Publications by authors named "Ling Wang"

3,380 Publications

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MAB21L1 promotes survival of lens epithelial cells through control of αB-crystallin and ATR/CHK1/p53 pathway.

Aging (Albany NY) 2022 Aug 10;14(undefined). Epub 2022 Aug 10.

College of Life Sciences, Hunan Normal University, Changsha 410080, Hunan, China.

The male abnormal gene family 21 (), was initially identified in . Since its identification, studies from different groups have shown that it regulates development of ocular tissues, brain, heart and liver. However, its functional mechanism remains largely unknown. Here, we demonstrate that Mab21L1 promotes survival of lens epithelial cells. Mechanistically, Mab21L1 upregulates expression of αB-crystallin. Moreover, our results show that αB-crystallin prevents stress-induced phosphorylation of p53 at S-20 and S-37 through abrogating the activation of the upstream kinases, ATR and CHK1. As a result of suppressing p53 activity by αB-crystallin, Mab21L1 downregulates expression of Bak but upregulates Mcl-1 during stress insult. Taken together, our results demonstrate that Mab21L1 promotes survival of lens epithelial cells through upregulation of αB-crystallin to suppress ATR/CHK1/p53 pathway.
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http://dx.doi.org/10.18632/aging.204203DOI Listing
August 2022

Specific brain gray matter volume changes in pediatric complete spinal cord injury without fracture or dislocation using voxel-based morphometry analysis: Preliminary Results.

J Neurotrauma 2022 Aug 11. Epub 2022 Aug 11.

Xuanwu Hospital Capital Medical University, Department of Radiology and Nuclear medicine, Beijing, China.

This study aims to investigate the brain gray matter volume (GMV) alterations of pediatric complete thoracolumbar spinal cord injury without fracture or dislocation (SCIWOFD) using voxel-based morphometry (VBM) analysis and assess the sensitive neuroimaging biomarkers which may be surrogate targets to enhance brain plasticity. A total of 52 pediatric subjects (age range, 6-12 years), including 25 pediatric SCIWOFD patients and 27 typically developing (TD) children were recruited. Independent two-sample t test was performed to assess between-group differences of brain GMV. Partial correlation analyses were performed to explore the correlations between GMV values and ISNCSCI scores, age at the time of injury, time after initial SCI. Receiver operating characteristic (ROC) analysis was performed to compute the sensitivity and specificity of the imaging biomarkers for pediatric SCIWOFD diagnosis. As the results, pediatric SCIWOFD patients showed significantly decreased GMV of bilateral Cerebellum lobule VIII, right middle occipital gyrus (MOG) and putamen (PUT), left pallidum (PAL) and thalamus (THA), and increased GMV of Vermis_III, right Cerebellum lobule VI and SupraMarginal gyrus (SMG). Additionally, GMV of left PAL and right PUT were negatively correlated with the pinprick/light touch sensory scores in pediatric SCIWOFD patients. Finally, when using the GMV values of left PAL and right PUT in combination as the predictor, area under the curve (AUC) reached the highest, of 0.93. These findings provided evidence that the brain undergoes GMV changes following pediatric SCIWOFD, which may suggest important targets for functional remodeling after SCI in children and provide valuable information for the development of novel and effective rehabilitation therapies in the future.
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http://dx.doi.org/10.1089/neu.2022.0247DOI Listing
August 2022

Opioid prescriptions for individuals receiving workers' compensation in Michigan.

PLoS One 2022 9;17(8):e0272385. Epub 2022 Aug 9.

Department of Medicine, Michigan State University, East Lansing, Michigan, United States of America.

Purpose: We evaluated the prevalence of opioid prescriptions after injury and associated characteristics among workers receiving workers' compensation for a lost work time injury.

Methods: Injured workers identified in Michigan's Workers' Compensation records from 2016 to 2018 were linked to the opioid prescription history in the Michigan Automated Prescription System.

Results: Among the 46,934 injured workers with paid claims, the prevalence of receiving an opioid prescription, morphine milligram equivalents (MME) per prescription, number of opioid prescription and probability of receiving opioids prescription>90 days after injury decreased from 2016-2018. Despite the decrease over 50% of the injured workers received an opioid prescription. Being over 34 years, a male, having had an opioid prescription before the injury, working in construction or having an amputation or sprain/strain of the shoulder had a significantly higher probability of receiving an opioid prescription, a higher MME per prescription, a higher number of opioid prescriptions and a higher probability having opioids prescription >90 days after the injury.

Conclusions: Even though opioid prescribing patterns generally decreased from 2016 to 2018 (64.5-52.8%), injured workers in Michigan had a higher prevalence of opioid prescription after injury, than those reported from other states.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0272385PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9362907PMC
August 2022

Poly(,-dimethyl)acrylamide-based ion-conductive gel with transparency, self-adhesion and rapid self-healing properties for human motion detection.

Soft Matter 2022 Aug 9. Epub 2022 Aug 9.

Key Laboratory of Polymeric Materials Design and Synthesis for Biomedical Function, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.

Flexible strain sensors have been extensively studied for their potential value in monitoring human activity and health. However, it is still challenging to develop multifunctional flexible strain sensors with simultaneously high transparency, strong self-adhesion, fast self-healing and excellent tensile properties. In this study, we used ,-dimethylacrylamide (DMA) in the imidazolium-based ionic liquid 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl] imide ([BMIM][TfN]) for "one-step" UV irradiation. A poly(,-dimethyl)acrylamide (PDMA) ion-conductive gel was prepared by site polymerization. Based on the good compatibility between PDMA and ionic liquid, the prepared ion-conductive gel has good transparency (∼90%), excellent stretchability (1080%), strong self-adhesion (67.57 kPa), fast self-healing (2 s at room temperature) and great antibacterial activity (∼99% bacterial killing efficiency). Moreover, the strain sensor based on the PDMA ion-conductive gel has good electromechanical performance and can detect different human motions. Based on the simple and easy-to-operate preparation method and the endowed multifunctionality of the PDMA ion-conductive gel, it has broad application prospects in the field of flexible electronic devices.
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http://dx.doi.org/10.1039/d2sm00786jDOI Listing
August 2022

Dynamic control of pentachlorophenol photodegradation process using P25/PDA/BiOBr through regulation of photo-induced active substances and chemiluminescence.

Chemosphere 2022 Aug 5;307(Pt 2):135914. Epub 2022 Aug 5.

