Publications by authors named "Lin Liu"

2,880 Publications

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Dark-light cycle disrupts bone metabolism and suppresses joint deterioration in osteoarthritic rats.

Arthritis Res Ther 2022 Jun 28;24(1):158. Epub 2022 Jun 28.

Heilongjiang Key Laboratory of Animal Disease Pathogenesis and Comparative Medicine, College of Veterinary Medicine, Northeast Agriculture University, 600, Changjiang Road, Harbin, 150030, China.

Background: Light alteration affects the internal environment and metabolic homeostasis of the body through circadian rhythm disorders (CRD). CRD is one of the factors that induce and accelerate osteoarthritis (OA). Therefore, the aim of this study was to evaluate the effects of continuous dark-light (DL) cycle on joint inflammation, bone structure, and metabolism in normal and OA Sprague-Dawley (SD) rats.

Methods: Interleukin (IL)-1β, IL-6, inducible nitric oxide synthase (iNOS), and tumor necrosis factor (TNF)-α were used to evaluate the systemic inflammation in rats. The pathological changes and inflammatory reactions of the cartilage and synovium of the knee joint in rats were evaluated by Safranin O-fast green and immunological staining. Bone turnover was assessed by histomorphometry and μCT scanning, as well as bone metabolism markers and proteins. The expression changes of clock proteins BMAL1, NR1D1, PER3, and CRY1 in representative tissues were detected by western blotting.

Results: DL cycle significantly inhibited body weight gain in normal and OA rats. The levels of proinflammatory factors in the peripheral blood circulation and degradation enzymes in the cartilage were significantly decreased in OA+DL rats. DL cycle significantly destroyed the structure of subchondral bone in hindlimbs of OA rats and reduced trabecular bone numbers. The decrease of bone mineral density (BMD), percent bone volume with respect to total bone volume (BV/TV), trabecular number (TB.N), osteoclast number, and mineralization could also be found. The ratio of the receptor activator of nuclear factor-kappa B ligand/osteoprotegerin (RANKL/OPG) in the bone marrow of OA rats was markedly increased under DL, along with the activation of the mononuclear/phagocyte system. The expression of representative clock proteins and genes BMAL1, PER3, and CRY1 were markedly changed in the tissues of OA+DL rats.

Conclusions: These results suggested that DL cycle dampened the arthritis and promoted bone resorption and bone mass loss. DL cycle affects bone turnover by regulating osteoclast production in osteoarthritic rats.
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http://dx.doi.org/10.1186/s13075-022-02832-8DOI Listing
June 2022

Effect of intracranial venous collaterals on neurological outcomes in cerebral venous thrombosis.

J Clin Neurosci 2022 Jun 25;102:95-100. Epub 2022 Jun 25.

Department of Neurology, The Second Hospital of Hebei Medical University, 215 West, Heping Road, Shijiazhuang, Hebei 050000, China; Neuroscience Research Center, Medicine and Health Institute, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang, Hebei 050000, China. Electronic address:

This study identifies predictors of favourable intracranial venous collaterals and the effect of intracranial venous collaterals on outcomes and recanalization in patients with cerebral venous thrombosis (CVT). Data of 61 patients with CVT were retrospectively reviewed. Venous collateralization was defined as expanded cortical vein formation through different drainage pathways. Recanalization grades were classified into complete or partial recanalization based on images obtained during hospitalisation and follow-up. Independent predictors of collateral formation and poor prognosis were investigated via univariate and binary logistic regression analyses. The effects of different intracranial venous collaterals on recanalization in patients with CVT were assessed. A risk prediction nomogram for prognosis was constructed. Age ≤ 35 years (odds ratio (OR) = 7.067; 95% confidence interval (CI) = 1.776-28.277; P = 0.006) and male sex (OR = 5.490; 95% CI = 1.205-25.004; P = 0.028) were independent predictors of favourable venous collaterals. Venous collaterals were associated with early recanalization (P = 0.017) and not with long-term recanalization (P = 0.252). Male sex (OR = 0.047; 95% CI = 0.003-0.651; P = 0.023), subacute onset (OR = 0.026; 95% CI = 0.002-0.367; P = 0.007), and good collateral grade (OR = 0.168; 95% CI = 0.029-0.985; P = 0.048) were independent factors of favourable neurological outcomes at discharge. Haemorrhage on computed tomography at admission (OR = 10.868; 95% CI = 2.082-56.733; P = 0.005) was inversely correlated with prognosis. These findings suggested that male patients under 35 years of age are more likely to have favourable venous collaterals and good outcomes. Venous collaterals are significantly associated with early recanalization. These findings highlight the importance of venous collateral evaluation in patients with CVT.
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http://dx.doi.org/10.1016/j.jocn.2022.06.009DOI Listing
June 2022

A Post-GWAS Functional Analysis Confirming Effects of Three BTA13 Genes , , and on Dairy Cattle Reproduction.

Front Genet 2022 8;13:882951. Epub 2022 Jun 8.

Key Laboratory of Animal Genetics, Breeding and Reproduction, MARA, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.

