Publications by authors named "Lina Huang"

125 Publications

A novel risk-predicted nomogram for sepsis associated-acute kidney injury among critically ill patients.

BMC Nephrol 2021 May 10;22(1):173. Epub 2021 May 10.

Department of President's Office, YouJiang Medical University for Nationalities, Baise, China.

Background: Acute kidney injury (AKI) is a prevalent and severe complication of sepsis contributing to high morbidity and mortality among critically ill patients. In this retrospective study, we develop a novel risk-predicted nomogram of sepsis associated-AKI (SA-AKI).

Methods: A total of 2,871 patients from the Medical Information Mart for Intensive Care III (MIMIC-III) critical care database were randomly assigned to primary (2,012 patients) and validation (859 patients) cohorts. A risk-predicted nomogram for SA-AKI was developed through multivariate logistic regression analysis in the primary cohort while the nomogram was evaluated in the validation cohort. Nomogram discrimination and calibration were assessed using C-index and calibration curves in the primary and external validation cohorts. The clinical utility of the final nomogram was evaluated using decision curve analysis.

Results: Risk predictors included in the prediction nomogram included length of stay in intensive care unit (LOS in ICU), baseline serum creatinine (SCr), glucose, anemia, and vasoactive drugs. Nomogram revealed moderate discrimination and calibration in estimating the risk of SA-AKI, with an unadjusted C-index of 0.752, 95 %Cl (0.730-0.774), and a bootstrap-corrected C index of 0.749. Application of the nomogram in the validation cohort provided moderate discrimination (C-index, 0.757 [95 % CI, 0.724-0.790]) and good calibration. Besides, the decision curve analysis (DCA) confirmed the clinical usefulness of the nomogram.

Conclusions: This study developed and validated an AKI risk prediction nomogram applied to critically ill patients with sepsis, which may help identify reasonable risk judgments and treatment strategies to a certain extent. Nevertheless, further verification using external data is essential to enhance its applicability in clinical practice.
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http://dx.doi.org/10.1186/s12882-021-02379-xDOI Listing
May 2021

Targeting Biofilm of MDR by Phages Using a Catheter Model.

Antibiotics (Basel) 2021 Apr 2;10(4). Epub 2021 Apr 2.

Institute of Dental Sciences, School of Dentistry, Hebrew University of Jerusalem, Jerusalem 91120, Israel.

spp. are emerging pathogens mainly in nosocomial infections. in particular is involved in urinary tract infections and contributes significantly to the high incidence of biofilm-formation in catheterized patients. Furthermore, recent reports suggested a role for multiple drug resistant (MDR) in hospital-associated outbreaks which leads to excessive complications resulting in challenging treatments. Phage therapy is currently one of the most promising solutions to combat antibiotic-resistant infections. However, the number of available phages targeting spp. is extremely limited, restricting the use of phage therapy in such cases. In the present study, we describe the isolation and characterization of 17 lytic and temperate bacteriophages targeting clinical isolates of spp. as part of the Israeli Phage Bank (IPB). These phages, isolated from sewage samples, were evaluated for host range activity and effectively eradicated 95% of the tested bacterial strains isolated from different geographic locations and displaying a wide range of antibiotic resistance. Their lytic activity is demonstrated on agar plates, planktonic cultures, and biofilm formed in a catheter model. The results suggest that these bacteriophages can potentially be used for treatment of antibiotic-resistant spp. infections in general and of urinary tract infections in particular.
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http://dx.doi.org/10.3390/antibiotics10040375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065852PMC
April 2021

Vibrio nitrifigilis sp. nov., a marine nitrogen-fixing bacterium isolated from the lagoon sediment of an islet inside an atoll.

Antonie Van Leeuwenhoek 2021 Apr 17. Epub 2021 Apr 17.

Institute of Oceanography, National Taiwan University, P.O. Box 23-13, Taipei, 10617, Taiwan.

A nitrogen-fixing isolate of facultatively anaerobic, marine bacterium, designated strain NFV-1, was recovered from the lagoon sediment of Dongsha Island, Taiwan. It was a Gram-negative rod which exhibited motility with monotrichous flagellation in broth cultures. The strain required NaCl for growth and grew optimally at about 25-35 °C, 3% NaCl and pH 7-8. It grew aerobically and could achieve anaerobic growth by fermenting D-glucose or other carbohydrates as substrates. NHCl could serve as a sole nitrogen source for growth aerobically and anaerobically, whereas growth with N as the sole nitrogen source was observed only under anaerobic conditions. Cellular fatty acids were predominated by C ω7c, C, and C ω7c. The major polar lipids consisted of phosphatidylethanolamine and phosphatidylserine. Strain NFV-1 had a DNA G + C content of 42.5 mol%, as evaluated according to the chromosomal DNA sequencing data. Analyses of sequence similarities and phylogeny based on the 16S rRNA genes, together with the housekeeping genes, gyrB, ftsZ, mreB, topA and gapA, indicated that the strain formed a distinct species-level lineage in the genus Vibrio of the family Vibrionaceae. These phylogenetic data and those from genomic and phenotypic characterisations support the establishment of a novel Vibrio species, for which the name Vibrio nitrifigilis sp. nov. (type strain NFV-1 = BCRC 81211 = JCM 33628) is proposed.
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http://dx.doi.org/10.1007/s10482-021-01567-xDOI Listing
April 2021

Ramatroban-Based Analogues Containing Fluorine Group as Potential F-Labeled Positron Emission Tomography (PET) G-Protein Coupled Receptor 44 (GPR44) Tracers.

Molecules 2021 Mar 6;26(5). Epub 2021 Mar 6.

