Publications by authors named "Lin Luo"

455 Publications

cMOS enhanced the mucosal immune function of skin and gill of goldfish (Carassius auratus Linnaeus) to improve the resistance to Ichthyophthirius multifiliis infection.

Fish Shellfish Immunol 2022 May 17. Epub 2022 May 17.

Department of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.

Methods: of supporting mucosal immune barrier integrity and prevention of some pathogenic infections in aquatic species, are key areas of active study, often focusing on feed additives. The objectives of this study were to explore the effects of feeding cMOS (concentrated mannan oligosaccharide) on the gill and skin mucosal barriers of goldfish (Carassius auratus Linnaeus) and evaluate health status during Ichthyophthirius multifiliis infection. After feeding the cMOS-containing diet for 60 days, Hematoxylin and eosin (H&E) staining showed greater length of gill lamella and thicker dermal dense layer, while Alcian Blue and Periodic acid-Schiff (AB-PAS) staining showed higher numbers of mucin cells in cMOS fed fish. Chemical analysis showed that fish fed cMOS had greater enzyme activity of lysozyme (LZM) and alkaline phosphatase (AKP) in gill and skin tissues, while qRT-PCR revealed higher expression of Muc-2 and IL-1β, as well as lower expression of IL-10. After Ichthyophthirius multifiliis challenge, goldfish fed the cMOS diet had lower mortality and infection rates, as well as fewer visible white spots on the body surfaces. Histologically, the gill and skin of these fish presented less tissue damage and fewer parasites, and had a greater number of mucus cells. In addition, the expression of Muc-2 and IL-10 were notably higher while the expression of IL-1β was significantly lower in cMOS fed goldfish than control fed fish. In this study, cMOS fed goldfish had stronger immune barrier function of skin and gill mucous, and better survival following Ichthyophthirius multifiliis infection.
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http://dx.doi.org/10.1016/j.fsi.2022.05.024DOI Listing
May 2022

The characteristics of alkaline phosphatase activity and phoD gene community in heavy-metal contaminated soil remediated by biochar and compost.

Bull Environ Contam Toxicol 2022 May 13. Epub 2022 May 13.

College of Resources and Environment, Hunan Agricultural University, 410128, Changsha, China.

This research was carried out to determine the influence of biochar and compost addition on the characteristics of potential alkaline phosphatase (ALP) activity and phoD gene community in heavy metal polluted soils. The ALP activity, the abundance and structure of phoD gene were systematically determined. Results showed that biochar and compost significantly changed soil properties, and promoted the microbial transformation of phosphorus. Compost addition significantly increased the ALP activity. Biochar and compost addition markedly increased the phoD gene abundance. The addition of biochar increased the proportion of Actinobacteria, Euryarchaeota, and Proteobacteria. By contrast, Betaproteobacteria, Deltaproteobacteria, and Gammaproteobacteria were the dominant taxa in soils with compost addition. Electrical conductivity critically controlled the expression of phoD and changed the structure of phoD-coding microbial communities in heavy-metal polluted soils that remediated by biochar and compost.
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http://dx.doi.org/10.1007/s00128-022-03513-7DOI Listing
May 2022

A Convolutional Neural Network for Ultrasound Plane Wave Image Segmentation with a Small Amount of Phase Array Channel Data.

IEEE Trans Ultrason Ferroelectr Freq Control 2022 May 12;PP. Epub 2022 May 12.

Single angle plane wave has a huge potential in ultrasound high frame rate imaging, which, however, has a number of difficulties such as low imaging quality and poor segmentation results. To overcome these difficulties, an end-to-end convolutional neural network (CNN) structure from single-angle channel data was proposed to segment images in this paper. The network removed the traditional beamforming process and used raw radio frequency (RF) data as input to directly obtain segmented image. The signal features at each depth were extracted and concatenated to obtain the feature map by a special depth signal extraction module, the feature map was then put into the residual encoder and decoder to obtain the output. A simulated hypoechoic cysts data set of 2000 and an actual industrial defect dataset of 900 were used for training separately. Good results have been achieved in both simulated medical cysts segmentation and actual industrial defects segmentation. Experiments were conducted on both datasets with phase array sparse element data as input, and segmentation results were obtained for both. On the whole, this work achieved better quality segmented images with shorter processing time from single-angle plane wave channel data using convolutional neural networks, compared with other methods, our network has been greatly improved in intersection over union (IOU), F1-score, and processing time. And it indicated that the feasibility of applying deep learning in image segmentation can be improved by using phase array sparse element data as input.
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http://dx.doi.org/10.1109/TUFFC.2022.3174637DOI Listing
May 2022

Effects of mycorrhiza and hyphae on the response of soil microbial community to warming in eastern Tibetan Plateau.

Sci Total Environ 2022 May 4;837:155498. Epub 2022 May 4.

State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil & Water Pollution, College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, PR China. Electronic address:

The effects of mycorrhiza and its external hyphae on the response of soil microbes to global warming remain unclear. This study investigates the role of mycorrhiza and its hyphae in regulating soil microbial community under warming by examining the microbial biomass and composition in the ingrowth cores of arbuscular mycorrhiza (AM) plant, Fargesia nitida, and ectomycorrhiza (ECM) plant, Picea asperata, with/without mycorrhiza/hyphae and experimental warming. The results showed that warming significantly increased the biomass of all soil microbes (by 19.89%-137.48%) and altered the microbial composition in both plant plots without mycorrhiza/hyphae. However, this effect was weakened in the presence of mycorrhiza or hyphae. In F. nitida plots, warming did not significantly affect biomass and composition of most soil microbial groups when mycorrhiza or hyphae were present. In P. asperata plots, warming significantly increased the total and ECM fungi (ECMF) biomass in the presence of hyphae (p < 0.05) and the total, Gn, and AM fungi (AMF) biomass in the presence of mycorrhiza (p < 0.05). Meanwhile, the response of enzyme activities to warming was also altered with mycorrhiza or hyphae. Additionally, soil microbial community composition was mainly influenced by soil available phosphorus (avaP), while enzyme activities depended on soil avaP, dissolved organic carbon (DOC), and nitrate concentrations. Our results indicate that mycorrhiza and its hyphae are essential in regulating the response of microbes to warming.
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http://dx.doi.org/10.1016/j.scitotenv.2022.155498DOI Listing
May 2022

Forsythiae Fructuse extracts alleviates LPS-induced acute lung injury in mice by regulating PPAR-γ/RXR-α in lungs and colons.

J Ethnopharmacol 2022 Jul 26;293:115322. Epub 2022 Apr 26.

