Publications by authors named "Lin Wang"

5,912 Publications

  • Page 1 of 1

Association Between White Matter Hyperintensities and Chronic Kidney Disease: A Systematic Review and Meta-Analysis.

Front Med (Lausanne) 2022 3;9:770184. Epub 2022 May 3.

Department of Neurology, The Affiliated Jiangning Hospital With Nanjing Medical University, Nanjing, China.

Objectives: Previous studies of the associations between white matter hyperintensities (WMH) and chronic kidney disease (CKD) were still conflicting; therefore, our study aimed to conduct a systematic review of all of the available research on this topic and a meta-analysis of the association between WMH and CKD among observational studies.

Setting And Design: Systematic review and meta-analysis.

Outcome Measures: Severity of WMH.

Methods And Participants: All relevant studies in public databases were examined until 15 November 2020. Two independent reviewers assessed all the included studies using the Cross-Sectional/Prevalence Study Quality (CSSQ) scale, and then literature review and meta-analyses were undertaken.

Results: We pooled the odds ratio (OR) for the presence of WMH, periventricular hyperintensities (PVH), and deep subcortical white matter hyperintensities (DWMH) of patients with CKD vs. non-CKD patients by subgroup analysis, and the results obtained were WMH OR 2.07, 95% CI [1.58, 2.70], PVH OR 2.41, 95% CI [1.90, 3.05], and DWMH OR 2.11, 95% CI [1.60, 2.80], respectively. The main outcome showed that patients with CKD were more likely to have WMH in the brain compared to the normal controls. Another meta-analysis showed a statistically significant decline in renal function in patients with moderate to severe WMH compared with those with no to mild WMH.

Conclusions: The findings indicated that patients with CKD were more likely to experience WMH than demographically matched controls. On the other hand, patients with moderate to severe WMH in the brain had poor renal function more frequently than those with no to mild WMH.
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http://dx.doi.org/10.3389/fmed.2022.770184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112853PMC
May 2022

Performance Evaluation of Knowledge Sharing in an Industry-University-Research Alliance Based on PSO-BPNN.

Authors:
Lin Wang

Comput Intell Neurosci 2022 10;2022:1283588. Epub 2022 May 10.

Graduate School, Jilin Jianzhu University, Changchun, China.

Knowledge sharing performance is very important to evaluate the interests of industry university research alliance. Firstly, this paper puts forward the index system of knowledge sharing performance evaluation of industry university research alliance. A BP neural network (BPNN) and a PSO-improved BP neural network (PSO-BPNN) are used to establish the evaluation model, and the accuracy of the model is evaluated. The prediction accuracy is evaluated by comparing the predicted value with the target value. Through comparative analysis, it is found that the performance evaluation model based on PSO-BP neural network has high accuracy and applicability, and is an effective method of alliance knowledge sharing performance evaluation.
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http://dx.doi.org/10.1155/2022/1283588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113886PMC
May 2022

Ultrathin two-dimensional hybrid perovskites toward flexible electronics and optoelectronics.

Natl Sci Rev 2022 May 19;9(5):nwab129. Epub 2021 Jul 19.

Key Laboratory of Flexible Electronics and Institute of Advanced Materials, School of Physical and Mathematical Sciences, Nanjing Tech University, China.

Ultrathin hybrid perovskites combine the advantages of 2D morphology and organic-inorganic components. This perspective article provides an updated summary and new insights for their development in flexible electronics and optoelectronics.
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http://dx.doi.org/10.1093/nsr/nwab129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113132PMC
May 2022

Effects of Bacterial Culture and Calcium Source Addition on Lead and Copper Remediation Using Bioinspired Calcium Carbonate Precipitation.

Front Bioeng Biotechnol 2022 2;10:889717. Epub 2022 May 2.

School of Civil Engineering, Xi'an University of Architecture and Technology, Xi'an, China.

Lead and copper ions from wastewater induced by metallurgical processes are accumulated in soils, threatening plant and human health. The bioinspired calcium carbonate precipitation is proven effective in improving the cementation between soil particles. However, studies on capsulizing heavy metal ions using the bioinspired calcium carbonate precipitation are remarkably limited. The present study conducted a series of test tube experiments to investigate the effects of bacterial culture and calcium source addition on the remediation efficiency against lead and copper ions. The calcium carbonate precipitation was reproduced using the Visual MINTEQ software package to reveal the mechanism affecting the remediation efficiency. The degradation in the remediation efficiency against lead ions relies mainly upon the degree of urea hydrolysis. However, higher degrees of urea hydrolysis cause remediation efficiency against copper ions to reduce to zero. Such high degree of urea hydrolysis turns pH surrounding conditions into highly alkaline environments. Therefore, pursuing higher degrees of urea hydrolysis might not be the most crucial factor while remedying copper ions. The findings shed light on the importance of modifying pH surrounding conditions in capsulizing copper ions using the bioinspired calcium carbonate precipitation.
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http://dx.doi.org/10.3389/fbioe.2022.889717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108487PMC
May 2022

Synthesis and Antitumor Activity of Diosgenin Hydroxamic Acid and Quaternary Phosphonium Salt Derivatives.

ACS Med Chem Lett 2022 May 19;13(5):786-791. Epub 2022 Apr 19.

Key Laboratory of General Chemistry of the National Ethnic Affairs Commission, School of Chemistry and Environment, Southwest Minzu University, Chengdu 610041, China.

Diosgenin, a component separated from Dioscorea plants, is an important starting material for steroid hormone drugs and semisynthetic steroids. In the work, two series of diosgenin derivatives were designed, synthesized, and evaluated for their cellular anticancer activities. Most of the target compounds exhibited good inhibitory activities against four cell lines, Aspc-1 (human colon adenocarcinoma cells), H358 (human nonsmall cell lung cancer cells), HCT116 (human colorectal adenocarcinoma cells), and SW620 (human metastatic pancreatic cancer cells). Among them, the representative compound exhibited 7.9-341.7-fold antiproliferative activities against the above-mentioned four cell lines compared with the lead compound diosgenin.
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http://dx.doi.org/10.1021/acsmedchemlett.1c00581DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109269PMC
May 2022

SIK2 Improving Mitochondrial Autophagy Restriction Induced by Cerebral Ischemia-Reperfusion in Rats.

Front Pharmacol 2022 2;13:683898. Epub 2022 May 2.

