Publications by authors named "Lin Qiu"

358 Publications

Environmental eustress modulates β-ARs/CCL2 axis to induce anti-tumor immunity and sensitize immunotherapy against liver cancer in mice.

Nat Commun 2021 09 30;12(1):5725. Epub 2021 Sep 30.

State Key Laboratory of Oncogenes and Related Genes, Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.

Although psycho-social stress is a well-known factor that contributes to the development of cancer, it remains largely unclear whether and how environmental eustress influences malignant diseases and regulates cancer-related therapeutic responses. Using an established eustress model, we demonstrate that mice living in an enriched environment (EE) are protected from carcinogen-induced liver neoplasia and transplantable syngeneic liver tumors, owning to a CD8 T cell-dependent tumor control. We identify a peripheral Neuro-Endocrine-Immune pathway in eustress, including Sympathetic nervous system (SNS)/β-adrenergic receptors (β-ARs)/CCL2 that relieves tumor immunosuppression and overcomes PD-L1 resistance to immunotherapy. Notably, EE activates peripheral SNS and β-ARs signaling in tumor cells and tumor infiltrated myeloid cells, leading to suppression of CCL2 expression and activation of anti-tumor immunity. Either blockade of CCL2/CCR2 or β-AR signaling in EE mice lose the tumor protection capability. Our study reveales that environmental eustress via EE stimulates anti-tumor immunity, resulting in more efficient tumor control and a better outcome of immunotherapy.
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http://dx.doi.org/10.1038/s41467-021-25967-9DOI Listing
September 2021

WISER Survivor Trial: Combined Effect of Exercise and Weight Loss Interventions on Insulin and Insulin Resistance in Breast Cancer Survivors.

Nutrients 2021 Sep 4;13(9). Epub 2021 Sep 4.

Department of Public Health Sciences, College of Medicine, Pennsylvania State University, Hershey, PA 17033, USA.

Obesity-associated breast cancer recurrence is mechanistically linked with elevated insulin levels and insulin resistance. Exercise and weight loss are associated with decreased breast cancer recurrence, which may be mediated through reduced insulin levels and improved insulin sensitivity. This is a secondary analysis of the WISER Survivor clinical trial examining the relative effect of exercise, weight loss and combined exercise and weight loss interventions on insulin and insulin resistance. The weight loss and combined intervention groups showed significant reductions in levels of: insulin, C-peptide, homeostatic model assessment 2 (HOMA2) insulin resistance (IR), and HOMA2 beta-cell function (β) compared to the control group. Independent of intervention group, weight loss of ≥10% was associated with decreased levels of insulin, C-peptide, and HOMA2-IR compared to 0-5% weight loss. Further, the combination of exercise and weight loss was particularly important for breast cancer survivors with clinically abnormal levels of C-peptide.
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http://dx.doi.org/10.3390/nu13093108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469124PMC
September 2021

Bifunctional Smart Hydrogel Dressing with Strain Sensitivity and NIR-Responsive Performance.

ACS Appl Mater Interfaces 2021 Oct 24;13(39):46938-46950. Epub 2021 Sep 24.

Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.

Smart response hydrogel has a broad application prospect in human health real-time monitoring due to its responses to a variety of stimuli. In this study, we developed a novel smart hydrogel dressing based on conductive MXene nanosheets and a temperature-sensitive PNIPAm polymer. γ-Methacryloxypropyltrimethoxysilane (KH570) was selected to functionalize the surface of MXene further to improve the interface compatibility between MXene and PNIPAm. Our prepared K-M/PNIPAm hydrogel was found to have a strain-sensitive property, as well as a respond to NIR phase change and volume change. When applied as a strain flexible sensor, this K-M/PNIPAm hydrogel exhibited a high strain sensitivity with a gauge factor (GF) of 4.491, a broad working strain range of ≈250%, a fast response of ∼160 ms, and good cycle stability (i.e., 3000 s at 20% strain). Besides, this K-M/PNIPAm hydrogel can be used as an efficient NIR light-controlled drug release carrier to achieve on-demand drug release. This work paved the way for the application of smart response hydrogel in human health real-time monitoring and NIR-controlled drug release functions.
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http://dx.doi.org/10.1021/acsami.1c15312DOI Listing
October 2021

and Investigation of S1PR1 Expression in the Central Nervous System Using [H]CS1P1 and [C]CS1P1.

ACS Chem Neurosci 2021 Oct 13;12(19):3733-3744. Epub 2021 Sep 13.

Department of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, United States.

Sphingosine-1-phosphate receptor 1 (S1PR1) is ubiquitously expressed among all tissues and plays key roles in many physiological and cellular processes. In the central nervous system (CNS), S1PR1 is expressed in different types of cells including neurons, astrocytes, and oligodendrocyte precursor cells. S1PR1 has been recognized as a novel therapeutic target in multiple sclerosis and other diseases. We previously reported a promising S1PR1-specific radioligand, [C]CS1P1 (previously named [C]TZ3321), which is under clinical investigation for human use. In the current study, we performed a detailed characterization of [H]CS1P1 for its binding specificity to S1PR1 in CNS using autoradiography and immunohistochemistry in human and rat CNS tissues. Our data indicate that [H]CS1P1 binds to S1PR1 in human frontal cortex tissue with a of 3.98 nM and a of 172.5 nM. The distribution of [H]CS1P1 in human and rat CNS tissues is consistent with the distribution of S1PR1 detected by immunohistochemistry studies. Our microPET studies of [C]CS1P1 in a nonhuman primate (NHP) show a standardized uptake value of 2.4 in the NHP brain, with test-retest variability of 0.23% among six different NHPs. Radiometabolite analysis in the plasma samples of NHP and rat, as well as in rat brain samples, showed that [C]CS1P1 was stable . Kinetic modeling studies using a two-compartment tissue model showed that the positron emission tomography (PET) data fit the model well. Overall, our study provides a detailed characterization of [H]CS1P1 binding to S1PR1 in the CNS. Combined with our microPET studies in the NHP brain, our data suggest that [C]CS1P1 is a promising radioligand for PET imaging of S1PR1 in the CNS.
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http://dx.doi.org/10.1021/acschemneuro.1c00492DOI Listing
October 2021

Overuse wrist injuries in adolescent platform and springboard divers.

Res Sports Med 2021 Aug 20:1-12. Epub 2021 Aug 20.

Department of Radiology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, China.

