Publications by authors named "Lin Xiao"

1,120 Publications

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Pyridoxal Isonicotinoyl Hydrazone Improves Neurological Recovery by Attenuating Ferroptosis and Inflammation in Cerebral Hemorrhagic Mice.

Biomed Res Int 2021 8;2021:9916328. Epub 2021 Sep 8.

Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.

Ferroptosis and inflammation induced by cerebral hemorrhage result in an excessive inflammatory response and irreversible neuronal injury. Alleviating ferroptosis might be an effective way to prevent neuroinflammatory injury and promote neural functional recovery. Pyridoxal isonicotinoyl hydrazine (PIH), a lipophilic iron-chelating agent, has been reported to reduce excess iron-induced cytotoxicity. However, whether PIH could ameliorate the effects of hemorrhagic stroke is not completely understood. In the present study, the preventive effects of PIH in an intracerebral hemorrhage (ICH) mouse model were investigated. Neurological score, rotarod test, and immunofluorescence around the hematoma were assessed to evaluate the effects of PIH on hemorrhagic injury. The involvement of ferroptosis and inflammation was also examined in vitro to explore the underlying mechanism. Results showed that administration of PIH prevented neuronal cell death and reduced lipid peroxidation in Erastin-treated PC-12 cells. In vivo, mice treated with PIH after ICH attenuated neurological deficit scores. Additionally, we found PIH reduced ROS production, iron accumulation, and lipid peroxidation around the hematoma peripheral tissue. Meanwhile, ICH mice treated with PIH showed an upregulation of the key ferroptosis enzyme, glutathione peroxidase 4, and downregulation of cyclooxygenase-2. Moreover, PIH administration inhibited proinflammatory polarization and reduced interleukin-1 beta and tumor necrosis factor alpha in ICH mice. Collectively, these results demonstrated that PIH protects mice against hemorrhage stroke, which was associated with mitigation of inflammation and ferroptosis.
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http://dx.doi.org/10.1155/2021/9916328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445720PMC
September 2021

Generation of a vector light field based on polarization holography.

Opt Lett 2021 Sep;46(18):4542-4545

We propose a simple and effective method for generating a vector light field based on the faithful reconstruction (FR) effect of polarization holography, where the arbitrary linear polarization waves can be faithfully reconstructed by the polarization-sensitive recording media. The scheme incorporates the tunable and switchable dynamics exposure system to manufacture vector beams. By regulating the velocity and the initial polarization through the angle-aperture and the half-wave plate in the dynamics exposure system, the generated optical element can convert a linear polarization wave into a vector beam. We have analyzed the feasibility in theory and demonstrated the generation of the vector beams experimentally which shows good agreement with the theoretical simulations.
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http://dx.doi.org/10.1364/OL.438070DOI Listing
September 2021

Analyzing the synergistic adverse effects of BPA and its substitute, BHPF, on ulcerative colitis through comparative metabolomics.

Chemosphere 2021 Sep 5;287(Pt 2):132160. Epub 2021 Sep 5.

Laboratory of Environmental Pollution and Integrative Omics, Guilin Medical University, Guilin, PR China. Electronic address:

Ulcerative colitis (UC) is an inflammatory bowel disease (IBD) that causes long-term inflammation and ulcers in the colon and rectum. Approximately 3 million adults were diagnosed with IBD in the US in 2015, and its incidence rate is estimated to increase by 4-6 times in 2030. Industrial pollutants are largely responsible for this significant increase in UC cases. Several epidemiological and animal studies have demonstrated the correlation between pollutants and gastrointestinal diseases, but detailed molecular mechanisms responsible for adverse effects of environmental pollutants on UC are still unknown. In the present study, we used a dextran sulfate sodium (DSS)-induced colitis mouse model, comparative metabolomics analysis, and systematic bioinformatics analysis to delineate the synergistic adverse effects of bisphenol A (BPA) and its substitute fluorene-9-bisphenol (BHPF) on UC. Subsequently, a significant alteration in gut metabolites was observed by the BPA and BHPF treatments. Furthermore, the bioinformatics analysis indicated deregulation of sugar and fatty acid metabolisms in the DSS-induced colitis model by the BPA and BHPF treatments, respectively. Additionally, both the treatments induced an inflammatory response in the model. Particularly, some DSS-deregulated metabolites, which play important roles in gut inflammation, were synergistically induced or reduced by the BPA and BHPF treatments. To the best knowledge of the authors, the synergistic adverse effects of the BPA and BHPF treatments on UC were demonstrated for the first time through gut metabolism alterations. Therefore, the present study provides novel insights in the role of environmental pollutants, such as BPA and BHPF, in UC development.
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http://dx.doi.org/10.1016/j.chemosphere.2021.132160DOI Listing
September 2021

Detrimental effects of microplastic exposure on normal and asthmatic pulmonary physiology.

J Hazard Mater 2021 08 11;416:126069. Epub 2021 May 11.

The Department of Respiratory Diseases and Critic Care Unit, Shenzhen Institute of Respiratory Disease, Shenzhen Key Laboratory of Respiratory Disease, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen 518020, China; Post-Doctoral Scientific Research Station of Basic Medicine, Jinan University, Guangzhou 510632, China. Electronic address:

Concerns that airborne microplastics (MP) may be detrimental to human health are rising. However, research on the effects of MP on the respiratory system are limited. We tested the effect of MP exposure on both normal and asthmatic pulmonary physiology in mice. We show that MP exposure caused pulmonary inflammatory cell infiltration, bronchoalveolar macrophage aggregation, increased TNF-α level in bronchoalveolar lavage fluid (BALF), and increased plasma IgG1 production in normal mice. MP exposure also affected asthma symptoms by increasing mucus production and inflammatory cell infiltration with notable macrophage aggregation. Further, we found co-labeling of macrophage markers with MP incorporating fluorescence, which indicates phagocytosis of the MP by macrophages. A comparative transcriptomic analysis showed that MP exposure altered clusters of genes related to immune response, cellular stress response, and programmed cell death. A bioinformatics analysis further uncovered the molecular mechanism whereby MP stimulated production of tumor necrosis factor and immunoglobulins to activate a group of transmembrane B-cell antigens, leading to the modulation of cellular stress and programmed cell death in the asthma model. In summary, we show that MP exposure had detrimental effects on the respiratory system in both healthy and asthmatic mice, which calls for urgent discourse and action to mitigate environmental microplastic pollutants.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126069DOI Listing
August 2021

Management of nipple pain or trauma in breastfeeding mothers in a hospital setting: a best practice implementation project.

