Publications by authors named "Ling Cheng"

225 Publications

Acupuncture for drooling in children with cerebral palsy: A protocol for systematic review.

Medicine (Baltimore) 2021 Apr;100(14):e25393

Jiangxi University of Traditional Chinese Medicine, Nanchang, PR China.

Background: The aim of this study is to provide the methods used to evaluate the effectiveness and safety of acupuncture therapy for treating drooling in children with cerebral palsy.

Methods And Analysis: A comprehensive search of Pubmed, Embase, Cochrane Central Register of Controlled Trials, Web of Science, 4 Chinese databases (China National Knowledge Infrastructure, Chinese Biomedical Literatures database, Wan-Fang Database and Chinese Science and Technology Periodicals will be conducted to identify randomized controlled trials of acupuncture for treating children with cerebral palsy salivation with no restriction on time or language. The primary outcome of this systematic review will be the effective rate. The risk of bias will be implemented according to Cochrane Handbook for Systematic Reviews of Interventions. We will conduct the meta-analysis to synthesize the evidence for each outcome, if possible. The heterogeneity will be evaluated statistically using the χ2 test and the I2 statistic. The random-effect model will be used to provide more conservative results, if significant heterogeneity is identified (I2 > 50% or P < .10).

Ethics/dissemination: Our findings will be disseminated in a peer-reviewed journal and at conference meetings. It is not necessary for formal ethical approval as no primary data are collected.

Trial Registration Number: INPLASY2020110024.
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http://dx.doi.org/10.1097/MD.0000000000025393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036064PMC
April 2021

Cnngeno: A high-precision deep learning based strategy for the calling of structural variation genotype.

Comput Biol Chem 2020 Nov 21:107417. Epub 2020 Nov 21.

Department of Computer Science and Technology, Beijing University of Chemical Technology, Beijing, China. Electronic address:

Genotype plays a significant role in determining characteristics in an organism and genotype calling has been greatly accelerated by sequencing technologies. Furthermore, most parametric statistical models are unable to effectively call genotype, which is influenced by the size of structural variations and the coverage fluctuations of sequencing data. In this study, we propose a new method for calling deletions' genotypes from the next-generation data, called Cnngeno. Cnngeno can convert sequencing data into images and classifies the genotypes from these images using the convolutional neural network(CNN). Moreover, Cnngeno adopted the convolutional bootstrapping strategy to improve the anti-noisy label's ability. The results show that Cnngeno performs better in terms of precision for calling genotype when compared with other existing methods. The Cnngeno is an open-source method, available at https://github.com/BRF123/Cnngeno.
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http://dx.doi.org/10.1016/j.compbiolchem.2020.107417DOI Listing
November 2020

N and O multi-coordinated vanadium single atom with enhanced oxygen reduction activity.

J Colloid Interface Sci 2021 Mar 18;594:466-473. Epub 2021 Mar 18.

Department of Materials Science & Engineering, University of Science and Technology of China, Jinzhai Road NO.96, Hefei, Anhui 230026, PR China; The Anhui High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, PR China. Electronic address:

Recently, atomically dispersed transition-metal single atom in nitrogen-doped carbon matrix as electrocatalysts has aroused general interest. However, there is no report about vanadium single atom for ORR in the literature. According to d-band center theory for transition-metals, the performance of catalysts is regulated by the electronic structure of the catalytic center which determines the intermediate adsorption kinetics. Indeed, the valence of vanadium is variable, its electron structure could be modulated by an appropriate coordination structure. Here, a novel method is developed to prepare the N and O co-coordinated vanadium single atom (V-NO) embedded in the carbon matrix. The catalyst displays a half-wave potential of 865 mV in base solution which surpasses 20% Pt/C, and also shows a high power density of 180 mW/cm in Zn-air batteries. DFT calculations reveal that the N and O coordination configuration could regulate the electron structure and geometry of vanadium to boost the electrocatalytic activity.
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http://dx.doi.org/10.1016/j.jcis.2021.03.074DOI Listing
March 2021

School-based interventions for the treatment of childhood obesity: a systematic review, meta-analysis and meta-regression of cluster randomised controlled trials.

Public Health Nutr 2021 Mar 22:1-13. Epub 2021 Mar 22.

Alice Lee Centre for Nursing Studies, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.

Objective: Schools offer an ideal setting for childhood obesity interventions due to their access to children and adolescents. This review aimed to systematically review the impact of school-based intervention for the treatment of childhood obesity.

Design: Eight databases were searched from inception till 30 May 2020. A revised Cochrane risk-of-bias tool and the Grading of Recommendations, Assessment, Development and Evaluations criteria were used to evaluate the risk of bias and overall evidence. Meta-analysis and meta-regression were performed on Stata software using the random effects model. Overall effect was evaluated using Hedges' g, and heterogeneity was assessed using Cochran's Q and I2.

Setting: Cluster randomised controlled trials (cluster-RCT) delivered in school.

Participants: Children and adolescents (6-18 years of age) with overweight and obesity.

Results: Twelve cluster-RCT from seven countries with 1755 participants were included in the meta-analysis. School-based interventions for the treatment of childhood obesity reduced BMI and BMI z-scores with a medium effect (g = 0·52). Subgroup analyses showed greater effectiveness of brief school-based interventions and the interventions conducted in lower-middle to upper-middle economies. Meta-regression assessed the heterogeneity and the final model, with covariates of the type of economies and trial duration, accounted for 41·2 % of the variability. The overall quality of evidence was rated low because of the high risk of bias and inconsistency.

Conclusions: School-based interventions are a possible approach to provide universal healthcare for the treatment of childhood obesity, and further well-designed cluster-RCT with longer follow-up are needed. This study is registered with PROSPERO (CRD42020160735).
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http://dx.doi.org/10.1017/S1368980021001117DOI Listing
March 2021

Fritchman modeling for correlated turbulence-induced errors in FSO communication.