To investigate overuse wrist injuries in adolescent divers using magnetic resonance imaging (MRI). The bilateral wrists (76 wrists) of 38 professional divers were examined by MRI; 42 wrists with pain were placed in the symptomatic group, and 34 wrists without pain were placed in the asymptomatic group. Two experienced radiologists assessed the wrist injuries. Chi‑square test was used to compare the difference in injuries between the two groups. Subchondral osteosclerosis was observed in 47.62% of symptomatic wrists and 52.94% of asymptomatic wrists. Distal radial epiphyseal plate injury was detected in 42.86% of symptomatic wrists and 11.76% of asymptomatic wrists. Triangular fibrocartilage complex injury was observed in 19.04% of symptomatic wrists and 8.82% of asymptomatic wrists. Bursae around the wrist were observed in 21.42% of symptomatic wrists and 17.64% of asymptomatic wrists. The incidence of distal radial epiphyseal plate injury was higher in the symptomatic group than in the asymptomatic group ( = 0.007), however, other injuries were not statistically different between the two groups ( > 0.05). This study demonstrated that adolescent divers had various wrist injuries, and distal radial epiphyseal plate injury was more common in the symptomatic wrist; these injuries show no specific clinical symptoms and are easily overlooked.
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http://dx.doi.org/10.1080/15438627.2021.1966009DOI Listing
August 2021

The potential utility of [ Ga]Ga-DOTA-FAPI-04 as a novel broad-spectrum oncological and non-oncological imaging agent-comparison with [F]FDG.

Eur J Nucl Med Mol Imaging 2021 Aug 19. Epub 2021 Aug 19.

Department of Nuclear Medicine, The Affiliated Hospital of Southwest Medical University, No. 25 Taiping St, Jiangyang District, Luzhou, 646000, Sichuan, People's Republic of China.

Purpose: This study aimed to compare the detection performance of [ Ga]Ga-DOTA-FAPI-04 and [F]FDG PET/CT in the patients with various oncological and non-oncological lesions.

Methods: A total of 123 patients underwent contemporaneous [ Ga]Ga-DOTA-FAPI-04 and [F]FDG PET/CT were included in this prospective study. The maximum standard uptake value (SUVmax) was measured to compare oncological and non-oncological lesion uptake. The sensitivity, specificity, predictive values, and accuracy of [F]FDG and [ Ga]Ga-DOTA-FAPI-04 PET/CT for detecting primary, metastatic, and non-oncological lesions were calculated and compared to evaluate the detection efficacy.

Results: The study subjects consisted of 123 patients (69 men and 54 women; mean age 56.11 ± 11.94 years). Among the 102 patients with either newly diagnosed (82 patients) or previously treated solid tumor (20 patients), a total of 88 solid primary malignant tumors in 84/102 patients were detected. Two patients had two primary tumors each and 1 patient had three primary tumors. Among them, 58/102 and 43/102 patients had nodal (376 lesions) and distant metastases (406 lesions), respectively. Eight patients had hematological neoplasm. No malignant oncological diseases were detected in the remaining 13 patients. A total of 145 non-oncological lesions and benign tumors in 52/123 patients were detected incidentally. [ Ga]Ga-DOTA-FAPI-04 PET/CT demonstrated a significantly higher uptake and detection rate for the primary (SUVmax 10.98 ± 5.83 vs. 8.36 ± 6.43, p < 0.001; sensitivity 97.67 vs. 84.89%; and accuracy 96.59 vs. 82.95%, X = 0.538, p = 0.021), nodal (SUVmax 10.50 ± 5.98 vs. 8.20 ± 6.29, p = 0.011; sensitivity 97.59 vs. 84.72%; and accuracy 97.34 vs. 84.31%, X = 2.067, p < 0.001), and distant metastatic lesions (SUVmax 9.64 ± 6.45 vs. 6.74 ± 4.83; p < 0.001; sensitivity 98.01 vs. 65.59%; and accuracy 97.04 vs. 65.51%, X = 4.897, p < 0.001) of solid tumor than did [F]FDG PET/CT. [ Ga]Ga-DOTA-FAPI-04 PET/CT demonstrated a lower activity (SUVmax: 6.84 ± 4.67 vs. 13.09 ± 7.29, p < 0.001) and detection rate (sensitivity 50.65 vs. 96.75%, and accuracy 51.28 vs. 95.51%, X = 5.166, p < 0.001) for multiple myeloma and lymphoma compared to [F]FDG PET/CT. [ Ga]Ga-DOTA-FAPI-04 and [F]FDG PET/CT PET/CT demonstrated a comparative activity (SUVmax 6.40 ± 3.95 vs. 5.74 ± 15.78, p = 0.729) and detection efficacy (sensitivity 86.52 vs. 72.34%, and accuracy 84.83 vs. 72.41%, X = 9.460, p = 0.007) for non-oncological lesion and benign tumor detection.

Conclusions: Except for myeloma and lymphoma, [ Ga]Ga-DOTA-FAPI-04 PET/CT showed a superior detection efficacy for detecting various primary and metastatic lesions than [F]FDG PET/CT. A comparative detection utility for non-oncological lesion was obtained with both tracers. [ Ga]Ga-DOTA-FAPI-04 could be used as a broad-spectrum tumor and inflammatory imaging agent in the clinical especially for various solid tumors and non-oncological lesions.
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http://dx.doi.org/10.1007/s00259-021-05522-wDOI Listing
August 2021

68Ga-FAPI PET/CT Detected Non-FDG-Avid Gastric Stromal Tumor.

Clin Nucl Med 2021 Aug 5. Epub 2021 Aug 5.

From the Department of Nuclear Medicine, The Affiliated Hospital of Southwest Medical University Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province Academician (Expert) Workstation of Sichuan Province Department of Radiology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, People's Republic of China.

Abstract: We presented a case of 67-year-old man with epigastric discomfort and anorexia for more than 2 months. No abnormal 18F-FDG uptake was found throughout the gastrointestinal tract on 18F-FDG PET/CT. The patient was enrolled in the 68Ga-FAPI PET/CT clinical trial, and a 68Ga-FAPI PET/CT was performed to help detect primary lesion. This examination demonstrated a nodular thickening of the gastric wall with an increased 68Ga-FAPI uptake in the greater curvature of the stomach. Finally, the pathological result confirmed the diagnosis of gastric stromal tumor. This case highlights that 68Ga-FAPI PET/CT might outperform 18F-FDG PET/CT in helping identify gastrointestinal stromal tumors.
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http://dx.doi.org/10.1097/RLU.0000000000003856DOI Listing
August 2021

Silica-Coated FeO Nanoparticles as a Bifunctional Agent for Magnetic Resonance Imaging and ZnII Fluorescent Sensing.

Technol Cancer Res Treat 2021 Jan-Dec;20:15330338211036539

School of Pharmacy & School of Medicine, 12412Changzhou University, Changzhou, People's Republic of China.

