density gradient (RDG) isosurfaces. The electrophilic and nucleophilic reactivities of different positions associated with intermolecular interactions in XT, PABA, and OA were predicted by plotting the molecular electrostatic potential (MESP) diagrams. The calculated natural bond orbital (NBO) population analysis has quantitatively unveiled the intrinsic reason for the variations in weak intermolecular interactions within XT-PABA and XT-OA cocrystals, from the gas phase to the water phase. Besides, the frontier molecular orbitals (FMOs), Fukui function, and various global reactivity descriptors were computed to measure the chemical reactivity of all the investigated molecular systems. The XT-PABA and XT-OA cocrystals explored in this work could be regarded as valuable exemplar systems to design and synthesize the high-efficiency pharmaceutical cocrystals in the experiment.
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http://dx.doi.org/10.1007/s00894-022-05152-5DOI Listing
May 2022

The truncated MyD88s negatively regulates TLR2 signal on expression of IL17-1 in oyster Crassostrea gigas.

Dev Comp Immunol 2022 May 13;133:104446. Epub 2022 May 13.

Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Functional Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Diseases Prevention and Control, Dalian Ocean University, Dalian, 116023, China. Electronic address:

Toll like receptor (TLR) signaling plays a key role in the innate immune recognition and inflammatory regulation in both vertebrates and invertebrates. The expanded TLR signaling components, including 83 TLRs and 10 MyD88s, have been reported in the genome of the Pacific oyster Crassostrea gigas. In the present study, one endogenous TLR (designated CgTLR2) and two MyD88s (including a full-length CgMyD88-2 containing intact TIR domain and Death-domain, and a truncated CgMyD88s with only TIR domain) were identified from oyster C. gigas. CgTLR2 was highly expressed in haemocytes, especially in granulocytes. The recombinant protein of the extracellular LRR domains of CgTLR2 recognized and bound a variety of PAMPs with the strongest binding capability to LPS. The recombinant protein of intracellular TIR domain of CgTLR2 was able to bind the recombinant proteins of rCgMyD88-2 (K = 1.96 × 10 M) and rCgMyD88s (K = 4.84 × 10 M), with higher affinity towards rCgMyD88-2. After Vibrio splendidus stimulation, the mRNA expression levels of CgTLR2 and CgMyD88-2 were rapidly up-regulated at early stage of immune response (from the 3rd hours after V. splendidus stimulation), while that of CgMyD88s did not change until 24 h post stimulation. When CgTLR2 was knocked-down by siRNA interference, the expression levels of CgMyD88-2 and CgMyD88s decreased significantly, concomitant with the down-regulation of expression of CgIL17-1. After the expression of CgMyD88-2 was interfered, the expressions of CgMyD88s and CgIL17-1 were all decreased. In contrast, after the expression of CgMyD88s was interfered, the expressions of CgMyD88-2 and CgIL17-1 all increased. The results showed that CgMyD88s played a negative role in the regulation of CgTLR2 on inflammatory factor CgIL17-1.
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http://dx.doi.org/10.1016/j.dci.2022.104446DOI Listing
May 2022

Cortisol modulates glucose metabolism and oxidative response after acute high temperature stress in Pacific oyster Crassostrea gigas.

Fish Shellfish Immunol 2022 May 10. Epub 2022 May 10.

Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.

Cortisol is the main stress hormone that plays crucial roles in energy metabolism and immune response in vertebrates. In the present study, the homologues of 11β-hydroxysteroid dehydrogenase type 1 (designated Cg11β-HSD1) and 5α-reductase 1 (designated Cg5αR1), the key enzymes related to cortisol metabolism, were identified from Pacific oyster Crassostrea gigas. The Cg11β-HSD1 harbored a conserved SDR domain, and Cg5αR1 contained a Steroid_dh domain and three transmembrane domains. The mRNA transcripts of Cg11β-HSD1 and Cg5αR1 were constitutively expressed in all the examined tissues of oysters, with the highest expression level in haemocytes and labial palp, respectively. After acute high temperature stress (28 °C), the mRNA expression level of Cg11β-HSD1 in hepatopancreas significantly up-regulated at 6 h and 12 h, and that of Cg5αR1 significantly up-regulated at 6 h, compared with the Blank group (11 °C). The concentration of cortisol and glucose, as well as the activities of superoxide dismutase (SOD) and catalase (CAT) in hepatopancreas all significantly up-regulated after acute high temperature stress, while the glycogen concentration in adductor muscle decreased significantly at 6 h and 12 h. After the blockage of Cg11β-HSD1 with metyrapone (MET), the cortisol concentration and the activities of SOD and CAT significantly decreased after acute high temperature stress, the glucose concentration in hepatopancreas significantly increased at 24 h, and the glycogen concentration in adductor muscle significantly increased at 6 h. These results collectively suggested that cortisol played a crucial role in regulating glucose metabolism and oxidative response in oysters after acute high temperature stress.
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http://dx.doi.org/10.1016/j.fsi.2022.05.010DOI Listing
May 2022

Axl Mediates Resistance to Respiratory Syncytial Virus Infection Independent of Cell Attachment.

Am J Respir Cell Mol Biol 2022 May 12. Epub 2022 May 12.

State Key Laboratory of Pharmaceutical Biotechnology, 384750, Medical School, Nanjing University, Nanjing, China.

