Publications by authors named "Lin Zhu"

1,859 Publications

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Defibrotide suppresses brain metastasis by activating the adenosine A2A receptors.

Anticancer Drugs 2022 Aug 10. Epub 2022 Aug 10.

Hebei General Hospital, No. 348 Heping West Road, Shijiazhuang, Hebei, China.

Brain metastasis is a devastating clinical condition globally as one of the most common central nervous system malignancies. The current study aimed to assess the effect of defibrotide, an Food and Drug Administration-approved drug, against brain metastasis and the underlying molecular mechanisms. Two tumor cell lines with high brain metastasis potential, PC-9 and 231-BR, were subjected to defibrotide treatment of increasing dosage. The metastasis capacity of the tumor cells was evaluated by cell invasion and migration assays. Western blotting was employed to determine the levels of tight junction proteins in the blood-brain barrier (BBB) including Occludin, Zo-1, and Claudin-5, as well as metastasis-related proteins including CXCR4, MMP-2, and MMP-9. The in-vitro observations were further verified in nude mice, by monitoring the growth of xenograft tumors, mouse survival and brain metastasis foci following defibrotide treatment. Defibrotide inhibited proliferation, migration, invasion, and promotes lactate dehydrogenase release of brain metastatic tumor cells, elevated the levels of BBB tight junction proteins and metastasis-related proteins. Such beneficial role of defibrotide was mediated by its inhibitory action on the SDF-1/CXCR4 signaling axis both in vitro and in vivo, as CXCR4 agonist SDF1α negated the anti-tumoral effect of defibrotide on mouse xenograft tumor growth, mouse survival and brain metastasis. Defibrotide inhibits brain metastasis through activating the adenosine A2A receptors, which in turn inhibits the SDF-1/CXCR4 signaling axis. Our study hereby proposes defibrotide as a new and promising candidate drug against brain metastasis of multiple organ origins.
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http://dx.doi.org/10.1097/CAD.0000000000001372DOI Listing
August 2022

Effective and stable adsorptive removal of Cadmium(II) and Lead(II) using selenium nanoparticles modified by microbial SmtA metallothionein.

Chemosphere 2022 Aug 6:135818. Epub 2022 Aug 6.

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China. Electronic address:

Metallothionein SmtA-modified selenium nanoparticles (SmtA-SeNPs), efficient adsorbents for Cd(II) and Pb(II), were synthesized in the present work. The ligand, microbial SmtA protein, was synthesized using an engineered strain Escherichia coli, posing the benefits of simplicity, safety, and high production. SmtA-SeNPs were spheres with diameters between 68.1 and 122.4 nm, containing amino, hydroxyl, and sulfhydryl functional groups with negatively charged (pH > 5). SmtA-SeNPs displayed better adsorption performance than dissociative SmtA and SeNPs. The adsorption of Cd(II) and Pb(II) mainly depends on the electrostatic attractions and the metal chelation of abundant functional groups. The maximum adsorption capacity was 506.3 mg/g for Cd(II) and 346.7 mg/g for Pb(II), which were higher than the values of most nanoparticles. In addition, SmtA-SeNPs were immobilized with a membrane filter to produce a SmtA-SeNPs filter, and the percentage removal of Cd(II) and Pb(II) increased from 26.75% to 98.13% for Cd(II) and from 9.95% to 99.20% compared with the blank filter. Moreover, the SmtA-SeNPs filter was regenerated using subacid deionized water, and the filter exhibited a stable removal ratio of Cd(II) and Pb(II) in ten continuous cycles of Cd(II)- or Pb(II)-containing wastewater treatment. The residual amounts of Cd and Pb met national standard levels of wastewater discharge.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135818DOI Listing
August 2022

Novel Polarity Fluorescent Probe for Dual-Color Visualization of Lysosomes and Plasma Membrane during Apoptosis.

Anal Chem 2022 Aug 9. Epub 2022 Aug 9.

Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, P. R. China.

Apoptosis plays a crucial role in the occurrence of cancer and other diseases. Real-time monitoring of the cell apoptosis process has great significance for cell viability and drug screening. Herein, a novel fluorescent probe was constructed based on the fluorescence resonance energy transfer mechanism, which track the sensitivity of polarity changes, as well as detect the drug-induced cell apoptosis process in a dual-color mode. Importantly, the change of cellular microenvironmental polarity makes it possible to dynamically visualize the process of drug-induced cell apoptosis. More significantly, the designed probe targeted the lysosomes in the living cells to give a blue emission, and it accumulated on the plasma membrane to display red fluorescence during the drug-induced cell apoptosis process. Thus, cell viability could be monitored by both the localization and emission colors of the robust probe. We expect that the unique probe can provide a new blueprint for evaluating and screening apoptosis-related drugs.
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http://dx.doi.org/10.1021/acs.analchem.2c02207DOI Listing
August 2022

Regulation of TFEB nuclear localization by HSP90AA1 promotes autophagy and longevity.

Autophagy 2022 Aug 8:1-17. Epub 2022 Aug 8.

Department of Experimental Surgery, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi, China.

TFEB (transcription factor EB) regulates multiple genes involved in the process of macroautophagy/autophagy and plays a critical role in lifespan determination. However, the detailed mechanisms that regulate TFEB activity are not fully clear. In this study, we identified a role for HSP90AA1 in modulating TFEB. HSP90AA1 was phosphorylated by CDK5 at Ser 595 under basal condition. This phosphorylation inhibited HSP90AA1, disrupted its binding to TFEB, and impeded TFEB's nuclear localization and subsequent autophagy induction. Pro-autophagy signaling attenuated CDK5 activity and enhanced TFEB function in an HSP90AA1-dependent manner. Inhibition of HSP90AA1 function or decrease in its expression significantly attenuated TFEB's nuclear localization and transcriptional function following autophagy induction. HSP90AA1-mediated regulation of a TFEB ortholog was involved in the extended lifespan of in the absence of its food source bacteria. Collectively, these findings reveal that this regulatory process plays an important role in modulation of TFEB, autophagy, and longevity. : AL: autolysosome; AP: autophagosome; ATG: autophagy related; BafA1: bafilomycin A; CDK5: cyclin-dependent kinase 5; CDK5R1: cyclin dependent kinase 5 regulatory subunit 1; CR: calorie restriction; FUDR: 5-fluorodeoxyuridine; HSP90AA1: heat shock protein 90 alpha family class A member 1; MAP1LC3: microtubule associated protein 1 light chain 3; NB: novobiocin sodium; SQSTM1: sequestosome 1; TFEB: transcription factor EB; WT: wild type.
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http://dx.doi.org/10.1080/15548627.2022.2105561DOI Listing
August 2022

An Individualized Recommendation for Controlled Ovary Stimulation Protocol in Women Who Received the GnRH Agonist Long-Acting Protocol or the GnRH Antagonist Protocol: A Retrospective Cohort Study.

