Publications by authors named "Lina Zhang"

957 Publications

Biocompatible, antibacterial and anti-inflammatory zinc ion cross-linked quaternized cellulose‑sodium alginate composite sponges for accelerated wound healing.

Int J Biol Macromol 2021 Sep 14. Epub 2021 Sep 14.

College of Chemistry and Molecular Sciences, Hubei Engineering Center of Natural Polymer-based Medical Materials, Wuhan University, Wuhan 430072, China. Electronic address:

Bacterial infection has become one of the most challenges for wound healing, which causes serious inflammatory response and delays the healing process. Herein, a novel sponge with excellent biocompatible, antibacterial and anti-inflammatory properties based on quaternized cellulose (QC), sodium alginate (SA) and Zn was reported. The existence of physical interactions, such as electrostatic interaction, chelation and hydrogen bonding endowed the sponges with enhanced mechanical property. The composite sponges exhibited outstanding biocompatibility and hemostatic efficiency due to the compatible nature of the component and physical cross-linking, as well as superior antibacterial property benefited from the synergistic effects of steady Zn release and quaternary ammonium group. In vivo investigation validated that the enhanced antibacterial and anti-inflammatory effect of the sponges, which significantly promoted wound closure and the reconstruction of skin tissue through epithelial regeneration, collagen deposition and mitigating inflammatory cell infiltration. Overall, the novel sponge demonstrated great potentials in bacteria-associated wound management.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2021.09.047DOI Listing
September 2021

Shock in China 2018 (SIC-study): a cross-sectional survey.

Ann Transl Med 2021 Aug;9(15):1219

Department of Cardiology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, China.

Background: Shock is a critical illness that seriously threatens the lives of patients. This study explains the epidemiology of shock, mortality of shock, and identify factors that related to hospital death.

Methods: This is a multi-centre cross-sectional survey, which included 1,064 tertiary hospitals in 31 provinces, municipalities, and autonomous regions across China mainland. Totally 289,428 patients who diagnosed with shock based on the ICD-10 abstracted from the Hospital Quality Monitoring System (HQMS) in 2018, a national database administrated by National Health Commission of the PRC.

Results: Patients diagnosed with shock were screened and classified according to the type of shock. Regression analysis was used to identify factors that related to death. A total of 79,668,156 medical records were included in HQMS in 2018, from which a total of 289,428 records with shock were identified. Hypovolemic shock occurred in 128,436 cases (44.38%), septic shock occurred in 121,543 cases (41.99%), cardiogenic shock occurred in 44,597 cases (15.41), and obstructive shock occurred in 3,168 cases (1.09%). Of these, 8,147 cases (2.81%) had mixed shock, which means had two or more types of shock. For all the shock cases, the top three frequent concomitant diseases recorded were circulatory system diseases (55.22%), digestive system diseases (53.64%), and respiratory system diseases (53.31%). Of the four types of shock, cases with cardiogenic shock had the highest in-hospital mortality (31.6%), followed by those with obstructive shock (25.2%), septic shock (22.9%), and hypovolemic shock (15.5%). Interestingly, the combination of shock and malignant tumors is one of the major factors that related to hospital deaths.

Conclusions: Shock is a serious disease with a high fatality rate and huge clinical costs. According to this epidemiological survey of shock in China 2018, we should clarify the factors related to the hospital death in shock cases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-21-310DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421935PMC
August 2021

Association between maternal urinary selenium during pregnancy and newborn telomere length: results from a birth cohort study.

Eur J Clin Nutr 2021 Sep 16. Epub 2021 Sep 16.

Department of Maternal and Child Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Background: Newborn telomere length is considered as an effective predictor of lifespan and health outcomes in later life. Selenium is an essential trace element for human health, and its antioxidation is of great significance for the prevention of telomere erosion.

Methods: We recruited 746 mother-newborn pairs in Wuhan Children's Hospital between 2013 and 2015. Urine samples were repeatedly collected at three time points during pregnancy, and umbilical cord blood samples were collected right after parturition. Urinary selenium concentration was detected using inductively coupled plasma mass spectrometry, and newborn telomere length was measured using quantitative real-time polymerase chain reaction. We applied general estimating equations to examine the trimester-specific association between maternal urinary selenium during pregnancy and newborn telomere length.

Results: The median of creatinine-corrected selenium concentrations during pregnancy were 16.29, 18.08, and 18.35 μg/g·creatinine in the first, second, and third trimesters, respectively. Selenium concentrations in all the three trimesters were significantly associated with newborn telomere length. Per doubling of maternal urinary selenium concentrations was associated with 6.44% (95% CI: 0.92, 12.25), 6.54% (95% CI: 0.17, 13.31), and 6.02% (95% CI: 0.29, 12.09) longer newborn telomere length in the first, second, and third trimesters, respectively, after adjusting for potential confounders.

Conclusions: This is the first study to provide evidence for the effect of maternal selenium levels on fetal telomere erosion. Findings from our study suggested that maternal urinary selenium was positively associated with newborn telomere length, indicating that intrauterine selenium exposure might have effect on initial setting of human telomere length.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41430-021-01004-2DOI Listing
September 2021

Data Quality Management in the Internet of Things.

Sensors (Basel) 2021 Aug 30;21(17). Epub 2021 Aug 30.

Department of Software Convergence Engineering, Kunsan National University, Gunsan 54150, Korea.

Nowadays, IoT is being used in more and more application areas and the importance of IoT data quality is widely recognized by practitioners and researchers. The requirements for data and its quality vary from application to application or organization in different contexts. Many methodologies and frameworks include techniques for defining, assessing, and improving data quality. However, due to the diversity of requirements, it can be a challenge to choose the appropriate technique for the IoT system. This paper surveys data quality frameworks and methodologies for IoT data, and related international standards, comparing them in terms of data types, data quality definitions, dimensions and metrics, and the choice of assessment dimensions. The survey is intended to help narrow down the possible choices of IoT data quality management technique.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s21175834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434542PMC
August 2021

Highly Dispersed Pd Clusters Anchored on Nanoporous Cellulose Microspheres as a Highly Efficient Catalyst for the Suzuki Coupling Reaction.

ACS Appl Mater Interfaces 2021 Sep 8. Epub 2021 Sep 8.

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.