Respiratory syncytial virus (RSV) is a leading cause of severe lower respiratory tract infections in infants and young children. Axl, a TAM family receptor tyrosine kinase (RTK), has been demonstrated as a receptor mediating enveloped virus infection. Here we show that Axl functions as a suppressor of antiviral response during RSV infection. Knockdown of Axl expression in human cells resulted in cell resistance to RSV infection although the treatment did not significantly affect RSV binding or cell entry. Mice deficiency of Axl showed resistance to RSV infection including reduction in viral load and in pulmonary injury. Although T lymphocyte and macrophage infiltration was reduced, more IFN-γ producing cells were present in BALF in Axl-/- mice. Less alternatively activated alveolar macrophages were found in the lungs of Axl-/- mice. Axl-/- MEF cells and siRNA-treated human cells had more robust IFN-β and ISG induction of antiviral genes. Furthermore, re-expression of Axl using Ad-mediated Axl delivery repressed ISG induction in Axl-null MEF cells by RSV infection. The results suggest that Axl, independent of being a virus entry receptor of RSV infection, negatively regulates interferon signaling to modulate host antiviral response against RSV infection.
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http://dx.doi.org/10.1165/rcmb.2021-0362OCDOI Listing
May 2022

Induction of neutralizing antibodies against SARS-CoV-2 variants by a multivalent mRNA-lipid nanoparticle vaccine encoding SARS-CoV-2/SARS-CoV Spike protein receptor-binding domains.

bioRxiv 2022 Apr 28. Epub 2022 Apr 28.

To address the need for multivalent vaccines against that can be rapidly developed and manufactured, we compared antibody responses against SARS-CoV, SARS-CoV-2, and several variants of concern in mice immunized with mRNA-lipid nanoparticle vaccines encoding homodimers or heterodimers of SARS-CoV/SARS-CoV-2 receptor-binding domains. All vaccine constructs induced robust anti-viral antibody responses, and the heterodimeric vaccine elicited an IgG response capable of cross-neutralizing SARS-CoV, SARS-CoV-2 Wuhan-Hu-1, B.1.351 (beta), and B.1.617.2 (delta) variants.
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http://dx.doi.org/10.1101/2022.04.28.489834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094100PMC
April 2022

Homozygous Loss of , but not its Haploinsufficiency, Leads to Male Infertility and Fertilization Failure.

Front Cell Dev Biol 2022 25;10:850052. Epub 2022 Apr 25.

Tianjin Union Medical Center, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Sciences, Frontiers Science Center for Cell Responses, National Demonstration Center for Experimental Biology Education and College of Life Sciences, Nankai University, Institute of Translational Medicine, Tianjin, China.

The gene has been associated with male infertility. Male chimera mice were infertile, supporting the prevailing view that haploinsufficiency causes male infertility. In this study, we identified a heterozygous mutation on , c.72C>A (p.Cys24Ter) in the male partner of a patient couple, who had a previous fertilization failure (FF) after intracytoplasmic sperm injection (ICSI) and became pregnant after ICSI together with artificial oocyte activation (AOA). To investigate the role of in FF and oocyte activation, we constructed knockout mice. Surprisingly, male mice, but not male mice, are infertile, and have reduced sperm counts and abnormal sperm morphology. Importantly, AOA treatment enhances the 2-cell embryo rate of ICSI embryos injected with sperm, indicating that FF caused by male deficiency is overcome by AOA. Mechanistically, loss of PLCζ around the acrosome might be the reason for FF of sperm. Taken together, our data indicated that homozygous knockout of , but not haploinsufficiency, leads to male infertility and FF.
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http://dx.doi.org/10.3389/fcell.2022.850052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082362PMC
April 2022

CgHMGB1 functions as a broad-spectrum recognition molecule to induce the expressions of CgIL17-5 and Cgdefh2 via MAPK or NF-κB signaling pathway in Crassostrea gigas.

Int J Biol Macromol 2022 May 4;211:289-300. Epub 2022 May 4.

Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Southern Laboratory of Ocean Science and Engineering (Guangdong, Zhuhai), Zhuhai 519082, China; Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian 116023, China.

High-mobility group box 1 (HMGB1), a highly conserved nucleoprotein, functions in immune recognition, inflammation and antibacterial immunization in vertebrates. In the present study, the mediation mechanism of CgHMGB1 in activating MAPK and NF-κB/Rel signaling pathways to induce the expressions of immune effectors was investigated. CgHMGB1 mRNA was detected in all tested developmental stages from fertilized egg to D-larvae, with the higher expressions in 4 cells and 8 cells stages. CgHMGB1 proteins were mainly distributed in haemocyte granulocytes. The expressions of CgHMGB1 mRNA in haemocytes increased significantly after Vibrio splendidus stimulation, and CgHMGB1 protein translocated into the haemocyte cytoplasm and release into cell-free haemolymph. The phosphorylation of CgERK and CgP38 were induced, the nuclear translocation of CgRel were promoted, and the mRNA expressions of CgIL17-5 and Cgdefh2 increased significantly after rCgHMGB1 treatment. Obvious branchial swelling and cilium shedding were observed after rCgHMGB1 treatment. rCgHMGB1 exhibited binding activity to different polysaccharides, bacteria, and fungi. rCgHMGB1 also displayed obvious antibacterial activity to V. splendidus and E. coli. These results indicated that CgHMGB1 functioned as an immune recognition molecule to recognize various PAMPs and bacteria to induce the mRNA expressions of CgIL17-5 and Cgdefh2 via the activation of MAPK and NF-κB signaling pathways in oysters.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.04.166DOI Listing
May 2022

Genetic Characterization of Four Groups of Chromosome-Borne Accessory Genetic Elements Carrying Drug Resistance Genes in .