State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China; School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China. Electronic address:

Ethnopharmacological Relevance: Forsythiae Fructuse (FF), the dried fruit of Forsythia suspensa (Thunb.) Vahl, is used as a traditional Chinese medicine that has been reported to exert good anti-inflammatory effects in the treatment of many lung diseases.

Aim Of The Study: The purpose of this study was to investigate the anti-inflammatory mechanism of FF in the treatment of acute lung injury (ALI) based on gut-lung axis.

Materials And Methods: ALI model was established by the intratracheal instillation of 5 mg/kg LPS in ICR mice. Mice were administered intragastrically with dexamethasone (DEX), and low-dose, medium-dose and high-dose of FF extracts (LFF, MFF and HFF) in addition to the mice of control (CON) and model (MOD) groups. Pathological observation and inflammation scoring of lung tissues were based on HE staining. Limulus lysate assay was used to detect endotoxin levels in serum. Western blot and Real-time quantitative PCR were respectively applied to detect the protein and mRNA expressions in both lung and colon tissues.

Results: Lung pathological injury, inflammatory score and inflammatory genes (IL-6, IL-1β, TNF-α) could be effectively suppressed by FF in LPS-induced ALI mice. FF also increased the proteins of epithelial markers (E-cadherin, ZO-1 and Claudin-1) in lung and colon tissues, and decreased colonic inflammatory genes for protecting the epithelial barriers of lung and colon. The protein expression of TLR4/MAPK/NF-κB inflammatory signaling pathway in lung and colon was significantly inhibited by FF via the regulation of PPAR-γ, a nuclear hormone receptor that forms the heterodimer with RXR-α to inhibit inflammatory gene transcription. More specifically, FF promoted the upregulation of protein, phosphorylated proteins and genes of PPAR-γ/RXR-α in lungs, while inhibited the protein overexpression and phosphorylation of PPAR-γ/RXR-α in colons.

Conclusions: FF exhibited anti-inflammatory effects and protected the epithelial barriers in lungs and colons by regulating PPAR-γ/RXR-α in the treatment of LPS-induced ALI.
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http://dx.doi.org/10.1016/j.jep.2022.115322DOI Listing
July 2022

Pan-Cancer Analysis Shows That ALKBH5 Is a Potential Prognostic and Immunotherapeutic Biomarker for Multiple Cancer Types Including Gliomas.

Front Immunol 2022 4;13:849592. Epub 2022 Apr 4.

Tianjin Neurological Institute, Key Laboratory of Post-Neuroinjury Neuro-repair and Regeneration in Central Nervous System, Ministry of Education and Tianjin City, Tianjin Medical University General Hospital, Tianjin, China.

Background: AlkB homolog 5 (ALKBH5) is a N-methyladenosine (mA) demethylase associated with the development, growth, and progression of multiple cancer types. However, the biological role of ALKBH5 has not been investigated in pan-cancer datasets. Therefore, in this study, comprehensive bioinformatics analysis of pan-cancer datasets was performed to determine the mechanisms through which ALKBH5 regulates tumorigenesis.

Methods: Online websites and databases such as NCBI, UCSC, CCLE, HPA, TIMER2, GEPIA2, cBioPortal, UALCAN, STRING, SangerBox, ImmuCellAl, xCell, and GenePattern were used to extract data of ALKBH5 in multiple cancers. The pan-cancer patient datasets were analyzed to determine the relationship between ALKBH5 expression, genetic alterations, methylation status, and tumor immunity. Targetscan, miRWalk, miRDB, miRabel, LncBase databases and Cytoscape tool were used to identify microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) that regulate expression of ALKBH5 and construct the lncRNA-miRNA-ALKBH5 network. CCK-8, wound healing, Transwell and M2 macrophage infiltration assays as well as xenograft animal experiments were performed to determine the biological functions of ALKBH5 in glioma cells.

Results: The pan-cancer analysis showed that ALKBH5 was upregulated in several solid tumors. ALKBH5 expression significantly correlated with the prognosis of cancer patients. Genetic alterations including duplications and deep mutations of the gene were identified in several cancer types. Alterations in the gene correlated with tumor prognosis. GO and KEGG enrichment analyses showed that ALKBH5-related genes were enriched in the inflammatory, metabolic, and immune signaling pathways in glioma. ALKBH5 expression correlated with the expression of immune checkpoint (ICP) genes, and influenced sensitivity to immunotherapy. We constructed a lncRNA-miRNA network that regulates ALKBH5 expression in tumor development and progression.  and experiments showed that ALKBH5 promoted proliferation, migration, and invasion of glioma cells and recruited the M2 macrophage to glioma cells.

Conclusions: ALKBH5 was overexpressed in multiple cancer types and promoted the development and progression of cancers through several mechanisms including regulation of the tumor-infiltration of immune cells. Our study shows that ALKBH5 is a promising prognostic and immunotherapeutic biomarker in some malignant tumors.
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http://dx.doi.org/10.3389/fimmu.2022.849592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013910PMC
April 2022

Initiating a high-temperature zinc ion battery through a triazolium-based ionic liquid.

RSC Adv 2022 Mar 16;12(14):8394-8403. Epub 2022 Mar 16.

College of Chemistry and Chemical Engineering, Jishou University Jishou 416000 China

Triazolium-based ionic liquids (T1, T2 and T3) with or without terminal hydroxyl groups were prepared Cu(i) catalysed azide-alkyne click chemistry and their properties were investigated using various technologies. The hydroxyl groups obviously affected their physicochemical properties, where with a decrease in the number of hydroxyl groups, their stability and conductivity were enhanced. T1, T2 and T3 showed relatively high thermal stability, and their electrochemical stability windows (ESWs) were 4.76, 4.11 and 3.52 V, respectively. T1S-20 was obtained the addition of zinc trifluoromethanesulfonic acid (Zn(CFSO)) and lithium bis(trifluoromethanesulfonyl)imide (LiTFSI) to T1, displaying conductivity and ESW values of 1.55 × 10 S cm and 6.36 V at 30 °C, respectively. Subsequently, a Zn/LiV(PO) battery was assembled using T1S-20 as the electrolyte and its performances at 30 °C and 80 °C were investigated. The battery showed a capacity of 81 mA h g at 30 °C, and its capacity retention rate was 89% after 50 cycles. After increasing the temperature to 80 °C, its initial capacity increased to 111 mA h g with a capacity retention rate of 93.6% after 100 cycles, which was much higher than that of the aqueous electrolyte (WS-20)-based zinc ion battery (71.8%). Simultaneously, the T1S-20 electrolyte-based battery exhibited a good charge/discharge efficiency, and its Coulomb efficiency was 99%. Consequently, the T1S-20 electrolyte displayed a better performance in the Zn/LiV(PO) battery than that with the aqueous electrolyte, especially at high temperature.
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http://dx.doi.org/10.1039/d2ra00298aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8984945PMC
March 2022

Bacillus amyloliquefaciens as an excellent agent for biofertilizer and biocontrol in agriculture: An overview for its mechanisms.