State Key Laboratory of Environmental Chemistry and Eco-toxicology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; University of Chinese Academy of Sciences, Beijing, 100049, China; School of Environment, Hangzhou Institute for Advanced Study, UCAS, Hangzhou, 310000, China.

Photodegradation is a new approach for the removal of pentachlorophenol (PCP). Photooxidation degradation (using hydroxyl radicals) exhibits better performance to remove PCP than photoreduction degradation, but the former will lead to an increase in the production of toxic by-products such as tetrachloro-1,4-benzoquinone (TCBQ). Thus, a new strategy is required to enhance PCP photodegradation and simultaneously inhibit toxic intermediates production. Herein, TiO (P25)/polydopamine (PDA)/BiOBr was synthesized and used to photodegrade PCP. Based on the relative position of the CB and VB of P25 and BiOBr, and PDA as an electron transfer mediator, a high number of holes, electrons, and superoxide anions were produced instead of hydroxyl radicals. The photocatalytic activity of P25/PDA/BiOBr exhibited the best performance among as-prepared samples, reaching a k value of 0.4 min (20 μM PCP) under UV light irradiation within 10 min. According to chemiluminescence and acute toxicity assays, relative to P25, the toxic intermediates of TCBQ and trichlorohydroxy-1,4-benzoquinone (OH-TrCBQ) generation was greatly reduced over P25/PDA/BiOBr, with a lack of toxic product generation during PCP photodegradation process. These findings provide an alternative strategy to achieve greener and more efficient organic pollutant photodegradation.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135914DOI Listing
August 2022

3D-printed high-density polyethylene scaffolds with bioactive and antibacterial layer-by-layer modification for auricle reconstruction.

Mater Today Bio 2022 Dec 15;16:100361. Epub 2022 Jul 15.

Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China.

High-density polyethylene (HDPE) is a promising material for the development of scaffold implants for auricle reconstruction. However, preparing a personalized HDPE auricle implant with favorable bioactive and antibacterial functions to promote skin tissue ingrowth is challenging. Herein, we present 3D-printed HDPE auricle scaffolds with satisfactory pore size and connectivity. The layer-by-layer (LBL) approach was applied to achieve the improved bioactive and antibacterial properties of these 3D printed scaffolds. The HDPE auricle scaffolds were fabricated using an extrusion 3D printing approach, and the individualized macrostructure and porous microstructure were both adjusted by the 3D printing parameters. The polydopamine (pDA) coating method was used to construct a multilayer ε-polylysine (EPL) and fibrin (FIB) modification on the surface of the 3D HDPE scaffold the LBL self-assembly approach, which provides the bioactive and antibacterial properties. The results of the experiments using an animal model showed that LBL-coated HDPE auricular scaffolds were able to significantly enhance skin tissue ingrowth and ameliorate the inflammatory response caused by local stress. The results of this study suggest that the combination of the 3D printing technique and surface modification provides a promising strategy for developing personalized implants with biofunctional coatings, which show great potential as a scaffold implant for auricle reconstruction applications.
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http://dx.doi.org/10.1016/j.mtbio.2022.100361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352972PMC
December 2022

Changes and Correlation Between Physiological Characteristics of and Soil Microbial Communities Under Heat Stress.

Front Plant Sci 2022 22;13:950947. Epub 2022 Jul 22.

Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education/College of Forestry, Hainan University, Haikou, China.

The relationship between and its soil microbial community under heat stress was not clear. In this study, the effects of heat stress on the physiological characteristics, soil physicochemical properties and soil microbial community structure of were investigated. The experimental control (CK) was set as day/night (14/10 h) 25/20°C and experimental treatments were set as light heat stress (LHS) 35/30°C and high heat stress (HHS) 40/35°C. Our results showed that, compared with CK, LHS treatment significantly increased malondialdehyde, hydrogen peroxide, proline and soluble sugar contents, as well as catalase and peroxidase activities, while HHS treatment significantly increased ascorbate peroxidase activity and decreased chlorophyll content. Compared with CK, LHS treatment significantly reduced soil ammonium-nitrogen and nitrate-nitrogen content, while HHS significantly increased soil ammonium-nitrogen content. Compared with CK, both treatments changed the soil microbial community structure. For bacterial community, LHS and HHS treatment resulting in the significant enrichment of and , respectively. For fungal community, LHS treatment resulting in the significant enrichment of and . The redundancy analysis showed that plant physiological characteristics, soil ammonium-nitrogen content were significantly correlated with the soil microbial community. Therefore, heat stress altered the soil microbial community structure, and affected the availability of soil available nitrogen, which in turn affected the physiological characteristics of . We suggest that soil microbial community may play an important role in plant resistance to heat stress, and its mechanism deserves further study.
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http://dx.doi.org/10.3389/fpls.2022.950947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355081PMC
July 2022

Greater bone marrow fat and myosteatosis are associated with lower vBMD but not asymptomatic vertebral fracture.

Eur Radiol 2022 Aug 6. Epub 2022 Aug 6.

Department of Radiology, Beijing Jishuitan Hospital, Beijing, China.

Objectives: Organ fat may affect bone metabolism and be associated with vertebral fracture (VF). This study aimed to explore relationships between VF, adiposity indexes measured by MRI, and volumetric BMD (vBMD) measured by quantitative CT (QCT).

Methods: Four hundred volunteers, ranging in age from 22 to 83 years, were recruited and underwent same-day abdominal QCT and chemical shift-encoded (CSE) MRI. We used MRI to quantify the fat content of bone marrow (BMF), psoas major and paraspinal muscles, and the liver. Abdominal fat, VF, and vBMD of the lumbar spine were measured by QCT. For VF discrimination analysis, we examined both the whole cohort (60 VF cases in 30 men and 30 women) and a restricted subgroup of those aged over 50 years (50 VF cases in 23 men and 27 women).

Results: Amongst the men, a 1 SD increase in BMF was associated with a 27.67 (95% CI, -32.71 to -22.62) mg/cm decrease in vBMD after adjusting for age and BMI. Amongst women, all adiposity indexes except for liver fat were significantly associated with vBMD, with BMF having the strongest association (β, -24.00; 95% CI, -28.54 to -19.46 mg/cm). Similar findings were also observed in participants aged over 50 years. The associations of adiposity indexes with vertebral fracture were not significant after adjusting for age in both sexes aged over 50 years.

Conclusions: In both sexes, higher bone marrow fat was associated with lower vBMD at the spine. However, marrow fat and other adipose tissues were not associated with radiographic-based prevalent vertebral fractures.

