Publications by authors named "Lina Wang"

930 Publications

Food reward depends on TLR4 activation in dopaminergic neurons.

Pharmacol Res 2021 May 7:105659. Epub 2021 May 7.

Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangzhou, Guangdong 510642, China; National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, China. Electronic address:

The rising prevalence of obesity and being overweight is a worldwide health concern. Food reward dysregulation is the basic factor for the development of obesity. Dopamine (DA) neurons in the ventral tegmental area (VTA) play a vital role in food reward. Toll-like receptor 4 (TLR4) is a transmembrane pattern recognition receptor that can be activated by saturated fatty acids. Here, we show that the deletion of TLR4 specifically in DA neurons increases body weight, increases food intake, and decreases food reward. Conditional deletion of TLR4 also decreased the activity of DA neurons while suppressing the expression of tyrosine hydroxylase (TH) in the VTA, which regulates the concentration of DA in the nucleus accumbens (NAc) to affect food reward. Meanwhile, AAV-Cre-GFP mediated VTA-specific TLR4-deficient mice recapitulates food reward of DAT-TLR4-KO mice. Food reward could be rescued by re-expressing TLR4 in VTA DA neurons. Moreover, effects of intra-VTA infusion of lauric acid (a saturated fatty acid with 12 carbon) on food reward were abolished in mice lacking TLR4 in DA neurons. Our study demonstrates the critical role of TLR4 signaling in regulating the activity of VTA DA neurons and the normal function of the mesolimbic DA system that may contribute to food reward.
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http://dx.doi.org/10.1016/j.phrs.2021.105659DOI Listing
May 2021

Increasing prevalence of cerebral palsy among children and adolescents in China 1988-2020: A systematic review and meta-analysis.

J Rehabil Med 2021 May 7. Epub 2021 May 7.

Department of Epidemiology and Biostatistics, School of Public Health, Southeast University, Nanjing, China.

To investigate the pooled prevalence of cerebral palsy in China, analyse the differences between different subgroups, and explore the trend over the 32-year period from 1988 to 2020. All potential studies related to the prevalence of cerebral palsy among children and adoles-cents in China were identified from 3 English- language databases and 4 Chinese-language databases. Pooled prevalence was calculated to estimate the prevalence of cerebral palsy among 0-18 years old and different geographical regions in China, using a random-effects meta-analysis model. Continuous fractional polynomial regression modelling was used to estimate the trend in prevalence of cerebral palsy over time. Subgroup analysis and meta-regression were conducted to investigate heterogeneity. Funnel plots and Egger's test were used to explore potential publication bias. The pooled prevalence of cerebral palsy over the study period among 0-18 years old and different geographical regions in China was 2.07% (95% confidence interval (95% CI) 1.66-2.47%), and the prevalence of cerebral palsy was higher in males compared with females (2.25% vs 1.59%), and in rural residents compared with urban residents (2.54% vs 1.9%), respectively. The prevalence of cerebral palsy varied significantly between different geographical regions. In subjects with birthweights < 2.5 and > 4 kg the prevalence of cerebral palsy was significantly higher than in subjects with birthweights between 2.5 and 4 kg. The trend in pooled prevalence of cerebral palsy increased continuously over the period studied, and could be divided into 3 stages; the mean annual increase in prevalence from 1988 to 1996 and from 2008 to 2019 was more rapid. Multivariate meta-regression found that the year of study was one of the sources of heterogeneity among overall prevalence. (p=0.006). The pooled prevalence of cerebral palsy over the 32-year period from 1988 to 2020 was 2.07%. There was an increasing trend in prevalence of cerebral palsy among children and adolescents in China over this period.
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http://dx.doi.org/10.2340/16501977-2841DOI Listing
May 2021

Perceived Discrimination and Symptoms of Cognitive Dysfuntion Among Middle-Age and Older Persons Living With HIV in China: A Multicenter Cross-Sectional Study of the Mediating Role of Mental Health Symptoms and Social Isolation.

J Assoc Nurses AIDS Care 2021 Apr 28. Epub 2021 Apr 28.

Shuyu Han, MSN, RN, is a Doctoral Candidate, School of Nursing, Fudan University, Shanghai, China. Yan Hu, PhD, RN, FAAN, is a Professor and Dean, School of Nursing, Fudan University, Shanghai, China, and is the Director of Fudan University Centre for Evidence-based Nursing: A Joanna Briggs Institute Centre of Excellence, Shanghai, China. Lina Wang, PhD, RN, is a Professor, School of Medicine, Huzhou University, Huzhou, Zhejiang, China. Yaolin Pei, PhD, is a Postdoctoral Fellow, Rory Meyers College of Nursing, New York University, New York, New York, USA. Zheng Zhu, PhD, RN, is a Faculty Member, School of Nursing, Fudan University, Shanghai, China, and a Core Researcher of Fudan University Centre for Evidence-based Nursing: A Joanna Briggs Institute Centre of Excellence, Shanghai, China. Xiang Qi, BSN, RN, is a Doctoral Candidate, Rory Meyers College of Nursing, New York University, New York, New York, USA. Bei Wu, PhD, is the Dean's Professor in Global Health, the Director for Global Health and Aging Research, and the Director for Research, Hartford Institute for Geriatric Nursing, Rory Meyers College of Nursing, New York University, New York, New York, USA.

Abstract: Perceived discrimination is associated with symptoms of cognitive dysfunction (SOCD) among middle-age and older persons living with HIV (PLWH). We aimed to explore how the association between perceived discrimination and SOCD was mediated by mental health symptoms and social isolation. The sample included 321 PLWH, ages 45 years and older, for a multicenter cross-sectional study. Structural equation modeling showed an acceptable model fit and a significant total indirect effect between perceived discrimination and SOCD. All three indirect effect pathways were significant, suggesting that perceived discrimination could influence SOCD through mental health symptoms, through social isolation, or through mental health symptoms and then social isolation. Our study demonstrates that perceived discrimination is a concern for the management of cognitive function among middle-age and older PLWH. Both mental health symptoms and social isolation are critical elements in the design and evaluation of interventions for promoting cognitive health.
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http://dx.doi.org/10.1097/JNC.0000000000000264DOI Listing
April 2021

Vibrational Spectral Analysis of Natisite (NaTiSiO) and its Structure Evolution in Water and Sulfuric Acid Solutions.

Materials (Basel) 2021 Apr 27;14(9). Epub 2021 Apr 27.

National Engineering Laboratory for Hydrometallurgical Cleaner Production Technology, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.

