Publications by authors named "Ling Zhang"

3,081 Publications

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Long-Term Remission After Matched Sibling Donor Hematopoietic Cell Transplantation in a Patient With Primary Cutaneous CD8+ Aggressive Epidermotropic Cytotoxic T-Cell Lymphoma.

Cureus 2021 May 20;13(5):e15132. Epub 2021 May 20.

Hematology and Oncology, Moffitt Cancer Center, Tampa, USA.

Primary cutaneous CD8+ aggressive epidermotropic cytotoxic T-cell lymphoma (CD8+ PCAECTL) is an extremely rare neoplasm with a poor prognosis. Chemotherapy typically does not result in a sustained response, and hematopoietic stem cell transplant (HSCT) is the only therapy that has been shown to produce a durable response of any kind. Here, we report a case of a 25-year-old previously healthy male who presented with a painful ulcerative lesion on the bottom of his right great toe and local lymphadenopathy. The biopsy of the lesion was consistent with CD8+ PCAECTL. He received immediate chemotherapy followed by matched related donor HSCT (MRD-HSCT) and remained in complete remission (CR) for eight years post-transplant, longer than any CR reported in the literature. In conclusion, our report provides clinical evidence that early transplant consult and donor search is one of the key factors in the management of CD8+ PCAECTL.
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http://dx.doi.org/10.7759/cureus.15132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214246PMC
May 2021

Cordycepin inhibits inflammatory responses through suppression of ERK activation in zebrafish.

Dev Comp Immunol 2021 Jun 19:104178. Epub 2021 Jun 19.

College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China. Electronic address:

As a natural extract, cordycepin has been shown to play important regulatory roles in many life activities. In the study, the effects of cordycepin on inflammatory responses and the underlying mechanisms was explored using a zebrafish model. In the model of LPS-induced inflammation, cordycepin was found to significantly inhibited the expression of pro-inflammatory cytokines such as tnf-α, il-1β, il-6, and il-8. Using in vivo imaging model, cordycepin significantly inhibited fluorescent-labeled neutrophils migrating towards injury sites. Furthermore, results showed that the phosphorylation level of ERK protein dramatically decreased after cordycepin treatment. Meanwhile, the ERK inhibitor, PD0325901, significantly inhibited the expression of pro-inflammatory cytokines in LPS-induced inflammatory model and neutrophils migration in the caudal fin injury model. This study indicated the important roles of cordycepin in inhibiting LPS and injury-induced inflammation and preliminarily explained the role of ERK protein in this process.
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http://dx.doi.org/10.1016/j.dci.2021.104178DOI Listing
June 2021

Environmental and Genetic Determinants of Major Chronic Disease in Beijing-Tianjin-Hebei Region: Protocol for a Community-Based Cohort Study.

Front Public Health 2021 4;9:659701. Epub 2021 Jun 4.

Department of Epidemiology and Health Statistics, School of Public Health, Capital Medical University, Beijing, China.

Personal lifestyle and air pollution are potential risk factors for major non-communicable diseases (NCDs). However, these risk factors have experienced dramatic changes in the Beijing-Tianjin-Hebei (BTH) region in recent years, and few cohorts have focused on identifying risk factors for major NCDs in this specific region. The current study is a large, prospective, long-term, population-based cohort study that investigated environmental and genetic determinants of NCDs in BTH areas. The results of this study may provide scientific support for efforts to develop health recommendations for personalized prevention. About 36,000 participants 18 years or older would be obtained by multistage, stratified cluster sampling from five cities for the baseline assessment. Participants underwent seven examinations primarily targeting respiratory and circulatory system function and filled out questionnaires regarding lifestyle behavior, pollutant exposure, medical and family history, medication history, and psychological factors. Biochemistry indicators and inflammation markers were tested, and a biobank was established. Participants will be followed up every 2 years. Genetic determinants of NCDs will be demonstrated by using multiomics, and risk prediction models will be constructed using machine learning methods based on a multitude of environmental exposure, examination data, biomarkers, and psychosocial and behavioral assessments. Significant spatial and temporal differentiation is well-suited to demonstrating the health determinants of NCDs in the BTH region, which may facilitate public health strategies with respect to disease prevention and survivorship-related aspects.
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http://dx.doi.org/10.3389/fpubh.2021.659701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212971PMC
June 2021

Boosting High-Rate Zinc-Storage Performance by the Rational Design of MnO Nanoporous Architecture Cathode.

Nanomicro Lett 2019 Dec 31;12(1):14. Epub 2019 Dec 31.

Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.

Manganese oxides are regarded as one of the most promising cathode materials in rechargeable aqueous Zn-ion batteries (ZIBs) because of the low price and high security. However, the practical application of MnO in ZIBs is still plagued by the low specific capacity and poor rate capability. Herein, highly crystalline MnO materials with interconnected mesostructures and controllable pore sizes are obtained via a ligand-assisted self-assembly process and used as high-performance electrode materials for reversible aqueous ZIBs. The coordination degree between Mn and citric acid ligand plays a crucial role in the formation of the mesostructure, and the pore sizes can be easily tuned from 3.2 to 7.3 nm. Ascribed to the unique feature of nanoporous architectures, excellent zinc-storage performance can be achieved in ZIBs during charge/discharge processes. The MnO electrode exhibits high reversible capacity (233 mAh g at 0.3 A g), superior rate capability (162 mAh g retains at 3.08 A g) and remarkable cycling durability over 3000 cycles at a high current rate of 3.08 A g. Moreover, the corresponding electrode reaction mechanism is studied in depth according to a series of analytical methods. These results suggest that rational design of the nanoporous architecture for electrode materials can effectively improve the battery performance.
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http://dx.doi.org/10.1007/s40820-019-0351-4DOI Listing
December 2019

Effectiveness of sharp recanalization of superior vena cava-right atrium junction occlusion.

World J Clin Cases 2021 Jun;9(16):3848-3857

Department of Interventional Radiology, The Affiliated Hospital of Guizhou Medical University, Guiyang 550000, Guizhou Province, China.

Background: Conventional recanalization techniques may fail in patients with completely occluded superior vena cava (SVC).

Aim: To analyze the effectiveness and complications of sharp recanalization for completely occluded SVC.

Methods: This was a retrospective study of patients that underwent puncture and recanalization of the SVC between January 2016 and December 2017 at our hospital. Sharp recanalization was performed using the RUPS-100 system. The patients were followed for 12 mo. The main outcomes were the patency rate of SVC and arteriovenous fistula flow during dialysis.

Results: The procedure was successful in all 14 patients (100%). Blood pressure in the distal SVC decreased in all 14 cases (100%) from 26.4 ± 2.7 cmHO to 14.7 ± 1.3 cmHO ( < 0.05). The first patency rates of the SVC at 24 h and at 3, 6, 9 and 12 mo after sharp recanalization were 100%, 92.9%, 85.7%, 78.6% and 71.4%, respectively. There were two (14.3%) severe, one (7.1%) moderate and one (7.1%) minor complication. The severe complications included one case of pericardial tamponade and one case of hemothorax.