Front Endocrinol (Lausanne) 2022 22;13:899000. Epub 2022 Jul 22.

Department of Obstetrics and Gynecology, Women and Children's Hospital of Chongqing Medical University, Chongqing, China.

Background: The GnRH agonist long-acting protocol and GnRH antagonist protocol are widely used in ovarian stimulation. Which protocol eliciting higher live birth rate for IVF/ICSI patients with different ages, different ovarian reserves and different body mass index (BMI) has not been studied. However, among these protocols, the one that elicits higher live birth in IVF/ICSI patients with different ages, ovarian reserves and body mass indexes (BMI) has not been identified.

Methods: This was a retrospective cohort study about 8579 women who underwent the first IVF-ET from January, 2018 to August, 2021. Propensity Score Matching (PSM) was used to improve the comparability between two protocols.

Results: After PSM, significant higher live birth rates were found in the GnRH agonist long-acting protocol compared to GnRH antagonist protocol (44.04% vs. 38.32%) (p<0.001). Stratified analysis showed that for those with AMH levels between 3 ng/ml and 6 ng/ml, with BMI ≥ 24 kg/m and were aged ≥ 30 years old, and for those women with BMI < 24kg/m and were aged ≥30 years whose AMH levels were ≤ 3ng/ml, the GnRH agonist long-acting protocol was more likely to elicit live births [OR (95%CI), 2.13(1.19,3.80)], [OR (95%CI), 1.41(1.05,1.91)]. However, among women with BMI ≥ 24kg/m and were aged ≥30 years whose AMH levels were ≤ 3ng/ml, the GnRH agonist long-acting protocol had a lower possibility of eliciting live births [OR (95%CI), 0.54(0.32,0.90)]. Also, among women with AMH levels between 3 ng/ml and 6 ng/ml, with BMI ≥ 24 kg/m and with age < 30 years and for those with AMH levels between 3 ng/ml and 6 ng/ml, regardless of age, and with BMI<24kg/m2,, the possibility of live births was similar between the two protocols [OR (95%CI), 1.06(0.60,1.89)], [OR (95%CI), 1.38(0.97,1.97)], [OR (95%CI), 0.99(0.72,1.37)]. Among the women with AMH levels ≤ 3 ng/ml and with were aged < 30years, regardless of BMI, the possibility of live birth was similar between the two protocols [OR (95%CI), 1.02(0.68,1.54)], [OR (95%CI), 1.43(0.68,2.98)]. Moreover, among women with AMH levels ≥ 6ng/ml, the possibility of live birth was similar between the two protocols [OR (95%CI),1.42(0.75,2.69)], [OR (95%CI),1.02(0.19,5.35)], [OR (95%CI), 1.68(0.81,3.51)], [OR (95%CI), 0.51(0.10,2.55)].

Conclusions: The suitability of the GnRH agonist long-acting protocol or GnRH antagonist protocol to infertility patients is dependent on specific biological characteristics of the patients.
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http://dx.doi.org/10.3389/fendo.2022.899000DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355571PMC
August 2022

High-frequency repetitive transcranial magnetic stimulation (rTMS) protects against ischemic stroke by inhibiting M1 microglia polarization through let-7b-5p/HMGA2/NF-κB signaling pathway.

BMC Neurosci 2022 08 4;23(1):49. Epub 2022 Aug 4.

Department of Neurology, Nanjing First Hospital, Nanjing Medical University, 68# Changle Road, Nanjing, 210029, Jiangsu, China.

Background: Microglia assume opposite phenotypes in response to ischemic brain injury, exerting neurotoxic and neuroprotective effects under different ischemic stages. Modulating M1/M2 polarization is a potential therapy for treating ischemic stroke. Repetitive transcranial magnetic stimulation (rTMS) held the capacity to regulate neuroinflammation and astrocytic polarization, but little is known about rTMS effects on microglia. Therefore, the present study aimed to examine the rTMS influence on microglia polarization and the underlying possible molecular mechanisms in ischemic stroke models.

Methods: Previously reported 10 Hz rTMS protocol that regulated astrocytic polarization was used to stimulate transient middle cerebral artery occlusion (MCAO) rats and oxygen and glucose deprivation/reoxygenation (OGD/R) injured BV2 cells. Specific expression levels of M1 marker iNOS and M2 marker CD206 were measured by western blotting and immunofluorescence. MicroRNA expression changes detected by high-throughput second-generation sequencing were validated by RT-PCR and fluorescence in situ hybridization (FISH) analysis. Dual-luciferase report assay and miRNA knock-down were applied to verify the possible mechanisms regulated by rTMS. Microglia culture medium (MCM) from different groups were collected to measure the TNF-α and IL-10 concentrations, and detect the influence on neuronal survival. Finally, TTC staining and modified Neurological Severity Score (mNSS) were used to determine the effects of MCM on ischemic stroke volume and neurological functions.

Results: The 10 Hz rTMS inhibited ischemia/reperfusion induced M1 microglia and significantly increased let-7b-5p level in microglia. HMGA2 was predicted and proved to be the target protein of let-7b-5p. HMGA2 and its downstream NF-κB signaling pathway were inhibited by rTMS. Microglia culture medium (MCM) collected from rTMS treated microglia contained lower TNF-α concentration but higher IL-10 concentration than no rTMS treated MCM, reducing ischemic volumes and neurological deficits of MCAO mice. However, knockdown of let-7b-5p by antagomir reversed rTMS effects on microglia phenotype and associated HMGA/NF-κB activation and neurological recovery.

Conclusion: High-frequency rTMS could alleviate ischemic stroke injury through inhibiting M1 microglia polarization via regulating let-7b-5p/HMGA2/NF-κB signaling pathway in MCAO models.
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http://dx.doi.org/10.1186/s12868-022-00735-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351069PMC
August 2022

VCAM-1-binding peptide targeted cationic liposomes containing NLRP3 siRNA to modulate LDL transcytosis as a novel therapy for experimental atherosclerosis.

Metabolism 2022 Jul 30;135:155274. Epub 2022 Jul 30.