Natisite (NaTiSiO) is a layered sodium titanosilicate containing TiO square pyramids. The structure evolution of natisite in water and acid solutions is the basis for its potential applications. With NaSiO as the silicon source, natisite with the shape of the square sheet was selectively prepared from the hydrothermal method with 14.3 mol/L NaOH solution at 240 °C. Natisite has 20 Raman active modes and 22 infrared active modes from the first-principles calculations within density functional theory, and the calculated Raman and infrared spectra agree well with the experimental ones. The characteristic Raman peak at 844 cm is caused by the symmetric stretching of the apical Ti-O bond in the TiO unit, assigning to and modes. Natisite remains relatively stable in water with a sodium leaching percentage of lower than 6%. When washing with sulfuric acid solutions, the interlayer spacing of natisite is reduced due to the extensive removal of sodium ions, and an intermediate composed of SiO and newly formed TiO units may be formed. Moreover, after washing with water and acid solutions, 95.5%, 63.4%, and 35.2% of Na, Si, and Ti in natisite can be leached in total, respectively, resulting in the structural disintegration of natisite.
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http://dx.doi.org/10.3390/ma14092259DOI Listing
April 2021

The Development of a Personalized Symptom Management Mobile Health Application for Persons Living with HIV in China.

J Pers Med 2021 Apr 25;11(5). Epub 2021 Apr 25.

Rory Meyers College of Nursing, New York University, New York, NY 10010, USA.

Persons living with HIV (PLWH) continuously experience symptom burdens. Their symptom prevalence and severity are also quite different. Mobile health (mHealth) applications (apps) offer exceptional opportunities for using personalized interventions when and where PLWH are needed. This study aimed to demonstrate the development process of the symptom management (SM) app and the structure and content of it. Our research team systematically searched for evidence-based resources and summarized up-to-date evidence for symptom management and health education. Our multidisciplinary research team that included physicians, nurses, software engineers, and nursing professors, evaluated the structure and content of the drafted app. Both quantitative data and qualitative results were collected at a group discussion meeting. Quantitative data were scores of sufficient evidence, situational suitability, practicability, cost-effectiveness, and understandability (ranged from one to four) for 119 items of the app contents, including the health tracking module, the self-assessment module, coping strategies for 18 symptoms (80 items), medication management, complementary therapy, diet management, exercise, relaxation techniques, and the obtaining support module. The SM app was comprised of eight modules and provided several personalized symptom management functions, including assessing symptoms and receiving different symptom management strategies, tracking health indicators, and communicating with medical staff. The SM app was a promising and flexible tool for HIV symptom management. It provided PLWH with personalized symptom management strategies and facilitated the case management for medical staff. Future studies are needed to further test the app's usability among PLWH users and its effects on symptom management.
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http://dx.doi.org/10.3390/jpm11050346DOI Listing
April 2021

Inhibiting BDNF/TrkB.T1 receptor improves resiniferatoxin-induced postherpetic neuralgia through decreasing ASIC3 signaling in dorsal root ganglia.

J Neuroinflammation 2021 Apr 19;18(1):96. Epub 2021 Apr 19.

Department of Anesthesiology and Pain Management, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu, China.

Background: Postherpetic neuralgia (PHN) is a devastating complication after varicella-zoster virus infection. Brain-derived neurotrophic factor (BDNF) has been shown to participate in the pathogenesis of PHN. A truncated isoform of the tropomyosin receptor kinase B (TrkB) receptor TrkB.T1, as a high-affinity receptor of BDNF, is upregulated in multiple nervous system injuries, and such upregulation is associated with pain. Acid-sensitive ion channel 3 (ASIC3) is involved in chronic neuropathic pain, but its relation with BDNF/TrkB.T1 in the peripheral nervous system (PNS) during PHN is unclear. This study aimed to investigate whether BDNF/TrkB.T1 contributes to PHN through regulating ASIC3 signaling in dorsal root ganglia (DRGs).

Methods: Resiniferatoxin (RTX) was used to induce rat PHN models. Mechanical allodynia was assessed by measuring the paw withdrawal thresholds (PWTs). Thermal hyperalgesia was determined by detecting the paw withdrawal latencies (PWLs). We evaluated the effects of TrkB.T1-ASIC3 signaling inhibition on the behavior, neuronal excitability, and inflammatory response during RTX-induced PHN. ASIC3 short hairpin RNA (shRNA) transfection was used to investigate the effect of exogenous BDNF on inflammatory response in cultured PC-12 cells.

Results: RTX injection induced mechanical allodynia and upregulated the protein expression of BDNF, TrkB.T1, ASIC3, TRAF6, nNOS, and c-Fos, as well as increased neuronal excitability in DRGs. Inhibition of ASIC3 reversed the abovementioned effects of RTX, except for BDNF and TrkB.T1 protein expression. In addition, inhibition of TrkB.T1 blocked RTX-induced mechanical allodynia, activation of ASIC3 signaling, and hyperexcitability of neurons. RTX-induced BDNF upregulation was found in both neurons and satellite glia cells in DRGs. Furthermore, exogenous BDNF activated ASIC3 signaling, increased NO level, and enhanced IL-6, IL-1β, and TNF-α levels in PC-12 cells, which was blocked by shRNA-ASIC3 transfection.

Conclusion: These findings demonstrate that inhibiting BDNF/TrkB.T1 reduced inflammation, decreased neuronal hyperexcitability, and improved mechanical allodynia through regulating the ASIC3 signaling pathway in DRGs, which may provide a novel therapeutic target for patients with PHN.
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http://dx.doi.org/10.1186/s12974-021-02148-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054387PMC
April 2021

Germanium-regulated adsorption site preference on ruthenium electrocatalyst for efficient hydrogen evolution.

Chem Commun (Camb) 2021 Apr 22;57(32):3889-3892. Epub 2021 Mar 22.

State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.

A magnesiothermic reduction route has been presented to synthesize phase-pure germanides that are not readily available traditionally. The obtained ruthenium germanide (RuGe) serves as an efficient non-Pt electrocatalyst for hydrogen evolution, and its intrinsic activity is very close to that of Pt. Our combined theoretical and experimental study demonstrates that the remarkable performance is derived from the germanium-induced change in hydrogen site preference from hollow to efficient Ru top sites.
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http://dx.doi.org/10.1039/d1cc00559fDOI Listing
April 2021

Dynamic functional connectivity changes in Parkinson's disease patients with REM sleep behavior disorder.

Brain Res 2021 Apr 20;1764:147477. Epub 2021 Apr 20.

