Publications by authors named "Lina Li"

251 Publications

Detection of BCOR gene rearrangement in Ewing-like sarcoma: an important diagnostic tool.

Diagn Pathol 2021 Jun 8;16(1):50. Epub 2021 Jun 8.

Department of Pathology, Beijing Jishuitan Hospital, The Fourth Medical College of Peking University, Beijing, People's Republic of China.

Background: BCOR-CCNB3 sarcoma (BCS) is a group of undifferentiated small round cell sarcomas harboring the BCOR gene rearrangement which shares morphology with the Ewing sarcoma family as well as other malignant round blue cell tumors, thus making them difficult to diagnose. The aim of this study was to explore the role of molecular techniques in the diagnosis of BCS.

Methods: Twenty-three cases of EWSR1 rearrangement-negative undifferentiated small round cell sarcomas (Ewing-like sarcoma) were analyzed for the presence of BCOR gene rearrangement by Fluorescence in situ hybridization (FISH) and Reverse Transcription -Polymerase Chain Reaction (RT-PCR). The clinicopathological features of the positive cases were also reviewed. Fifteen additional cases were used as negative controls.

Results: Eight cases were found with BCOR gene rearrangement by FISH and reappraised as BCS. The patients ranged in age from 8 to 20 years old, with a male predominance (M:F = 6:2). All tumors were located in the lower extremities. The tumor locations were more common in bone (n = 6) than deep soft tissue (n = 2). Histologically, 7 of 8 tumors were predominately composed of spindle or ovoid cells. The tumor cells were usually arranged in solid hypercellular sheets without a distinct architectural pattern. IHC showed expression of TLE1 (100%), CCNB3 (88%), BCOR (71%). RT-PCR for BCOR-CCNB3 fusion transcript was positive in 7 of 8 cases. Pre-operative chemotherapy resulted in eradication of tumors in 5 patients after a follow-up of 7 to 42 months.

Conclusions: Efficient diagnosis of BCOR rearranged sarcomas is achieved by the using a combination of FISH and RT-PCR assays.
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http://dx.doi.org/10.1186/s13000-021-01114-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185946PMC
June 2021

Unique structure of active platinum-bismuth site for oxidation of carbon monoxide.

Nat Commun 2021 Jun 7;12(1):3342. Epub 2021 Jun 7.

Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.

As the technology development, the future advanced combustion engines must be designed to perform at a low temperature. Thus, it is a great challenge to synthesize high active and stable catalysts to resolve exhaust below 100 °C. Here, we report that bismuth as a dopant is added to form platinum-bismuth cluster on silica for CO oxidation. The highly reducible oxygen species provided by surface metal-oxide (M-O) interface could be activated by CO at low temperature (~50 °C) with a high CO production rate of 487 μmol·g·s at 110 °C. Experiment data combined with density functional calculation (DFT) results demonstrate that Pt cluster with surface Pt-O-Bi structure is the active site for CO oxidation via providing moderate CO adsorption and activating CO molecules with electron transformation between platinum atom and carbon monoxide. These findings provide a unique and general approach towards design of potential excellent performance catalysts for redox reaction.
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http://dx.doi.org/10.1038/s41467-021-23696-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184822PMC
June 2021

Association between doctor-patient familiarity and patient-centred care during general practitioner's consultations: a direct observational study in Chinese primary care practice.

BMC Fam Pract 2021 May 28;22(1):107. Epub 2021 May 28.

Department of Health Policy and Management, School of Public Health, Sun Yat-Sen University, Guangzhou, 510080, Guangdong, China.

Background: Patient-centred care is a core attribute of primary care. Not much is known about the relationship between patient-centred care and doctor-patient familiarity. This study aimed to explore the association between general practitioner (GP) perceived doctor-patient familiarity and the provision of patient-centred care during GP consultations.

Methods: This is a direct observational study conducted in eight community health centres in China. Level of familiarity was rated by GPs using a dichotomized variable (Yes/No). The provision of patient-centred care during GP consultations was measured by coding audiotapes using a modified Davis Observation Code (DOC) interactional instrument. Eight individual codes in the modified DOC were selected for measuring the provision of patient-centred care, including 'family information', 'treatment effects', 'nutrition guidance', 'exercise guidance', 'health knowledge', 'patient question', 'chatting', and 'counseling'. Multivariate analyses of covariance were adopted to evaluate the association between GP perceived doctor-patient familiarity and patient-centred care.

Results: A total of 445 audiotaped consultations were collected, with 243 in the familiar group and 202 in the unfamiliar group. No significant difference was detected in overall patient-centred care between the two groups. For components of patient-centred care, the number of intervals (1.36 vs 0.88, p = 0.026) and time length (7.26 vs. 4.40 s, p = 0.030) that GPs spent in 'health knowledge', as well as time length (13.0 vs. 8.34 s, p = 0.019) spent in 'patient question' were significantly higher in unfamiliar group. The percentage of 'chatting' (11.9% vs. 7.34%, p = 0.012) was significantly higher in the familiar group.

Conclusions: This study suggested that GP perceived doctor-patient familiarity may not be associated with GPs' provision of patient-centred care during consultations in the context of China. Not unexpectedly, patients would show more health knowledge and ask more questions when GPs were not familiar with them. Further research is needed to confirm and expand on these findings.
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http://dx.doi.org/10.1186/s12875-021-01446-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161971PMC
May 2021

Bromine-Substitution-Induced High- Two-Dimensional Bilayered Perovskite Photoferroelectric.

J Am Chem Soc 2021 May 17;143(20):7593-7598. Epub 2021 May 17.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, People's Republic of China.