With the depletion of nonrenewable resources such as oil/coal/gas, more and more research studies began to focus on the high-value utilization of residual biomass resources. Herein, for the first time, honeycomb nanoporous microspheres fabricated from renewable biomass resources of cellulose were used as a carrier to fabricate a highly dispersed palladium (Pd) nanocatalyst. Various physicochemical characterizations presented convincing pieces of evidence for the good dispersion of Pd clusters with a mean diameter of 1.6 nm. As the carrier, cellulose microspheres with an interconnected nanoporous structure contributed to the adhesion and dispersion of Pd particles, and their rich hydroxyl groups could fix the Pd particles. Importantly, the cellulose matrix could induce the formation of metallic Pd(0) during calcination without a reductant. The cellulose/Pd catalyst was applied to the Suzuki coupling reaction, which exhibited promising catalytic activity compared to commercial Pd/C and unsupported homogeneous Pd(OAc) catalysts, as well as good stability. The utilization of the residual biomass resource to build catalyst materials would be important for the sustainable chemistry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c12850DOI Listing
September 2021

Application of a Cloud Video Conference Method for Recruiting Healthy Subjects Into an Early-Phase Clinical Trial During the COVID-19 Pandemic.

Front Public Health 2021;9:657804. Epub 2021 Aug 19.

Department of Clinical Pharmacology, Aerospace Center Hospital, Beijing, China.

Our objective is to explore the effect of applying cloud video conferencing methods to the informed consent process in an early-phase clinical trial during the COVID-19 pandemic. All participants who intended to participate in the trial were informed via a cloud video conference before signing the informed consent forms (ICF). Then, the attitudes of the participants with the cloud video conference and their understanding of the trial were evaluated using a questionnaire when they visited to sign the ICF onsite. A total of 165 subjects participated in the cloud video conference process, and 142 visited the site to sign and date the ICFs at the center during the appointment time. The survey showed that nearly 100% of the subjects evaluated the video-based informed consent process as very good or good and gave correct answers to questions about the trial. Furthermore, 136 (95.8%) subjects believed that the knowledge about the trial derived via the video-based informed consent process was consistent with the onsite reality, and 139 (97.9%) subjects expressed their willingness to participate in an informed consent procedure undertaken through an online video conference. The video-based informed consent process achieved the same effects as an onsite informed consent process. The former saves time and cost of transportation for the subject and exhibits good public acceptance; especially in light of the COVID-19 pandemic, this process is conducive for reducing the risk of subject infection due to travel and would also help avoid crowding on site.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpubh.2021.657804DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416603PMC
September 2021

Long Non-coding RNA N1LR Protects Against Myocardial Ischemic/Reperfusion Injury Through Regulating the TGF-β Signaling Pathway.

Front Cardiovasc Med 2021 13;8:654969. Epub 2021 Aug 13.

Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.

Long non-coding RNAs (lncRNAs) have been shown to play critical roles in various cell biological processes. However, the mechanism of lncRNAs in acute myocardial infarction (AMI) is not fully understood. Previous studies showed that lncRNA N1LR was down-regulated in ischemic cerebral stroke and its up-regulation was protective. The current study was designed to assess the protective effect of N1LR and further to explore potential mechanisms of N1LR in ischemic/reperfusion (I/R) injury after AMI. Male C57BL/6J mice and H9c2 cardiomyocytes were selected to construct and pathological models. In H9c2 cell line, N1LR expression was markedly decreased after HO and CoCl treatments and N1LR overexpression alleviated apoptosis, inflammation reaction, and LDH release in cardiomyocytes treated with HO and CoCl. Mouse study showed that overexpression of N1LR enhanced cardiac function and suppressed inflammatory response and fibrosis. Mechanistically, we found that the expression of transforming growth factor (TGF)-β1 and smads were significantly decreased in the N1LR overexpression group exposed to HO. In a summary, our study indicated that N1LR can act as a protective factor against cardiac ischemic-reperfusion injury through regulating the TGF-β/Smads signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcvm.2021.654969DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414635PMC
August 2021

Gestational Diabetes Mellitus-Induced Changes in Proteomes and Glycated/Glycosylated Proteomes of Human Colostrum.

J Agric Food Chem 2021 Sep 2;69(36):10749-10759. Epub 2021 Sep 2.

State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi, Jiangsu Province 214122, China.

Gestational diabetes mellitus (GDM) not only has a bad effect on the development of infants but also causes variations in breastmilk composition. This study aims to investigate the changes in the protein profile of colostrum between mothers with GDM and healthy mothers (H) by sequential windowed acquisition of all theoretical fragment ion proteomics techniques. A total of 1295 proteins were detected, with 192 proteins being significantly different between GDM and H. These significantly different proteins were enriched with the carbohydrate and lipid metabolism pathway as well as immunity. Some proteins had an AOC value of 1, such as apolipoprotein E and lipoprotein lipase. In addition, we identified 42 glycated and 93 glycosylated peptides in colostrum without any enrichment, with glycated peptides being upregulated and glycosylated peptides being downregulated in colostrum with GDM. These results help us to better understand the GDM-induced changes in proteomes and glycated and glycosylated level and provide guidance on infant formula adjustment for infants from mothers with GDM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.1c03791DOI Listing
September 2021

Comprehensive meta-analysis of anti-BCMA chimeric antigen receptor T-cell therapy in relapsed or refractory multiple myeloma.

Ann Med 2021 12;53(1):1547-1559

Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing, China.

Background: Chimeric antigen receptor (CAR) T-cell therapy shows impressive results in clinical trials. We conducted a meta-analysis based on the most recent data to systematically describe the efficacy and safety of anti-BCMA CAR T therapy for patients with relapsed or refractory multiple myeloma (R/R MM).

Methods: PubMed, Embase, Web of Science, Cochrane library, ClinicalTrials.gov, China Biology Medicine disc (CBM disc) and Wanfang Data were searched on 8 November 2020. Registration number of PROSPERO was CRD42020219127.