Conclusion: The results suggest that sharp recanalization can be an additional tool to extend or renew the use of an occluded upper extremity access for hemodialysis. This could be of use in patients with long-term maintenance hemodialysis in whom the maintenance of central venous access is often a challenge.
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http://dx.doi.org/10.12998/wjcc.v9.i16.3848DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180228PMC
June 2021

Cryogenic Exfoliation of 2D Stanene Nanosheets for Cancer Theranostics.

Nanomicro Lett 2021 Mar 10;13(1):90. Epub 2021 Mar 10.

Center for Nanomedicine and Department of Anesthesiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA.

Stanene (Sn)-based materials have been extensively applied in industrial production and daily life, but their potential biomedical application remains largely unexplored, which is due to the absence of the appropriate and effective methods for fabricating Sn-based biomaterials. Herein, we explored a new approach combining cryogenic exfoliation and liquid-phase exfoliation to successfully manufacture two-dimensional (2D) Sn nanosheets (SnNSs). The obtained SnNSs exhibited a typical sheet-like structure with an average size of ~ 100 nm and a thickness of ~ 5.1 nm. After PEGylation, the resulting PEGylated SnNSs ([email protected]) exhibited good stability, superior biocompatibility, and excellent photothermal performance, which could serve as robust photothermal agents for multi-modal imaging (fluorescence/photoacoustic/photothermal imaging)-guided photothermal elimination of cancer. Furthermore, we also used first-principles density functional theory calculations to investigate the photothermal mechanism of SnNSs, revealing that the free electrons in upper and lower layers of SnNSs contribute to the conversion of the photo to thermal. This work not only introduces a new approach to fabricate 2D SnNSs but also establishes the SnNSs-based nanomedicines for photonic cancer theranostics. This new type of SnNSs with great potential in the field of nanomedicines may spur a wave of developing Sn-based biological materials to benefit biomedical applications.
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http://dx.doi.org/10.1007/s40820-021-00619-1DOI Listing
March 2021

Integrin αβ Targeting DGEA-Modified Liposomal Doxorubicin Enhances Antitumor Efficacy against Breast Cancer.

Mol Pharm 2021 Jun 16. Epub 2021 Jun 16.

Key Laboratory of Drug Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, P.R. China.

Breast cancer was the leading cause of newly diagnosed cases of tumors in 2020, ranking as the second highest cause of female death. Chemotherapy remains the conventional treatment of choice for breast tumors in most clinical cases. However, it is often accompanied by a poor prognosis and severe side effects, resulting from an insufficient accumulation of the drug at tumor sites and an unsystematic distribution of the drug across the body. Inspired by the fact that breast tumor cells overexpress integrin αβ on the surface, we designed and constructed an integrin αβ targeting DGEA-modified liposomal doxorubicin (DGEA-Lipo-DOX) platform for application in breast cancer therapy. The DGEA-Lipo-DOX was stable with a uniform particle size of 121.1 ± 3.8 nm and satisfactory drug encapsulation. Demonstrated and , the constructed platform exhibited improved antitumor ability. The DGEA-Lipo-DOX showed 4-fold enhanced blood circulation and 6-fold increased accumulation of DOX at the tumor sites compared to those of free DOX, resulting in a significantly enhanced antitumor efficacy in tumor-bearing mice. A preliminary safety evaluation suggested that the systemic toxicity of DOX was relieved by DGEA-Lipo delivery. Collectively, binding integrin αβ by DGEA may represent an alternative therapeutic strategy for potentially safer breast cancer treatment.
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http://dx.doi.org/10.1021/acs.molpharmaceut.1c00132DOI Listing
June 2021

Ultrasensitive detection of SARS-CoV-2 spike protein in untreated saliva using SERS-based biosensor.

Biosens Bioelectron 2021 Jun 9;190:113421. Epub 2021 Jun 9.

State Key Laboratory on Integrated Optoelectronics, Jilin Key Laboratory on Advanced Gas Sensor, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, China. Electronic address:

Early diagnosis and monitoring of SARS-CoV-2 virus is essential to control COVID-19 outbreak. In this study, we propose a promising surface enhanced Raman scattering (SERS)-based COVID-19 biosensor for ultrasensitive detection of SARS-CoV-2 virus in untreated saliva. The SERS-immune substrate was fabricated by a novel oil/water/oil (O/W/O) three-phase liquid-liquid interfaces self-assembly method, forming two layers of dense and uniform gold nanoparticle films to ensure the reproducibility and sensitivity of SERS immunoassay. The detection was performed by an immunoreaction between the SARS-CoV-2 spike antibody modified SERS-immune substrate, spike antigen protein and Raman reporter-labeled immuno-Ag nanoparticles. This SERS-based biosensor was able to detect the SARS-CoV-2 spike protein at concentrations of 0.77 fg mL in phosphate-buffered saline and 6.07 fg mL in untreated saliva. The designed SERS-based biosensor exhibited excellent specificity and sensitivity for SARS-CoV-2 virus without any sample pretreatment, providing a potential choice for the early diagnosis of COVID-19.
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http://dx.doi.org/10.1016/j.bios.2021.113421DOI Listing
June 2021

Reproductive toxicity of cadmium in pubertal male rats induced by cell apoptosis.

Toxicol Ind Health 2021 Jun 15:7482337211022615. Epub 2021 Jun 15.

School of Public Health, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, 481115Wuhan University of Science and Technology, Wuhan, China.

Cadmium (Cd) is a heavy metal that is widely present in modern industrial production. It is a known, highly toxic environmental endocrine disruptor. Long-term exposure to Cd can cause varying degrees of damage to the liver, kidney, and reproductive system of organisms, especially the male reproductive system. This study aimed to explore the mechanism of Cd toxicity in the male reproductive system during puberty. Eighteen healthy 6-week-old male Sprague-Dawley rats were randomly divided into three groups (control group, low-dose group, and high-dose group) according to their body weight, with six in each group. Cd (0, 1, and 3 mg/kg/day) was given by gavage for 28 consecutive days. The results showed that Cd exposure to each dose group caused a decrease in the testicular organ coefficient and sperm count, compared with the control group. Cd exposure resulted in significant changes in testicular morphology in the 3 mg/kg/day Cd group. In the 1 and 3 mg/kg/day Cd groups, serum testosterone decreased and apoptosis of testicular cells increased significantly ( < 0.05). In addition, compared with the control group, the activity of glutathione peroxidase and superoxide dismutase in each Cd exposure dose group decreased, but the content of malondialdehyde in the high-dose, 3 mg/kg/day Cd treatment group significantly increased ( < 0.05). Although Cd exposure caused an increase in the messenger RNA (mRNA) levels of , and in the testicular tissues ( < 0.05), expression was unchanged ( > 0.05). The expression level of mRNA in testicular tissue of rats in the high-dose 3 mg/kg/day Cd group was increased ( < 0.05). Our data suggest that Cd affected testosterone levels, and apoptosis was observed in spermatids.
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http://dx.doi.org/10.1177/07482337211022615DOI Listing
June 2021

Dl-3-n-Butylphthalide Alleviates Behavioral and Cognitive Symptoms Via Modulating Mitochondrial Dynamics in the A53T-α-Synuclein Mouse Model of Parkinson's Disease.