High-Curie-temperature () ferroelectrics have exhibited broad applications in optoelectronic devices. Recently, two-dimensional multilayered perovskite ferroelectrics with excellent photoelectric attributes are attracting increasing interest as new systems of photoferroelectrics. However, the effective tuning of the value of a multilayered perovskite photoferroelectric system still remains a huge challenge. Here, by a halogen substitution strategy to introduce bromine atoms on -propylamine cations, the hybrid perovskite photoferroelectric (3-bromopropylaminium)(formamidinium)PbBr () with a high value (348.5 K) was obtained. It is notable that adopts a two-dimensional bilayered inorganic framework, with tight linking to the organic cation by C-Br···Br-Pb halogen···halogen interactions and N-H···Br hydrogen bonds. Intriguingly, in comparison with the prototypical compound (-propylaminium)(formamidinium)PbBr, a remarkable augmentation of 85.2 K in the resulting value of is clearly observed, which further broadens the temperature range of its application. In combination with the remarkable ferroelectric and semiconducting attributes, the reversible bulk photovoltaic effect was realized in single crystals of . This finding can not only enhance the hybrid perovskite ferroelectric family but also further promote the photoelectric application of ferroelectrics.
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http://dx.doi.org/10.1021/jacs.1c00459DOI Listing
May 2021

Sulfonic-Group-Grafted TiCT MXene: A Silver Bullet to Settle the Instability of Polyaniline toward High-Performance Zn-Ion Batteries.

ACS Nano 2021 May 29;15(5):9065-9075. Epub 2021 Apr 29.

College of Materials Science and Engineering, Shenzhen University, Shenzhen 518055, China.

Polyaniline (PANI) is a promising cathode material for Zn-ion batteries (ZIBs) due to its intrinsic conductivity and redox activity; however, the achievements of PANI in high-performance ZIBs are largely hindered by its instability during the repeated charge/discharge. Taking advantage of the high conductivity, flexibility, and grafting ability together, a surface-engineered TiCT MXene is designed as a silver bullet to fight against the deprotonation and swelling/shrinking issues occurring in the redox process of PANI, which are the origins of its instability. Specifically, the sulfonic-group-grafted TiCT(S-TiCT) continuously provides protons to improve the protonation degree of PANI and maintains the polymer backbone at a locally low pH, which effectively inhibits deprotonation and brings high redox activity along with good reversibility. Meanwhile, the conductive and flexible natures of S-TiCT assist the fast redox reaction of PANI and concurrently buffer its corresponding swelling/shrinking. Therefore, the S-TiCT-enhanced PANI cathode simultaneously achieves a high discharge capacity of 262 mAh g at 0.5 A g, a superior rate capability of 160 mAh g at 15 A g, and a good cyclability over 5000 cycles with 100% coulombic efficiency. This work enlightens the development of versatile MXene surface engineering for advanced batteries.
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http://dx.doi.org/10.1021/acsnano.1c02215DOI Listing
May 2021

Improving Ki67 Assessment Concordance with AI-Empowered Microscope: A Multi-institutional Ring Study.

Histopathology 2021 Apr 11. Epub 2021 Apr 11.

Department of Pathology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Purpose: The nuclear proliferation biomarker Ki67 plays potential prognostic and predictive roles in breast cancer treatment. However, lack of inter-pathologist consistency in Ki67 assessment limits the clinical use of Ki67. This paper reports a solution utilizing an artificial intelligence (AI)-empowered microscope to improve Ki67 scoring concordance.

Methods: We developed an AI empowered microscope where the conventional microscope was equipped with AI algorithms and AI results were provided to pathologists in real time through augmented reality. We recruited 30 pathologists with various experience levels from 5 institutes to assess Ki67 label index on 100 Ki67 stained slides for invasive breast cancer patients. In the first round, pathologists conducted visual assessment on a conventional microscope; in the second round, they were assisted with reference cards; and in the third round, they were assisted with an AI-empowered microscope.

Results: Experienced pathologists had better reproducibility and accuracy (ICC = 0.864, mean error = 8.25%) than inexperienced pathologists (ICC = 0.807, mean error = 11.0%) in visual assessment. Moreover, with reference cards, inexperienced (ICC = 0.836, mean error = 10.7%) and experienced pathologists (ICC = 0.875, mean error = 7.56%) improved their reproducibility and accuracy. Finally, both experienced (ICC = 0.937, mean error = 4.36%) and inexperienced pathologists (ICC = 0.923, mean error = 4.71%) improved the reproducibility and accuracy significantly with the AI-empowered microscope.

Conclusion: AI-empowered microscope allows seamless integration of the AI solution into the clinical workflow and helps pathologists to obtain a higher consistency and accuracy for the Ki67 assessment.
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http://dx.doi.org/10.1111/his.14383DOI Listing
April 2021

Construction of the optimization prognostic model based on differentially expressed immune genes of lung adenocarcinoma.

BMC Cancer 2021 Mar 1;21(1):213. Epub 2021 Mar 1.

Department of Oncology, Tumor Hospital of Shaanxi Province, Xi'an, 710061, People's Republic of China.

Background: Lung adenocarcinoma (LUAD) is the most common pathology subtype of lung cancer. In recent years, immunotherapy, targeted therapy and chemotherapeutics conferred a certain curative effects. However, the effect and prognosis of LUAD patients are different, and the efficacy of existing LUAD risk prediction models is unsatisfactory.

Methods: The Cancer Genome Atlas (TCGA) LUAD dataset was downloaded. The differentially expressed immune genes (DEIGs) were analyzed with edgeR and DESeq2. The prognostic DEIGs were identified by COX regression. Protein-protein interaction (PPI) network was inferred by STRING using prognostic DEIGs with p value< 0.05. The prognostic model based on DEIGs was established using Lasso regression. Immunohistochemistry was used to assess the expression of FERMT2, FKBP3, SMAD9, GATA2, and ITIH4 in 30 cases of LUAD tissues.

Results: In total,1654 DEIGs were identified, of which 436 genes were prognostic. Gene functional enrichment analysis indicated that the DEIGs were involved in inflammatory pathways. We constructed 4 models using DEIGs. Finally, model 4, which was constructed using the 436 DEIGs performed the best in prognostic predictions, the receiver operating characteristic curve (ROC) was 0.824 for 3 years, 0.838 for 5 years, 0.834 for 10 years. High levels of FERMT2, FKBP3 and low levels of SMAD9, GATA2, ITIH4 expression are related to the poor overall survival in LUAD (p < 0.05). The prognostic model based on DEIGs reflected infiltration by immune cells.