Appl Opt 2021 Mar;60(7):1801-1808

Free-space-optics-based communication links are an attractive potential solution for solving the last mile challenge but suffer from turbulence-induced fading. This fading causes errors in the received signal. While models exist to predict how turbulence affects the signal, these models often do not account for the channel's memory. Typical fading models accurately predict the average effect of the channel on a signal but not the distribution of the errors and the individual lengths of events such as deep fades. To better model the channel, this paper presents an alternative approach: Fritchman Markov modeling. The models produced accurately match the behavior of the channel and can be used to develop robust and efficient error mitigation strategies in the future.
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http://dx.doi.org/10.1364/AO.414175DOI Listing
March 2021

Photoelectrocatalytic degradation of deoxynivalenol on CuO-CuO/WO ternary film: Mechanism and reaction pathways.

Sci Total Environ 2021 Feb 13;776:145840. Epub 2021 Feb 13.

Key Laboratory of Material Chemistry for Energy Conversion and Storage (Ministry of Education), School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address:

A ternary film of CuO-CuO/WO possessing high visible-light photoelectrocatalytic (PEC) performance was prepared for degradation of deoxynivalenol (DON). In such a ternary film, the introduction of CuO-CuO significantly promoted the absorption of WO in the visible region and reduced the recombination of photogenerated charge carriers. As a result, the CuO-CuO/WO film exhibited high photoelectrochemical activity under visible light illumination. The PEC performance of CuO-CuO/WO film was evaluated by the decoloration of Rhodamine B. The result indicated that the CuO-CuO/WO film exhibited higher PEC activity than WO or CuO-CuO film. When the CuO-CuO/WO film was applied to study the removal of DON, the degradation efficiency reached 87.6% after 180-min PEC treatment. According to reactive oxygen species detected by electron spin resonance analysis, a Z-scheme and type-II PEC mechanism was proposed for this ternary film. Furthermore, the intermediates formed during the PEC degradation process of DON were separated by high-performance liquid chromatography and identified with liquid chromatography-mass spectrometry. On the basis of sixteen intermediate products identified, we proposed a degradation pathway for DON in such a PEC system.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145840DOI Listing
February 2021

Government Intervention Measures Effectively Control COVID-19 Epidemic in Wuhan, China.

Curr Med Sci 2021 Feb 13;41(1):77-83. Epub 2021 Feb 13.

Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

The Coronavirus disease 2019 (COVID-19) outbreak has been brought under control through a nationwide effort, and now it has become a global pandemic and the situation seems grim. We summarized the measures taken in Wuhan and analyzed the effects to comprehensively describe the factors involved in controlling the COVID-19 in China. In China, several measures such as the lockdown of Wuhan, restriction of traffic and communities, increasing hospital beds, nationwide support from medical staff, epidemic prevention equipment and supplies, and establishment of makeshift shelter hospitals have been taken. The lockdown of Wuhan reduced the propagation of cases to other cities in Hubei province and throughout China, traffic and community restrictions reduced the flow of population and the spread of disease, increasing wards and beds and medical personnel reduced the incidence of severe cases and mortality, the establishment of the Fangcang shelter hospitals provided a good isolation and monitoring environment, and further reduced the spread and fatality of the disease. The fact that China was able to control the spread of COVID-19 within three months without a specific drug or vaccine suggests that these measures are more adequate and effective.
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http://dx.doi.org/10.1007/s11596-021-2321-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881908PMC
February 2021

Hypermethylation of Mitochondrial Cytochrome b and Cytochrome c Oxidase II Genes with Decreased Mitochondrial DNA Copy Numbers in the APP/PS1 Transgenic Mouse Model of Alzheimer's Disease.

Neurochem Res 2021 Mar 12;46(3):564-572. Epub 2021 Feb 12.

Department of Neurology Medicine, The Second Hospital, Cheeloo College of Medicine, Shandong University, 247th of Beiyuan Rd., Jinan, Shandong, China.

Alzheimer's disease (AD) is the most common cause of dementia. Increasing evidence shows that mitochondrial DNA (mtDNA) methylation plays an essential role in many diseases related to mitochondrial dysfunction. Since mitochondrial impairment is a key feature of AD, mtDNA methylation may also contribute to AD, but few studies have addressed this issue. Methylation changes of the mitochondrial cytochrome b (CYTB) and cytochrome c oxidase II (COX II) genes in AD have not been reported. We analyzed mtDNA methylation changes of the CYTB and COX II genes in an APP/PS1 transgenic mouse model of AD using pyrosequencing. We examined mtDNA copy numbers and the levels of expression by quantitative real-time PCR. Average methylation levels of different CpG sites were ≤ 4.0%. Methylated mtDNA accounted for only a small part of the total mtDNA. We also observed hypermethylation of mitochondrial CYTB and COX II genes with decreased mtDNA copy numbers and expression in the hippocampi of APP/PS1 transgenic mice. mtDNA methylation may play an important role in AD pathology, which may open a new window for AD therapy.
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http://dx.doi.org/10.1007/s11064-020-03192-yDOI Listing
March 2021

MiR-452-5p mediates the proliferation, migration and invasion of hepatocellular carcinoma cells via targeting COLEC10.

Per Med 2021 Mar 10;18(2):97-106. Epub 2021 Feb 10.

Department of Hepatobiliary Surgery, Tangshan Gongren Hospital, Tangshan 063000, PR China.

This study explored the potential function of miR-452-5p in hepatocellular carcinoma (HCC) and clarified the mechanism underlying HCC progression. Real-time quantitative PCR was used to detect miR-452-5p and COLEC10 mRNA expression in HCC, western blot was performed to test COLEC10 protein expression. The regulatory mechanism of miR-452-5p/COLEC10 in HCC cells was explored using CCK-8, wound healing assay, Transwell and dual-luciferase reporter assay. MiR-452-5p was greatly upregulated in HCC cells, and it served as an oncogene playing an active role in HCC cell proliferation, migration and invasion. COLEC10 was identified as the target of miR-452-5p in HCC attenuating the promoting effect of miR-452-5p on HCC cells upon overexpression. MiR-452-5p can promote the progression of HCC via targeting COLEC10.
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http://dx.doi.org/10.2217/pme-2020-0027DOI Listing
March 2021

Pharmacokinetic Comparability of Risankizumab Formulations in Prefilled Syringe and Auto-injector for Subcutaneous Injection.

Clin Ther 2021 Mar 3;43(3):629-636. Epub 2021 Feb 3.