Results: From 763 articles, we identified 22 appropriate studies with 681 patients. The pooled overall response rate (ORR) was 85.2% (95%CI 0.797-0.910), complete response rate (CRR) was 47.0% (95%CI 0.378-0.583), and minimal residual disease (MRD) negativity rate was 97.8% (95%CI 0.935-1.022). The pooled incidence of grade 3-4 cytokine release syndrome was 6.6% (95%CI 0.036-0.096) and neurotoxicity was 2.2% (95%CI 0.006-0.038). The median progression-free survival (PFS) was 14.0 months and median overall survival (OS) was 24.0 months. Subgroup analysis showed dual epitope-binding CAR T cells achieved the best therapy outcomes and humanized CAR T cells had the best safety profile. Patients who were older, heavily pre-treated or received lower dose of CAR T cells had worse ORR. There was no significant difference in ORR, CRR and PFS between patients with and without high-risk cytogenetic features. The PFS and CRR of non-extramedullary disease (EMD) group was superior to those of EMD group.

Conclusion: Anti-BCMA CAR T therapy is effective and safe for patients with R/R MM. It can improve the prognosis of patients with high-risk cytogenetic features while the prognosis of patients with EMD remains poor. Moreover, patients are likely to benefit from an earlier use of CAR T therapy and human-derived CAR T cells have obvious advantages based on the existing data.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/07853890.2021.1970218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8409966PMC
December 2021

Role of aneuploid circulating tumor cells and CD31 circulating tumor endothelial cells in predicting and monitoring anti-angiogenic therapy efficacy in advanced NSCLC.

Mol Oncol 2021 Aug 28. Epub 2021 Aug 28.

Department of Medical Oncology, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.

Prognosticating the efficacy of anti-angiogenic therapy through longitudinal monitoring and early detection of treatment resistance in cancer patients remain highly challenging. In this study, co-detection and comprehensive phenotypic and karyotypic molecular characterization of aneuploid circulating tumor cells (CTCs) and circulating tumor endothelial cells (CTECs) were conducted on non-small cell lung cancer (NSCLC) patients receiving bevacizumab plus chemotherapy. Prognostic values of the cell-based significant univariate risk factors identified by Cox regression analyses were progressively investigated. Subjects showing an increase in total post-therapeutic platelet endothelial cell adhesion molecule-1 (CD31) CTCs and CD31 CTECs exhibited a significantly reduced median progression-free survival (mPFS) and overall survival. Further stratification analyses indicated that pretherapeutic patients bearing vimentin (Vim) CTECs (mesenchymal M-type) at baseline revealed a significantly shortened mPFS compared with patients with Vim CTECs. Post-therapeutic patients harboring epithelial cell adhesion molecule (EpCAM) CTCs and CTECs (epithelial E-type), regardless of Vim expression or not, showed a significantly reduced mPFS. Post-therapeutic patients possessing de novo EpCAM /Vim (hybrid E/M-type) CTECs displayed the shortest mPFS. Patients harboring either pre- or post-therapeutic EpCAM /Vim null CTECs (N-type) exhibited a better response to therapy compared to patients harboring EpCAM and/or Vim CTECs. The presented results support the notion that baseline Vim CTECs and post-therapeutic EpCAM CTCs and CTECs are predictive biomarkers for longitudinal monitoring of response to anti-angiogenesis combination regimens in NSCLC patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/1878-0261.13092DOI Listing
August 2021

Indicators of Glucose Dysregulation and the Relationship with Iron Overload in Chinese Children with Beta Thalassemia Major.

Pediatr Diabetes 2021 Aug 28. Epub 2021 Aug 28.

Department of Pediatrics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Introduction: Patients with beta thalassemia major (TM) have a higher risk of diabetes and an abnormal oral glucose tolerance test (OGTT), but there is no single agree monitoring parameter that reflects glycemic status. The possible mechanisms include iron overload and blood transfusion, but they require further investigation.

Purpose: This study explored the role of glycated hemoglobin A1c (HbA1c), fructosamine, and glycated albumin (GA) in evaluating the glucose dysregulation and to determine the potential relationship between iron deposition and glucose metabolism disorder in beta TM.

Methods: A cross-sectional study was performed on 118 patients with beta TM and the control group consisted of 33 healthy children with no statistical differences in age, sex, and body mass index (BMI). Fast plasma glucose (FPG), fast insulin (FINS), insulin resistance index (HOMA-IRI), and insulin sensitivity index (HOMA-ISI) were compared between the patient and control groups. HbA1c, GA, fructosamine, and serum ferritin (SF) were measured in the patient group. OGTT, as well as heart and liver magnetic resonance imaging (MRI) T2*, was performed. For all statistical analyses, SPSS 21.0 was used and P<0.05 was accepted as statistically significant RESULTS: FPG, FINS, and HOMA-IRI were significantly increased while HOMA-ISI decreased in the beta TM patients when compared with those in the control group. In patients with beta TM, 17 (14.41%) of patients had been diagnosed with diabetes, while 48 (40.68%) had both impaired fasting glucose (IFG) and glucose tolerance (IGT). HbA1c, GA, and fructosamine were increased according to the degree of abnormal glucose metabolism. Statistically significant differences were found in age, serum ferritin, and cardiac T2* between the abnormal and normal OGTT groups.

Conclusion: HbA1c may be used as a significant measure for monitoring glycemic levels in patients with beta TM. Furthermore, glycated albumin and fructosamine were alternative indicators of glucose status. Patients with heart iron deposition or an SF > 4000 μg/L were prone to abnormal glucose metabolism, so chelation therapy should be reinforced. This article is protected by copyright. All rights reserved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/pedi.13260DOI Listing
August 2021

Continuous Meter-Scale Wet-Spinning of Cornlike Composite Fibers for Eco-Friendly Multifunctional Electronics.

ACS Appl Mater Interfaces 2021 Sep 18;13(34):40953-40963. Epub 2021 Aug 18.

College of Chemistry and Molecular Sciences, Hubei Engineering Center of Natural Polymer-Based Medical Materials, Wuhan University, Wuhan 430072, China.