Front Neurosci 2021 28;15:647266. Epub 2021 May 28.

Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Beijing, China.

Background: Aggregation and neurotoxicity of the presynaptic protein α-synuclein and the progressive loss of nigral dopaminergic neurons are believed to be the key hallmarks of Parkinson's disease (PD). A53T mutant α-synuclein causes early onset PD and more severe manifestations. A growing body of evidence shows that misfolding or deposition of α-synuclein is linked to the maintenance of mitochondrial dynamics, which has been proven to play an important role in the pathogenesis of PD. It has been observed that Dl-3-n-butylphthalide (NBP) may be safe and effective in improving the non-tremor-dominant PD. However, the potential mechanism remains unclear. This study aimed to investigate whether NBP could decrease the loss of dopaminergic neurons and α-synuclein deposition and explore its possible neuroprotective mechanisms.

Methods: A total of 20 twelve-month-old human A53T α-synuclein transgenic mice and 10 matched adult C57BL/6 mice were included in the study; 10 adult C57BL/6 mice were selected as the control group and administered with saline (0.2 ml daily for 14 days); 20 human A53T α-synuclein transgenic mice were randomly divided into A53T group (treated in the same manner as in the control group) and A53T + NBP group (treated with NBP 0.2 ml daily for 14 days). Several markers of mitochondrial fission and fusion and mitophagy were determined, and the behavioral, olfactory, and cognitive symptoms were assessed as well.

Results: In the present study, it was observed that the A53T-α-synuclein PD mice exhibited anxiety-like behavioral disturbance, impairment of coordination ability, memory deficits, and olfactory dysfunction, loss of dopaminergic neurons, and α-synuclein accumulation. Meanwhile, the mitofusin 1 expression was significantly decreased, and the mitochondrial number and dynamin-related protein 1, Parkin, and LC3 levels were increased. The detected levels of all markers were reversed by NBP treatment, and the mitochondrial morphology was partially recovered.

Conclusion: In the present study, a valuable neuropharmacological role of NBP has been established in the A53T-α-synuclein PD mouse model. Possible neuroprotective mechanisms might be that NBP is involved in the maintenance of mitochondrial dynamics including mitochondrial fission and fusion and clearance of damaged mitochondria. It is essential to perform further experiments to shed light on the precise mechanisms of NBP on mitochondrial homeostasis.
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http://dx.doi.org/10.3389/fnins.2021.647266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193045PMC
May 2021

Development of photocontrolled BRD4 PROTACs for tongue squamous cell carcinoma (TSCC).

Eur J Med Chem 2021 Jun 5;222:113608. Epub 2021 Jun 5.

Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China. Electronic address:

The catalytic properties of small-molecule proteolysis targeting chimeras (PROTACs) may lead to uncontrolled degradation. Therefore, the main disadvantages of PROTACs are non-cancer specificity and relatively high toxicity, which limit the clinical application of PROTACs. The photocontrolled PROTACs (photoPROTACs) were proposed to overcome this issue, in which they can be triggered by ultraviolet A (UVA) or visible light to induce the degradation of the target protein. Herein, we designed several photoPROTACs to cause the degradation of bromodomain-containing protein 4 (BRD4) on-demand using 365 nm light. The representative compound N2 is proved to induce the degradation of BRD4 upon irradiation. Moreover, compound N2 was successfully applied in vivo to inhibit tumor growth in a zebrafish xenograft model of skin cancer tongue squamous cell carcinoma (TSCC) in a photocontrol manner.
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http://dx.doi.org/10.1016/j.ejmech.2021.113608DOI Listing
June 2021

Molecular characterization, expression and functional analysis of yak IFITM3 gene.

Int J Biol Macromol 2021 Jun 11;184:349-357. Epub 2021 Jun 11.

Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610041, China.

IFITM3 is interferon-induced transmembrane 3, which plays an extremely key role in anti-proliferation, anti-virus and anti-tumor diseases. In this study, the yak (Bos grunniens) IFITM3 (BgIFITM3) gene contained a 5'-untranslated region (UTR) (25 bp), a coding region (441 bp), and a 3'-UTR (115 bp). The expression of BgIFITM3 gene in liver was significantly higher than that in heart, spleen, lung and kidney (P < 0.01). BgIFITM3 protein was localized on the yak hepatocyte plasma membrane, and its expression was significantly different between 1 day and 15 months of age (P < 0.05). Moreover, the prokaryotic expression vector of BgIFITM3 protein was constructed and expressed successfully, with a molecular weight of 19.5 kDa. The activities of yak hepatocyte were significantly inhibited after treating with BgIFITM3 protein (10 and 20 μg/mL) (P < 0.01). The expression levels of ERBB-2, IRS-1, PI3KR-1, AKT-1 and MAPK-3 were significantly lower after treating with 20 μg/mL BgIFITM3 protein (P < 0.05). Besides, the activities of HepG2 cells were significantly inhibited after treating with BgIFITM3 protein (1, 10 and 20 μg/mL) (P < 0.05). While, the cloning ability and migration ability of HepG2 cells were significantly inhibited after treating with 10 μg/mL BgIFITM3 protein (P < 0.05). Finally, the mitochondria of HepG2 cells was concentrated, cristae widened, and the double film density of mitochondria was increased after treating with 10 μg/mL BgIFITM3 protein. After 10 μg/mL BgIFITM3 protein treating, the expression levels of VDAC-2, VDAC-3 and p53 genes were significantly increased, but the expression level of GPX-4 gene was significantly decreased (P < 0.01). Taken together, the BgIFITM3 protein could inhibit the proliferations of yak hepatocyte and HepG2 cells by regulating the PI3K/Akt pathway or ferroptosis-related genes, respectively. These results benefit for further study of the function of BgIFITM3 protein.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.06.057DOI Listing
June 2021

Tbx18-positive cells-derived myofibroblasts contribute to renal interstitial fibrosis via transforming growth factor-β signaling.

Exp Cell Res 2021 Jun 9:112682. Epub 2021 Jun 9.

Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address:

It has been demonstrated that the T-box family transcription factor 18 (Tbx18) -positive cells give rise to renal mesenchymal cells and contribute to the development of the urinary system. However, it is unclear whether Tbx18-positive cells are the origin of the myofibroblasts during renal fibrosis. The present study aimed to determine the contribution of Tbx18-positive cells in kidney fibrosis and their underlying mechanism. We show that Tbx18-positivecells contribute to the development of the urinary system, especially renal fibroblasts. Following unilateral ureteral obstruction (UUO), genetic fate tracing results demonstrated that Tbx18-positive cells not only proliferate but also expand and differentiate into fibroblasts and myofibroblasts, indicating that they may act as profibrotic progenitors. Cell culture results suggest that transforming growth factor (TGF)-β promotes Tbx18-positive cells differentiation into myofibroblasts and assist their contribution to kidney fibrosis. Overall, the present study demonstrated that Tbx18-positive cells may act as profibrotic progenitor cells in a pathological condition of UUO-induced injury. Moreover, TGF-β may play a role in differentiation of Tbx18-positive cells into myofibroblasts in kidney fibrosis. These findings may provide a potential target on Tbx18-positive myofibroblast progenitors in the treatment of renal fibrosis.
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http://dx.doi.org/10.1016/j.yexcr.2021.112682DOI Listing
June 2021

First Isolation and Molecular Characterization of -Producing O157:H7 From Cattle in Xinjiang, China.

Front Vet Sci 2021 25;8:574801. Epub 2021 May 25.

College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, China.

The bovine O157:H7 is a major foodborne pathogen causing severe bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome in humans. Cattle are recognized major reservoir and source of O157:H7. We investigated the antibiotic resistance, molecular profiles, and intrinsic relationship between 21 isolates of O157:H7 from cattle farms and slaughtering houses in Xinjiang. Using pulsed-field gel electrophoresis (PFGE) molecular typing, two types of PFGE were revealed through cluster analysis, including clusters I and II, with 66 and 100% similarity of PFGE spectra between 21 isolates. We also detected that 18 isolates (86%) carried at least one virulence gene, 16 isolates (76%) carried the gene, and 7 (33%) carried the + + + + genes. Eighteen isolates were susceptible to antibiotics. Three isolates were resistant to antibiotics, and two were multidrug resistant. One of the two multidrug-resistant isolates detectably carried the gene. This is the first finding of the gene detected in O157:H7 isolated from cattle in Xinjiang. The gene is transferable between the bacterial strains via plasmid transmission. The results indicated that O157:H7 may have undergone clonal propagation in cattle population and cross-regional transmission in Xinjiang, China.
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http://dx.doi.org/10.3389/fvets.2021.574801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185162PMC
May 2021

LncRNAs serve as novel biomarkers for diagnosis and prognosis of childhood ALL.

Biomark Res 2021 Jun 10;9(1):45. Epub 2021 Jun 10.

Institute of Laboratory Medicine, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Key Laboratory of Big Data Mining and Precision Drug Design, School of Medical Technology, Guangdong Medical University, Guangdong Medical University, 523808, Dongguan, China.

Background: Although some studies have demonstrated that lncRNAs are dysregulated in hematopoietic malignancies and may regulate the progression of leukemia, the detailed mechanism underlying tumorigenesis is still unclear. This study aimed to investigate lncRNAs that are differentially expressed in childhood B-cell acute lymphoblastic leukemia (B-ALL) and T-cell acute lymphoblastic leukemia (T-ALL) and their potential roles in the progression of childhood ALL.

Methods: Microarrays were used to detect differentially expressed lncRNAs and mRNAs. Several aberrantly expressed lncRNAs were validated by qRT-PCR. Leukemia-free survival was analyzed using the Kaplan-Meier method with a log-rank test. The co-expression correlations of lncRNAs and mRNAs were determined by Spearman's correlation coefficient. CCK-8 assays and flow cytometry were performed to measure cell proliferation and apoptosis.

Results: We revealed that many lncRNAs were abnormally expressed in B-ALL and T-ALL. LncRNA/mRNA co-expression and the gene locus network showed that dysregulated lncRNAs are involved in diverse cellular processes. We also assessed the diagnostic value of the differentially expressed lncRNAs and confirmed the optimal combination of TCONS_00026679, uc002ubt.1, ENST00000411904, and ENST00000547644 with an area under the curve of 0.9686 [95 % CI: 0.9369-1.000, P < 0.001], with 90.7 % sensitivity and 92.19 % specificity, at a cut-off point of -0.5700 to distinguish childhood B-ALL patients from T-ALL patients, implying that these specific lncRNAs may have potential to detect subsets of childhood ALL. Notably, we found that the 8-year leukemia-free survival of patients with high TCONS_00026679 (p = 0.0081), ENST00000522339 (p = 0.0484), ENST00000499583 (p = 0.0381), ENST00000457217 (p = 0.0464), and ENST00000451368 (p = 0.0298) expression levels was significantly higher than that of patients with low expression levels of these lncRNAs, while patients with high uc002ubt.1 (p = 0.0499) and ENST00000547644 (p = 0.0451) expression levels exhibited markedly shorter 8-year leukemia-free survival. In addition, some lncRNAs were found to play different roles in cell proliferation and apoptosis in T-ALL and B-ALL.

Conclusions: Dysregulated lncRNAs involved in different regulatory mechanisms underlying the progression of childhood T-ALL and B-ALL might serve as novel biomarkers to distinguish ALL subsets and indicate poor outcomes.
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http://dx.doi.org/10.1186/s40364-021-00303-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193891PMC
June 2021

Knee Extensor Mechanism Strength and Its Relationship to Patellofemoral Kinematics in Individuals With Arthrofibrosis Within 6 Months After Anterior Cruciate Ligament Reconstruction.

J Sport Rehabil 2021 Jun 10:1-6. Epub 2021 Jun 10.

Context: Performance in strength and assessment of patellar tracking is important for patients with arthrofibrosis after anterior cruciate ligament (ACL) reconstruction.

Objective: The study was to examine the difference of patellofemoral kinematics between the affected and the contralateral limb and to evaluate the relationship between knee extensor strength and patellofemoral kinematics in patients with arthrofibrosis after ACL reconstruction.

Design: Cohort study (diagnosis); level of evidence, 3.

Setting: Laboratory.

Patients: A prospective cohort of 20 patients with arthrofibrosis after ACL reconstruction was recruited.

Interventions: A total of 20 patients who underwent arthroscopic reconstruction of the double-bundle ACL with a hamstring tendon autograft received standardized patellofemoral kinematics testing and knee extensor strength testing within 6 months after primary ACL reconstruction. Computed tomography and dual fluoroscopic imaging were used to evaluate in vivo patellofemoral kinematics of affected and contralateral knees during a lunge task. Knee extensor mechanism strength was measured using a handheld dynamometer.

Main Outcome Measures: A limb symmetry index of knee strength and patellar mobility was calculated and satisfactory performance defined as ≥90%.

Results: There was a statistically significant decrease in the range of patellar inferior shift (P = .020; d = 0.81), flexion (P = .026; d = 0.95), lateral tilt (P = .001; d = 1.04), and lateral rotation (P < .001; d = 0.89) in the affected knee compared with the contralateral knee from 15° to 75° of knee flexion. There was a strong positive linear correlation between knee extensor strength and patellar inferior shift (r = .747; P = .008). A knee extensor strength limb symmetry index <90% was 89% sensitive and 9% specific for limited patellar inferior shift.