Conclusions: In our study, we built an optimal prognostic signature for LUAD using DEIGs and verified the expression of selected genes in LUAD. Our result suggests immune signature can be harnessed to obtain prognostic insights.
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http://dx.doi.org/10.1186/s12885-021-07911-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923649PMC
March 2021

Physical mapping of repetitive oligonucleotides facilitates the establishment of a genome map-based karyotype to identify chromosomal variations in peanut.

BMC Plant Biol 2021 Feb 20;21(1):107. Epub 2021 Feb 20.

Henan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002, Henan, China.

Background: Chromosomal variants play important roles in crop breeding and genetic research. The development of single-stranded oligonucleotide (oligo) probes simplifies the process of fluorescence in situ hybridization (FISH) and facilitates chromosomal identification in many species. Genome sequencing provides rich resources for the development of oligo probes. However, little progress has been made in peanut due to the lack of efficient chromosomal markers. Until now, the identification of chromosomal variants in peanut has remained a challenge.

Results: A total of 114 new oligo probes were developed based on the genome-wide tandem repeats (TRs) identified from the reference sequences of the peanut variety Tifrunner (AABB, 2n = 4x = 40) and the diploid species Arachis ipaensis (BB, 2n = 2x = 20). These oligo probes were classified into 28 types based on their positions and overlapping signals in chromosomes. For each type, a representative oligo was selected and modified with green fluorescein 6-carboxyfluorescein (FAM) or red fluorescein 6-carboxytetramethylrhodamine (TAMRA). Two cocktails, Multiplex #3 and Multiplex #4, were developed by pooling the fluorophore conjugated probes. Multiplex #3 included FAM-modified oligo TIF-439, oligo TIF-185-1, oligo TIF-134-3 and oligo TIF-165. Multiplex #4 included TAMRA-modified oligo Ipa-1162, oligo Ipa-1137, oligo DP-1 and oligo DP-5. Each cocktail enabled the establishment of a genome map-based karyotype after sequential FISH/genomic in situ hybridization (GISH) and in silico mapping. Furthermore, we identified 14 chromosomal variants of the peanut induced by radiation exposure. A total of 28 representative probes were further chromosomally mapped onto the new karyotype. Among the probes, eight were mapped in the secondary constrictions, intercalary and terminal regions; four were B genome-specific; one was chromosome-specific; and the remaining 15 were extensively mapped in the pericentric regions of the chromosomes.

Conclusions: The development of new oligo probes provides an effective set of tools which can be used to distinguish the various chromosomes of the peanut. Physical mapping by FISH reveals the genomic organization of repetitive oligos in peanut chromosomes. A genome map-based karyotype was established and used for the identification of chromosome variations in peanut following comparisons with their reference sequence positions.
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http://dx.doi.org/10.1186/s12870-021-02875-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896385PMC
February 2021

Dysregulation of Wnt/β-catenin signaling by protein kinases in hepatocellular carcinoma and its therapeutic application.

Cancer Sci 2021 May 6;112(5):1695-1706. Epub 2021 Apr 6.

Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Engineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Department of Hepatology, Institute of Hepatology and Metabolic Diseases, Institute of Integrated Chinese and Western Medicine for Oncology, The Affiliated Hospital of Hangzhou Normal University, College of Pharmacy, School of Medicine, Hangzhou Normal University, Hangzhou, China.

Wnt/β-catenin signaling is indispensable for many biological processes, including embryonic development, cell cycle, inflammation, and carcinogenesis. Aberrant activation of the Wnt/β-catenin signaling can promote tumorigenicity and enhance metastatic potential in hepatocellular carcinoma (HCC). Targeting this pathway is a new opportunity for precise medicine for HCC. However, inhibiting Wnt/β-catenin signaling alone is unlikely to significantly improve HCC patient outcome due to the lack of specific inhibitors and the complexity of this pathway. Combination with other therapies will be an important next step in improving the efficacy of Wnt/β-catenin signaling inhibitors. Protein kinases play a key and evolutionarily conserved role in the Wnt/β-catenin signaling and have become one of the most important drug targets in cancer. Targeting Wnt/β-catenin signaling and its regulatory kinase together will be a promising HCC management strategy. In this review, we summarize the kinases that modulate the Wnt/β-catenin signaling in HCC and briefly discuss their molecular mechanisms. Furthermore, we list some small molecules that target the kinases and may inhibit Wnt/β-catenin signaling, to offer new perspectives for preclinical and clinical HCC studies.
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http://dx.doi.org/10.1111/cas.14861DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088956PMC
May 2021

Antimicrobial Activity of Lemongrass Essential Oil () and Its Active Component Citral Against Dual-Species Biofilms of and Species.

Front Cell Infect Microbiol 2020 22;10:603858. Epub 2020 Dec 22.

Microbiome Laboratory, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, China.

Compared to mono-species biofilm, biofilms formed by cross-kingdom pathogens are more refractory to conventional antibiotics, thus complicating clinical treatment and causing significant morbidity. Lemongrass essential oil and its bioactive component citral were previously demonstrated to possess strong antimicrobial efficacy against pathogenic bacteria and fungi. However, their effects on polymicrobial biofilms remain to be determined. In this study, the efficacy of lemongrass () essential oil and its bioactive part citral against dual-species biofilms formed by and species was evaluated . Biofilm staining and viability test showed both lemongrass essential oil and citral were able to reduce biofilm biomass and cell viability of each species in the biofilm. Microscopic examinations showed these agents interfered with adhesive characteristics of each species and disrupted biofilm matrix through counteracting nucleic acids, proteins and carbohydrates in the biofilm. Moreover, transcriptional analyses indicated citral downregulated hyphal adhesins and virulent factors of , while also reducing expression of genes involved in quorum sensing, peptidoglycan and fatty acids biosynthesis of . Taken together, our results demonstrate the potential of lemongrass essential oil and citral as promising agents against polymicrobial biofilms as well as the underlying mechanisms of their activity in this setting.
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http://dx.doi.org/10.3389/fcimb.2020.603858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783362PMC
December 2020

Facial Sporotrichosis with Liver Cirrhosis Detected by Calcofluor White Treated with Itraconazole.

Mycopathologia 2021 Mar 3;186(1):141-142. Epub 2021 Jan 3.