Bifunctional magnetic/fluorescent core-shell silica nanospheres (MNPs) encapsulated with the magnetic FeO core and a derivate of 8-amimoquinoline (N-(quinolin-8-yl)-2-(3-(triethoxysilyl) propylamino) acetamide) (QTEPA) into the shell were synthesized. These functional MNPs were prepared with a modified stöber method and the formed [email protected] core-shell nanocomposites are biocompatible, water-dispersible, and stable. These prepared nanoparticles were characterized by X-ray power diffraction (XRD), transmission electron microscopy (TEM), thermoelectric plasma Quad II inductively coupled plasma mass spectrometry (ICP-MS), superconducting quantum interference device (SQUID), TG/DTA thermal analyzer (TGA) and Fourier transform infrared spectroscopy (FTIR). Further application of the nanoparticles in detecting Zn was confirmed by the fluorescence experiment: the nanosensor shows high selectivity and sensitivity to Zn with a 22-fold fluorescence emission enhancement in the presence of 10 μM Zn. Moreover, the transverse relaxivity measurements show that the core-shell MNPs have T2 relaxivity (r2) of 155.05 mM S based on Fe concentration on the 3.0 T scanner, suggesting that the compound can be used as a negative contrast agent for MRI. Further experiments showed that these MNPs could be used as MRI contrast agent. Therefore, the new nanosensor provides the dual modality of magnetic resonance imaging and optical imaging.
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http://dx.doi.org/10.1177/15330338211036539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358497PMC
August 2021

Modulation of Aggregation-Caused Quenching to Aggregation-Induced Emission: Finding a Biocompatible Polymeric Theranostics Platform for Cancer Therapy.

Macromol Rapid Commun 2021 Oct 12;42(19):e2100264. Epub 2021 Aug 12.

School of Pharmacy, Changzhou University, Changzhou, Jiangsu, 213164, China.

Dual intramolecular FRET polymers are synthesized via Suzuki coupling and their luminescence characteristics from aggregation-caused quenching (ACQ) to aggregation-induced emission (AIE) is modulated conveniently by adjusting the charged ratios. The finally obtained AIE polymer is further employed to construct doxorubicin loaded nanoparticles as a promising theranostics platform for cancer therapy.
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http://dx.doi.org/10.1002/marc.202100264DOI Listing
October 2021

Ga-FAPI PET/CT Depicted Non-FDG-Avid Metastatic Appendiceal Mucinous Adenocarcinoma.

Authors:
Lin Qiu Yue Chen

Radiology 2021 10 3;301(1):45. Epub 2021 Aug 3.

From the Department of Nuclear Medicine, Affiliated Hospital of Southwest Medical University, No. 25 TaiPing St, Jiangyang District, Luzhou 646000, People's Republic of China; and Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, Luzhou, People's Republic of China.

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http://dx.doi.org/10.1148/radiol.2021210723DOI Listing
October 2021

A prospective study of dual-energy computed tomography for differentiating metastatic and non-metastatic lymph nodes of colorectal cancer.

Quant Imaging Med Surg 2021 Aug;11(8):3448-3459

Medical Imaging Center, The First Affiliated Hospital of Jinan University, Guangzhou, China.

Background: Colorectal cancer (CRC) is the third most common malignancy worldwide, and lymph node metastasis is considered to be a risk factor for local recurrence and a poor prognosis in colorectal cancer. However, there remains a lack of reliable and non-invasive biomarkers to identify the lymph node status of CRC patients preoperatively. The purpose of this study was to explore the ability of dual-energy computed tomography (DECT) to differentiate metastatic from non-metastatic lymph nodes in colorectal cancer.

Methods: Seventy-one patients with primary colorectal cancer underwent contrast-enhanced dual-energy computed tomography imaging preoperatively. The colorectal specimen was scanned postoperatively, and lymph nodes were matched to the pathology report. The following dual-energy computed tomography quantitative parameters were analyzed: dual-energy curve slope value (λ), standardized iodine concentration (n△HU), iodine water ratio (nIWR), electron density value (nρ), and effective atom-number (nZ), based on metastatic and non-metastatic lymph node differentiation. Also, sensitivity and specificity analyses were performed using receiver operating characteristic curves.

Results: In all patients, one hundred and fifty lymph nodes, including 66 non-metastatic and 84 metastatic lymph nodes, were matched using the radiological-pathological correlation. Metastatic nodes had significantly greater λ, n△HU, and nIWR values than non-metastatic nodes in both the arterial and venous phases (P<0.01). The area under curve (AUC), sensitivity, and specificity were 0.80, 80%, and 66% for λ; 0.86, 70%, and 95% for n△HU; and 0.88, 71%, and 95% for nIWR in the arterial phase. There was no significant difference in electron density and effective Z values between metastatic and non-metastatic lymph nodes.

Conclusions: DECT quantitative parameters may help differentiate between metastatic and normal lymph nodes in patients with CRC.
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http://dx.doi.org/10.21037/qims-20-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245958PMC
August 2021

Facial Nerve Monitoring under Different Levels of Neuromuscular Blockade with Cisatracurium Besilate in Parotid Tumour Surgery.

Biomed Res Int 2021 13;2021:5655061. Epub 2021 Jul 13.

Department of Anesthesiology, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Anaesthesia can alter neuronal excitability and vascular reactivity and ultimately lead to neurovascular coupling. Precise control of the skeletal muscle relaxant doses is the key in reducing anaesthetic damage.

Methods: A total of 102 patients with the normal functioning preoperative facial nerve who required parotid tumour resection were included in this study. Facial nerve monitoring was conducted intraoperatively. The surgeon stimulated the facial nerve at different myorelaxation intervals at TOF% (T4/T1) and T1% (T1/T0) and recorded the responses and the amplitude of electromyogram (EMG). Body movements (BM) or patient-ventilator asynchrony (PVA) was recorded intraoperatively.

Results: In parotid tumour resection, T1% should be maintained at a range of 30 to 60% while TOF% should be maintained at a range of 20 to 30%. Analysis of the decision tree model for facial nerve monitoring suggests a partial muscle relaxation level of 30% < T1% ≤ 50% and TOF ≤ 60%. A nomogram prediction model, while incorporating factors such as sex, age, BMI, TOF%, and T1%, was constructed to predict the risk of BM/PVA during surgery, showing good predictive performance.

Conclusions: This study revealed an adequate level of neuromuscular blockade in intraoperative parotid tumour resection while conducting facial nerve monitoring. A visual nomogram prediction model was constructed to guide anaesthetists in improving the anaesthetic plan.
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http://dx.doi.org/10.1155/2021/5655061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298146PMC
September 2021

The effects of resistance training on children with burns: a meta-analysis.