"Green" solvent-dissolved cellulose enables functional reuse of waste cotton fabrics. This work will not only achieve high-value utilization of biomass but also overcome microplastic pollution. There is a significant challenge in the continuous meter-scale synthesis of sensing fibers for commercial applications with high productivity. Herein, waste cellulose fabrics was recycled by the NaOH/urea system to produce regenerated cellulose (RC) and then cornlike polyaniline (PANI) was anchored on the RC fibers by polymerization of aniline through continuous meter-scale wet-spinning. In our findings, the morphologies and possible growth of PANI layers on the RC surface can be tailored by various ammonium persulfate (APS) contents in a coagulation bath. Especially, composite fibers (PC) exhibited superior electrical conductivity and highly sensitive responsiveness to organic vapors and human motions including exhalation/inhalation, finger, and wrist joints. Further, the possible sensing mechanism of cornlike PC has been proposed, and its GF value is 23.8. This study realized the conversion from cheap waste fibers to high-value conductive fibers with excellent performances for multifunctional wearable sensors and energy devices via a simple and "green" method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c12012DOI Listing
September 2021

Advances and Prospects in Metal-Organic Frameworks as Key Nexus for Chemocatalytic Hydrogen Production.

Small 2021 Aug 15:e2102201. Epub 2021 Aug 15.

Institute of Chemical Industry of Forest Products, CAF, National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, 210042, P. R. China.

Hydrogen is a clean and sustainable energy carrier, which is considered a promising alternative for fossil fuels to solve the global energy crisis and respond to climate change. Social concerns on its safe storage promote continuous exploration of alternatives to traditional storage methods. In this case, chemical hydrogen storage materials initiate plentiful research with special attention to the design of heterogeneous catalysts that can enhance efficient and highly selective hydrogen production. Metal-organic frameworks (MOFs), a kind of unique crystalline porous materials featuring highly ordered porosities and tailorable structures, can provide various active sites (i.e., metal nodes, functional linkers, and defects) for heterogeneous catalysis. Furthermore, the easy construction of active sites in highly ordered MOFs, which can work as plate for the delicate active site engineering, make them ideal candidates for a variety of heterogeneous catalysts including chemocatalytic hydrogen production. This review concentrates on the application of MOFs as heterogeneous catalysts or catalyst supports in chemocatalytic hydrogen production. Recent progresses of MOFs as catalysts for chemocatalytic hydrogen production are comprehensively summarized. The research methods, mechanism analyses, and prospects of MOFs in this field are discussed. The challenges in future industrial applications of MOFs as catalysts for hydrogen production are proposed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202102201DOI Listing
August 2021

Probiotics use is associated with improved clinical outcomes among hospitalized patients with COVID-19.

Therap Adv Gastroenterol 2021 4;14:17562848211035670. Epub 2021 Aug 4.

Department of Respiratory and Critical Care Medicine, Affiliated Hospital, Institute of Respiratory Diseases, Guangdong Medical University, Zhanjiang, Guangdong, China.

Background And Aims: Currently, there are no definitive therapies for coronavirus disease 2019 (COVID-19). Gut microbial dysbiosis has been proved to be associated with COVID-19 severity and probiotics is an adjunctive therapy for COIVD-19. However, the potential benefit of probiotics in COVID-19 has not been studied. We aimed to assess the relationship of probiotics use with clinical outcomes in patients with COVID-19.

Methods: We conducted a propensity-score matched retrospective cohort study of adult patients with COVID-19. Eligible patients received either probiotics plus standard care (probiotics group) or standard care alone (non-probiotics group). The primary outcome was the clinical improvement rate, which was compared among propensity-score matched groups and in the unmatched cohort. Secondary outcomes included the duration of viral shedding, fever, and hospital stay.

Results: Among the propensity-score matched groups, probiotics use was related to clinical improvement rates (log-rank  = 0.028). This relationship was driven primarily by a shorter (days) time to clinical improvement [difference, -3 (-4 to -1),  = 0.022], reduction in duration of fever [-1.0 (-2.0 to 0.0),  = 0.025], viral shedding [-3 (-6 to -1),  < 0.001], and hospital stay [-3 (-5 to -1),  = 0.009]. Using the Cox model with time-varying exposure, use of probiotics remained independently related to better clinical improvement rate in the unmatched cohort.

Conclusion: Our study suggested that probiotics use was related to improved clinical outcomes in patients with COVID-19. Further studies are required to validate the effect of probiotics in combating the COVID-19 pandemic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/17562848211035670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351028PMC
August 2021

Treatment Outcomes of Clopidogrel in Patients With ACS and Diabetes Undergoing PCI-Analysis of Beijing Municipal Medical Insurance Database.

Front Endocrinol (Lausanne) 2021 30;12:713849. Epub 2021 Jul 30.

Department of Endocrinology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China.

Background And Aims: Several clinical trials have proved the efficacy of clopidogrel treatment for patients with percutaneous coronary intervention. There are few large-scale studies to identify the mortality associated with different durations of treatment of clopidogrel in patients with diabetes and ACS undergoing PCI in the Chinese population. The objective of this analysis was to determine the efficacy of long-term clopidogrel therapy (≥12 months) short-term use (<12 months) in Chinese patients with diabetes after PCI.

Methods And Results: We used the Beijing Municipal Medical Insurance Database provided by the Beijing Municipal Medical Insurance Bureau. The Beijing Municipal Medical Insurance Database contained medical data of about 16 million people, including about 990,000 patients with diabetes and a history of taking antidiabetic medicines. Patients were divided into two groups, one group of 9,116 patients receiving consecutive clopidogrel for one year or more, and another group of 3290 patients receiving consecutive clopidogrel for less than one year. The primary outcomes of this analysis were the risk of all-cause death, myocardial infarction, and revascularization. In patients with diabetes after PCI, long-term clopidogrel treatment was associated with a reduced risk of all-cause death (HR, 0.57[95%CI, 0.49-0.67], P<0.0001), myocardial infarction (HR, 0.79[95%CI, 0.68-0.93], P=0.0035) and an increased risk of angina (HR, 1.18[95%CI, 1.10-1.27], P<0.0001]) and revascularization (HR, 1.07[95%CI, 1.01-1.13], P=0.02]). There was no significant difference in the prevalence of all-cause re-hospitalization, diabetes-related re-hospitalization, and cerebrovascular re-hospitalization.

Conclusion: The present study concluded that long-term dual antiplatelet therapy including clopidogrel and aspirin could decrease the risks of all-cause death, myocardial infarction. But it could increase the risks of angina and revascularization. Further studies should interpret the cause of this question.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fendo.2021.713849DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8362599PMC
July 2021

The potential role of RAAS-related hsa_circ_0122153 and hsa_circ_0025088 in essential hypertension.