Department of Dermatovenereology, West China Hospital, Sichuan University, No. 37, Guo Xue Xiang, Wuhou District, Chengdu, 610041, Sichuan, China.

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http://dx.doi.org/10.1007/s11046-020-00518-wDOI Listing
March 2021

Morphological Metaphor Mapping of Moral Concepts in Chinese Culture.

Front Psychol 2020 17;11:554061. Epub 2020 Dec 17.

School of Psychology, North China University of Science and Technology, Tangshan, China.

According to conceptual metaphor theory, individuals are thought to understand or express abstract concepts by using referents in the physical world-right and left for moral and immoral, for example. In this research, we used a modified Stroop paradigm to explore how abstract moral concepts are metaphorically translated onto physical referents in Chinese culture using the Chinese language. We presented Chinese characters related to moral and immoral abstract concepts in either non-distorted or distorted positions (Study 1) or rotated to the right or to the left (Study 2). When we asked participants to identify the Chinese characters, they more quickly and accurately identified morally positive characters if they were oriented upright or turned to the right and more quickly and accurately identified immoral characters when the characters were distorted or rotated left. These results support the idea that physical cues are used in metaphorically encoding social abstractions and moral norms and provided cross-cultural validation for conceptual metaphor theory, which would predict our results.
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http://dx.doi.org/10.3389/fpsyg.2020.554061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774603PMC
December 2020

Chiral Lead-Free Hybrid Perovskites for Self-Powered Circularly Polarized Light Detection.

Angew Chem Int Ed Engl 2021 Apr 3;60(15):8415-8418. Epub 2021 Mar 3.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, 350002, China.

Metal-halide perovskites are recently emerging as the promising alternative for CPL detection owing to their CPL-sensitive property induced by chiral organics and efficient charge transport of inorganic frameworks. However, most of these reported chiral perovskites involve high concentrations of toxic Pb which will become the potential bottleneck for their further application. Herein, we successfully developed two lead-free halide double perovskites, [(R)-β-MPA] AgBiI ((R)-β-MPA=(R)-(+)-β-methylphenethylammonium, 1-R), and [(S)-β-MPA] AgBiI ((S)-β-MPA=(S)-(-)-β-methylphenethylammonium, 1-S). Circular dichroism measurements reveal that these perovskites exhibit notable chirality induced by organic cations to distinguish different polarization states of CPL photons. Significantly, they present unique chiral polar photovoltaic, and resulting self-powered CPL detection without an external power source is unprecedentedly achieved. Furthermore, an anisotropy factor up to 0.3 is acquired for the self-powered CPL detection, reaching the highest value among reported chiral perovskites. This work suggests hybrid double perovskites are promising photoelectronic candidates, and provides a new approach for exploring new "green" circularly polarized light-sensitive materials with high performance.
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http://dx.doi.org/10.1002/anie.202013947DOI Listing
April 2021

Morphology, morphogenesis and molecular phylogeny of a novel soil ciliate, Afrokahliella paramacrostoma n. sp. (Ciliophora, Hypotrichia).

Eur J Protistol 2021 Feb 10;77:125748. Epub 2020 Nov 10.

Laboratory of Protozoological Biodiversity and Evolution in Wetland, College of Life Sciences, Shaanxi Normal University, Xi'an 710119, PR China. Electronic address:

A soil hypotrich ciliate, Afrokahliella paramacrostoma n. sp., was discovered in China. Its morphology, morphogenesis and molecular phylogeny were investigated using standard methods. The new species is characterized as follows: body about 140-180 × 60-70 μm in vivo, cortical granules absent, contractile vacuole positioned about 40% down length of body, 5-9 macronuclear nodules, 34-49 adoral membranelles, 3-5 buccal and 3-6 parabuccal cirri, usually two frontoventral rows, three or four left and two or three right marginal rows, three dorsal kineties and one dorsomarginal kinety; 1-3 and one or two caudal cirri located at the ends of dorsal kineties 1 and 2, respectively. The ontogenetic process is characterized by: (1) the marginal anlagen on each side develop in the outer right and the inner left marginal rows, respectively; (2) five frontoventral-transverse cirral anlagen, anlagen II-IV develop in secondary mode; (3) dorsal morphogenesis follows a typical Urosomoida-pattern, no parental dorsal kineties are retained; (4) caudal cirri are generated at the ends of dorsal kineties 1 and 2. Phylogenetic analyses based on SSU rDNA sequence data reveals that Afrokahliella paramacrostoma n. sp. is closely related to Parakahliella macrostoma and Hemiurosomoida longa.
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http://dx.doi.org/10.1016/j.ejop.2020.125748DOI Listing
February 2021

Simply closing the reactor improves the electron efficiency of zerovalent iron towards various metal(loid)s removal.

Water Environ Res 2020 Nov 30. Epub 2020 Nov 30.

Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research Institute, Shanghai, 201204, China.

The controlled corrosion of zerovalent iron (ZVI) is crucial for the favorable performance of ZVI towards metal(loid)s removal, and dissolved oxygen (DO) plays an important role in the process of ZVI corrosion. However, few efforts have been made to control the concentration of DO in real practice. In this study, we found that the electron efficiency and the specific removal capacity of ZVI towards the removal of four metal(loid)s were increased by 1.2-9.1 times and 1.2-3.6 times, respectively, by simply closing the reactor, while the removal kinetics of metal(loid)s was slightly influenced. The rate constants obtained under open condition were always greater than those obtained under closed condition, and the removal amounts of metal(loid)s by ZVI at the reaction equilibrium under closed condition were nearly equivalent to those under open condition. Compared to the case under open condition, the consumption-redissolution process of DO was decelerated under closed condition, and the rapid corrosion of ZVI was alleviated subsequently. Although closing the reactor is simple, it does contribute much to the favorable electron efficiency of ZVI towards metal(loid)s sequestration and can be easily adopted in real practice.
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http://dx.doi.org/10.1002/wer.1487DOI Listing
November 2020

Exploring a Fatigue-Free Layered Hybrid Perovskite Ferroelectric for Photovoltaic Non-Volatile Memories.

Angew Chem Int Ed Engl 2021 May 11;60(19):10598-10602. Epub 2021 Mar 11.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, 350002, P. R. China.