Pediatr Surg Int 2021 Oct 30;37(10):1323-1332. Epub 2021 Jul 30.

Department of Orthopedics, The Children's Hospital of Chongqing Medical University, 136 Zhongshan Second Road, Yuzhong District, Chongqing, 400014, China.

Aim: The aim of this study was to evaluate effectiveness and safety of resistance training (RT). Data were retrieved from Medline, EMBASE, PubMed, CINAHL and Cochrane Central Register of Controlled Trials (Central) databases from inception to April 2020. Quantitative studies on RT for muscle strength, lean body mass, cardiopulmonary function, metabolism, quality of life, and pain in burned children were included in this study. Twelve RCTs (379 patients) were identified. Meta-analysis showed RT significant increase in muscle strength [SMD = 2.18, 95% CI (0.79, 3.56), p = 0.002]. However, training showed no significant effect on muscle endurance [MD = 10.00, 95% CI (- 0.22, 20.22), p = 0.06]. Notably, training significantly increases total lean body mass [MD = 2.10, 95% CI (1.28, 2.92), p < 0.001]. In addition, training significantly increased leg lean body mass [MD = 2.10, 95% CI (1.28, 2.92), p < 0.001]. Moreover, training significantly increased VOpeak [MD = 5.83, 95% CI (3.52, 8.13), p < 0.001]. Meta-analysis showed that training significantly increases gait parameters, including stride length, step length, velocity and cadence. Furthermore, training significantly increased explosive capacity of lower limb muscles (p < 0.001). Meta-analysis of 6-min walking test results showed that training significantly improves walking speed (p = 0.0008). Notably, all studies showed unclear or high risk of bias; whereas, quality of the evidence was moderate or low. Analysis showed that RT significantly improves clinical outcomes. However, more high-quality, double-blind, randomized control trials should be performed to explore the effects of RT to ensure successful implementation in rehabilitation.
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http://dx.doi.org/10.1007/s00383-021-04947-6DOI Listing
October 2021

Manganese dioxide nanozyme for reactive oxygen therapy of bacterial infection and wound healing.

Biomater Sci 2021 Sep 28;9(17):5965-5976. Epub 2021 Jul 28.

School of Pharmacy, Changzhou University, Changzhou, Jiangsu 213164, China.

Reactive oxygen species (ROS) are the weapons of neutrophiles against bacterial pathogens, and also the central effectors in reactive oxygen therapy for skin and soft tissue infection. Nanozymes that spontaneously generate ROS under physiological conditions are new antibacterials that hold promise towards multidrug resistant pathogens. The clinical use of the nanozymes is however limited by their low biocompatibility and toxicity in vivo. Here, we develop an oleic acid (OA) nanoemulsion template method for the one-pot synthesis of OA-manganese dioxide (MnO) nanozyme. The OA-MnO nanozyme showed high stability and biocompatibility under physiological conditions with marked oxidase-like activity. The ROS generated by the OA-MnO nanozyme effectively kill the Gram-positive Staphylococcus aureus and the Gram-negative Escherichia coli strains. Moreover, the OA-MnO nanozyme shows promising abilities to prevent and destruct biofilm formation by Staphylococcus aureus, and result in superior in vivo antibacterial performance as compared to vancomycin. The reactive oxygen therapy based on OA-MnO nanozyme cures the infected skin and promotes wound healing in mice, manifesting its potential use in skin and soft tissue infection.
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http://dx.doi.org/10.1039/d1bm00683eDOI Listing
September 2021

Dermatomyositis on 68Ga-FAPI PET/CT in a Patient With Nasopharyngeal Carcinoma.

Clin Nucl Med 2021 Jul 20. Epub 2021 Jul 20.

From the Department of Nuclear Medicine, The Affiliated Hospital of Southwest Medical University Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province Academician (Expert) Workstation of Sichuan Province, Luzhou, Sichuan, China.

Abstract: Dermatomyositis is an idiopathic inflammatory myopathy often associated with malignancies. 68Ga-FAPI PET/CT was performed on a 58-year-old man with newly diagnosed dermatomyositis. 68Ga-FAPI PET/CT showed multiple increased FAPI activity in whole-body muscles and nasopharyngeal lesion. A biopsy of the nasopharyngeal lesion confirmed nasopharyngeal carcinoma. 68Ga-FAPI PET/CT can provide a "1-stop" imaging method for patients with dermatomyositis.
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http://dx.doi.org/10.1097/RLU.0000000000003809DOI Listing
July 2021

Occurrence of pesticides in white tea and a corresponding risk exposure assessment for the different residents in Fujian, China.

J Food Sci 2021 Aug 11;86(8):3743-3754. Epub 2021 Jul 11.

Fujian Key Laboratory of Agro-Products Quality and Safety, Institute of Quality Standards Testing Technology for Agro-products, Fujian Academy of Agricultural Sciences, Fuzhou, China.

White tea has been of increasing public interest worldwide owing to its health benefits. Based on 2 years of surveillance, the long-term and cumulative chronic exposure risks of pesticide residues through white tea drinking were assessed for different subpopulations in Fujian, China. Twenty-five different pesticides were found, and 74.8% of samples contained at least one pesticide residue. The most frequently detected pesticide was bifenthrin with detection rates of 61.6%. Risk assessment was performed using both the deterministic approach and semiprobabilistic model under the best-case and the worst-case scenarios. The results demonstrated that the dietary risks were extremely low for six different subpopulations in which the risks for adults over the age of 41 were relatively higher. The risk ranking scheme indicated that isocarbophos and triazophos were considered to be of medium risk. The different use suggestions for the 25 positive pesticides are proposed to further minimize the exposure risk to consumer health. PRACTICAL APPLICATION: Tea is the second most popular nonalcoholic beverage throughout the world. Pesticides are used to improve the yield of tea. Pesticide residues in tea could be one of the exposure pathways for consumers. Monitoring residual levels and assessing the health risk assessment in tea are thus in an urge.
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http://dx.doi.org/10.1111/1750-3841.15826DOI Listing
August 2021

Effect of socioeconomic characteristics and lifestyle on BMI distribution in the Chinese population: a population-based cross-sectional study.

BMC Public Health 2021 07 10;21(1):1369. Epub 2021 Jul 10.

School of Public Health, University of Alberta, Edmonton, Canada.

Background: Body mass index (BMI) is an accepted measurement that is widely used to quantify overweight and obesity at the population level. Previous studies have described the distribution variation of BMI through applying common statistical approaches, such as multiple linear or logistic regression analyses. This study proposed that associations between BMI and socioeconomic characteristics, diet, and lifestyle factors varied across the conditional BMI distribution.