Key Points: • In both sexes, higher bone marrow fat was associated with lower vBMD at the spine. • Among women, all adiposity indexes except for liver fat content were significantly associated with vBMD, with bone marrow fat having the strongest association. • Marrow fat and other adipose tissues were not associated with radiographic-based asymptomatic vertebral fractures.
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http://dx.doi.org/10.1007/s00330-022-08979-xDOI Listing
August 2022

Value of the prognostic nutritional index (PNI) in patients with newly diagnosed, CD5-positive diffuse large B-cell lymphoma: A multicenter retrospective study of the Huaihai Lymphoma Working Group.

Cancer 2022 Aug 6. Epub 2022 Aug 6.

Department of Hematology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China.

Background: CD5-positive diffuse large B-cell lymphoma (DLBCL) is a clinically rare subtype of DLBCL with aggressive clinical manifestations and a poor prognosis. It has been demonstrated that the prognostic nutritional index (PNI), an indicator of nutritional status and systemic inflammation, is a significant prognostic factor for several types of lymphoma. The objective of this multicenter retrospective study was to explore the prognostic value of the PNI in patients with CD5-positive DLBCL.

Methods: In total, 207 patients with CD5-positive DLBCL were recruited from 11 centers of the Huaihai Lymphoma Working Group. Maximally selected rank statistics analysis was used to identify optimal cutoff points for the PNI. A Cox proportional hazards model was used for univariable and multivariable analyses. Kaplan-Meier curves were used to calculate survival rates and draw survival curves, and the log-rank test was used to compare differences between groups.

Results: The median age at diagnosis was 61 years, and the 5-year overall survival rate was 47.5%. According to the maximally selected rank statistics analysis, a score of 49.7 was the optimal cutoff point for the PNI. Subgroup analysis showed that the PNI could re-stratify patients in BCL-2-negative, MYC-negative, high-intermediate-risk and high-risk International Prognostic Index, BCL-6-positive and BCL-6-negative, high Ki-67 score (≥0.9), Ann Arbor stage III/IV, Eastern Cooperative Oncology Group performance status ≥2, and germinal center B subgroups. Multivariable analysis revealed that PNI, age, Eastern Cooperative Oncology Group performance status, albumin level, and red blood cell count were independent prognostic factors for CD5-positive DLBCL.

Conclusions: The PNI was a significant prognostic indicator for CD5-positive DLBCL and was able to re-stratify the prognosis for clinicopathologic subgroups of patients with CD5-positive DLBCL.
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http://dx.doi.org/10.1002/cncr.34405DOI Listing
August 2022

Metagenomic insights into microbial nitrogen metabolism in two-stage anoxic/oxic-moving bed biofilm reactor system with multiple chambers for municipal wastewater treatment.

Bioresour Technol 2022 Aug 2;361:127729. Epub 2022 Aug 2.

School of Environmental and Municipal Engineering, Qingdao University of Technology, Jialingjiang Road 777, Qingdao 266520, China.

To explore the microbial nitrogen metabolism of a two-stage anoxic/oxic (A/O)-moving bed biofilm reactor (MBBR), biofilms of the system's chambers were analyzed using metagenomic sequencing. Significant differences in microbial populations were found among the pre-anoxic, oxic and post-anoxic MBBRs (P < 0.01). Nitrospira and Nitrosomonas had positive correlations with ammonia nitrogen (NH-N) removal, and were also predominant in oxic MBBRs. These organisms were the hosts of functional genes for nitrification. The denitrifying genera were predominant in anoxic MBBRs, including Thiobacillus and Sulfurisoma in pre-anoxic MBBRs and Dechloromonas and Thauera in post-anoxic MBBRs. The four genera had positive correlations with total nitrate and nitrite nitrogen (NO-N) removal and were the hosts of functional genes for denitrification. Specific functional biofilms with different microbial nitrogen metabolisms were formed in each chamber of this system. This work provides a microbial theoretical support for the two-stage A/O-MBBR system.
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http://dx.doi.org/10.1016/j.biortech.2022.127729DOI Listing
August 2022

Lead induced thymic immunosuppression in Japanese quail (Coturnix japonica) via oxidative stress-based T cell receptor pathway signaling inhibition.

J Inorg Biochem 2022 Jul 29;235:111950. Epub 2022 Jul 29.

College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China. Electronic address:

The human-induced lead (Pb) contamination brings about serious environmental issues around the world and it also poses severe risks to wildlife including birds. Avian thymus is one of primary lymphoid organs and it plays an important role in regulating T cell-based cellular immunity. Therefore, in this study, we tried to examine toxic effects and potential molecular mechanism of Pb on avian thymus using a biological model species-Japanese quail (Coturnix japonica). One-week-old Japanese quails were exposed to 0, 50, 500 and 1000 ppm Pb concentrations in drinking water for three weeks when thymus reached developmental climax. The results showed body weight, thymus weight and thymic size were reduced significantly by Pb exposure. Meanwhile, histopathological changes including vacuolation, cortex atrophy and nuclear debris were detected in thymic cells of Pb exposure. In addition, ultrastructural alterations such as mitochondrial damage, chromatin condensation, and nuclear destruction were found in the thymus of Pb treatments. The increase of reactive oxygen species (ROS) and Malondialdehyde (MDA) as well as the inhibition of antioxidant system indicated that Pb exposure caused oxidative damages in the thymus. Pb exposure also increased thymic cell apoptosis. Moreover, RNA-Seq analysis revealed that thymic functional pathways were disrupted by Pb exposure. Especially, Pb exposure disturbed T cell differentiation and led to T helper type 1 (Th1) /T helper type 2 (Th2) imbalance by interfering with T cell receptor signaling and cytokine signaling. This study implied that Pb caused thymic immunosuppression through causing morphological deformation, structural destruction, oxidation and molecular signaling disruption.
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http://dx.doi.org/10.1016/j.jinorgbio.2022.111950DOI Listing
July 2022

Plasma biomarkers for systemic inflammation in COVID-19 survivors.

Proteomics Clin Appl 2022 Aug 5:e2200031. Epub 2022 Aug 5.

Center of Excellence for Inflammation, Infectious Disease and Immunity, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee, 37614, USA.