Department of Translational Research & Cellular Therapeutics, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA.

Diabetes remains one of the fastest growing chronic diseases and is a leading source of morbidity and accelerated mortality in the world. Loss of beta cell mass (BCM) and decreased sensitivity to insulin underlie diabetes pathogenesis. Yet, the ability to safely and directly assess BCM in individuals with diabetes does not exist. Measures such as blood glucose provide only a crude indirect picture of beta cell health. PET imaging could, in theory, allow for safe, direct, and precise characterization of BCM. However, identification of beta cell-specific radiolabeled tracers remains elusive. G-protein coupled receptor 44 (GPR44) is a transmembrane protein that was characterized in 2012 as highly beta cell-specific within the insulin-positive islets of Langerhans. Accordingly, radiolabeling of existing GPR44 antagonists could be a viable method to accelerate PET tracer development. The present study aims to evaluate and summarize published analogues of the GPR44 antagonist ramatroban to develop F-labeled PET tracers for BCM analysis. The 77 corresponding ramatroban analogues containing a fluorine nuclide were characterized for properties including binding affinity, selectivity, and pharmacokinetic and metabolic profile, and 32 compounds with favorable properties were identified. This review illustrates the potential of GPR44 analogues for the development of PET tracers.
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http://dx.doi.org/10.3390/molecules26051433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961607PMC
March 2021

A new class of α-ketoamide derivatives with potent anticancer and anti-SARS-CoV-2 activities.

Eur J Med Chem 2021 Apr 10;215:113267. Epub 2021 Feb 10.

Nobel Institute of Biomedicine, Zhuhai, 519000, China; Ciechanover Institute of Precision and Regenerative Medicine, School of Life and Health Sciences, Chinese University of Hong Kong, Shenzhen, 518172, China. Electronic address:

Inhibitors of the proteasome have been extensively studied for their applications in the treatment of human diseases such as hematologic malignancies, autoimmune disorders, and viral infections. Many of the proteasome inhibitors reported in the literature target the non-primed site of proteasome's substrate binding pocket. In this study, we designed, synthesized and characterized a series of novel α-keto phenylamide derivatives aimed at both the primed and non-primed sites of the proteasome. In these derivatives, different substituted phenyl groups at the head group targeting the primed site were incorporated in order to investigate their structure-activity relationship and optimize the potency of α-keto phenylamides. In addition, the biological effects of modifications at the cap moiety, P1, P2 and P3 side chain positions were explored. Many derivatives displayed highly potent biological activities in proteasome inhibition and anticancer activity against a panel of six cancer cell lines, which were further rationalized by molecular modeling analyses. Furthermore, a representative α-ketoamide derivative was tested and found to be active in inhibiting the cellular infection of SARS-CoV-2 which causes the COVID-19 pandemic. These results demonstrate that this new class of α-ketoamide derivatives are potent anticancer agents and provide experimental evidence of the anti-SARS-CoV-2 effect by one of them, thus suggesting a possible new lead to develop antiviral therapeutics for COVID-19.
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http://dx.doi.org/10.1016/j.ejmech.2021.113267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873610PMC
April 2021

Strategies and progress in CXCR4-targeted anti-HIV therapeutic development.

Clin Infect Dis 2021 Feb 24. Epub 2021 Feb 24.

Center for Innovative Phage Applications and Therapeutics, Department of Medicine, Division of Infectious Diseases and Global Public Health, School of Medicine, University of California San Diego, La Jolla, U.S.A.

The acquired immunodeficiency syndrome (AIDS), caused by the human immunodeficiency virus (HIV), has been a global public health challenge for several decades. The majority of HIV infection is caused by the human immunodeficiency virus type 1 (HIV-1) which enters and infects a host cell via the cell surface proteins of CD4 as the primary receptor, and chemokine receptors CXCR4 or CCR5 as the co-receptor-then undergoing replication using the cell's intracellular machinery. Whereas many drugs targeting CCR5-mediated entry or HIV-1 replication via reverse transcriptase or proteases have long been used clinically, agents targeting CXCR4 are yet to be advanced to clinical application. Here in this review we highlight some of the strategies for and progress made in the discovery of novel small molecules, peptides, and larger molecules that target CXCR4, and their future prospects for translation into the clinic as a new class of anti-HIV therapeutics.
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http://dx.doi.org/10.1093/cid/ciab160DOI Listing
February 2021

Correction to: Glaciimonas soli sp. nov., a soil bacterium isolated from the forest of a high elevation mountain.

Antonie Van Leeuwenhoek 2021 Feb;114(2):223

Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, 10617, Taiwan.

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http://dx.doi.org/10.1007/s10482-020-01513-3DOI Listing
February 2021

Influence of Oxidation Damages on Mechanical Properties of SiC/SiC Composite Using Domestic Hi-Nicalon Type SiC Fibers.

Scanning 2020 8;2020:8840963. Epub 2020 Dec 8.

Key Laboratory of Advanced Functional Composites Technology, Aerospace Research Institute of Materials & Processing Technology, Beijing 100076, China.

Here, we show that when the oxidation treatment temperature exceeded 600°C, the tensile strength of SiC/SiC begins to decrease. Oxidation leads to the damages on the PyC fiber/matrix interface, which is replaced by SiO at higher temperature. The fracture mode converts from fiber pull-out to fiber-break as the fiber/matrix interface is filled with SiO. Oxidation time also plays an important role in affecting the tensile strength of SiC/SiC. The tensile modulus decreases with temperature from RT to 800°C, then increases above 800°C due to the decomposition of remaining CSi O and crystallization of the SiC matrix. A special surface densification treatment performed in this study is confirmed to be an effective approach to reduce the oxidation damages and improve the tensile strength of SiC/SiC after oxidation.
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http://dx.doi.org/10.1155/2020/8840963DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744235PMC
December 2020

Bifidobacteria in two-toed sloths (): phylogenetic characterization of the novel taxon sp. nov.