Infect Drug Resist 2022 27;15:2253-2270. Epub 2022 Apr 27.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, People's Republic of China.

Purpose: The aim of this study was to gain a deeper genomics and bioinformatics understanding of diversification of accessory genetic elements (AGEs) in .

Methods: Herein, the complete genome sequences of five isolates from China were determined, and seven AGEs were identified from the chromosomes. Detailed genetic dissection and sequence comparison were applied to these seven AGEs, together with additional 10 chromosomal ones from GenBank (nine of them came from ).

Results: These 17 AGEs were divided into four groups: Tn and its six derivatives, Tn and its two derivatives, Tn and its one derivative, and Tn and its four derivatives. These AGEs display high-level diversification in modular structures that had complex mosaic natures, and particularly different multidrug resistance (MDR) regions were presented in these AGEs. At least 52 drug resistance genes, involved in resistance to 15 different categories of antimicrobials and heavy metal, were found in 15 of these 17 AGEs.

Conclusion: Integration of these AGEs into the chromosomes would contribute to the accumulation and distribution of drug resistance genes and enhance the ability of isolates to survive under drug selection pressure.
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http://dx.doi.org/10.2147/IDR.S354934DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058013PMC
April 2022

Association analysis for SNPs of and genes with body size and other production traits in Liaoning Cashmere Goats.

Anim Biotechnol 2022 May 4:1-11. Epub 2022 May 4.

College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.

Liaoning cashmere goat (LCG) have tall bones, high cashmere production and outstanding meat production performance. In recent years, good breeding progress has not been made in terms of body size, meat yield, milk yield and other properties in terms of production. The study focused on the correlation between the SNPs of and genes with the body size performance, cashmere production and milk performance. The and gene sequence alignment and PCR-Seq polymorphism were used to detect the potential SNPs, and the correlation with production performance was analyzed by SPSS and SHEsis software. The results showed that the TT genotype at the T1662G locus of the gene is dominant and has significant advantages in body measurements such as sacrum height, chest width, and waist height. The C allele at the C4021T locus of gene shows an advantage in the body measurement performance. Among the haplotype combinations, H2H2:TGTC is preponderant combination for body size performance, H2H2:TGTC and H1H2:TGCC are preponderant combinations for cashmere production performance, H1H3:GGCC is preponderant combination for milk production performance. It may be a molecular marker for future selection and breeding.
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http://dx.doi.org/10.1080/10495398.2022.2051043DOI Listing
May 2022

Association analysis of single-nucleotide polymorphism in prolactin and its receptor with productive and body conformation traits in Liaoning cashmere goats.

Arch Anim Breed 2022 21;65(2):145-155. Epub 2022 Apr 21.

College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.

The results of this study showed that the single-nucleotide polymorphism (SNP) sites of the and genes have a certain association with the milk production performance, body size and cashmere performance of Liaoning cashmere goats (LCGs). Through our designed experiment, the potential SNPs of LCG were detected by sequence alignment, and two SNPs were found on two genes. The CC genotype of the gene is the dominant genotype among the three genotypes. The GG genotype of the gene is the dominant genotype among the two genotypes. At the same time, the two genotypes also have good performance in cashmere production and body size. Through the screening of haplotype combination, the milk fat rate  7.6 %, the milk protein rate  5.6 %, the milk somatic cell number  1500   10  mL , the cashmere fineness  15.75  m, the chest girth  105 cm, the chest depth  33 cm, and the waist height  67.5 cm are considered as screening indexes for comprehensive production performance of Liaoning cashmere goats. It is concluded that the GCGC type is the dominant haplotype combination. According to our research data, we found that the biological indicators of Liaoning cashmere goat milk are higher than the national standards, so we think it is very significant to study the milk production performance of our experiment. Further research can be done on goat milk production and body conformation traits around gene and gene.
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http://dx.doi.org/10.5194/aab-65-145-2022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051658PMC
April 2022

Network pharmacology combined with molecular docking to explore the potential mechanisms for the antioxidant activity of Rheum tanguticum seeds.

BMC Complement Med Ther 2022 May 3;22(1):121. Epub 2022 May 3.

Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, China.

Background: Rheum tanguticum (R. tanguticum) is an edible and medicinal plant that exhibits high antioxidant activity. The purpose of the present study was to investigate the bioactive components of its seeds and the potential mechanisms of antioxidant activity to provide a foundation for further developmental work on R. tanguticum seeds as a functional food.

Methods: In this study, the antioxidant activities of R. tanguticum seeds were measured using DPPH, ABTS and FRAP assays. LC-Q-TOF/MS was used to identify the active compounds in the seeds, and Swiss Target Prediction was used to identify their potential targets. The DisGENET, DrugBank, OMIM and GeneCard databases were used to search for antioxidant-related targets.

Results: The component-target-pathway network was constructed and included 5 compounds and 9 target genes. The hub genes included ESR1, APP, MAPK8, HSP90AA1, AKT1, MMP2, PTGS2, TGFB1 and JUN. The antioxidant activity signaling pathways of the compounds for the treatment of diseases were the cancer signaling pathway, estrogen signaling pathway, colorectal cancer signaling pathway, MAPK signaling pathway, etc. Molecular docking revealed that the compounds in R. tanguticum seeds could inhibit potential targets (AKT1, ESR1 and PTGS2).