Background: While the majority of COVID-19 patients fully recover from the infection and become asymptomatic, a significant proportion of COVID-19 survivors experience a broad spectrum of symptoms lasting weeks to months post-infection, a phenomenon termed "post-acute sequelae of COVID-19 (PASC)". The aim of this study is to determine whether inflammatory proteins are dysregulated and can serve as potential biomarkers for systemic inflammation in COVID-19 survivors.

Methods: We determined the levels of inflammatory proteins in plasma from 22 COVID-19 long haulers (COV-LH), 22 COVID-19 asymptomatic survivors (COV-AS), and 22 healthy subjects (HS) using an Olink proteomics assay and assessed the results by a beads-based multiplex immunoassay.

Results: Compared to HS, we found that COVID-19 survivors still exhibited systemic inflammation, as evidenced by significant changes in the levels of multiple inflammatory proteins in plasma from both COV-LH and COV-AS. CXCL10 was the only protein that significantly upregulated in COV-LH compared with COV-AS and HS.

Conclusions: Our results indicate that several inflammatory proteins remain aberrantly dysregulated in COVID-19 survivors and CXCL10 might serve as a potential biomarker to typify COV-LH. Further characterization of these signature inflammatory molecules might improve the understanding of the long-term impacts of COVID-19 and provide new targets for the diagnosis and treatment of COVID-19 survivors with PASC. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/prca.202200031DOI Listing
August 2022

Cell-Free DNA Sequencing of Intraocular Fluid as Liquid Biopsy in the Diagnosis of Vitreoretinal Lymphoma.

Front Oncol 2022 19;12:932674. Epub 2022 Jul 19.

Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Fudan University, Shanghai, China.

Purpose: To seek novel diagnostic approaches, we improved the workflow of cell-free DNA (cfDNA) sequencing and evaluated its feasibility in vitreoretinal lymphoma (VRL) specimens; the profile of mutations was preliminarily analyzed for potential diagnostic value.

Methods: The study was a diagnostic trial. 23 eyes of 23 patients with VRL and 25 eyes of 25 patients with inflammatory eye diseases were enrolled. Approximate 500μl undiluted vitreous humor and 10ml diluted vitreous fluid was obtained through diagnostic vitrectomy and sent for cytopathological examinations. 500μl of the diluted vitreous fluid was spared for cfDNA sequencing. For cfDNA sequencing, DNA fragmentation procedure was added to the workflow to improve the extraction efficiency; mutations detected were analyzed for potential diagnostic model. The sensitivity and specificity of the cytopathology and cfDNA sequencing were compared. The clinical manifestations were preliminarily analyzed for potential correlations with the genotypes.

Results: CfDNA sequencing was accomplished in 23 eyes with VRL and 20 eyes with inflammatory eye diseases. VRL-related mutated genes included (18 eyes, 78%), (11 eyes, 48%), (11 eyes,48%), (7 eyes, 30%), (7 eyes, 30%), (6 eyes, 26%), (6 eyes, 26%), etc. Logistic regression based on the mutations of and was of the potential for the diagnosis of VRL (P<0.001, adjusted R2 = 0.789, sensitivity 0.913, specificity 0.950); by comparison, the sensitivity and specificity of the vitreous cytopathology were 0.826 and 1.000, respectively. Further analysis of the mutation profile showed that patients carrying mutation tended to have higher intraocular interleukin-10 level (P=0.030), that CARD11 mutation was correlated with younger age at ocular onset (P=0.039), and that patients with intracranial involvement carried more multiple-site mutations in the gene (P=0.013).

Conclusions: The improved workflow of CfDNA sequencing is of sound feasibility in a limited amount of vitreous humor. The logistic model based on the mutations could help to provide reliable clues for the diagnosis of VRL.
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http://dx.doi.org/10.3389/fonc.2022.932674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343589PMC
July 2022

CD106/VCAM-1 distinguishes a fibroblast subpopulation with high colony-forming capacity and distinct protein expression from the uterosacral ligament.

Ann Transl Med 2022 May;10(9):511

Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.

Background: Pelvic organ prolapse (POP) is a common degenerative disease in women which may diminish quality of life. Investigating the pathological changes of the uterosacral ligament, including the functional changes of fibroblasts, is critical to understanding the pathophysiology of POP. This study was designed to isolate CD106-positive (CD106) fibroblasts from the human uterosacral ligament and assess the function and expression of this subpopulation.

Methods: We separated CD106 fibroblasts and CD106 negative (CD106) fibroblasts by fluorescence-activated cell sorting (FACS) and cultured them for subsequent experiments. Flow cytometric analysis was used to test the sorting efficiency, CD106 expression, and typical mesenchymal stem cell (MSC) phenotype marker expression. A colony-forming unit (CFU) assay was applied to evaluate the colony-forming ability of the fibroblasts. Trilineage differentiation capacities were assessed after induction. The protein levels of vimentin, fibroblast specific protein-1 (FSP-1), collagen I (COL 1), matrix metallopeptidase-1 (MMP-1), and α-smooth muscle actin (α-SMA) were detected by western blot analysis. The expression of CD106 was verified by flow cytometric analysis and immunohistochemistry (IHC) in the POP and non-POP groups.

Results: The CD106 fibroblasts were isolated with a purity of (93.50±3.91)%. The CD106 fibroblasts exhibited higher colony-forming capacity than that of CD106 fibroblasts, but neither of them showed adipogenic or osteogenic differentiation similar to that of MSCs. The protein levels of MMP-1 and α-SMA were lower, and the level of COL 1 was higher in the CD106 fibroblasts than in the CD106- fibroblasts. In addition, we observed a decreased expression of CD106 in the POP group compared with the non-POP group.

Conclusions: Our results suggest that CD106 fibroblasts possess a high colony-forming capacity and distinct protein expression, and this subpopulation is reduced in POP.
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http://dx.doi.org/10.21037/atm-21-5136DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347059PMC
May 2022

Genetic associations with healthy ageing among Chinese adults.

NPJ Aging 2022 May 2;8(1). Epub 2022 May 2.

Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, 138672, Singapore.

The genetic basis of overall healthy ageing, especially among the East-Asian population is understudied. We conducted a genome-wide association study among 1618 Singapore Chinese elderly participants (65 years or older) ascertained to have aged healthily and compared their genome-wide genotypes to 6221 participants who did not age healthily, after a 20-year follow-up. Two genetic variants were identified (P < 2.59 × 10) to be associated with healthy aging, including the LRP1B locus previously associated in long-lived individuals without cognitive decline. Our study sheds additional insights on the genetic basis of healthy ageing.
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http://dx.doi.org/10.1038/s41514-022-00086-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9158790PMC
May 2022

Optically Triggering and Monitoring Single-Cell-Level Metabolism Using Ormosil-Decorated Ultrathin Fibers.