Department of Endocrinology, Institute of Geriatric Medicine, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430077, China; Department of Pharmacology, the Key Laboratory of Drug Target Researches and Pharmacodynamics Evaluation of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address:

Background: Activation of NLRP3 inflammasome accelerates the formation of atherosclerotic plaques. Here, we evaluated the effects of inflammation on the expression of the NLRP3 inflammasome in endothelial cells (ECs).

Methods: The effect of TNF-α on transcytosis of LDL was measured. VCAM-1 binding peptide targeting cationic liposomes (PCLs) were prepared as siRNA vectors. Methylated NLRP3 siRNA was encapsulated into the PCLs to knock down NLRP3 in vitro and in vivo. In rats with partial carotid ligation, TNF-α-induced LDL retention in the carotid artery endothelium was observed. In ApoE mice, NLRP3 siRNA-PCLs were injected intravenously to observe their effect on the formation of atherosclerosis.

Results: Our results showed that TNF-α upregulated NLRP3 in ECs, promoting the assembly of the NLRP3 inflammasome and processing of pro-IL-1β into IL-1β. Moreover, TNF-α accelerated LDL transcytosis in ECs. Knockdown of NLRP3 prevented TNF-α-induced NLPR3 inflammasome/IL-1β signaling and LDL transcytosis. Using optimized cationic liposomes to encapsulate methylated NLRP3 siRNA, resulting in targeting of VCAM-1-expressing ECs, to knockdown NLRP3, TNF-α-induced NLRP3 inflammasome activation and LDL transcytosis were prevented. Using the partial carotid ligation as an atherosclerosis rat model, we found that local administration of NLRP3 siRNA-PCLs efficiently knocked down NLPR3 expression in the carotid endothelium and dramatically attenuated the deposition of atherogenic LDL in carotid ECs in TNF-α-challenged rats. Furthermore, NLRP3 siRNA-PCLs were injected intravenously in ApoE mice, resulting in reduced plaque formation.

Conclusion: These findings established a novel strategy for targeting the NLRP3 inflammasome using NLRP3 siRNA-PCLs to interrupt LDL transcytosis, representing a potential novel therapy for atherosclerosis.
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http://dx.doi.org/10.1016/j.metabol.2022.155274DOI Listing
July 2022

Saponins of Marsdenia Tenacissima promotes apoptosis of hepatocellular carcinoma cells through damaging mitochondria then activating cytochrome C/Caspase-9/Caspase-3 pathway.

J Cancer 2022 4;13(9):2855-2862. Epub 2022 Jul 4.

School of Life Sciences, Anhui Medical University, Hefei, China.

Liver cancer is one of the most common cancers in the world and the second leading cause of death in cancer patients. There is an urgent need for an effective and less toxic treatment for liver cancer. Saponins of Marsdenia Tenacissima (SMT) as a potential anticancer drug has attracted extensive attention of researchers because of its effective biological activity. The effect of SMT on HepG2 Li-7 and L-02 cells was detected by CCK8 assay. At the same time, the apoptosis rate was detected by flow cytometer and laser confocal microscope, and the morphological changes of mitochondria were observed under electron microscope. The levels of bax, cytochrome c, caspase-9, caspase-3, cleaved caspase-3 and protein were detected using Western bolt. Finally, BALB/c was subcutaneously injected with H22 cells to form tumors, and SMT was intragastrically injected to detect the size of the transplanted tumor. SMT can induce apoptosis and reduce the size of transplanted tumor . Increases the rate of apoptosis through the cytochrome c pathway and regulates the expression of apoptosis-related proteins. These results suggest that SMT may be one of the potential candidates for the treatment of liver cancer.
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http://dx.doi.org/10.7150/jca.72601DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330464PMC
July 2022

Engineering monodispersed 2 nm SbS particles embedded in a porphyrin-based MOF-derived mesoporous carbon network an adsorption method to construct a high-performance sodium-ion battery anode.

Dalton Trans 2022 Jul 27. Epub 2022 Jul 27.

Department of Chemistry, Northeastern University, Shenyang 110819, People's Republic of China.

Sodium ion batteries (SIBs) are expected to replace lithium ion batteries (LIBs) as the next generation of large-scale energy storage applications because of their superior cost performance. However, the larger ionic radius of Na causes a remarkable volume expansion than that of Li during charge and discharge, which reduces the performance of the battery. In this work, we engineered a composite material in that monodispersed 2 nm SbS particles are uniformly loaded into a carbon matrix (SbS/CZM), which is obtained by carbonization of a zirconium-based MOF with adsorption of Sb. The obtained composite material has a high specific surface area in favor of mass transfer, and the porous structure can resist many volume changes in the circulation process. Moreover, the ultrafine SbS particles are well-distributed in the composite material, which increases the utilization of the active substance and is promising for the storage of Na. Based on its unique structure, the SbS/CZM composite shows a specific capacity of 550 mA h g at 100 mA g and an excellent cycling stability of 88.9% retention after 1000 cycles at 3 A g. The excellent electrochemical performance provides enlightenment for the rational design of hierarchical heterostructures for energy storage applications.
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http://dx.doi.org/10.1039/d2dt01898eDOI Listing
July 2022

Cost-effectiveness analysis of prolonged-release fampridine to treat walking disability of multiple sclerosis in China.

J Comp Eff Res 2022 Jul 27. Epub 2022 Jul 27.

School of International Pharmaceutical Business, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China.

This study evaluates the cost-effectiveness of adding prolonged-release (PR)-fampridine to best supportive care (BSC) versus BSC alone in adult multiple sclerosis patients with walking disability in China. A hybrid decision tree and Markov model from both the societal and healthcare perspectives were constructed. Parameters were derived from clinical trials of PR-fampridine, published sources and clinical expert interviews. Over a 10-year time horizon, adding PR-fampridine to BSC led to 0.15 quality-adjusted life year (QALY) gain and lower costs, with incremental cost-effectiveness ratios of -238,806 Chinese Yuan/QALY and -113,488 Chinese Yuan/QALY from the societal and healthcare perspectives, respectively. Compared with BSC alone, PR-fampridine plus BSC is considered an economically dominant strategy for the treatment of multiple sclerosis-related walking disability in China.
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http://dx.doi.org/10.2217/cer-2022-0109DOI Listing
July 2022

An Investigation for Skin Tissue Regeneration Enhancement/Augmentation by Curcumin-Loaded Self-Emulsifying Drug Delivery System (SEDDS).

Polymers (Basel) 2022 Jul 17;14(14). Epub 2022 Jul 17.