Department of Ultrasonic Medicine, Yijishan Hospital, Wannan Medical College, Wuhu, China.

Previous studies have shown that Salt-induced kinase-2(SIK2) is involved in the regulation of various energy-metabolism-related reactions, and it also can regulate angiogenesis after cerebral ischemia-reperfusion. However, it is unclear whether SIK2 can regulate energy metabolism in cerebral ischemia-reperfusion injury. As mitochondria plays an important role in energy metabolism, whether SIK2 regulates energy metabolism through affecting mitochondrial changes is also worth to be explored. In this study, rats were treated with adeno-associated virus-SIK2-Green fluorescent protein (AAV-SIK2-GFP) for the overexpression of SIK2 before middle cerebral artery occlusion (MCAO). We found that SIK2 overexpression could alleviate the neuronal damage, reduce the area of cerebral infarction, and increase the adenosine triphosphate (ATP) content, which could promote the expression of phosphorylated-mammalian target of rapamycin-1 (p-mTORC1), hypoxia-inducible factor-1α (HIF-1α), phosphatase and tensin homologue-induced putative kinase 1 (PINK1) and E3 ubiquitinligating enzyme (Parkin). Transmission electron microscopy revealed that SIK2 overexpression enhanced mitochondrial autophagy. It is concluded that SIK2 can ameliorate neuronal injury and promote the energy metabolism by regulating the mTOR pathway during cerebral ischemia-reperfusion, and this process is related to mitochondrial autophagy.
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http://dx.doi.org/10.3389/fphar.2022.683898DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108450PMC
May 2022

A Comparative Study of Total Knee Arthroplasty and Unicondylar Knee Arthroplasty in the Treatment of Knee Osteoarthritis.

Contrast Media Mol Imaging 2022 28;2022:7795801. Epub 2022 Apr 28.

Department of Nuclear Medicine, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou 310014, Zhejiang, China.

Objective: To compare the clinical efficacy of total knee arthroplasty (TKA) and unicondylar knee arthroplasty (UKA) in the treatment of knee osteoarthritis (KOA).

Methods: A retrospective analysis was conducted on 30 patients admitted to the Department of Orthopaedics of Yijishan Hospital from 2020 to 2021. The patients were divided into UKA group ( = 15) and TKA group (control,  = 15). The intraoperative situation and postoperative clinical indicators of patients in the two groups were collected and compared, such as operation time, intraoperative blood loss, length of hospital stay, postoperative complications, and postoperative functional recovery. Postoperative functional recovery was investigated by the visual analogue pain scale (VAS), knee score scale (HSS), and knee range of motion (ROM) scores 5 days after surgery.

Results: Perioperative indexes in the UKA group were significantly lower than those in the TKA group, including operation time, intraoperative blood loss, first time going to the ground, and length of hospital stay. VAS, HSS, and ROM scores in the two groups were significantly improved after surgery compared with those before surgery. However, ROM scores in the UKA group were significantly better than in the TKA group. In terms of early postoperative complications, there was one case of venous thrombosis of lower limbs in the UKA group, while in the TKA group there was one case of delayed wound healing due to diabetes, and one case of deep infection.

Conclusion: Both UKA and TKA are very successful options for the treatment of KOA, but the use of UKA can promote the recovery of postoperative knee function, reduce postoperative complications, and achieve more satisfactory than expected results.
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http://dx.doi.org/10.1155/2022/7795801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071932PMC
May 2022

ELONGATED HYPOCOTYL 5-mediated Suppression of Melatonin Biosynthesis is Alleviated by Dark and Promotes Cotyledon Opening.

J Exp Bot 2022 May 17. Epub 2022 May 17.

College of Horticulture, China Agricultural University, Beijing, China.

Melatonin (N-acetyl-5-methoxytryptamine) biosynthesis in plants is induced by darkness and high-intensity light; however, the underlying transcriptional mechanisms and upstream signaling pathway are unknown. We identified a dark-induced and highly-expressed melatonin synthetase in Arabidopsis thaliana, AtSNAT6, encoding serotonin N-acetyltransferase. We assessed melatonin and AtSNAT6 expression levels in mutants lacking key regulators of light/dark signaling. AtCOP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1)/AtHY5 (ELONGATED HYPOCOTYL 5), which controls light/dark transition and photomorphogenesis, promoted/suppressed melatonin biosynthesis. Using EMSA and ChIP-qPCR analysis, we showed that AtHY5 inhibits AtSNAT6 expression directly. An analysis of melatonin levels in snat6 hy5 and AtHY5+AtSNAT6-overexpressing plants confirmed their regulatory function in melatonin biosynthesis. Exogenous melatonin further inhibited cotyledon opening in hy5 and AtSNAT6-overexpressing seedlings, but partially reversed the promotion of cotyledon opening in AtHY5-overexpressing seedlings and snat6. Additionally, the CRISPR/Cas9-mediated mutation of AtSNAT6 increased cotyledon opening in hy5, while overexpressing AtSNAT6 decreased it in AtHY5-overexpressing seedlings via changing melatonin biosynthesis, confirming that AtHY5 decreased the melatonin-mediated inhibition of cotyledon opening. It provides new insights into the regulation of melatonin biosynthesis and the roles of melatonin in cotyledon opening.
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http://dx.doi.org/10.1093/jxb/erac176DOI Listing
May 2022

Abnormal brain functional and structural connectivity between the left supplementary motor area and inferior frontal gyrus in moyamoya disease.

BMC Neurol 2022 May 16;22(1):179. Epub 2022 May 16.

Department of Neurosurgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Jiefang Road 88th, Hangzhou, 310009, China.

Background: Disruption of brain functional connectivity has been detected after stroke, but whether it also occurs in moyamoya disease (MMD) is unknown. Impaired functional connectivity is always correlated with abnormal white matter fibers. Herein, we used multimodal imaging techniques to explore the changes in brain functional and structural connectivity in MMD patients.

Methods: We collected structural images, resting-state functional magnetic resonance imaging (rs-fMRI) and diffusion tensor imaging for each subject. Cognitive functions of MMD patients were evaluated using the Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), and Trail Making Test parts A and B (TMT-A/-B). We calculated the functional connectivity for every paired region using 90 regions of interest from the Anatomical Automatic Labeling Atlas and then determined the differences between MMD patients and HCs. We extracted the functional connectivity of paired brain regions with significant differences between the two groups. Correlation analyses were then performed between the functional connectivity and variable cognitive functions. To explore whether the impaired functional connectivity and cognitive performances were attributed to the destruction of white matter fibers, we further analyzed fiber integrity using tractography between paired regions that were correlated with cognition.