Department of Clinical Pharmacology and Pharmacometrics, AbbVie Inc, North Chicago, IL, USA. Electronic address:

Purpose: Risankizumab is a humanized immunoglobulin G1 monoclonal antibody that inhibits the p19 subunit of interleukin 23 from interacting with its receptor for the treatment of moderate to severe plaque psoriasis. The aim of this Phase I biopharmaceutics bridging study was to evaluate the pharmacokinetic comparability, immunogenicity, and tolerability of the risankizumab 90 mg/mL prefilled syringe (PFS) and the risankizumab 150 mg/mL PFS and auto-injector (AI) in healthy subjects.

Methods: Healthy subjects received one 150-mg dose of risankizumab in 1 of 3 ways (226 subjects randomized 3:3:1 to 3 treatment arms): 150 mg/mL by PFS × 1 SC injection, 90 mg/mL by PFS × 2 SC injections, or 150 mg/mL by AI × 1 SC injection, and were followed up for 140 days after dosing for the collection of pharmacokinetic, immunogenicity, and tolerability data.

Findings: Risankizumab concentration-time profiles overlapped with comparable pharmacokinetic parameters across all treatment arms, indicating similar pharmacokinetic characteristics. The CIs with both formulations and forms of administration were within the bioequivalence range of 0.80-1.25 across all measures of exposure. The prevalence of treatment-emergent anti-drug antibodies and the percentages of subjects who reported at least 1 treatment-emergent adverse event were comparable across all treatment arms.

Implications: Bioequivalence was established between risankizumab 150 mg/mL PFS and 90 mg/mL PFS, and between 150 mg/mL PFS and AI, along with comparable immunogenicity profiles across all 3 treatment arms. Risankizumab 150 mg SC delivered by PFS or AI was well tolerated, with comparable safety profiles across all treatment arms, and no new safety risks were identified.
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http://dx.doi.org/10.1016/j.clinthera.2021.01.009DOI Listing
March 2021

Sequential use of EGFR-tyrosine kinase inhibitors based upon EGFR mutation evolution achieves long-term control in a non-small cell lung cancer patient: a case report.

Ann Palliat Med 2021 Feb 2. Epub 2021 Feb 2.

Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Tyrosine kinase inhibitor (TKI) has greatly improved the survival of non-small cell lung cancer (NSCLC) with epidermal growth factor receptor (EGFR)-TKI sensitive mutations. However, TKI resistance constantly occur, although multiple lines of different generations of TKIs are adopted during the progression. For example, in the case when T790M, which is the most common resistance mechanism of first generation TKIs, occurs, alteration to osimertinib (the third generation TKI) could always be effective. Unfortunately, some cases gradually become resistant even to osimertinib leaving limited therapy choice for clinical practitioners. Few cases have been reported in the situation after EGFR tertiary mutations occurred, such as C797S, G724S, etc. Herein, we report the first clinical evidence that sequential therapy with erlotinib, osimertinib, afatinib plus endostar, brigatinib plus cetuximab, almonertinib, almonertinib plus afatinib achieved long-term control in a NSCLC patient demonstrating EGFR 19Del/T790M/G724S/cis-C797S evolution in response to TKI treatment. EGFR targeted therapy introduced successful management for more than 36 months until now. ctDNA NGS was performed at the time of important clinical event. The EGFR 19Del was discovered in October 2017, and erlotinib was administered for 10 months with PR in the beginning. Then T790M was detected and osimertinib was used for 9 months with SD condition. Subsequently, EGFR G724S was identified in ctDNA with the remaining 19Del and loss of T790M. Afatinib plus endostar was administered and PR was achieved after 1.5 months. PD occurred 6 months later with the emergence of EGFR cis-C797S. Then brigatinib plus cetuximab was chosen and lasted for 4 months with the best response of SD. And then EGFR 19Del and T790M were still detected with loss of G724S and C797S. Almonertinib, another third-generation TKI, was administered for 3 months with SD condition. Finally, 19Del/T790M/G724S/cis-C797S recurred, and whole dose of almonertinib plus afatinib was prescribed until now with a PR at 2 months until now. The side effect was acceptable during the whole period of therapies. Plasma ctDNA NGS provided information of EGFR mutation evolution and inform appropriate therapy regimen during the progression.
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http://dx.doi.org/10.21037/apm-20-1477DOI Listing
February 2021

Association study of hypertension susceptibility genes , and with preeclampsia in Chinese Han population.

J Matern Fetal Neonatal Med 2021 Jan 24:1-9. Epub 2021 Jan 24.

Department of Obstetrics, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, P.R. China.

Objective: Preeclampsia (PE) is a disorder that occurs during the pregnancy and could affect the maternal and perinatal mortality as well as morbidity. The aim of our study is to investigate the associations between the hypertension susceptibility genes , and with PE in Chinese Han population.

Methods: A case-control study including 178 PE patients and 202 healthy controls was conducted to assess the associations between three loci ( rs155524, rs2932538 and rs4373814) and PE. The TaqMan probe assay was applied for genotyping in our study. Quantitative real-time PCR was performed to detect the mRNA expression levels of ITGA9, MOV10 and CACNB2. ELISA was carried out to detect the concentration of serum sFlt-1 or PLGF.

Results: Our study detected no significant differences in allelic frequencies of three SNPs between PE patients and healthy controls. In the genetic model, the results showed that the patients with rs155524 GA or AA genotypes had a higher risk of PE development compared to those with GG genotype in codominant model. And PE patients had a higher frequency of GA + AA genotypes based on the dominant model. Subgroup analysis showed rs155524 was associated with early-onset PE but not with late-onset PE. No association was observed between and with PE in any genetic model and subgroup analysis. Quantitative real-time PCR results showed that ITGA9 mRNA expression level was apparently increased in the placental tissues of PE patients. In addition, ITGA9 expression levels of GA + AA subjects were apparently higher than that in the genotype GG of placental tissues. sFlt-1/PLGF ratio was higher in GA + AA subjects than that in GG subjects. Regression analysis revealed that ratio of sFlt-1/PLGF was positively correlated with ITGA9 mRNA expression level.