Irma Lerma Rangel College of Pharmacy, Texas A&M University, College Station, TX 77843, USA.

Diabetes, one of the global metabolic disorders, is often associated with delayed wound healing due to the elevated level of free radicals at the wound site, which hampers skin regeneration. This study aimed at developing a curcumin-loaded self-emulsifying drug delivery system (SEDDS) for diabetic wound healing and skin tissue regeneration. For this purpose, various curcumin-loaded SEDDS formulations were prepared and optimized. Then, the SEDDS formulations were characterized by the emulsion droplet size, surface charge, drug content/entrapment efficiency, drug release, and stability. In vitro, the formulations were assessed for the cellular uptake, cytotoxicity, cell migration, and inhibition of the intracellular ROS production in the NIH3T3 fibroblasts. In vivo, the formulations' wound healing and skin regeneration potential were evaluated on the induced diabetic rats. The results indicated that, after being dispersed in the aqueous medium, the optimized SEDDS formulation was readily emulsified and formed a homogenous dispersion with a droplet size of 37.29 ± 3.47 nm, surface charge of -20.75 ± 0.07 mV, and PDI value of less than 0.3. The drug content in the optimized formulation was found to be 70.51% ± 2.31%, with an encapsulation efficiency of 87.36% ± 0.61%. The SEDDS showed a delayed drug release pattern compared to the pure drug solution, and the drug release rate followed the Fickian diffusion kinetically. In the cell culture, the formulations showed lower cytotoxicity, higher cellular uptake, and increased ROS production inhibition, and promoted the cell migration in the scratch assay compared to the pure drug. The in vivo data indicated that the curcumin-loaded SEDDS-treated diabetic rats had significantly faster-wound healing and re-epithelialization compared with the untreated and pure drug-treated groups. Our findings in this work suggest that the curcumin-loaded SEDDS might have great potential in facilitating diabetic wound healing and skin tissue regeneration.
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http://dx.doi.org/10.3390/polym14142904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315559PMC
July 2022

Testing an Iron Oxide Nanoparticle-Based Method for Magnetic Separation of Nanoplastics and Microplastics from Water.

Nanomaterials (Basel) 2022 Jul 9;12(14). Epub 2022 Jul 9.

Department of Life Sciences, Texas A&M University, Corpus Christi, TX 78412, USA.

Nanoplastic pollution is increasing worldwide and poses a threat to humans, animals, and ecological systems. High-throughput, reliable methods for the isolation and separation of NMPs from drinking water, wastewater, or environmental bodies of water are of interest. We investigated iron oxide nanoparticles (IONPs) with hydrophobic coatings to magnetize plastic particulate waste for removal. We produced and tested IONPs synthesized using air-free conditions and in atmospheric air, coated with several polydimethylsiloxane (PDMS)-based hydrophobic coatings. Particles were characterized with scanning electron microscopy (SEM), transmission electron microscopy (TEM), superconducting quantum interference device (SQUID) magnetometry, dynamic light scattering (DLS), X-ray diffraction (XRD) and zeta potential. The IONPs synthesized in air contained a higher percentage of the magnetic spinel phase and stronger magnetization. Binding and recovery of NMPs from both salt and freshwater samples was demonstrated. Specifically, we were able to remove 100% of particles in a range of sizes, from 2-5 mm, and nearly 90% of nanoplastic particles with a size range from 100 nm to 1000 nm using a simple 2-inch permanent NdFeB magnet. Magnetization of NMPs using IONPs is a viable method for separation from water samples for quantification, characterization, and purification and remediation of water.
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http://dx.doi.org/10.3390/nano12142348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315505PMC
July 2022

Effects of Environmental Concentrations of Total Phosphorus on the Plankton Community Structure and Function in a Microcosm Study.

Int J Environ Res Public Health 2022 Jul 9;19(14). Epub 2022 Jul 9.

Key Laboratory of Pollution Process and Environmental Criteria of Ministry of Education and Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.

The excessive nutrients in freshwater have been proven to promote eutrophication and harmful algae blooms, which have become great threats to water quality and human health. To elucidate the responses of the plankton community structure and function to total phosphorus (TP) at environmental concentrations in the freshwater ecosystem, a microcosm study was implemented. The results showed that plankton communities were significantly affected by the TP concentration ≥ 0.1 mg/L treatments. In terms of community structure, TP promoted the growth of Cyanophyta. This effect was transmitted to the zooplankton community, resulting in the promotion of Cladocera growth from day 42. The community diversities of phytoplankton and zooplankton had been continuously inhibited by TP. The principal response curve (PRC) analysis results demonstrated that the species composition of phytoplankton and zooplankton community in TP enrichment treatments significantly ( ≤ 0.05) deviated from the control. For community function, TP resulted in the decline in phytoplankton photosynthesis. The chlorophyll fluorescence parameters were significantly inhibited when TP concentration reached 0.4 mg/L. In TP ≥ 0.1 mg/L treatments, the reductions in total phytoplankton abundances led to a continuous decrease in pH. This study can directly prove that the plankton community changes significantly when TP concentrations are greater than 0.1 mg/L and can help managers to establish specific nutrient management strategies for surface water.
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http://dx.doi.org/10.3390/ijerph19148412DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324525PMC
July 2022

Flooding-induced rhizosphere Clostridium assemblage prevents root-to-shoot cadmium translocation in rice by promoting the formation of root apoplastic barriers.

J Hazard Mater 2022 Jul 16;439:129619. Epub 2022 Jul 16.

Key Lab of Bio-Organic Fertilizer Creation, Ministry of Agriculture and Rural Affairs, Anhui Science and Technology University, Bengbu 233100, China; Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

Water managements are the most effective agricultural practices for restraining cadmium (Cd) uptake and translocation in rice, which closely correlated with rhizosphere assembly of beneficial microbiome. However, the role of the assemblage of specific microbiota in controlling root-to-shoot Cd translocation in rice remains scarcely clear. The aim of this study was to ascertain how water managements shaped rhizosphere microbiome and mediated root-to-shoot Cd translocation. To disentangle the acting mechanisms of water managements, we performed an experiment monitoring Cd uptake and transport in rice and changes in soil microbial communities in response to continuously flooding and moistening irrigation. Continuously flooding changed rhizosphere microbial communities, leading to the increased abundance of anaerobic bacteria such as Clostridium populations. Weighted gene co-expression network analysis (WGCNA) showed that a dominant OTU163, corresponding to Clostridium sp. CSP1, exhibited a strong negative correlation with root-to-shoot Cd translocation. An integrated analysis of transcriptome and metabolome further indicated that the Clostridium-secreted butyric acid was involved in the regulation of phenylpropanoid pathway in rice roots. The formation of endodermal suberized barriers and lignified xylems was remarkably enhanced in the Clostridium-treated roots, which led to more Cd retained in root cell wall and less Cd in the xylem sap. Collectively, our results indicate that the development of root apoplastic barriers can be orchestrated by beneficial Clostridium strains that are assembled by host plants grown under flooding regime, thereby inhibiting root-to-shoot Cd translocation.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129619DOI Listing
July 2022

Dihydroartemisinin Attenuates Hypoxic Pulmonary Hypertension via the Downregulation of miR-335 Targeting .