JBI Evid Implement 2021 Jan 19;19(3):236-244. Epub 2021 Jan 19.

PR China Southern Centre for Evidence Based Nursing and Midwifery Practice: A Joanna Briggs Institute Centre of Excellence, School of Nursing, Southern Medical University, Guangzhou, PR China.

Introduction And Aims: Breastfeeding is one of the best ways to ensure healthy growth and development of an infant. Nipple pain and trauma are common complications associated with breastfeeding, which render it difficult for the mother to continue breastfeeding. The aim of this project was to promote evidence-based practice in the management of nipple pain or trauma for breastfeeding mothers in a large tertiary hospital in China.

Methods: The project was conducted using the Joanna Briggs Institute framework and Practical Application of Clinical Evidence System. Six audit criteria were applied in baseline and follow-up audits to assess compliance in practice with best practice recommendations. The Getting Research into Practice tool was used to help analyze barriers to the fulfillment of each audit criterion based on the baseline audit results. Implementation strategies were discussed and then undertaken to overcome the barriers and bring changes to the current practice.

Results: The baseline audit results demonstrated poor compliance in nipple pain or trauma management practice with all of the six criteria. Significant improvements were achieved in the follow-up audit. Specifically, the compliance rate increased from 15 to 100% for criterion 1 and from 2 to 93% for criterion 2. For criteria 3, 4, 5 and 6, the compliance rate increased from 0 to 90, 85, 100 and 86%, respectively.

Conclusion: The current evidence-based implementation project was successfully conducted and achieved great improvement in the management of nipple pain or trauma associated with breastfeeding. Continuous effort is required to maintain changes and further improve practice.
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http://dx.doi.org/10.1097/XEB.0000000000000272DOI Listing
January 2021

[Effects of different hypoxic concentrations on biological characteristics of human dental pulp stem cells in vitro].

Shanghai Kou Qiang Yi Xue 2021 Jun;30(3):247-252

Department of Stomatology, Jiangmen Maternal and Child Health Care Hospital. Jiangmen 529000, China. E-mail:

Purpose: To investigate the effects of different hypoxic concentrations on biological characteristics of human dental pulp stem cells in vitro.

Methods: Impacted mandibular third molars were extracted from healthy individuals, and the dental pulp stem cells were cultured by tissue block enzyme digestion. Cells cultured under the conditions of 3%, 5% and 21% oxygen concentration for 7 days were set as 3% hypoxia group, 5% hypoxia group, and 21% nomoxia group, respectively. Flow cytometry was used to detect cell surface markers, cell cycle and apoptosis. Cell proliferation was measured by CCK-8 method. Transwell chamber assay was used to detect migration ability. Statistical analysis was completed by SPSS 20.0 software package.

Results: The expression rates of CD44, CD29 and D73 of the subculture cells were 97.25%, 99.36% and 99.60%, respectively. The proliferation ability of dental pulp stem cells was the strongest in 5% hypoxia group, and weakest in 3% hypoxia group, with significant difference(P<0.05). The apoptosis rate had no significant difference among various concentrations of oxygen(P>0.05). Compared with 21% nomoxia group, the proportion of dental pulp stem cells in G1 phase was significantly lower than that in 3% hypoxia group and 5% hypoxia group(P<0.05), and cell in S phase was significantly higher than that in 3% hypoxia group and 5% hypoxia group(P<0.05). The migration ability was the strongest in 3% hypoxia group, and weakest in 21% nomoxia group, with significant difference(P<0.05).

Conclusions: Different concentrations of hypoxia have great influence on the morphology, proliferation, migration and cell cycle of human dental pulp stem cells in vitro with little impact on cell apoptosis.
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June 2021

[Quality value transmitting of volatile components in personalized preparations:a case study of Menthae Haplocalycis Herba].

Zhongguo Zhong Yao Za Zhi 2021 Aug;46(15):3780-3788

Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine, Ministry of Education,Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.

Considering the characteristic chromatograms and quality value transmitting of three volatile components, this study investigated the changes in volatile components of Menthae Haplocalycis Herba in each heating process of personalized preparations and identified the critical control points for the application of volatile components from traditional Chinese medicine in such preparations. The characteristic chromatograms of volatile components in Menthae Haplocalycis Herba were established by gas chromatography, followed by the quantitative determination of three volatile components menthone, menthol, and piperitone and the comparison of retention rates of volatile components during the crushing, extraction, concentration and drying of preparation products and their change rules in characteristic peaks. The results showed that the volatile components of Menthae Haplocalycis Herba were reduced in each process. The loss rate was low in the crushing process when the volatile component peaks were present, but high in the extraction and concentration processes, manifested as the absence of partial component peaks and the presence of new component peaks. The changes in volatile components of Chuanxiong Chatiao Granule in the drying process were compared with those in Chuanxiong Chatiao Pill, both of which were prepared from the raw Menthae Haplocalycis Herba powder, and the findings demonstrated that Chuanxiong Chatiao Pill was superior to Chuanxiong Chatiao Granule. This study confirmed that the retention rates of volatile components in Menthae Haplocalycis Herba were mostly affected by the extraction and concentration processes, and the packing of preparations helped to reduce the loss of volatile components in Menthae Haplocalycis Herba powder, which has provided reference for the application of Chinese medicinal materials containing volatile components in the personalized preparations.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210525.303DOI Listing
August 2021

[Preparation of personalized traditional Chinese medicine condensed water pill without excipients based on correlation between concentrate viscosity and molding quality].

Zhongguo Zhong Yao Za Zhi 2021 Aug;46(15):3772-3779

Shanghai Traditional Chinese Medicine Health Service Collaborative Innovation Center,Shanghai University of Traditional Chinese Medicine Shanghai 201203,China.

