Publications by authors named "Ling Zhao"

998 Publications

Emergence of IncX3 Plasmid-Harboring in a Isolated from Outdoor Aerosol in Wastewater Treatment Plant.

Microb Drug Resist 2021 Sep 13. Epub 2021 Sep 13.

Department of Environment and Health, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, China.

A carbapenem-resistant strain AA2CS carrying was detected in outdoor aerosols of a wastewater treatment plant (WWTP) in China and the whole genome was sequenced subsequently. AA2CS was captured in an aerobic tank with aerosol particles of sizes ranging from 4.7 to 7.0 μm. Besides , AA2CS also harbored 21 other antibiotic resistance genes and displayed a high level of resistance to ampicillin, cefotaxime, ceftazidime, tetracycline, and meropenem. was located on the IncX3 plasmid (pCSNDM-5) with an IS-IS5-----IS structure. pCSNDM-5 was highly homologous to other -carrying IncX3 plasmids in China and can be transferred to the recipient J53. To our knowledge, this is the first report of carbapenem-resistant Enterobacteriaceae in outdoor aerosols in WWTPs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/mdr.2021.0057DOI Listing
September 2021

Targeting CA-125 Transcription by Development of a Conditionally Replicative Adenovirus for Ovarian Cancer Treatment.

Cancers (Basel) 2021 Aug 24;13(17). Epub 2021 Aug 24.

Department of Medical Oncology and Therapeutics Research, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, CA 91010, USA.

CA-125, encoded by the gene, is highly expressed in most ovarian cancer cells and thus serves as a tumor marker for monitoring disease progression or treatment response in ovarian cancer patients. However, targeting /CA-125 for ovarian cancer treatment has not been successful to date. In the current study, we performed multiple steps of high-fidelity PCR and obtained a 5 kb DNA fragment upstream of the human gene. Reporter assays indicate that this DNA fragment possesses transactivation activity in CA-125-high cancer cells, but not in CA-125-low cancer cells, indicating that the DNA fragment contains the transactivation region that controls specific expression of the gene in ovarian cancer cells. We further refined the promoter and found a 1040 bp fragment with similar transcriptional activity and specificity. We used this refined promoter to replace the E1A promoter in the adenovirus type 5 genome DNA, where E1A is an essential gene for adenovirus replication. We then generated a conditionally replicative oncolytic adenovirus (CRAd) that replicates in and lyses CA-125-high cancer cells, but not CA-125-low or -negative cancer cells. In vivo studies showed that intraperitoneal virus injection prolonged the survival of NSG mice inoculated intraperitoneally (ip) with selected ovarian cancer cell lines. Furthermore, the CRAd replicates in and lyses primary ovarian cancer cells, but not normal cells, collected from ovarian cancer patients. Collectively, these data indicate that targeting transactivation utilizing CRAd is a feasible approach for ovarian cancer treatment that warrants further investigation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/cancers13174265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428227PMC
August 2021

Colloidal manganese salt improves the efficacy of rabies vaccines in mice, cats, and dogs.

J Virol 2021 Sep 8:JVI0141421. Epub 2021 Sep 8.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.

Rabies, caused by rabies virus (RABV), remains a serious threat to public health in most countries worldwide. At present, the administration of rabies vaccines has been the most effective strategy to control rabies. Herein, we evaluate the effect of colloidal manganese salt (Mn jelly, MnJ) as an adjuvant of rabies vaccine in mice, cats, and dogs. The results showed that MnJ promoted type I interferon (IFN-I) and cytokine production and the maturation of dendritic cells (DCs) and . Besides, MnJ serving as an adjuvant for rabies vaccines could significantly facilitate the generation of T follicular helper (Tfh) cells, germinal center (GC) B cells, plasma cells (PCs), and RABV-specific antibody-secreting-cells (ASCs), consequently improved the immunogenicity of rabies vaccines and provide better protection against virulent RABV challenge. Similarly, MnJ enhanced the humoral immune response in cats and dogs as well. Collectively, our results suggest that MnJ can facilitate the maturation of DCs during rabies vaccination, which can be a promising adjuvant candidate for rabies vaccines. Extending humoral immune response by using adjuvants is an important strategy for vaccine development. In this study, a novel adjuvant MnJ supplemented in rabies vaccines was evaluated in mice, cats, and dogs. Our results in the mouse model revealed that MnJ increased the numbers of mature DCs, Tfh cells, GC B cells, PCs, and RABV-specific ASCs, resulting in enhanced immunogenicity and protection rate of rabies vaccines. We further found MnJ had the same stimulative effect in cats and dogs. Our study provides the first evidence that MnJ serving as a novel adjuvant of rabies vaccines can boost immune response both in a mouse and pet model.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1128/JVI.01414-21DOI Listing
September 2021

Migration and transformation of chromium in unsaturated soil during groundwater table fluctuations induced by rainfall.

J Hazard Mater 2021 Aug 26;416:126229. Epub 2021 May 26.

School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Engineering Research Center of Solid Waste Treatment and Resource Recovery, Shanghai Jiao Tong University, Shanghai 200240, China.

The groundwater table fluctuation zone is the main interface for contaminants to transport between the unsaturated soil and saturated aquifers which still lacks of concern. In this study, we explored the interactions of Cr(VI) in this specific zone during water table fluctuation through laboratory experiment and numerical modeling. The higher reduction of Cr(VI) was found in the lower soil layer due to the lower Eh at the bottom layer of the unsaturated zone and the Cr(III) concentration increased with rise in water level and fluctuation amplitudes. After twice water fluctuation, nevertheless, there was still about 42.2% Cr retained in the soil and dominantly present as Cr(III) form. The model coupling reaction network with hydrodynamic field showed the cumulative Cr(III) in the unsaturated soil zone had a faster increase at the higher water level rise speed compared with lower rise speed. The cumulative Cr(VI) decreases over time in the saturated aquifers, whereas the cumulative Cr(III) increased with the increase of fluctuation amplitude. Reduction of Cr(VI) into Cr(III) was accompanied with Fe(II) and organic carbon oxidation. The results indicate that the hydrodynamic conditions have impacts on the redox environment of soil which could further affect the transformation and transport of Cr.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.126229DOI Listing
August 2021

Cu-induced spermatogenesis disease is related to oxidative stress-mediated germ cell apoptosis and DNA damage.

J Hazard Mater 2021 08 16;416:125903. Epub 2021 Apr 16.

College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.