Results: There was lower functional connectivity in MMD patients as compared to HCs between the bilateral inferior frontal gyrus, between the bilateral supramarginal gyrus, between the left supplementary motor area (SMA) and the left orbital part of the inferior frontal gyrus (IFGorb), and between the left SMA and the left middle temporal gyrus (P < 0.01, FDR corrected). The decreased functional connectivity between the left SMA and the left IFGorb was significantly correlated with the MMSE (r = 0.52, P = 0.024), MoCA (r = 0.60, P = 0.006), and TMT-B (r = -0.54, P = 0.048) in MMD patients. White matter fibers were also injured between the SMA and IFGorb in the left hemisphere and were positively correlated with reduced functional connectivity.

Conclusions: Brain functional and structural connectivity between the supplementary motor area and inferior frontal gyrus in the left hemisphere are damaged in MMD. These findings could be useful in the evaluation of disease progression and prognosis of MMD.
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http://dx.doi.org/10.1186/s12883-022-02705-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108139PMC
May 2022

Biomimetic modification on the microporous surface of cardiovascular materials to accelerate endothelialization and regulate intimal regeneration.

Mater Sci Eng C Mater Biol Appl 2022 Jan 19:112666. Epub 2022 Jan 19.

Medical College of Acu-Moxi and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China. Electronic address:

Vascular stents are widely used in the clinical treatment of coronary heart disease, but the long-term safety still needs to be improved. Surface biological functional modification is an effective way to improve the biocompatibility and clinical performance of cardiovascular materials, but how to achieve long-term effective and precise regulation of in situ vascular intimal repair through the reasonable construction of the surface physical and chemical structure is still an important task in the current surface modification research. In this study, ECM-derived components, including laminin, heparin, and SDF-1, were incorporated into the titanium surface with a microporous structure. It was found that the modified surface could effectively control the continuous release of biomolecules. In vitro biocompatibility evaluation results showed that the constructed functional layer could effectively inhibit the activation of platelet adhesion and excessive proliferation of smooth muscle cells. In addition, the modified surface also showed the potential to induce rapid regeneration of vascular endothelium. In vivo animal tests further proved that the modified sample may contribute to inhibiting vascular intimal hyperplasia. This study provided a new approach for the surface biological function modification of Ti-based vascular stents.
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http://dx.doi.org/10.1016/j.msec.2022.112666DOI Listing
January 2022

Design, synthesis, and biological evaluation of novel pyrazolo [3,4-d]pyrimidine derivatives as potent PLK4 inhibitors for the treatment of TRIM37-amplified breast cancer.

Eur J Med Chem 2022 May 10;238:114424. Epub 2022 May 10.

Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang, 110016, PR China.

Serine/threonine-protein kinase polo-like kinase 4 (PLK4) is a mitosis-associated protein kinase that plays a vital role in the duplication of centrioles in dividing cells and is considered a promising target of synthetic lethality in TRIM37-amplified breast cancer. Herein, based on a rational drug design strategy, we described a series of pyrazolo [3,4-d]pyrimidine derivatives as potent PLK4 inhibitors and dissected the relevant structure-activity relationships (SARs). Most compounds showed potent suppressive activities against PLK4, with IC values of < 10 nM. Among them, compound 24j (PLK4 IC = 0.2 nM) displayed potent enzyme inhibition and good selectivity in a panel of 35 kinases. At the cellular level, compound 24j exhibited notable antiproliferative activities against MCF-7, BT474, and MDA-MB-231 cells, with IC values of 0.36, 1.35, and 2.88 μM, respectively. Compound 24j killed TRIM37-amplified breast cancer cells. Moreover, we evaluated the clone formation, proliferation, cycle arrest, and migration abilities of compound 24j using MCF-7 cells. Furthermore, the in vitro preliminary evaluation of the drug-like properties of compound 24j showed remarkable plasma stability, moderate liver microsomal stability, and weak inhibitory activity against the main subtypes of human cytochrome P450. Based on in vivo pharmacokinetic studies in Sprague Dawley rats, compound 24j exhibited a relatively high plasma clearance and a low F value (8.03%). Overall, these results support the further development of compound 24j as a potential lead compound to treat TRIM37-amplified breast cancer.
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http://dx.doi.org/10.1016/j.ejmech.2022.114424DOI Listing
May 2022

Mixed-Dimensional MXene-Based Composite Electrodes Enable Mechanically Stable and Efficient Flexible Perovskite Light-Emitting Diodes.

Nano Lett 2022 May 16. Epub 2022 May 16.

Key Laboratory of Advanced Display and System Applications of Ministry of Education, Shanghai University, 149 Yanchang Road, Shanghai 200072, People's Republic of China.

Significant advancements in perovskite light-emitting diodes (PeLEDs) based on ITO glass substrates have been realized in recent years, yet the overall performance of flexible devices still lags far behind, mainly being ascribed to the high surface roughness and poor optoelectronic properties of flexible electrodes. Here, we report efficient and robust flexible PeLEDs based on a mixed-dimensional (0D-1D-2D-3D) composite electrode consisting of 0D Ag nanoparticles (AgNPs)/1D Ag nanowires (AgNWs)/2D MXene/3D poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS). Our designed MXene-based electrodes combine the advantages of facile formation of a film of low-dimensional materials and excellent optical and electrical properties of metal, inorganic, and organic semiconductors, which endow the electrodes with high electrical/thermal conductivity, flexibility, a smooth surface, and good transmittance. Consequently, the resulting flexible PeLEDs (without a light-coupling structure) demonstrate a record external quantum efficiency of 16.5%, a high luminance of close to 50000 cd/m, a large emitting area of 8 cm, and significantly enhanced mechanical stability.
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http://dx.doi.org/10.1021/acs.nanolett.2c01517DOI Listing
May 2022

Imaging Features Derived From Dynamic Contrast-Enhanced Magnetic Resonance Imaging to Differentiate Malignant From Benign Breast Lesions: A Systematic Review and Meta-Analysis.

J Comput Assist Tomogr 2022 May-Jun 01;46(3):383-391

Department of Radiology, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.