To explore the correlation between concentrate viscosity and molding quality of personalized traditional Chinese medicine(TCM) condensed water pill, this study established a concentrate viscosity characterization method with rotational rheometry. Seven model prescriptions were respectively concentrated to different degrees and the viscosity of each concentrate was determined. The pre-sence of 'viscosity jump' in the middle stage of 'flag hanging' of all the model prescriptions implied that there might be an ideal viscosity range in the preparation of condensed water pill. The further study of 22 model prescriptions demonstrated that the optimum viscosity range of concentrate was 5-15 Pa·s(25 ℃) for approximately 82% of the prescriptions. About 18% of the prescriptions had a wide range, which might be caused by the high proportions of mineral and crustacean drugs in the crushing part and sugar and fibrous drugs in the decocting part. This study clarified the optimum viscosity range for concentrates of personalized TCM condensed water pills and achieved a preparation technology without any excipient, laying a foundation for the on-line control of the preparation.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210330.304DOI Listing
August 2021

[Establishment of prediction model for personalized water-paste pills based on classification of traditional Chinese medicine materials].

Zhongguo Zhong Yao Za Zhi 2021 Aug;46(15):3764-3771

Health Service Collaborative Innovation Center of Shanghai Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.

The purpose of the present study was to investigate the relationship of the classification of traditional Chinese medicine(TCM) materials with the suitable binder concentration and dosage in the preparation of personalized water-paste pills and establish a model for predicting the binder concentration and dosage. Five representative TCM materials were selected, followed by mixture uniform design. The water-paste pills were prepared by extrusion and spheronization with hypromellose E5(HPMC E5) as the binder. The quality of intermediates and final products was evaluated, and the resulting data were subjected to multivariate statistical analysis. The prediction models for binder concentration and dosage were established as follows: binder concentration: Y_1=0.378 6 + 0.570 1X_A + 2.271 2X_B-0.894 5X_C-0.458 2X_D-1.145 4X_E(when Y_1 < 0, 10% HPMC E5 was required; when Y_1 > 0, 20% HPMC E5 was required), with the accuracy reaching up to 100%; binder dosage: Y_2=32.38 + 0.25X_A + 1.85X_B-0.013X_B~2-0.002 5X_C~2(R~2=0.932 6, P < 0.001). The results showed that the binder concentration and dosage were correlated positively with the proportion of fiber material but negatively with the proportions of sugar material and brittle material. Then the validation experiments were conducted with the prediction models and all the prescriptions could be successfully prepared at one time. These demonstrated that following the classification of TCM materials and the calculation of their proportions in the prescription, the established mathematical model could be adopted for predicting the binder concentration and dosage required in the preparation of personalized water-paste pills, which contributed to reducing the pre-formulation research and guiding the actual production of personalized water-paste pills.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210422.301DOI Listing
August 2021

[Material classification of decoction pieces based on physical properties of powder].

Zhongguo Zhong Yao Za Zhi 2021 Aug;46(15):3753-3763

Health Service Collaborative Innovation Center of Shanghai Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.

Chinese medicinals feature different medicinal parts and enriched components, which makes their powders show obvious microscopic identification characteristics and specific physical properties. On this basis, the commonly used Chinese medicinals can be divided into several categories, such as powdery, fibrous, sugar, oil, and brittle materials, which is of great importance to the research and development of personalized Chinese medicinal preparation technology. However, the existing classification methods are highly subjective and thus difficult to meet the requirements for the development of personalized Chinese medicinal preparations with high quality. In this study, 55 representative Chinese medicinals, such as Dioscoreae Rhizoma and Leonuri Herba, were selected, and the physical properties of their powders were systematically characterized by comprehensive powder tester, torque rheometer, texture analyzer, etc., based on which a data set encompassing physical properties of these powders was built. The typical physical fingerprints of powders from the above 5 categories were established by multivariate statistical analysis. Then, the Chinese medicinals were classified according to the Euclidean distance between each of them and the typical value in the PCA score plot. For those with multiple material properties, whose classification boundary was fuzzy, the proportions of different types of materials were calculated with the combination of Euclidean distance, powder properties, microscopic identification characteristics, and chemical composition, so as to achieve the multivariate quantitative classification of Chinese medicinals. This lays the foundation for the further creation of intelligent personalized Chinese medicinal preparation technology.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210310.303DOI Listing
August 2021

[Characteristics and development assumptions of personalized traditional Chinese medicine granules].

Zhongguo Zhong Yao Za Zhi 2021 Aug;46(15):3746-3752

Shanghai Traditional Chinese Medicine Health Service Collaborative Innovation Center, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.

Personalized traditional Chinese medicine(TCM) granules are positioned as a solid dosage form of TCM decoctions, boasting strong applicability and wide application range. The market prospect of personalized TCM granules is promising in that their preparation by mixed decoction makes up for the shortcoming of formula granules like the Chinese patent medicine granules and classical TCM prescription granules whose components cannot be changed flexibly. However, such factors as insufficient basic research, equipment mismatch, and low process commonality have limited their clinical application. After analyzing the characteristics of perso-nalized TCM granules, their production status, and the bottlenecks restricting their development, this paper pointed out the meaning and key points of developing a generalized preparation process for personalized TCM granules and affirmed the vital roles of the preparation and process prediction system and the on-line detection technology in improving the productivity of granulation. Finally, some assumptions on technology development for solving the specific problems of personalized TCM granules were shared to provide some ideas for the application and development of personalized TCM granules in clinical practice.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210422.302DOI Listing
August 2021

[Application,problems,and development strategies of personalized traditional Chinese medicine preparations].

Zhongguo Zhong Yao Za Zhi 2021 Aug;46(15):3739-3745

Shanghai Innovation Center of Traditional Chinese Medicine Health Service, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.