Microbiol Res 2022 Jun 29;259:127016. Epub 2022 Mar 29.

CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu 610041, PR China. Electronic address:

Bacillus amyloliquefaciens (BA) is one of the most promising bacteria for plant growth promotion (PGP) without harmful side effects. As an excellent agent for biofertilizer and biocontrol in agriculture, the PGP mechanisms of BA have been studied extensively. However, these studies have been rarely summarized, although it could hinder a better understanding of BA strains' potential mechanisms and application in agriculture and other fields. Hence, we reviewed in this work the PGP mechanisms of BA and the current limits of BA application in agriculture. First, BA can improve soil nutrient availability, including improving nitrogen supply, solubilizing phosphate and potassium, and producing siderophores. Second, BA can change the soil microbial community and improve the availability of minerals and plant growth conditions. Third, BA can secrete hormones and volatile organic compounds (VOCs) associated with plant cell growth and root development and further improve nutrient uptake by plants. Fourth, BA can enhance plant resistance against biotic stresses from soil pathogens through competition of niches and nutrients, producing substances such as cyclic lipopeptides, polyketides, and VOCs to antagonize pathogens directly, and induction of system resistance of the plants. Similarly, inoculation with BA can promote plant growth by inducing systematic tolerance to abiotic stresses by leading to genetic, chemical, and physical changes in the host plant. We further suggested that, in future studies, more attention should be paid to nutrient uptake mechanisms of plants with advanced techniques in different soil conditions and fields.
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http://dx.doi.org/10.1016/j.micres.2022.127016DOI Listing
June 2022

Prediction of Pulmonary Fibrosis Based on X-Rays by Deep Neural Network.

J Healthc Eng 2022 26;2022:3845008. Epub 2022 Mar 26.

The First Affiliated Hospital of Dalian Medical University, Dalian 116011, China.

As a fatal lung disease, pulmonary fibrosis can cause irreversible damage to the lung, affect normal lung function, and eventually lead to death. At present, the pathogenesis of this kind of disease is not completely clear, and there is no radical cure. The main purpose of the treatment of this disease is to slow down the deterioration of pulmonary fibrosis. For this kind of disease, if it can be found early, it can be treated as soon as possible and the life of patients will be prolonged. Clinically, the diagnosis of pulmonary fibrosis depends on the relevant imaging examination, lung biopsy, lung function examination, and so on. Imaging data such as X-rays is a common examination means in clinical medicine and also plays an important role in the prediction of pulmonary fibrosis. Through X-ray, radiologists can clearly see the relevant lung lesions so as to make the relevant diagnosis. Based on the common medical image data, this paper designs related models to complete the prediction of pulmonary fibrosis. The model designed in this paper is mainly divided into two parts: first, this paper uses a neural network to complete the segmentation of lung organs; second, the neural network of image classification is designed to complete the process from lung image to disease prediction. In the design of these two parts, this paper improves on the basis of previous research methods. Through the design of a neural network with higher performance, more optimized results are achieved on the key indicators which can be applied to the real scene of pulmonary fibrosis prediction.
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http://dx.doi.org/10.1155/2022/3845008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8976624PMC
March 2022

Kimura's disease successively affecting multiple body parts: a case-based literature review.

BMC Ophthalmol 2022 Apr 2;22(1):154. Epub 2022 Apr 2.

Department of Ophthalmology, Longgang District People's Hospital of Shenzhen, No.53 Aixin Road, Longgang District, Shenzhen, 518172, China.

Background: Kimura's disease is a rare, benign, chronic inflammatory disease that presents as painless, solid masses mainly affecting the deep subcutaneous areas of the head and neck, especially the salivary glands, parotid glands and nearby lymph nodes. It is characterized by elevated peripheral blood eosinophil and Immunoglobulin E (IgE) levels.

Case Presentation: A 31-year-old Asian male presented with an orbital space-occupying lesion lasting for 1.5 years. Ten years prior, surgical excision of bilateral fossa cubitalis and groin masses was performed, and the pathological examination showed "lymphoproliferative disease". One year later, masses reappeared near the surgical sites; they grew slowly and shrank after glucocorticoid treatment. At this point, admission examinations showed in the peripheral blood an eosinophil proportion of 13.4%, a total IgE level of 26,900.00 IU/mL, prurigo present on the whole body, and multiple palpable masses near the bilateral fossa cubitalis and groin. The left eyeball was exophthalmic. The left elbow mass was excised, and the pathological examination confirmed Kimura's disease. Oral glucocorticoid therapy is taken and tapering regularly. The eosinophil count returned to normal, the IgE level gradually decreased, the orbital space-occupying lesion and elbow and groin masses shrank significantly, and the whole-body skin prurigo disappeared. Currently, the patient has been in a stable condition for eighteen months.

Conclusion: Our case provides a novel insight that Kimura's disease should be involved in the differential diagnosis of inflammatory lesion mass of orbit and also supports systemic regular glucocorticoid as a valuable therapy of such condition, but close follow-up and long-term observation are crucial.
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http://dx.doi.org/10.1186/s12886-022-02378-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977031PMC
April 2022

The transmembrane adapter SCIMP recruits tyrosine kinase Syk to phosphorylate Toll-like receptors to mediate selective inflammatory outputs.

J Biol Chem 2022 Mar 22;298(5):101857. Epub 2022 Mar 22.