Int J Syst Evol Microbiol 2020 Dec;70(12):6115-6125

Department of Agricultural and Food Sciences, University of Bologna, Viale Fanin 44, 40127 Bologna, Italy.

Seven bifidobacterial strains were isolated from the faeces of two adult males of the two-toed sloth () housed in Parco Natura Viva, in Italy. Comparative sequence analysis of 16S rRNA and of five housekeeping (60, B, C, J, G) genes revealed that these strains were classified into two clusters. On the basis of 16S rRNA gene sequence similarity, the type strain of subsp. DSM 21854 (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6), whereas the type strain of DSM 20436 (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II. The average nucleotide identity (ANI) values of BRDM 6 and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively. Therefore, genotyping based on the genome sequence of the strain BRDM 6 combined with phenotypic analyses clearly revealed that the strain BRDM 6 represents a novel species for which the names sp. nov. (BRDM 6=NBRC 114053=BCRC 81222) is proposed.
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http://dx.doi.org/10.1099/ijsem.0.004506DOI Listing
December 2020

Development of a High-Resolution Single-Nucleotide Polymorphism Strain-Typing Assay Using Whole Genome-Based Analyses for the Probiotic Strain.

Microorganisms 2020 Sep 21;8(9). Epub 2020 Sep 21.

Bioresource Collection and Research Center, Food Industry Research and Development Institute, 331 Shih-Pin Rd, Hsinchu 30062, Taiwan.

is one of the most commonly used industrial products worldwide. Since its probiotic efficacy is strain-specific, the identification of probiotics at both the species and strain levels is necessary. However, neither phenotypic nor conventional genotypic methods have enabled the effective differentiation of strains. In this study, a whole-genome sequence-based analysis was carried out to establish high-resolution strain typing of 41 strains (including commercial isolates and reference strains) using the cano-wgMLST_BacCompare analytics platform; consequently, a strain-specific discrimination method for the probiotic strain LA1063 was developed. Using a core-genome multilocus sequence-typing (cgMLST) scheme based on 1390 highly conserved genes, 41 strains could be assigned to 34 sequence types. Subsequently, we screened a set of 92 loci with a discriminatory power equal to that of the 1390 loci cgMLST scheme. A strain-specific polymerase chain reaction combined with a multiplex minisequencing method was developed based on four (, , , and 1) out of 21 loci, which could be discriminated between LA1063 and other strains using the cgMLST data. We confirmed that the strain-specific single-nucleotide polymorphisms method could be used to quickly and accurately identify the probiotic strain LA1063 in commercial products.
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http://dx.doi.org/10.3390/microorganisms8091445DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564606PMC
September 2020

Phylogenetic characterization of two novel species of the genus Bifidobacterium: Bifidobacterium saimiriisciurei sp. nov. and Bifidobacterium platyrrhinorum sp. nov.

Syst Appl Microbiol 2020 Sep 9;43(5):126111. Epub 2020 Jul 9.

Department of Agricultural and Food Sciences, University of Bologna, Viale Fanin 44, 40127 Bologna, Italy.

Three bifidobacterial Gram-stain-positive, non-spore forming and fructose-6-phosphate phosphoketolase-positive strains, SMA1, SMB2 and SMA15 were isolated from the faeces of two adult males of the squirrel monkey (Saimiri sciureus). On the basis of 16S rRNA gene sequence similarities, the type strain of Bifidobacterium primatium DSM 100687 (99.3%; similarity) was the closest neighbour to strains SMA1 and SMB2, whereas the type strain of Bifidobacterium stellenboschense DSM 23968 (96.5%) was the closest neighbour to strain SMA15. The average nucleotide identity (ANI) values of SMA1 and SAM15 with the closely related type strains were 93.7% and 88.1%, respectively. The in silico DNA‒DNA hybridization values with the closest neighbours were 53.1% and 36.9%, respectively. GC contents of strains SMA1 and SMA15 were 63.6 and 66.4 mol%, respectively. Based on the phylogenetic, genotypic and phenotypic data obtained, the strains SMA1 and SMA15 clearly represent two novel taxa within the genus Bifidobacterium for which the names Bifidobacterium saimiriisciurei sp. nov. (type strain SMA1 = BCRC 81223 = NBRC 114049 = DSM 106020) and Bifidobacterium platyrrhinorum sp. nov. (type strain SMA15 = BCRC 81224 = NBRC 114051 = DSM 106029) are proposed.
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http://dx.doi.org/10.1016/j.syapm.2020.126111DOI Listing
September 2020

sp. nov., isolated from human faeces.

Int J Syst Evol Microbiol 2020 Aug;70(8):4767-4773

PRIME, Japan Agency for Medical Research and Development (AMED), Tsukuba, Ibaraki 305-0074, Japan.