Copper is considered as an indispensable trace element for living organisms. However, over-exposure to Cu can lead to adverse health effects on human. In this study, CuSO decreased sperm concentration and motility, increased sperm malformation rate. Concurrently, testicular damage including testicular histopathological aberrations and reduction of testis relative weight were observed. Then, the mechanism underlying Cu-induced testicular toxicity was explored. According to the results, CuSO elevated ROS production while reducing antioxidant function. Additionally, CuSO induced apoptosis which was featured by MMP depolarization and up-regulated levels of cleaved-caspase-3, cleaved-caspase-8, cleaved-caspase-9, caspase-12, cleaved-PARP and Bax, whereas down-regulated Bcl-2 expression. Meanwhile, CuSO caused testis DNA damage (up-regulation of γ-H2AX protein expression) and suppressed DNA repair pathways including BER, NER, HR, MMR, together with the NHEJ repair pathways, yet did not affect MGMT. To investigate the role of oxidative stress in CuSO-induced apoptosis and DNA damage, the antioxidant NAC was co-treated with CuSO. NAC attenuated CuSO-induced ROS production, inhibited apoptosis and DNA damage. Furthermore, the spermatogenesis disorder was also abolished in the co-treatment with CuSO and NAC group. Altogether, abovementioned results indicated that CuSO-induced spermatogenesis disorder is related to oxidative stress-mediated DNA damage and germ cell apoptosis, impairing male reproductive function.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.125903DOI Listing
August 2021

Preexposure and Postexposure Prophylaxis of Rabies With Adeno-Associated Virus Expressing Virus-Neutralizing Antibody in Rodent Models.

Front Microbiol 2021 19;12:702273. Epub 2021 Aug 19.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.

Rabies, a fatal disease in humans and other mammals, is caused by the rabies virus (RABV), and it poses a public health threat in many parts of the world. Once symptoms of rabies appear, the mortality is near 100%. There is currently no effective treatment for rabies. In our study, two human-derived RABV-neutralizing antibodies (RVNA), CR57 and CR4098, were cloned into adeno-associated virus (AAV) vectors, and recombinant AAVs expressing RVNA were evaluated for postexposure prophylaxis after intrathecal injection into RABV-infected rats. At 4days post-infection with a lethal dose of RABV, 60% of the rats that received an intrathecal injection of AAV-CR57 survived, while 100% of the rats inoculated with AAV-enhanced green fluorescent protein (EGFP) succumbed to rabies. Overall, these results demonstrate that AAV-encoding RVNA can be utilized as a potential human rabies postexposure prophylaxis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.702273DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417364PMC
August 2021

Direct Measurement of the Branching Fractions B(ψ(3686)→J/ψX) and B(ψ(3770)→J/ψX), and Observation of the State R(3760) in e^{+}e^{-}→J/ψX.

Phys Rev Lett 2021 Aug;127(8):082002

Istanbul Bilgi University, 34060 Eyup, Istanbul, Turkey.

We report a measurement of the observed cross sections of e^{+}e^{-}→J/ψX based on 3.21  fb^{-1} of data accumulated at energies from 3.645 to 3.891 GeV with the BESIII detector operated at the BEPCII collider. In analysis of the cross sections, we measured the decay branching fractions of B(ψ(3686)→J/ψX)=(64.4±0.6±1.6)% and B(ψ(3770)→J/ψX)=(0.5±0.2±0.1)% for the first time. The energy-dependent line shape of these cross sections cannot be well described by two Breit-Wigner (BW) amplitudes of the expected decays ψ(3686)→J/ψX and ψ(3770)→J/ψX. Instead, it can be better described with one more BW amplitude of the decay R(3760)→J/ψX. Under this assumption, we extracted the R(3760) mass M_{R(3760)}=3766.2±3.8±0.4  MeV/c^{2} , total width Γ_{R(3760)}^{tot}=22.2±5.9±1.4  MeV, and product of leptonic width and decay branching fraction Γ_{R(3760)}^{ee}B[R(3760)→J/ψX]=(79.4±85.5±11.7)  eV. The significance of the R(3760) is 5.3σ. The first uncertainties of these measured quantities are from fits to the cross sections and second systematic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1103/PhysRevLett.127.082002DOI Listing
August 2021

Hepatic artery injection of I-metuximab combined with transcatheter arterial chemoembolization for unresectable hepatocellular carcinoma: a prospective non-randomized, multicenter clinical trial.

J Nucl Med 2021 Sep 2. Epub 2021 Sep 2.

College of Military Preventive Medicine, Fourth Military Medical University,, China.

This prospective non-randomized, multicenter clinical trial was performed to investigate efficacy and safety of I-labeled metuximab in adjuvant treatment of unresectable hepatocellular carcinoma. Patients were assigned to treatment with transcatheter arterial chemoembolization (TACE) combined with I-metuximab or TACE alone. The primary outcome was overall tumor recurrence. The secondary outcomes were safety and overall survival. The median time to tumor recurrence was 6 months in the TACE+I-metuximab group ( = 160) and 3 months in the TACE group ( = 160) (hazard ratio, 0.55; 95% confidence interval, 0.43 to 0.70; < 0.001). The median overall survival was 28 months in the TACE+I-metuximab group and 19 months in the TACE group (hazard ratio, 0.62; 95% confidence interval, 0.47 to 0.82; = 0.001). TACE+I-metuximab showed a greater anti-recurrence benefit, significantly improved the 5-year survival of patients with advanced hepatocellular carcinoma, and was well tolerated by patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2967/jnumed.121.262136DOI Listing
September 2021

Further reuse of phosphorus-laden biochar for lead sorption from aqueous solution: Isotherm, kinetics, and mechanism.

Sci Total Environ 2021 Oct 17;792:148550. Epub 2021 Jun 17.

School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

Biochar and engineered biochar have been used for phosphorous recovery from wastewater, but the resulted phosphorous-laden (P-laden) biochar needs further disposal. In this study, the feasibility of reusing P-laden biochar for Pb immobilization as well as the underlying mechanism was explored. Three types of engineered biochar, i.e., Ca modified biochar, Mg modified biochar, and Fe modified biochar, were selected to sorb P and then the exhausted biochar was further used for Pb sorption. Results showed that Mg and Ca modified biochar exhibited considerable Pb sorption capacity after P sorption with the maximum value of 3.36-4.03 mmol/g and 5.49-6.58 mmol/g, respectively, while P-laden Fe modified biochar failed to sorb Pb due to its acidic pH. The removal of Pb by P-laden Mg modified biochar involved more precipitation including PbHPO, Pb(PO)(OH), and Pb(CO)(OH) because of its higher P sorption capacity and more -OH group on the surface. Cation exchange with CaCO to form PbCO was the main mechanism for Pb removal by P-laden Ca modified biochar despite the formation of Pb(PO)(OH) precipitate. Our results demonstrate that waste P-laden biochar can be further used for the effective removal of Pb, which provides a potential approach for waste adsorbent disposal.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.148550DOI Listing
October 2021

Analysis of Bubble Growth in Supercritical CO Extrusion Foaming Polyethylene Terephthalate Process Based on Dynamic Flow Simulation.