Langmuir 2022 Aug 4. Epub 2022 Aug 4.

Key Laboratory of Photochemistry, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

The integration of biological components and artificial devices requires a bio-machine interface that can simultaneously trigger and monitor the activities in biosystems. Herein, we use an organically modified silicate (ormosil) composite coating containing a light-responsive nanocapsule and a fluorescent bioprobe for reactive oxygen species (ROS) to decorate ultrathin optical fibers, namely, ormosil-decorated ultrathin fibers (OD-UFs), and demonstrate that these OD-UFs can optically trigger and monitor the intracellular metabolism activities in living cells. The sizes and shapes of UF tips were finely controlled to match the dimension and mechanical properties of living cells. The increased elasticity of the ormosil coating of OD-UFs reduces possible mechanical damage during the cell membrane penetration. The light-responsive nanocapsule was physically absorbed on the surface of the ormosil coating and could release a stimulant to trigger the metabolism activities in cells upon the guided laser through OD-UFs. The fluorescent bioprobe was covalently linked with the ormosil matrix for monitoring the intracellular ROS generation, which was verified by the experiments on the microdroplets of a hydrogen peroxide solution. Finally, we found that the living cells could maintain most of their viability after being inserted with OD-UFs, and the intracellular metabolism activities were successfully triggered and monitored at the single-cell level. The OD-UF provides a new platform for the investigation of intracellular behaviors for drug stimulations and represents a new proof of concept for a bio-machine interface based on the optical and chemical activities of organic functional molecules.
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http://dx.doi.org/10.1021/acs.langmuir.2c00915DOI Listing
August 2022

Fine mapping and target gene identification of , a QTL for stigma exsertion rate in rice ( L.).

Front Plant Sci 2022 18;13:959859. Epub 2022 Jul 18.

State Key Laboratory of Rice Biology, Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture, China National Rice Improvement Centre, China National Rice Research Institute, Hangzhou, China.

The stigma exsertion rate (SER) is a complex agronomy phenotype controlled by multiple genes and climate and a key trait affecting the efficiency of hybrid rice seed production. Using a japonica two-line male sterile line (DaS) with a high SER as the donor and a tropical japonica rice (D50) with a low SER as the acceptor to construct a near-isogenic line [NIL ( )]. Populations were segregated into 2,143 individuals of BCF and BCF, and the stigma exsertion quantitative trait locus (QTL) was determined to be located within 410.4 Kb between markers RM17157 and RM17227 on chromosome 4. Bioinformatic analysis revealed 13 candidate genes in this region. Sequencing and haplotype analysis indicated that the promoter region of () had a one-base substitution between the two parents. Further Reverse Transcription-Polymerase Chain Reaction (RT-PCR) analysis showed that the expression level of in DaS was significantly higher than in D50. After knocking out in the DaS background, it was found that the SER of (the total SER of the and the were 62.54 and 66.68%, respectively) was significantly lower than that of the wild type (the total SER was 80.97%). Transcriptome and hormone assay analysis showed that had significantly higher auxin synthesis genes and contents than the wild type and the expression of auxin signaling-related genes was significantly different, Similar results were observed for abscisic acid and jasmonic acid. These results indicate that is mostly likely the target gene of , and the study of its gene function is of great significance for understanding the molecular mechanisms of SER and improving the efficiency of hybrid seed production.
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http://dx.doi.org/10.3389/fpls.2022.959859DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341389PMC
July 2022

VCAM-1-binding peptide targeted cationic liposomes containing NLRP3 siRNA to modulate LDL transcytosis as a novel therapy for experimental atherosclerosis.

Metabolism 2022 Jul 30;135:155274. Epub 2022 Jul 30.

Department of Endocrinology, Institute of Geriatric Medicine, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430077, China; Department of Pharmacology, the Key Laboratory of Drug Target Researches and Pharmacodynamics Evaluation of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address:

Background: Activation of NLRP3 inflammasome accelerates the formation of atherosclerotic plaques. Here, we evaluated the effects of inflammation on the expression of the NLRP3 inflammasome in endothelial cells (ECs).

Methods: The effect of TNF-α on transcytosis of LDL was measured. VCAM-1 binding peptide targeting cationic liposomes (PCLs) were prepared as siRNA vectors. Methylated NLRP3 siRNA was encapsulated into the PCLs to knock down NLRP3 in vitro and in vivo. In rats with partial carotid ligation, TNF-α-induced LDL retention in the carotid artery endothelium was observed. In ApoE mice, NLRP3 siRNA-PCLs were injected intravenously to observe their effect on the formation of atherosclerosis.

Results: Our results showed that TNF-α upregulated NLRP3 in ECs, promoting the assembly of the NLRP3 inflammasome and processing of pro-IL-1β into IL-1β. Moreover, TNF-α accelerated LDL transcytosis in ECs. Knockdown of NLRP3 prevented TNF-α-induced NLPR3 inflammasome/IL-1β signaling and LDL transcytosis. Using optimized cationic liposomes to encapsulate methylated NLRP3 siRNA, resulting in targeting of VCAM-1-expressing ECs, to knockdown NLRP3, TNF-α-induced NLRP3 inflammasome activation and LDL transcytosis were prevented. Using the partial carotid ligation as an atherosclerosis rat model, we found that local administration of NLRP3 siRNA-PCLs efficiently knocked down NLPR3 expression in the carotid endothelium and dramatically attenuated the deposition of atherogenic LDL in carotid ECs in TNF-α-challenged rats. Furthermore, NLRP3 siRNA-PCLs were injected intravenously in ApoE mice, resulting in reduced plaque formation.

Conclusion: These findings established a novel strategy for targeting the NLRP3 inflammasome using NLRP3 siRNA-PCLs to interrupt LDL transcytosis, representing a potential novel therapy for atherosclerosis.
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http://dx.doi.org/10.1016/j.metabol.2022.155274DOI Listing
July 2022

Clinical manifestations of COVID-19: An overview of 102 systematic reviews with evidence mapping.

J Evid Based Med 2022 Jul 31. Epub 2022 Jul 31.

School of Public Health, Lanzhou University, Lanzhou, China.