Conclusion: This study has identified is a promising candidate susceptibility gene for early-onset PE. Our findings demonstrated that the high expression of ITGA9 might be associated with an increased risk of PE.
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http://dx.doi.org/10.1080/14767058.2021.1876022DOI Listing
January 2021

Quantitative Proteomics Reveals that GmENO2 Proteins Are Involved in Response to Phosphate Starvation in the Leaves of L.

Int J Mol Sci 2021 Jan 18;22(2). Epub 2021 Jan 18.

College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Soybean ( L.) is a major crop providing important source for protein and oil for human life. Low phosphate (LP) availability is a critical limiting factor affecting soybean production. Soybean plants develop a series of strategies to adapt to phosphate (Pi) limitation condition. However, the underlying molecular mechanisms responsible for LP stress response remain largely unknown. Here, we performed a label-free quantification (LFQ) analysis of soybean leaves grown under low and high phosphate conditions. We identified 267 induced and 440 reduced differential proteins from phosphate-starved leaves. Almost a quarter of the LP decreased proteins are involved in translation processes, while the LP increased proteins are accumulated in chlorophyll biosynthetic and carbon metabolic processes. Among these induced proteins, an enolase protein, GmENO2a was found to be mostly induced protein. On the transcriptional level, and but not or were dramatically induced by phosphate starvation. Among 14 enolase genes, only and genes contain the P1BS motif in their promoter regions. Furthermore, was specifically induced in the overexpressing soybean plants. Our findings provide molecular insights into how soybean plants tune basic carbon metabolic pathway to adapt to Pi deprivation through the ENO2 enzymes.
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http://dx.doi.org/10.3390/ijms22020920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831476PMC
January 2021

BiPO -Derived 2D Nanosheets for Efficient Electrocatalytic Reduction of CO to Liquid Fuel.

Angew Chem Int Ed Engl 2021 Mar 3;60(14):7681-7685. Epub 2021 Mar 3.

Key Laboratory for Ultrafine Materials of Ministry of Education, Shanghai Engineering Research Center of Hierarchical Nanomaterials, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Materials Science and Engineering, East China University of Science & Technology, Shanghai, 200237, China.

The electrochemical reduction of carbon dioxide (CO ) to high-value liquid fuel with high selectivity is appealing for energy conversion and storage. Here we report a bismuth phosphate (BiPO ) derived 2D nanosheet-like electrocatalyst that efficiently converts CO into liquid-phase formate. The catalyst presents a formate Faradaic efficiency of over 90 % and a cathodic energy efficiency of 73 % at an industrially relevant current density of 200 mA cm in the flow cell. The in situ generation of the Bi-O active species on the catalyst surface was determined via operando Raman measurement. Morphological and X-ray photoelectron spectroscopy analyses reveal the origin of the high activity for the electrosynthesis of formate from CO and water: the 2D structure together with the abundant insertion of oxygen atoms in the surface of the BiPO -derived nanosheets.
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http://dx.doi.org/10.1002/anie.202014341DOI Listing
March 2021

Effect of electroacupuncture on the kisspeptin system in a pubertal rat model of polycystic ovary syndrome.

Acupunct Med 2021 Jan 7:964528420971299. Epub 2021 Jan 7.

Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China.

Objective: To explore the effects and mechanism of action of electroacupuncture (EA) in a rat model of pubertal polycystic ovary syndrome (PCOS).

Methods: Female offspring of Sprague-Dawley rats receiving dihydrotestosterone (DHT) during pregnancy (days 16-19), as a model of prenatal androgenization, were divided randomly into three groups: model group (M), EA group, and sham acupuncture (SA) group (n = 8 each). A normal (N) group comprising female offspring of healthy pregnant rats not receiving DHT (n = 8) was added. EA was administered at CV6 and bilateral SP6/ST36 with 2 Hz frequency and 2 mA intensity. SA consisted of superficial needling at different locations without electrical stimulation.

Results: EA improved the disturbed estrous cycles, while it could not be concluded that SA was effective in this respect. EA improved ovarian morphology including the number of corpora lutea and area of the ovary, whereas SA did not. However, both EA and SA attenuated the increased luteinizing hormone and decreased estradiol and gonadotropin-releasing hormone levels in the serum of PCOS model rats. Levels of testosterone, follicle-stimulating hormone, and progesterone did not significantly differ between groups. EA and SA alleviated the upregulation of kisspeptin protein and mRNA levels in the hypothalamus and kisspeptin protein level in the arcuate nucleus (ARC). No differences were found between groups in protein or mRNA expression of dynorphin (DYN) or neurokinin B (NKB) in the hypothalamus. Co-expression of kisspeptin, NKB, and DYN were observed in ARC. The GnRH level in the median eminence decreased and could be rescued by EA and SA. Intriguingly, kisspeptin levels in the granulosa cells of the ovary decreased in the model group and could be rescued by EA but not SA. Levels of kisspeptin, NKB, and DYN protein and mRNA in the ovary did not differ between any groups.

Conclusion: Both EA and SA appeared to improve symptoms of PCOS at puberty by modulating the kisspeptin system in the hypothalamus. EA also had an effect on ovarian kisspeptin expression and a more comprehensive effect with respect to improving PCOS at puberty than SA.
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http://dx.doi.org/10.1177/0964528420971299DOI Listing
January 2021

Loss of MBD2 affects early T cell development by inhibiting the WNT signaling pathway.

Exp Cell Res 2021 01 30;398(1):112400. Epub 2020 Nov 30.

Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. Electronic address:

DNA methylation alters the expression of certain genes without any alteration to the DNA sequence and is a dynamic process during normal hematopoietic differentiation. As an epigenetic regulator, methyl-CpG-binding domain protein 2 (MBD2) is an important member of the MBD protein family and is acknowledged as a "reader" of DNA methylation. We used a mouse model to study the effects of MBD2 on the early development of T cells. Here, we found that MBD2 deficiency led to retardation of T cell differentiation at the DN3 stage. Meanwhile, decreased proliferative capacity and increased apoptosis were detected in Mbd2 DN thymocytes. Furthermore, we found the WNT pathway was significantly down-regulated in Mbd2 DN thymocytes: DKK1 (Dickkopf-1) expression was significantly increased, while TCF7 (transcription factor 7) and c-MYC were down-regulated. Thus, these findings established that MBD2 acted as a dominant regulator to imprint DN T cell development via the WNT pathway.
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http://dx.doi.org/10.1016/j.yexcr.2020.112400DOI Listing
January 2021

Promotes Colon Cancer Cell Proliferation by Targeted Expression Inhibitionn.