Polymers (Basel) 2021 Aug 20;13(16). Epub 2021 Aug 20.

Shanghai Key Laboratory of Multiphase Materials Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China.

Bubble growth in the polymer extrusion foaming process occurs under a dynamic melt flow. For non-Newtonian fluids, this work successfully coupled the dynamic melt flow simulation with the bubble growth model to realize bubble growth predictions in an extrusion flow. The initial thermophysical properties and dynamic rheological property distribution at the cross section of the die exit were calculated based on the finite element method. It was found that dynamic rheological properties provided a necessary solution for predicting bubble growth during the supercritical CO polyethylene terephthalate (PET) extrusion foaming process. The introduction of initial melt stress could effectively inhibit the rapid growth of bubbles and reduce the stable size of bubbles. However, the initial melt stress was ignored in previous work involving bubble growth predictions because it was not available. The simulation results based on the above theoretical model were consistent with the evolution trends of cell morphology and agreed well with the actual experimental results.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym13162799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401983PMC
August 2021

Facile Synthesis of Thermoplastic Polyamide Elastomers Based on Amorphous Polyetheramine with Damping Performance.

Polymers (Basel) 2021 Aug 9;13(16). Epub 2021 Aug 9.

State Key Laboratory of Chemical Engineering, School of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China.

Novel thermoplastic polyamide elastomers (TPAEs) consisting of long-chain semicrystalline polyamide 1212 (PA1212) and amorphous polyetheramine were synthesized via one-pot melt polycondensation. The method provides accessible routes to prepare TPAEs with a high tolerance of compatibility between polyamide and polyether oligomers compared with the traditional two-step method. These TPAEs with 10 wt % to 76 wt % of soft content were obtained by reaction of dodecanedioic acid, 1,12-dodecanediamine, and poly(propylene glycol) (PPG) diamine. The structure-property relationships of TPAEs were systematically studied. The chemical structure and the morphologic analyses have revealed that microphase separation occurs in the amorphous region. The TPAEs that have long-chain PPG segments consist of a crystalline polyamide domain, amorphous polyamide-rich domain, and amorphous polyetheramine-rich domain, while the ones containing short-chain PPG segments comprise of a crystalline polyamide domain and miscible amorphous polyamide phase and amorphous polyetheramine phase due to the compatibility between short-chain polyetheramine and amorphous polyamide. These novel TPAEs show good damping performance at low temperature, especially the TPAEs that incorporated 76 wt % and 62 wt % of PPG diamine. The TPAEs exhibit high elastic properties and low residual strain at room temperature. They are lightweight with density between 1.01 and 1.03 g/cm. The long-chain TPAEs have well-balanced properties of low density, high elastic return, and high shock-absorbing ability. This work provides a route to expand TPAEs to damping materials with special application for sports equipment used in extremely cold conditions such as ski boots.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym13162645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397970PMC
August 2021

The Time-Dependence of Three Different Modes of ELF-EMF Stimulation on LTP at Schaffer Collateral-CA1 Synapses.

Bioelectromagnetics 2021 Oct 27;42(7):538-549. Epub 2021 Aug 27.

School of Life Sciences, Tiangong University, Tianjin, China.

Long-term potentiation (LTP) is considered the cellular basis of learning and memory. Extremely low-frequency electromagnetic fields (ELF-EMFs) are neuromodulation tools for regulating LTP. However, the temporal effects of short-term ELF-EMF stimulation on LTP are not yet known. In this study, we evaluated the time-dependent effects of 15 Hz/2 mT ELF-EMF stimulation on LTP at the Schaffer collateral-CA1 (SC-CA1) synapses in Sprague-Dawley rats. Hippocampal slices were exposed to three different modes of ELF-EMFs (sinusoidal, single-frequency pulse, and rhythm pulse) and durations (10, 20, 40, and 60 s). The baseline was recorded for 20 min and field excitatory postsynaptic potential (fEPSP) was recorded for 60 min using multi-electrode arrays (MEA) after plasticity induction using 100 Hz electrical high-frequency stimulation (HFS). Compared to the control group, the LTP decreased under three different magnetic fields and was proportional to time; that is, the longer the time, the greater the inhibition. We also compared the three magnetic fields and showed that the continuous sinusoidal magnetic field had the largest inhibitory rate of LTP, while pulsed and rhythm pulsed magnetic fields were similar. We showed that different modes of ELF-EMF stimulation had a time-dependent effect on LTP at Schaffer collateral-CA1 synapses, which provides experimental evidence for the treatment of related neurological diseases. © 2021 Bioelectromagnetics Society.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/bem.22369DOI Listing
October 2021

Ultra-fast single-crystal polymerization of large-sized covalent organic frameworks.

Nat Commun 2021 Aug 23;12(1):5077. Epub 2021 Aug 23.

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, China.

In principle, polymerization tends to produce amorphous or poorly crystalline materials. Efficiently producing high-quality single crystals by polymerization in solvent remains as an unsolved issue in chemistry, especially for covalent organic frameworks (COFs) with highly complex structures. To produce μm-sized single crystals, the growth time is prolonged to >15 days, far away from the requirements in practical applications. Here, we find supercritical CO (sc-CO) accelerates single-crystal polymerization by 10,000,000 folds, and produces two-dimensional (2D) COF single crystals with size up to 0.2 mm within 2~5 min. Although it is the fastest single-crystal polymerization, the growth in sc-CO leads to not only the largest crystal size of 2D COFs, but also higher quality with improved photoconductivity performance. This work overcomes traditional concept on low efficiency of single-crystal polymerization, and holds great promise for future applications owing to its efficiency, industrial compatibility, environmental friendliness and universality for different crystalline structures and linkage bonds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-24842-xDOI Listing
August 2021

Macrophage-derived extracellular vesicles regulates USP5-mediated HDAC2/NRF2 axis to ameliorate inflammatory pain.