Department of Neurology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Electronic address:

Background: Rapid eye movement (REM) sleep behavior disorder (RBD) is one of the common nonmotor symptoms of Parkinson's disease (PD), characterized by frequently occurring REM sleep without muscle atonia. Our aim was to explore dynamic network connection changes in PD patients with RBD.

Method: On the basis of RBD screening questionnaire (RBDSQ), 126 PD patients were classified into those with probable RBD symptoms (PD-pRBD) and without probable RBD (PD-npRBD). We applied independent component analysis, sliding window approach and k-means clustering methods to clarify dynamic functional connectivity alterations.

Results: In contrast to PD-npRBD, PD-pRBD patients were liable to engage in a brain pattern mainly marked by weaker positive couplings between visual network (VIS) and default mode network (DMN), DMN and basal ganglia network (BG), and within DMN (State IV). In addition, we discovered that both PD patients with or without pRBD had shorter dwell time and fewer occurrences in State III, characterized by positive correlations between VIS and DMN, BG and DMN, and positive within-network coupling of sensorimotor network (SMN), relative to healthy controls.

Conclusions: Our study suggested that the weaker positive couplings between VIS and DMN, DMN and BG, and within DMN in State IV could be involved in the pathogenesis of PD patients with probable RBD on an overall level.
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http://dx.doi.org/10.1016/j.brainres.2021.147477DOI Listing
April 2021

A Potential Combination Therapy of Berberine Hydrochloride With Antibiotics Against Multidrug-Resistant .

Front Cell Infect Microbiol 2021 25;11:660431. Epub 2021 Mar 25.

School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering (Ministry of Education), Frontiers Science Center for Synthetic Biology, Tianjin University, Tianjin, China.

Multidrug-resistant (MDR) strains can cause severe infections in intensive care units, and are rapidly developing resistance to the last-resort of existing antibiotics, posing a major global threat to health care system. Berberine hydrochloride (BBH), a kind of isoquinoline alkaloids extracted from Berberis and other plants, has been widely used as an antibacterial medicine for its reliable therapeutic efficiency. The synergistic effects of BBH with antibiotics against MDR were determined. BBH alone had weak antimicrobial activity (e.g., MIC≥256 mg/L) against MDR . However, it dramatically increased the susceptibility of MDR strains against antibiotics with FICI values <0.5, even reversed their resistance to antibiotics (e.g., tigecycline, sulbactam, meropenem and ciprofloxacin). study has suggested BBH with sulbactam had stronger antimicrobial efficiency than monotherapy in a neutropenic murine thigh infection model. The antibiotic-sensitizing mechanism of action of BBH was evaluated as well. BBH boosted gene expression and bound to the AdeB transporter protein, resulting in low uptake of BBH, which may contribute to less extrusion of antibiotics by the AdeABC pump. Knockout of the gene increased uptake of BBH and diminished the antibiotic sensitization and synergistic effects between antibiotics and BBH in MDR strains. Together, BBH effectively re-sensitizes this MDR pathogen to a range of antibiotics that have become barely effective due to antibiotic resistance, which indicates BBH may be a promising therapeutic adjuvant candidate to combat MDR .
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http://dx.doi.org/10.3389/fcimb.2021.660431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027359PMC
March 2021

Protective mechanism of Astragalus Polysaccharides against Cantharidin-induced liver injury determined in vivo by liquid chromatography/mass spectrometry metabolomics.

Basic Clin Pharmacol Toxicol 2021 Apr 9. Epub 2021 Apr 9.

Guizhou University of Traditional Chinese Medicine, Guiyang, China.

Cantharidin (CTD) is a promising anticancer drug; however, its dosage is limited by hepatotoxicity. We previously showed that Astragalus polysaccharides (APS) effectively improved chemical liver injury. In this study, we established a CTD-induced subacute liver injury mouse model and examined the effects of APS on weight, liver indexes, histopathology, serum biochemical indexes and liver metabolism. Compared with the control group, mice in the CTD model group had obvious liver damage, which was partially prevented by APS. Metabolomics demonstrated that CTD caused liver damage mainly by regulating glycerophospholipid metabolism, ABC transporter pathways and choline metabolism in cancer in vivo. APS regulated primary bile acid biosynthesis and glycerophospholipid metabolism, thus decreasing the liver damage caused by CTD. This study revealed the protective mechanism of APS against CTD-induced liver injury from the perspective of metabolomics. The results provide an important basis for analysing the mechanism of CTD-induced liver toxicity and for assessing clinical treatment options to reduce CTD liver toxicity.
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http://dx.doi.org/10.1111/bcpt.13585DOI Listing
April 2021

Variations of microbial community in Aconitum carmichaeli Debx. rhizosphere soilin a short-term continuous cropping system.

J Microbiol 2021 May 29;59(5):481-490. Epub 2021 Mar 29.

National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China.

Aconitum carmichaeli Debx. (Ranunculaceae) is a potential source of an important herbal drug named "Fuzi", which is derived from the lateral root of the plant. Increased therapeutic usage resulted in the great demand for artificial cultivation of A. carmichaeli, however, the obstacles caused by continuous cropping is a serious problem. Continuous cropping has shown to affect the soil biological and non-biological factors. The current study attempted to discover the variations of microbial communities and soil properties in short-term continuous cropping of A. carmichaeli. An experimental procedure with A. carmichaeli planted two years continuously was established. The variation of the soil microbial community, disease incidence, soil properties, and the correlation between soil microbe and disease incidence were investigated. The disease incidence increased during the continuous cropping of A. carmichaeli. The PCoA and LefSe results indicated that fungal communities in rhizosphere soil were altered during the short-term continuous croppingand the bacterial community was disturbed by the cultivation of A. carmichaeli, however, in the following two years of continuous cropping period, the soil bacterial community has not changed obviously. Proportions of some fungal and bacterial genera were varied significantly (p < 0.05), and some genera of microflora showed a significant correlation with adisease incidence of A. carmichaeli. Microorganisms contributing to community composition discrepancy were also elucidated. Continuous cropping of A. carmichaeli disturbed the rhizosphere soil microbial community and altered the soil chemical parameters and soil pH. These variations in soil may be related to the occurrence of plant diseases. The current study will not only provide theoretical and experimental evidence for the A. carmichaeli continuous cropping obstacles but will also contribute to A. carmichaeli agricultural production and soil improvement.
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http://dx.doi.org/10.1007/s12275-021-0515-zDOI Listing
May 2021

Association of Maternal Body Mass Index With Risk of Infant Mortality: A Dose-Response Meta-Analysis.