Cancer Manag Res 2020 6;12:11341-11350. Epub 2020 Nov 6.

Department of Hepatobiliary, Pancreatic and Gastric Surgery, Jinhua Guangfu Hospital, Jinhua 321000, People's Republic of China.

Objective: Colon cancer (CC) is the third most common cancer with a high rate of incidence and mortality. Therefore, it is highly necessary to explore novel targets of CC.

Methods: The miRNA-seq and RNA-seq data of CC were accessed from the TCGA database. Differential analysis was performed using the "edgeR" package to identify differentially expressed miRNAs (DE_miRNAs). The downstream target genes of the target miRNA were then predicted by miRNA target prediction databases to identify the target mRNA. Normal colon cell line CCD-18Co and CC cell lines HCT-116, HT-29, SW620 and SW480 were chosen, and qRT-PCR was conducted to detect expression in these cell lines. qRT-PCR and Western blot were carried out to determine mRNA and protein expression, respectively. Dual-luciferase reporter gene assay was performed to verify the targeting relationship between and 3'UTR. CCK-8 assay and colony formation assay were carried out to detect cell proliferation. Meanwhile, tumor xenograft model in nude mice was constructed to assess CC cell tumorigenic ability in vivo.

Results: was markedly up-regulated in CC tissue. CC cell proliferation and in vivo tumorigenic ability were suppressed by silencing but promoted by over-expression. was significantly down-regulated in cancer tissue. Dual-luciferase reporter gene assay indicated that could bind to 3'UTR. expression was noticeably elevated upon deficiency but significantly inhibited upon over-expression. CCK-8 and colony formation assay suggested that facilitated CC cell proliferation by silencing .

Conclusion: promotes CC cell proliferation by targeted silencing .
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http://dx.doi.org/10.2147/CMAR.S256670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7661787PMC
November 2020

miR-9-5p facilitates hepatocellular carcinoma cell proliferation, migration and invasion by targeting ESR1.

Mol Cell Biochem 2021 Feb 27;476(2):575-583. Epub 2020 Oct 27.

Department of Hepatobiliary Surgery, Tangshan Gongren Hospital, No. 27 Cultural Road, Lubei District, Tangshan, 063000, Hebei, China.

The study aimed to explore the relationship between miR-9-5p and ESR1, and clarify the underlying functional mechanism in the occurrence and development of hepatocellular carcinoma (HCC). Expression data including miRNAs and mRNAs of HCC downloaded from TCGA database were processed for differential analysis, and corresponding clinical data were collected for survival analysis to identify the target miRNA miR-9-5p. Bioinformatics databases were applied for predicting downstream target mRNAs of miR-9-5p. qRT-PCR was used to evaluate expression of miR-9-5p. Western blot was used to detect protein expression of ESR1. MTT, wound healing assay and Transwell assay were used to detect HCC cell proliferation, migration and invasion, respectively. Dual-luciferase reporter gene assay was used to identify the targeting relationship between miR-9-5p and ESR1. Research suggested that miR-9-5p was highly expressed in HCC cells but ESR1 was poorly expressed. Overexpression of miR-9-5p could improve the proliferation, invasion and migration of cells. Dual-luciferase reporter assay showed that ESR1 was the downstream target of miR-9-5p in HCC. Overexpression of miR-9-5p markedly reduced ESR1 mRNA and protein levels in HCC cells, whereas inhibition of miR-9-5p expression produced the contrary results. Silencing ESR1 could noticeably reverse the effect of miR-9-5p knockdown on the proliferation, migration and invasion of HCC cells. As an oncogene, miR-9-5p fostered the proliferation, migration and invasion of HCC cells by targeting and inhibiting ESR1 expression.
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http://dx.doi.org/10.1007/s11010-020-03927-zDOI Listing
February 2021

Efficacy of low-level light therapy for improving healing of diabetic foot ulcers: A systematic review and meta-analysis of randomized controlled trials.

Wound Repair Regen 2021 01 30;29(1):34-44. Epub 2020 Oct 30.

Alice Lee Centre for Nursing Studies, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.

Diabetic foot ulcers are prevalent among patients with diabetes and negatively affect mortality and life expectancy. This study aimed to synthesize and systematically review the best evidence to assess the efficacy of low-level light therapy in improving healing of diabetic foot ulcers. We search CINAHL, Cochrane Library, EMBASE, ProQuest, PubMed, Scopus, and Web of Science from inception until September 30, 2019. Meta-analysis was performed using the Comprehensive Meta-analysis 3.0 software. Overall effect was measured using Hedges' g and determined using the Z-statistic at a significance level of P < .05. Heterogeneity was assessed using χ and I statistics. Twelve randomized controlled trials were included. Meta-analysis revealed that 30.90% of the ulcer area was significantly reduced in the therapy group compared with the control group (Z = 3.95, P < .001) with a very large effect (g = 2.81). A 4.2 cm reduction of the ulcer area was observed in the therapy group compared with the control group (Z = 2.17, P = .03) with a very large effect (g = 1.37). In addition, diabetic foot ulcers in the therapy group was 4.65 times more likely to heal completely than those in the control group (Z = 3.02, P = .003). Low-level light therapy accelerates wound healing and reduces the size of diabetic foot ulcers. However, our review does not allow any recommendation for the best treatment parameters required to achieve improved healing. Future trials need to include a good design and large sample size in defining the optimal treatment parameters for ulcers of different sizes.
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http://dx.doi.org/10.1111/wrr.12871DOI Listing
January 2021

HAS2-AS1 Acts as a Molecular Sponge for miR-137 and Promotes the Invasion and Migration of Glioma Cells by Targeting EZH2.

Cell Cycle 2020 Nov 16;19(21):2826-2835. Epub 2020 Oct 16.

The Fourth Department of Neurosurgery, Tangshan Gongren Hospital , Tangshan, China.