Objective: Coronavirus disease 2019 (COVID-19) has rapidly spread worldwide, but there is so far no comprehensive analysis of all known symptoms of the disease. Our study aimed to present a comprehensive picture of the clinical symptoms of COVID-19 using an evidence map.

Methods: We systematically searched MEDLINE via PubMed, Web of Science, Embase, and Cochrane library from their inception to March 16, 2021. We included systematic reviews reporting the clinical manifestations of COVID-19 patients. We followed the PRISMA guidelines, and the study selection, data extraction, and quality assessment were done by two individuals independently. We assessed the methodological quality of the studies using AMSTAR. We visually presented the clinical symptoms of COVID-19 and their prevalence.

Results: A total of 102 systematic reviews were included, of which, 68 studies (66.7%) were of high quality, 19 studies (18.6%) of medium quality, and 15 studies (14.7%) of low quality. We identified a total of 74 symptoms including 17 symptoms of the respiratory system, 21 symptoms of the neurological system, 10 symptoms of the gastrointestinal system, 16 cutaneous symptoms, and 10 ocular symptoms. The most common symptoms were fever (67 studies, ranging 16.3%-91.0%, pooled prevalence: 64.6%, 95%CI, 61.3%-67.9%), cough (68 studies, ranging 30.0%-72.2%, pooled prevalence: 53.6%, 95%CI, 52.1%-55.1%), muscle soreness (56 studies, ranging 3.0%-44.0%, pooled prevalence: 18.7%, 95%CI, 16.3%-21.3%), and fatigue (52 studies, ranging 3.3%-58.5%, pooled prevalence: 29.4%, 95%CI, 27.5%-31.3%). The prevalence estimates for COVID-19 symptoms were generally lower in neonates, children and adolescents, and pregnant women than in the general populations.

Conclusion: At least 74 different clinical manifestations are associated with COVID-19. Fever, cough, muscle soreness, and fatigue are the most common, but attention should also be paid to the rare symptoms that can help in the early diagnosis of the disease.
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http://dx.doi.org/10.1111/jebm.12483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353366PMC
July 2022

Classifications of Qualitative Characteristics on Angle Configurations via Ultrasound Biomicroscopy in Acute Primary Angle Closure.

Clin Interv Aging 2022 21;17:1113-1125. Epub 2022 Jul 21.

Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, People's Republic of China.

Purpose: To study the classifications of qualitative characteristics on the angle configurations in the acute primary angle closure (APAC) and fellow eyes by ultrasound biomicroscopy (UBM).

Methods: A total of 131 patients (262 eyes) were researched retrospectively. The qualitative parameters from UBM images were classified into iris form (IF), ciliary body configuration (CBC), basal iris thickness (BIT), iris convexity (IC), iris insert (II), iris angulation (IA), ciliary body size (CBS) and ciliary body position (CBP). Comparative analyses between the APAC (case group) and fellow (control group) eyes were performed.

Results: There were significant differences in IF, CBC, IC, II, CBS, CBP between the case group and control group in all quadrants (<0.001). The IA of the case group and control group presented significant difference in all quadrants (=0.001). However, there was not a significant difference in BIT between the case group and control group in all quadrants (=0.495). The case group had fewer parallelogram-like and mushroom-like and more cone-like and hook-like CBCs than the control group (<0.001).

Conclusion: Multiple ciliary body configurations can influence the stability of the lens and the anatomic configuration of the anterior chamber angle indirectly. New qualitative classification system of UBM may be more intuitionistic and refined to reflect the angle configurations to help clinical practice.
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http://dx.doi.org/10.2147/CIA.S367186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315054PMC
August 2022

Correction: Transcription factor EB-mediated mesenchymal stem cell therapy induces autophagy and alleviates spinocerebellar ataxia type 3 defects in neuronal cells model.

Cell Death Dis 2022 Jul 28;13(7):654. Epub 2022 Jul 28.

CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.

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http://dx.doi.org/10.1038/s41419-022-05119-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334382PMC
July 2022

Chronic effects of benzalkonium chlorides on short chain fatty acids and methane production in semi-continuous anaerobic digestion of waste activated sludge.

Sci Total Environ 2022 Jul 25;847:157619. Epub 2022 Jul 25.

School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212100, China.

As an emerging pollutant, benzalkonium chlorides (BACs) potentially enriched in waste activated sludge (WAS). However, the microbial response mechanism under chronic effects of BACs on acidogenesis and methanogenesis in anaerobic digestion (AD) has not been clearly disclosed. This study investigated the AD (by-)products and microbial evolution under low to high BACs concentrations from bioreactor startup to steady running. It was found that BACs can lead to an increase of WAS hydrolysis and fermentation, but a disturbance to acidogenic bacteria also occurred at low BACs concentration. A noticeable inhibition to methanogenesis occurred when BAC concentration was up to 15 mg/g TSS. Metagenomic analysis revealed the key genes involved in acetic acid (HAc) biosynthesis (i.e. phosphate acetyltransferase, PTA), β-oxidation pathway (acetyl-CoA C-acetyltransferase) and propionic acid (HPr) conversion was slightly promoted compared with control. Furthermore, BACs inhibited the acetotrophic methanogenesis (i.e. acetyl-CoA synthetase), especially BAC concentration was up to 15 mg/g TSS, thereby enhanced short chain fatty acids (SCFAs) accumulation. Overall, chronic stimulation of functional microorganisms with increasing concentrations of BACs impact WAS fermentation.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157619DOI Listing
July 2022

Neural Dynamics Underlying Cognitive Control Modulated by Reinforcement Learning of Irrelevant Stimulus-Response Associations.

J Cogn Neurosci 2022 Jul 22:1-17. Epub 2022 Jul 22.

South China Normal University.