Materials And Methods: The PubMed, Web of Science, Embase, and the Cochrane Library databases were searched up to January 2021 to identify original studies that investigated the accuracy of individual DCE-MRI features in differentiating malignant from benign breast lesions. Pooled sensitivity, specificity, and area under the curve were calculated by STATA software based on the data extracted from included studies. Moreover, quality assessment, subgroup analysis, and publication bias evaluation were performed.

Results: Twenty-nine studies comprising 2976 patients and 3365 suspicious breast lesions were included. Malignant breast lesions tended to present irregular shapes (83.59%), noncircumscribed margins (85.50%), mass enhancement (52.31%), heterogeneous internal enhancement (71.72%), and type II or III time intensity curve (TIC) patterns (91.17%), showing significant differences compared with benign breast lesions (P < 0.05). For differentiating malignant from benign breast lesions, the area under the curve values of irregular shape, noncircumscribed margin, mass enhancement, heterogeneous internal enhancement, and type II or III TIC patterns were 0.79 (0.76-0.83), 0.87 (0.84-0.90), 0.63 (0.58-0.67), 0.82 (0.78-0.85), and 0.89 (0.86-0.92), respectively.

Conclusions: Imaging features derived from DCE-MRI, especially TIC patterns, are important for diagnosing and differentiating malignant from benign breast lesions.
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http://dx.doi.org/10.1097/RCT.0000000000001289DOI Listing
May 2022

Trichosanthin inhibits cell growth and metastasis by promoting pyroptosis in non-small cell lung cancer.

J Thorac Dis 2022 Apr;14(4):1193-1202

Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, China.

Background: A number of studies have demonstrated that trichosanthin (TCS) can induce apoptosis in numerous types of tumor cell lines. However, whether TCS can induce pyroptosis has not yet been reported. This study aimed to investigate the role of TCS and its inhibitory effect on tumor growth by modulating pyroptosis in non-small cell lung cancer (NSCLC).

Methods: Effects of different concentrations of TCS on the cell viability, proliferation, migration and invasion of NSCLC were detected by Cell Counting Kit-8 (CCK-8), colony formation, migration, and invasion assays. Immunofluorescence was used to detect the effect of TCS on the expression of pyroptosis marker protein gasdermin-D (GSDMD)-N in A549 cells. A tumor xenograft animal model was established by injecting A549 cells into nude mice.

Results: In the present study, we found that TCS significantly inhibited the proliferation, migration, and invasion of A549 cells in a concentration-dependent manner. In addition, TCS at a high concentration (40 µg/mL) significantly promoted the expression of pyroptosis-related proteins [GSDMD-N, NLR family pyrin domain containing 3 (NLRP3), apoptosis-associated speck-like protein containing a CARD (ASC), caspase-1, and GSDMD], which showed an inhibitory effect on the pyroptosis of A549 cells. Additionally, we found that necrosulfonamide (NSA) significantly reversed the inhibitory effect of high concentrations of TCS on the pyroptosis of A549 cells. The in vivo experiments showed that TCS effectively reduced the tumor volume and inhibited the expression of Ki-67, whereas it increased the expression of GSDMD-N.

Conclusions: Taken together, these results indicated that TCS could inhibit the progression of NSCLC by promoting pyroptosis. These findings provide further information on the possible underlying mechanism of TCS in the treatment of NSCLC.
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http://dx.doi.org/10.21037/jtd-22-282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096284PMC
April 2022

Effects of Site-Directed Mutagenesis of Cysteine on the Structure of Sip Proteins.

Front Microbiol 2022 29;13:805325. Epub 2022 Apr 29.

College of Life Science, Northeast Agricultural University, Harbin, China.

, a gram-positive bacteria, has three insecticidal proteins: Vip (vegetative insecticidal protein), Cry (crystal), and Sip (secreted insecticidal protein). Of the three, Sip proteins have insecticidal activity against larvae of Coleoptera. However, the Sip1Aa protein has little solubility in the supernatant because of inclusion bodies. This makes it more difficult to study, and thus research on Sip proteins is limited, which hinders the study of their mechanistic functions and insecticidal mechanisms. This highlights the importance of further investigation of the Sip1Aa protein. Disulfide bonds play an important role in the stability and function of proteins. Here, we successfully constructed mutant proteins with high insecticidal activity. The tertiary structure of the Sip1Aa protein was analyzed with homologous modeling and bioinformatics to predict the conserved domain of the protein. Cysteine was used to replace amino acids via site-directed mutagenesis. We successfully constructed Sip149-251, Sip153-248, Sip158-243, and Sip178-314 mutant proteins with higher solubility than Sip1Aa. Sip153-248 and Sip158-243 were the most stable compared to Sip1Aa, followed by Sip149-251 and Sip178-314. The insecticidal activity of Sip153-248 (Sip158-243) was 2.76 (2.26) times higher than that of Sip1Aa. The insecticidal activity of Sip149-251 and Sip178-314 did not differ significantly from that of Sip1Aa. Basic structural properties, physicochemical properties, and the spatial structure of the mutation site of Sip1Aa and the mutant proteins were analyzed. These results provide a molecular basis for using Sip1Aa to control Coleopteran insects and contribute to the study of the Sip1Aa insecticidal mechanism.
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http://dx.doi.org/10.3389/fmicb.2022.805325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100928PMC
April 2022

Effects of the Intermittent Theta Burst Stimulation of the Cerebellar Vermis on Balance Recovery After Stroke: A Study Protocol for a Randomized Controlled Trial.

Front Aging Neurosci 2022 29;14:881311. Epub 2022 Apr 29.

Department of Rehabilitation, Wuxi Tongren Rehabilitation Hospital, Wuxi, China.

Background: The recovery of balance function is a critical segment in the rehabilitation treatment of stroke. The cerebellum is considered as the key structure involved in balance and motor control. The cerebellar vermis plays an important role in integrating vision, proprioception, and sensory skin input and may be a candidate stimulation target for regulating the motor network related with balance. However, evidence that the intermittent theta burst stimulation (iTBS) of cerebellar vermis can promote the recovery of balance function after stroke remains insufficient. Therefore, this study aims to explore the efficacy of the cerebellar vermis iTBS for the treatment of balance function in patients with stroke.