This study aims to explore the molecular mechanism by which HAS2-AS1 acts as a ceRNA to promote the invasion and migration of glioma cells, which will provide a novel potential target for the targeted therapy of glioma. Gene expression profiles and corresponding clinical data were accessed from the TCGA_LGG and TCGA_GBM databases and then differential analysis was conducted using the "edgeR" package. miRDB, miRTarBase and TargetScan databases were employed to predict target genes and sequentially a ceRNA network was constructed. Quantitative real-time PCR was performed to detect gene expression in glioma cells. Transwell assay was operated to assess cell migratory and invasive abilities. Western blot was conducted to evaluate the protein expression. Dual-luciferase reporter assay and RNA immunoprecipitation experiment were performed to validate the targeting relationship between genes. HAS2-AS1 was markedly upregulated in glioma, and the overall survival time of patients with high HAS2-AS1 expression was significantly shorter than that of patients with low one. Silencing HAS2-AS1 inhibited the migration and invasion of glioma cells, while overexpressing HAS2-AS1 produced opposite results. miR-137 was validated as a direct target of and negatively regulated by HAS2-AS1. Further exploration of the downstream target gene indicated that EZH2 competed with HAS2-AS1 to interact with miR-137. Suppressing miR-137 or up-regulating EZH2 reversed the impact of HAS2-AS1 knockdown on glioma cell invasion and migration. HAS2-AS1 regulates EZH2 by sponging miR-137 for the migratory and invasive abilities of glioma cells, which provides a new idea for exploring metastasis mechanism of glioma.
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http://dx.doi.org/10.1080/15384101.2020.1826237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714441PMC
November 2020

Game-based brain training for improving cognitive function in community-dwelling older adults: A systematic review and meta-regression.

Arch Gerontol Geriatr 2021 Jan - Feb;92:104260. Epub 2020 Sep 19.

Alice Lee Centre for Nursing Studies, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. Electronic address:

Background: Given that increasing aging is associated with a natural decline in cognitive function, identifying effective interventions that can help to prevent cognitive decline in older adults is a research priority.

Objective: To synthesize the best evidence to assess the effectiveness of game-based brain training in improving cognitive function and to evaluate the preferred design features of the intervention.

Methods: Twelve databases, trial registries, and gray literature resources were systematically searched for in randomized controlled trials. Meta-analysis and random-effects meta-regression were conducted using Comprehensive Meta-analysis Software 3.0. Overall effect was measured using Hedges's g and determined using Z-statistics. Cochran's Q test and I were used to investigate heterogeneity. The Grading of Recommendation, Assessment, Development, and Evaluation system was used to assess overall quality of evidence.

Results: Fifteen trials among 759 older adults were conducted. Meta-analysis revealed that game-based brain training significantly improved processing speed (g = 0.23), selective attention (g = 0.40), and short-term memory (g = 0.35) versus a control group. Our subgroup analyses emphasized that non-time pressure games, multiplayer, computer platform, provider support, sessions ≤ 3 times per week for ≤ 60 min. each comprised a preferable design. Meta-regression identified game design (β = 0.211, p = 0.008) that had statistically significant effects on processing speed. Egger's regression asymmetry test (p = 0.293) suggested no publication bias.

Conclusions: Game-based brain training can be considered a supplementary intervention for improving cognitive functions in community-dwelling older adults. Future trials should use well-designed trials with large sample sizes.
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http://dx.doi.org/10.1016/j.archger.2020.104260DOI Listing
February 2021

Humanoid robot-assisted interventions among children with diabetes: A systematic scoping review.

Int J Nurs Stud 2020 Nov 18;111:103749. Epub 2020 Aug 18.

Associate Professor, Alice Lee Centre for Nursing Studies, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. Electronic address:

Background: Although the humanoid robot is highly engaging for children, whether humanoid robot-assisted interventions could help in diabetes management is still unclear.

Objectives: This review aimed to elucidate the roles of humanoid robot in managing diabetes and observe the effects of humanoid robot-assisted interventions among children with type I diabetes mellitus.

Methods: Arksey and O'Malley's methodological framework was used to conduct this scoping review.

Data Resource: We conducted a comprehensive search in 10 databases, including IEEE Xplore Digital Library, CINAHL, Cochrane Library, EMBASE, PubMed, Scopus, Web of Science, ProQuest, WanFang, and CNKI, from May 1, 1989 to February 1, 2020. The reference lists of key texts were examined and citation chaining was conducted. In addition, our team searched gray literature and relevant journals to maximise the searching.

Review Methods: Two independent reviewers examined all full articles for fitness with the eligibility criteria. Items of data extraction included the characteristics of articles, description of intervention, and mechanism of humanoid robot in improving the management of children with diabetes.

Results: A total of 2,755 articles were retrieved, 22 of which were included from five countries across different ethnicities. Five roles of humanoid robots in managing diabetes among children with type I diabetes mellitus were identified: disease manager, educator, advocator, emotional supporter, and communicator. To some extent, effects seem to be related to the positive improvement of affective, experiential, relational, cognitive, and behavioral perspectives from humanoid robot-assisted intervention.

Conclusions: The roles of humanoids have been designed to perform their multidomain functionality. Although many positive effects were observed, the majority of selected articles have methodological issues. Future studies need an improved methodology for large-scale controlled trials.
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http://dx.doi.org/10.1016/j.ijnurstu.2020.103749DOI Listing
November 2020

Decreased prealbumin level is associated with increased risk for mortality in elderly hospitalized patients with COVID-19.

Nutrition 2020 10 3;78:110930. Epub 2020 Jul 3.

Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Objectives: High-risk patients ≥65 y of age with coronavirus disease 2019 (COVID-19) tended to have lower serum prealbumin concentrations. The aim of this study was to investigate the association of prealbumin at baseline on COVID-19-related mortality in elderly patients (≥65 y of age).

Methods: We non-selectively and consecutively collected participants from Tongji Hospital in Wuhan from January 17 to February 17, 2020. Univariate and multivariate logistic regression models were employed to evaluate the correlation between prealbumin and in-hospital outcomes (in-hospital mortality, admission to the intensive care unit [ICU], and mechanical ventilation) in elderly patients with COVID-19. Linear trend was performed by entering the median value of each category of prealbumin tertile as a continuous variable and was visually confirmed by using generalized additive models. Interaction and stratified analyses were conducted as well.