Front Pediatr 2021 12;9:650413. Epub 2021 Mar 12.

Department of Obstetrics, Maternity and Child Health Care of Zaozhuang, Zaozhuang, China.

This study presumed that a high or low body mass index (BMI) might increase the risk of infant mortality. Therefore, a meta-analysis was performed to systematically assess the association between maternal BMI and the risk of infant mortality. The electronic databases, including Pubmed, Embase database, and Cochrane Library, were systemically searched by two investigators from inception to November 26th, 2020, with no language restriction. In parallel, a dose-response was assessed. Finally, 22 cohort studies involving 13,532,293 participants were included into this paper, which showed that compared with normal BMI, maternal overweight significantly increased the risks of infant mortality [risk ratio (RR), 1.16; 95% confidence interval (CI), 1.13-1.19], neonatal mortality (RR, 1.23; 95% CI, 1.08-1.39), early neonatal mortality (RR, 1.55; 95% CI, 1.26-1.92) and post-neonatal mortality (RR, 1.18; 95% CI, 1.07-1.29). Similarly, maternal obesity significantly increased the risk of infant mortality (RR, 1.55; 95% CI, 1.41-1.70), neonatal mortality (RR, 1.55; 95% CI, 1.28-1.67), early neonatal mortality (RR, 1.37; 95% CI, 1.13-1.67), and post-neonatal mortality (RR, 1.30; 95% CI, 1.03-1.65), whereas maternal underweight potentially decreased the risk of infant mortality (RR, 0.93; 95% CI, 0.88-0.98). In the dose-response analysis, the risk of infant mortality significantly increased when the maternal BMI was >25 kg/m. Maternal overweight or obesity significantly increases the risks of infant mortality, neonatal mortality, early neonatal mortality, and post-neonatal mortality compared with normal BMI in a dose-dependent manner. Besides, maternal underweight will not increase the risk of infant mortality, neonatal mortality, early neonatal mortality, or postneonatal mortality; instead, it tends to decrease the risk of infant mortality. Early weight management may provide potential benefits to infants, and more large-scale prospective studies are needed to verify this finding in the future.
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http://dx.doi.org/10.3389/fped.2021.650413DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994890PMC
March 2021

PHD3 mediates denervation skeletal muscle atrophy through Nf-κB signal pathway.

FASEB J 2021 Apr;35(4):e21444

Guangdong Laboratory of Lingnan Modern Agriculture, National Engineering Research Center for Breeding Swine Industry and Guangdong Province Key Laboratory of Animal Nutritional Regulation, College of Animal Science, South China Agricultural University, Guangzhou, China.

Skeletal muscle is the largest organ of the body, the development of skeletal muscle is very important for the health of the animal body. Prolyl hydroxylases (PHDs) are the classical regulator of the hypoxia inducible factor (HIF) signal pathway, many researchers found that PHDs are involved in the muscle fiber type transformation, muscle regeneration, and myocyte differentiation. However, whether PHDs can impact the protein turnover of skeletal muscle is poorly understood. In this study, we constructed denervated muscle atrophy mouse model and found PHD3 was highly expressed in the atrophic muscles and there was a significant correlation between the expression level of PHD3 and skeletal muscle weight which was distinct from PHD1 and PHD2. Then, the similar results were getting from the different weight muscles of normal mice. To further verify the relationship between PHD3 and skeletal muscle protein turnover, we established a PHD3 interference model by injecting PHD3 sgRNA virus into tibialis anterior muscle (TA) muscle of MCK-Cre-cas9 mice and transfecting PHD3 shRNA lentivirus into primary satellite cells. It was found that the Knock-out of PHD3 in vivo led to a significant increase in muscle weight and muscle fiber area (P < .05). Besides, the activity of protein synthesis signal pathway increased significantly, while the protein degradation pathway was inhibited evidently (P < .05). In vitro, the results of 5-ethynyl-2'-deoxyuridine (EdU) and tetramethylrhodamine ethyl ester (TMRE) fluorescence detection showed that PHD3 interference could lead to a decrease in cell proliferation and an increase of cell apoptosis. After the differentiation of satellite cells, the production of puromycin in the interference group was higher than that in the control group, and the content of 3-methylhistidine in the interference group was lower than that in the control group (P < .05) which is consistent with the change of protein turnover signal pathway in the cell. Mechanistically, there is an interaction between PHD3, NF-κB, and IKBα which was detected by immunoprecipitation. With the interfering of PHD3, the expression of the inflammatory signal pathway also significantly decreased (P < .05). These results suggest that PHD3 may affect protein turnover in muscle tissue by mediating inflammatory signal pathway. Finally, we knocked out PHD3 in denervated muscle atrophy mice and LPS-induced myotubes atrophy model. Then, we found that the decrease of PHD3 protein level could alleviate the muscle weight and muscle fiber reduction induced by denervation in mice. Meanwhile, the protein level of the inflammatory signal pathway and the content of 3-methylhistidine in denervated atrophic muscle were also significantly reduced (P < .05). In vitro, PHD3 knock-out could alleviate the decrease of myotube diameter induced by LPS, and the expression of protein synthesis pathway was also significantly increased (P < .05). On the contrary, the expression level of protein degradation and inflammatory signal pathway was significantly decreased (P < .05). Through these series of studies, we found that the increased expression of PHD3 in denervated muscle might be an important regulator in inducing muscle atrophy, and this process is likely to be mediated by the inflammatory NF-κB signal pathway.
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http://dx.doi.org/10.1096/fj.202002049RDOI Listing
April 2021

Hypoxanthine Induces Muscular ATP Depletion and Fatigue via UCP2.

Front Physiol 2021 3;12:647743. Epub 2021 Mar 3.

Guangdong Laboratory of Lingnan Modern Agriculture, National Engineering Research Center for Breeding Swine Industry and Guangdong Province Key Laboratory of Animal Nutritional Regulation, College of Animal Science, South China Agricultural University, Guangzhou, China.