A strictly anaerobic predominant bacterium, designated as strain gm001, was isolated from a freshly voided faecal sample collected from a healthy Taiwanese adult. Cells were Gram-stain-negative rods, non-motile and non-spore-forming. Strain gm001 was identified as a member of the genus , and a comparison of 16S rRNA and gene sequences revealed sequence similarities of 98.5 and 93.3 %, respectively, demonstrating that it was most closely related to the type strain of . Phylogenomic tree analysis indicated that the gm001 cluster is an independent lineage of DSM 18205. The average nucleotide identity, digital DNA‒DNA hybridization and average amino acid identity values between strain gm001 and DSM 18205 were 80.9, 28.6 and 83.8 %, respectively, which were clearly lower than the species delineation thresholds. The species-specific genes of this novel species were also identified on the basis of pan-genomic analysis. The predominant menaquinones were MK-11 and MK-12, and the predominant fatty acids were anteiso-C, C and iso-C. Acetate and succinate were produced from glucose as metabolic end products. Taken together, the results indicate that strain gm001 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is gm001 (=BCRC 81118=JCM 33280).
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http://dx.doi.org/10.1099/ijsem.0.004342DOI Listing
August 2020

Nutrition transition and related health challenges over decades in China.

Eur J Clin Nutr 2021 Feb 3;75(2):247-252. Epub 2020 Jul 3.

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, 100050, China.

Since the Opening of China, the country's economy has continuously and rapidly improved. Various economic, educational, and health policies have been implemented to shape the development of society, which may have greatly affected the Chinese diet and related malnutrition issues. The objective of the present review was to comprehensively review long-term trends in dietary intakes, nutrition status, and subsequent health challenges among Chinese adults. The data sources were mainly the 1982, 1992, 2002, and 2010-2012 China National Nutrition Surveys (CNNS) and reports and the 1989-2015 China Health and Nutrition Survey (CHNS). Over decades, there have been significant changes in the dietary structure of Chinese adults, characterized as decreased intake of cereals and vegetables and increased intake of animal foods with pork dominating. Intakes of eggs, fish, and dairy has reminded at a low level, with only a small increase over time. Consumption of cooking oil and salt was substantively far above the recommendations. A great proportion of fat-to-energy intake and "hidden hunger" was still prominent. Despite nutrition deficiency, there have been some modest improvements in related diseases, but overweight and obesity has become a prominent issue, with the prevalence in adults increasing from 16.4% and 3.6% in 1982 to 30.1% and 11.9% in 2012, respectively. In conclusion, this review sheds light on some salient problems with nutrition and malnutrition status in China, especially the dual challenges of undernutrition and overnutrition. Dynamic monitoring of nutritional characteristics in China should be strengthened, and effective strategies to improve nutrition need to be targeted at the national, societal, family, and individual levels.
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http://dx.doi.org/10.1038/s41430-020-0674-8DOI Listing
February 2021

Clinical Efficacy of Combined Hysteroscopic and Laparoscopic Surgery and Reversible Ligation of the Uterine Artery for Excision and Repair of Uterine Scar in Patients with Type II and III Cesarean Scar Pregnancy.

Med Sci Monit 2020 Jun 29;26:e924076. Epub 2020 Jun 29.

Department of Obstetrics and Gynecology, Ningbo Women's and Children's Hospital, Ningbo, Zhejiang, China (mainland).

BACKGROUND With the changes in China's family planning policy, the incidence of cesarean scar pregnancy (CSP) significantly increased in recent years. The present study aimed to investigate the clinical efficacy of combined hysteroscopic and laparoscopic surgery and reversible ligation of the uterine artery for cesarean scar excision and repair in patients with type II and III CSP. MATERIAL AND METHODS This was a retrospective study of 173 patients with type II and III CSP. They were assigned to the hysteroscopy and laparoscopy group (group A), hysteroscopy group (group B), and curettage group (group C) according to the surgery they underwent. The surgical indicators (intraoperative bleeding volume and hospital stay), postoperative recovery (time of serum ß-hCG returning to the normal, postoperative residual lesion, the thickness of the uterine scar, and recovery time of menstruation), and the postoperative complications were compared among the 3 groups. RESULTS In patients with type II and III CSP, significant differences (P<0.05) were observed between group A vs. groups B and C in terms of the time of serum ß-HCG returning to normal, postoperative residual lesions, the thickness of the uterine scar, and recovery time of menstruation, while there were no significant differences in intraoperative bleeding volume and postoperative hospital stay (P>0.05). CONCLUSIONS For patients with type II and III CSP, hysteroscopy and laparoscopy surgery and reversible ligation of the uterine artery achieved better clinical outcomes than hysteroscopy or curettage with respect to postoperative recovery. This could be suitable for patients with CSP and desire for fertility.
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http://dx.doi.org/10.12659/MSM.924076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7341695PMC
June 2020

Application of an education model using the WeChat public platform in the standardized training of anesthesiology residents.

Ann Palliat Med 2020 Jul 15;9(4):1643-1647. Epub 2020 Jun 15.

Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Standardized training of clinical residents is an important way to train high-level clinicians and is an important step to ensure quality clinical work. WeChat facilitates efficient information dissemination and feedback, convenient communication. In the new media era, the influence of WeChat has inspired changes in educational methods, to explore the role of an education model using the WeChat public platform in the standardized training of anesthesiology residents.

Methods: A total of 40 anesthesiology residents undergoing standardized training in our department from January 2016 to August 2017 were selected as subjects and randomly divided into 2 groups: traditional group (n=20) and WeChat group (n=20). In the WeChat group, in addition to the traditional clinical teaching model (lectures), the residents also received push information from the WeChat public platform for anesthesia education. The residents in the traditional group did not receive the push information and only received traditional clinical teaching. Three months later, assessment scores of and teaching quality in the 2 groups were evaluated. Teaching quality was evaluated using peer assessment and self-assessment questionnaires.

Results: Residents in the WeChat group performed significantly better on assessments than those in traditional group regarding theoretical knowledge of anesthesiology, understanding mechanisms, operation ability, current knowledge, case analyses and use of professional English (P<0.05). The questionnaire results indicated that the degree of satisfaction of the residents and teachers in the WeChat group was significantly higher than that in the traditional group (P<0.05). The theoretical knowledge scores, operational skill scores and overall scores in the WeChat group were significantly higher than those in the traditional group (P<0.05).