Results: We included 446 elderly patients with COVID-19 in the final analyses. In-hospital mortality was 14.79%. Of the 446 patients, 15.47% were admitted to the ICU and 21.3% required mechanical ventilation. Compared with patients in the highest tertile, the prealbumin of patients in the lowest tertile had a 19.09-fold higher risk for death [odds ratio (OR), 20.09; 95% confidence interval (CI), 3.62-111.64; P = 0.0006], 25.39-fold higher risk for ICU admission (OR, 26.39; 95% CI, 4.04-172.39; P = 0.0006), and 1.8-fold higher risk for mechanical ventilation (OR, 2.8; 95% CI, 1.15-6.78; P = 0.0227) after adjustment for potential confounders. There was a linear trend correlation between serum prealbumin concentration and risk for in-hospital mortality, ICU admission, and mechanical ventilation in elderly patients with COVID-19 infection.

Conclusion: Prealbumin is an independent risk factor of in-hospital mortality for elderly patients with COVID-19. Assessment of prealbumin may help identify high-risk individuals ≥65 y of age with COVID-19.
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http://dx.doi.org/10.1016/j.nut.2020.110930DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333599PMC
October 2020

Short-sighted deep learning.

Phys Rev E 2020 Jul;102(1-1):013307

School of Electrical and Information Engineering, University of the Witwatersrand, Wits 2050, South Africa.

A theory explaining how deep learning works is yet to be developed. Previous work suggests that deep learning performs a coarse graining, similar in spirit to the renormalization group (RG). This idea has been explored in the setting of a local (nearest-neighbor interactions) Ising spin lattice. We extend the discussion to the setting of a long-range spin lattice. Markov-chain Monte Carlo (MCMC) simulations determine both the critical temperature and scaling dimensions of the system. The model is used to train both a single restricted Boltzmann machine (RBM) network, as well as a stacked RBM network. Following earlier Ising model studies, the trained weights of a single-layer RBM network define a flow of lattice models. In contrast to results for nearest-neighbor Ising, the RBM flow for the long-ranged model does not converge to the correct values for the spin and energy scaling dimension. Further, correlation functions between visible and hidden nodes exhibit key differences between the stacked RBM and RG flows. The stacked RBM flow appears to move toward low temperatures, whereas the RG flow moves toward high temperature. This again differs from results obtained for nearest-neighbor Ising.
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http://dx.doi.org/10.1103/PhysRevE.102.013307DOI Listing
July 2020

The transcription factor USF1 promotes glioma cell invasion and migration by activating lncRNA HAS2-AS1.

Biosci Rep 2020 08;40(8)

The Fourth Department of Neurosurgery, Tangshan Gongren Hospital, Tangshan, Hebei, China.

Objective: The role of lncRNAs in tumor has been widely concerned. The present study took HAS2-AS1 (the antisense RNA 1 of HAS2) as a starting point to explore its expression in glioma and its role in the process of migration and invasion, providing a strong theoretical basis for mining potential therapeutic targets of glioma.

Methods: Clinical data of glioma were obtained from The Cancer Genome Atlas (TCGA) database and differentially expressed lncRNAs were analyzed by edgeR. The hTFtarget database was used to predict the upstream transcription factors of HAS2-AS1 and the JASPAR website was used to predict the binding sites of human upstream transcription factor 1 (USF1) and HAS2-AS1. qRT-PCR was used to detect the expressions of HAS2-AS1 and USF1 in glioma tissues and cell lines. The effects of silencing HAS2-AS1 on the migration and invasion of cancer cells were verified by wound healing and Transwell invasion assays. The chromatin immunoprecipitation (ChIP) and dual luciferase reporter assays were applied to demonstrate the binding of USF1 and HAS2-AS1 promoter region. Western blot was used to detect the expressions of epithelial-mesenchymal transition (EMT)-related proteins.

Results: HAS2-AS1 was highly expressed in glioma tissues and cells, and was significantly associated with poor prognosis. Silencing HAS2-AS1 expression inhibited glioma cell migration, invasion and EMT. USF1 was highly expressed in glioma and positively correlated with HAS2-AS1. The transcription of HAS2-AS1 was activated by USF1 via binding to HAS2-AS1 promoter region, consequently potentiating the invasion and migration abilities of glioma cells.

Conclusion: These results suggested that the transcription factor USF1 induced up-regulation of lncRNA HAS2-AS1 and promoted glioma cell invasion and migration.
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http://dx.doi.org/10.1042/BSR20200487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442972PMC
August 2020

Ectopic thyroid as multiple nodules in bilateral lung lobes: a case report.

Gland Surg 2020 Jun;9(3):806-811

Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Ectopic thyroid rarely occurs in the thoracic or abdominal cavity, especially in the lung. Here, we describe an intrapulmonary thyroid tissue case in bilateral lung lobes without thyroid malignant history, which was found during a routine physical examination. A 37-year-old Chinese female showed multiple pulmonary nodules appearing in bilateral lung lobes on a computed tomography (CT) scan during a routine medical examination. To rule out the possibility of lung metastasis from occult cancer, a percutaneous lung biopsy was performed on a larger nodule. Histopathological examination confirmed that the nodule was completely consistent with normal thyroid tissue, which prevented the patient from requiring any unnecessary treatment. During the patient's follow-up, there was no abnormal thyroid function, and there was no change in the size of the nodule in the lung during serial CT scan. Currently, there is no guidance or consensus on the diagnosis of ectopic thyroid with bilateral multiple pulmonary nodules due to its rarity in clinical practice. When such pulmonary nodules are encountered, very careful diagnosis and follow-up should be performed.
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http://dx.doi.org/10.21037/gs-19-499DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347806PMC
June 2020

Acute Kidney Injury Is Associated With In-hospital Mortality in Older Patients With COVID-19.

J Gerontol A Biol Sci Med Sci 2021 02;76(3):456-462

Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: The epidemic of COVID-19 presents a special threat to older adults. However, information on kidney damage in older patients with COVID-19 is limited. Acute kidney injury (AKI) is common in hospitalized adults and associated with poor prognosis. We sought to explore the association between AKI and mortality in older patients with COVID-19.