Clin Exp Hypertens 2021 Aug 16:1-8. Epub 2021 Aug 16.

Insitute of Geriatrics, the Affiliated Hospital of Medical School, Ningbo University, Ningbo, Zhejiang Province, China.

: The dysregulation of renin-angiotensin-aldosterone system (RAAS) is closely related to the development of essential hypertension (EH). MicroRNAs (miRNAs) are an important regulator of RAAS. The sponge effect of circular RNAs (circRNAs) on miRNAs makes the circRNA-miRNA-mRNA axis in EH possible, however, there is currently a lack of relevant evidence.: A circRNA-miRNA network was constructed based on the previous circRNAs microarray results. The expression of RAAS-related miRNAs and circRNAs were verified by qRT-PCR. Peripheral blood samples of 106 EH patients and 106 healthy volunteers were included in this study. GO and KEGG enrichment were performed to predict the role of candidate circRNAs in EH.: In EH patients, RAAS-related hsa-miR-483-3p and hsa-miR-27a-3p were down-regulated, and hsa_circ_0122153 and hsa_circ_0025088 were up-regulated. The relative expression of RAAS-related circRNAs and target miRNAs showed a negative correlation (hsa_circ_0122153-hsa-miR-483-3p and hsa_circ_0025088-hsa-miR-27a-3p). Hsa_circ_0122153 or hsa_circ_0025088 combined with corresponding miRNAs and environmental factors may support the early diagnosis of EH. Hsa_circ_0122153 and hsa_circ_0025088 may participate in the regulation of aldosterone and the secretion of renin through the circRNA-miRNA-mRNA network, respectively.: Highly expressed hsa_circ_0122153 and hsa_circ_0025088 increase the risk of EH. The hsa_circ_0122153/hsa-miR-483-3p and hsa_circ_0025088/hsa-miR-27a-3p axis involving RAAS were potential EH pathways.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10641963.2021.1945077DOI Listing
August 2021

Quantitative Determination of -Cymene, Thymol, Neryl Acetate, and -Caryophyllene in Different Growth Periods and Parts of Turcz. by GC-MS/MS.

J Anal Methods Chem 2021 2;2021:2174667. Epub 2021 Aug 2.

Department of Anesthesiology and Operation, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.

Turcz. is a widely used Chinese herbal medicine in China. In this study, a gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) method was developed and validated to simultaneously determine the contents of -cymene, thymol, neryl acetate, and -caryophyllene in roots, stems, and leaves of Turcz. harvested at different growth periods. All four constituents could be detected in leaves, three could be detected in stems except -caryophyllene, and only thymol could be detected in roots. The order of the total contents of four constituents in different parts was leaves > stems > roots. It indicated that the leaves could be the proper medicinal parts of Turcz. The content of four constituents in leaves varied a lot among different growth periods and showed an M-shaped change trend with the growth of Turcz. The four constituents accumulated to the highest values in early July followed by mid-September. Accordingly, the best harvest time of Turcz. is early July and mid-September.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/2174667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352711PMC
August 2021

Human umbilical cord mesenchymal stem cells reduce oxidative damage and apoptosis in diabetic nephropathy by activating Nrf2.

Stem Cell Res Ther 2021 08 11;12(1):450. Epub 2021 Aug 11.

Department of Nephropathy, The Second Hospital of Jilin University, Changchun, Jilin Province, China.

Background: Mesenchymal stem cells (MSCs) have a therapeutic effect on diabetic nephropathy (DN) but the underlying mechanism remains unclear. This study was conducted to investigate whether human umbilical cord-MSCs (hUCMSCs) can induce oxidative damage and apoptosis by activating Nrf2.

Methods: We used a type 2 diabetic rat model and a high-glucose and fat-stimulated human glomerular mesangial cell (hGMC) model. Western blotting, RT-qPCR, and TUNEL staining were performed on animal tissues and cultured cells. Nuclear expression of Nrf2 was detected in the renal tissue. Furthermore, Nrf2 siRNA was used to examine the effects of hUCMSCs on hGMCs. Finally, the effect of hUCMSCs on the Nrf2 upstream signalling pathway was investigated.

Results: After treatment with hUCMSCs, Nrf2 showed increased expression and nuclear translocation. After Nrf2-specific knockout in hGMCs, the protective effect of hUCMSCs on apoptosis induced by high-glucose and fat conditions was reduced. Activation of the PI3K signalling pathway may be helpful for ameliorating DN using hUCMSCs.

Conclusions: hUCMSCs attenuated renal oxidative damage and apoptosis in type 2 diabetes mellitus and Nrf2 activation is one of the important mechanisms of this effect. hUCMSCs show potential as drug targets for DN.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13287-021-02447-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8356418PMC
August 2021

Using a new HSPC senescence model in vitro to explore the mechanism of cellular memory in aging HSPCs.

Stem Cell Res Ther 2021 08 9;12(1):444. Epub 2021 Aug 9.

Institute of Basic Medicine, Shanghai University of Traditional Chinese Medicine, 1200 CaiLun Ave., Pudong, Shanghai, 201203, China.

Background: Age-associated changes attenuate human blood system functionality through the aging of hematopoietic stem and progenitor cells (HSPCs), manifested in human populations an increase in myeloproliferative disease and even leukemia; therefore, study on HSPC senescence bears great significance to treat hematopoietic-associated disease. Furthermore, the mechanism of HSPC aging is lacking, especially the cellular memory mechanism. Here, we not only reported a new HSPC senescence model in vitro, but also propose and verify the cellular memory mechanism of HSPC aging of the Polycomb/Trithorax system.

Methods: HSPCs (Linc-kit cells) were isolated and purified by magnetic cell sorting (MACS). The proportions and cell cycle distribution of cells were determined by flow cytometry; senescence-related β-galactosidase assay, transmission electron microscope (TEM), and colony-forming unit (CFU)-mix assay were detected for identification of the old HSPC model. Proteomic tests and RNA-seq were applied to analyze differential pathways and genes in the model cells. qPCR, Western blot (WB), and chromatin immunoprecipitation PCR (CHIP-PCR) were used to detect the gene expression of cell memory-related proteins. Knockdown of cell memory-related key genes was performed with shRNA interference.