Through a functional unit-transmutation strategy, a fatigue-free layered hybrid perovskite ferroelectric (C H CH NH ) CsPb Br (BCPB) has been developed, which demonstrates stable spontaneous polarization (P ) of 6.5 μC cm and high Curie temperature up to 425 K. Meanwhile, BCPB shows splendid bulk photovoltaic effect (BPVE) properties with noticeable zero-bias photocurrent density (5 μA cm ), and high on/off switching ratio of current (over 3×10 ); these merits even overmatch the most known ferroelectric semiconductor BiFeO . The unique structure with self-regulated net electrical charged layers gives rise to the fatigue-free feature of P and BPVE (no significant fatigue after 10 polarity switching cycles), promoting the potential applications of BCPB in photovoltaic non-volatile memories. This work offers an efficient approach for exploring fatigue-free semiconducting ferroelectrics as well as excavates their further applications in next-generation electronic devices.
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http://dx.doi.org/10.1002/anie.202012601DOI Listing
May 2021

Novel fluorescent nitrogen-doped carbon dots derived from Panax notoginseng for bioimaging and high selectivity detection of Cr.

Analyst 2021 Feb 25;146(3):911-919. Epub 2020 Nov 25.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.

Carbon dots (CDs) and photoluminescent carbon dots (Pn-CDs) are promising nanomaterials due to their bioimaging applications and have attracted considerable attention because of their excellent stability, good biocompatibility, and low biotoxicity. Here, the Pn-CDs and highly fluorescent nitrogen-doped CDs (Pn N-CDs) derived from Panax notoginseng were successfully synthesized by a simple hydrothermal method. Pn N-CDs exhibit optical properties and stability superior to those of Pn-CDs and can be better used as fluorescent dyes and probes in biological imaging. The obtained Pn N-CDs can be effectively applied to the imaging of bacteria, fungi, plant cells, and protozoa. In addition, Pn N-CDs can perform specific staining on the membranes of all tested cells. The in vivo imaging of mice revealed that Pn N-CDs exhibit nontoxicity and good biocompatibility and biodistribution. Furthermore, Pn N-CDs can be utilized as fluorescent probes for the rapid and highly selective detection of Cr. Hence, a simple, cost-effective, scalable, and green synthetic approach based on traditional Chinese medicine-derived CDs can be used to develop biolabeling, membrane targeting, and optical sensing probes.
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http://dx.doi.org/10.1039/d0an01599gDOI Listing
February 2021

Epidemiology and microbiology of Gram-negative bloodstream infections in a tertiary-care hospital in Beijing, China: a 9-year retrospective study.

Expert Rev Anti Infect Ther 2021 Jun 3;19(6):769-776. Epub 2020 Dec 3.

Department of Respiratory Medicine, Chinese PLA General Hospital, Beijing, China.

: Gram-negative bacterial bloodstream infections (BSIs) are associated with high morbidity and mortality. The present study examines the incidence, clinical characteristics, microbiological features, drug resistance and mortality associated with Gram-negative bacterial BSIs at a tertiary-care hospital in Beijing, China.: This retrospective cohort study of patients with Gram-negative bacterial BSIs was performed between 1 January 2010 and 31 December 2018 at the Chinese Peoples Liberation Army General Hospital.: A total of 6867 episodes of Gram-negative bacterial BSIs occurred among 3199 patients over 9 years. The overall incidence of Gram-negative bacterial BSIs fluctuated from 2.30 to 2.55 episodes per 1000 admissions over 9 years. was the major pathogen (34.3%). The antibiotic resistance of ESBLs-producing was higher than non-ESBLs producing including the majority of antibiotics, but to carbapenems (0.7% VS 5.1%). Between 2010 and 2018, the overall mortality of Gram-negative bacterial BSIs decreased from 11.41% to 9.05% (X = 6.95, P = 0.434).: Cephalosporins and carbapenem antibiotics were considered as the optimal treatment for patients with Gram-negative bacterial BSIs except for , which was treated according to the drug sensitivity or multidrug combination.
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http://dx.doi.org/10.1080/14787210.2021.1848544DOI Listing
June 2021

Acquiring High-T Layered Metal Halide Ferroelectrics via Cage-Confined Ethylamine Rotators.

Angew Chem Int Ed Engl 2021 Feb 16;60(6):2839-2843. Epub 2020 Dec 16.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences Institution, Fuzhou, Fujian, 350002, P. R. China.

Two-dimensional (2D) organic-inorganic hybrid perovskite (OIHP) ferroelectrics have attracted widespread interest in the field of optoelectronics due to the combination of excellent semiconducting and ferroelectric properties. The Curie temperature (T ), below which ferroelectricity exists, is a crucial parameter for ferroelectrics. However, the lack of research on T tuning of 2D OIHP ferroelectrics hinders their further progress. Here, through incorporating ethylammonium (EA) as cage-confined rotators, we obtained two 2D OIHP ferroelectrics, (IBA) (EA)Pb Br (2L; IBA=isobutylammonium), and (IBA) (EA) Pb Br (3L). Intriguingly, T is successfully tuned from 326 K (2L) to 370 K (3L) with increasing layer thickness. Structural and computational analyses suggest that the improvement of T is due to the higher phase-transition energy barrier triggered by the cage-confined EA rotators with increased layer thickness. This work suggests that EA is an effective "cage-confined rotator" to rationally design high-T 2D OIHP ferroelectrics.
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http://dx.doi.org/10.1002/anie.202011270DOI Listing
February 2021

The Time Course of Perceptual Closure of Incomplete Visual Objects: An Event-Related Potential Study.

Comput Intell Neurosci 2020 6;2020:8825197. Epub 2020 Oct 6.

Key Laboratory of Complex System Control Theory and Application, Tianjin University of Technology, Tianjin 300384, China.