FASEB J 2021 09;35(9):e21332

Department of Rheumatology, The First Hospital of Jilin University, Changchun, P.R. China.

Emerging research has highlighted the capacity of microRNA-23a-3p (miR-23a-3p) to alleviate inflammatory pain. However, the molecular mechanism by which miR-23a-3p attenuates inflammatory pain is yet to be fully understood. Hence, the current study aimed to elucidate the mechanism by which miR-23a-3p influences inflammatory pain. Bioinformatics was initially performed to predict the inflammatory pain related downstream targets of miR-23a-3p in macrophage-derived extracellular vesicles (EVs). An animal inflammatory pain model was established using Complete Freund's Adjuvant (CFA). The miR-23a-3p expression was downregulated in the microglia of CFA-induced mice, after which the inflammatory factors were determined by ELISA. FISH and immunofluorescence were performed to analyze the co-localization of miR-23a-3p and microglia. Interestingly, miR-23a-3p was transported to the microglia via M2 macrophage-EVs, which elevated the mechanical allodynia and the thermal hyperalgesia thresholds in mice model. The miR-23a-3p downstream target, USP5, was found to stabilize HDAC2 via deubiquitination to promote its expression while inhibiting the expression of NRF2. Taken together, the key findings of the current study demonstrate that macrophage-derived EVs containing miR-23a-3p regulates the HDAC2/NRF2 axis by decreasing USP5 expression to alleviate inflammatory pain, which may provide novel therapeutic targets for the treatment of inflammatory pain.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.202001185RRDOI Listing
September 2021

Paeonol Attenuates Quorum-Sensing Regulated Virulence and Biofilm Formation in .

Front Microbiol 2021 4;12:692474. Epub 2021 Aug 4.

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

With the prevalence of multidrug-resistant bacteria and clinical -acquired pathogenic infections, the development of quorum-sensing (QS) interfering agents is one of the most potential strategies to combat bacterial infections and antibiotic resistance. Chinese herbal medicines constitute a valuable bank of resources for the identification of QS inhibitors. Accordingly, in this research, some compounds were tested for QS inhibition using indicator strains. Paeonol is a phenolic compound, which can effectively reduce the production of violacein without affecting its growth in ATCC 12472, indicating its excellent anti-QS activity. This study assessed the anti-biofilm activity of paeonol against Gram-negative pathogens and investigated the effect of paeonol on QS-regulated virulence factors in . A infection model was used to explore the anti-infection ability of paeonol . Paeonol exhibited an effective anti-biofilm activity against Gram-negative bacteria. The ability of paeonol to interfere with the AHL-mediated quorum sensing systems of was determined, found that it could attenuate biofilm formation, and synthesis of pyocyanin, protease, elastase, motility, and AHL signaling molecule in a concentration- and time-dependent manner. Moreover, paeonol could significantly downregulate the transcription level of the QS-related genes of including , as well as mediated its virulence factors, , and . studies revealed that paeonol could reduce the pathogenicity of and enhance the survival rate of , showing a moderate protective effect on . Collectively, these findings suggest that paeonol attenuates bacterial virulence and infection of and that further research elucidating the anti-QS mechanism of this compound is warranted.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.692474DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371487PMC
August 2021

Mesoporous ball-milling iron-loaded biochar for enhanced sorption of reactive red: Performance and mechanisms.

Environ Pollut 2021 Aug 17;290:117992. Epub 2021 Aug 17.

School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.

In order to solve the low sorption capacity of pristine biochar for anionic pollutants, e.g., reactive red 120 (RR120), a novel mesoporous Fe-biochar composite was fabricated in this study by combination of Fe-loading and ball-milling methods. The ball-milling Fe-biochar composite could effectively remove RR120 by up to 90.1 mg g at pH of 7.5, and slightly alkaline condition was preferred. Adsorption kinetics showed that ball-milling Fe-biochar composite could quickly sorb RR120 with the rate constant (k) of 2.07 g mg min (pH = 7.5). Positive surface charge and large surface area were responsible for the outstanding removal performance of RR120 by ball-milling Fe-biochar composite: (1) The adscititious Fe would be converted to β-FeOOH during pyrolysis, which significantly improved the zeta potential of biochar and thus facilitated the electrostatic adsorption for RR120, which contributed to 42.3% and 85.5% at pH of 3 and 7.5, respectively; (2) Ball-milling effectively increased the specific surface area and uniformed the pore size distribution, which could provide more sorption sites and expedite the diffusion of RR120 molecules, shortening the time from several hours to less than 15 min. Findings of this study not only provide a feasible modification method for biochar to adsorb anionic pollutants efficiently and rapidly, but also help to reveal the roles of Fe-loading and ball-milling in enhancing adsorption capacity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2021.117992DOI Listing
August 2021

RNA-seq analysis of ischemia stroke and normal brain in a tree shrew model with or without type 2 diabetes mellitus.

Metab Brain Dis 2021 Oct 21;36(7):1889-1901. Epub 2021 Aug 21.

Department of Endocrinology, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, Yunnan, China.

Nowadays, similar strategies have been used for the treatment and prevention of acute stroke in both diabetes mellitus (DM) and non-DM populations. These strategies were analyzed to provide an experimental basis for the clinical prevention and treatment of stroke in patients both with and without DM. Tree shrews were randomly divided into control, DM, ischemic stroke (IS), and DMIS groups with 18 animals in each group. Serum biochemical indicators were used to assess metabolic status. Neural tissue damage was determined using triphenyl tetrazolium chloride staining, H-E staining, and electron microscopy. Differential gene expression of neural tissue between the DM and control groups and the IS and DMIS groups was measured using RNA-seq analysis. The serum glucose levels of the DM and DMIS groups were significantly higher than other groups. In the DMIS group, the infarct size was significantly larger than in the IS group (19.56 ± 1.25%), with a more obvious abnormal ultrastructure of neural cells. RNA-seq analysis showed that the expression of IL-8, C-C motif chemokine 2 (CCL2), and alpha-1-antichymotrypsin was significantly higher in the DM group than in the control group. The CCL7, ATP-binding cassette sub-family A member 12, and adhesion G protein-coupled receptor E2 levels were significantly higher in the DMIS group than in the IS group. For the prevention and treatment of stroke in patients with DM, reducing the inflammatory state of the nervous system may reduce the incidence of stroke and improve the prognosis of neurological function after IS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11011-021-00813-5DOI Listing
October 2021

Relationship between serum uric acid level and nonalcoholic fatty liver disease in type 2 diabetes patients.