Results: In the model old HSPCs, β-gal activity, cell cycle, colony-forming ability, aging-related cell morphology, and metabolic pathway were significantly changed compared to the young HSPCs. Furthermore, we found the model HSPCs have more obvious aging manifestations than those of natural mice, and IL3 is the major factor contributing to HSPC aging in the model. We also observed dramatic changes in the expression level of PRC/TrxG complexes. After further exploring the downstream molecules of PRC/TrxG complexes, we found that Uhrf1 and TopII played critical roles in HSPC aging based on the HSPC senescence model.

Conclusions: These findings proposed a new HSPC senescence model in vitro which we forecasted could be used to preliminary screen the drugs of the HSPC aging-related hemopathy and suggested cellular memory mechanism of HSPC aging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13287-021-02455-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351417PMC
August 2021

Measuring urban integrated water use efficiency and spatial migration path in China: A dynamic two-stage recycling model within the directional distance function.

J Environ Manage 2021 Aug 3;298:113379. Epub 2021 Aug 3.

Business School, Hohai University, Changzhou, 213022, China. Electronic address:

Improving water use efficiency from a recycling perspective is claimed to be a better way to ensure prosperity in a water-stressed world. Although many studies have focused on the efficiency of integrated water use system, such as a water use and wastewater treatment (WUWT) system, few studies have observed the dynamic change involving a two-stage recycling structure. This research thus proposes a dynamic two-stage recycling model within the directional distance function (DDF) by taking reused water in the WT stage as a recycled product for WU stage's input in the next period. This paper reveals the dynamic evolution and spatial migration path of this efficiency in China. Results are as follows. (1) The average overall efficiency of the urban WUWT system for 30 provinces during 2011-2018 was 0.78. The central region's lower WUWT efficiency was caused by the expanding provincial differences in WU efficiency. (2) The urban WU sub-system performed poorer than that of the urban WT sub-system due to underutilizing reused water, such as in Jiangxi, Guangxi, Yunnan, and Jilin. (3) Narrowing the spatial imbalance of economic development and water use between the eastern and western regions is of great importance for promoting China's spatial imbalance of urban WUWT efficiency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jenvman.2021.113379DOI Listing
August 2021

Comparison of milk fat globule membrane and whey proteome between Dromedary and Bactrian camel.

Food Chem 2021 Jul 22;367:130658. Epub 2021 Jul 22.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Electronic address:

Camel milk is rich in nutrients and its impact on human medicine and nutrition cannot be ignored. We conducted an in-depth analysis of milk proteins obtained from two camel breed (Camelus bactrianus, CB and Camelus dromedarius, CD). Label-free proteomic technology was performed to analysis the MFGM and whey proteomes of CB and CD milk. In total, 1133 MFGM proteins and 627 whey proteins were identified from camel milk. Results revealed that 216 MFGM proteins and 109 whey proteins were significantly different between them. In addition, the cellular process, cell and binding were the predominately GO annotations of milk proteins. KEGG analysis shown that most proteins were involved in metabolic pathways. Furthermore, many proteins were found to be involved in PI3K/AKT signaling pathway, which could be the possible reason for hypoglycemic effect of camel milk. These results could provide a further understanding for unique biological characteristics of camel milk proteins.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2021.130658DOI Listing
July 2021

Stabilizing the framework of SAPO-34 zeolite toward long-term methanol-to-olefins conversion.

Nat Commun 2021 Aug 2;12(1):4661. Epub 2021 Aug 2.

School of Materials Science and Engineering, and National Institute for Advanced Materials, Nankai University, Tianjin, P.R. China.

As a commercial MTO catalyst, SAPO-34 zeolite exhibits excellent recyclability probably due to its intrinsic good hydrothermal stability. However, the structural dynamic changes of SAPO-34 catalyst induced by hydrocarbon pool (HP) species and the water formed during the MTO conversion as well as its long-term stability after continuous regenerations are rarely investigated and poorly understood. Herein, the dynamic changes of SAPO-34 framework during the MTO conversion were identified by 1D Al, P MAS NMR, and 2D P-Al HETCOR NMR spectroscopy. The breakage of T-O-T bonds in SAPO-34 catalyst during long-term continuous regenerations in the MTO conversion could be efficiently suppressed by pre-coking. The combination of catalyst pre-coking and water co-feeding is established to be an efficient strategy to promote the catalytic efficiency and long-term stability of SAPO-34 catalysts in the commercial MTO processes, also sheds light on the development of other high stable zeolite catalyst in the commercial catalysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-24403-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329068PMC
August 2021

All-sealed paper-based electrochemiluminescence platform for on-site determination of lead ions.

Biosens Bioelectron 2021 Jul 21;192:113524. Epub 2021 Jul 21.

School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China. Electronic address:

Lab-on-paper (LOP) devices are urgently required for the rapid development of point-of-care diagnoses and environmental assays. Herein, an all-sealed paper-based electrochemiluminescence (ECL) platform was developed to achieve lead ions (Pb) sensitive analysis via incorporating convenient plastic package technology. Benefiting from transparent plastic encapsulation, the sealed devices effectively avoided the interference of O. Meanwhile, myrica rubra-like Pt nanomaterials (MPNs) prepared by an economical and easy-to-operate ultrasound method were employed to catalyze HO decomposition. With the help of Pb-specific DNAzymes, the oligonucleotide probe functionalized via MPNs could be detached from the device in the presence of target, resulting in the reduced ECL intensity. Moreover, the combination of modified paper electrode with functional regions separated by multiple layers of wax enhanced the practicability of the LOP device for rapid detection. Under the optimal conditions, the all-sealed platform achieved wide linear relationship ranging from 0.01 nM to 0.05 μM with a low detection limit of 0.004 nM for sensitive detecting Pb. It is believed that this platform could provide a robust, simple and versatile strategy for sensitive determination of heavy metal ions, and be applied in on-site contamination analysis in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2021.113524DOI Listing
July 2021

[Relationship between nutritional status and breakfast behaviors of rural primary and middle school students in a minority area of a city in Guizhou Province in 2019].

Wei Sheng Yan Jiu 2021 Jul;50(4):552-557

School of Public Health, Guizhou Medical University, Guiyang 550004, China.