DNA Cell Biol 2022 Aug 19;41(8):750-767. Epub 2022 Jul 19.

Division of Pulmonary Medicine, Key Laboratory of Heart and Lung, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Dihydroartemisinin (DHA) is a traditional antimalarial drug. DHA plays a crucial role in preventing pulmonary hypertension (PH); however, its regulatory function on microRNAs (miRNAs) in PH remains unclear. This study aimed to investigate whether DHA exerts its protective functions by regulating miR-335 in PH. Hypoxia-induced PH models were induced both and . Mice were treated with various concentrations of DHA, and pulmonary arterial smooth muscle cells (PASMCs) were treated with DHA, miR-335 inhibitor, miR-335 mimic, or Van Gogh-like 2 () plasmid. The expression of miR-335 and , pulmonary arterial remodeling index; right ventricular hypertrophy index; and proliferation and migration indexes were measured. DHA improved pulmonary vascular remodeling and alleviated PH . miRNA sequencing and real-time PCR results further show that the increase in hypoxia-induced miR-335 was avoided by DHA administration, and miR-335 increased the hypoxia-induced PASMC proliferation and migration. MiRNA databases and dual-luciferase reporter assay show that miR-335 directly targets , and decreased the hypoxia-induced PASMC proliferation and migration. The miR-335 inhibitor failed to inhibit hypoxia-induced proliferation and migration upregulation in knockdown PASMCs, and the effect of DHA can be blocked by miR-335 upregulation. In hypoxic PH, MiR-335 is increased, whereas is decreased. MiR-335 can significantly promote the hypoxia-induced proliferation and migration of PASMCs by targeting the gene. DHA effectively reverses the hypoxia-induced upregulation of miR-335 expression, avoiding the miR-335-mediated downregulation of and thereby promoting the expression of to prevent PH.
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http://dx.doi.org/10.1089/dna.2021.1113DOI Listing
August 2022

Deep Learning for Additive Screening in Perovskite Light-Emitting Diodes.

Angew Chem Int Ed Engl 2022 Jul 20:e202209337. Epub 2022 Jul 20.

Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, 211816, China.

Additive engineering with organic molecules is of critical importance for achieving high-performance perovskite optoelectronic devices. However, experimentally finding suitable additives is costly and time consuming, while conventional machine learning (ML) is difficult to predict accurately due to the limited experimental data available in this relatively new field. Here, we demonstrate a deep learning method that can predict the effectiveness of additives in perovskite light-emitting diodes (PeLEDs) with a high accuracy up to 96 % by using a small dataset of 132 molecules. This model can maximize the information of the molecules and significantly mitigate the duplicated problem that usually happened with previous models in ML for molecular screening. Very high efficiency PeLEDs with a peak external quantum efficiency up to 22.7 % can be achieved by using the predicated additive. Our work opens a new avenue for further boosting the performance of perovskite optoelectronic devices.
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http://dx.doi.org/10.1002/anie.202209337DOI Listing
July 2022

Selective corticofugal modulation on sound processing in auditory thalamus of awake marmosets.

Cereb Cortex 2022 Jul 18. Epub 2022 Jul 18.

Department of Neurology of the Second Affiliated Hospital, College of Biomedical Engineering and Instrument Science, Interdisciplinary Institute of Neuroscience and Technology, Zhejiang University, School of Medicine, Zhejiang University, 268 Kaixuan Road, Science Building, Room 206, Hangzhou, Zhejiang 310020, China.

Cortical feedback has long been considered crucial for the modulation of sensory perception and recognition. However, previous studies have shown varying modulatory effects of the primary auditory cortex (A1) on the auditory response of subcortical neurons, which complicate interpretations regarding the function of A1 in sound perception and recognition. This has been further complicated by studies conducted under different brain states. In the current study, we used cryo-inactivation in A1 to examine the role of corticothalamic feedback on medial geniculate body (MGB) neurons in awake marmosets. The primary effects of A1 inactivation were a frequency-specific decrease in the auditory response of most MGB neurons coupled with an increased spontaneous firing rate, which together resulted in a decrease in the signal-to-noise ratio. In addition, we report for the first time that A1 robustly modulated the long-lasting sustained response of MGB neurons, which changed the frequency tuning after A1 inactivation, e.g. some neurons are sharper with corticofugal feedback and some get broader. Taken together, our results demonstrate that corticothalamic modulation in awake marmosets serves to enhance sensory processing in a manner similar to center-surround models proposed in visual and somatosensory systems, a finding which supports common principles of corticothalamic processing across sensory systems.
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http://dx.doi.org/10.1093/cercor/bhac278DOI Listing
July 2022

Orexin neurons inhibit sleep to promote arousal.

Nat Commun 2022 Jul 18;13(1):4163. Epub 2022 Jul 18.

Department of Neurology, Division of Sleep Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, 02215, USA.

Humans and animals lacking orexin neurons exhibit daytime sleepiness, sleep attacks, and state instability. While the circuit basis by which orexin neurons contribute to consolidated wakefulness remains unclear, existing models posit that orexin neurons provide their wake-stabilizing influence by exerting excitatory tone on other brain arousal nodes. Here we show using in vivo optogenetics, in vitro optogenetic-based circuit mapping, and single-cell transcriptomics that orexin neurons also contribute to arousal maintenance through indirect inhibition of sleep-promoting neurons of the ventrolateral preoptic nucleus. Activation of this subcortical circuit rapidly drives wakefulness from sleep by differentially modulating the activity of ventrolateral preoptic neurons. We further identify and characterize a feedforward circuit through which orexin (and co-released glutamate) acts to indirectly target and inhibit sleep-promoting ventrolateral preoptic neurons to produce arousal. This revealed circuitry provides an alternate framework for understanding how orexin neurons contribute to the maintenance of consolidated wakefulness and stabilize behavioral state.
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http://dx.doi.org/10.1038/s41467-022-31591-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293990PMC
July 2022

Optimized Mo-doped IrO anode for efficient degradation of refractory sulfadiazine.