Conclusion: Molecular docking studies revealed that the binding energy score between liriodenine and PTGS2 was the highest (8.16), followed by that of chrysophanol (7.10). This result supports the potential for PTGS2-targeted drug screening and design.
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http://dx.doi.org/10.1186/s12906-022-03611-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066831PMC
May 2022

Milk fat globule-epidermal growth factor-factor 8 decreased neuronal apoptosis and neuroinflammation to ameliorate early brain injury induced by subarachnoid hemorrhage through the inhibition of high mobility group box-1.

Hum Exp Toxicol 2022 Jan-Dec;41:9603271221093635

Department of Neurology, 519688Yantaishan Hospital, Yantai, China.

Aim: Both MFGE8 and HMGB1 were vital players for aneurysmal subarachnoid hemorrhage. However, whether HMGB1 was served as the downstream target of MFGE8 was unknown. To test this new mechanism, we performed the SAH model in rats.

Method: All treatments were injected intraventricularly into the right lateral ventricles. SAH grade, brain water content, and neurological function scores were evaluated. HMGB1 expression was studied by double immunofluorescence staining. HE and Nissl's staining were performed to observe the pathological change. Inflammatory factors were measured by ELISA method.

Results: High expression of MFGE8 could improve neurological function and reduce the brain edema and pro-inflammatory factors. Injection of rhMFGE8 inhibited HMGB1. To further verify the regulation of MFGE8 in HMGB1, we used rhHMGB1 and glycyrrhizin, and the results indicated MFGE8 produced excellent effect on SAH rats via inhibiting HMGB1.

Conclusion: In a word, MFGE8 improved EBI caused by SAH, depending on HMGB1 that was the potential mechanism.
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http://dx.doi.org/10.1177/09603271221093635DOI Listing
May 2022

Externalizing Behavior Problems Among Hui Ethnicity Left-Behind Children in Rural China: A Cross-Sectional Study.

Psychiatry Investig 2022 Apr 22;19(4):289-301. Epub 2022 Apr 22.

Mental Health Education Consulting Center, College of Clinic Medicine, Ningxia Medical University, Yinchuan, China.

Objective: This study aimed to investigate the prevalence of externalizing behavior problems (EBPs) and its influencing factors among Hui left-behind children (LBC) in rural China.

Methods: A cross-sectional study was conducted among school students from the southern rural areas in Ningxia, China (2012-2013). The general self-made questionnaire, Egma Minnen av Bardndosna Uppforstran, Eysenck Personality Questionnaire (for Children), Piers-Harris Children's Self-Concept Scale, and Achenbach's Child Behavior Checklist (for parents) were used to investigate the related information. Binary logistic regressions were conducted.

Results: The prevalence of EBPs in boys Hui LBC was significantly higher than that of non-LBC (12.37% vs. 6.84%, χ2=4.09, and p=0.04). Multivariate logistic regression analysis showed that low self-awareness of behavior (odds ratio [OR]=29.78), introversion (OR=21.67) and intermediate personality (OR=15.83), poor academic performance (OR=11.65) and both parent migrating (OR=2.73) were the risk factors for the EBPs of Hui LBC, while middle and high father refusal and denial (OR=0.11, OR=0.09) were their protective factors.

Conclusion: Our findings suggest that both parent migrating is a potential risk factor for EBPs among Hui LBC. Hui boys LBC should be paid more attention when formulating relevant policies.
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http://dx.doi.org/10.30773/pi.2021.0119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058266PMC
April 2022

First-Trimester Impetigo Herpetiformis Leads to Stillbirth: A Case Report.

Dermatol Ther (Heidelb) 2022 May 2;12(5):1271-1279. Epub 2022 May 2.

Department of Dermatology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, No. 261, Huansha Road, Hangzhou, China.

Introduction: Pustular psoriasis of pregnancy (PPP), also known as impetigo herpetiformis (IH), is a rare variant of generalized pustular psoriasis (GPP) in pregnancy. It typically occurs in the third trimester and is a life-threatening condition for both the pregnant mother and the fetus if not diagnosed and treated promptly. Drug-induced PPP has been reported in sporadic case reports. Here we present a case of first-trimester PPP occurring after applying drugs including chloroquine, which we consider a possible culprit triggering the disease.

Case Report: A 29-year-old female was admitted to our department at 45 days gestation with sudden onset of fever and widespread erythematous pustules for 9 days. She had been on medications including hydroxychloroquine before onset. The eruptions and systemic symptoms were controlled with high-dose systemic steroids; however, she was detected to have a stillbirth, and underwent dilation and curettage of the uterine. At the latest follow-up about 2 years after her admission, she reported to have delivered a healthy baby about 1 month previously.

Conclusions: Chloroquine has potential to lead to PPP in the first trimester of pregnancy. Further studies are warranted to investigate the etiology and treatment of PPP to facilitate early recognition and optimal management of this relatively rare dermatosis in pregnancy.
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http://dx.doi.org/10.1007/s13555-022-00735-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059450PMC
May 2022

Establishment of a Prognostic Model of Lung Adenocarcinoma Based on Tumor Heterogeneity.

Front Mol Biosci 2022 11;9:807497. Epub 2022 Apr 11.