Perceptual organization is an important part of visual and auditory information processing. In the case of visual occlusion, whether the loss of information in images could be recovered and thus perceptually closed affects object recognition. In particular, many elderly subjects have defects in object recognition ability, which may be closely related to the abnormalities of perceptual functions. This phenomenon even can be observed in the early stage of dementia. Therefore, studying the neural mechanism of perceptual closure and its relationship with sensory and cognitive processing is important for understanding how the human brain recognizes objects, inspiring the development of neuromorphic intelligent algorithms of object recognition. In this study, a new experiment was designed to explore the realistic process of perceptual closure under occlusion and intact conditions of faces and building. The analysis of the differences in ERP components P1, N1, and Ncl indicated that the subjective awareness of perceptual closure mainly occurs in Ncl, but incomplete information has been processed and showed different manners compared to complete stimuli in N170 for facial materials. Although occluded, faces, but not buildings, still maintain the specificity of perceptual processing. The Ncl by faces and buildings did not show significant differences in both amplitude and latency, suggesting a "completing" process regardless of categorical features.
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http://dx.doi.org/10.1155/2020/8825197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559784PMC
October 2020

Erianin inhibits the oncogenic properties of hepatocellular carcinoma via inducing DNA damage and aberrant mitosis.

Biochem Pharmacol 2020 12 6;182:114266. Epub 2020 Oct 6.

College of Pharmacy, School of Medicine, Department of Hepatology, Institute of Hepatology and Metabolic Diseases, Institute of Integrated Chinese and Western Medicine for Oncology, the Affiliated Hospital of Hangzhou Normal University, Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines, Engineering Laboratory of Development and Application of Traditional Chinese Medicines, Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province, Hangzhou Normal University, Hangzhou, Zhejiang 311121, China; Laboratory of Cancer Genomics, Division of Cellular and Molecular Research, National Cancer Centre Singapore, Singapore 169610, Singapore. Electronic address:

Natural compounds have been confirmed as one of the most feasible solutions for hard-to-treat cancers such as hepatocellular carcinoma (HCC). Erianin, a natural bibenzyl compound from Dendrobium chrysotoxum, has been recently discovered with anticancer property in cancer cells. However, the roles and the molecular mechanisms of erianin in HCC remain unknown. The present study evaluates the effect of erianin on human HCC cells by inhibiting cell proliferation, inducing apoptotic-related cell death and hampering tumorigenicity. Furthermore, it was found that erianin could cause irreparable DNA damage, induce G2/M arrest and deregulate mitotic regulators. It was also observed that many cells with damaged DNA induced by erianin could overcome G2/M arrest and enter mitosis, leading to abnormal mitosis, and subsequently mitotic catastrophe and apoptotic-related cell death. The present study confirmed that erianin could be a potential antitumor agent for HCC clinical treatment.
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http://dx.doi.org/10.1016/j.bcp.2020.114266DOI Listing
December 2020

Chromatin modifier MTA1 regulates mitotic transition and tumorigenesis by orchestrating mitotic mRNA processing.

Nat Commun 2020 09 8;11(1):4455. Epub 2020 Sep 8.

State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.

Dysregulated alternative splicing (AS) driving carcinogenetic mitosis remains poorly understood. Here, we demonstrate that cancer metastasis-associated antigen 1 (MTA1), a well-known oncogenic chromatin modifier, broadly interacts and co-expresses with RBPs across cancers, contributing to cancerous mitosis-related AS. Using developed fCLIP-seq technology, we show that MTA1 binds abundant transcripts, preferentially at splicing-responsible motifs, influencing the abundance and AS pattern of target transcripts. MTA1 regulates the mRNA level and guides the AS of a series of mitosis regulators. MTA1 deletion abrogated the dynamic AS switches of variants for ATRX and MYBL2 at mitotic stage, which are relevant to mitosis-related tumorigenesis. MTA1 dysfunction causes defective mitotic arrest, leads to aberrant chromosome segregation, and results in chromosomal instability (CIN), eventually contributing to tumorigenesis. Currently, little is known about the RNA splicing during mitosis; here, we uncover that MTA1 binds transcripts and orchestrates dynamic splicing of mitosis regulators in tumorigenesis.
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http://dx.doi.org/10.1038/s41467-020-18259-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479136PMC
September 2020

Cancer metastasis-associated protein 1 localizes to the nucleolus and regulates pre-rRNA synthesis in cancer cells.

J Cell Biochem 2021 Feb 12;122(2):180-188. Epub 2020 Aug 12.

State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.

Metastasis-associated protein 1 (MTA1) is a critical component of the nucleosome remodeling and histone deacetylase (NuRD) complex. MTA1 has several biological functions, and it is closely associated with the malignant properties of human cancers; however, the mechanisms and subcellular localization of MTA1 in cells remain unclear. Some initial studies indicated that MTA1 was absent from the nucleolus; however, several NuRD components were recently found to be present in the nucleolus, where they regulate preribosomal RNA (pre-rRNA) transcription. In this study, we demonstrated that MTA1 is definitely localized to the nucleolus and regulates pre-rRNA transcription, which is consistent with the recent reports on NuRD. To determine if MTA1 was present in the nucleolus, we utilized the following complementary molecular approaches: immunofluorescence, GFP-tag tracking, immunoelectron microscopy, and immunoprecipitation (IP). To examine the role of MTA1 in rRNA synthesis, we performed quantitative polymerase chain reaction analysis. We revealed that both endogenous and exogenous MTA1 showed apparent granule-like nucleolar subcellular localization. MTA1 interacts with two major resident nucleolar proteins, nucleolin and nucleophosmin. Immunofluorescent colocalization analyses showed that MTA1 localizes to the fibrillarin-deficient regions of the nucleolus, and Co-IP experiments indicated that there was no interaction between MTA1 and fibrillarin; further, fibrillarin was not identified in the MTA1 interactome. Loss- and gain-of-function studies indicated that MTA1 promotes pre-rRNA transcription in cancer cells. Collectively, our data identify MTA1 as a novel nucleolar protein, and activation of pre-rRNA transcription in cancer cells may be an alternative mechanism by which MTA1 promotes malignancies.
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http://dx.doi.org/10.1002/jcb.29837DOI Listing
February 2021

[Down-regulation of HOTAIR Reverses the Resistance of Gefitinib in HCC827 Cells by Increasing the Expression of PTEN].

Zhongguo Fei Ai Za Zhi 2020 Sep 10;23(9):762-771. Epub 2020 Aug 10.