With the continuous improvement of people's living standards and the greatly accelerated pace of life, patients' requirements for traditional Chinese medicine(TCM) pharmaceutical care are constantly raised, and personalized TCM preparations used are increasing year by year. However, a series of problems, such as the bottleneck of preparation technology, the lack of preparation specifications, and the weak legal basis for supervision, have become prominent, leading to the uneven product quality of personalized pre-parations. This paper systematically summarized and analyzed the current situation and existing problems of personalized TCM preparations, including dosage form application, preparation technology, production equipment, quality standard, clinical application, and administration mode. Additionally, it put forward an inheritance and innovation research mode of personalized TCM preparation technology based on the physical fingerprint of raw materials and innovation research and intellectual property protection mode of "research-patent-technology-equipment-standard". Furthermore, innovative development strategies were proposed, such as the production standard system of clinical preparations based on the whole process traceability and the regional processing service mode of clinical preparations based on industry 4.0. The present study is expected to provide beneficial references for the inheritance and innovation development of personalized TCM preparations.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210407.301DOI Listing
August 2021

Barriers and facilitators to implementing measurement-based care for depression in Shanghai, China: a situational analysis.

BMC Psychiatry 2021 09 1;21(1):430. Epub 2021 Sep 1.

Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Measurement-based care (MBC) is an evidence-based practice for depression, but its use by clinicians remains low. Enhanced MBC (eMBC), which uses digital technologies, can help to facilitate the use of MBC by clinicians and patients. Understanding factors that act as barriers and drivers to the implementation of MBC and eMBC is important to support the design of implementation strategies, promoting uptake by clinicians and patients.

Objective: This situational analysis identifies barriers and facilitators to the implementation of standard and eMBC at mental health centers in Shanghai, China.

Methods: We used mixed methods to develop a comprehensive understanding of the factors influencing MBC and eMBC implementation in Shanghai. This study took place across three mental health centers in Shanghai. We used situational analysis tools to collect contextual information about the three centers, conducted surveys with n = 116 clinicians and n = 301 patients, conducted semi-structured interviews with n = 30 clinicians and six focus groups with a total of n = 19 patients. Surveys were analysed using descriptive statistics, and semi-structured interviews and focus groups were analysed using framework analysis.

Results: Several potential barriers and facilitators to MBC and eMBC implementation were identified. Infrastructure, cost, attitudes and beliefs, and perceptions about feasibility and efficacy emerged as both challenges and drivers to MBC and eMBC implementation in Shanghai.

Conclusions: The results of this study will directly inform the design of an implementation strategy for MBC and eMBC in Shanghai, that will be tested via a randomized controlled trial. This study contributes to the emerging body of literature on MBC implementation and, to the best of our knowledge, is the first such study to take place in Asia. This study identifies several factors that are relevant to the equitable delivery of MBC, recognizing the need to explicitly address equity concerns in global mental health implementation research.
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http://dx.doi.org/10.1186/s12888-021-03442-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411506PMC
September 2021

Lensless phase retrieval based on deep learning used in holographic data storage.

Opt Lett 2021 Sep;46(17):4168-4171

This paper proposes a lensless phase retrieval method based on deep learning (DL) used in holographic data storage. By training an end-to-end convolutional neural network between the phase-encoded data pages and the corresponding near-field diffraction intensity images, the new unknown phase data page can be predicted directly from the intensity image by the network model without any iterations. The DL-based phase retrieval method has a higher storage density, lower bit-error-rate (BER), and higher data transfer rate compared to traditional iterative methods. The retrieval optical system is simple, stable, and robust to environment fluctuations which is suitable for holographic data storage. Besides, we studied and demonstrated that the DL method has a good suppression effect on the dynamic noise of the holographic data storage system.
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http://dx.doi.org/10.1364/OL.433955DOI Listing
September 2021

A Segmented Variable-Parameter ZNN for Dynamic Quadratic Minimization With Improved Convergence and Robustness.

IEEE Trans Neural Netw Learn Syst 2021 Aug 31;PP. Epub 2021 Aug 31.

As a category of the recurrent neural network (RNN), zeroing neural network (ZNN) can effectively handle time-variant optimization issues. Compared with the fixed-parameter ZNN that needs to be adjusted frequently to achieve good performance, the conventional variable-parameter ZNN (VPZNN) does not require frequent adjustment, but its variable parameter will tend to infinity as time grows. Besides, the existing noise-tolerant ZNN model is not good enough to deal with time-varying noise. Therefore, a new-type segmented VPZNN (SVPZNN) for handling the dynamic quadratic minimization issue (DQMI) is presented in this work. Unlike the previous ZNNs, the SVPZNN includes an integral term and a nonlinear activation function, in addition to two specially constructed time-varying piecewise parameters. This structure keeps the time-varying parameters stable and makes the model have strong noise tolerance capability. Besides, theoretical analysis on SVPZNN is proposed to determine the upper bound of convergence time in the absence or presence of noise interference. Numerical simulations verify that SVPZNN has shorter convergence time and better robustness than existing ZNN models when handling DQMI.
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http://dx.doi.org/10.1109/TNNLS.2021.3106640DOI Listing
August 2021

Genomic analysis uncovers prognostic and immunogenic characteristics of ferroptosis for clear cell renal cell carcinoma.

Mol Ther Nucleic Acids 2021 Sep 19;25:186-197. Epub 2021 May 19.

Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.

In this study, the characteristic patterns of ferroptosis in clear cell renal cell carcinoma (ccRCC) were systematically investigated with the interactions between ferroptosis and the tumor microenvironment (TME). On the mRNA expression profiles of 57 ferroptosis-related genes (FRGs), three ferroptosis patterns were constructed, with distinct prognosis and immune cell infiltrations (especially T cells and dendritic cells). The high ferroptosis scores were characterized by poorer prognosis, increased T cell infiltration, higher immune and stromal scores, elevated tumor mutation burden, and enhanced response to anti-CTLA4 immunotherapy. Meanwhile, the low ferroptosis scores were distinctly associated with enhanced tumor purity and amino acid and fatty acid metabolism pathways. Following validation, the ferroptosis score was an independent and effective prognostic factor. Collectively, ferroptosis could be involved in the diverse and complex TME. Evaluation of the ferroptosis patterns may heighten the comprehension about immune infiltrations in the TME, assisting oncologists to generate individualized immunotherapeutic strategies.
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http://dx.doi.org/10.1016/j.omtn.2021.05.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8368772PMC
September 2021

Advanced Metallic and Polymeric Coatings for Neural Interfacing: Structures, Properties and Tissue Responses.