Methods And Analysis: Forty patients with stroke will be recruited in this prospective, randomized, sham-controlled trial. Participants will be randomized in a 1:1 ratio to receive either 15 sessions of cerebellar vermis iTBS (600 pulses) or sham stimulation. Additionally, a routine rehabilitation therapy follows the intervention. The primary outcome is the Berg Balance Scale, and the secondary outcomes are the Fugl-Meyer assessment of the lower extremity and modified Barthel index. The above outcomes will be assessed before intervention and at the end of each week. Pre- and post-iTBS resting-state functional magnetic resonance imaging (rs-fMRI) will be acquired, and the regional homogeneity, fractional amplitude of low-frequency fluctuation and functional connectivity will be calculated and analyzed.

Discussion: This protocol holds promise as a potential method to improve balance function in patients with stroke. If the outcomes of patients improve after the intervention, the study will provide new insights into improving balance function.

Ethics And Dissemination: This study has been approved by the Medical Research Ethics Committee of Wuxi Mental Health Center (Wuxi Tongren Rehabilitation Hospital). Results will be disseminated through (open-access) peer-reviewed publications, networks of scientists, professionals, and the public and presented at conferences.

Clinical Trial Registration Number: www.chictr.org.cn, identifier ChiCTR2100052590.
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http://dx.doi.org/10.3389/fnagi.2022.881311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099377PMC
April 2022

Research on the Design of Assistant Basketball Teaching System Based on Big Data.

Comput Intell Neurosci 2022 6;2022:1499555. Epub 2022 May 6.

College of Physical Education, Hengshui University, Hebei, China.

Due to the limitations of the current teaching resources searched by the system, it is difficult to achieve good results in basketball teaching. For this reason, an auxiliary basketball teaching system based on big data is designed. On the basis of clarifying the basic principles of teaching system design, JSP (Java Server Pages) dynamic web page back-end technology and Tomcat 7.0 server are used as the system support structure. Take the big data environment as the source of teaching resources and match them to different databases according to the characteristics of the resources. And combined with real-time evaluation to achieve the assessment of teaching effects and the management of teaching resources, the test results show that the average score of the basketball technical action test under the guidance of this system is higher than 7.5 points when the experimental subjects have the same level of basketball skills. In the paired sample -test and the independent sample -test of the performance level test scores =0.001 < 0.05, there is a significant difference. It shows that the system can effectively improve the teaching effect of basketball and improve students' basketball test scores.
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http://dx.doi.org/10.1155/2022/1499555DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106470PMC
May 2022

The lip gene contributes to the virulence of Aeromonas veronii strain TH0426.

Microb Pathog 2022 May 12;167:105566. Epub 2022 May 12.

College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China. Electronic address:

Aeromonas veronii (A. veronii) is a pathogen that can infect aquatic organisms and mammals and has caused irrecoverable economic losses to the aquaculture industry. The results of an epidemiological investigation showed that the number of cases of A. veronii have increased gradually in recent years, and its drug resistance and virulence has shown an upward trend. In this study, we constructed an A. veronii mutant strain Δlip, by homologous recombination and studied its function. The results showed that there was no significant difference in the biofilm formation ability between the Δlip and the wild-type strain, but the toxicity of the Δlip to EPC cells and its ability to adhere to EPC cells were significantly reduced. The LD value of the Δlip to zebrafish was 7.40-fold higher than that of the wild-type strain. In addition, after 24 h and 72 h, the bacterial loads of the Δlip in the organs of crucian carp were significantly lower than those in the wild-type strain. In conclusion, the mutant strain Δlip led to a decrease in the adhesion and virulence of the wild-type strain, which lays a foundation to further understand lip gene function and the pathogenic mechanism of A. veronii.
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http://dx.doi.org/10.1016/j.micpath.2022.105566DOI Listing
May 2022

Genomic architecture of fetal central nervous system anomalies using whole-genome sequencing.

NPJ Genom Med 2022 May 13;7(1):31. Epub 2022 May 13.

Maternal and Child Health Hospital of Hubei Province, Hubei, 430070, China.

Structural anomalies of the central nervous system (CNS) are one of the most common fetal anomalies found during prenatal imaging. However, the genomic architecture of prenatal imaging phenotypes has not yet been systematically studied in a large cohort. Patients diagnosed with fetal CNS anomalies were identified from medical records and images. Fetal samples were subjected to low-pass and deep whole-genome sequencing (WGS) for aneuploid, copy number variation (CNV), single-nucleotide variant (SNV, including insertions/deletions (indels)), and small CNV identification. The clinical significance of variants was interpreted based on a candidate gene list constructed from ultrasound phenotypes. In total, 162 fetuses with 11 common CNS anomalies were enrolled in this study. Primary diagnosis was achieved in 62 cases, with an overall diagnostic rate of 38.3%. Causative variants included 18 aneuploids, 17 CNVs, three small CNVs, and 24 SNVs. Among the 24 SNVs, 15 were novel mutations not reported previously. Furthermore, 29 key genes of diagnostic variants and critical genes of pathogenic CNVs were identified, including five recurrent genes: i.e., TUBA1A, KAT6B, CC2D2A, PDHA1, and NF1. Diagnostic variants were present in 34 (70.8%) out of 48 fetuses with both CNS and non-CNS malformations, and in 28 (24.6%) out of 114 fetuses with CNS anomalies only. Hypoplasia of the cerebellum (including the cerebellar vermis) and holoprosencephaly had the highest primary diagnosis yields (>70%), while only four (11.8%) out of 34 neural tube defects achieved genetic diagnosis. Compared with the control group, rare singleton loss-of-function variants (SLoFVs) were significantly accumulated in the patient cohort.
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http://dx.doi.org/10.1038/s41525-022-00301-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106651PMC
May 2022

Accurate observation of black and brown carbon in atmospheric fine particles via a versatile aerosol concentration enrichment system (VACES).

Sci Total Environ 2022 May 10:155817. Epub 2022 May 10.