Environ Sci Pollut Res Int 2022 Jul 18. Epub 2022 Jul 18.

School of Resources and Environmental Engineering, Environmental Protection Key Laboratory of Environmental Risk, East China University of Science and Technology, 130 Mei long Road, Shanghai, 200237, People's Republic of China.

Electrochemical advanced oxidation processes (EAOPs) is considered to be an efficacious method to degrade antibiotics. However, the performance of the anode has become the main limiting factor of this technology. In this study, due to the electron-deficient characteristics and the improvement of OER performance of Mo, we chose to use thermal decomposition to incorporate Mo into IrO to prepare anodes with industrial applicability. Under the optimal ratio of Ir to Mo is 7:3, (IrMo)O electrode's particular pore structure can expose more active sites and create a channel for the transportation of electrons, thereby promoting the formation of free radicals and degrading pollutants more efficiently. (IrMo)O electrode also has a higher mass activity (6.332 A g, three times that of the IrO electrode) and a larger electrochemical active area (ECSA, 375.43 cm, seven times that of the IrO electrode). In addition, the optimal conditions of (IrMo)O electrode for degrading sulfadiazine(SDZ) were explored, which achieved a higher removal than traditional electrodes (90% removal within 4 h) when the Ti plate was the substrate. Through the intermediate products of SDZ degradation and related literatures, two possible degradation pathways of SDZ were speculated. This research provides a new type of anode catalyst for the degradation of sulfonamide antibiotics, which is possible for industrial application.
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http://dx.doi.org/10.1007/s11356-022-22033-9DOI Listing
July 2022

Insulin-Like Growth Factor 2 mRNA-Binding Protein 3 and Its Related Molecules as Potential Biomarkers in Small-Cell Lung Cancer.

Biomed Res Int 2022 7;2022:5774339. Epub 2022 Jul 7.

Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.

Background: Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) plays a key role in tumorigenesis and tumor progression. Lung cancer is the leading cause of cancer-related death in men and women all over the world. However, the relationship between IGF2BP3 and small-cell lung cancer (SCLC) has not been reported yet.

Methods: SCLC and normal samples (GSE19945 and GSE149507) were obtained in the Gene Expression Omnibus (GEO) dataset. Differential genes were screened by R software, and functional analysis and signal pathway enrichment analysis were carried out. In addition, we used the survival analysis database to analyze the relationship between prognosis and gene expression. Besides, immunohistochemistry (IHC) and quantitative real-time PCR (qPCR) were used for further research.

Results: Five differentially expressed miRNAs and 9 differentially expressed mRNAs were selected by using R software. Survival analysis database results show that C7, CLIC5, PRDX1, IGF2BP3, and LDB2 were related the overall survival of patients with SCLC. Furthermore, multivariate analysis included that IGF2BP3 was independent risk factors for SCLC patients. Besides, gene function and signal pathway enrichment analysis showed that differentially expressed miRNAs were involved in the process of tumorigenesis and development. Furthermore, IHC and qPCR outcomes showed that the expression level of hsa-miR-182, hsa-miR-183, and IGF2BP3 was differentially expressed in normal lung tissues (NLTs) and SCLC tissues (SCLCTs).

Conclusions: Our results concluded that hsa-miR-182, hsa-miR-183, and IGF2BP3 may take part in the development of SCLC.
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http://dx.doi.org/10.1155/2022/5774339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283012PMC
July 2022

Analysis and Application of Language Environment Function from the Perspective of English Linguistics.

Authors:
Lin Zhu

J Environ Public Health 2022 8;2022:1757002. Epub 2022 Jul 8.

School of Foreign Study, Henan Polytechnic University, Jiaozuo 454000, China.

In recent years, more and more questions have been raised about the idea that a sentence alone can fully analyze the semantics without considering the context. When we talk about the acceptability of a sentence, we immediately and naturally think of the context in which the sentence can be used correctly. In the current English classroom teaching, teachers follow the traditional teaching procedures too much: first give detailed explanations, and then carry out mechanical exercises. The lack of flexible teaching methods and the creation of contexts affects the improvement of students' communicative ability. During my long-term learning of English, I have considered the following questions: how to make use of the function of context to make English learning diverse and challenging; how to create a variety of practical forms, such as creating a way to make students interested and able to participate in learning, in order to improve students' language communication ability. This paper analyzes the current situation of English teaching, expounds the concept and classification of context and language application, summarizes the applied linguistics theory and related theory, and analyzes the context of English linguistics horizon function and application. This study combines context with English learning, and English learning "how to create and use context," so as to improve students' communicative competence.
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http://dx.doi.org/10.1155/2022/1757002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286887PMC
July 2022

Calycosin suppresses colorectal cancer progression by targeting ERβ, upregulating PTEN, and inhibiting PI3K/Akt signal pathway.

Cell Biol Int 2022 Sep 16;46(9):1367-1377. Epub 2022 Jul 16.

Department of Pharmacology, School of Pharmacy, Chengdu Medical College, Chengdu, Sichuan, China.

High intake of phytoestrogen has been reported to be associated with the prevention of colorectal cancer (CRC). Calycosin belongs to the phytoestrogen that has been shown to suppress CRC cells in our previous study. However, its anticancer activity and molecular mechanisms have not been elucidated. In this study, we analyzed the effect of calycosin on the viability and apoptosis of human CRC HCT116 and SW480 cells via MTT assay, flow cytometry assay, and caspase-3/7 activity assay. The protein expressions of estrogen receptor β (ERβ), PTEN, and PI3K/Akt signal pathways were determined by Western blot analysis. And then, the alterations of biological behavior in CRC cells transfected with ERβ siRNA were analyzed. Mouse xenograft models were further performed to detect the antitumor effect in vivo. The results show that calycosin reduces CRC cell viability, induces cell apoptosis, and suppresses xenograft tumor growth. The protein expressions of ERβ and PTEN are significantly upregulated following calycosin treatment, whereas p-AKT/AKT ratio and Bcl-2 level are downregulated. Suppressing ERβ with siRNA partially attenuates the reduction in viability and apoptosis induced by calycosin. Our results indicate that calycosin shows inhibitory effects on CRC cells, which might be obtained by targeting ERβ, upregulating PTEN, and inhibiting the PI3K/Akt signal pathway.
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http://dx.doi.org/10.1002/cbin.11840DOI Listing
September 2022

Phthalates in dormitory dust and human urine: A study of exposure characteristics and risk assessments of university students.