Medicine (Baltimore) 2021 Aug;100(33):e26946

Department of Hepatology, Yantai Qishan Hospital, Yantai, China.

Abstract: This study aimed to investigate the association between serum uric acid (SUA) level and nonalcoholic fatty liver disease (NAFLD) in patients with type 2 diabetes (T2DM).T2DM patients hospitalized in the Department of Hepatology, Yantai Qishan Hospital, between April 2012 and December 2018 were classified into the NAFLD group and the non-NAFLD group. Clinical data, glucose and lipid metabolism biomarkers, and liver and kidney function parameters were retrospectively collected.Five hundred eighty-three T2DM patients met the inclusion and exclusion criteria; 227 patients were included in the non-NAFLD group and 356 patients were included in the NAFLD group. Multiple linear regression analyses showed that SUA was positively correlated with body mass index (P = .003), triglycerides (P = .009), aspartate aminotransferase (P = .036), and alanine aminotransferase (P = .038) and negatively correlated with estimated glomerular filtration rate (P < .001) in T2DM patients. Multivariate regression analyses demonstrated that after adjusting for confounding factors, the SUA tertile was still significantly associated with NAFLD occurrence in T2DM patients (P for trend = .008). With reference to SUA tertile I, the odds ratios for NAFLD in the SUA tertile II and tertile III groups were 1.729 (95% confidence interval [CI]: 1.086-2.753) and 2.315 (95% CI: 1.272-4.213), respectively.The level of SUA in T2DM patients was associated with the occurrence of NAFLD. Elevated SUA was associated with a significantly increased prevalence of NAFLD. The SUA level was an independent risk factor for NAFLD occurrence in patients with T2DM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000026946DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376353PMC
August 2021

Differential Effects of 17,18-EEQ and 19,20-EDP Combined with Soluble Epoxide Hydrolase Inhibitor -TUCB on Diet-Induced Obesity in Mice.

Int J Mol Sci 2021 Jul 31;22(15). Epub 2021 Jul 31.

Department of Nutrition, University of Tennessee, Knoxville, TN 37996, USA.

17,18-Epoxyeicosatetraenoic acid (17,18-EEQ) and 19,20-epoxydocosapentaenoic acid (19,20-EDP) are bioactive epoxides produced from n-3 polyunsaturated fatty acid eicosapentaenoic acid and docosahexaenoic acid, respectively. However, these epoxides are quickly metabolized into less active diols by soluble epoxide hydrolase (sEH). We have previously demonstrated that an sEH inhibitor, -TUCB, decreased serum triglycerides (TG) and increased lipid metabolic protein expression in the brown adipose tissue (BAT) of diet-induced obese mice. This study investigates the preventive effects of -TUCB (T) alone or combined with 19,20-EDP (T + EDP) or 17,18-EEQ (T + EEQ) on BAT activation in the development of diet-induced obesity and metabolic disorders via osmotic minipump delivery in mice. Both T + EDP and T + EEQ groups showed significant improvement in fasting glucose, serum triglycerides, and higher core body temperature, whereas heat production was only significantly increased in the T + EEQ group. Moreover, both the T + EDP and T + EEQ groups showed less lipid accumulation in the BAT. Although UCP1 expression was not changed, PGC1α expression was increased in all three treated groups. In contrast, the expression of CPT1A and CPT1B, which are responsible for the rate-limiting step for fatty acid oxidation, was only increased in the T + EDP and T + EEQ groups. Interestingly, as a fatty acid transporter, CD36 expression was only increased in the T + EEQ group. Furthermore, both the T + EDP and T + EEQ groups showed decreased inflammatory NFκB signaling in the BAT. Our results suggest that 17,18-EEQ or 19,20-EDP combined with -TUCB may prevent high-fat diet-induced metabolic disorders, in part through increased thermogenesis, upregulating lipid metabolic protein expression, and decreasing inflammation in the BAT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms22158267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347952PMC
July 2021

QTL mapping for starch paste viscosity of rice (Oryza sativa L.) using chromosome segment substitution lines derived from two sequenced cultivars with the same Wx allele.

BMC Genomics 2021 Aug 5;22(1):596. Epub 2021 Aug 5.

Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice R&D Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, 210014, China.

Background: The eating and cooking qualities (ECQs) of rice (Oryza sativa L.) are key characteristics affecting variety adoption and market value. Starch viscosity profiles tested by a rapid visco analyzer (RVA) offer a direct measure of ECQs and represent the changes in viscosity associated with starch gelatinization. RVA profiles of rice are controlled by a complex genetic system and are also affected by the environment. Although Waxy (Wx) is the major gene controlling amylose content (AC) and ECQs, there are still other unknown genetic factors that affect ECQs.

Results: Quantitative trait loci (QTLs) for starch paste viscosity in rice were analyzed using chromosome segment substitution lines (CSSLs) developed from the two cultivars 9311 and Nipponbare, which have same Wx-b allele. Thus, the effect of the major locus Wx was eliminated and the other locus associated with the RVA profile could be identified. QTLs for seven parameters of the starch RVA profile were tested over four years in Nanjing, China. A total of 310 QTLs were identified (from 1 to 55 QTLs per trait) and 136 QTLs were identified in more than one year. Among them, 6 QTLs were stalely detected in four years and 26 QTLs were detected in at least three years including 13 pleiotropic loci, controlling 2 to 6 RVA properties simultaneously. These stable QTL hotspots were co-located with several known starch synthesis-related genes (SSRGs). Sequence alignments showed that nucleotide and amino acid sequences of most SSRGs were different between the two parents. Finally, we detected stable QTLs associated with multiple starch viscosity traits near Wx itself, supporting the notion that additional QTLs near Wx control multiple characteristic values of starch viscosity.

Conclusions: By eliminating the contribution from the major locus Wx, multiple QTLs associated with the RVA profile of rice were identified, several of which were stably detected over four years. The complexity of the genetic basis of rice starch viscosity traits might be due to their pleiotropic effects and the multiple QTL hot spots. Minor QTLs controlling starch viscosity traits were identified by using the chromosome segment substitution strategy. Allele polymorphism might be the reason that QTLs controlling RVA profile characteristics were detected in some known SSRG regions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12864-021-07913-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8340499PMC
August 2021

Enantio- and Diastereodivergent Construction of 1,3-Nonadjacent Stereocenters Bearing Axial and Central Chirality through Synergistic Pd/Cu Catalysis.