Conclusions: Patients with arthrofibrosis after ACL reconstruction presented decreased patellar mobility in the arthrofibrotic knee compared with the contralateral knee. The strong correlation between knee extensor strength and patellar inferior shift of the arthrofibrotic knee demonstrates the importance of knee extensor strength in the diagnosis and treatment of patients with knee arthrofibrosis. The knee extensor mechanism strength has high sensitivity but low specificity in identifying a decrease in patellar inferior shift in patients with arthrofibrosis after ACL reconstruction.
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http://dx.doi.org/10.1123/jsr.2020-0468DOI Listing
June 2021

Relating self-other overlap to ingroup bias in emotional mimicry.

Soc Neurosci 2021 Jun 13:1-9. Epub 2021 Jun 13.

Department of Psychology, Renmin University of China, Beijing, P.R. China.

Humans tend to show congruent facial expressions automatically in reaction to their partners which is defined as emotional mimicry. Although occurring unconsciously, this tendency has been proven to be modulated by social contextual factors such as group membership. Ingroup bias in emotional mimicryhas been well-documented in previous research; however, few studies have investigated the underlying mechanism. Based on the mimicry-as-social-regulator model, this study explored whether the ingroup bias in emotional mimicry arises from the greater self-ingroup overlap. By recording participants' facial electromyographic responses while passively viewing dynamic emotional clips performed by either racial ingroup or outgroup actors, Study 1 validated the presence of ingroup bias in the mimicry of happiness, but not anger. Using asimilar procedure in Study 2, anew sample was employed ( = 37), and a measurement of self-other overlaps via the Inclusion of the Other in the Self Scale was added. The results of Study 2 reproduced the ingroup bias in happy mimicry, and further demonstrated that the effect of group membership on emotional mimicry was mediated by the self-other overlap. In summary, this study provides evidence that the level of interpersonal closeness predicts emotional mimicry.
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http://dx.doi.org/10.1080/17470919.2021.1940273DOI Listing
June 2021

miR-224-5p Carried by Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes Regulates Autophagy in Breast Cancer Cells via HOXA5.

Front Cell Dev Biol 2021 21;9:679185. Epub 2021 May 21.

Department of Clinical Laboratory Medicine, Taizhou Central Hospital (Taizhou University Hospital), Taizhou City, China.

In this study, we focused on the potential mechanism of miRNAs carried by human umbilical cord mesenchymal stem cells-derived exosomes (hUCMSCs-exo) in breast cancer (BC). RT-qPCR was conducted for the expression of miR-224-5p and HOXA5 in tissues and cells. After co-culture of exosomes and MCF-7 or MDA-MB-231 cells, the cell proliferation was observed by MTT and cell colony formation assay, while apoptosis was measured by flow cytometry. In addition, the expression of HOXA5 and autophagy pathway-related proteins LC3-II, Beclin-1 and P62 was detected by western blotting. And immunofluorescence was applied for detection of LC3 spots. The binding of miR-224-5p to HOXA5 was verified by the luciferase reporter gene assay and RNA-binding protein immunoprecipitation assay. Finally, experiment was performed to investigate the effect of miR-224-5p on BC growth. MiR-224-5p was up-regulated and HOXA5 was down-regulated in BC tissues and cells. HOXA5 was confirmed to be the target gene of miR-224-5p. MiR-224-5p carried by hUCMSCs-exo was able to promote the proliferation and autophagy of BC cells, while inhibited apoptosis. Bases on xenograft models in nude mice, it was also revealed that miR-224-5p carried by hUCMSCs-exo could regulate autophagy and contribute to the occurrence and development of BC . MiR-224-5p carried by hUCMSCs-exo can regulate autophagy via inhibition of HOXA5, thus affecting the proliferation and apoptosis of BC cells.
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http://dx.doi.org/10.3389/fcell.2021.679185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176026PMC
May 2021

Photosynthetic and Photoprotective Responses to Steady-State and Fluctuating Light in the Shade-Demanding Crop Grown in Intercropping and Monoculture Systems.

Front Plant Sci 2021 21;12:663473. Epub 2021 May 21.

College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.

Photosynthetic and photoprotective responses to simulated sunflecks were examined in the shade-demanding crop intercropped with maize (intercropping condition) or grown in an adjacent open site (monoculture condition). Both intercropping leaves and monoculture leaves exhibited very fast induction responses. The times taken to achieve 90% maximum net photosynthetic rate in intercropping leaves and monoculture leaves were 198.3 ± 27.4 s and 223.7 ± 20.5 s during the photosynthetic induction, respectively. During an 8-min simulated sunfleck, the proportion of excess excited energy dissipated through the xanthophyll cycle-dependent pathway () and dissipated through constitutive thermal dissipation and the fluorescence () pathway increased quickly to its maximum, and then plateaued slowly to a steady state in both intercropping and monoculture leaves. When the illumination was gradually increased within photosystem II (PSII), increased quicker and to a higher level in monoculture leaves than in intercropping leaves. Relative to their monoculture counterparts, intercropping leaves exhibited a significantly lower accumulation of oxygen free radicals, a significantly higher content of chlorophyll, and a similar content of malondialdehyde. Although monoculture leaves exhibited a larger mass-based pool size of xanthophyll cycle [V (violaxanthin) + A (antheraxanthin) + Z (zeaxanthin)] than intercropping leaves, intercropping leaves had a higher ratio of (Z + A)/(V + Z + A) than monoculture leaves. intercropping leaves had markedly higher glutathione content and ascorbate-peroxidase activity than their monoculture counterparts. Similar activities of catalase, peroxidase, dehydroascorbate reductase, and monodehydroascorbate were found in both systems. Only superoxide dismutase activity and ascorbate content were lower in the intercropping leaves than in their monoculture counterparts. Overall, the xanthophyll cycle-dependent energy dissipation and the enzymatic antioxidant defense system are important for protecting plants from photooxidation in an intercropping system with intense sunflecks.
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http://dx.doi.org/10.3389/fpls.2021.663473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175988PMC
May 2021

Prevalence of Post-traumatic Stress Symptoms and Its Associations With Quality of Life, Demographic and Clinical Characteristics in COVID-19 Survivors During the Post-COVID-19 Era.

Front Psychiatry 2021 21;12:665507. Epub 2021 May 21.

Unit of Psychiatry, Department of Public Health and Medicinal Administration, Faculty of Health Sciences, University of Macau, Macao SAR, China.