Department of Oncology, Tumor hospital of Shaanxi Province, Xi'an 710061, China.

Background: Lung cancer is the most common cancer worldwide with the highest morbidity and mortality, in which the non-small cell lung cancer accounts for 80% of all cases. The expression of (HOX transcript antisense RNA) HOTAIR were abnormal in a variety of tumor tissues and is involved in the regulation of the occurrence and development of lung cancer. The purpose of this study is to investigate the effect and mechanism of down-regulation of HOTAIR on gefitinib resistance of lung adenocarcinoma HCC827 cells by targeting PTEN.

Methods: The HOTAIR downstream target gene was predicted by bioinformatics database. The small interfering RNAs (siRNA) which is corresponding to HOTAIR was transfected using Lipofectamine™ 2000. Quantitative real-time PCR (RT-qPCR) and Western blot were used to detect the expression of HOTAIR, PTEN, PI3K and AKT in HCC827 and HCC827GR cells. MTT assay was used to detect the changes in drug resistance of HCC827GR cells. Flow cytometry analysis were used to test the cell proliferation and the rate of apoptosis.

Results: The expression of HOTAIR increased in HCC827GR and the serum of NSCLC patients with gefitinib resistance (P<0.05). Transfection of HOTAIR siRNA decreased the expression of HOTAIR (P<0.05), and increased the expressions of PTEN (P<0.05), while the expression of PI3K and AKT were decreased (P<0.05). Compared with the blank control group, down-regulation of HOTAIR increased the sensitivity of HCC827GR cells to gefitinib. The cell proliferation ability was decreased and the apoptosis was promoted apparently (P<0.05).

Conclusions: Down-regulation of HOTAIR can suppress the cell growth and promote the apoptosis, and it can reverse the resistance of HCC827GR cells to gefitinib. Its potential mechanism may be related with the targeting of PTEN/PI3K/AKT pathway.
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http://dx.doi.org/10.3779/j.issn.1009-3419.2020.103.13DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7519959PMC
September 2020

Retrospective Observation of Low-Dose Rituximab Treatment in Chinese Patients With Neuromyelitis Optica Spectrum Disorders in a Real-World Setting.

Front Neurol 2020 7;11:642. Epub 2020 Jul 7.

Neurology, Department of Medicine, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China.

This study aimed to investigate the efficacy and safety of low-dose rituximab (RTX) in the treatment of neuromyelitis optica spectrum disorders (NMOSD) patients. NMOSD patients were treated with RTX at ~25% of the standard dose. The annualized relapse rate (ARR), expanded disability status scale (EDSS) score, visual function system scale (VFSS) and length of spinal cord lesions before and after treatment were statistically compared. The dynamic changes in the proportion of CD19 B lymphocytes after treatment were monitored, and adverse reactions were recorded. In total, 36 NMOSD patients who received a low-dose RTX treatment (375-mg/m induction dose and 500 mg every 6 months) were recruited. The mean follow-up time after the RTX treatment was 19.83 ± 7.74 months. After the treatment, the ARR decreased from 1.97 ± 1.93 to 0.12 ± 0.32, the EDSS score decreased from 3.43 ± 1.49 to 3.10 ± 1.88, and the spinal cord lesion length decreased from 5.54 ± 3.96 to 4.31 ± 3.73. These differences were all statistically significant. The subgroup analysis of the patients who had previously received non-steroidal immunosuppressants (NSISs) ( = 20) showed that after the RTX treatment, the ARR decreased from 0.66 ± 0.51 to 0.08 ± 0.26, the EDSS score decreased from 3.65 ± 1.22 to 3.40 ± 1.99, and the spinal cord lesion length decreased from 5.68 ± 3.73 to 4.21 ± 3.58. These differences were all statistically significant. The VFSS scores did not show a significant change. The Kaplan-Meier analysis showed that low-dose RTX significantly delayed recurrence, which was also observed in the subgroup analysis of patients who previously received NSISs. Five relapses in 5 cases were noted after the low-dose RTX administration, and the percentage of CD19 B cells remained < 1% in 3 cases during relapse. During the RTX treatment and subsequent follow-up, 8 (22.2%) patients reported adverse reactions, all of which were minor. Low-dose RTX is an effective and safe treatment method for NMOSDs. This method is worth popularizing in developing countries or regions, especially in areas where RTX is not covered by medical insurance.
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http://dx.doi.org/10.3389/fneur.2020.00642DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358348PMC
July 2020

Ferroelectricity-Driven Self-Powered Ultraviolet Photodetection with Strong Polarization Sensitivity in a Two-Dimensional Halide Hybrid Perovskite.

Angew Chem Int Ed Engl 2020 Oct 26;59(43):18933-18937. Epub 2020 Aug 26.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, 350002, P. R. China.

Polarization-sensitive ultraviolet (UV) photodetection is highly indispensable in military and civilian applications and has been demonstrated with various wide-band photodetectors. However, it still remains elusive to achieve the self-powered devices, which can be operated in the absence of external bias. Herein, for the first time, ferroelectricity-driven self-powered photodetection towards polarized UV light was successfully demonstrated in a 2D wide-band gap hybrid ferroelectric (BPA) PbBr (BPA=3-bromopropylammonium) (1). We found that the prominent spontaneous polarization in 1 results in a bulk photovoltaic effect (BPVE) of 0.85 V, that independently drives photoexcited carriers separation and transport and thus supports self-powered ability. This self-powered detector shows strong polarization sensitivity to linearly polarized UV illumination with a polarization ratio up to 6.8, which is superior to that of previously reported UV-polarized photodetectors (ZnO, GaN, and GeS ).
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http://dx.doi.org/10.1002/anie.202005092DOI Listing
October 2020

Long noncoding RNA PRKCQ-AS1 promotes CRC cell proliferation and migration via modulating miR-1287-5p/YBX1 axis.

J Cell Biochem 2020 10 3;121(10):4166-4175. Epub 2020 Jul 3.

Department of Internal Medicine, Shaanxi Tumour Hospital, Xi'an, Shaanxi, China.