Hypoxanthine (Hx), an intermediate metabolite of the purine metabolism pathway which is dramatically increased in blood and skeletal muscle during muscle contraction and metabolism, is characterized as a marker of exercise exhaustion. However, the physiological effects of Hx on skeletal muscle remain unknown. Herein, we demonstrate that chronic treatment with Hx through dietary supplementation resulted in skeletal muscle fatigue and impaired the exercise performance of mice without affecting their growth and skeletal muscle development. Hx increased the uncoupling protein 2 (UCP2) expression in the skeletal muscle, which led to decreased energy substrate storage and enhanced glycolysis. These effects could also be verified in acute treatment with Hx through intraperitoneal injection. In addition, muscular specifically knockout of UCP2 through intra-muscle tissue injection of adenovirus-associated virus reversed the effects of Hx. In conclusion, we identified a novel role of Hx in the skeletal muscular fatigue mediated by UCP2-dependent mitochondrial uncoupling. This finding may shed light on the pathological mechanism of clinical muscle dysfunctions due to abnormal metabolism, such as muscle fatigue and weakness.
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http://dx.doi.org/10.3389/fphys.2021.647743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966526PMC
March 2021

Exploration of the cortical pathophysiology underlying visual disturbances in schizophrenia comorbid with depressive disorder-An evidence from mouse model.

Brain Behav 2021 Mar 17:e02113. Epub 2021 Mar 17.

Laboratory of Psychiatric-Neuroimaging-Genetic and Cor-morbidity (PNGC_Lab), Tianjin Anding Hospital, Mental Health Centre of Tianjin, Affiliated Teaching Hospital of Tianjin Medical University, Tianjin, China.

Introduction: Patients with schizophrenia frequently present with visual disturbances including hallucination, and this symptom is particularly prevalent in individuals with comorbid depressive disorders. Currently, little is known about the neurobiological mechanisms of such psychiatric symptoms, and few explanations for the co-occurrence of schizophrenia, depression, and visual disturbances are available.

Methods: In this study, we generated a mouse schizophrenia model in which depressive symptoms were also induced. We adopted in vivo two-photon calcium imaging and ex vivo electrophysiological recording of the primary visual cortex to reveal the synaptic transmission and neural activity in the mouse schizophrenia model.

Results: In vivo two-photon calcium imaging and ex vivo electrophysiological recording of the primary visual cortex revealed impaired synaptic transmission and abnormal neural activity in the schizophrenia model, but not in the depression model. These functional deficits were most prominent in the combined schizophrenia and depression model.

Conclusion: Overall, our data support a mechanism by which the visual cortex plays a role in visual disturbances in schizophrenia.
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http://dx.doi.org/10.1002/brb3.2113DOI Listing
March 2021

FBS-Derived Exosomes as a Natural Nano-Scale Carrier for Icariin Promote Osteoblast Proliferation.

Front Bioeng Biotechnol 2021 26;9:615920. Epub 2021 Feb 26.

School of Stomatology, Dalian Medical University, Dalian, China.

Icariin is a class IV drug of low solubility, permeability, and poor bioavailability. Synthetic nanomaterials have developed rapidly. However, some literatures point out that synthetic nanomaterials such as liposomes, aptamers, metal nanoparticles, and nanogels have high toxicity and are affected by the reticuloendothelial system or mononuclear phagocyte system. It is known that exosomes could be used as an ideal clinical drug delivery vehicle to avoid the above-mentioned problems to a certain extent. Studies have shown that drugs can be loaded into exosomes by passive and active loading. We used Fetal bovine serum (FBS) exosomes to carry Icariin for the first time in this experiment, FBS exosomes-Icariin (FBS EXO-ICA) more effectively promoted the proliferation of osteoblasts and bone regeneration than Icariin alone. FBS EXO-ICA could become a new nano scale drug formulation for treating diseases associated with bone loss.
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http://dx.doi.org/10.3389/fbioe.2021.615920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952636PMC
February 2021

Peritoneal resident macrophages in mice with MLL-AF9-induced acute myeloid leukemia show an M2-like phenotype.

Ann Transl Med 2021 Feb;9(3):266

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.

Background: Acute myeloid leukemia (AML) is a devastating disease with a poor prognosis. Innate and adaptive immunity is closely related to the progression of leukemia. Macrophages within the leukemic microenvironment have a tendency toward a leukemia-permissive phenotype. However, the characteristics of macrophages in leukemia, including their kinetics, gene expression, and functional roles have not been fully illuminated.

Methods: In the current study, the characteristics of peritoneal resident macrophages, which were large peritoneal macrophages (LPM), from mice with mixed lineage leukemia (MLL)-AF9-induced AML were investigated. AML-associated large macrophages (AML-LPM) were gated as F4/80 MHC-II by flow cytometry. To further investigate the relationship between the leukemic microenvironment and macrophage characteristics, RNA sequencing was performed. Meanwhile, apoptosis, killing ability, and phagocytic function of peritoneal resident macrophages in MLL-AF9-induced AML were assessed.

Results: The results suggested that AML microenvironment was found to affect the kinetics and morphology of peritoneal resident macrophages. The results of RNA sequencing suggested that the gene expression of AML-LPMs differed significantly from that of normal LPMs. The AML microenvironment also had effects on the apoptosis, killing ability, and phagocytic function of peritoneal resident macrophages.

Conclusions: These data suggest that peritoneal resident macrophages in mice with AML induced by MLL-AF9 show an M2-like phenotype. The reversal of macrophage polarization in the leukemic microenvironment may potentially enhance the immunotherapeutic effect in AML.
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http://dx.doi.org/10.21037/atm-21-139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940882PMC
February 2021

Predictive risk factors for sentinel lymph node metastasis using preoperative contrast-enhanced ultrasound in early-stage breast cancer patients.

Gland Surg 2021 Feb;10(2):761-769

Department of Breast Surgery, Affiliated Cancer Hospital of Zhengzhou University (Henan Cancer Hospital), Zhengzhou, China.

Background: Sentinel lymph node biopsy (SLNB) is the standard procedure for axillary staging in clinically node-negative (cN0) breast cancer patients. The positive rate of SLNs in cN0 stage patients ranges from 20.5% to 25.5%, so identifying appropriate candidates for SLNB is quite challenging. The aims of this study were to assess whether contrast-enhanced ultrasound (CEUS) could be utilized to noninvasively predict SLN metastasis, and to explore the predictive value of the involved factors.

Methods: Between May 2016 and May 2018, 217 consenting breast cancer patients undergoing SLNB were enrolled. Before the surgery, CEUS was utilized to identify the SLNs, and predict whether metastasis had occurred according to their enhancement pattern. Blue dye was also used to identify the SLNs during SLNB. The rates of identification and accuracy of both methods were recorded. The predictive outcomes of SLNs identified by CEUS were recorded and compared with the pathological diagnosis.