Objective: To investigate the nutritional status and influencing factors of rural primary and middle school students in a minority area in a certain city of Guizhou Province, so as to provide scientific evidence for prevention and intervention.

Methods: A multi-stage stratified cluster random sampling method was adopted in this study, 3952 students from 6 primary and middle schools in a certain city of Guizhou Province were selected from April to June 2019 for height and weight measurement(1966 boys, 1986 girls, age(10.81±2.88) years old), among which 2833 students in grade 4 and above were surveyed(1374 boys and 1459 girls, age(13.44±1.94) years old), through the questionnaire to understand students& apos; basic information, breakfast and eating behaviors and so on, Using multi-factor Logistic regression to analyze the relationship between nutritional status and breakfast behavior.

Results: The height and weight of Han male students and female students in all age groups were slightly higher than those of ethnic minorities, but they tended to overlap gradually by the age of 16.The general height and weight of students in this area were lower than the 2014 national average. The general malnutrition rate of students was 21.39%, and the growth retardation rate was 13.13%, the moderate-to-severe wasting rate 3.04% and the mild wasting rate 5.22%. The malnutrition rate of minority students(24.72%) was higher than that of Han students(18.73%)(χ~2=14.93, P& lt; 0.05), the malnutrition rate of boys(26.49%) was higher than that of girls(16.59%)(χ~2=41.29, P& lt; 0.05). The sum of the rate of overweight and obesity of the students was 8.65%, among which the overweight rate was 6.42% and the obesity rate was 2.22%. There was no statistically significant difference in the variables between overweight and obese students(P& gt; 0.05). The breakfast behavior situation showed that rice noodle products(71.66%) were the most frequently consumed by primary and middle school students in the city. Multivariate Logistic regression analysis showed that the frequency of breakfast, the quality of breakfast, fresh vegetables, milk and products, and the frequency of meat/egg/fish were the main influencing factors of malnutrition. The differences were statistically significant(P& lt; 0.05).

Conclusion: The growth and development status of primary and middle school students in a city of Guizhou Province mainly includes malnutrition, and the rate of overweight and obesity is not high, but prevention and control should still be paid attention to. The frequency and quality of breakfast are important influencing factors of malnutrition.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2021.04.004DOI Listing
July 2021

The Distributional Characteristics of Multiple Sclerosis Lesions on Quantitative Susceptibility Mapping and Their Correlation With Clinical Severity.

Front Neurol 2021 9;12:647519. Epub 2021 Jul 9.

Department of Radiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Multiple sclerosis (MS) patients have a wide spectrum of severity and responses to therapy; the personalization of treatment relies on sensitive and specific biomarkers. Previous studies have suggested that susceptibility contrast in demyelinated plaques is associated with iron-related pathology in multiple sclerosis which may indicate clinical severity. The aims of this study were to characterize the spatial distribution of MS lesions with different iron patterns by using quantitative susceptibility mapping and to explore neuroradiological findings that correlate with poor clinical outcome. Twenty-six patients with relapsing-remitting MS [14 men, 12 women; mean age, 29 ± 8 (standard deviation) years; age range, 21-52 years] were included in this study. Differences in lesion number, T2 volume, and susceptibility were compared among lesions subcategorized by location and by the presence or absence of a hyperintense rim on quantitative susceptibility mapping. Associations between these imaging features and clinical outcomes including Expanded Disability Status Scale scores and annual relapse rates were investigated. A total of 811 unifocal MS lesions were included, and their QSM patterns were nodular hyperintensity with no rim (rim-, 540, 67%) or with a hyperintense rim on the edge (rim+, 172, 21%) and nodular isointensity (99, 12%). Rim+ lesions had significantly larger volume (115 ± 142 vs. 166 ± 185 mm, < 0.001) and lower susceptibility (4 ± 15 vs. 8 ± 16 ppb, < 0.05) than rim- lesions. More rim+ lesions were found in periventricular areas [median, 45%; interquartile range (IQR), 36%], whereas a larger proportion of rim- lesions were distributed in juxtacortical (median, 32%; IQR, 21%) and deep white matter (median, 38%; IQR, 22%) areas. The annual relapse rate was positively correlated with the proportion of periventricular rim+ lesions ( < 0.001, = 0.65) and the proportion of subtentorial rim+ lesions ( < 0.05, = 0.40). Additionally, a significant association was found between the burden of periventricular rim+ lesions (β = 0.64, < 0.001) and the burden of subtentorial rim- lesions (β = 0.36, < 0.05). A high number or lesion burden of periventricular rim+ lesions or subtentorial lesions is associated with frequent clinical relapses.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2021.647519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299522PMC
July 2021

Application of ultra pulse CO lattice laser in the treatment of female urinary incontinence.

Transl Androl Urol 2021 Jun;10(6):2471-2477

Center for Diagnosis and Treatment of Cervical Diseases, Changzhou Maternal and Child Health Care Hospital affiliated to Nanjing Medical University, Changzhou, China.

Background: This study aimed to explore the application of CO lattice laser in the treatment of mild and moderate urinary incontinence in female patients.

Methods: We analyzed the clinical data of 33 female patients with mild to moderate urinary incontinence from a prospective cohort study, who were treated with FemiLift CO lattice laser instrument (Alma Laser Ltd, Caesarea, Israel) at our hospital from January 2018 to December 2018. All the female patients were married and fertile, with a mean age of (43.15±6.49) years old (35-62 years old). There were 18 participants with mild urinary incontinence and 15 with moderate urinary incontinence. All participants received laser treatment 3 times, with an interval of 30 days. The International Consultation of Incontinence Questionnaire- Short Form (ICIQ-SF) survey was conducted before the treatment, 3 months after, and 6 months after the treatment. A 1-hour urine pad test was used to quantify the leakage of urine. Residual urine and urine routine examination were performed to exclude other combined diseases.

Results: After 3 times of treatment with FemiLift CO laser, 13 cases were cured in 3 months, and 16 cases were cured in 6 months. 10 cases and 15 cases were significantly improved in 3 or 6 months, respectively. 2 patients had no significant changes, and no adverse reactions were recorded. The pad test showed that the symptom of all 33 patients were significantly improved, and the average scores of ICIQ-SF were significantly decreased (P<0.05) from 12.36 (Grade I) and 15.67 (Grade II) (5-21) before treatment to 3.73(Grade I) and 5.49 (Grade II) (1-12) after 6 months of therapy.