The prevalence of post-traumatic stress symptoms (PTSS) in COVID-19 survivors is unclear. This study examined the prevalence of PTSS and its association with quality of life (QOL) among COVID-19 survivors during the post-COVID-19 era in China. This was a comparative, cross-sectional study. PTSS, depressive symptoms, and QOL were assessed with standardized instruments. A total of 134 COVID-19 survivors and 214 non-infected controls (healthy controls hereafter) were recruited. Among COVID-19 survivors, the PTSS prevalence was 18.66% (95%CI: 11.98-25.34%), which was significantly higher than that (5.61%, 95%CI: 2.50-8.71%) of healthy controls ( < 0.001). After controlling for covariates, an analysis of covariance (ANCOVA) showed that COVID-19 survivors had a higher PTSS total score than did healthy controls [ = 4.664, = 0.032]. A separate ANCOVA revealed there were no significant differences in overall QOL between COVID-19 survivors with and without PTSS [ = 1.067, = 0.304]. A multiple logistic regression analysis showed that more severe depressive symptoms were significantly associated with PTSS in COVID-19 survivors (OR = 1.425, < 0.001). PTSS were more severe in COVID-19 survivors compared to healthy controls in the post-COVID-19 era. Considering their negative impact on daily life and functional outcomes, regular assessment and appropriate treatments of PTSS should be conducted in COVID-19 survivors.
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http://dx.doi.org/10.3389/fpsyt.2021.665507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176302PMC
May 2021

Association study of fasting blood glucose and salt sensitivity of blood pressure in community population: The EpiSS study.

Nutr Metab Cardiovasc Dis 2021 May 8. Epub 2021 May 8.

Department of Epidemiology and Health Statistics, School of Public Health, Capital Medical University, and Beijing Municipal Key Laboratory of Clinical Epidemiology Beijing, 100069, China. Electronic address:

Background And Aims: To evaluate the association between fasting blood glucose (FBG) and salt sensitivity of blood pressure (SSBP).

Methods And Results: This study is based on the baseline survey of systemic epidemiology of salt sensitivity study. Subjects were classified into salt sensitive (SS) and salt resistant groups according to blood pressure (BP) changes during the modified Sullivan's acute oral saline load and diuresis shrinkage test. Multivariate logistic and linear regression were used to evaluate associations between FBG with SS or BP changes. A total of 2051 participants were included in the analyses with 581 (28.33%) for SS. Multiple analysis showed that for every interquartile range increase in FBG, the OR (95%CI) for SS was 1.140 (1.069, 1.215), β (95%CI) for mean arterial pressure change (ΔMAP), systolic and diastolic BP changes during saline load were 0.421 (0.221, 0.622), 0.589 (0.263, 0.914) and 0.340 (0.149, 0.531), respectively. Compared to the lowest FBG quartile (Q), the OR (95%CI) for SS in Q and Q were 1.342 (1.014, 1.776) and 1.577 (1.194, 2.084), respectively. Compared to subjects with normal FBG, the β (95%CI) for ΔMAP was 0.973 (0.055, 1.891) in subjects with impaired FBG, and was 1.449 (0.602, 2.296) in patients with diabetes mellitus. Stratified analyses showed significant and stronger associations between FBG with SSBP in youngers, females, hypertensives, non-diabetics, non-current smokers and non-current drinkers.

Conclusion: Our findings suggest FBG is an independent, dose-dependent associated factor for SSBP, and prevention of SS focusing on controlling FBG elevation in the early stage is important.
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http://dx.doi.org/10.1016/j.numecd.2021.04.026DOI Listing
May 2021

Matrine suppresses cell growth of diffuse large B-cell lymphoma via inhibiting CaMKIIγ/c-Myc/CDK6 signaling pathway.

BMC Complement Med Ther 2021 Jun 4;21(1):163. Epub 2021 Jun 4.

The First Affiliated Hospital, Zhejiang Chinese Medical University, No. 54 Youdian Road, Hangzhou, 310006, Zhejiang, China.

Background: C-Myc aberrations confer a more aggressive clinic behavior in diffuse large B-cell lymphoma (DLBCL). Matrine is an alkaloid extracted from Sophora flavescens Ait. It possesses anti-cancer property through inhibiting the cell proliferation and inducing the apoptosis. The present study aimed to explore the underlying mechanisms of matrine in suppressing the cell growth of DLBCL.

Methods: The influence of matrine on the viability of cultured DLBCL cell lines SU-DHL-16 and OCI-LY3 cells were determined by CCK-8. Apoptosis and cell cycle were measured by flow cytometry after matrine exposure. Western blot was taken to investigate the expression of activated Caspase-3, cleaved PARP, c-Myc, phospho-c-Myc (Ser62), CaMKIIγ, phospho-CaMKIIγ (Thr287), CDK4 and CDK6 after matrine treatment. Cycloheximide chase analysis was used to determine the c-Myc protein half-lives before and after matrine treatment. Growth salvage analysis was taken by ectopic expression of c-Myc.

Results: In cultured DLBCL cells, matrine suppressed cell viability in a concentration and time dependent fashion. Matrine treated SU-DHL-16 and OCI-LY3 cells for 48 h with IC value of 1.76 mM and 4.1 mM, respectively. Matrine induced apoptosis through a caspase-independent pathway and caused G/G cell cycle arrest in a concentration dependent manner in DLBCL cells. The protein expression of c-Myc was inhibited while the transcription of c-Myc was not reduced by matrine. c-Myc protein half-lives were decreased from 30.4, 69.4 min to 16.6, 15.9 min after matrine treatment in SU-DHL-16 and OCI-LY3, respectively. As a critical protein kinase of c-Myc, CaMKIIγ phosphorylation at Thr287 was found to be down-regulated and c-Myc phosphorylation at Ser62 was reduced together after matrine treatment in DLBCL. The growth suppression of SU-DHL-16 cells induced by matrine was rescued by over-expression of c-Myc achieved by recombinant adenovirus infection. The decreased expression of CDK6, not CDK4, induced by matrine was rescued by ectopic expression of c-Myc protein.

Conclusions: This study has shown for the first time that matrine suppresses cell growth of DLBCL via inhibiting CaMKIIγ/c-Myc/CDK6 signaling pathway.
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http://dx.doi.org/10.1186/s12906-021-03315-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178855PMC
June 2021

Preparation and characterization of konjac glucomannan and gum arabic composite gel.

Int J Biol Macromol 2021 Jun 1;183:2121-2130. Epub 2021 Jun 1.

College of Food Science and Engineering, Jilin University, Changchun 130062, China; Electron Microscopy Center, Jilin University, Changchun 130012, China.

Compounding is a safe method to avoid limitations of a singular gel. Here, composite gels were prepared with konjac glucomannan (KGM) and gum arabic (GA) and evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), water solubility index (WSI), water absorption index (WAI), texture profile analysis (TPA) and rheological analysis. The gel stratified when GA concentration ≥ 2.5%. FTIR indicated that the interactions of KGM and GA were mainly related to hydrogen bonds and acetyl groups, and the solution separated from gels only included GA and water molecules. The microstructures became denser and contained smaller holes at high GA concentrations as seen by SEM. WSI and WAI both increased with GA increasing. Hardness and springiness dropped when GA concentration increased from 0 to 2.0%, but they increased when GA increased from 2.0% to 4.0%. Rheological analysis showed the gels were non-Newtonian pseudoplastic fluids, with anti-thixotropy (GA ≤ 3.5%) and thixotropy (GA ≥ 4.0%). Furthermore, the gels could be classified as non-covalent gels, with higher gel strength at high GA concentrations. The non-covalent linkages included hydrogen bonding and hydrophobic interaction, and hydrogen bonding held the dominated status. Therefore, KGM and GA have antagonistic and synergistic effects.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.196DOI Listing
June 2021

Novel brain-targeting 3-n-butylphthalide prodrugs for ischemic stroke treatment.