Institute for Molecular Bioscience (IMB) and IMB Centre for Inflammation and Disease Research, The University of Queensland; Brisbane, Queensland, Australia. Electronic address:

Innate immune signaling by Toll-like receptors (TLRs) involves receptor phosphorylation, which helps to shape and drive key inflammatory outputs, yet our understanding of the kinases and mechanisms that mediate TLR phosphorylation is incomplete. Spleen tyrosine kinase (Syk) is a nonreceptor protein tyrosine kinase, which is known to relay adaptive and innate immune signaling, including from TLRs. However, TLRs do not contain the conserved dual immunoreceptor tyrosine-based activation motifs that typically recruit Syk to many other receptors. One possibility is that the Syk-TLR association is indirect, relying on an intermediary scaffolding protein. We previously identified a role for the palmitoylated transmembrane adapter protein SCIMP in scaffolding the Src tyrosine kinase Lyn, for TLR phosphorylation, but the role of SCIMP in mediating the interaction between Syk and TLRs has not yet been investigated. Here, we show that SCIMP recruits Syk in response to lipopolysaccharide-mediated TLR4 activation. We also show that Syk contributes to the phosphorylation of SCIMP and TLR4 to enhance their binding. Further evidence pinpoints two specific phosphorylation sites in SCIMP critical for its interaction with Syk-SH2 domains in the absence of immunoreceptor tyrosine-based activation motifs. Finally, using inhibitors and primary macrophages from SCIMP mice, we confirm a functional role for SCIMP-mediated Syk interaction in modulating TLR4 phosphorylation, signaling, and cytokine outputs. In conclusion, we identify SCIMP as a novel, immune-specific Syk scaffold, which can contribute to inflammation through selective TLR-driven inflammatory responses.
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http://dx.doi.org/10.1016/j.jbc.2022.101857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9052152PMC
March 2022

Production and Characterization of Biotinylated Anti-fenitrothion Nanobodies and Development of Sensitive Fluoroimmunoassay.

J Agric Food Chem 2022 Apr 25;70(13):4102-4111. Epub 2022 Mar 25.

Guangdong Provincial Key Laboratory of Food Quality and Safety/Research Center for Green Development of Agriculture, South China Agricultural University, Guangzhou 510642, China.

A simple and sensitive fluoroimmunoassay (FIA) based on a heavy-chain antibody (VHH) for rapid detection of fenitrothion was developed. A VHH library was constructed from an immunized alpaca, and one clone recognizing fenitrothion (namely, VHHjd8) was achieved after careful biopanning. It was biotinylated by fusing with the Avi tag and biotin ligase to obtain a fusion protein (VHHjd8-BT), showing both binding capacity to fenitrothion and the streptavidin poly-horseradish peroxidase conjugate (SA-polyHRP). Based on a competitive assay format, the absorbance spectrum of oxidized 3,3',5,5'-tetramethylbenzidine generated by SA-polyHRP overlapped the emission spectrum of carbon dots, which resulted in quenching of signals due to the inner-filter effect. The developed FIA showed an IC value of 1.4 ng/mL and a limit of detection of 0.03 ng/mL, which exhibited 15-fold improvement compared with conventional enzyme-linked immunosorbent assay. The recovery test of FIA was validated by standard GC-MS/MS, and the results showed good consistency, indicating that the assay is an ideal tool for rapid screening of fenitrothion in bulk food samples.
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http://dx.doi.org/10.1021/acs.jafc.2c00826DOI Listing
April 2022

MAFFNet: real-time multi-level attention feature fusion network with RGB-D semantic segmentation for autonomous driving.

Appl Opt 2022 Mar;61(9):2219-2229

Compared with RGB semantic segmentation, RGB-D semantic segmentation can combine the geometric depth information to effectively improve the segmentation accuracy. Considering the application of RGB-D semantic segmentation in autonomous driving, we design a real-time semantic segmentation network, that is, MAFFNet, which can effectively extract depth features and combine the complementary information in RGB and depth. We also design a multi-level attention feature fusion module that can excavate the available context information of RGB and depth features. At the same time, its inference speed can also meet the demands of autonomous driving. Experiments show that our network achieves excellent performance of 74.4% mIoU and an inference speed of 15.9 Hz at a full resolution of 2048×1024 on the cityscapes dataset. Using multi-source learning, we mixed the cityscapes and lost and found as the multi-dataset. Our network is also superior to previous algorithms in using the multi-dataset to detect small obstacles outside the road.
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http://dx.doi.org/10.1364/AO.449589DOI Listing
March 2022

Rapid and Wash-Free Time-Gated FRET Histamine Assays Using Antibodies and Aptamers.

ACS Sens 2022 Apr 21;7(4):1113-1121. Epub 2022 Mar 21.

Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China.

Histamine (HA) is an indicator of food freshness and quality. However, high concentrations of HA can cause food poisoning. Simple, rapid, sensitive, and specific quantification can enable efficient screening of HA in food and beverages. However, conventional assays are complicated and time-consuming, as they require multiple incubation, washing, and separation steps. Here, we demonstrate that time-gated Förster resonance energy transfer (TG-FRET) between terbium (Tb) complexes and organic dyes can be implemented in both immunosensors and aptasensors for simple HA quantification using a rapid, single-step, mix-and-measure assay format. Both biosensors could quantify HA at concentrations relevant in food poisoning with limits of detection of 0.19 μg/mL and 0.03 μg/mL, respectively. Excellent specificity was documented against the structurally similar food components tryptamine and l-histidine. Direct applicability of the TG-FRET assays was demonstrated by quantifying HA in spiked fish and wine samples with both excellent concentration recovery and agreement with conventional multistep enzyme-linked immunosorbent assays (ELISAs). Our results show that the simplicity and rapidity of TG-FRET assays do not compromise sensitivity, specificity, and reliability, and both immunosensors and aptasensors have a strong potential for their implementation in advanced food safety screening.
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http://dx.doi.org/10.1021/acssensors.2c00085DOI Listing
April 2022

Conversion of Cellulose and Lignin Residues into Transparent UV-Blocking Composite Films.

Molecules 2022 Mar 1;27(5). Epub 2022 Mar 1.

Chengdu Campus, Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, China.

The valorization of cellulose and lignin residues in an integrated biorefinery is of great significance to improve the overall economics but has been challenged by their structural recalcitrance, especially for lignin residue. In this work, a facile chemical conversion route to fabricating functional UV-blocking cellulose/lignin composite films through a facile dissolution-regeneration process using these biomass residues was proposed. Three representative lignin residues, i.e., aspen and poplar wood lignin, and corn stover (CS) lignin were assessed for their feasibility for the film fabrication. The UV-blocking performance of the composite films were comparatively investigated. Results showed that all these three lignin residues could enhance the UV-blocking property of the composite films, corresponding to the reduction in the optical energy band gap from 4.31 to 3.72 eV, while poplar lignin had a considerable content of chromophores and showed the best UV-blocking enhancement among these three assessing lignins. The enhancement of UV-blocking property was achieved without compromising the visible-light transparency, mechanical strength and thermal stability of the composite films even at 4% lignin loading. This work showed the high promise of integrating biomass residue conversion into lignocellulose biorefinery for a multi-production purpose.
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http://dx.doi.org/10.3390/molecules27051637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911642PMC
March 2022

Quality Evaluation of Decoction Pieces of Gardeniae Fructus Based on Qualitative Analysis of the HPLC Fingerprint and Triple-Q-TOF-MS/MS Combined with Quantitative Analysis of 12 Representative Components.