Sci Total Environ 2022 Jul 8;845:157251. Epub 2022 Jul 8.

Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China. Electronic address:

Phthalate diesters (PAEs) are prevalent and potentially toxic to human health. The university dormitory represents a typical and relatively uniform indoor environment. This study evaluated the concentrations of phthalate monoesters (mPAEs) in urine samples from 101 residents of university status, and the concentrations of PAEs in dust collected from 36 corresponding dormitories. Di-(2-ethylhexyl) phthalate (DEHP, median: 68.0 μg/g) was the major PAE in dust, and mono-ethyl phthalate (47.9 %) was the most abundant mPAE in urine. The levels of both PAEs in dormitory dust and mPAEs in urine were higher in females than in males, indicating higher PAE exposure in females. Differences in lifestyles (dormitory time and plastic product use frequency) may also affect human exposure to PAEs. Moreover, there were significant positive correlations between the estimated daily intakes of PAEs calculated by using concentrations of PAEs in dust (EDI) and mPAEs in urine (EDI), suggesting that PAEs in dust could be a significant source of human exposure to PAEs. The value of EDI/EDI for low molecular weight PAEs (3-6 carbon atoms in their backbone) was lower than that of high molecular weight PAEs. The contribution rate of various pathways to PAE exposure illustrated that non-dietary ingestion (87.8 %) was the major pathway of human exposure to PAEs in dust. Approximately 4.95 % of university students' hazard quotients of DEHP were >1, indicating that there may be some health risks associated with DEHP exposure among PAEs. Furthermore, it is recommended that some measures be taken to reduce the production and application of DEHP.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157251DOI Listing
July 2022

Effect of semaglutide and liraglutide in individuals with obesity or overweight without diabetes: a systematic review.

Ther Adv Chronic Dis 2022 4;13:20406223221108064. Epub 2022 Jul 4.

Center of Liver Diseases, Beijing Ditan Hospital, Capital Medical University, No. 8, Jingshun East Street, Chaoyang District, Beijing 100015, China.

Background & Objective: Although data on the effects of liraglutide and semaglutide in patients with diabetes have been reviewed, their therapeutic outcomes in obese/overweight individuals without diabetes have not been summarized. We conducted a systematic review to evaluate their effects on the latter population.

Methods: We searched the PubMed, EMBASE, Cochrane, CNKI, and Wanfang databases. Studies regarding obese/overweight adults without diabetes treated with liraglutide/semaglutide compared with other active agents or placebos were accessed. The primary outcomes were the proportions of adults with at least 5% and 10% weight reduction. The secondary outcomes included metabolic indicators and adverse events.

Results: Eighteen studies with 10,938 obese/overweight adults without diabetes were included. When stratified by the categories of at least 5% and 10% weight loss, the pooled data showed medians 27.7% and 10.3% of control groups versus 65.3% and 30.7% of liraglutide 3 mg once daily, respectively; whereas medians 47.6% and 20.4% of control groups vs 86.6% and 75.3% of semaglutide 2.4 mg once weekly were found in the two categories, respectively. Both agents either improved or had no impact on lipid or glycemia. Liraglutide or semaglutide therapy had discontinuation rates of 2.4%-11.4% which overlapped with 0.7%-8.6% in control groups. The frequency of adverse events was comparable between the treatment groups and the control groups (66.5%-95.8% vs 46.9%-96.1%), which were mild to moderate graded by studies.

Conclusion: Liraglutide and semaglutide therapy led to a clinically relevant (⩾5%) weight loss of 48.2%-88.7% among obese/overweight adults without diabetes. Both liraglutide and semaglutide are associated with weight loss and are well-tolerated.
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http://dx.doi.org/10.1177/20406223221108064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260566PMC
July 2022

Higher CO Assimilation in Selected Rice Recombinant Inbred Lines Is Driven by Higher CO Diffusion and Light Use Efficiency Related to Leaf Anatomy and Mesophyll Cell Density.

Front Plant Sci 2022 9;13:915050. Epub 2022 Jun 9.

Key Laboratory of Rice Biology & Genetic Breeding in Northeast China (Ministry of Agriculture and Rural Areas), Rice Research Institute, Shenyang Agricultural University, Shenyang, China.

Leaf anatomy determining the light distribution within the leaf and exerting influence on CO diffusion is considered to have dramatic potential for photosynthesis performance increase. In this study, we observed that two rice recombinant inbred lines, H138 and H217 (RILF plants from Sasanishiki × IRAT10), have higher net CO assimilation (An) than their parent Sasanishiki due mainly to the improvement of leaf anatomy. Our results showed that An positively correlated with anatomy traits' mesophyll cell number per cross-sectional area (NO/A) and mesophyll area (A). NO./A exert direct and indirect effects on An. Compared to Sasanishiki flag leaves, IRAT10, H138, and H217 have higher mesophyll cell numbers. Simultaneously, higher chlorophyll content and expression of genes encoding the light-harvesting protein of PSII and PSI (, , and , , ) were recorded in IRAT10, H138, and H217, which facilitates light use efficiency. Higher electron transport rate and RuBP concentration were recorded in IRAT10, H138, and H217 flag leaves. Retinoblastoma-related gene (), exerting effects on mesophyll cell density, can be used to modify leaf anatomy for improving leaf photosynthesis. Additionally, higher stomatal conductance and mesophyll conductance were also recorded in H138 and H217 than in Sasanishiki. Furthermore, we modeled mesophyll conductance through anatomical traits, and the results revealed that chloroplast thickness was the dominant factor restricting CO diffusion within mesophyll cells rather than cell wall thickness. Higher RuBP content accompanied by higher CO concentration within the carboxylation set in H138 and H217 flag leaves contributed to higher CO assimilation.
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http://dx.doi.org/10.3389/fpls.2022.915050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9261980PMC
June 2022

A detailed assessment of liver function in patients with hepatocellular carcinoma via the modified albumin-bilirubin (mALBI) grade.

Am J Cancer Res 2022 15;12(6):2711-2720. Epub 2022 Jun 15.

State Key Laboratory of Oncology in South China and Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center Guangzhou, Guangdong, China.