Human behavioral studies have shown that reward has multifaceted (motivational, learning, and emotional) effects on cognitive control. Past studies have extensively explored the neural underpinning of the motivational effect of reward on cognitive control, but less is known on the other components. The present study investigates the learning effect of reward on cognitive control with a novel paradigm reinforcing task-irrelevant stimulus-response (S-R) associations. We manipulated the contingency of irrelevant spatial stimulus-response-reward between participants (n = 54) in an orthogonal Simon task while EEG recording. The behavioral results showed that the orthogonal Simon effect in RTs and error rates increased or reversed when performance-contingent reward always followed spatially compatible or incompatible trials. The EEG results showed that the initial incorrect activation of the lateralized readiness potential, reflecting response preparation based on irrelevant spatial S-R associations, was present in the incompatible or compatible condition when reward always followed compatible or incompatible trials. Meanwhile, the P3b amplitude also showed a similar pattern of increase or reversal depending on the contingency of irrelevant stimulus-response-reward. These findings suggest that the brain used reward-strengthened irrelevant spatially compatible or incompatible S-R associations to predict responses. Moreover, the frontocentral N2 amplitude and theta band (4-8 Hz) power showed similar reward-related increase or reversal in the conflict effect, suggesting that there was conflict when the response predicted by the reward-strengthened irrelevant S-R associations was different from the correct response. Taken together, these results suggest that reinforcement learning of irrelevant spatial S-R associations can modulate cognitive control.
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http://dx.doi.org/10.1162/jocn_a_01895DOI Listing
July 2022

Thermal stress involved in TRPV2 promotes tumorigenesis through the pathways of HSP70/27 and PI3K/Akt/mTOR in esophageal squamous cell carcinoma.

Br J Cancer 2022 Jul 27. Epub 2022 Jul 27.

CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.

Background: The transient receptor potential vanilloid receptor 2 (TRPV2) has been found to participate in the pathogenesis of various types of cancers, however, its role(s) in the tumorigenesis of ESCC remain poorly understood.

Methods: Western blotting and immunohistochemistry were performed to determine the expression profiles of TRPV2 in the ESCC patient tissues. A series of in vitro and in vivo experiments were conducted to reveal the role of TRPV2 in the tumorigenesis of ESCC.

Results: Our study first uncovered that the activation of TRPV2 by recurrent acute thermal stress (54 °C) or O1821 (20 μM) promoted cancerous behaviours in ESCC cells. The pro-angiogenic capacity of the ESCC cells was found to be enhanced profoundly and both tumour formation and metastasis that originated from the cells were substantially promoted in nude mouse models upon the activation of TRPV2. These effects were inhibited significantly by tranilast (120 μM) and abolished by TRPV2 knockout. Conversely, overexpression of TRPV2 could switch the cells to tumorigenesis upon activation of TRPV2. Mechanistically, the driving role of TRPV2 in the progression of ESCC is mainly regulated by the HSP70/27 and PI3K/Akt/mTOR signalling pathways.

Conclusions: We revealed that TRPV2-PI3K/Akt/mTOR is a novel and promising target for the prevention and treatment of ESCC.
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http://dx.doi.org/10.1038/s41416-022-01896-2DOI Listing
July 2022

Breast cancer-related lymphoedema and resistance exercise: An evidence-based review of guidelines, consensus statements and systematic reviews.

J Clin Nurs 2022 Jul 27. Epub 2022 Jul 27.

School of Nursing, Peking University, Beijing, China.

Aims And Objectives: Breast cancer-related lymphoedema (BCRL) is a side effect of cancer treatment and can be alleviated by resistance exercise. This systematic, evidence-based review examined the existing best evidence on resistance exercise for BCRL to accurately describe the current status of the field and offer recommendations for clinicians.

Methods: This review adheres to the PRISMA guidelines. Clinical practice guidelines, consensus documents, systematic reviews and other related evidence-based resources about resistance exercise for BCRL were retrieved through the English databases and guideline websites. The publication data limit was set to December 2020. The following search terms were used: 'breast cancer/breast neoplasm/breast carcinoma/breast tumor/breast malignancy, lymphedema/swelling/edema/lymphoedema, resistance/weight/strength training, best practice/clinical practice/guideline/consensus documents'. The quality of the included studies was evaluated by two authors independently using AGREE II and AMSTAR II tools. Evidence-based recommendations on resistance exercise relevant for BCRL were synthesised and categorised.

Results: Twenty two articles (seven guidelines, four consensus documents and eleven systematic reviews) were included. The overall quality of the eleven eligible guidelines and consensus documents was moderate to high according to the AGREE II criteria. The quality of the eleven systematic reviews was critically low to high according to the AMSTAR criteria. Six clinical topics involving 43 recommendations were identified. Recommendations were categorised by safety of resistance training, effectiveness of resistance training, evaluation prior to resistance exercise, resistance exercise prescription, resistance training outcome index and points for attention.

Conclusions: This study summarises 43 recommendations for resistance training for BCRL and provides guidance for clinicians. Based on randomised trials and systematic reviews published in recent years, there is an urgent need to update the guidelines and consensus documents in terms of topics, for example effectiveness of resistance training and resistance training outcome index.
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http://dx.doi.org/10.1111/jocn.16437DOI Listing
July 2022

Study on Static and Fatigue Behaviors of Steel-UHPFRC Composite Deck Structure.

Polymers (Basel) 2022 Jul 8;14(14). Epub 2022 Jul 8.

Yellow River Engineering Consulting Co., Ltd., Zhengzhou 450003, China.

Ultra-high-performance fiber-reinforced cementitious composite (UHPFRC) is used in orthotropic steel deck (OSD) to form a lightweight composite deck structure (LWCD), which is expected to solve the problems of fatigue cracking of traditional steel deck and pavement damage. This paper aims to study the influence of key design parameters on longitudinal bending and transverse fatigue performance, as well as the ultimate bearing capacity calculation theory of the LWCD. A local finite-element (FE) model was built to evaluate the vehicle-induced stress ranges of six typical fatigue-prone details. In total, eight negative bending tests on steel-UHPFRC composite beams and one fatigue test on a steel-UHPFRC composite plate were conducted to investigate the longitudinal bending performance and the transverse flexural fatigue behavior of the LWCD, respectively. The results show that adding a 60-mm UHPFRC layer can significantly reduce the stress amplitude of six typical fatigue details by 44.8% to 90%. The failure mode of the longitudinal bending tests is the U-rib buckle and all UHPFRC layers exhibit multiple cracking behaviors when the specimens failed. The longitudinal cracking stresses of the specimens are between 20.0 MPa to 27.3 MPa. The reinforcement ratio and cover thickness have a great influence on the cracking stress. While the ultimate bearing capacity of specimens with different parameters has little difference. The calculation method of the ultimate bearing capacity of a steel-UHPFRC composite structure is proposed. When the strain at the bottom of the u-rib is taken as 1.2 times the design yield strain, the calculated results are in good agreement with the experimental results. No fatigue failure was observed after 66.12 million fatigue cycles under the design load, highlighting the favorable fatigue resistance of the proposed LWCD.
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http://dx.doi.org/10.3390/polym14142796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317259PMC
July 2022

Identification of Exported Proteins That Bind to the Erythrocyte Cytoskeleton.