J Anal Methods Chem 2022 26;2022:2219932. Epub 2022 Feb 26.

School of Pharmacy, Nantong University, Nantong 226019, Jiangsu, China.

In this study, quality evaluation (QE) of 40 batches of decoction pieces of Gardeniae Fructus (GF) produced by different manufacturers of herbal pieces was performed by qualitative analysis of the HPLC fingerprint and ultra-fast liquid chromatography (UFLC)-triple-Q-TOF-MS/MS combined with quantitative analysis of multiple components, which we established previously for QE of traditional medicine. First, HPLC fingerprints of 40 samples were determined, and the common peaks in the reference fingerprint were assigned. Second, the components of the common peaks in the HPLC fingerprints were identified by UFLC-triple-Q-TOF-MS/MS. Finally, the contents of the components confirmed by reference substances were measured. The results showed that there were 28 common peaks in the HPLC fingerprints of 40 samples. The components of these 28 common peaks were identified as 13 iridoids, 4 crocins, 7 monocyclic monoterpenoids, 3 organic acids, and 1 flavonoid. Of these, a total of 12 components, including 7 iridoids of geniposide, shanzhiside, geniposidic acid, deacetyl asperulosidic acid methyl ester, gardenoside, scandoside methyl ester, and genipin gentiobioside, 2 crocins such as crocin I and crocin II, 1 monocyclic monoterpenoid of jasminoside B, 1 organic acid of chlorogenic acid, and 1 flavonoid of rutin, were unambiguously identified by comparison with reference substances. There were certain differences in the contents of these 12 components among 40 samples. The geniposide content ranged from 37.917 to 72.216 mg/g, and the total content of the 7 iridoids ranged from 59.931 to 94.314 mg/g.
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http://dx.doi.org/10.1155/2022/2219932DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8898142PMC
February 2022

Generation of immunodeficient pig with hereditary tyrosinemia type 1 and their preliminary application for humanized liver.

Cell Biosci 2022 Mar 7;12(1):26. Epub 2022 Mar 7.

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

Background: Mice with humanized livers are important models to study drug toxicology testing, development of hepatitis virus treatments, and hepatocyte transplantation therapy. However, the huge difference between mouse and human in size and anatomy limited the application of humanized mice in investigating human diseases. Therefore, it is urgent to construct humanized livers in pigs to precisely investigate hepatocyte regeneration and human hepatocyte therapy. CRISPR/Cas9 system and somatic cell cloning technology were used to generate two pig models with FAH deficiency and exhibiting severe immunodeficiency (FAH/RAG1 and FAH/RAG1/IL2RG deficiency). Human primary hepatocytes were then successfully transplanted into the FG pig model and constructed two pigs with human liver.

Results: The constructed FAH/RAG1/IL2RG triple-knockout pig models were characterized by chronic liver injury and severe immunodeficiency. Importantly, the FG pigs transplanted with primary human hepatocytes produced human albumin in a time dependent manner as early as 1 week after transplantation. Furthermore, the colonization of human hepatocytes was confirmed by immunochemistry staining.

Conclusions: We successfully generated pig models with severe immunodeficiency that could construct human liver tissues.
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http://dx.doi.org/10.1186/s13578-022-00760-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8900390PMC
March 2022

Near-Infrared Spectral Characteristic Extraction and Qualitative Analysis Method for Complex Multi-Component Mixtures Based on TRPCA-SVM.

Sensors (Basel) 2022 Feb 20;22(4). Epub 2022 Feb 20.

School of Automation & Information Engineering, Sichuan University of Science & Engineering, No. 1 Baita Road, Yibin 644000, China.

Quality identification of multi-component mixtures is essential for production process control. Artificial sensory evaluation is a conventional quality evaluation method of multi-component mixture, which is easily affected by human subjective factors, and its results are inaccurate and unstable. This study developed a near-infrared (NIR) spectral characteristic extraction method based on a three-dimensional analysis space and establishes a high-accuracy qualitative identification model. First, the Norris derivative filtering algorithm was used in the pre-processing of the NIR spectrum to obtain a smooth main absorption peak. Then, the third-order tensor robust principal component analysis (TRPCA) algorithm was used for characteristic extraction, which effectively reduced the dimensionality of the raw NIR spectral data. Finally, on this basis, a qualitative identification model based on support vector machines (SVM) was constructed, and the classification accuracy reached 98.94%. Therefore, it is possible to develop a non-destructive, rapid qualitative detection system based on NIR spectroscopy to mine the subtle differences between classes and to use low-dimensional characteristic wavebands to detect the quality of complex multi-component mixtures. This method can be a key component of automatic quality control in the production of multi-component products.
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http://dx.doi.org/10.3390/s22041654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880016PMC
February 2022

An alternative downstream translation start site in the non-TIR adaptor Scimp enables selective amplification of CpG DNA responses in mouse macrophages.

Immunol Cell Biol 2022 Apr 22;100(4):267-284. Epub 2022 Mar 22.

Institute for Molecular Bioscience (IMB), IMB Centre for Inflammation and Disease Research, and Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD, Australia.

Toll-like receptor (TLR) signaling relies on Toll/interleukin-1 receptor homology (TIR) domain-containing adaptor proteins that recruit downstream signaling molecules to generate tailored immune responses. In addition, the palmitoylated transmembrane adaptor protein family member Scimp acts as a non-TIR-containing adaptor protein in macrophages, scaffolding the Src family kinase Lyn to enable TLR phosphorylation and proinflammatory signaling responses. Here we report the existence of a smaller, naturally occurring translational variant of Scimp (Scimp TV1), which is generated through leaky scanning and translation at a downstream methionine. Scimp TV1 also scaffolds Lyn, but in contrast to full-length Scimp, it is basally rather than lipopolysaccharide (LPS)-inducibly phosphorylated. Macrophages from mice that selectively express Scimp TV1, but not full-length Scimp, have impaired sustained LPS-inducible cytokine responses. Furthermore, in granulocyte macrophage colony-stimulating factor-derived myeloid cells that express high levels of Scimp, selective overexpression of Scimp TV1 enhances CpG DNA-inducible cytokine production. Unlike full-length Scimp that localizes to the cell surface and filopodia, Scimp TV1 accumulates in intracellular compartments, particularly the Golgi. Moreover, this variant of Scimp is not inducibly phosphorylated in response to CpG DNA, suggesting that it may act via an indirect mechanism to enhance TLR9 responses. Our findings thus reveal the use of alternative translation start sites as a previously unrecognized mechanism for diversifying TLR responses in the innate immune system.
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http://dx.doi.org/10.1111/imcb.12540DOI Listing
April 2022

A Sensitive Monoclonal-Antibody-Based ELISA for Forchlorfenuron Residue Analysis in Food Samples.