Conclusions: Applying an education model based on the WeChat public platform in standardized training of anesthesiology residents can significantly improve teaching efficacy and satisfaction, enhance comprehensive assessment results, and improve teaching quality.
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http://dx.doi.org/10.21037/apm-19-390DOI Listing
July 2020

Design, synthesis, and biological characterization of a new class of symmetrical polyamine-based small molecule CXCR4 antagonists.

Eur J Med Chem 2020 Aug 16;200:112410. Epub 2020 May 16.

Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China. Electronic address:

CXCR4, a well-studied coreceptor of human immunodeficiency virus type 1 (HIV-1) entry, recognizes its cognate ligand SDF-1α (also named CXCL12) which plays many important roles, including regulating immune cells, controlling hematopoietic stem cells, and directing cancer cells migration. These pleiotropic roles make CXCR4 an attractive target to mitigate human disorders. Here a new class of symmetrical polyamines was designed and synthesized as potential small molecule CXCR4 antagonists. Among them, a representative compound 21 (namely HF50731) showed strong CXCR4 binding affinity (mean IC = 19.8 nM) in the CXCR4 competitive binding assay. Furthermore, compound 21 significantly inhibited SDF-1α-induced calcium mobilization and cell migration, and blocked HIV-1 infection via antagonizing CXCR4 coreceptor function. The structure-activity relationship analysis, site-directed mutagenesis, and molecular docking were conducted to further elucidate the binding mode of compound 21, suggesting that compound 21 could primarily occupy the minor subpocket of CXCR4 and partially bind in the major subpocket by interacting with residues W94, D97, D171, and E288. Our studies provide not only new insights for the fragment-based design of small molecule CXCR4 antagonists for clinical applications, but also a new and effective molecular probe for CXCR4-targeting biological studies.
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http://dx.doi.org/10.1016/j.ejmech.2020.112410DOI Listing
August 2020

Glaciimonas soli sp. nov., a soil bacterium isolated from the forest of a high elevation mountain.

Antonie Van Leeuwenhoek 2020 Aug 28;113(8):1213-1223. Epub 2020 May 28.

Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, 10617, Taiwan.

A Gram-negative, psychrophilic bacterium, designated strain GS1, was isolated from a forest soil sample collected from the West Peak of Mt. Yushan, Yushan National Park, Taiwan. Cells grown in broth cultures were mostly non-motile and non-flagellated, whereas motile cells with monotrichous, subpolar flagella were also observed. The novel strain grew over a temperature range of 4-25 °C with optimum growth at 10-15 °C. It grew aerobically and was not capable of anaerobic growth by fermentation of D-glucose or other carbohydrates. Ubiquinone 8 was the predominant isoprenoid quinone. The major polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol and dimethylaminoethanol. Cellular fatty acids were dominated by Cω7c (35.2%), C (19.5%), Cω7c (18.8%) and Cω7c cyclo (15.5%). The DNA G + C content was 49.2 mol% evaluated according to the genomic sequencing data. Strain GS1 shared more than 96.5% 16S rRNA gene sequence similarities with type strains of four Collimonas species (97.2-97.5%), three Glaciimonas species (97.3% for each of the three) and Oxalicibacterium solurbis (96.5%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GS1 formed a stable genus-level clade with type strains of species in the genus Glaciimonas in the family Oxalobacteraceae and GS1 was an outgroup with respect to these Glaciimonas species. Characteristically, strain GS1 could be easily distinguished from the recognised Glaciimonas species by exhibition of swimming motility with monotrichous, subpolar flagellum in some of the cells, ability to grow in NaCl at 2% but not at 3% and the distinguishable fatty acid profiles. On the basis of the polyphasic taxonomic data from this study, strain GS1 is considered to represent a novel species of the genus Glaciimonas, for which the name Glaciimonas soli sp. nov. is proposed. The type strain is GS1 (= JCM 33275 = BCRC 81091).
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http://dx.doi.org/10.1007/s10482-020-01428-zDOI Listing
August 2020

Genome-based reclassification of : emended classification and description of the species .

Int J Syst Evol Microbiol 2020 Jun;70(6):3755-3762

Bioresource Collection and Research Center, Food Industry Research and Development Institute, 331 Shih-Pin Rd, Hsinchu 30062, Taiwan, ROC.

Taxonomic relationships between , and have long been debated. Results of previous analyses have shown that overall genome relatedness indices (such as average nucleotide identity and core nucleotide identity) between the type strains ATCC 393 and ATCC 15820 were 94.6 and 95.3 %, respectively, which are borderline for species definition. However, the digital DNA‒DNA hybridization value was 57.3 %, which was clearly lower than the species delineation threshold of 70 %, and hence raised the possibility that could be reclassified into two species. To re-evaluate the taxonomic relationship of these taxa, multilocus sequence analysis (MLSA) based on the concatenated five housekeeping gene (, , , and ) sequences, phylogenomic and core genome multilocus sequence typing analyses, gene presence and absence profiles using pan-genome analysis, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling analysis, cellular fatty acid compositions, and phenotype analysis were carried out. The results of phenotypic characterization, MLSA, whole-genome sequence-based analyses and MALDI-TOF MS profiling justified an independent species designation for the strains, and supported an emended the description of the name of (ex Kuznetsov 1956) Dicks . 1996, with ATCC 15820 (=DSM 20178=BCRC 17942) as the type strain.
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http://dx.doi.org/10.1099/ijsem.0.003969DOI Listing
June 2020

Experimental and Theoretical Study on the False Positive of Monomethyltin Determination in Toys Based on Gastric Juice Migration.