Microorganisms 2022 Jul 16;10(7). Epub 2022 Jul 16.

Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA.

proteins are exported to the erythrocyte cytoplasm to create an environment that supports parasite replication. Although hundreds of proteins are predicted to be exported through export element (PEXEL)-dependent and -independent mechanisms, the functions of exported proteins are largely uncharacterized. In this study, we used a biochemical screening approach to identify putative exported proteins that bound to inside-out vesicles prepared from erythrocytes. Out of 69 PEXEL-motif proteins tested, 18 bound to inside-out vesicles (IOVs) in two or more independent assays. Using co-affinity purifications followed by mass spectrometry, pairwise co-purification experiments, and the split-luciferase assay, we identified 31 putative protein-protein interactions between erythrocyte cytoskeletal proteins and predicted exported proteins. We further showed that PF3D7_1401600 binds to the spectrin-binding domain of erythrocyte ankyrin via its MESA erythrocyte cytoskeleton binding (MEC) motif and to the N-terminal domains of ankyrin and 4.1R through a fragment that required an intact helical interspersed sub-telomeric (PHIST) domain. Introduction of PF3D7_1401600 into erythrocyte ghosts increased retention in the microsphiltration assay, consistent with previous data that reported a reduction of rigidity in red blood cells infected with -deficient parasites.
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http://dx.doi.org/10.3390/microorganisms10071438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320996PMC
July 2022

Investigation on the Air Permeability and Pore Structure of Concrete Subjected to Carbonation under Compressive Stress.

Materials (Basel) 2022 Jul 7;15(14). Epub 2022 Jul 7.

State Key Laboratory of Green Building Materials, China Building Materials Academy, Beijing 100024, China.

Concrete structures have to withstand the combined effects of external load and environmental factors. Therefore, it is meaningful to study the durability of concrete under compression and carbonation. The air permeability coefficient () and pore structure of concrete under uniaxial compression and carbonation were measured by the Autoclam method and mercury intrusion porosimetry (MIP). The Autoclam test results showed that the concrete changed in a concave parabolic manner with the compressive stress level, and the inflection point of the stress level was 45%. The MIP results showed that the characteristic pore structural parameters (porosity, average pore diameter, median pore diameter by area, and median pore diameter by volume) first decreased and then increased with the stress level change. The change in concrete microstructure was a result of the combined effect of pore filling, decalcification, and densification, as well as the split effect. The key pore structural parameters affecting were confirmed using gray relational analysis (GRA). The top three parameters with the highest correlation with the carbonated concrete were porosity (gray relational grade = 0.789), median pore diameter by volume ( = 0.763), and proportion of transition pore volume ( = 0.827). Furthermore, the regression analysis showed a good linear relation between and the important pore structural parameters.
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http://dx.doi.org/10.3390/ma15144775DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321981PMC
July 2022

Puerarin alleviates LPS-induced H9C2 cell injury by inducing mitochondrial autophagy.

J Cardiovasc Pharmacol 2022 Jul 11. Epub 2022 Jul 11.

Department of Cardiology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.

Abstract: Sepsis leads to the damage of multiple organs, and thereby adversely affects the cardiovascular system. At present, no effective method has been found to treat myocardial injury caused by sepsis. While Puerarin was reported to attenuate LPS-induced mitochondrial injury in HUVECs, the effects of Puerarin in sepsis-induced myocardial injury remain unclear. In this study, H9C2 cells were stimulated with LPS, CCK-8 assays were performed to assess cell viability, and flow cytometry and TUNEL staining were used to assess cell apoptosis. Levels of ATP, ADP, AMP and enzyme activity were investigated using commercial kits. ROS levels in H9C2 cells were detected by flow cytometry. Autophagosomes in the mitochondria of H9C2 cells were observed by transmission electron microscope (TEM), and protein expression was assessed by western blotting. We found that Puerarin significantly reversed LPS-induced decreases in H9C2 cell viability by inhibiting apoptosis. The ROS levels in H9C2 cells were significantly upregulated by LPS, but that effect was markedly reduced by Puerarin. In addition, Puerarin attenuated LPS-induced mitochondrial injury in H9C2 cells by regulating dynamin-related protein 1 (Drp1) and mitofusin 1 (Mfn1). LPS decreased enzyme activity and reduced the levels of ADP, ALP, and AMP in mitochondria; however, those effects were reversed by Puerarin. Puerarin and Torin1 reversed LPS-induced inhibition of autophagy in the mitochondria of H9C2 cells via mediation of p62, LC3B, Pink1, and Parkin. Puerarin inhibited LPS-induced H9C2 cell injury by inducing mitochondrial autophagy, which acts as a mechanism for preventing myocardial injury caused by sepsis.
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http://dx.doi.org/10.1097/FJC.0000000000001315DOI Listing
July 2022

Genome-Wide Association Study of Partial Resistance to in Wild Soybeans from Heilongjiang Province, China.

Curr Issues Mol Biol 2022 Jul 17;44(7):3194-3207. Epub 2022 Jul 17.

Crop Tillage and Cultivation Institute of Heilongjiang Academy of Agricultural Sciences (HAAS), Harbin 150086, China.

Phytophthora root rot (PRR) is a destructive disease of soybeans ( (L.) Merr) caused by (). The most effective way to prevent the disease is growing resistant or tolerant varieties. Partial resistance provides a more durable resistance against the pathogen compared to complete resistance. Wild soybean ( Sieb. & Zucc.) seems to be an extraordinarily important gene pool for soybean improvement due to its high level of genetic variation. In this study, 242 wild soybean germplasms originating from different regions of Heilongjiang province were used to identify resistance genes to race 1 using a genome-wide association study (GWAS). A total of nine significant SNPs were detected, repeatedly associated with resistance and located on chromosomes 1, 10, 12, 15, 17, 19 and 20. Among them, seven favorable allelic variations associated with resistance were evaluated by a t-test. Eight candidate genes were predicted to explore the mechanistic hypotheses of partial resistance, including , which encodes an LRR receptor-like serine/threonine protein kinase protein, , which encodes a receptor-like cytosolic serine/threonine protein kinase protein. These findings will provide additional insights into the genetic architecture of resistance in a large sample of wild soybeans and -resistant breeding through marker-assisted selection.
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http://dx.doi.org/10.3390/cimb44070221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319971PMC
July 2022
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