Polymers (Basel) 2021 Aug 23;13(16). Epub 2021 Aug 23.

Department of Biomedical Engineering, Sun Yat-sen University, Shenzhen 518107, China.

Neural electrodes are essential for nerve signal recording, neurostimulation, neuroprosthetics and neuroregeneration, which are critical for the advancement of brain science and the establishment of the next-generation brain-electronic interface, central nerve system therapeutics and artificial intelligence. However, the existing neural electrodes suffer from drawbacks such as foreign body responses, low sensitivity and limited functionalities. In order to overcome the drawbacks, efforts have been made to create new constructions and configurations of neural electrodes from soft materials, but it is also more practical and economic to improve the functionalities of the existing neural electrodes via surface coatings. In this article, recently reported surface coatings for neural electrodes are carefully categorized and analyzed. The coatings are classified into different categories based on their chemical compositions, i.e., metals, metal oxides, carbons, conducting polymers and hydrogels. The characteristic microstructures, electrochemical properties and fabrication methods of the coatings are comprehensively presented, and their structure-property correlations are discussed. Special focus is given to the biocompatibilities of the coatings, including their foreign-body response, cell affinity, and long-term stability during implantation. This review article can provide useful and sophisticated insights into the functional design, material selection and structural configuration for the next-generation multifunctional coatings of neural electrodes.
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http://dx.doi.org/10.3390/polym13162834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401399PMC
August 2021

Identification of Solanum Immune Receptors by Bulked Segregant RNA-Seq and High-Throughput Recombinant Screening.

Methods Mol Biol 2021 ;2354:315-330

Wageningen UR Plant Breeding, Wageningen University and Research, Wageningen, The Netherlands.

The identification, understanding, and deployment of immune receptors are crucial to achieve high-level and durable resistance for crops against pathogens. In potato, many R genes have been identified using map-based cloning strategies. However, this is a challenging and laborious task that involves the development of a high number of molecular markers for the initial mapping, and the screening of thousands of plants for fine mapping. Bulked segregant RNA-Seq (BSR-Seq) has proven to be an efficient technique for the mapping of resistance genes. The RNA from two bulks of plants with contrasting phenotypes is sequenced and analyzed to identify single-nucleotide polymorphism (SNPs) markers linked to the target gene. Subsequently, the SNP markers that are identified can be used to delimit the mapping interval. Additionally, we designed an in vitro recombinant screening strategy that is advantageous for analyzing a large number of plants, in terms of time, space, and cost. Tips and detailed protocols, including BSR-Seq, bioinformatic analysis, and recombinant screening, are provided in this chapter.
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http://dx.doi.org/10.1007/978-1-0716-1609-3_15DOI Listing
January 2021

Disrupting phosphorylation of Tyr-1070 at GluN2B selectively produces resilience to depression-like behaviors.

Cell Rep 2021 Aug;36(8):109612

Department of Biophysics, Department of Neurosurgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003, P.R. China. Electronic address:

Drugs targeting N-methyl-D-aspartate receptors (NMDARs) have been approved to treat major depressive disorder (MDD); however, the presence of undesirable psychotomimetic and cognitive side effects may limit their utility. In this study, we show that the phosphorylation levels of the GluN2B subunit at tyrosine (Y) 1070 increase in mice after both acute and chronic restraint stress (CRS) exposure. Preventing GluN2B-Y1070 phosphorylation via Y1070F mutation knockin produces effects similar to those of antidepressants but does not affect cognitive or anxiety-related behaviors in subject mice. Mechanistically, the Y1070F mutation selectively reduces non-synaptic NMDAR currents and increases the number of excitatory synapses in the layer 5 pyramidal neurons of medial prefrontal cortex (mPFC) but not in the hippocampus. Altogether, our study identifies phosphorylation levels of GluN2B-Y1070 in the mPFC as a dynamic, master switch guarding depressive behaviors, suggesting that disrupting the Y1070 phosphorylation of GluN2B subunit has the potential for developing new antidepressants.
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http://dx.doi.org/10.1016/j.celrep.2021.109612DOI Listing
August 2021

Neural substrates of propranolol-induced impairments in the reconsolidation of nicotine-associated memories in smokers.

Transl Psychiatry 2021 Aug 24;11(1):441. Epub 2021 Aug 24.

Peking University Sixth Hospital, Peking University Institute of Mental Health, NHC Key Laboratory of Mental Health (Peking University), National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Chinese Academy of Medical Sciences Research Unit (No. 2018RU006), Peking University, 100191, Beijing, China.

The majority of smokers relapse even after successfully quitting because of the craving to smoking after unexpectedly re-exposed to smoking-related cues. This conditioned craving is mediated by reward memories that are frequently experienced and stubbornly resistant to treatment. Reconsolidation theory posits that well-consolidated memories are destabilized after retrieval, and this process renders memories labile and vulnerable to amnestic intervention. This study tests the retrieval reconsolidation procedure to decrease nicotine craving among people who smoke. In this study, 52 male smokers received a single dose of propranolol (n = 27) or placebo (n = 25) before the reactivation of nicotine-associated memories to impair the reconsolidation process. Craving for smoking and neural activity in response to smoking-related cues served as primary outcomes. Functional magnetic resonance imaging was performed during the memory reconsolidation process. The disruption of reconsolidation by propranolol decreased craving for smoking. Reactivity of the postcentral gyrus in response to smoking-related cues also decreased in the propranolol group after the reconsolidation manipulation. Functional connectivity between the hippocampus and striatum was higher during memory reconsolidation in the propranolol group. Furthermore, the increase in coupling between the hippocampus and striatum positively correlated with the decrease in craving after the reconsolidation manipulation in the propranolol group. Propranolol administration before memory reactivation disrupted the reconsolidation of smoking-related memories in smokers by mediating brain regions that are involved in memory and reward processing. These findings demonstrate the noradrenergic regulation of memory reconsolidation in humans and suggest that adjunct propranolol administration can facilitate the treatment of nicotine dependence. The present study was pre-registered at ClinicalTrials.gov (registration no. ChiCTR1900024412).
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http://dx.doi.org/10.1038/s41398-021-01566-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385067PMC
August 2021

ANXA1 as a Prognostic and Immune Microenvironmental Marker for Gliomas Based on Transcriptomic Analysis and Experimental Validation.