Methods: This study was based on a sample of 10,023 Chinese adults who participated in the monitoring of chronic diseases and associated risk factors in Shaanxi Province, Northwest China, in 2013. Cross-quantile factors were observed in the relationships between major risk factors and BMI through quantile regression (QR) and ordinary least squares (OLS) regression.

Results: Participants' mean BMI was 24.19 ± 3.51 kg/m (range 14.33-52.82 kg/m). The QR results showed that living in urban areas was associated with BMI in the low and central quantiles (10th-60th). Participants with 6-9 years of education were 0.23-0.38 BMI units higher in the first half of the BMI quantiles compared with those with ≤6 years of education. There was a positive association between consumption of red meat and BMI; however, the association diminished from the 10th to the 50th quantile. Intake of oil and alcohol were positively associated with all BMI quantiles. Cigarette smoking per day was negatively associated with BMI, which showed a U-shaped distribution. The above results were also observed in the OLS.

Conclusion: This study implies that in addition to socioeconomic characteristics, limiting oil and alcohol intake may decrease BMI score. Consuming more red meat could be a strategy to increase BMI.
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http://dx.doi.org/10.1186/s12889-021-11405-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272370PMC
July 2021

Near infrared II laser controlled free radical releasing nanogenerator for synergistic nitric oxide and alkyl radical therapy of breast cancer.

Nanoscale 2021 Jul 17;13(25):11169-11187. Epub 2021 Jun 17.

Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Guangdong Provincial Engineering and Technological Research Center for Drug Carrier Development, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China.

Recently, alkyl radicals have attracted much attention in cancer therapy due to their oxygen-independent generation property. For the first time, alkyl radical and nitric oxide (NO) combined therapy is demonstrated as an effective strategy for tumor inhibition. As a proof of concept, a biocompatible free radical nanogenerator with near-infrared (NIR) II laser-induced simultaneous NO and alkyl radical release property was elaborately fabricated. In particular, an NIR II molecule (IR 1061), NO donor (BNN6) and alkyl radical initiator (AIPH) were firstly encapsulated in a natural lecithin stabilized phase change material, and then further functionalized by an amphiphile of DSPE-PEG-RGD with specific tumor targeting ability, finally obtaining biocompatible P(IR/BNN6/AIPH)@Lip-RGD. Upon NIR II laser irradiation, the photothermal effect generated from IR 1061 could trigger the phase change of the nanogenerator by releasing the encapsulated BNN6 and AIPH, and subsequently decompose them to generate highly active NO and alkyl radicals. Remarkably, NO and alkyl radical release profiles of P(IR/BNN6/AIPH)@Lip-RGD could be precisely controlled using intermittent NIR II laser irradiation. Moreover, P(IR/BNN6/AIPH)@Lip-RGD displayed a synergistic NO and alkyl radicals' anticancer effect by significantly inhibiting the growth of breast tumors, upon NIR II laser exposure. Furthermore, an in depth mechanistic study revealed that synergistic NO and alkyl radical effect induced cancer cell apoptosis through a mitochondria-mediated apoptotic pathway. The synergistic effect jointly caused a burst generation of mitochondrial ROS, which significantly down-regulated Bcl-2 protein expression, accelerated cytochrome c release and triggered a cascade of apoptosis-related proteins of Caspase-3 and Caspase-9.
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http://dx.doi.org/10.1039/d1nr01859kDOI Listing
July 2021

Sirt3 Protects Against Thoracic Aortic Dissection Formation by Reducing Reactive Oxygen Species, Vascular Inflammation, and Apoptosis of Smooth Muscle Cells.

Front Cardiovasc Med 2021 21;8:675647. Epub 2021 May 21.

Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

Sirtuin3 (Sirt3) is a histone deacetylase involved in the regulation of many cellular processes. Sirt3 deficiency is known to increase oxidative stress. Reactive oxygen species (ROS) promote degradation of the extracellular matrix and vascular smooth muscle cell (VSMC) apoptosis. Reducing oxidative stress by Sirt3 overexpression could have therapeutic potential for limiting thoracic aortic dissection (TAD) development. We hypothesized that Sirt3 deficiency could increase the risk for TAD by decreasing ROS elimination and that Sirt3 overexpression (Sirt3) could provide an alternative option for TAD treatment. Mice with TAD had significantly lower Sirt3 expression than normal subjects. Sirt3 KO mice exhibit significantly increased TAD incidence rate and increased aortic diameters. Moreover, Sirt3 overexpression reduced Ang II-induced ROS production, NF-kB activation, and apoptosis in human aortic smooth muscle cells (HASMCs). Sirt3 overexpression attenuated aneurysm formation and decreased aortic expansion. In conclusion, our data showed that Sirt3 deficiency increases susceptibility to TAD formation by attenuating anti-ROS effects and increasing VSMC apoptosis and vascular inflammation.
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http://dx.doi.org/10.3389/fcvm.2021.675647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176563PMC
May 2021

CBL-interacting protein kinase 31 regulates rice resistance to blast disease by modulating cellular potassium levels.

Biochem Biophys Res Commun 2021 Jul 28;563:23-30. Epub 2021 May 28.

College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address:

Rice blast disease caused by infection with Magnaporthe oryzae, a hemibiotrophic fungal pathogen, significantly reduces the yield production. However, the rice defense mechanism against blast disease remains elusive. To identify the genes involved in the regulation of rice defense to blast disease, dissociation (Ds) transposon tagging mutant lines were analyzed in terms of their response to M. oryzae isolate Guy11. Among them, CBL-interactingprotein kinase31 (CIPK31) mutants were more susceptible than wild-type plants to blast. The CIPK31 transcript was found to be insensitive to Guy11 infection, and the CIPK31-GFP was localized to the cytosol and nucleus. Overexpression of CIPK31 promoted rice defense to blast. Further analysis indicated that CIPK31 interacts with Calcineurin B-like 2 (CBL2) and CBL6 at the plasma membrane, and cbl2 mutants are more susceptible to blast compared with wild-type plants, suggesting that calcium signaling might partially through the CBL2-CIPK31 signaling regulate rice defense. Yeast two-hybrid results showed that AKT1-like (AKT1L), a potential potassium (K) channel protein, interacted with CIPK31, and the K level was significantly lower in the cipk31 mutants than in the wild-type control. In addition, exogenous potassium application increased rice resistance to blast, suggesting that CIPK31 might interact with AKT1L to increase K uptake, thereby promoting resistance to blast. Taken together, the results presented here demonstrate that CBL2-CIPK31-AKT1L is a new signaling pathway that regulates rice defense to blast disease.
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http://dx.doi.org/10.1016/j.bbrc.2021.05.065DOI Listing
July 2021

PPDPF alleviates hepatic steatosis through inhibition of mTOR signaling.