Recently, the albumin-bilirubin (ALBI) score, a continuous index consisting of only albumin and bilirubin, has been developed for objectively assessing liver function in patients with hepatocellular carcinoma (HCC). However, the ALBI score was arbitrarily categorized into three ALBI grades based on two artificially predetermined cutoff points with no explanation and statistical grounds, causing a considerable loss of discriminatory ability. This study aims to propose a modified ALBI (mALBI) grade for offering a detailed evaluation of hepatic reserve and specify its role during clinical practice in the HCC setting. The study population comprised 3540 HCC patients treated with mainstream therapies including hepatectomy (n=2056), thermal ablation (n=550), and transcatheter arterial chemoembolization (n=934) from 2002 to 2017. The ALBI score was stratified into four mALBI grades through a recently proposed statistical method aiming to select the optimal cutoff points of a continuous predictive variable by maximizing the discriminative ability in a multivariable Cox regression model. The mALBI grade had an overall better discriminatory ability than the ALBI grade in predicting overall survival through Harrell's C-index (0.614 vs. 0.598, P<0.001). Both visual inspections of Kaplan-Meier curves and calculation of hazard ratios displayed a more subtle evaluation of liver function using the mALBI grade. Moreover, the newly identified cut-point (ALBI score = -2.29) between the mALBI grade 2a and 2b was much closer to a 30% retention rate of indocyanine green at 15 minutes, an indicator for the performance of a subsegmentectomy. The newly proposed mALBI grade provides a more subtle assessment of liver function to guide clinical decision-making and predicts the prognosis of HCC patients more accurately than the original ALBI grade.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251706PMC
June 2022

Corazonin signaling modulates the synthetic activity of male accessory gland in Grapholita molesta.

Int J Biol Macromol 2022 Jul 7;216:446-455. Epub 2022 Jul 7.

Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China. Electronic address:

Although neuropeptide corazonin (Crz) has been identified in numerous insect species, the research about its function in regulation of reproduction is still in its infancy. Herein, we characterized the Crz (GmolCrz) and its receptor (GmolCrzR) to investigate their reproductive function in Grapholita molesta. Both molecular docking result and cell-based receptor activity assay showed that GmolCrz could interact with GmolCrzR. Additionally, spatial expression patterns of GmolCrz and GmolCrzR in males were evaluated. Knockdown of GmolCrz or GmolCrzR significantly lengthened copulation duration and decreased fertility in males. In these males, we found that the production of sperm was normal, while the content of accessory gland proteins (Acps) in the accessory gland (AG) was strongly diminished. Furthermore, knockdown of GmolCrz or GmolCrzR in males had no effect on sperm and Acps transfer to females. RNA-seq and gene expression analyses further confirmed that genes involved in serine-type endopeptidase activity were significantly downregulated in the AG upon GmolCrzR knockdown. Finally, sperm activation assays demonstrated that this process was disrupted in the spermatophore of females mated with GmolCrz or GmolCrzR knockdown males, which may cause the decreased fertility in males. Our findings provide new insights into the functions of Crz signaling in a Lepidopteran insect.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.025DOI Listing
July 2022

Quantification-Promoted Discovery of Glycosylated Exosomal PD-L1 as a Potential Tumor Biomarker.

Small Methods 2022 Jul 10:e2200549. Epub 2022 Jul 10.

The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian, 361005, China.

Exosomal programmed cell death ligand 1 (exoPD-L1) has emerged as a promising biomarker for cancer diagnosis and immunotherapy outcome prediction. However, the existing quantitation methods are incapable of addressing the heterogeneity of exoPD-L1 glycosylation, which has been demonstrated to be the institutional basis for PD-L1/PD-1 interaction and the crucial participant in inhibiting the activity of CD8 T cells. Herein, an aptamer- and lectin-induced proximity ligation assay combined with quantitative real-time polymerase chain reaction for precise quantitation of glycosylated exoPD-L1 is developed. Leveraging the metabolism-free lectin labeling of glycosylation, the glycosylation-independent aptamer tagging of PD-L1, and excellent selectivity of dual-recognition, this method enables glycosylated exoPD-L1 quantitation with high sensitivity and selectivity in a wash-free manner. As a result, this method is able to distinguish the levels of glycosylated exoPD-L1 between healthy donors and cancer patients with sensitivity and specificity of 100%. Compared with the total circulating exoPD-L1 level, glycosylated exoPD-L1 is for the first time identified to be a more reliable biomarker for tumor diagnosis. Overall, this strategy holds a great potential for revealing the significance of exoPD-L1 glycosylation and converting glycosylated exoPD-L1 into a reliable clinical indicator.
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http://dx.doi.org/10.1002/smtd.202200549DOI Listing
July 2022

Negative pressure wound therapy to decrease surgical site infection in patients undergoing pancreaticoduodenectomy: A systematic review and meta-analysis.

Asian J Surg 2022 Jul 6. Epub 2022 Jul 6.

Department of General Surgery (Hepatobiliary Surgery), The Affiliated Hospital of Southwest Medical University, Taiping Street No.25, Luzhou, 646000, Sichuan, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.06.020DOI Listing
July 2022

Understanding the Palatability, Flavor, Starch Functional Properties and Storability of Indica-Japonica Hybrid Rice.

Molecules 2022 Jun 22;27(13). Epub 2022 Jun 22.

Key Laboratory of Agro-Products Processing Ministry of Agriculture, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

The rice quality and starch functional properties, as well as the storability of three YY-IJHR cultivars, which included YY12 (biased japonica type YY-IJHR), YY1540 (intermedius type YY-IJHR) and YY15 (biased indica type YY-IJHR), were studied and compared to N84 (conventional japonica rice). The study results suggested that the three YY-IJHR varieties all had greater cooking and eating quality than N84, as they had lower amylose and protein content. The starch of YY-IJHR has a higher pasting viscosity and digestibility, and there was a significant difference among the three YY-IJHR cultivars. Rice aroma components were revealed by GC-IMS, which indicated that the content of alcohols vola-tile components of YY-IJHR were generally lower, whereas the content of some aldehydes and esters were higher than N84. In addition, YY-IJHR cultivars' FFA and MDA contents were lower, which demonstrated that YY-IJHR had a higher palatability and storability than those of N84 in fresh rice and rice stored for 12 months. In conclusion, this study suggested that YY-IJHR had better rice quality and storability than N84. PCA indicated that the grain quality and storability of YY12 and YY15 were similar and performed better than YY1540, while the aroma components and starch functional properties of YY-IJHR cultivars all had significant differences.
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http://dx.doi.org/10.3390/molecules27134009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268750PMC
June 2022
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