Int J Anal Chem 2020 31;2020:1975471. Epub 2020 Mar 31.

School of Chemistry and Chemical Engineering, South China University of Technology, Wushan Road 381, Tianhe District, Guangzhou 510640, China.

Background: Organic tin compounds (OTCs), a group of high-risk hazardous substances, are highly concerned in safety regulation of consumer products especially for toys because they can cause serious damage to organs after prolonged or repeated exposure. Gastric juice migration is nowadays widely implemented to assess organic tin intake from toys or food-contact materials; however, the followed up detection method using sodium tetraethylborate [NaB(Et)] as a derivatization agent may directly lead to false positive of monomethyltins (MMT).

Objective: In order to avoid the phenomena of false positive of MMT in the course of laboratory testing of toys, it is necessary and important to perform relative experimental and theoretical studies to reveal the cause of false positive of MMT.

Method: With metal tin powder as a representative of inorganic tin which existed in real samples, it was treated with artificial gastric juice (0.07 mol/L·HCl), followed by ethyl derivatization using sodium tetraethylborate [NaB(Et)] and then analyzed by gas chromatography-mass spectrometry (GC-MS) according to the procedure specified in the standard of EN 71-3:2013+A3:2018 issued by the European Committee for Standardization (CEN).

Results: Without any OTCs in the starting materials, MMT false positive can be reproduced by detecting 0.56 mg/L of triethylmethyltin (TEMT) together with approximately 1000 mg/L of tetraethyltin (TeET), which is similar to real samples. Further, it is demonstrated that the detected amount of TEMT is linearly related with the added amount of NaB(Et), and that the formation of TEMT (methyl derivative) is easier than TeET (ethyl derivative) even though the ethyl group is present in a larger amount than the methyl group.

Conclusions: The phenomena of MMT false positive which occurred in the laboratory testing of toys is mainly because that TEMT is highly likely to be obtained from the reaction of inorganic tin and trace level of methylation agent impurities contained in the derivatization reagent-NaB(Et). To avoid MMT false positive, it is concluded that the maximum acceptable mole ratio of methylation agent impurities contained in NaB(Et) is approximately 0.028%. This research is helpful to be aware of methylation impurities and is favorable to avoid false judgment caused by MMT false positive in routine analysis of toys.
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http://dx.doi.org/10.1155/2020/1975471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150698PMC
March 2020

sp. nov., isolated from faeces of Formosan barking deer ().

Int J Syst Evol Microbiol 2020 Mar;70(3):1578-1584

Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute, 331 Shih-Pin Rd., Hsinchu 30062, Taiwan, ROC.

A Gram-stain-positive strain, 8 H-2, was isolated from faeces of Reeves' muntjac () barking deer in Taiwan. Cells of the strain were short rod-shaped, non-motile, non-haemolytic, asporogenous, facultatively anaerobic, heterofermentative and did not exhibit catalase and oxidase activities. Comparative analyses of 16S rRNA, and gene sequences demonstrated that the novel strain was a member of the genus . On the basis of 16S rRNA gene sequence similarities, the type strains of (99.2 %), (97.8 %), (97.6 %) and (97.3 %) were the closest neighbours to strain 8 H-2. The concatenated housekeeping gene sequence ( and ) similarities of 8 H-2 to closely related type strains were 72.5-84.9 %, respectively. The genomic DNA G+C content was 40.5 mol%. The average nucleotide identity and digital DNA-DNA hybridization values with these type strains were 70.2-75.4% and 25.1-30.1 %, respectively. Phenotypic and genotypic test results demonstrated that strain 8 H-2 represents a novel species belonging to the genus , for which the name sp. nov. is proposed. The type strain is 8 H-2 (=BCRC 81133=NBRC 113537).
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http://dx.doi.org/10.1099/ijsem.0.003937DOI Listing
March 2020

Natural killer cells in the pathogenesis of preeclampsia: a double-edged sword.

J Matern Fetal Neonatal Med 2020 Mar 19:1-8. Epub 2020 Mar 19.

Department of Obstetrics and Gynecology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong, China.

To investigate the relationship between natural killer (NK) cells, extravillous trophoblast cells (EVTs) and vessel remodeling in early human pregnancy, and the association between NK cells and preeclampsia (PE) in late human pregnancy. Human decidual tissues from women with normal pregnancies were collected and examined for the relationship of NK cells with uterine vessel remodeling using immunohistochemistry. Percentages of peripheral blood NK (pNK) and decidual NK (dNK) cells and the levels of intracellular interferon (IFN)-γ, perforin and granzyme B in normal pregnancies, late-onset and early-onset PE were analyzed using flow cytometry. Cytolytic functions of dNK cells from normal and PE pregnancies were examined. Effects of conditioned medium (CM) of dNK cells from normal and PE pregnancies on first trimester trophoblast invasion and migration were tested. In early pregnancy samples (9-13 weeks of gestation), we noted moderate vessel remodeling with abundant perivascular NK cells but a limited number of surrounding EVTs. The numbers of both human pNK cells and dNK cells and intracellular interferon (IFN)-γ, perforin and granzyme B production were significantly higher in PE compared with normal pregnancies at the time of delivery for both early- and late-onset disease. dNK cells from PE pregnancies not only killed first trimester trophoblasts but also inhibited their invasion and migration when compared to normal controls. Our results suggest that NK cells, in conjunction with EVTs, may play an important role in controlling uterine SA remodeling at the early stages of vessel remodeling, but they contribute to the pathogenesis of PE in late pregnancy.
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http://dx.doi.org/10.1080/14767058.2020.1740675DOI Listing
March 2020

Associations of Dietary Sodium, Potassium, and Sodium to Potassium Ratio with Blood Pressure- Regional Disparities in China.