Results: Of the 217 cases, SLNs in 212 cases were successfully identified, comprising 208 cases identified by CEUS and 206 cases by blue dye, with no significant difference between the two methods (P=0.6470). A total of 78 cases were predicted SLN-positive preoperatively by CEUS, comprising 61 cases of SLN metastasis confirmed by pathology and 17 cases of no SLN metastasis, and 130 cases were predicted SLN-negative by CEUS, comprising 6 cases of SLN metastasis and 124 cases of no SLN metastasis. The sensitivity of CEUS preoperative prediction was 91.0%, the specificity was 87.9%, the positive and negative predictive values were 78.2% and 95.4%, respectively, and the accuracy was 88.9%. The maximum diameter size of positive SLNs predicted by CEUS was greater than that of negative SLNs (mean value 1.67±0.06 . 1.40±0.05 cm, P=0.0007). Similarly, the primary tumor size predicted SLN-positive by CEUS was greater than that in patients with negative SLNs (mean value 2.64±0.12 . 1.79±0.09 cm, P<0.0001).

Conclusions: CEUS accurately identified SLNs and can be used to noninvasively predict SLN metastasis in early-stage breast cancer patients. However, the primary tumor size and the SLN size should not be overlooked by clinicians when judging the status of SLNs. This novel method may be a recommended strategy for identifying appropriate SLNB candidates.
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http://dx.doi.org/10.21037/gs-20-867DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944082PMC
February 2021

Smell disorders in COVID-19 patients: role of olfactory training: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Feb;100(8):e24862

School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine.

Background: As the coronavirus disease 2019 (COVID-19) spread around the world, a surge of evidence suggests that smell disorders are common symptoms in COVID-19 infection. This dysfunction may cause loss of appetite, malnutrition, poisoning, and depression. Obviously, the impairment has a strong impact on the quality of life. Therefore, there is an urgent need to identify effective treatments. Various therapies have been studied to treat smell disorders after infection, and olfactory training (OT) is considered a promising treatment option. Assessing the effectiveness and safety of olfactory training for COVID-19 patients with smell disorders is the main purpose of this systematic review protocol.

Methods: PubMed, EMBASE, MEDLINE, the Cochrane Library, Chinese National Knowledge Infrastructure, Chinese Biomedical Literature Database, Wanfang Database, ClinicalTrials.gov trials registry, and Chinese Clinical Trial Registry will be searched from January 2019 to January 2021. A combination of subject words and free text words will be applied in the searches. The language is limited to Chinese and English. The complete process will include study selection, data extraction, risk of bias assessment, and meta-analyses. Endnote X9.3 will be used to manage data screening. The statistical analysis will be completed by Review Manager V.5.3 (Cochrane Collaboration) or Stata V.16.0 software.

Results: This proposed study will assess the effectiveness and safety of OT for COVID-19 patients with smell disorders.

Conclusion: The conclusion of this study will provide evidence to prove the effectiveness and safety of olfactory training for COVID-19 patients with smell disorders.

Ethics And Dissemination: This protocol will not evaluate individual patient information or infringe patient rights and therefore does not require ethical approval.

Registration: PEROSPERO CRD42020218009.
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http://dx.doi.org/10.1097/MD.0000000000024862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909207PMC
February 2021

The simplest alkynyl thiocyanate HCCSCN and its isomers.

Chem Commun (Camb) 2021 Apr 4;57(27):3343-3346. Epub 2021 Mar 4.

Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysts and Innovative Materials, Fudan University, Shanghai 200433, China.

The simplest alkynyl thiocyanate HCCSCN has been synthesized and characterized for the first time. HCCSCN is surprisingly stable at room temperature but undergoes photoisomerization in an Ar-matrix (10 K) to yield the novel sulfenyl isocyanide HCCSNC and thioketene NCC(H)CS. The isomeric isothiocyanate HCCNCS, missing in the photochemistry of HCCSCN, can be generated through the photodecarbonylation of propiolyl isothiocyanate.
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http://dx.doi.org/10.1039/d1cc00629kDOI Listing
April 2021

Relationship between emotional intelligence and job well-being in Chinese Registered Nurses: Mediating effect of communication satisfaction.

Nurs Open 2021 Mar 3. Epub 2021 Mar 3.

College of Nursing, Xinxiang Medical University, Xinxiang City, China.

Aim: To explore the relationship between emotional intelligence and job well-being among Chinese Registered Nurses, and the mediating role of communication satisfaction in the relationship.

Design: A descriptive cross-sectional study design was employed.

Methods: Random sampling was adopted. The study was conducted from September to December 2019, and 1,475 Registered Nurses from a Chinese hospital provided responses to Wong and Law's emotional intelligence scale, communication satisfaction scale, job well-being scale and general information questionnaire. SPSS25.0 software was used to calculate means, standard deviations and correlations, and AMOS 21.0 software was used to establish the structural equation model.

Results: The emotional intelligence, communication satisfaction and job well-being of Registered Nurses in China were related to positional rank, work department, monthly income, years of service, night shift work distribution and intensity of work. There were positive correlations among emotional intelligence, communication satisfaction and job well-being. Communication satisfaction partly mediated the relationship between emotional intelligence and job well-being. Improving the level of emotional intelligence and communication satisfaction should be an important strategy to improve nurses' job well-being. Therefore, nursing managers could carry out targeted training on emotional intelligence management and communication between nurses and patients and pay attention to the spiritual needs of nurses and provide psychological guidance on a regular basis.
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http://dx.doi.org/10.1002/nop2.820DOI Listing
March 2021

MiR-23a Is Involved in Myocardial Ischemia/Reperfusion Injury by Directly Targeting CX43 and Regulating Mitophagy.

Inflammation 2021 Mar 2. Epub 2021 Mar 2.

Department of Cardiovascular Medicine, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710004, China.

Activation of CX43 signaling protects myocardial cells from myocardial ischemia/reperfusion (I/R) injury. However, the underlying mechanism remains unclear. MicroRNAs (miRNAs) are well known to play important roles in the progression of diverse diseases. Here, we first confirmed the expression profile of CX43 in rat heart tissues with I/R injury. Then, microRNAs (miRNAs) that target CX43 were predicted using miRDB, miRWalk, and TargetScan. The candidate miR-23a was selected, and its expression level in I/R samples was investigated. To determine the role of miR-23a, rat primary myocardial cells were transfected with miR-23a mimics after they were subjected to hypoxia-reoxygenation (H/R) injury. Transfection of miR-23a mimics stimulated mitophagy through the PINK1/Parkin pathway and downregulated the protein level of CX43. Treatment of miR-23a-transfected cells with NF-kB inhibitors completely abolished miR-23a-mediated mitophagy after H/R. Moreover, miR-23a transfection significantly suppressed CX43 expression and enhanced mitophagy in the model heart in vivo. Therefore, miR-23a plays a detrimental role in myocardial I/R injury by enhancing mitophagy and inhibiting CX43 mRNA.
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http://dx.doi.org/10.1007/s10753-021-01443-wDOI Listing
March 2021

Identification of mast cells as a candidate significant target of immunotherapy for acute myeloid leukemia.