Department of Atmospheric and Oceanic Sciences, Institute of Atmospheric Sciences, Fudan University, Shanghai 200438, China; Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention, National Observations and Research Station for Wetland Ecosystems of the Yangtze Estuary, Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China; IRDR International Center of Excellence on Risk Interconnectivity and Governance on Weather/Climate Extremes Impact and Public Health, Institute of Atmospheric Sciences, Fudan University, Shanghai 200438, China. Electronic address:

Carbonaceous aerosols (CAs) are major components of fine particulate matter (PM) that dramatically influence the energy budget of Earth. However, accurate assessment of the climatic impacts of CAs is still challenging due to the large uncertainties remaining in the measurement of their optical properties. In this respect, a modified versatile aerosol concentration enrichment system integrated into optical instruments (VACES-OPTS) was set up to increase particle concentration and amplify signal-noise ratio during optical measurement. Based on the novel technique, this study was able to lower the detection limit of CAs by an order of magnitude under high temporal resolution (2 h) and small sampling flow (6 L min). Besides, stable and reliable optical data were obtained for absorption apportionment and source identification of black carbon (BC) and brown carbon (BrC). In the field application of the new system, high absorption coefficient of CAs in Shanghai, China was witnessed. Further analysis of the contribution of black carbon BC and BrC to light absorption revealed that BrC could account for over 15% of the total absorption at 370 nm. According to the potential source contribution function model (PSCF) classification, CAs with strong light absorption in urban Shanghai originated not only from highly polluted inland China but also from active marine ship emissions.
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http://dx.doi.org/10.1016/j.scitotenv.2022.155817DOI Listing
May 2022

Accurate observation of black and brown carbon in atmospheric fine particles via a versatile aerosol concentration enrichment system (VACES).

Sci Total Environ 2022 May 10:155817. Epub 2022 May 10.

Department of Atmospheric and Oceanic Sciences, Institute of Atmospheric Sciences, Fudan University, Shanghai 200438, China; Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention, National Observations and Research Station for Wetland Ecosystems of the Yangtze Estuary, Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China; IRDR International Center of Excellence on Risk Interconnectivity and Governance on Weather/Climate Extremes Impact and Public Health, Institute of Atmospheric Sciences, Fudan University, Shanghai 200438, China. Electronic address:

Carbonaceous aerosols (CAs) are major components of fine particulate matter (PM) that dramatically influence the energy budget of Earth. However, accurate assessment of the climatic impacts of CAs is still challenging due to the large uncertainties remaining in the measurement of their optical properties. In this respect, a modified versatile aerosol concentration enrichment system integrated into optical instruments (VACES-OPTS) was set up to increase particle concentration and amplify signal-noise ratio during optical measurement. Based on the novel technique, this study was able to lower the detection limit of CAs by an order of magnitude under high temporal resolution (2 h) and small sampling flow (6 L min). Besides, stable and reliable optical data were obtained for absorption apportionment and source identification of black carbon (BC) and brown carbon (BrC). In the field application of the new system, high absorption coefficient of CAs in Shanghai, China was witnessed. Further analysis of the contribution of black carbon BC and BrC to light absorption revealed that BrC could account for over 15% of the total absorption at 370 nm. According to the potential source contribution function model (PSCF) classification, CAs with strong light absorption in urban Shanghai originated not only from highly polluted inland China but also from active marine ship emissions.
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http://dx.doi.org/10.1016/j.scitotenv.2022.155817DOI Listing
May 2022

MicroRNAs as T Lymphocyte Regulators in Multiple Sclerosis.

Front Mol Neurosci 2022 25;15:865529. Epub 2022 Apr 25.

Department of Emergency Medicine, Shengjing Hospital of China Medical University, Shenyang, China.

MicroRNA (miRNA) is a class of endogenous non-coding small RNA with regulatory activities, which generally regulates the expression of target genes at the post-transcriptional level. Multiple Sclerosis (MS) is thought to be an autoimmune-mediated chronic inflammatory demyelinating disease of the central nervous system (CNS) that typically affect young adults. T lymphocytes play an important role in the pathogenesis of MS, and studies have suggested that miRNAs are involved in regulating the proliferation, differentiation, and functional maintenance of T lymphocytes in MS. Dysregulated expression of miRNAs may lead to the differentiation balance and dysfunction of T lymphocytes, and they are thus involved in the occurrence and development of MS. In addition, some specific miRNAs, such as miR-155 and miR-326, may have potential diagnostic values for MS or be useful for discriminating subtypes of MS. Moreover, miRNAs may be a promising therapeutic strategy for MS by regulating T lymphocyte function. By summarizing the recent literature, we reviewed the involvement of T lymphocytes in the pathogenesis of MS, the role of miRNAs in the pathogenesis and disease progression of MS by regulating T lymphocytes, the possibility of differentially expressed miRNAs to function as biomarkers for MS diagnosis, and the therapeutic potential of miRNAs in MS by regulating T lymphocytes.
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http://dx.doi.org/10.3389/fnmol.2022.865529DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082748PMC
April 2022

Clinical value and expression of Homer 1, homocysteine, S-adenosyl-l-homocysteine, fibroblast growth factors 23 in coronary heart disease.

BMC Cardiovasc Disord 2022 May 12;22(1):215. Epub 2022 May 12.

Department of Cardiology, Renmin Hospital, Hubei University of Medicine, No. 39 Chaoyang Middle Road, Shiyan, 442000, Hubei, China.

Background: This study aimed to explore clinical value and expression of Homer 1, S-adenosyl-l-homocysteine (SAH), homocysteine (Hcy), fibroblast growth factors (FGF) 23 in coronary heart disease (CHD).

Methods: From March 2020 to April 2021, a total of 137 patients with CHD and 138 healthy subjects who came to our hospital for physical examination and had no cardiovascular disease were retrospectively enrolled, and they were assigned to the CHD group and the control group, respectively. Patients in the CHD group were divided into stable angina pectoris (SAP) group (n = 48), unstable angina pectoris (UAP) group (n = 46), and acute myocardial infarction (AMI) group (n = 43) according to clinical characteristics for subgroup analysis. The degree of coronary artery stenosis was assessed by Gensini score, which is a reliable assessment tool for the severity of coronary artery disease. The levels of Homer 1, SAH, Hcy, and FGF 23 were tested and compared. Spearman correlation analysis was used to analyze the correlation between serum Homer1, SAH, Hcy, FGF23 levels and Gensini score, and multivariate unconditional Logistic regression was used to analyze the risk factors of coronary heart disease.

Results: Demographic characteristics of each group were comparable (P > 0.05). The body mass index (BMI), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglyceride (TG), and glucose levels of the SAP group, UAP group and AMI group were significantly higher than those of the control group, and the number of patients with smoking, alcohol consumption, hypertension, and diabetes history was significantly more than that of the control group, respectively (P < 0.05). The level of high-density lipoprotein cholesterol (HDL-C) of each subgroup was significantly lower than the control group (P < 0.05). The above indicators showed no significant difference among three subgroups (P > 0.05). Serum SAH, Hcy, Homer1 and FGF23 levels in each subgroup were significantly higher than those in control group (P < 0.05). And above indicators in SAP group and UAP group were significantly lower than those in AMI group (P < 0.05), and the levels of above indicators in SAP group were significantly lower than those in UAP group (P < 0.05). The results of Spearman correlation analysis showed that serum Homer1, FGF23, SAH, Hcy levels were positively correlated with Gensini score (r = 0.376, 0.623, 0.291, 0.372, all P < 0.01). Multivariate logistic regression analysis showed that smoking, hypertension, diabetes, alcohol consumption, obesity, HDL-C, FGF23, SAH, Hcy, Homer 1 were independent risk factors for coronary heart disease.