Nutrients 2020 Jan 30;12(2). Epub 2020 Jan 30.

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

High dietary sodium and low potassium intake increase blood pressure and risk of hypertension, but whether the relationship between dietary sodium and potassium and risk of hypertension is different in North China and South China remains unclear. We used data from the longitudinal China Health and Nutrition Survey (CHNS) and selected 6705 adults who participated in at least two waves in 2009, 2011, and 2015 and had no hypertension in baseline. We performed multiple linear regression analysis and multiple logistic regressions stratified by area for the present study design. Sodium and potassium intake were higher in North China (4343.4 and 1624.8 mg/day, respectively) than in South China (4107.8 and 1516.1 mg/d, respectively) ( < 0.05). Multiple linear regression revealed that a positive correlation of sodium intake (β = 0.026, < 0.05) and ratio of sodium to potassium (Na-K) intake (β = 0.041, < 0.01) with diastolic blood pressure (DBP) was found in North China, and the association of sodium, potassium, and Na-K intake ratio with blood pressure was different in South China. Multiple logistic regressions documented a similar significant inverse association between dietary potassium intake and risk of hypertension in both North China and South China (risk ratio (RR): 0.63, 95%CI: 0.50-0.79; RR: 0.80, 95%CI: 0.66-0.98, respectively). The risk of hypertension increased in the fourth quartile of dietary sodium and Na-K intake ratio (RR: 1.20, 95%CI: 1.00-1.44; RR: 1.35, 95%CI: 1.13-1.62, respectively) in North China but no association was observed in South China. The current study indicates a different association of dietary sodium and Na-K intake ratio with systolic blood pressure (SBP), DBP, and risk of hypertension in North China and South China.
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http://dx.doi.org/10.3390/nu12020366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071283PMC
January 2020

sp. nov., isolated from brewer's grain used to make silage in Taiwan.

Int J Syst Evol Microbiol 2020 Mar;70(3):1953-1960

Department of Animal Science and Technology, College of Bioresources and Agriculture, National Taiwan University, No. 50, Ln. 155, Sec. 3, Keelung Rd., Taipei 10673, Taiwan, ROC.

A Gram-stain-positive, coccus- or oval-shaped, non-motile, haemolytic, asporogenous, catalase- and oxidase-negative, and facultatively anaerobic strain, 2B-2, was isolated from a brewer's grain used to make silage in Taiwan. Comparative analyses of 16S rRNA, and gene sequences demonstrated that strain 2B-2 was a member of the genus . On the basis of 16S rRNA gene sequence similarity, the type strains of (98.4 % similarity), (98.4 %), (98.2 %), (98.2 %) and (98.0 %) were the closest neighbours to this novel strain. The similarity levels of concatenated housekeeping gene sequences ( and ) between strain 2B-2 and these closely related species ranged from 84.5 to 88.0 %. The average nucleotide identity and DNA-DNA hybridization values between strain 2B-2 and its closest relatives were lower than 72.9 and 21.6 %, respectively. The DNA G+C content was 34.7 mol%. Phenotypic and genotypic features demonstrated that strain 2B-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 2B-2 (=BCRC 81132=NBRC 113536).
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http://dx.doi.org/10.1099/ijsem.0.003999DOI Listing
March 2020

Phillyrin Relieves Lipopolysaccharide-Induced AKI by Protecting Against Glycocalyx Damage and Inhibiting Inflammatory Responses.

Inflammation 2020 Apr;43(2):540-551

Department of Cell Biology, Binzhou Medical University, Yantai, China.

Damage to the integrity of heparin sulfate (HS) in the endothelial glycocalyx is an important factor of glomerular filtration barrier dysfunction, which is the basic pathological feature of acute kidney injury (AKI). AKI is a common clinical critical illness with few drugs options offering effective treatment. Phillyrin (Phil), the main pharmacological component of Forsythia suspensa, possesses a wide range of pharmacological activities. However, the effects of Phil on lipopolysaccharide (LPS)-induced AKI have yet to be reported. The aim of the present study is to analyze the effects of Phil on HS damage and inflammatory signaling pathways in LPS-induced AKI. Results revealed that Phil reduces pathological changes and improves renal function in LPS-induced AKI. Further analysis indicated that Phil effectively protects against glycocalyx HS degradation in LPS-stimulated EA.hy926 cells in vitro and LPS-induced AKI mice in vivo. The protective effect of Phil on HS damage may be associated with the isolate's ability to suppress the production of reactive oxygen species, and decrease expression levels of cathepsin L and heparanase in vitro and in vivo. In addition, ELISA and Western blot results revealed that Phil inhibits the activation of the NF-κB and MAPK signaling pathways and decreases the levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) in LPS-induced ARDS mice. In general, protection against endothelial glycocalyx HS damage and inhibition of inflammatory responses by Phil may be used as treatment targets for LPS-induced AKI.
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http://dx.doi.org/10.1007/s10753-019-01136-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7095384PMC
April 2020

Challenges and future perspectives on microwave absorption based on two-dimensional materials and structures.

Nanotechnology 2020 Apr 23;31(16):162001. Epub 2019 Oct 23.

Shenzhen Key Laboratory of Laser Engineering, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, People's Republic of China.