Hematology 2021 Dec;26(1):284-294

Department of Hematology, the First Hospital of Lanzhou University, Lanzhou, People's Republic of China.

Objection: Immunotherapy based on T cells is a new therapy for Acute myeloid leukemia (AML). However, there has not been considerable improvement compared with traditional chemotherapeutics. This study aimed to identify important immune cells, genes, and drugs associated with the immunotherapy of AML.

Methods: The gene expression profile and clinical data of patients with AML were downloaded from TCGA database, and the abundance ratio of immune cells was obtained via CIBERSORT. Kaplan-Meier (KM) survival analysis was used to assess the relationship between immune cells and survival time of patients with AML. Differentially expressed genes (DEGs) analysis was conducted to obtained DEGs related to mast cells. Then, protein-protein interaction (PPI) analysis and enrichment analysis were performed to explore the hub genes. Finally, Connectivity Map (CMap) database was utilized to predicts potential drugs that may reverse or induce the mast cell-related gene expression.

Results: Our study showed that mast cell was correlated with survival time of patients with AML, and 135 genes were screened to be related with mast cells. 6 hub genes were identified via PPI network, and 3 potential small molecule drugs were screened to be related to regulating the mast cell-related gene expression via CMap database.

Conclusion: The hub genes and drugs have high research value and clinical application in AML therapy. Our study not only provides gene targets and small molecule drugs for AML immunotherapy concerning mast cells but also provides new ideas for researchers to explore immunotherapy targets of other tumors.
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http://dx.doi.org/10.1080/16078454.2021.1889158DOI Listing
December 2021

Neuron specific enolase promotes tumor metastasis by activating the Wnt/β-catenin pathway in small cell lung cancer.

Transl Oncol 2021 Apr 19;14(4):101039. Epub 2021 Feb 19.

Department of Medical Oncology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, 510180, Guangdong, China; Department of Medical Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, Guangdong, China. Electronic address:

Neuron-specific enolase (NSE) has been used as a specific biomarker for small cell lung cancer (SCLC) patients. Nevertheless, the biological function and mechanism of NSE in SCLC are still unclear. In this study, we clarified the role of NSE in the progression of SCLC and found that NSE expression was positively correlated with distant metastasis. Functional analysis showed that overexpression of NSE promoted migration and invasion of SCLC cells. Mechanism analysis showed that NSE overexpression induced epithelial-mesenchymal transition (EMT) of SCLC cells. Moreover, overexpression of NSE increased the protein expression of β-catenin and its downstream target genes, and silencing β-catenin eliminated NSE-mediated cell migration, invasion and EMT process. Furthermore, NSE interacted with β-catenin and inhibited the degradation of β-catenin. Besides, the animal experiments also indicated that NSE could promote the EMT process and distant metastasis of SCLC cells in vivo. In summary, our results revealed that NSE could promote the EMT process of SCLC cells by activating the Wnt/β-catenin signaling pathway, thereby promoting cell migration, invasion and distant metastasis, which might serve as a potential target for the therapy of SCLC patients.
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http://dx.doi.org/10.1016/j.tranon.2021.101039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905480PMC
April 2021

Environmental Indicator for COVID-19 Non-Pharmaceutical Interventions.

Geophys Res Lett 2021 Jan 21;48(2):e2020GL090344. Epub 2021 Jan 21.

Gansu Province Environmental Monitoring Center Lanzhou China.

A novel coronavirus (COVID-19) has caused viral pneumonia worldwide, posing a major threat to international health. Our study reports that city lockdown is an effective way to reduce the number of new cases and the nitrogen dioxide (NO) concentration can be used as an environmental lockdown indicator to evaluate the effectiveness of lockdown measures. The airborne NO concentration steeply decreased over the vast majority of COVID-19-hit areas since the lockdown. The total number of newly confirmed cases reached an inflection point about two weeks since the lockdown and could be reduced by about 50% within 30 days of the lockdown. The stricter lockdown will help newly confirmed cases to decline earlier and more rapidly, and at the same time, the reduction rate of NO concentration will increase. Our research results show that NO satellite observations can help decision makers effectively monitor and manage non-pharmaceutical interventions in the epidemic.
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http://dx.doi.org/10.1029/2020GL090344DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883230PMC
January 2021

Synthesis and characterization of phosphorous(III) diisocyanate and triisocyanate.

Dalton Trans 2021 Mar;50(9):3299-3307

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, China and Department of Chemistry, Fudan University, Shanghai, 200433, China.

Two phosphorous(iii) isocyanates, ClP(NCO)2 and P(NCO)3 were isolated as neat substances and characterized with IR (gas-phase and Ne-matrix), Raman (solid), and 31P NMR spectroscopy. Their vibrational spectra were analyzed in terms of a single conformer with the aid of quantum chemical computations at the B3LYP/6-311+G(3df) level of theory. In line with the theoretically computed favorable syn-configuration of the NCO ligands with the sterically active lone-pair electrons on the central phosphorous atom (nP), low-temperature single-crystal X-ray diffraction (XRD) of solid ClP(NCO)2 reveals a Cs symmetric syn-configuration for both NCO ligands with weak CO (r = 2.9692(4) Å) van der Waals (vdW) interactions. In the binary isocyante P(NCO)3, all the three NCO ligands adopt similar syn-configuration with nP, leading to a propeller-shaped structure with slightly distorted C3v symmetry due to steric repulsion of the NCO ligands and the PO vdW interactions (r = 3.1901(1) Å) in the solid state.
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http://dx.doi.org/10.1039/d1dt00261aDOI Listing
March 2021

Prevalence of mental disorders in 21st century Shandong Province, China: A ten-year comparative study.

J Affect Disord 2021 03 2;283:344-353. Epub 2021 Feb 2.

Department of Biostatistics, School of Public Health, Cheeloo College of Medicine, Shandong University, 44 Wenhuaxi Road, Lixia district, Jinan, 250012 China; Institute for Medical Dataology, Cheeloo College of Medicine, Shandong University, Jinan, China. Electronic address:

Background: Although the 2000s observed enormous changes in China, little is known about the variation in the prevalence of mental disorders. The study compared the prevalence in a Chinese population between 2004 and 2015.