Nat Commun 2021 05 24;12(1):3059. Epub 2021 May 24.

CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.

Non-alcoholic fatty liver disease (NAFLD) has become the most prevalent chronic liver disease in the world, however, no drug treatment has been approved for this disease. Thus, it is urgent to find effective therapeutic targets for clinical intervention. In this study, we find that liver-specific knockout of PPDPF (PPDPF-LKO) leads to spontaneous fatty liver formation in a mouse model at 32 weeks of age on chow diets, which is enhanced by HFD. Mechanistic study reveals that PPDPF negatively regulates mTORC1-S6K-SREBP1 signaling. PPDPF interferes with the interaction between Raptor and CUL4B-DDB1, an E3 ligase complex, which prevents ubiquitination and activation of Raptor. Accordingly, liver-specific PPDPF overexpression effectively inhibits HFD-induced mTOR signaling activation and hepatic steatosis in mice. These results suggest that PPDPF is a regulator of mTORC1 signaling in lipid metabolism, and may be a potential therapeutic candidate for NAFLD.
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http://dx.doi.org/10.1038/s41467-021-23285-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144412PMC
May 2021

A New Oxaliplatin Resistance-Related Gene Signature With Strong Predicting Ability in Colon Cancer Identified by Comprehensive Profiling.

Front Oncol 2021 7;11:644956. Epub 2021 May 7.

Department of Colorectal Surgery, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.

Numerous colon cancer cases are resistant to chemotherapy based on oxaliplatin and suffer from relapse. A number of survival- and prognosis-related biomarkers have been identified based on database mining for patients who develop drug resistance, but the single individual gene biomarker cannot attain high specificity and sensitivity in prognosis prediction. This work was conducted aiming to establish a new gene signature using oxaliplatin resistance-related genes to predict the prognosis for colon cancer. To this end, we downloaded gene expression profile data of cell lines that are resistant and not resistant to oxaliplatin from the Gene Expression Omnibus (GEO) database. Altogether, 495 oxaliplatin resistance-related genes were searched by weighted gene co-expression network analysis (WGCNA) and differential expression analysis. As suggested by functional analysis, the above genes were mostly enriched into cell adhesion and immune processes. Besides, a signature was built based on four oxaliplatin resistance-related genes selected from the training set to predict the overall survival (OS) by stepwise regression and least absolute shrinkage and selection operator (LASSO) Cox analysis. Relative to the low risk score group, the high risk score group had dismal OS ( < 0.0001). Moreover, the area under the curve (AUC) value regarding the 5-year OS was 0.72, indicating that the risk score was accurate in the prediction of OS for colon cancer patients (AUC >0.7). Additionally, multivariate Cox regression suggested that the signature constructed based on four oxaliplatin resistance-related genes predicted the prognosis for colon cancer cases [hazard ratio (HR), 2.77; 95% CI, 2.03-3.78; < 0.001]. Finally, external test sets were utilized to further validate the stability and accuracy of oxaliplatin resistance-related gene signature for prognosis of colon cancer patients. To sum up, this study establishes a signature based on four oxaliplatin resistance-related genes for predicting the survival of colon cancer patients, which sheds more light on the mechanisms of oxaliplatin resistance and helps identify colon cancer cases with a dismal prognostic outcome.
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http://dx.doi.org/10.3389/fonc.2021.644956DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138443PMC
May 2021

Early wound repair versus later scar repair in children with treadmill hand friction burns.

J Burn Care Res 2021 May 20. Epub 2021 May 20.

Department of Burns and Plastic Surgery, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics.

The incidence of pediatric treadmill hand friction burns has been increasing every year. The injuries are deeper than thermal hand burns, the optimal treatment remains unclear. This was a retrospective study of children who received surgery for treadmill hand friction burns from January 1, 2015, to December 31, 2019, in a single burn center. A total of 22 children were surveyed. The patients were naturally divided into two groups: the wound repair group (13 patients), which was admitted early to the hospital after injury and received debridement and vacuum sealing drainage initially, and a full-thickness skin graft later; and the scar repair group (9 patients), in which a scar contracture developed as a result of wound healing and received scar release and skin grafting later. The Modified Michigan Hand Questionnaire score in the wound repair group was 116.31 ± 10.55, and the corresponding score in the scar repair group was 117.56 ± 8.85 (P>0.05), no statistically significant difference. The Vancouver Scar Scale score in the wound repair group was 4.15 ± 1.21, and the corresponding score in the scar repair group was 7.22 ± 1.09 (P<0.05). Parents were satisfied with the postoperative appearance and function of the hand. None in the two groups required secondary surgery. If the burns are deep second degree, third degree, or infected, early debridement, vacuum sealing drainage initially, and a full-thickness skin graft can obviously relieve pediatric pain, shorten the course of the disease, and restore the function of the hand as soon as possible.
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http://dx.doi.org/10.1093/jbcr/irab083DOI Listing
May 2021

Ac2-26 mitigated acute respiratory distress syndrome rats via formyl peptide receptor pathway.

Ann Med 2021 12;53(1):653-661

Department of Anesthesia, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Background: Acute respiratory distress syndrome (ARDS) is characterized by severe local and systemic inflammation. Ac2-26, an Annexin A1 Peptide, can reduce the lung injury induced by reperfusion via the inhibition of inflammation. The present study aims to evaluate the effect and mechanism of Ac2-26 in ARDS.

Methods: Thirty-two rats were anaesthetized and randomized into four groups: sham (S), ARDS (A), ARDS/Ac2-26 (AA), and ARDS/Ac2-26/BOC-2 (AAB) groups. Rats in the S group received saline for intratracheal instillation, while rats in the other three groups received endotoxin for intratracheal instillation, in order to prepare the ARDS and inject the saline, Ac2-26, and Ac2-26 combined with BOC-2. After 24 h, the PaO/FiO ratio was calculated. The lung tissue wet-to-dry weight ratio and the protein level in bronchoalveolar lavage fluid (BALF) were tested. Then, the cytokines in BALF and serum, and the inflammatory cells in BALF were investigated. Afterwards, the oxidative stress response and histological injury was evaluated. Subsequently, the epithelium was cultured and analyzed to estimate the effect of Ac2-26 on apoptosis.