Biosensors (Basel) 2022 Jan 28;12(2). Epub 2022 Jan 28.

Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.

In this study, forchlorfenuron (CPPU) was coupled with succinic anhydride to yield a CPPU hapten (CPPU-COOH), and a high-affinity monoclonal antibody (mAb) that can specifically recognize CPPU was produced. Using this mAb as a recognition reagent, a sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) for CPPU was optimized, which exhibits an IC of 1.04 ng/mL, a limit of detection of 0.16 ng/mL, and a linear range of 0.31-3.43 ng/mL for CPPU. Cross-reactivity percentages with six analogues were all below 6%. The average recovery rates for cucumber and orange samples were from 85.23% to 119.14%. The analysis results of this icELISA showed good consistency with those from liquid chromatography mass spectrometry. These results suggest that the proposed icELISA provides a sensitive, specific, and reliable strategy for CPPU detection in food samples.
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http://dx.doi.org/10.3390/bios12020078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869720PMC
January 2022

[Research on the clinical application of drainage tube after the operation of percutaneous endoscopic lumbar discectomy].

Zhongguo Gu Shang 2022 Feb;35(2):122-7

Hubei 672 Orthopedic Hospital of Integrated Traditional Chinese and Western Medicine, Wuhan 430079, Hubei, China.

Objective: To investigate the clinical significance and related factors of drainage tube after percutaneous endoscopic lumbar discectomy(PELD).

Methods: The clinical data of 151 patients with lumbar disc herniation who underwent PELD from January 2019 to September 2019 was retrospectively analyzed. According to whether the drainage tube was used after operation, the patients were divided into drainage tube group and non drainage tube group. The placement time and total drainage volume were recorded. The characteristics of patients, such as age, gender, body mass index, lumbar disc herniation segment, smoking history, basic diseases and whether taking anticoagulants, were analyzed by single factor and multiple factor.

Results: Drainage tubes were used in 32 patients after PELD. There were statistical differences in visual analogue scale(VAS) and Japanese Orthopaedic Assiciation(JOA) scores between postoperative and preoperative of that in two groups(<0.05). There were statistical differences in VAS and JOA scores at discharge between two groups(<0.05), while there were no statistical differences at other time points(>0.05). Univariate analysis showed that age, basic diseases and whether taking anticoagulants were related to the use of drainage tube, but gender, body mass index, lumbar disc herniation segment and smoking history were not significantly related to the use of drainage tube. Multivariate analysis showed that elderly patients, complicated with hypertension and diabetes, taking anticoagulants were related to the use of drainage tube.

Conclusion: The use of drainage tube after percutaneous endoscopic lumbar discectomy can improve the symptoms of lumbar and leg pain in early stage. For elderly patients with hypertension, diabetes and taking anticoagulants drugs, drainage tube can be considered after transforaminal endoscopy.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2022.02.006DOI Listing
February 2022

Effective and repeatable chromatographic separation of 5 nucleotides in infant formula milk powder by ion-pair high-performance liquid chromatography-ultraviolet.

J Dairy Sci 2022 Apr 16;105(4):2840-2848. Epub 2022 Feb 16.

Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

A robust method using HPLC-UV was developed to improve the accuracy and repeatability of a quantitative method to detect 5 nucleotides (cytidine-monophosphate, uridine monophosphate, adenosine monophosphate, guanine monophosphate, and inosine monophosphate) in infant formulas. The results showed that efficient separation could not be achieved without strict conditions. The proposed method displayed a strong linear relationship (R > 0.9999) of single nucleotide in infant formula milk powder in the range of 10 to 1,000 mg/kg, a steady recovery (80.0% ∼110.0%) with relative standard deviation from 0.5% to 3.5%, under strict conditions of hydrophilic C column with di-isopropyl at 62.5 ± 2.5°C (± standard deviation), 0.65 ± 0.1 mg/mL tetrabutylammonium bisulfate, and mobile phase of pH of 2.75 ± 0.02. By applying this method on a series of milk products in the Chinese market, we found a few of them exceeded the legal limits of nucleotides.
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http://dx.doi.org/10.3168/jds.2021-21133DOI Listing
April 2022

Survival outcomes of radiofrequency ablation compared with surgery in patients with early-stage primary non-small-cell lung cancer: A meta-analysis.

Respir Investig 2022 May 13;60(3):337-344. Epub 2022 Feb 13.

Department of Respiratory and Critical Care Medicine, The People's Hospital of Leshan, Leshan, 614000, Sichuan, PR China. Electronic address:

Background: This study compared the overall survival (OS) of patients with early-stage primary non-small-cell lung cancer (NSCLC) treated with radiofrequency ablation (RFA) versus surgery.

Methods: A systematic search was performed in MEDLINE, Embase, Cochrane Central Register, and all available Chinese databases to identify relevant publications from inception to April 2019. This meta-analysis compared hazard ratios (HRs) for OS. A multivariate fixed effects model was used to perform a meta-analysis to compare survival between treatments.

Results: Six retrospective studies were included in the quantitative synthesis. Compared with surgery, RFA was associated with a similar long-term OS. The HRs and 95% confidence intervals (CIs) for 2-, 3- and 5-year OS were 1.74 [0.82, 3.71], 1.15 [0.65, 2.02] and 2.69 [0.41, 17.47], respectively, while those of the pooled data were 1.47 [0.94, 2.32] in patients with early-stage primary NSCLC.

Conclusions: RFA did not differ significantly from surgery in terms of the 5-year OS in patients with early-stage primary NSCLC. Randomized, controlled clinical trials are warranted to compare these two treatments.
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http://dx.doi.org/10.1016/j.resinv.2022.01.002DOI Listing
May 2022

Preparation of an Immunoaffinity Column Based on Bispecific Monoclonal Antibody for Aflatoxin B and Ochratoxin A Detection Combined with ic-ELISA.

Foods 2022 Jan 25;11(3). Epub 2022 Jan 25.

Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.