J Am Chem Soc 2021 Aug 5;143(32):12622-12632. Epub 2021 Aug 5.

Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs, Frontiers Science Center for Transformative Molecules, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.

In contrast to the widely explored methods for the asymmetric synthesis of molecules bearing a single stereocenter or adjacent stereocenters, the concurrent construction of 1,3-stereogenic centers in an enantio- and diastereoselective manner remains a challenge, especially in acyclic systems. Herein, we report an enantio- and diastereodivergent construction of 1,3-nonadjacent stereocenters bearing allenyl axial and central chirality through synergistic Pd/Cu-catalyzed dynamic kinetic asymmetric allenylation with racemic allenylic esters. The protocol is suitable for a wide range of substrates including the challenging allenylic esters with less sterically bulky substituents and provided chiral allenylic products bearing 1,3-nonadjacent stereocenters with high levels of enantio- and diastereoselectivities (up to >20:1 dr and >99% ee). Furthermore, several representative transformations involving axial-to-central chirality transfer were conducted, affording useful structural motifs containing nonadjacent stereocenters in a diastereodivergent manner.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.1c05087DOI Listing
August 2021

RNA Demethylase FTO Mediated RNA mA Modification Is Involved in Maintaining Maternal-Fetal Interface in Spontaneous Abortion.

Front Cell Dev Biol 2021 19;9:617172. Epub 2021 Jul 19.

Department of Gynecology, The Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

The N6-methyladenosine (mA) RNA modification regulates the expression of genes associated with various biological and pathological processes, including spontaneous abortion (SA). The aim of this study was to determine the role of the mA demethylase fat mass and obesity (FTO)- associated protein in SA. The , and mRNA levels were significantly lower in the chorionic villi obtained from spontaneously aborted pregnancies compared to that of normal pregnancies, while the expression levels of and were significantly elevated. However, , , and were elevated with no statistical significance between groups. In addition, MDA was elevated and SOD levels were decreased in the villi tissues of the SA group compared to the normal group, which was indicative of placental oxidative stress in the former. Furthermore, the expression of FTO and HLA-G were significantly decreased in the trophoblasts of the SA patients compared to that of normal pregnant women, while that of mA was markedly higher in the former. In addition, the and mRNA levels were downregulated in the SA versus the control group, and that of , , and were upregulated. Finally, The RIP assay showed significantly decreased levels of FTO-bound , and RNA in SA patients ( < 0.05), which corresponded to an increase in transcripts enriched with the mA antibody ( < 0.05). However, compared with normal pregnant women, the levels of , , , and mRNA bound by YTHDF2 were significantly decreased in SA patients. Compared to the normal pregnant women, both FTO- and mA-bound were significantly increased in SA patients ( < 0.05), but YTHDF2 almost unbound to mRNA. In summary, the downregulation of FTO in the chorionic villi disrupts immune tolerance and angiogenesis at the maternal-fetal interface, resulting in aberrant methylation and oxidative stress that eventually leads to SA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2021.617172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326377PMC
July 2021

Virus-like vesicles based on SFV-containing rabies virus glycoprotein make a safe and efficacious rabies vaccine candidate in a mouse model.

J Virol 2021 Aug 4:JVI0079021. Epub 2021 Aug 4.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.

Rabies is a fatal zoonosis causing encephalitis in mammals, and vaccination is the most effective method to control and eliminate rabies. Virus-like vesicles (VLVs), which are characterized as infectious, self-propagating membrane-enveloped particles composed of only Semliki Forest virus (SFV) replicase and vesicular stomatitis virus glycoprotein (VSV-G), have been proven safe and efficient as vaccine candidates. However, previous studies showed that VLVs containing rabies virus glycoprotein (RABV-G) grew at relatively low titers in cells, impeding their potential use as a rabies vaccine. In this study, we constructed novel VLVs by transfection of a mutant SFV RNA replicon encoding RABV-G. We found these VLVs could self-propagate efficiently in cell culture and could evolve to high titers (approximately 10 FFU/ml) by extensive passaging 25 times in BHK-21 cells. Furthermore, we found that the evolved amino acid change in SFV nsP1 at positions 470 and 482 was critical for this high-titer phenotype. Remarkably, VLVs could induce robust type I IFN expression in BV2 cells and were highly sensitive to IFN-α. We found that direct inoculation of VLVs into the mouse brain caused lesser body weight loss, mortality and neuroinflammation compared with RABV vaccine strain. Finally, it could induce increased generation of germinal centre (GC) B cells, plasma cells (PCs) and virus-neutralizing antibodies (VNAs), as well as provide protection against virulent RABV challenge in immunized mice. This study demonstrated that VLVs containing RABV-G could proliferate in cells and were highly evolvable, revealing the feasibility of developing an economic, safe and efficacious rabies vaccine. VLVs have been shown to represent a more versatile and superior vaccine platform. In previous studies, VLVs containing the Semliki Forest Virus replicase (SFV nsP1-4) and rabies virus glycoprotein (RABV-G) grew to relatively low titers in cells. In our study, we not only succeeded in generating VLVs that proliferate in cells and stably express RABV-G, the VLVs that evolved grew to higher titers reaching 10 FFU/ml. We also found that nucleic acid changes at positions 470 and 482 in nsP1 were vital for this high-titer phenotype. Moreover, the VLVs that evolved in our studies were highly attenuated in mice, induced potent immunity and protected mice from lethal RABV infection. Collectively, our study showed that high titers of VLVs containing RABV-G were achieved demonstrating that these VLVs could be an economical, safe, and efficacious rabies vaccine candidate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1128/JVI.00790-21DOI Listing
August 2021

The Pathogenic Features of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): Possible Mechanisms for Immune Evasion?

Front Immunol 2021 14;12:693579. Epub 2021 Jul 14.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.

Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is a newly emerging, highly transmitted and pathogenic coronavirus that has caused global public health events and economic crises. As of March 4, 2021, more than 100 million people have been infected, more than 2 million deaths have been reported worldwide, and the numbers are continuing to rise. To date, a specific drug for this lethal virus has not been developed to date, and very little is currently known about the immune evasion mechanisms of SARS-CoV-2. The aim of this review was to summarize and sort dozens of published studies on PubMed to explore the pathogenic features of SARS-CoV-2, as well as the possible immune escape mechanisms of this virus.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.693579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317057PMC
August 2021

Effectiveness and safety of iguratimod treatment in patients with active rheumatoid arthritis in Chinese: A nationwide, prospective real-world study.