Methods: Multistage stratified random sampling methods were used to identify primary sampling sites for cross-sectional surveys in 2004 and 2015 in Shandong, China. In 2004 and 2015, 22,718 and 28,194 adults, respectively, completed an expanded version of the General Health Questionnaire, then 5,402 and 9,420 adults, respectively, were administered a Chinese version of the Structured Clinical Interview for Diagnostic and Statistical Manual-IV axis I disorders.

Results: The adjusted 1-month prevalence of any mental disorders was 18•7% (95% CI: 16•7-20•8) and 17•1% (95% CI:15•9-18•4) in 2004 and 2015, respectively. However, the prevalence of major depressive disorders increased from 1•5% (95% CI: 1•2 -1•8) in 2004 to 2•3% (95% CI: 1•9-2•8) in 2015; meanwhile the prevalence of alcohol abuse disorders were becoming more common among men and urban residents. Although mood and anxiety disorders were more prevalent in women, a much more prevalent alcohol abuse disorders for men contributed to a higher overall prevalence among men than among women. Compared to that in urban residents, the overall prevalence in rural residents declined more, and it was lower in 2015 than in 2004.

Limitations: The results may not apply to the population from other regions.

Conclusions: Despite of the stable overall prevalence, mental disorders beyond psychotic disorders should be focused on, especially alcohol abuse and major depressive disorders.
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http://dx.doi.org/10.1016/j.jad.2021.01.068DOI Listing
March 2021

Multiperspective Progressive Structure Adaptation for JPEG Steganography Detection Across Domains.

IEEE Trans Neural Netw Learn Syst 2021 Feb 9;PP. Epub 2021 Feb 9.

The aim of steganography detection is to identify whether the multimedia data contain hidden information. Although many detection algorithms have been presented to solve tasks with inconsistent distributions between the source and target domains, effectively exploiting transferable correlation information across domains remains challenging. As a solution, we present a novel multiperspective progressive structure adaptation (MPSA) scheme based on active progressive learning (APL) for JPEG steganography detection across domains. First, the source and target data originating from unprocessed steganalysis features are clustered together to explore the structures in different domains, where the intradomain and interdomain structures can be captured to provide adequate information for cross-domain steganography detection. Second, the structure vectors containing the global and local modalities are exploited to reduce nonlinear distribution discrepancy based on APL in the latent representation space. In this way, the signal-to-noise ratio (SNR) of a weak stego signal can be improved by selecting suitable objects and adjusting the learning sequence. Third, the structure adaptation across multiple domains is achieved by the constraints for iterative optimization to promote the discrimination and transferability of structure knowledge. In addition, a unified framework for single-source domain adaptation (SSDA) and multiple-source domain adaptation (MSDA) in mismatched steganalysis can enhance the model's capability to avoid a potential negative transfer. Extensive experiments on various benchmark cross-domain steganography detection tasks show the superiority of the proposed approach over the state-of-the-art methods.
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http://dx.doi.org/10.1109/TNNLS.2021.3054045DOI Listing
February 2021

Comprehensive evaluation of candidate reference genes for quantitative real-time PCR-based analysis in Caucasian clover.

Sci Rep 2021 Feb 8;11(1):3269. Epub 2021 Feb 8.

College of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Street, Xiangfang District, Harbin City, Heilongjiang, China.

The forage species Caucasian clover (Trifolium ambiguum M. Bieb.), a groundcover plant, is resistant to both cold and drought. However, reference genes for qRT-PCR-based analysis of Caucasian clover are lacking. In this study, 12 reference genes were selected on the basis of transcriptomic data. These genes were used to determine the most stably expressed genes in various organs of Caucasian clover under cold, salt and drought stress for qRT-PCR-based analysis. Reference gene stability was analyzed by geNorm, NormFinder, BestKeeper, the ∆Ct method and RefFinder. Under salt stress, RCD1 and PPIL3 were the most stable reference genes in the leaves, and NLI1 and RCD1 were the most stable references genes in the roots. Under low-temperature stress, APA and EFTu-GTP were the most stable reference genes in the leaves, and the RCD1 and NLI2 genes were highly stable in the roots. Under 10% PEG-6000 stress, NLI1 and NLI2 were highly stable in the leaves, and RCD1 and PPIL3 were the most stable in the roots. Overall, RCD1 and NLI2 were the most stable reference genes in organs under normal conditions and across all samples. The most and least stable reference genes were validated by assessing their appropriateness for normalization via WRKY genes.
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http://dx.doi.org/10.1038/s41598-021-82633-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870939PMC
February 2021

Sleep quality as a mediator of the association between coping styles and mental health: a population-based ten-year comparative study in a Chinese population.

J Affect Disord 2021 03 27;283:147-155. Epub 2021 Jan 27.

Department of Biostatistics, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, China; Institute for Medical Dataology, Cheeloo College of Medicine, Shandong University, Jinan, China.

Backgrounds: Little is known about the variation in sleep quality and its association with coping style and mental health in 21 century China, despite of enormous socioeconomic changes. This study aims to document the variation in sleep quality and its contribution to the association between coping style and mental health in China.

Methods: Pooled cross-sectional data of 46,561 adults was obtained from the 2004 and 2015 mental health surveys conducted in Shandong Province, China. A Simplified Coping Style Questionnaire and the Pittsburgh Sleep Quality Index were assessed, with mental health measured by the General Health Questionnaire (GHQ). A mediation regression model was run to test the mediating effect of sleep quality.

Results: Above 10% reported poor sleep quality or median-to-high risk of mental disorders according to GHQ results in year 2015, and a significant but small improvement for sleep quality and mental health came during the studied decade, with the exception of poor sleep quality increasing among males. In 2015, a one-point increase in sleep quality score was associated with an increase of 0.17 (95% CI, 0.16-0.18) and 0.16 (95% CI, 0.14-0.17) points on the GHQ for males and females, respectively. Sleep quality mediated the relationship between negative tendency of coping style and elevated GHQ scores, and the mediating effects grew stronger in 2015 than those in 2004.

Limitation: The study is a cross-sectional study, and the sample is not nationally representative.

Conclusion: An integrative intervention of mental health promotion is recommended to account for sleep quality and coping strategies..
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http://dx.doi.org/10.1016/j.jad.2021.01.045DOI Listing
March 2021