Methods: We conducted a retrospective, observational cohort study in a large tertiary care university hospital in Wuhan, China. All consecutive inpatients older than 65 years with COVID-19 were enrolled in this cohort. Demographic data, laboratory values, comorbidities, treatments, and clinical outcomes were all collected. Data were compared between patients with AKI and without AKI. The association between AKI and mortality was analyzed.

Results: Of 1764 in-hospital patients, 882 older adult cases were included in this cohort. The median age was 71 years (interquartile range: 68-77), 440 (49.9%) were men. The most presented comorbidity was cardiovascular diseases (58.2%), followed by diabetes (31.4%). Of 882 older patients, 115 (13%) developed AKI and 128 (14.5%) died. Patients with AKI had higher mortality than those without AKI (68 [59.1%] vs 60 [7.8%]; p < .001). Multivariable Cox regression analysis showed that increasing odds of in-hospital mortality are associated with higher interleukin-6 on admission, myocardial injury, and AKI.

Conclusions: Acute kidney injury is not an uncommon complication in older patients with COVID-19 but is associated with a high risk of death. Physicians should be aware of the risk of AKI in older patients with COVID-19.
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http://dx.doi.org/10.1093/gerona/glaa181DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7454401PMC
February 2021

Efficacy of five-element music interventions in perinatal mental health and labor pain: A meta-analysis.

Complement Ther Clin Pract 2020 Aug 2;40:101217. Epub 2020 Jul 2.

Graduate College, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, PR China.

Objective: To systematically review the efficacy of five-element music interventions in perinatal mental health and labor pain. To provide evidence for future research on Chinese Traditional Music Interventions.

Methods: An electronically search was conducted in Web of Science, PubMed, EMbase, The Cochrane Library, CNKI databases, WanFang Data and VIP Database for Chinese Technical Periodicals from inception to January 07, 2020 to collect randomized controlled trials (RCTs) on five-element music interventions for prenatal, perinatal and postpartum women. Literature screening, data extraction, and the risk of bias assessment of all eligible studies were conducted by two reviewers independently. Then, meta-analysis was performed by RevMan 5.3 software.

Results: A total of 13 RCTs involving 2387 patients were included. The results of meta-analysis showed that five-element music could significantly improve the depression levels (SMD = -2.03, 95%CI: -2.88 to -1.19, P < 0.00001), anxiety levels (SMD = -3.49, 95%CI: -4.47 to -2.24, P < 0.00001) and severe anxiety rate (OR = 0.42, 95%CI: 0.19 to 0.94, P = 0.04) of perinatal women. Additionally, five-element music produced significant effects on labor pain (MD = -0.67, 95%CI: -0.82 to -0.52, P < 0.00001), labor duration (MD = -0.85, 95%CI: -1.07 to -0.63, P < 0.00001), the hemorrhage 2 h after parturition (MD = -19.55, 95%CI: -35.56 to -3.54, P = 0.02) and the Serum DYN expression level (MD = 1.32, 95%CI: 0.23 to 2.40, P = 0.02).

Conclusions: Five-element music may be efficacious in improving perinatal women' depression, anxiety, labor pain, labor duration, the hemorrhage 2 h after parturition and the Serum DYN expression level. Because of the limitation of quantity and quality of included studies, more high-quality studies were needed to confirm the above conclusion.
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http://dx.doi.org/10.1016/j.ctcp.2020.101217DOI Listing
August 2020

Physiological and transcriptomic analyses of brassinosteroid function in moso bamboo (Phyllostachys edulis) seedlings.

Planta 2020 Jul 25;252(2):27. Epub 2020 Jul 25.

Basic Forestry and Proteomics Research Center, Forestry College, Fujian Agriculture and Forestry University, Fuzhou, China.

Main Conclusion: This study demonstrates that brassinosteroid is essential for seedling and shoot growth in moso bamboo. The shoot of moso bamboo is known to grow extremely fast. The roles of phytohormones in such fast growth of bamboo shoot remain unclear. Here we reported that endogenous brassinosteroid (BR) is a major factor promoting bamboo shoot internode elongation. Reducing endogenous brassinosteroid level by its biosynthesis inhibitor propiconazole stunted shoot growth in seedling stage, whereas exogenous BR application promoted scale leaf elongation and the inclination of lamina joint of leaves and scale leaves. Genome-wide transcriptome analysis identified hundreds of genes whose expression levels are altered by BR and propiconazole in shoots and roots of bamboo seedling. The data show that BR regulates cell wall-related genes, hydrogen peroxide catabolic genes, and auxin-related genes. Our study demonstrates an essential role of BR in fast growth bamboo shoots and identifies a large number of BR-responsive genes in bamboo seedlings.
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http://dx.doi.org/10.1007/s00425-020-03432-zDOI Listing
July 2020

MiR-451a ameliorates alcoholic hepatitis via repressing HDAC8-mediated proinflammatory response.

Kaohsiung J Med Sci 2020 Nov 9;36(11):904-910. Epub 2020 Jul 9.

Department of Physiology, Inner Mongolia Medical University, Hohhot, Inner Mongolia Autonomous Region, China.

Alcoholic hepatitis (AH) is identified as an inflammatory syndrome with high morbidity and mortality as a result of severe hepatocellular dysfunction and liver injury. Accumulated studies indicated that miRNAs are involved in AH. The potential effect of miR-451a in AH mice was examined in the current study. A mice AH model was established and the miR-451a expression in AH mice compared with the sham group was tested by real-time polymerase chain reaction (qRT-PCR). AH mice were injected with pre-miR-451a lentivirus for miR-451a overexpression and histone deacetylase (HDAC8) lentivirus for HDAC8 overexpression in AH mice. The underlying mechanisms were explored by searching the potential target genes of miR-451a in miRanda database and then we confirmed this. We found that miR-451a expression was significantly decreased in AH mice compared with the sham group. Moreover, miR-451a overexpression alleviated alcohol-induced liver inflammation and injuries of AH mice. Additionally, further mechanism exploration disclosed that HDAC8 was a target of miR-451a. The protective effect of miR-451a on AH in AH mice was abolished by HDAC8 overexpression. In summary, miR-451a ameliorates AH via repressing HDAC8-mediated proinflammatory response.
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http://dx.doi.org/10.1002/kjm2.12272DOI Listing
November 2020