Conclusion: The levels of FGF23, SAH, Hcy, and Homer1 tend to increase in patients with CHD compared with normal population, and the more severe the disease, the higher the levels, which has certain reference value for the clinical diagnosis of CHD and the evaluation and monitoring of the disease.
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http://dx.doi.org/10.1186/s12872-022-02554-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097103PMC
May 2022

A glucose-like metabolite deficient in diabetes inhibits cellular entry of SARS-CoV-2.

Nat Metab 2022 May 9. Epub 2022 May 9.

Institute of Pathogenic Organisms, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.

The severity and mortality of COVID-19 are associated with pre-existing medical comorbidities such as diabetes mellitus. However, the underlying causes for increased susceptibility to viral infection in patients with diabetes is not fully understood. Here we identify several small-molecule metabolites from human blood with effective antiviral activity against SARS-CoV-2, one of which, 1,5-anhydro-D-glucitol (1,5-AG), is associated with diabetes mellitus. The serum 1,5-AG level is significantly lower in patients with diabetes. In vitro, the level of SARS-CoV-2 replication is higher in the presence of serum from patients with diabetes than from healthy individuals and this is counteracted by supplementation of 1,5-AG to the serum from patients. Diabetic (db/db) mice undergo SARS-CoV-2 infection accompanied by much higher viral loads and more severe respiratory tissue damage when compared to wild-type mice. Sustained supplementation of 1,5-AG in diabetic mice reduces SARS-CoV-2 loads and disease severity to similar levels in nondiabetic mice. Mechanistically, 1,5-AG directly binds the S2 subunit of the SARS-CoV-2 spike protein, thereby interrupting spike-mediated virus-host membrane fusion. Our results reveal a mechanism that contributes to COVID-19 pathogenesis in the diabetic population and suggest that 1,5-AG supplementation may be beneficial to diabetic patients against severe COVID-19.
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http://dx.doi.org/10.1038/s42255-022-00567-zDOI Listing
May 2022

High-fructose corn syrup promotes proinflammatory Macrophage activation via ROS-mediated NF-κB signaling and exacerbates colitis in mice.

Int Immunopharmacol 2022 May 6;109:108814. Epub 2022 May 6.

Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Electronic address:

The dramatically increasing incidence and prevalence of inflammatory bowel disease (IBD) are reportedly related to a Western diet, which is characterized by high sugar consumption. Dietary simple sugars aggravate IBD in animal models. However, the mechanisms underlying this effect remain unclear. Given that high-fructose corn syrup (HFCS) is a major added sugar in food and beverages, we focus on HFCS and investigated the effects of HFCS on a dextran sulfate sodium (DSS)-induced murine colitis model and in RAW264.7 macrophages. Our data demonstrate that short-term consumption of HFCS aggravates colitis and upregulates the proportion of macrophages in IBD mice but not in healthy mice. We find that HFCS promotes proinflammatory cytokine production through reactive oxygen species (ROS)-mediated nuclear factor-κB (NF-κB) signaling in RAW264.7 macrophages. Furthermore, N-acetylcysteine (NAC), an ROS scavenger, alleviates HFCS-aggravated colitis in mice and inhibits the ROS-mediated NF-κB signaling pathway in RAW264.7 macrophages. Our work unveils the important role of macrophages in HFCS-induced exacerbation of colitis and reveals the mechanism of how HFCS immunologically aggravates IBD.
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http://dx.doi.org/10.1016/j.intimp.2022.108814DOI Listing
May 2022

Assessing the role of multiple mechanisms increasing the age of dengue cases in Thailand.

Proc Natl Acad Sci U S A 2022 May 9;119(20):e2115790119. Epub 2022 May 9.

Department of Biology, University of Florida, Gainesville, FL 32611.

SignificanceThe age of reported dengue hemorrhagic fever (DHF) cases, the severe form of dengue infections, has been increasing in Thailand for four decades. Factors underlying this shift remain poorly understood, challenging public health planning. Here, we found aging of the population and its effect on the hazard of transmission to be the dominant contributors, with temporal changes in surveillance practices playing a lesser role. With ongoing population aging, we expect a continuing shift of DHF toward older individuals, heightening the chance of clinical complications with comorbidities. With most other highly endemic countries facing similar shifts in age structure, the pattern is expected to appear elsewhere. Awareness is needed to improve diagnosis and treatment.
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http://dx.doi.org/10.1073/pnas.2115790119DOI Listing
May 2022

Nonmonotonic wavelength dependence of the polarization-sensitive infrared photoresponse of an anisotropic semimetal.

Nanoscale 2022 May 19;14(19):7314-7321. Epub 2022 May 19.

State Key Laboratory of Infrared Physics, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China.

Layered semimetals with in-plane anisotropy are promising for advanced polarization-sensitive infrared detection. The investigation of the polarization-dependent photoresponse of semimetals over the whole visible-to-long-wave-infrared range and revealing the physical connection between their optoelectronic properties, optical properties, and electronic band structures is required, but there have been very few studies of this kind. In this work, we conducted a thorough investigation on the polarization-dependent infrared photoresponse of WTe over the visible-to-long-wave-infrared range and discovered a textbook-like perfect consistency between the wavelength-dependent polarization-sensitive photoresponse and the anisotropic dielectric constant mainly affected by interband transitions near the Weyl point. It is revealed that the polarization sensitivity and the responsivity both vary non-monotonically with the wavelength. This phenomenon is attributed to the polarization selective excitation of interband transitions associated with asymmetrically distributed electron orbitals around the Weyl points. Concerning the infrared detection properties of WTe, a maximum responsivity of 0.68 mA W is obtained under self-powered operation. The power dependence of the photoresponse is linear, and the response time is around 14 μs. This work would provoke further studies about the anisotropic photoresponse associated with the transitions even closer to the Dirac or Weyl points, and it provides an approach to select the right semimetal for the right wavelength range of infrared polarization detection.
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http://dx.doi.org/10.1039/d1nr08268jDOI Listing
May 2022

Pulsed electromagnetic fields attenuate glucocorticoid-induced bone loss by targeting senescent LepR bone marrow mesenchymal stromal cells.