Results: Compared to the S group, all indexes worsened in the A, AA, and AAB groups. Furthermore, compared to the S group, Ac2-26 significantly improved the lung injury and alveolar-capillary permeability, and inhibited the oxidative stress response. In addition, Ac2-26 reduced the local and systemic inflammation through the regulation of pro- and anti-inflammatory cytokines, and the decrease in inflammatory cells in BALF. Moreover, Ac2-26 inhibited the epithelium apoptosis induced by LPS through the modulation of apoptosis-regulated proteins. The protective effect of Ac2-26 on ARDS was partially reversed by the FPR inhibitor, BOC-2.

Conclusion: Ac2-26 reduced the lung injury induced by LPS, promoted alveolar-capillary permeability, ameliorated the local and systemic inflammation, and inhibited the oxidative stress response and apoptosis. The protection of Ac2-26 on ARDS was mainly dependent on the FPR pathway.
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http://dx.doi.org/10.1080/07853890.2021.1925149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143635PMC
December 2021

Gelatinase-responsive release of an antibacterial photodynamic peptide against .

Biomater Sci 2021 May;9(9):3433-3444

Department of Chemistry, the Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.

Staphylococcus aureus (S. aureus) related staphylococcal infection is one of the most common types of hospital-acquired infections, which requires selective and effective treatment in clinical practice. Considering gelatinase as a characteristic feature of S. aureus, gelatinase-responsive release of the antibiotic reagent thereby can target the pathogenic S. aureus while sparing beneficial bacteria in the microflora. In this work, we design a hybrid antibacterial photodynamic peptide (APP, Ce6-GKRWWKWWRRPLGVRGC) based on the polycationic antimicrobial peptide GKRWWKWWRR by introducing a photosensitizer chlorin e6 (Ce6) at the N-terminus, a cysteine residue at the C-terminus, and a gelatinase cleavage site (PLGVRG) inserted between the C-terminal cysteine and the polycationic peptide. This multi-motif peptide assembles with gold nanoclusters (AuNc) via Au-thiol bonding and affords a gelatinase-responsive antibacterial photodynamic nanocomposite (GRAPN). In vitro results show that the gelatinase secreted by S. aureus can cleave and release APP from AuNc, thereby resulting in preferential killing of S. aureus over E. coli. In a mouse model of staphylococcal skin wound infection, by integrating gelatinase-responsive drug release and the synergistic effect of a photodynamic agent and APP, GRAPN exhibits a marked photodynamic antibacterial activity, effectively eradicates S. aureus infection, and promotes rapid healing of the infected wounds.
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http://dx.doi.org/10.1039/d0bm02201bDOI Listing
May 2021

FKBP11 promotes cell proliferation and tumorigenesis via p53-related pathways in oral squamous cell carcinoma.

Biochem Biophys Res Commun 2021 06 1;559:183-190. Epub 2021 May 1.

Department of Oral and Maxillofacial Surgery, Tianjin Stomatological Hospital, School of Medicine, Nankai University, Tianjin, 300041, China; Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin, 300041, China. Electronic address:

Oral squamous cell carcinoma (OSCC) is one of the causes of cancer-related death worldwide. The abnormal proliferation ability of OSCC has become one of the major reasons for its poor prognosis. FK-506 binding protein 11 (FKBP11) is abnormally expressed in malignant tumors and affects many biological processes. The purpose of this study is to investigate the effect of FKBP11 on cell proliferation in OSCC and explore the possible regulatory mechanism. The expression of FKBP11 was detected by western blotting (WB) and/or real-time PCR in OSCC and paracancerous normal tissues in tongue squamous cell carcinoma (TSCC) cell lines, revealing high expression in OSCC and CAL-27 cells. Furthermore, FKBP11 knockdown inhibited the proliferation of CAL-27 cells by CCK-8 and colony formation assays. G/M arrest and induction of apoptosis were observed using flow cytometry, Hoechst 33258 and Calcein-AM/PI staining, accompanied by changes in some cell cycle- and apoptosis-related proteins, including CDK1, Cyclin B1, p21, p27, p53, Bax, Bcl-2 and Caspase-3. Additionally, the expression of these proteins can be reversed by the use of pifithrin-α (PFT-α), a p53 inhibitor. An in vivo xenograft model further confirmed that FKBP11 enhanced OSCC progression. In conclusion, FKBP11 could promote cell proliferation by regulating G/M phase and apoptosis via the p53/p21/p27 and p53/Bcl-2/Bax pathways, respectively, which suggests that it may be a new candidate target for the treatment of OSCC.
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http://dx.doi.org/10.1016/j.bbrc.2021.04.096DOI Listing
June 2021

Prevalence and Risk Factors of Hypertension, Diabetes, and Dyslipidemia among Adults in Northwest China.

Int J Hypertens 2021 9;2021:5528007. Epub 2021 Apr 9.

Shaanxi Provincial Center for Disease Control and Prevention, No. 3 Jiandong Street, Xi'an, China.

Objective: To understand the prevalence and its risk factors of hypertension, diabetes mellitus, and dyslipidemia among adults aged over 18 years in Northwest China and provide data for the prevention and control of chronic diseases in Northwest China.

Methods: Three waves of survey on chronic diseases and nutrition monitoring with multistage stratified cluster random sampling were conducted in 10 counties of Northwest China in 2013, 2015, and 2018, respectively. Personal information, socioeconomic status (SES), and behavioral risk factors (cigarettes smoking, alcohol consumption, diets, and physical activity) were collected by face-to-face interview. Height, weight, and blood pressure were measured, and blood glucose and serum lipid were tested. Prevalence of hypertension, diabetes, and dyslipidemia of the three waves was estimated, and multivariate logistic regression was used to analyze their risk factors.

Results: The prevalence of hypertension, diabetes, and dyslipidemia was 41.59%, 11.16%, and 32.48%, respectively. Their standardized prevalence was 29.31%, 7.94%, and 31.54%. Univariate analysis showed that the prevalence of hypertension, diabetes, and dyslipidemia was significantly different among educational levels, marital status, occupation, smoking, drinking, central obesity, and BMI classification ( < 0.05). Multivariate logistic regression analysis showed that factors like male gender, central obesity, overweight, and obesity were associated with hypertension, diabetes, and dyslipidemia ( < 0.05). High BMI index is one of the risk factors of the three diseases. The odds ratio (OR) of general overweight associated with hypertension, diabetes, and dyslipidemia was 1.663, 1.206, and 1.579 compared to normal body weight, respectively. And that was 3.094, 1.565, and 2.285 for obesity. Age was one of the risk factors for hypertension and diabetes ( < 0.05). Age groups of 45-59 years and 60 years and above were more associated with hypertension than of 18-44 age group (OR = 2.777, OR = 6.948), similar to their association with diabetes (OR = 2.357, OR = 3.521). Daily smoking is associated with diabetes and dyslipidemia (OR  = 1.217, OR  = 1.287) and alcohol drinking associated with hypertension and dyslipidemia (OR  = 1.014, OR  = 1.034). Hypertension, diabetes, and dyslipidemia were also associated with each other ( < 0.05). The number of participants with hypertension, diabetes, and dyslipidemia accounted for 2.33% of all the participants, 15.60% for participants with hypertension and dyslipidemia, 4.58% for hypertension and diabetes, and 3.57% for diabetes and dyslipidemia, respectively.