Lancet Reg Health West Pac 2021 May 22;10:100128. Epub 2021 Mar 22.

Department of Rheumatology and Immunology, Peking University People's Hospital, No. 11 Xizhimen South Street, Beijing, China.

Background: There is heterogeneity in the clinical manifestations and responses to drugs in RA patients due to variety of factors such as genes and environment. Despite advances in the treatment of rheumatoid arthritis (RA), approximately 40% of RA patients still do not achieve primary clinical outcomes in randomized trials, and its low remission rate and high economic consumption remain unresolved, especially in developing countries. Iguratimod (IGU) is a new disease-modifying anti-rheumatic drug (DMARD) with a low price that has demonstrated good efficacy and safety in clinical trials and was approved for active RA in China and Japan. As the most populous country in the Western Pacific region, it is warranted to conduct a study with a large scale of patients in a real-life setting. Our study confirms the new option for RA patients, which is potentially benificial for public health in developing countries.

Methods: This was a nationwide, prospective real-world study of IGU. Eligible subjects were active adult RA patients who aged 18 to 85 with or without multiple comorbidities such as hypertension and diabetes with DMARDs at a stable dosage for at least 12 weeks, or without ongoing DMARDs. A two-stage design was used for this study. In the first stage (the first 12 weeks), IGU 25 mg bid was added as monotherapy or to the background therapy, and in the second stage (the latter 12 weeks), adjustment of RA medicines other than IGU was allowed according to the participants' disease activity. The primary endpoints were American College of Rheumatology 20% response (ACR20) 24 weeks and adverse events during 24 weeks. The secondary endpoints were ACR50 and ACR70 over 24 weeks, the changes of DAS28 and Health Assessment Questionnaire (HAQ) at week 12 and week 24 from baseline. The trial was registered with ClinicalTrials.gov, number NCT01554917.

Findings: Between March 2012 and January 2015, 1759 participants were enrolled, of whom 81•5% (1433/1759) completed the study. Notably, 1597 patients in the full analysis set were assessed for the effectiveness and 1751 patients were in the safety analysis set; 71•9% (1148/1597) of the patients achieved the primary endpoint of ACR20 response at week 24, and 51•7% (906/1751) patients had at least 1 adverse event (AE). The incidence of the clinical significant AE (grade≥3) of special interest was 3•4% (54 patients for grade 3 and 6 patients for grade 4), and 0•7% (13/1751) of patients developed SAEs associated with IGU. The most common clinical significant AEs were infection in 0•6% (10/1751) of the patients, abdominal discomfort in 0•5% (9/1751) of the patients including 0•2% (3/1751) gastric ulcer, fracture in 0•4% (7/1751), and increased alanine aminotransferase (ALT) in 0•2% (3/1751) of the patients. The secondary endpoint of ACR50 and ACR70 response rates at week 24 were 47•4% (757/1597) and 24•0% (384/1597). DAS28 was 4•11±1•27 and 3•75±1•32 at week 12 and 24, which was significantly decreased -1•40±1•10 and -1•75±1•26 compared with baseline (<0•001) respectively. Changes in HAQ at week 12 and 24 from baseline were -7•4 ± 9•18 and -8•5 ± 9•97, respectively (all <0•001). Stratified analysis results showed that the patients with shorter disease duration, male gender had better response to IGU. There was no significant difference in ACR20/50/70 responses between elderly patients(≥65 years) and younger patients(<65 years), IGU monotherapy or combined with other DMARDs. However, more fractures (1•1% 0•5%;  = 0•64) and infections (8•7% 7•9%;  = 0•69) were observed in elderly patients in our study.

Interpretation: Our results confirmed the effectiveness and safety of IGU as a new DMARD for active patients with RA as monotherapy or combination therapy.

Funding: This study was supported by "the 11th Five-Year-Plan for Science and Technology Support Program (2012ZX09104-103-01)".
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lanwpc.2021.100128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315426PMC
May 2021

Copper exposure induces hepatic G0/G1 cell-cycle arrest through suppressing the Ras/PI3K/Akt signaling pathway in mice.

Ecotoxicol Environ Saf 2021 Oct 14;222:112518. Epub 2021 Jul 14.

College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China.

Copper (Cu), as a common chemical contaminant in environment, is known to be toxic at high concentrations. The current research demonstrates the effects of copper upon hepatocyte cell-cycle progression (CCP) in mice. Institute of cancer research (ICR) mice (n = 240) at an age of four weeks were divided randomly into groups treated with different doses of Cu (0, 4, 8, and 16 mg/kg) for 21 and 42 days. Results showed that high Cu exposure caused hepatocellular G0/G1 cell-cycle arrest (CCA) and reduced cell proportion in the G2/M phase. G0/G1 CCA occurred with down-regulation (p < 0.05) of Ras, p-PI3K (Tyr458), p-Akt (Thr308), p-forkhead box O3 (FOXO3A) (Ser253), p-glycogen synthase kinase 3-β (GSK3-β) (Ser9), murine double minute 2 (MDM2) protein, and mRNA expression levels, and up-regulation (p < 0.05) of PTEN, p-p53 (Ser15), p27, p21 protein, and mRNA expression levels, which subsequently suppressed (p < 0.05) the protein and mRNA expression levels of CDK2/4 and cyclin E/D. These results indicate that Cu exposure suppresses the Ras/PI3K/Akt signaling pathway to reduce the level of CDK2/4 and cyclin E/D, which are essential for the G1-S transition, and finally causes hepatocytes G0/G1 CCA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2021.112518DOI Listing
October 2021

Chemical and photo-initiated aging enhances transport risk of microplastics in saturated soils: Key factors, mechanisms, and modeling.

Water Res 2021 Sep 6;202:117407. Epub 2021 Jul 6.