Conclusions: The CO lattice laser is safe and effective in the treatment of mild and moderate urinary incontinences in female patients, which can effectively improve the symptom of urinary leakage without obvious adverse reactions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/tau-21-337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261411PMC
June 2021

Surface engineering of cellulose film with myristic acid for high strength, self-cleaning and biodegradable packaging materials.

Carbohydr Polym 2021 Oct 10;269:118315. Epub 2021 Jun 10.

College of Chemistry and Molecular Sciences, Engineering Research Center of Natural Polymer-based Medical Materials in Hubei Province, Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, China. Electronic address:

Developing sustainable, renewable, hydrophobic, and biodegradable packaging material to replace petroleum-based plastic products remains a challenge. Herein, original cellulose/myristic acid composite films were fabricated by solvent-vaporized controllable crystallization of natural myristic acid on anisotropic cellulose films. The myristic acid crystals that evenly distributed on the surface of cellulose film generated micronano binary structure and the interstitial space between microplates, resulting in high hydrophobicity (water contact angle = 132°) and excellent self-cleaning property of the composite film. The resultant film exhibited good tensile strength and toughness under both dry (188.7 MPa, 34.4 MJ m) and humid conditions (119.9 MPa, 28.7 MJ m). Moreover, these composite films could be degraded completely after approximately 102 days in soil with an average environment temperature of 32 °C. This work provided a low-cost and sustainable pathway for the fabrication of high-strength, self-cleaning, and waterproof packaging materials instead of plastics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.carbpol.2021.118315DOI Listing
October 2021

New insights into the anti- hepatoma mechanism of triple-helix β- glucan by metabolomics profiling.

Carbohydr Polym 2021 Oct 2;269:118289. Epub 2021 Jun 2.

State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong, China.

Natural polysaccharide as the third abundant biomacromolecule has attracted considerable attentions due to their superior anti-tumor activities. However, the anti-tumor mechanism of polysaccharides has not been completely understood. Herein, the anti-tumor effects of black fungus polysaccharide (BFP), a typical β-glucan was comprehensively investigated, and the anti-tumor mechanism was obtained from metabolomics profiling. The in vitro results demonstrate that BFP inhibited the proliferation, migration and invasion of hepatoma carcinoma cells (HCC) through inducing the cell apoptosis and arresting the cell cycle at S phase without direct cytotoxicity. The hepatoma-bearing nude mice experiments further demonstrate that BFP could significantly inhibit the growth without system toxicity in vivo. Mass spectrometry-based metabolomics unveils that BFP significantly disturbed the multiple metabolic pathways, leading to the inhibition of tumor cells proliferation by promoting DNA damage, attenuating DNA damage repair, and inhibiting DNA synthesis. This study provides new insights for pharmacological research and clinical practice of polysaccharides.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.carbpol.2021.118289DOI Listing
October 2021

Multifunctional chitin-based barrier membrane with antibacterial and osteogenic activities for the treatment of periodontal disease.

Carbohydr Polym 2021 Oct 1;269:118276. Epub 2021 Jun 1.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), Key Laboratory of Oral Biomedicine Ministry of Education, Department of Dental Implantology, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, PR China. Electronic address:

The guided tissue regeneration technique is an effective approach to repair periodontal defect. However, collagen barrier membranes used clinically lose stability easily, leading to soft tissue invasion, surgical site infection, and failure of osteogenesis. An ideal barrier membrane should possess proper antibacterial, osteoconductive activities, and favorable biodegradation. In this study, zinc oxide nanoparticles were homogeneously incorporated into the chitin hydrogel (ChT-1%ZnO) through one-step dissolution and regeneration method from alkaline/urea solution the first time. The remaining weights of ChT-1%ZnO in 150 μg/mL lysozyme solution was 52% after 5 weeks soaking. ChT-1%ZnO showed statistical antibacterial activities for P. gingivalis and S. aureus at 6 h, 12 h, and 24 h. Moreover, ChT-1%ZnO exhibits osteogenesis promotion in vitro, and it was further evaluated with rat periodontal defect model in vivo. The cemento-enamel junction value in ChT-1%ZnO group is 1.608 mm, presenting a statistical difference compared with no-membrane (1.825 mm) and ChT group (1.685 mm) after 8 weeks postoperatively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.carbpol.2021.118276DOI Listing
October 2021

Enhanced Catalytic Activity Induced by the Nanostructuring Effect in Pd Decoration onto Doped Ceria Enabling an Origami Paper Analytical Device for High Performance of Amyloid-β Bioassay.

ACS Appl Mater Interfaces 2021 Jul 19;13(29):33937-33947. Epub 2021 Jul 19.

School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, Shandong, P. R. China.

In this work, we fabricated a novel origami paper-based analytical device (oPAD) assisted by the nanostructuring effect of in situ Pd decoration of Cu/Co-doped CeO (CuCo-CeO-Pd) nanospheres, functionalized with their strongly enhanced electrocatalytic properties to realize an electrochemical and visual signal readout system in oPAD, for highly sensitive detection of amyloid-β (Aβ). The CuCo-CeO-Pd nanospheres were introduced as an enhanced "signal transducer layer" on account of the electron transfer acceleration caused by catalyzing glucose to produce HO for differential pulse voltammetry signal readout and further 3,3'5,5'-tetramethylbenzidine (TMB) oxidation for colorimetric analysis. Meanwhile, for achieving superior performance of the proposed oPAD, in situ growth of urchin-like gold nanoparticles (Au NPs) onto cellulose fibers was adopted to improve "the recognition layer" in favor of immobilizing antibodies for targeting Aβ through specific antigen-antibody interactions. Combined with the delicate design of oPAD, exhibiting actuation of the conversion procedure between hydrophobicity and hydrophilicity on paper tabs in the assay process, the oPAD successfully enabled sensitive diagnosis of Aβ in a linear range from 1.0 pM to 100 nM with a limit of detection of 0.05 pM (S/N = 3) for electrochemical detection, providing a reliable strategy for quantifying the Aβ protein in clinical applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c09760DOI Listing
July 2021
-->