Front Cell Dev Biol 2021 4;9:659080. Epub 2021 Aug 4.

Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

The tumor microenvironment (TME) plays an important role in the growth and invasion of glioma. This study aimed to analyze the composition of the immune microenvironment in glioma samples and analyze the important differentially expressed genes to identify novel immune-targeted therapy for glioma. We downloaded transcriptomic data of 669 glioma samples from The Cancer Genome Atlas database. CIBERSORT and ESTIMATE methods were used to calculate the proportion of tumor-infiltrating immune cells and ratio of immune and stromal components in the TME. The differentially expressed genes (DEGs) were screened by comparing the genes expressed by both stromal and immune cells. Annexin A1 (ANXA1) was determined to be an important prognostic indicator through the common overlap of univariate Cox regression analysis and protein-protein interaction network analysis. The proportion of tumor-infiltrating immune cells, calculated by CIBERSORT algorithm, had a significant difference in distribution among the high and low ANXA1 expression groups, indicating that ANXA1 could be an important immune marker of TME. Furthermore, ANXA1 level was positively correlated with the histopathological factors and negatively related to the survival of glioma patients based on the analysis of multiple databases. Finally, experiments verified that antagonizing ANXA1 expression promoted cell apoptosis and inhibited the invasion and migration capacities of glioma cells. Therefore, ANXA1 due to its immune-related functions, can be an important prognostic indicator and immune microenvironmental marker for gliomas. Further studies are warranted to confirm ANXA1 as a potential immunotherapeutic target for gliomas.
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http://dx.doi.org/10.3389/fcell.2021.659080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371204PMC
August 2021

Low-Dose Radiation Can Cause Epigenetic Alterations Associated With Impairments in Both Male and Female Reproductive Cells.

Front Genet 2021 2;12:710143. Epub 2021 Aug 2.

Hong Kong Branch of the Southern Marine Science and Engineering Guangdong Laboratory, Hong Kong, China.

Humans are regularly and continuously exposed to ionizing radiation from both natural and artificial sources. Cumulating evidence shows adverse effects of ionizing radiation on both male and female reproductive systems, including reduction of testis weight and sperm count and reduction of female germ cells and premature ovarian failure. While most of the observed effects were caused by DNA damage and disturbance of DNA repairment, ionizing radiation may also alter DNA methylation, histone, and chromatin modification, leading to epigenetic changes and transgenerational effects. However, the molecular mechanisms underlying the epigenetic changes and transgenerational reproductive impairment induced by low-dose radiation remain largely unknown. In this study, two different types of human ovarian cells and two different types of testicular cells were exposed to low dose of ionizing radiation, followed by bioinformatics analysis (including gene ontology functional analysis and Ingenuity Pathway Analysis), to unravel and compare epigenetic effects and pathway changes in male and female reproductive cells induced by ionizing radiation. Our findings showed that the radiation could alter the expression of gene cluster related to DNA damage responses through the control of MYC. Furthermore, ionizing radiation could lead to gender-specific reproductive impairment through deregulation of different gene networks. More importantly, the observed epigenetic modifications induced by ionizing radiation are mediated through the alteration of chromatin remodeling and telomere function. This study, for the first time, demonstrated that ionizing radiation may alter the epigenome of germ cells, leading to transgenerational reproductive impairments, and correspondingly call for research in this new emerging area which remains almost unknown.
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http://dx.doi.org/10.3389/fgene.2021.710143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365519PMC
August 2021

Application of Da Vinci robotic surgery system in cervical cancer: A single institution experience of 557 cases.

Asian J Surg 2021 Aug 6. Epub 2021 Aug 6.

Department of Gynecology, The First Affiliated Hospital of Chongqing Medical University, China. Electronic address:

Objective: Conflicting data have been published regarding the oncologic appropriateness of minimally invasive surgery (MIS) in the treatment of cervical cancer. The purpose of the present study was to investigate whether our experience in the treatment of early cervical cancer using a robotic surgical approach was safe and oncologically effective.

Methods: The data of 557 patients with cervical cancer treated by robotic surgery were retrospectively collected, including the perioperative and survival outcomes. Tumor stage was based on the International Federation of Gynecology and Obstetrics (FIGO 2009). The disease-free survival (DFS) and overall survival (OS) were calculated by the Kaplan-Meier method.

Results: Of the 557 patients, 196 (35.2%) patients were stage IA1 to IB1, and 304 (54.6%) patients were stage IB2 to I1A2. Also included were 57 (10.2%) patients with either recurrent or persistent disease following concurrent chemoradiotherapy. Two patients (0.4%) experienced severe intraoperative complications and 11 patients (2.0%) developed postoperative complications. A stratified analysis of survival was conducted in 91 patients who met a follow-up time of 3-year or more. The median follow-up time was 49 (range, 6-57) months. Both the 3-year DFS and OS of early-stage (stage IA1 to IB1) cervical cancer were 97.6%. For patients with locally advanced (stage IB2 to IIA2) disease, DFS and OS were 88.1% and 90.5%, respectively. The patients with recurrent or persistent disease had DFS and OS of 62.5%.

Conclusion: Our study results demonstrated that the robotic surgical approach could achieve satisfying therapeutic outcomes in patients with early-stage cervical cancer, with a low complication rate. For advanced cervical cancer patients with recurrent or persistent disease following concurrent chemoradiotherapy, robotic surgery undertaken as supplementary therapy may improve prognosis. However, there remains a need for additional prospective data reporting long-term survival of cervical cancer patients treated with a robotic surgical approach.
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http://dx.doi.org/10.1016/j.asjsur.2021.07.052DOI Listing
August 2021

Characteristics of fine particulate matter (PM) at Jinsha Site Museum, Chengdu, China.

Environ Sci Pollut Res Int 2021 Aug 4. Epub 2021 Aug 4.

Chengdu Institute of Cultural Relics and Archaeology, Chengdu, 610074, China.