Department of Respiratory and Critical Care Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Lung cancer is one of the main cancer types due to its persistently high incidence and mortality, yet a simple and effective prognostic model is still lacking. This study aimed to identify independent prognostic genes related to the heterogeneity of lung adenocarcinoma (LUAD), generate a prognostic risk score model, and construct a nomogram in combination with other pathological characteristics to predict patients' overall survival (OS). A significant amount of data pertaining to single-cell RNA sequencing (scRNA-seq), RNA sequencing (RNA-seq), and somatic mutation were used for data mining. After statistical analyses, a risk scoring model was established based on eight independent prognostic genes, and the OS of high-risk patients was significantly lower than that of low-risk patients. Interestingly, high-risk patients were more sensitive and effective to immune checkpoint blocking therapy. In addition, it was noteworthy that CCL20 not only affected prognosis and differentiation of LUAD but also led to poor histologic grade of tumor cells. Ultimately, combining risk score, clinicopathological information, and CCL20 mutation status, a nomogram with good predictive performance and high accuracy was established. In short, our research established a prognostic model that could be used to guide clinical practice based on the constantly updated big multi-omics data. Finally, this analysis revealed that CCL20 may become a potential therapeutic target for LUAD.
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http://dx.doi.org/10.3389/fmolb.2022.807497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035852PMC
April 2022

α-Branched amines through radical coupling with 2-azaallyl anions, redox active esters and alkenes.

Chem Sci 2022 Mar 3;13(13):3740-3747. Epub 2022 Mar 3.

Roy and Diana Vagelos Laboratories Penn/Merck Laboratory for High-Throughput Experimentation Department of Chemistry, University of Pennsylvania 231 South 34th Street Philadelphia PA USA

α-Branched amines are fundamental building blocks in a variety of natural products and pharmaceuticals. Herein is reported a unique cascade reaction that enables the preparation of α-branched amines bearing aryl or alkyl groups at the β- or γ-positions. The cascade is initiated by reduction of redox active esters to alkyl radicals. The resulting alkyl radicals are trapped by styrene derivatives, leading to benzylic radicals. The persistent 2-azaallyl radicals and benzylic radicals are proposed to undergo a radical-radical coupling leading to functionalized amine products. Evidence is provided that the role of the nickel catalyst is to promote formation of the alkyl radical from the redox active ester and not promote the C-C bond formation. The synthetic method introduced herein tolerates a variety of imines and redox active esters, allowing for efficient construction of amine building blocks.
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http://dx.doi.org/10.1039/d2sc00500jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8966660PMC
March 2022

Exposure assessment and risk-based limit levels evaluation of ochratoxin A in Astragali Radix in China.

Ecotoxicol Environ Saf 2022 Jun 13;237:113517. Epub 2022 Apr 13.

Yangtze Delta Region Institute of Tsinghua University, Zhejiang 314006, China. Electronic address:

Ochratoxin A (OTA) is a mycotoxin found in a variety of foods and herbal medicines, and several governmental bodies around the world have set maximum allowable levels of OTA in different foods and herbal medicines. This study aims to evaluate the health risk of OTA in Astragali Radix (AR) in China, and to evaluate the effects of different limit levels on the risk control of OTA in AR. The concentrations of OTA in 187 samples of AR were investigated, and 61 (32.6%) samples were positive. The mean, 50th and 95th percentile values of OTA in positive samples were 56.2, 5.1 and 304.5 μg/kg, respectively. A margin of exposure (MOE) approach was applied to assess the risk. Considering other food sources, long-term consumers have a relatively high risk of OTA exposure due to the ingestion of AR. Theoretical limit levels of OTA in AR were evaluated from two dimensions by weighing the costs and the benefits. The results indicated that the limit levels that might be applied to the management of OTA contamination in AR in China could be screened out through risk-based evaluation of limit levels.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113517DOI Listing
June 2022

Dynamics of phytoplankton community in scallop farming waters of the Bohai Sea and North Yellow Sea in China.

BMC Ecol Evol 2022 Apr 15;22(1):48. Epub 2022 Apr 15.

Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.

Background: As the major suppliers of food for higher consumers, phytoplankton are closely related to the yield, nutritional ingredients and even toxin contents of mariculture animals, potentially influencing the human health when they are consumed. With the increase of shellfish culture density, phytoplankton in the coastal waters have been excessively consumed in recent years, and the nutrients they depend on are becoming more and more limited, which severely restrict the shellfish mariculture and threaten the coastal ecosystems.

Results: In the present study, nutrient concentrations, phytoplankton assemblages and scallop growth status were investigated in the main bay scallop farming waters of the Bohai Sea (Qinhuangdao site) and North Yellow Sea (Zhuanghe site) in 2018. Both phosphate and silicate limitations were observed at the two sites, with the major determinant of phytoplankton assemblages being silicate in Qinhuangdao and phosphate in Zhuanghe, respectively. The phytoplankton assemblages at the two sites displayed different community structures and succession patterns. The phytoplankton community was dominated by dinoflagellates and diatoms in Qinhuangdao, while dinoflagellates were the most abundant group in Zhuanghe, which accounted for 41.9% of the total phytoplankton abundance. The dominant genera of diatoms in Qinhuangdao were Skeletonema, Thalassiosira and Leptocylindrus, while those in Zhuanghe were Thalassiosira and Cyclotella. Greater biomass and more appropriate structure of phytoplankton contributed to higher growth rate and glycogen content of cultured bay scallops.