A novel and efficient immunoaffinity column (IAC) based on bispecific monoclonal antibody (BsMAb) recognizing aflatoxin B (AFB) and ochratoxin A (OTA) was prepared and applied in simultaneous extraction of AFB and OTA from food samples and detection of AFB/OTA combined with ic-ELISA (indirect competitive ELISA). Two deficient cell lines, hypoxanthine guanine phosphoribosyl-transferase (HGPRT) deficient anti-AFB hybridoma cell line and thymidine kinase (TK) deficient anti-OTA hybridoma cell line, were fused to generate a hybrid-hybridoma producing BsMAb against AFB and OTA. The subtype of the BsMAb was IgG via mouse antibody isotyping kit test. The purity and molecular weight of BsMAb were confirmed by SDS-PAGE method. The cross-reaction rate with AFB was 37%, with AFG 15%, with AFM 48%, with AFM 10%, and with OTB 36%. Negligible cross-reaction was observed with other tested compounds. The affinity constant (Ka) was determined by ELISA. The Ka (AFB) and Ka (OTA) was 2.43 × 10 L/mol and 1.57 × 10 L/mol, respectively. Then the anti-AFB/OTA BsMAb was coupled with CNBr-Sepharose, and an AFB/OTA IAC was prepared. The coupling time and elution conditions of IAC were optimized. The coupling time was 1 h with 90% coupling rate, the eluent was methanol-water (60:40, :, pH 2.3) containing 1 mol/L NaCl, and the eluent volume was 4 mL. The column capacities of AFB and OTA were 165.0 ng and 171.3 ng, respectively. After seven times of repeated use, the preservation rates of column capacity for AFB and OTA were 69.3% and 68.0%, respectively. The ic-ELISA for AFB and OTA were applied combined with IAC. The IC (50% inhibiting concentration) of AFB was 0.027 ng/mL, the limit of detection (LOD) was 0.004 ng/mL (0.032 µg/kg), and the linear range was 0.006 ng/mL~0.119 ng/mL. The IC of OTA was 0.878 ng/mL, the LOD was 0.126 ng/mL (1.008 µg/kg), and the linear range was 0.259 ng/mL~6.178 ng/mL. Under optimum conditions, corn and wheat samples were pretreated with AFB-OTA IAC. The recovery rates of AFB and OTA were 95.4%~105.0% with ic-ELISA, and the correlations between the detection results and LC-MS were above 0.9. The developed IAC combined with ic-ELISA is reliable and could be applied to the detection of AFB and OTA in grains.
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http://dx.doi.org/10.3390/foods11030335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8833996PMC
January 2022

Phenylarsonics in concentrated animal feeding operations: Fate, associated risk, and treatment approaches.

J Hazard Mater 2022 05 1;430:128394. Epub 2022 Feb 1.

College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China. Electronic address:

Phenylarsonics are present as additives in animal feed in some countries. As only a small fraction of these additives is metabolized in animals, they mostly end up in the environment. A comprehensive investigation of the fate of these additives is crucial for evaluating their risks. This review aims to provide a clear understanding of the transformation mechanism of phenylarsonics in vivo and in vitro and to evaluate their fate and associated risks. Degradation of phenylarsonics releases toxic As species (mainly as inorganic arsenic (iAs)). Trivalent phenylarsonics are the metabolites or biotic degradation intermediates of phenylarsonics. The cleavage of As groups from trivalent phenylarsonics catalyzed by C-As lyase or other unknown pathways generates arsenite (As(III)). As(III) can be further oxidized to arsenate (As(V)) and methylated to methyl-arsenic species. The half-lives associated with abiotic degradation of phenylarsonics ranged from a few minutes to tens of hours, while those associated with biotic degradation ranged from several days to hundreds of days. Abiotic degradation resulted in a higher yield of iAs than biotic degradation. The use of phenylarsonics led to elevated total As and iAs levels in animal products and environmental matrices, resulting in As exposure risk to humans. The oxidation of phenylarsonics to As(V) facilitated the sorptive removal of As, which provides a general approach for treating these compounds. This review provides solid evidence that the use of phenylarsonics has adverse effects on both human health and environmental safety, and therefore, supports their withdrawal from the global market.
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http://dx.doi.org/10.1016/j.jhazmat.2022.128394DOI Listing
May 2022

Halogenated BODIPY photosensitizers: Photophysical processes for generation of excited triplet state, excited singlet state and singlet oxygen.

Spectrochim Acta A Mol Biomol Spectrosc 2022 May 31;272:120965. Epub 2022 Jan 31.

Department of Chemical Engineering, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei Province 066004, China.

We have systematically examined the formation of singlet oxygen O(Δ), the excited triplet state (T), and excited singlet state (S) for halogenated BODIPY photosensitizers (halogen = Cl, Br, and I) in eight solvents to understand how halogen atoms and solvent affect these properties. The phosphorescence spectra and lifetimes of singlet oxygen generated by these halogenated BODIPYs have been measured by steady state/time resolved NIR emission, while the formation quantum yield of singlet oxygen (Φ) has been determined by chemical method using diphenylisobenzofuran (DPBF) as the trapping agent. The formation quantum yield Φ of singlet oxygen can be as high as 0.96 for iodinated BODIPY and 0.71 for brominated BODIPY. The triplet state T absorption spectra of brominated and iodinated BODIPYs have been recorded by laser flash photolysis method, in which T shows high formation efficiency and long lifetime. The formation and decay of excited singlet state S of four BODIPYs have been measured by ground state (S) absorption and steady state/time resolved fluorescence. The results show that larger halogen atoms on BODIPY core lead to smaller fluorescence quantum yield, shorter fluorescence lifetime and higher singlet oxygen formation quantum yield due to heavy atom effect that promotes the formation of triplet state. On the other hand, higher solvent polarity causes lower singlet oxygen formation quantum yield, smaller fluorescence quantum yield, and shorter fluorescence lifetime. This solvent effect is explained by the presence of photoinduced charge transfer (ICT) process from halogen atoms to BODIPY. The ICT efficiency has been estimated and the results are agreed with ICT theory. ICT process in halogenated BODIPYs has never been revealed in literature. HOMO/LUMO obtained from DFT calculation also supports the presence of ICT. The involvement of ICT in the photosensitizing process of halogenated BODIPYs provides new insights for designing BODIPY photosensitizers for photodynamic therapy of tumor.
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http://dx.doi.org/10.1016/j.saa.2022.120965DOI Listing
May 2022

RNF2 ablation reprograms the tumor-immune microenvironment and stimulates durable NK and CD4 T-cell-dependent antitumor immunity.

Nat Cancer 2021 10 22;2(10):1018-1038. Epub 2021 Oct 22.