Air pollution is a serious threat to ancient sites and cultural relicts. In this study, we collected indoor and outdoor PM samples and individual particles at the Exhibition Hall of Jinsha Site Museum in June 2020, and then the chemical components, sources, morphology, and mixing state of the fine particulate matter were analyzed. Our results show that the indoor and outdoor PM concentrations at the Exhibition Hall were 33.3±6.6 and 39.4±11.4 μg m, respectively. Although the indoor and outdoor concentrations of OC and EC were close, the proportion of secondary organic carbon in OC outdoor (33%) was higher than that indoor (27%). The PM was alkaline both indoors and outdoors, and the outdoor alkalinity was stronger than the indoor alkalinity. SNA (SO, NO, and NH) was the dominant component in the water-soluble inorganic ions; Na, Mg, and Ca were well correlated (R> 0.9), and Cl and K were also highly correlated (R> 0.8). Enrichment factor analysis showed that Cu (indoor) and Cd were the main anthropogenic elements and that Cd was heavily enriched. Principal components analysis showed that the main sources of PM at Jinsha Site Museum were motor vehicles, dust, secondary sources, and combustion sources. The individual particles were classified as organic matter, S-rich, soot, mineral, and fly ash/metal particles, and most of these particles were internally mixed with each other. At last, we proposed pollution control measures to improve the air quality of museums and the preservation of cultural relicts.
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http://dx.doi.org/10.1007/s11356-021-15743-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336903PMC
August 2021

Fully Printed High-Performance n-Type Metal Oxide Thin-Film Transistors Utilizing Coffee-Ring Effect.

Nanomicro Lett 2021 Aug 3;13(1):164. Epub 2021 Aug 3.

Key Laboratory of 3D Micro/Nano Fabrication and Characterization of Zhejiang Province, School of Engineering, Westlake University, Hangzhou, 310024, China.

Metal oxide thin-films transistors (TFTs) produced from solution-based printing techniques can lead to large-area electronics with low cost. However, the performance of current printed devices is inferior to those from vacuum-based methods due to poor film uniformity induced by the "coffee-ring" effect. Here, we report a novel approach to print high-performance indium tin oxide (ITO)-based TFTs and logic inverters by taking advantage of such notorious effect. ITO has high electrical conductivity and is generally used as an electrode material. However, by reducing the film thickness down to nanometers scale, the carrier concentration of ITO can be effectively reduced to enable new applications as active channels in transistors. The ultrathin (~10-nm-thick) ITO film in the center of the coffee-ring worked as semiconducting channels, while the thick ITO ridges (>18-nm-thick) served as the contact electrodes. The fully inkjet-printed ITO TFTs exhibited a high saturation mobility of 34.9 cm V s and a low subthreshold swing of 105 mV dec. In addition, the devices exhibited excellent electrical stability under positive bias illumination stress (PBIS, ΔV = 0.31 V) and negative bias illuminaiton stress (NBIS, ΔV = -0.29 V) after 10,000 s voltage bias tests. More remarkably, fully printed n-type metal-oxide-semiconductor (NMOS) inverter based on ITO TFTs exhibited an extremely high gain of 181 at a low-supply voltage of 3 V, promising for advanced electronics applications.
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http://dx.doi.org/10.1007/s40820-021-00694-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8333237PMC
August 2021

Artesunate relieves acute kidney injury through inhibiting macrophagic Mincle-mediated necroptosis and inflammation to tubular epithelial cell.

J Cell Mol Med 2021 Sep 1;25(18):8775-8788. Epub 2021 Aug 1.

Research Center of Integrated Traditional Chinese and Western Medicine, Affiliated Traditional Medicine Hospital, Southwest Medical University, Luzhou, China.

Artesunate is a widely used derivative of artemisinin for malaria. Recent researches have shown that artesunate has a significant anti-inflammatory effect on many diseases. However, its effect on acute kidney injury with a significant inflammatory response is not clear. In this study, we established a cisplatin-induced AKI mouse model and a co-culture system of BMDM and tubular epithelial cells (mTEC) to verify the renoprotective and anti-inflammatory effects of artesunate on AKI, and explored the underlying mechanism. We found that artesunate strongly down-regulated the serum creatinine and BUN levels in AKI mice, reduced the necroptosis of tubular cells and down-regulated the expression of the tubular injury molecule Tim-1. On the other hand, artesunate strongly inhibited the mRNA expression of inflammatory cytokines (IL-1β, IL-6 and TNF-α), protein levels of inflammatory signals (iNOS and NF-κB) and necroptosis signals (RIPK1, RIPK3 and MLKL) in kidney of AKI mouse. Notably, the co-culture system proved that Mincle in macrophage can aggravate the inflammation and necroptosis of mTEC induced by LPS, and artesunate suppressed the expression of Mincle in macrophage of kidney in AKI mouse. Overexpression of Mincle in BMDM restored the damage and necroptosis inhibited by artesunate in mTEC, indicating Mincle in macrophage is the target of artesunate to protect tubule cells in AKI. Our findings demonstrated that artesunate can significantly improve renal function in AKI, which may be related to the inhibition of Mincle-mediated macrophage inflammation, thereby reducing the damage and necroptosis to tubular cells that provide new option for the treatment of AKI.
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http://dx.doi.org/10.1111/jcmm.16833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435453PMC
September 2021

Artesunate relieves acute kidney injury through inhibiting macrophagic Mincle-mediated necroptosis and inflammation to tubular epithelial cell.

J Cell Mol Med 2021 Sep 1;25(18):8775-8788. Epub 2021 Aug 1.

Research Center of Integrated Traditional Chinese and Western Medicine, Affiliated Traditional Medicine Hospital, Southwest Medical University, Luzhou, China.