Colorectal cancer (CRC) brings more than 600 000 deaths every year around the globe, making itself the third most frequently occurred carcinoma. The great progress human achieved in diagnosis and treatment of various cancers has failed to reverse this trend. Fortunately, growing evidence has implied the relationship between lncRNAs and cancer progression. Long noncoding RNA (lncRNA) PRKCQ-AS1 was heightened in CRC cells and tissues and related with dismal prognosis of CRC patients. Knockdown of PRKCQ-AS1 would induce a decrease in proliferative and migrating ability of CRC cells. Also, PRKCQ-AS1 enriched in cytoplasm of CRC cells and negatively regulated miR-1287-5p level. More important, PRKCQ-AS1 could bind to argonaute 2 and function in the RNA-induced silencing complex with miR-1287-5p. Therefore, PRKCQ-AS1 was a competing endogenous RNA for miR-1287-5p. Subsequently, it was validated that miR-1287-5p could suppress the proliferative and migratory functions in CRC. Furthermore, PRKCQ-AS1 could upregulate the mRNA and protein level of YBX1 targeted by miR-1287-5p. And YBX1 expression was elevated in CRC cells and tissues. Rescue assays in vitro and in vivo showed that overexpression of YBX1 could partly offset the effect of CRC progression induced by knocking down PRKCQ-AS1, demonstrating PRKCQ-AS1 mediating CRC progression via miR-1287-5p/YBX1 pathway.
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http://dx.doi.org/10.1002/jcb.29712DOI Listing
October 2020

Refractory lower urinary tract symptoms in patients with lumbar disc hernia relieved by non-surgical treatment.

World J Urol 2021 May 1;39(5):1597-1605. Epub 2020 Jul 1.

Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, People's Republic of China.

Purpose: Refractory lower urinary tract symptoms (LUTS) coexisting with lumbar disc hernia (LDH) have been shown to resolve following LDH surgery, implying that LDH causes these LUTS. The purpose of this study was to report outcomes in patients with refractory LUTS and LDH following non-surgical treatment targeting LDH.

Methods: A retrospective cohort study was conducted using outpatient data collected at Tongji Hospital, China, between 2016 and 2018. This study included 131 adult patients with refractory LUTS and LDH. Patients were stratified into two groups. Group A underwent non-surgical treatment for LDH plus pharmacological treatment for LUTS. Group B underwent only pharmacological treatment for LUTS. The International Prostate Symptom Score (IPSS), the IPSS quality of life (QoL) score, and uroflowmetry were used to evaluate outcomes.

Results: In group A, following treatment, the maximum flow rate (Qmax) increased by 3.92 ml/s (p < 0.001), the IPSS reduced by 5.99 points (p < 0.001), and the QoL score decreased by 1.51 points (p < 0.001). In group B, the Qmax increased by 0.09 ml/s (p = 0.833), the IPSS reduced by 0.72 points (p = 0.163), and the QoL score decreased by 0.07 points (p = 0.784).

Conclusions: LUTS can be relieved by a combination of pharmacological treatment for LUTS and non-surgical treatment for LDH in some refractory LUTS patients with LDH. MRI is recommended for these patients.
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http://dx.doi.org/10.1007/s00345-020-03330-9DOI Listing
May 2021

HLY78 protects blood-brain barrier integrity through Wnt/β-catenin signaling pathway following subarachnoid hemorrhage in rats.

Brain Res Bull 2020 09 18;162:107-114. Epub 2020 Jun 18.

Department of Neurosurgery, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China. Electronic address:

Wnt/β-catenin signaling plays an essential role in blood-brain barrier (BBB) formation and maintenance under pathophysiological conditions. HLY78, a lycorine derivative, has been identified as a novel activator of Wnt/β-catenin signaling in vitro. However, the effects of HLY78 on the BBB function in subarachnoid hemorrhage (SAH) are not yet validated. The present study was designed to investigate the impacts of HLY78 on the BBB in an endovascular perforation induced SAH model of Sprague-Dawley rats. Western blot, immunofluorescence staining, neurological function, brain water content, and Evans blue assay were performed after SAH induction. The results revealed that the expression of phosphorylated low-density lipoprotein receptor-related protein 6 (p-LRP6) was significantly increased after SAH and further augmented by HLY78. Administration of HLY78 significantly improved neurobehavioral functions and attenuated BBB leakage following SAH. Moreover, HLY78 markedly increased the β-catenin expression followed with the up-regulation of Occludin, ZO-1, and Claudin-5 after SAH, which was reversed by LRP6 siRNA. In conclusion, HLY78 could preserve BBB integrity, possibly through the Wnt/β-catenin signaling pathway. HLY78 might be a potential treatment option to protect BBB integrity following SAH.
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http://dx.doi.org/10.1016/j.brainresbull.2020.06.003DOI Listing
September 2020

HLY78 Attenuates Neuronal Apoptosis via the LRP6/GSK3β/β-Catenin Signaling Pathway After Subarachnoid Hemorrhage in Rats.

Neurosci Bull 2020 Oct 20;36(10):1171-1181. Epub 2020 Jun 20.

Department of Neurosurgery, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, China.

Neuronal apoptosis is one of the essential mechanisms of early brain injury after subarachnoid hemorrhage (SAH). Recently, HLY78 has been shown to inhibit apoptosis in tumor cells and embryonic cells caused by carbon ion radiation through activation of the Wnt/β-catenin pathway. This study was designed to explore the anti-apoptotic role of HLY78 in experimental SAH. The results demonstrated that HLY78 attenuated neuronal apoptosis and the neurological deficits after SAH through the activation of low-density lipoprotein receptor-related protein 6 (LRP6), which subsequently increased the level of phosphorylated glycogen synthesis kinase 3 beta (p-GSK3β) (Ser9), β-catenin, and Bcl-2, accompanied by a decrease of p-β-catenin, Bax, and cleaved caspase 3. An LRP6 small-interfering ribonucleic acid reversed the effects of HLY78. In conclusion, HLY78 attenuates neuronal apoptosis and improves neurological deficits through the LRP6/GSK3β/β-catenin signaling pathway after SAH in rats. HLY78 is a promising therapeutic agent to attenuate early brain injury after SAH.
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http://dx.doi.org/10.1007/s12264-020-00532-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532258PMC
October 2020