The widespread use of electronic equipment, such as computers, cell phones, communication devices and wireless facilities, has increased electromagnetic radiation, which can cause cancer and other diseases in humans. Furthermore, there is an urgent need for excluding the interferences in the aircraft and other precise instruments in military aspects. Therefore, minimizing and attenuating electromagnetic waves are critical issues. In this review, various two-dimensional (2D) materials and structures are discussed for microwave-absorbing and shielding in terms of 'thin, light, wide, and strong' requirements. The typical absorption and attenuation mechanisms are analysed and summarized to deliver an overall view and offer possible trends for future developments. Multiple works have revealed that 2D materials and structures are promising for use in microwave devices. In addition to conventional materials with 2D structures, we focus on new graphene-like materials, such as 2D transition metal dichalcogenides and black phosphorus, due to their beneficial absorbing and shielding properties. These 2D materials will likely play an important role in electromagnetic wave absorption and cancellation in the future. Finally, the related challenges and some new 2D materials are briefly discussed.
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http://dx.doi.org/10.1088/1361-6528/ab50afDOI Listing
April 2020

Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp. nov. and B. rousetti sp. nov.

Syst Appl Microbiol 2019 Nov 23;42(6):126017. Epub 2019 Sep 23.

Department of Agricultural Sciences, University of Bologna, Viale Fanin 44, 40127 Bologna, Italy. Electronic address:

Fifteen bifidobacterial strains were obtained from faeces of Rousettus aegyptiacus; after grouping them by RAPD PCR only eight were selected and characterized. Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16), Cluster II (RST 9 and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685 (96.3%), Bifidobacterium callitrichos DSM 23973 (99.2% and 99.7%), Bifidobacterium tissieri DSM 100201 (99.7 and 99.2%), Bifidobacterium reuteri DSM 23975 (98.9%) and Bifidobacterium myosotis DSM 100196 (99.3%), respectively. Strains in Cluster I and strain RST 9 in Cluster II could not be placed within any recognized species while the other ones were identified as known species. The average nucleotide identity values between two novel strains, RST 16 and RST 9 and their closest relatives were lower than 79% and 89%, respectively. In silico DNA-DNA hybridization values for those closest relatives were 32.5 and 42.1%, respectively. Phenotypic and genotypic tests demonstrated that strains in Cluster I and RST 9 in Cluster II represent two novel species for which the names Bifidobacterium vespertilionis sp. nov. (RST 16=BCRC 81138=NBRC 113380=DSM 106025 ; RST 8=BCRC 81135=NBRC 113377) and Bifidobacterium rousetti sp. nov. (RST 9=BCRC 81136=NBRC 113378=DSM 106027) are proposed.
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http://dx.doi.org/10.1016/j.syapm.2019.126017DOI Listing
November 2019

Evaluating the biochemical changes of LPS-stimulated endothelial cells by synchrotron FTIR microspectroscopy at a single-cell level.

Anal Bioanal Chem 2019 Nov 6;411(27):7157-7164. Epub 2019 Sep 6.

Zhangjiang Lab, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, China.

Endothelial damage is a major manifestation in many forms of heart and lung injuries induced by lipopolysaccharide (LPS), but the biochemical responses and activation mechanisms of endothelial cells have not been fully explicit. In this study, the biochemical changes to endothelial cells exposed to LPS were investigated by synchrotron FTIR microspectroscopy at a single-cell level. We found that the whole infrared spectrum of endothelial cells shifted after LPS treatment, indicating chemical component changes within cells. Principal component analysis (PCA) and t tests on subspectra (fatty acid region, protein region, and nucleic acid-sugar region, respectively) further showed that sugar components as well as fatty acids changed dramatically while proteins had no significant variation following LPS exposure. These results suggested that the glycocalyx layer structure on endothelial cell membrane may be mainly influenced by LPS and also proved that synchrotron FTIR microspectroscopy was a useful technique to evaluate the biochemical changes of endothelial damage at the single-cell level. Graphical abstract.
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http://dx.doi.org/10.1007/s00216-019-02083-8DOI Listing
November 2019

Reclassification of and as later heterotypic synonyms of .

Int J Syst Evol Microbiol 2019 Nov;69(11):3512-3518

Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute, 331 Shih-Pin Rd., Hsinchu 30062, Taiwan, ROC.

, and are phenotypically and genotypically closely related, and together comprise the group. In this study, the taxonomic relationships among , and were re-evaluated by using polyphasic approaches. The similarity values of the concatenated housekeeping gene (, and ) sequences shared by the type strains of , and ranged from 98.3 to 99.4 %. The average nucleotide identity, average amino acid identity and digital DNA‒DNA hybridization values among these three taxa were greater (97.1‒98.1 %, 96.8‒98.1 % and 75.0‒83.5 %, respectively) than the thresholds for bacterial species delineation, indicating that they belong to the same species, whereas those for were clearly lower than the thresholds. In addition, phenotypic and chemotaxonomic characterization results also support the synonymy of these three taxa. Therefore, we propose that and should be reclassified as later heterotypic synonyms of .
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http://dx.doi.org/10.1099/ijsem.0.003654DOI Listing
November 2019

Draft Genome Sequence of sp. nov. Strain gm002, Isolated from Human Feces.

Microbiol Resour Announc 2019 Aug 1;8(31). Epub 2019 Aug 1.

Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan, Republic of China.

Here, we report a draft genome sequence of sp. strain gm002, isolated from human feces in Taiwan. This strain represents a novel species according to whole-genome-based comparisons using the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) thresholds. The genome assembly comprised 3,568,768 bp, with a 38.7% G+C content.
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http://dx.doi.org/10.1128/MRA.00217-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6675978PMC
August 2019