Artesunate is a widely used derivative of artemisinin for malaria. Recent researches have shown that artesunate has a significant anti-inflammatory effect on many diseases. However, its effect on acute kidney injury with a significant inflammatory response is not clear. In this study, we established a cisplatin-induced AKI mouse model and a co-culture system of BMDM and tubular epithelial cells (mTEC) to verify the renoprotective and anti-inflammatory effects of artesunate on AKI, and explored the underlying mechanism. We found that artesunate strongly down-regulated the serum creatinine and BUN levels in AKI mice, reduced the necroptosis of tubular cells and down-regulated the expression of the tubular injury molecule Tim-1. On the other hand, artesunate strongly inhibited the mRNA expression of inflammatory cytokines (IL-1β, IL-6 and TNF-α), protein levels of inflammatory signals (iNOS and NF-κB) and necroptosis signals (RIPK1, RIPK3 and MLKL) in kidney of AKI mouse. Notably, the co-culture system proved that Mincle in macrophage can aggravate the inflammation and necroptosis of mTEC induced by LPS, and artesunate suppressed the expression of Mincle in macrophage of kidney in AKI mouse. Overexpression of Mincle in BMDM restored the damage and necroptosis inhibited by artesunate in mTEC, indicating Mincle in macrophage is the target of artesunate to protect tubule cells in AKI. Our findings demonstrated that artesunate can significantly improve renal function in AKI, which may be related to the inhibition of Mincle-mediated macrophage inflammation, thereby reducing the damage and necroptosis to tubular cells that provide new option for the treatment of AKI.
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http://dx.doi.org/10.1111/jcmm.16833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435453PMC
September 2021

The effect of health literacy on patient's perceived shared decision-making among Chinese cancer patients.

Psychooncology 2021 Jul 29. Epub 2021 Jul 29.

School of Nursing, Southern Medical University, Guangzhou, China.

Objectives: To explore the relationship between patient's health literacy and perceived shared decision-making (SDM) among Chinese cancer patients.

Methods: A cross-sectional study was conducted involving a convenience sample of 458 cancer patients from four public hospitals in Guangzhou, China. Patients' self-reported data were collected using the Health Literacy Management Scale (HeLMS) and the nine-item Shared Decision-Making Questionnaire (SDM-Q-9). Hierarchical multiple regressions, controlling for patient-doctor relationship, social support, sociodemographic and clinical variables were conducted to explore the effect of health literacy on perceived SDM.

Results: Health literacy itself accounted for 68.0% of the variance in perceived SDM. Higher scores in domains "information acquisition ability," and "communication interaction ability" of HeLMS were significantly associated with a higher level of perceived SDM after controlling the covariates (R  = 75.7%).

Conclusions: Health literacy, especially the information acquisition ability and communication interaction ability, played a prominent role for Chinese cancer patients to be involved in treatment decision making.
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http://dx.doi.org/10.1002/pon.5777DOI Listing
July 2021

A New Strategy to Quantitatively Identify Hot-Spot Areas in Growth of New HIV Infections for Targeted Interventions.

Front Public Health 2021 12;9:680867. Epub 2021 Jul 12.

National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Previous geographic studies of HIV infection have usually used prevalence data, which cannot indicate the hot-spot areas of current transmission. To develop quantitative analytic measures for accurately identifying hot-spot areas in growth of new HIV infection, we investigated the geographic distribution features of recent HIV infection and long-term HIV infection using data from a whole-population physical examination in four key counties in Liangshan prefecture, which are most severely affected by HIV in China. Through a whole-population physical examination during November 2017- June 2018 in the four key counties, a total of 5,555 HIV cases were diagnosed and 246 cases were classified as recently infected by laboratory HIV recency tests. The geospatial patterns of recent and long-term HIV infected cases were compared using ordinary least squares regression and Geodetector. Further, geospatial-heterogeneity was quantified and indicated using a residual map to visualize hot-spot areas where new infection is increasing. The geographic location of HIV cases showed an uneven distribution along major roads and clustered at road intersections. The geographic mapping showed that several areas were clustered with more recently infected HIV cases than long-term infected cases. The quantitative analyses showed that the geospatial asymmetry between recent and long-term HIV infection was 0.30 and 0.31 in ordinary least squares regression and Geodetector analysis, respectively. The quantitative analyses found twenty-three townships showing an increase in the number of recent infections. Quantitative analysis of geospatial-heterogeneous areas by comparing between recent and long-term HIV infections allows accurate identification of hot-spot areas where new infections are expanding, which can be used as a potent methodological tool to guide targeted interventions and curb the spread of the epidemic.
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http://dx.doi.org/10.3389/fpubh.2021.680867DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310914PMC
August 2021

Information, deliberation, and decisional control preferences for participation in medical decision-making and its influencing factors among Chinese cancer patients.

Health Expect 2021 Jul 26. Epub 2021 Jul 26.

Evidence-based Nursing and Midwifery Practice Center, School of Nursing, Southern Medical University, Guangzhou, China.

Background: Patient-centred care should strive to respond to the individual patient's needs and preferences when possible. Yet, preferences of cancer patients for participation in different stages of the medical decision-making process to increase matching of preferred and actual participation of the patients in decision-making are not well known.

Objective: This study aimed to determine the level of information, deliberation and decisional control preferences in decision-making practices among Chinese cancer patients and to explore their association with the sociodemographic and clinical variables of the patients.

Methods: A cross-sectional study was conducted involving a convenience sample of 328 cancer patients from three public hospitals in Guangzhou, China. The Patient Expectation for Participation in Medical Decision-making Scale (PEPMDS) was used to evaluate information, deliberation and decisional control preferences of the patients. Binary logistic regressions were conducted to determine the association between sociodemographic variables, clinical variables and preferences of the patients.

Results: Most patients reported a high preference for information (73.2%) and deliberation (73.8%), while a small number (37.2%) reported a high preference for decisional control. Younger patients and patients with higher levels of education were significantly more likely to have a high preference for information, deliberation and decisional control. Patients with low annual family incomes were significantly more likely to have a low preference for decisional control.

Conclusion: Preferences of patients for involvement in different stages of decision-making practices could vary. The level of preferences appears to be related to the patient's age, education level, and financial status.

Practice Implications: Healthcare providers should tailor the participatory approaches of patients considering individual preferences for information, deliberation and decisional control during medical decision-making.

Patient Contribution: Patients participated in the survey and filled in the questionnaires.
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http://dx.doi.org/10.1111/hex.13312DOI Listing
July 2021
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