Mater Sci Eng C Mater Biol Appl 2021 Dec 28:112635. Epub 2021 Dec 28.

Deepartment of Rehabilitation Medicine, Key Laboratory of Rehabilitation Medicine, Institute of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China. Electronic address:

Glucocorticoids induce cellular senescence, including of stem/progenitor cells in the bone marrow of growing long bone, which leads to bone loss in glucocorticoid-induced osteoporosis. There is no specific drug available to treat this disease. Clearance of senescent cells has been reported to be an effective therapy for osteoporosis. LepR cells in the bone marrow are the key kind of stem/progenitor cells conducive to bone remodeling in adulthood. Here, we show that glucocorticoids induce cellular senescence, demonstrated by increased activity of senescence-associated β-galactosidase (SA-βGal) cells and simultaneous upregulation of coimmunofluorescence staining of p16 with LepR in longitudinal femoral sections. We applied pulsed electromagnetic fields (PEMFs), which alleviated bone loss in glucocorticoid-induced osteoporosis mice. We found the increased accumulation of senescent LepR cells in the bone marrow of adult mice after glucocorticoid treatment, and this could be counteracted by PEMFs. Moreover, PEMFs maintained type H vessel formation and osteogenesis. This process was modulated by the polycomb histone methyltransferase enhancer of zeste homolog 2 (EZH2) and its trimethylation of histone H3 on lysine 27 (H3K27me3). These results provide evidence that PEMFs alleviate bone loss induced by glucocorticoids by eliminating senescent cells, maintaining angiogenesis-osteogenesis coupling, and shedding light on the mechanisms, providing a potential method to treat glucocorticoid-induced osteoporosis.
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http://dx.doi.org/10.1016/j.msec.2021.112635DOI Listing
December 2021

Hepatic MDM2 Causes Metabolic Associated Fatty Liver Disease by Blocking Triglyceride-VLDL Secretion via ApoB Degradation.

Adv Sci (Weinh) 2022 May 7:e2200742. Epub 2022 May 7.

Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.

Dysfunctional triglyceride-very low-density lipoprotein (TG-VLDL) metabolism is linked to metabolic-associated fatty liver disease (MAFLD); however, the underlying cause remains unclear. The study shows that hepatic E3 ubiquitin ligase murine double minute 2 (MDM2) controls MAFLD by blocking TG-VLDL secretion. A remarkable upregulation of MDM2 is observed in the livers of human and mouse models with different levels of severity of MAFLD. Hepatocyte-specific deletion of MDM2 protects against high-fat high-cholesterol diet-induced hepatic steatosis and inflammation, accompanied by a significant elevation in TG-VLDL secretion. As an E3 ubiquitin ligase, MDM2 targets apolipoprotein B (ApoB) for proteasomal degradation through direct protein-protein interaction, which leads to reduced TG-VLDL secretion in hepatocytes. Pharmacological blockage of the MDM2-ApoB interaction alleviates dietary-induced hepatic steatohepatitis and fibrosis by inducing hepatic ApoB expression and subsequent TG-VLDL secretion. The effect of MDM2 on VLDL metabolism is p53-independent. Collectively, these findings suggest that MDM2 acts as a negative regulator of hepatic ApoB levels and TG-VLDL secretion in MAFLD. Inhibition of the MDM2-ApoB interaction may represent a potential therapeutic approach for MAFLD treatment.
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http://dx.doi.org/10.1002/advs.202200742DOI Listing
May 2022

Paediatric traumatic cataracts in Southwest China: epidemiological profile.

BMC Ophthalmol 2022 May 6;22(1):208. Epub 2022 May 6.

Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu, 61000, Sichuan, China.

Background: Paediatric traumatic cataracts are an important but preventable cause of acquired blindness. Understanding the epidemiology of paediatric traumatic cataracts is a prerequisite for prevention. This study aimed to characterize the epidemiological profile of paediatric traumatic cataracts in southwest China.

Methods: The medical records of children (age range, 0-14 years old) who developed traumatic cataracts following open-globe injuries and were hospitalized at the Department of Ophthalmology at West China Hospital, between January 2011 and December 2020 were retrospectively analyzed. The demographic data, causes of injuries, posttraumatic complications, and visual acuity were recorded and analysed.

Results: A total of 716 eyes from 716 patients were analysed in this study, including 521 (72.8%) males and 195 females in a gender ratio of 2.67:1; 117 of the patients were of ethnic minorities. Paediatric traumatic cataracts occurred more frequently in winter (32.5%). Sharp metal objects (scissors/knives/needles/sheet metal/nails/darts) - induced ocular injuries accounted for the highest proportion, followed by botanical sticks (wooden sticks /bamboo sticks /bamboo skewers)-induced injuries, and then stationery items (pencils/pens/rulers/paper)-induced injuries. The majority (68.7%) of the patients were aged 2-8 years, and the peak range of age was 4 - 6 years. The injuries were a result of penetrating trauma in 64.9% of patients, and blunt force trauma in the remainder (35.1%). Additionally, 131 (18.3%) cases developed posttraumatic infectious endophthalmitis after injuries. Patients with eye injuries caused by needles (P < 0.001), wooden sticks (P = 0.016), and bamboo skewers (P = 0.002) were at a greater risk of developing infectious endophthalmitis. The most common identified foreign organism was Streptococcus, which accounted for 42% (21/50) of all culture-positive specimens and was sensitive to vancomycin. Among the children who were younger than 5 years, 44.4% (55/124) of those with traumatic cataracts presented a corrected distance visual acuity less than or equal to 0.1 after undergoing cataract surgery, but among the children who were older than 5 years, this proportion was significantly smaller, just 20.4% of children aged 6-10 years (P < 0.001) and 18.4% of children aged 11-14 years (P < 0.001).

Conclusion: The main causative agents of paediatric traumatic cataracts in southwest China were sharp metal objects, botanical sticks, and stationery items. Specific preventive measures are essential to reduce the incidence of paediatric traumatic cataract.
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http://dx.doi.org/10.1186/s12886-022-02435-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075711PMC
May 2022
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