Conclusions: Our study characterized the relationship between nutrient concentration, phytoplankton community and scallop mariculture in the main bay scallop farming waters in northern China. The results suggest that, as nutrient limitation intensified, dinoflagellates are becoming the dominant phytoplankton species in the scallop farming waters of the Bohai Sea and the North Yellow Sea, which is harmful to the coastal mariculture.
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http://dx.doi.org/10.1186/s12862-022-02002-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013024PMC
April 2022

Cigarette Smoke or Motor Vehicle Exhaust Exposure Induces PD-L1 Upregulation in Lung Epithelial Cells in COPD Model Rats.

COPD 2022 ;19(1):206-215

Department of Respiratory Medicine, The First School of Clinical Medicine, Southern Medical University (General Hospital of Southern Theater Command of PLA), Guangzhou, PR China.

A high smoking-independent chronic obstructive pulmonary disease (COPD) prevalence is observed in lung cancer patients. However, the underlying connection between these two diseases still remains unclear. Cigarette smoking and ambient air pollution are common risk factors for COPD and lung cancer. In this study, we established rat COPD model through exposure to cigarette smoke (CS) or motor vehicle exhaust (MVE). The model rats developed COPD-like phenotypes, manifested as lung functions decline, lung inflammation, emphysema-like alveolar enlargement and airway remodeling. The programmed death-ligand 1 (PD-L1), a factor contributing to immune escape of tumor cells, was overexpressed in lungs from COPD model rats, though more severe COPD phenotypes did not bring with further PD-L1 overexpression in lung. The upregulations of proinflammatory cytokines and PD-L1 were also observed in cultured human bronchial epithelial cells BEAS-2B upon treatment with cigarette smoke extract (CSE) or diesel-related particulate matter 2.5 (PM, SEM1650b). The inflammatory cytokines produced in BEAS-2B cells reflected the PD-L1 levels. Furthermore, ERK1/2, a kinase mediating PD-L1 upregulation in premalignant bronchial cells or NSCLC cells, and STAT1/3, which was reportedly associated with PD-L1 expression in lung tumors, were activated in COPD rats' lungs or in BEAS-2B cells treated with CSE or PM. Therefore, we proposed that inflammation associated PD-L1 overexpression in airway epithelial cells could be the underlying factor facilitating lung cancer incidence in COPD.
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http://dx.doi.org/10.1080/15412555.2022.2058924DOI Listing
January 2022

Excitation of Surface Plasmon Polariton Modes with Double-Layer Gratings of Graphene.

Nanomaterials (Basel) 2022 Mar 30;12(7). Epub 2022 Mar 30.

Laboratory for Micro-Nano Physics and Technology of Hunan Province, School of Physics and Electronics, Hunan University, Changsha 410082, China.

A long-range surface plasmon polariton (SPP) waveguide, composed of double-layer graphene, can be pivotal in transferring and handling mid-infrared electromagnetic waves. However, one of the key challenges for this type of waveguide is how to excite the SPP modes through an incident light beam. In this study, our proposed design of a novel grating, consisting of a graphene-based cylindrical long-range SPP waveguide array, successfully addresses this issue using finite-difference time-domain simulations. The results show that two types of symmetric coupling modes (SCMs) are excited through a normal incident light. The transmission characteristics of the two SCMs can be manipulated by changing the interaction of the double-layer gratings of graphene as well as by varying various parameters of the device. Similarly, four SCMs can be excited and controlled by an oblique incident light because this light source is equivalent to two orthogonal beams of light. Furthermore, this grating can be utilized in the fabrication of mid-infrared optical devices, such as filters and refractive index sensors. This grating, with double-layer graphene arrays, has the potential to excite and manipulate the mid-infrared electromagnetic waves in future photonic integrated circuits.
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http://dx.doi.org/10.3390/nano12071144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000374PMC
March 2022

Characterizing the stabilization effects of stabilizers in protein-protein systems with end-point binding free energy calculations.

Brief Bioinform 2022 May;23(3)

Department of Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, Jiangsu, P. R. China.

Drug design targeting protein-protein interactions (PPIs) associated with the development of diseases has been one of the most important therapeutic strategies. Besides interrupting the PPIs with PPI inhibitors/blockers, increasing evidence shows that stabilizing the interaction between two interacting proteins may also benefit the therapy, such as the development of various types of molecular glues/stabilizers that mostly work by stabilizing the two interacting proteins to regulate the downstream biological effects. However, characterizing the stabilization effect of a stabilizer is usually hard or too complicated for traditional experiments since it involves ternary interactions [protein-protein-stabilizer (PPS) interaction]. Thus, developing reliable computational strategies will facilitate the discovery/design of molecular glues or PPI stabilizers. Here, by fully analyzing the energetic features of the binary interactions in the PPS ternary complex, we systematically investigated the performance of molecular mechanics Poisson-Boltzmann surface area (MM/PBSA) and molecular mechanics generalized Born surface area (MM/GBSA) methods on characterizing the stabilization effects of stabilizers in 14-3-3 systems. The results show that both MM/PBSA and MM/GBSA are powerful tools in distinguishing the stabilizers from the decoys (with area under the curves of 0.90-0.93 for all tested cases) and are reasonable for ranking protein-peptide interactions in the presence or absence of stabilizers as well (with the average Pearson correlation coefficient of ~0.6 at a relatively high dielectric constant for both methods). Moreover, to give a detailed picture of the stabilization effects, the stabilization mechanism is also analyzed from the structural and energetic points of view for individual systems containing strong or weak stabilizers. This study demonstrates a potential strategy to accelerate the discovery of PPI stabilizers.
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http://dx.doi.org/10.1093/bib/bbac127DOI Listing
May 2022

Electrocleavage Synthesis of Solution-Processed, Imine-Linked, and Crystalline Covalent Organic Framework Thin Films.