School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Engineering Research Center for Solid Waste Treatment and Resource Recovery, Shanghai 200092, China. Electronic address:

Microplastics (MPs) inevitably undergo aging transformation and transport process in environmental compartments. In this study, the polystyrene MPs were aged via three different oxidation methods including persulfate oxidation (PS), UV irradiation (UV), and UV irradiated persulfate oxidation (UVPS). All three treatments induced the great transformation of MPs, with the significant increase in surface roughness and in oxygen-containing functional groups, i.e., COOH or COOC. The UVPS aging showed synergetic effect due to the strengthened photo-initiated chemical oxidation, compared to UV and PS alone. All aged MPs exhibited the enhanced transport (34.9%-89.2%) in sandy and clay loam soils than pristine MPs (30.5%), and the synergetic effect was also observed in the transport behaviors of the UVPS MPs. Higher transport of MPs and aged MPs occurred in sandy soil than that in clay loam soil since the latter one contained high Fe minerals that tend to retain MPs, which was confirmed by the model quartz sand column experiment. Modeling on the migration of MPs retained in soil under a rainstorm scenario showed that the aged MPs had the stronger remobility and greater proportion of cumulative flux than pristine ones in the soil profile. These findings provided new insights on the fate and transport of MPs in natural soil and their potential risk to groundwater contamination.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.watres.2021.117407DOI Listing
September 2021

Murine Ifit3 restricts the replication of Rabies virus both and .

J Gen Virol 2021 07;102(7)

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China.

Rabies virus (RABV) infection can initiate the host immune defence response and induce an antiviral state characterized by the expression of interferon (IFN)-stimulated genes (ISGs), among which the family of genes of IFN-induced protein with tetratricopeptide repeats (Ifits) are prominent representatives. Herein, we demonstrated that the mRNA and protein levels of Ifit1, Ifit2 and Ifit3 were highly increased in cultured cells and mouse brains after RABV infection. Recombinant RABV expressing Ifit3, designated rRABV-Ifit3, displayed a lower pathogenicity than the parent RABV in C57BL/6 mice after intramuscular administration, and Ifit3-deficient mice exhibited higher susceptibility to RABV infection and higher mortality during RABV infection. Moreover, compared with their individual expressions, co-expression of Ifit2 and Ifit3 could more effectively inhibit RABV replication . These results indicate that murine Ifit3 plays an essential role in restricting the replication and reducing the pathogenicity of RABV. Ifit3 acts synergistically with Ifit2 to inhibit RABV replication, providing further insight into the function and complexity of the Ifit family.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1099/jgv.0.001619DOI Listing
July 2021

Carboxypeptidase E Regulates Activity-Dependent TrkB Neuronal Surface Insertion and Hippocampal Memory.

J Neurosci 2021 Aug 15;41(33):6987-7002. Epub 2021 Jul 15.

Departments of Anatomy and Neurobiology and

Activity-dependent insertion of the tropomyosin-related kinase B (TrkB) receptor into the plasma membrane can explain, in part, the preferential effect of brain-derived neurotrophic factor (BDNF) on active neurons and synapses; however, the underlying molecular mechanisms remain obscure. Here, we report a novel function for carboxypeptidase E (CPE) in controlling chemical long-term potentiation stimuli-induced TrkB surface delivery in hippocampal neurons. Total internal reflection fluorescence assays and line plot assays showed that CPE facilitates TrkB transport from dendritic shafts to the plasma membrane. The Box2 domain in the juxtamembrane region of TrkB and the C terminus of CPE are critical for the activity-dependent plasma membrane insertion of TrkB. Moreover, the transactivator of transcription TAT-CPE, which could block the association between CPE and TrkB, significantly inhibited neuronal activity-enhanced BDNF signaling and dendritic spine morphologic plasticity in cultured hippocampal neurons. Microinfusion of TAT-CPE into the dorsal hippocampus of male C57BL/6 mice inhibited the endogenous interaction between TrkB and CPE and diminished fear-conditioning-induced TrkB phosphorylation, which might lead to an impairment in hippocampal memory acquisition and consolidation but not retrieval. These results suggest that CPE modulates activity-induced TrkB surface insertion and hippocampal-dependent memory and sheds light on our understanding of the role of CPE in TrkB-dependent synaptic plasticity and memory modulation. It is well known that BDNF acts preferentially on active neurons; however, the underlying molecular mechanism is not fully understood. In this study, we found that the cytoplasmic tail of CPE could interact with TrkB and facilitate the neuronal activity-dependent movement of TrkB vesicles to the plasma membrane. Blocking the association between CPE and TrkB decreased fear-conditioning-induced TrkB phosphorylation and led to hippocampal memory deficits. These findings provide novel insights into the role of CPE in TrkB intracellular trafficking as well as in mediating BDNF/TrkB function in synaptic plasticity and hippocampal memory.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1523/JNEUROSCI.0236-21.2021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8372023PMC
August 2021

Comparison of 10.6 μm Laser Moxibustion with Traditional Moxibustion in Knee Osteoarthritic Therapy: A Randomized Noninferiority Clinical Trial.

Photobiomodul Photomed Laser Surg 2021 Jul;39(7):492-498

Acupuncture and Tuina School, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

To investigate the noninferiority of 10.6 μm laser moxibustion (LM) to traditional moxibustion (TM) in knee osteoarthritis (KOA). Ninety-two patients were recruited and randomly placed into one of two groups: 10.6 μm LM or TM in a 1:1 ratio. Each patient received 12 sessions of LM or TM, focusing on the ST-35 and Ashi acupoint. The sessions took place over 4 weeks, three times a week, and were followed up over 8 weeks. The endpoint outcomes were separated into two categories, primary and secondary. The primary endpoint was assessed at the end of the 4-week treatment, using the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) pain score. The secondary endpoint was evaluated at the end of the trial and consisted of the WOMAC function and stiffness score, visual analog pain [visual analog scale (VAS)] score, and 15-m walking time test. In addition, safety evaluation was performed throughout the trial. Among the 92 randomized participants, 86 (93.48%) completed the trial; 43 in each group. The WOMAC pain score improved dramatically between the LM and TM groups, with a mean difference of 20.61 [95% confidence interval (CI): -2.28 to 43.50]. Given that the lower boundary of 95% CI was greater than -18.49, noninferiority was established. In addition, both LM and TM significantly decreased the WOMAC (pain, function and stiffness) score, VAS score ( < 0.05), and the 15-m walking time at the end of the trial. Interestingly, there were not significant differences between LM and TM ( > 0.05), suggesting that both are equally effective in treating KOA. Finally, among the 92 patients, 17 (18.48%) adverse effects were documented, namely 5 (10.87%) in the LM-treated group and 11 (26.09%) in the TM-treated group. 10.6 μm LM is not inferior to TM in treating KOA. Moreover, both LM and TM dramatically alleviated knee pain and enhanced function of knees. Clinical Trial Registration number: ISRCTN registry trial identifier: 14604492.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/photob.2021.0030DOI Listing
July 2021
-->