J Control Release 2021 Jun 1;335:498-514. Epub 2021 Jun 1.

West China School of Pharmacy, College of Polymer Science and Engineering, Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, Sichuan University, Chengdu 610064, PR China. Electronic address:

Currently, ischemic stroke is the leading cause of disability and death worldwide, and the performance of corresponding drugs is often unsatisfactory owing to the complex pathological processes and the impediment of the blood-brain barrier (BBB). Here, we employed various tertiary amino groups, including different linear, cyclic, and bimolecular drug structures, to modify 3-n-butylphthalide (NBP), a natural product used for ischemic stroke treatment, which has poor bioavailability, to generate a series of six prodrugs. These prodrugs showed significantly improved solubility and cellular uptake, which were primarily driven by putative pyrilamine cationic transporters. They also displayed more efficient brain delivery in vivo, reaching as high as 21.5-fold brain accumulation increase compared with NBP, leading to much higher bioavailability and stronger therapeutic effects. The toxicity of these molecules is also lower or similar to that of unmodified NBP. We showed that the tertiary amino group-modified NBP prodrugs are effective and safe for treating ischemic stroke with significantly enhanced druggability; hence, they have potential for further clinical development.
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http://dx.doi.org/10.1016/j.jconrel.2021.05.045DOI Listing
June 2021

Amelioration of Beta Interferon Inhibition by NS4B Contributes to Attenuating Tembusu Virus Virulence in Ducks.

Front Immunol 2021 17;12:671471. Epub 2021 May 17.

Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, China.

Our previous studies reported that duck Tembusu virus nonstructural protein 2A (NS2A) is a major inhibitor of the IFNβ signaling pathway through competitively binding to STING with TBK1, leading to a reduction in TBK1 phosphorylation. Duck TMUV NS2B3 could cleave and bind STING to subvert the IFNβ signaling pathway. Here, we found that overexpression of duck TMUV NS4B could compete with TBK1 in binding to STING, reducing TBK1 phosphorylation and inhibiting the IFNβ signaling pathway by using the Dual-Glo Luciferase Assay System and the NanoBiT protein-protein interaction (PPI) assay. We further identified the E2, M3, G4, W5, K10 and D34 residues in NS4B that were important for its interaction with STING and its inhibition of IFNβ induction, which were subsequently introduced into a duck TMUV replicon and an infectious cDNA clone. We found that the NS4B M3A mutant enhanced RNA replication and exhibited significantly higher titer levels than WT at 48-72 hpi but significantly decreased mortality (80%) in duck embryos compared to WT (100%); the NS4B G4A and R36A mutants slightly reduced RNA replication but exhibited the same titer levels as WT. However, the NS4B R36A mutant did not attenuate the virulence in duck embryos, whereas the G4A mutant significantly decreased the mortality (70%) of duck embryos. In addition, the NS4B W5A mutant did not affect viral replication, whereas the D34A mutant slightly reduced RNA replication, and both mutants exhibited significantly lower titer levels than the WT and significantly decreased mortality (90% and 70%, respectively) in duck embryos. Hence, our findings provide new insight into the development of attenuated flaviviruses by targeting the disabling viral strategies used to evade the innate defense mechanisms.
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http://dx.doi.org/10.3389/fimmu.2021.671471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165282PMC
May 2021

Branched Aggregates with Tunable Morphology via Hierarchical Self-Assembly of Azobenzene-Derived Molecular Double-Brushes.

Angew Chem Int Ed Engl 2021 Jun 1. Epub 2021 Jun 1.

East China University of Science and Technology, School of Materials Science and Engineering, Meilong Road 130, 200237, Shanghai, CHINA.

Hierarchical self-assembly has been one of the most effective approach to fabricate nature-inspired materials with subtle nanostructures and variable functions. Herein, we report a distinct hierarchical self-assembly process of azobenzene-derived Janus molecular double-brushes (MDBs) with each graft site carrying a poly(azobenzene-acrylate) (PAzo) chain and a poly(ethylene oxide) (PEO) chain. Asymmetric tapered-worm (ATW) nanostructures with chain-end reactivity assembling from the azobenzene-derived MDBs serve as primary subunits to prepare branched supermicelles by increasing water content (Cw) in THF/water, which is predominantly facilitated by the distinctive molecular brush architecture and the intermolecular interaction of PAzo brushes. Various natural antedon-shaped multiarm worm-like aggregates (MWAs) can be created depending on Cw via the particle-particle connection of ATWs. Intriguingly, the azobenzene moieties undergo trans-cis  isomerization upon UV irradiation and further promote a morphology evolution of MWAs, leading the formation of starfish-shaped aggregates. Subsequently, multiscale luidia ciliaris and crossaster papposus-shaped supermicelles with different features of central body and arms are prepared by manipulating irradiation time.
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http://dx.doi.org/10.1002/anie.202106321DOI Listing
June 2021

Association of Lipoprotein (a) variants with risk of cardiovascular disease: a Mendelian randomization study.

Lipids Health Dis 2021 Jun 1;20(1):57. Epub 2021 Jun 1.

Department of Epidemiology and Health Statistics, School of Public Health, Capital Medical University and Beijing Municipal Key Laboratory of Clinical Epidemiology, No 10 Xitoutiao, You'anmenwai, Fengtai, Beijing, 100069, P. R. China.

Background: There is a well-documented empirical relationship between lipoprotein (a) [Lp(a)] and cardiovascular disease (CVD); however, causal evidence, especially from the Chinese population, is lacking. Therefore, this study aims to estimate the causal association between variants in genes affecting Lp(a) concentrations and CVD in people of Han Chinese ethnicity.

Methods: Two-sample Mendelian randomization analysis was used to assess the causal effect of Lp(a) concentrations on the risk of CVD. Summary statistics for Lp(a) variants were obtained from 1256 individuals in the Cohort Study on Chronic Disease of Communities Natural Population in Beijing, Tianjin and Hebei. Data on associations between single-nucleotide polymorphisms (SNPs) and CVD were obtained from recently published genome-wide association studies.