Conclusion: Factors like male, smoking, drinking, central obesity, overweight, and obesity were associated with hypertension, diabetes, and dyslipidemia in northwest China. Interventions on these risk factors and coexistence of the three diseases may help improve public health in this area.
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http://dx.doi.org/10.1155/2021/5528007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055385PMC
April 2021

Preparation, characterization and dyeing wastewater treatment of a new PVDF/PMMA five-bore UF membrane with β-cyclodextrin and additive combinations.

Water Sci Technol 2021 Apr;83(8):1847-1862

Donghua University, Shanghai 200051, China.

A new type of polyvinylidene fluoride (PVDF)/polymethyl methacrylate (PMMA) hollow fiber membrane (HFM) with five bores was prepared. The effects of polyvinylpyrrolidone (PVP), β-cyclodextrine (β-CD), polyethylene glycol (PEG) and polysorbate 80 (Tween 80) and their combinations on the PVDF/PMMA five-bore HFMs were investigated. The performance and fouling characteristics of five-bore HFMs for dyeing wastewater treatment were evaluated. Results indicated that adding 5 wt.% PVP increased the porosity and water flux of the membrane but decreased the bovine serum albumin (BSA) rejection rate. Adding 5 wt.% β-CD significantly improved the tensile strength and rejection of the HFMs with no effect on the increase of water flux. The characteristic of the HFMs with different additive combinations proved that the mixture of 5 wt.% PVP and 1 wt.% β-CD gave the best membrane performance, with a pure water flux of 427.9 L/ m·h, a contact angle of 25°, and a rejection of BSA of 89.7%. The COD and UV removal rates of dyeing wastewater treatment were 61.10% and 50.41%, respectively. No breakage or leakage points were found after 120 days of operation, showing their reliable mechanical properties. We set the operating flux to 55 L/m·h and cross-flow rate to 10%, which can effectively control membrane fouling.
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http://dx.doi.org/10.2166/wst.2021.104DOI Listing
April 2021

Silencing of SNHG6 alleviates hypoxia/reoxygenation-induced cardiomyocyte apoptosis by modulating miR-135a-5p/HIF1AN to activate Shh/Gli1 signalling pathway.

J Pharm Pharmacol 2021 Mar;73(1):22-31

Department of Cardiology, People's Hospital of Zhengzhou University, Henan Provincial People's Hospital, Fuwai Central China Cardiovascular Hospital, Zhengzhou, China.

Objectives: To examine the effects of small nucleolar RNA host gene 6 (SNHG6) on apoptosis during myocardial ischemic/reperfusion (I/R) injury and its potential molecular mechanisms.

Methods: In vitro model of I/R was built through exposing mouse HL-1 cardiomyocytes to hypoxia/reoxygenation (H/R) treatment. Quantitative real-time polymerase chain reaction assays were performed to determine gene expression. Cell Counting Kit-8, flow cytometric and western blot assays were conducted to detect cell viability, apoptosis and protein expression. Lactate dehydrogenase (LDH) activity was examined by a commercial detection kit. Dual-luciferase gene reporter and RNA immunoprecipitation experiments were applied for determining the interaction between the molecules.

Key Findings: SNHG6 expression was increased in H/R-challenged cardiomyocytes. Depletion of SNHG6 protected against H/R-induced cardiomyocytes apoptosis. SNHG6 could sponge miR-135a-5p to inhibit its expression. Down-regulation of miR-135a-5p reversed the anti-apoptotic effect caused by SNHG6 knockdown in H/R-induced cardiomyocytes. Hypoxia inducible factor 1 subunit alpha inhibitor (HIF1AN) was identified as a direct target of miR-135a-5p, and knockdown of HIF1AN relieved H/R-induced cardiomyocytes apoptosis. Silencing of SNHG6 activated Shh/Gli1 signalling pathway by regulating miR-135a-5p/HIF1AN. Furthermore, inactivation of Shh/Gli signalling abolished the anti-apoptotic effects of SNHG6 knockdown in H/R-induced cardiomyocytes.

Conclusions: SNHG6 serves as a sponge for miR-135a-5p to promote HIF1AN expression and inactivate Shh/Gli1 signalling, eventually aggravating H/R-induced apoptosis in cardiomyocytes.
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http://dx.doi.org/10.1093/jpp/rgaa064DOI Listing
March 2021

Fluorescence coupled capillary electrophoresis as a strategy for tetrahedron DNA analysis.

Talanta 2021 Jun 19;228:122225. Epub 2021 Feb 19.

School of Pharmacy, Changzhou University, Changzhou, Jiangsu, 213164, China; Changzhou Le Sun Pharmaceuticals Co., Ltd., Changzhou, Jiangsu, 213125, China; Jiangsu Yue Zhi Biopharmaceutical Co., Ltd., Changzhou, Jiangsu, 213125, China. Electronic address:

A strategy based on fluorescence coupled capillary electrophoresis (CE-FL) was developed for analyzing tetrahedron DNA (TD) and TD-doxorubicin (DOX) conjugate. Capillary gel electrophoresis exhibited desirable performance for separating TD and DNA strands. Under the optimized conditions, satisfactory repeatability concerning run-to-run and interday repeatability was obtained, and relative standard deviation value of resolution (n = 6) was 0.64%. Furthermore, the combination of CE and fluorescence detection provided a sensitive platform for quantifying TD concentration and calculating the damage degree of TD. The electrophoretograms indicated that CE-FL was a suitable TD assay method with high specificity and sensitivity. In addition, the application of CE-FL for TD fluorescence resonance energy transfer (FRET) research was also explored. Two types of DNA strands were utilized to interfere the formation of TD. The impact of partially complementary chain and completely complementary chain on FRET signal was explored, and the influence mechanism was discussed. After applying CE-FL for characterizing TD, we also combine CE and FRET to analyze TD-DOX conjugate. CE presented a favourable technique to monitor DOX loading and releasing processes. These noteworthy results offered a stepping stone for DNA nanomaterials assay by using CE-FL.
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http://dx.doi.org/10.1016/j.talanta.2021.122225DOI Listing
June 2021
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