J Am Chem Soc 2022 Apr 5. Epub 2022 Apr 5.

State Key Laboratory of Luminescent Materials and Devices, Institute of Polymer Optoelectronic Materials and Devices, South China University of Technology, Guangzhou 510640, P. R. China.

Developing a general, facile, and direct strategy for synthesizing thin films of covalent organic frameworks (COFs) is a major challenge in this field. Herein, we report an unprecedented electrocleavage synthesis strategy to produce imine-linked COF films directly on electrodes from electrolyte solutions at room temperature. This strategy enables the cathodic exfoliation of the COF powders to nanosheets by electrochemical reduction and protonation, followed by nanosheets migrating to the anode and reproducing the COF structures by anodic oxidation. Our method is adaptable with most imine-linked COFs by virtue of the low redox potential of the imine bonds, whereas the COF films possess high crystallinity and hierarchical porosity. We highlight these COF films as a superb platform for promoting mass transfer by demonstrating their extraordinarily rapid iodine adsorption with record-high rate constants.
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http://dx.doi.org/10.1021/jacs.1c13072DOI Listing
April 2022

Enhanced computed tomography imaging features predict tumor grade in pseudomyxoma peritonei.

Quant Imaging Med Surg 2022 Apr;12(4):2321-2331

Department of Radiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

Background: Because few studies have focused on the correlation between computed tomography (CT) signs and tumor grade in pseudomyxoma peritonei (PMP), we evaluated predictive value of abdominal enhanced CT in identifying high- . low-grade cases.

Methods: In all, 75 patients diagnosed with PMP after surgery were consecutively recruited. The preoperative enhanced CT images were retrospectively analyzed for ascites, hepatic scalloping, omental and peritoneal lesion appearance, intralesional calcification and septa, and peripheral organ involvement. Logistic regression models were applied to analyze the relationship of CT signs with PMP grade. Receiver operating characteristic curves were generated to evaluate the potential utility of CT signs in detecting high-grade PMP.

Results: Massive ascites (P=0.017) and peritoneal solid nodules (P<0.001) were more common in high-grade cases. Multivariate logistic regression identified massive ascites [odds ratio (OR) =4.389, 95% confidence interval (CI): 1.210-15.921; P=0.025] and peritoneal solid nodules (OR =19.932, 95% CI: 3.560-111.596; P<0.001) as independent predictors of high-grade PMP. For the 55 patients with hepatic scalloping, the maximum thickness of mucin deposition at the hepatic scalloping wave in high-grade PMP was thinner than that in low-grade PMP (P=0.021). Thickness of mucin deposition at the hepatic scalloping wave (OR =0.346, 95% CI: 0.148-0.809; P=0.014) was an independent predictor of high-grade PMP, with a cutoff value of 18.6 mm. Cancer antigen 125 (CA125) combined with CT signs was significantly better at diagnosing high-grade PMP than was CA125 alone in both the overall patients [area under the ROC curve (AUC): 0.812 . 0.656; P=0.020] and those with hepatic scalloping (AUC: 0.859 . 0.600; P=0.007).

Conclusions: The CT signs of high-grade PMP significantly differ from those of low-grade PMP, and thus combining CT signs with CA125 may be highly valuable for classifying PMP.
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http://dx.doi.org/10.21037/qims-21-976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8923854PMC
April 2022

Evaluation of the Effect of Comprehensive Nursing Interventions on Plaque Control in Patients with Periodontal Disease in the Context of Artificial Intelligence.

J Healthc Eng 2022 23;2022:6505672. Epub 2022 Mar 23.

Department of Stomatology, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050017, Hebei, China.

Plaque is a bacterial biofilm that adheres to each other and exists on the tooth surface, and new plaque can continuously reform after removing it from the tooth surface. The pathogenesis of periodontal disease is related to the bacteria, the host and the environment, with the bacteria and bacterial products in plaque being the main initiators of periodontal disease. The effective control of plaque is an effective method for the treatment and prevention of periodontal disease and is often underappreciated in clinical practice. For the traditional diagnostic method through experience and visual observation, it may lead to misdiagnosis and underdiagnosis. In order to accurately diagnose plaque disease, this study designed a convolutional neural network-based oral dental disease diagnosis system for oral care interventions to improve oral health awareness. Thus motivate patients to implement proper oral health care measures, and continuously and lifelong insist on thorough daily plaque removal to improve patients' physical health and quality of life in periodontal disease patients.
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http://dx.doi.org/10.1155/2022/6505672DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967516PMC
April 2022

A model to predict a risk of allergic rhinitis based on mitochondrial DNA copy number.

Eur Arch Otorhinolaryngol 2022 Mar 29. Epub 2022 Mar 29.

Department of Otolaryngology, Xinjiang Medical University Affiliated First Hospital, 137 Liyushan Avenue, Xinshi District, Urumqi, 830054, Xinjiang, China.