Results: Thirteen SNPs associated with Lp(a) levels in the Han Chinese population were used as instrumental variables. Genetically elevated Lp(a) was inversely associated with the risk of atrial fibrillation [odds ratio (OR), 0.94; 95% confidence interval (95%CI), 0.901-0.987; P = 0.012)], the risk of arrhythmia (OR, 0.96; 95%CI, 0.941-0.990; P = 0.005), the left ventricular mass index (OR, 0.97; 95%CI, 0.949-1.000; P = 0.048), and the left ventricular internal dimension in diastole (OR, 0.97; 95%CI, 0.950-0.997; P = 0.028) according to the inverse-variance weighted method. No significant association was observed for congestive heart failure (OR, 0.99; 95% CI, 0.950-1.038; P = 0.766), ischemic stroke (OR, 1.01; 95%CI, 0.981-1.046; P = 0.422), and left ventricular internal dimension in systole (OR, 0.98; 95%CI, 0.960-1.009; P = 0.214).

Conclusions: This study provided evidence that genetically elevated Lp(a) was inversely associated with atrial fibrillation, arrhythmia, the left ventricular mass index and the left ventricular internal dimension in diastole, but not with congestive heart failure, ischemic stroke, and the left ventricular internal dimension in systole in the Han Chinese population. Further research is needed to identify the mechanism underlying these results and determine whether genetically elevated Lp(a) increases the risk of coronary heart disease or other CVD subtypes.
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http://dx.doi.org/10.1186/s12944-021-01482-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170931PMC
June 2021

Association of long-term exposure to ambient particulate pollution with stage 1 hypertension defined by the 2017 ACC/AHA Hypertension Guideline and cardiovascular disease: The CHCN-BTH cohort study.

Environ Res 2021 Aug 26;199:111356. Epub 2021 May 26.

Department of Epidemiology and Health Statistics, School of Public Health, Capital Medical University, And Beijing Municipal Key Laboratory of Clinical Epidemiology, Beijing, China. Electronic address:

Background: Evidence regarding the effects of ambient air pollution on new stage 1 hypertension defined by the 2017 ACC/AHA Hypertension Guideline remains sparse.

Objectives: To investigate the association of long-term exposure to ambient PM with stage 1 hypertension and to explore the mediating and modifying effects of PM on cardiovascular disease (CVD).

Methods: A total of 32,135 participants aged 18-80 years were recruited in 2017. The three-year (2014-2016) average PM concentrations were assessed by a spatial statistical model. Blood pressure (BP) was divided into four categories according to the 2017 ACC/AHA Hypertension Guideline: normal BP (SBP<120 mmHg and DBP<80 mmHg), elevated BP (SBP 120-129 mmHg and DBP<80 mmHg), stage 1 hypertension (SBP 130-139 mmHg or DBP 80-89 mmHg), and stage 2 hypertension (SBP≥140 mmHg or DBP≥90 mmHg or taking antihypertensive medications). The associations of PM with BP categories were estimated by two-level generalized linear mixed models. Analyses stratified by age, mediation and interaction analyses of PM and stage 1 hypertension with CVD were performed.

Results: We detected a positive significant association between long-term exposure to PM and stage 1 hypertension. Compared to normal BP, the OR was 1.05 (95% CI: 1.02, 1.08) per 10 μg/m increase in PM. The association was stronger than that of elevated BP but weaker than that of stage 2 hypertension. Stage 1 hypertension only partially mediated the association between PM and CVD, and the mediation proportions ranged from 1.55% to 11.00%. However, it modified the association between PM and CVD, which was greater in participants with stage 1 hypertension (OR: 1.66; 95% CI: 1.43, 1.93) than in participants with normal BP (OR: 1.32; 95% CI: 1.11, 1.57), with P<0.001. In the analysis stratified by age, the above associations were age-specific, and significant associations were only observed in the young and middle-aged (<60 years) groups.

Conclusions: Long-term exposure to ambient PM was significantly associated with stage 1 hypertension. This earlier stage of hypertension may be a trigger BP range for adverse effects of air pollution in the development of hypertension and CVD, especially in young and middle-aged individuals.
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http://dx.doi.org/10.1016/j.envres.2021.111356DOI Listing
August 2021

"Superaerophobic" NiCo bimetallic phosphides for highly efficient hydrogen evolution reaction electrocatalysts.

Chem Commun (Camb) 2021 Jun;57(50):6173-6176

The State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing Key Laboratory of Chemical Process for Clean Energy and Resource Utilization, School of Chemistry and Chemical Engineering, Chongqing University, Shazhengjie 174, Chongqing 400044, China.

A "superaerophobic" NiCo bimetallic phosphide electrocatalyst has been fabricated by employing bimetal-organic frameworks as self-sacrificing templates. An overpotential of only 205 mV can drive the HER current density to 800 mA cm-2, which is even superior to that for Pt/C. This study provides a promising approach for the development of industrialized HER electrocatalysts.
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http://dx.doi.org/10.1039/d1cc01698aDOI Listing
June 2021

Correlation analysis of neck node levels in 960 cases of Nasopharyngeal carcinoma (NPC).

Radiother Oncol 2021 May 25;161:23-28. Epub 2021 May 25.

Department of Oncology, The General Hospital of Western Theater Command, Chengdu, PR China. Electronic address:

Purpose: The delineation of intermediate risk nodal regions (CTVn2) and low-risk nodal regions (CTVn3) base on the correlation analysis between neck node levels of NPC has not been reported. We aim to analyze the correlations between different neck node levels in 960 cases of NPC, and to provide preliminary suggestions for clinical target volume (CTV) delineation of NPC base on the correlation analysis.

Methods And Materials: We retrospectively analyzed the records of 960 NPCs in our institution from 2011 to 2019. Diagnostic head and neck CTs and MRIs were reviewed. The involvements of nodal levels were evaluated according to the 2013 updated guidelines. The correlations between different levels were studied using Chi-square test and logistic regression model.

Results: The top four levels with the highest rate of lymph node metastasis were VIIa(86.35%), IIb(84.06%), IIa(62.29%), and III(47.29%). Correlation analysis showed that lymph node metastasis in level Ib was correlated with levels IIa and III. Level IIa was correlated with levels Ib, IIb, III, and Va. Level IIb was correlated with levels IIa, III, Va, and VIIa. Level III was correlated with levels IIa, IIb, IVa, Va, Vb, VIIa, and T stage. Level IVa was correlated with levels III, IVb, and Va. Level IVb was only correlated with level IVa. Level Va was correlated with levels IIb, III, IVa, Vb, and posterior to level V (PLV region). Level Vb was correlated with levels III, Va, Vc, and PLV region. Level Vc was correlated with levels IVb, Vb, and PLV region. Level VIIa was correlated with levels IIb and III. Level VIIb had no correlations with other levels. Level VIII was correlated with levels Ib and IVb. The PLV region was correlated with levels Va, Vb, and Vc. All the above P values were <0.05.

Conclusions: This study recommends setting the related levels as CTVn2 and the unrelated levels as CTVn3. The levels of nodal spread are different in NPC patients, this study reflects the principle of individualized CTV delineation.
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http://dx.doi.org/10.1016/j.radonc.2021.05.020DOI Listing
May 2021