Department of Radiation Oncology, University of Alabama at Birmingham School of Medicine, Hazelrig Salter Radiation Oncology Center, Birmingham, AL, USA.

Expanding the utility of immune-based cancer treatments is a clinical challenge due to tumor-intrinsic factors that suppress the immune response. Here we report the identification of tumoral ring finger protein 2 (RNF2), the core subunit of polycomb repressor complex 1, as a negative regulator of antitumor immunity in various human cancers, including breast cancer. In syngeneic murine models of triple-negative breast cancer, we found that deleting genes encoding the polycomb repressor complex 1 subunits Rnf2, BMI1 proto-oncogene, polycomb ring finger (Bmi1), or the downstream effector of Rnf2, remodeling and spacing factor 1 (Rsf1), was sufficient by itself to induce durable tumor rejection and establish immune memory by enhancing infiltration and activation of natural killer and CD4 T cells, but not CD8 T cells, into the tumor and enabled their cooperativity. These findings uncover an epigenetic reprogramming of the tumor-immune microenvironment, which fosters durable antitumor immunity and memory.
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http://dx.doi.org/10.1038/s43018-021-00263-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8809507PMC
October 2021

Benzotriazole Ultraviolet Stabilizers Promote Breast Cancer Cell Proliferation via Activating Estrogen-Related Receptors α and γ at Human-Relevant Levels.

Environ Sci Technol 2022 02 31;56(4):2466-2475. Epub 2022 Jan 31.

College of Resources and Environment, Hunan Agricultural University, 1 Nongda Road, Furong District, Changsha 410128, China.

Benzotriazole ultraviolet stabilizers (BUVSs) are ubiquitous emerging pollutants that have been reported to show estrogenic disruption effects through interaction with the classic estrogen receptors (ERs) in the fashion of low activity. The present study aims at revealing the potential disruption mechanism via estrogen-related receptors α and γ (ERRα and ERRγ) pathways. By the competitive binding assay, we first found that BUVSs bond to ERRγ ligand binding domain (ERRγ-LBD) with ranging from 0.66 to 19.27 μM. According to the results of reporter gene assays, the transcriptional activities of ERRα and ERRγ were promoted by most tested BUVSs with the lowest observed effective concentrations (LOEC) from 10 to 100 nM, which are in the range of human exposure levels. At 1 μM, most tested BUVSs showed higher agonistic activity toward ERRγ than ERRα. The most effective two BUVSs promoted the MCF-7 proliferation dependent on ERRα and ERRγ with a LOEC of 100 nM. The molecular dynamics simulation showed that most studied BUVSs had lower binding free energy with ERRγ than with ERRα. The structure-activity relationship analysis revealed that molecular polarizability, electron-donating ability, ionization potential, and softness were the main structural factors impacting the binding of BUVSs with ERRγ. Overall, our results provide novel insights into the estrogenic disruption effects of BUVSs.
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http://dx.doi.org/10.1021/acs.est.1c03446DOI Listing
February 2022

Silicon fertilizers, humic acid and their impact on physicochemical properties, availability and distribution of heavy metals in soil and soil aggregates.

Sci Total Environ 2022 May 29;822:153483. Epub 2022 Jan 29.

Hunan International Scientific and Technological Cooperation Center for Agricultural Pollution Remediation and Wetland Protection, College of Resources and Environment, Hunan Agricultural University, Changsha, Hunan 410028, China.

It has been confirmed that silicon (Si) fertilizer and humic acid (HA) could effectively decrease the heavy metals in soil. Nonetheless, the impact of these additives on soil aggregate characteristics was ignored. Therefore, the effects of Si fertilizer, HA, and their combinations on the physicochemical characteristics, availability of heavy metals (Cu, Cd, Pb, Zn), and fraction changes in soils and soil aggregates were investigated in this research. The results showed that Si fertilizer and HA significantly modified soil properties such as soil pH, electrical conductivity total organic carbon, water-soluble organic carbon, and nitrate‑nitrogen. HA and Si-HA (SHA) supplementation significantly decreased the availability of Cu, Cd, Pd, and Zn. Besides, there was no significant difference in physicochemical properties between soil and soil aggregates. The availability of Cu, Cd, Pd, and Zn in soil aggregates could be significantly inhibited by the addition of HA and SHA, and the content in microaggregates was greater than that in macroaggregates. After the addition of the three additives, the main fractions of heavy metals in different particle sizes were changed and eventually transformed to the residue state. These results indicated that Si fertilizer, HA, and SHA were influential in physicochemical properties and metal availability in soil aggregates. Therefore, it is of great scientific significance to study the impact of heavy metal pollution on the ecological environment in different aggregates, which will provide reference data for future sustainable management of heavy-metal polluted soils.
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http://dx.doi.org/10.1016/j.scitotenv.2022.153483DOI Listing
May 2022

Efficient removal of pefloxacin from aqueous solution by acid-alkali modified sludge-based biochar: adsorption kinetics, isotherm, thermodynamics, and mechanism.

Environ Sci Pollut Res Int 2022 Jan 29. Epub 2022 Jan 29.

Hunan International Scientific and Technological Cooperation Base of Agricultural Typical Pollution Remediation and Wetland Protection, College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, China.

In this paper, one kind of acid-alkali modified sludge-based biochar (ASBC) was synthesized, characterized, and employed as adsorbent for the removal of pefloxacin. The characterization results showed that the specific surface area (SSA) of ASBC (53.381 m/g) was significantly higher than that of SBC (24.411 m/g). ASBC had a rougher surface, larger particle distribution, lower zero point charge, and richer functional groups (e.g., C-O and O-H) than SBC. The adsorption capacity of ASBC was 1.82 times than that of SBC. After 8 adsorption cycles in reuse experiment, the adsorption capacity of ASBC for pefloxacin still reached 144.08 mg/L, indicating that ASBC has good reusability. Static experiments showed that the optimal pH value was 6.0 in the adsorption of pefloxacin on SBC and ASBC. The result of adsorption kinetics indicated that the pseudo-second-order model could describe well the adsorption process. The Freundlich model was better than the Langmuir model to describe the adsorption of pefloxacin by ASBC, indicating that the adsorption process was mainly multilayer adsorption. Thermodynamic result showed that the adsorption of pefloxacin by ASBC was spontaneous and endothermic. The removal mechanism of pefloxacin by ASBC is mainly the substitution reaction and π-π EDA interaction. In summary, acid-alkali modified biochar is an effective adsorbent for pefloxacin in aqueous solution, and has great application prospects.
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http://dx.doi.org/10.1007/s11356-021-18220-9DOI Listing
January 2022
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