Purpose: To determine whether mitochondrial DNA copy number (mtDNA-CN) is associated with allergic rhinitis (AR), and further establish a nomogram model for the early diagnosis of AR.

Methods: We carried out a case-control study involving a total of 134 subjects, including 66 healthy controls and 68 AR patients. The mtDNA-CN in peripheral blood of all subjects was detected by real-time fluorescence quantitative polymerase chain reaction, and general information of patients was recorded. And, least absolute shrinkage selection operator (LASSO) regression was used to screen clinically significant variables, which were substituted into a logistic regression analysis to determine independent risk factors. Next, a nomogram model was developed for the risk prediction of AR. Then, internal validation was performed with the bootstrap resampling. Ultimately, the clinical benefit and validity of the nomogram were assessed by receiver operating characteristic (ROC) curve, bias-corrected curve, and decision curve analysis (DCA).

Results: MtDNA-CN and total IgE were determined as independent risk factors of AR. The final model achieved an area under the ROC curve (AUC) of 0.869, and the DCA curve demonstrated that the nomogram was clinically beneficial for practical application.

Conclusion: An increase of the mtDNA-CN was linked to the occurrence risk of AR. The nomogram prediction model based on mtDNA-CN showed the potential clinical utility in improving risk prediction and providing new insights for exploring the pathogenesis of AR.
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http://dx.doi.org/10.1007/s00405-022-07341-7DOI Listing
March 2022

The metabolism and biotransformation of AFB: Key enzymes and pathways.

Biochem Pharmacol 2022 05 19;199:115005. Epub 2022 Mar 19.

Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, Guangdong, PR China; Key Laboratory of Zoonosis of the Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. Electronic address:

Aflatoxins B (AFB) is a hepatoxic compound produced by Aspergillus flavus and Aspergillus parasiticus, seriously threatening food safety and the health of humans and animals. Understanding the metabolism of AFB is important for developing detoxification and intervention strategies. In this review, we summarize the AFB metabolic fates in humans and animals and the key enzymes that metabolize AFB, including cytochrome P450s (CYP450s) for AFB bioactivation, glutathione-S-transferases (GSTs) and aflatoxin-aldehyde reductases (AFARs) in detoxification. Furthermore, AFB metabolism in microbes is also summarized. Microorganisms specifically and efficiently transform AFB into less or non-toxic products in an environmental-friendly approach which could be the most desirable detoxification strategy in the future. This review provides a wholistic insight into the metabolism and biotransformation of AFB in various organisms, which also benefits the development of protective strategies in humans and animals.
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http://dx.doi.org/10.1016/j.bcp.2022.115005DOI Listing
May 2022

Role of hydrogen sulfide in subarachnoid hemorrhage.

CNS Neurosci Ther 2022 06 22;28(6):805-817. Epub 2022 Mar 22.

Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China.

Subarachnoid hemorrhage (SAH) is a common acute and severe disease worldwide, which imposes a heavy burden on families and society. However, the current therapeutic strategies for SAH are unsatisfactory. Hydrogen sulfide (H S), as the third gas signaling molecule after carbon monoxide and nitric oxide, has been widely studied recently. There is growing evidence that H S has a promising future in the treatment of central nervous system diseases. In this review, we focus on the effects of H S in experimental SAH and elucidate the underlying mechanisms. We demonstrate that H S has neuroprotective effects and significantly reduces secondary damage caused by SAH via antioxidant, antiinflammatory, and antiapoptosis mechanisms, and by alleviating cerebral edema and vasospasm. Based on these findings, we believe that H S has great potential in the treatment of SAH and warrants further study to promote its early clinical application.
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http://dx.doi.org/10.1111/cns.13828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062544PMC
June 2022

Enhancing S-nitrosoglutathione reductase decreases S-nitrosylation of Drp1 and reduces neuronal apoptosis in experimental subarachnoid hemorrhage both in vivo and in vitro.

Brain Res Bull 2022 Jun 15;183:184-200. Epub 2022 Mar 15.

Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China; Institute of Stroke Research, Soochow University, Suzhou 215006, China. Electronic address:

Subarachnoid hemorrhage (SAH) is a hemorrhagic stroke with a high mortality and disability rate. Nitric oxide (NO) can promote blood supply through vasodilation, leading to protein S-nitrosylation. However, the function of S-nitrosylation in neurons after SAH remains unclear. Excessive NO in the pathological state is converted into S-nitrosoglutathione (GSNO) and stored in cells, which leads to high S-nitrosylation of intracellular proteins and causes nitrosative stress. S-nitrosoglutathione reductase (GSNOR) promotes GSNO degradation and protects cells from excessive S-nitrosylation. We conducted an in vivo rat carotid puncture model and an in vitro neuron hemoglobin intervention. The results showed that SAH induction increased NO, GSNO, neuron protein S-nitrosylation, and neuronal apoptosis, while decreasing the level and activity of GSNOR. GSNOR overexpression by lentivirus decreased GSNO but had little effect on NO. GSNOR overexpression also improved short- and long-term neurobehavioral outcomes in rats and alleviated nitrosative stress. Furthermore, GSNOR reduced neuronal apoptosis and played a neuroprotective role by alleviating Drp1 S-nitrosylation, reducing mitochondrial division. Thus, the regulation of GSNOR in early brain injury and neuronal denitrosylation may play an important role in neuroprotection.
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http://dx.doi.org/10.1016/j.brainresbull.2022.03.010DOI Listing
June 2022
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