In our previous GWAS of Chinese and Nordic dairy cattle, genes , and locating on BTA 13 were suggested as candidate genes for reproduction. In this study, validation of these associations was performed in an independent population with records of nine reproductive traits. More importantly, functions of these genes in the reproductive process were verified by employing the expression data of ovarian follicles. The potential variants within the three genes were firstly detected in 68 Chinese Holstein bulls, and then screened in 1,588 Chinese Holstein cows using the KASP (Kompetitive allele-specific PCR) method. There were nine variants with polymorphisms in , five in and four in , respectively, of which one SNP was in the upstream regulatory region, two in exon region, four in downstream regulatory region, and 11 SNPs in intronic regions. Amongst the 18 variants, g.33267056T/G in explained the largest phenotypic variance for age at first calving (0.011%), interval from first to last insemination (0.004%), and calving ease (0.002%), while g.32751518G/A in contributed the most to stillbirth in heifers (0.038%). Two haplotype blocks were constructed for while one each for and , which were significantly associated with five reproductive traits, including age at the first service, age at the first calving, calving ease in heifers and cows, and the interval from calving to the first insemination. We then studied the profile of gene expression in granulosa cells isolated from four developmental stages of ovarian follicles from eight dairy cows. All three genes were differentially expressed between ovarian follicles with different sizes ( < 0.05), indicating their potential roles in the reproductive process of dairy cows. This study successfully demonstrated the associations of three BTA 13 genes , and with reproduction and further examined their expression levels in ovarian follicles directly. These findings can be beneficial for the ongoing genomic selection program for reproductive traits which have long been considered as traits that are difficult to achieve genetic improvement due to the lack of efficient genetic markers.
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http://dx.doi.org/10.3389/fgene.2022.882951DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9216173PMC
June 2022

Exploring the Scientific Rationality of the Phenomenon of "Different Dosage Forms of the Same Prescription" of Chinese Proprietary Medicine Based on Biopharmaceutical Properties of Powder and Pill of Chuanxiong Chatiao Prescription.

Front Pharmacol 2022 9;13:893552. Epub 2022 Jun 9.

Pharmacy College, Shaanxi University of Chinese Medicine, Xianyang, China.

The 2020 edition of the Pharmacopoeia of the People's Republic of China (Chinese Pharmacopoeia 2020 edition) has 255 Chinese prescriptions with different dosage forms, accounting for 21.09% of the total prescriptions (1,209) in Chinese Pharmacopoeia 2020 edition. However, the scientific rationality of the phenomenon of "Different Dosage Forms of the Same Prescription" of Chinese proprietary medicine has been less explored. Based on the dosage form theory of "components in pills release slowly and take effect in slow-acting manner, while in powders release quickly and take effect in fast-acting way," we provided the dissolution experiment and pharmacokinetics of Chuanxiong Chatiao powders and pills in order to rationalize the phenomenon of "Different Dosage Forms of the Same Prescription" of Chuanxiong Chatiao prescription. Chuanxiong Chatiao powders and pills were prepared in the laboratory referring to the preparation methods in the Chinese Pharmacopoeia 2020 edition, and the contents of tetramethylpyrazine, ferulic acid, nodakenin, and isoimperatorin were determined by the external standard method. We measured the dissolution of four analytes of Chuanxiong Chatiao powders and pills according to the second method for dissolution determination (paddle method) in the Chinese Pharmacopoeia 2020 edition, and their corresponding contents in each sampling point were determined by LC-MS/MS. We also provided a pharmacokinetic study of Chuanxiong Chatiao powders and pills. Six female domestic rabbits were divided into two groups (powder and pill groups) and given Chuanxiong Chatiao powders and pills (9.85 g/kg) by surgical administration separately. Blood samples were collected at 5, 15, 30, 45, 60, 90, 120, 150, 180, 240, 360, 480, 720, and 1,440 min after drug administration to measure the plasma concentration of the four analytes by LC-MS/MS. The results of dissolution experiment showed that the dissolution rate of four analytes in the powder group was greater than that of the pill group. However, the solubilities of tetramethylpyrazine and isoimperatorin were very low in the powder and pill, which may be related to their low solubility properties. The results of the pharmacokinetic study of Chuanxiong Chatiao powders and pills showed that (h) of ferulic acid and nodakenin in the powder group was 0.420 and 0.053 times that of the pill group and (h) of ferulic acid, nodakenin, and isoimperatorin of the powder group was 0.910, 0.262, and 0.661 times that of the pill group, respectively. The dissolution rate and pharmacokinetic parameters of four analytes in CXCTF could partly explain the scientific rationality of the classic theory of ", " as in Chinese, which is helpful for providing a basis for the comparison of subsequent dosage forms. The results of our studies also suggest the complexity of the design of dosage forms of Chinese proprietary medicines and imply that we should pay more attention to the scientific rationality of the phenomenon of "Different Dosage Forms of the Same Prescription."
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http://dx.doi.org/10.3389/fphar.2022.893552DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218571PMC
June 2022

A novel cold-active GH8 xylanase from cellulolytic myxobacterium and its application in food industry.

Food Chem 2022 Nov 11;393:133463. Epub 2022 Jun 11.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Biological Interactions and Crop Health, Nanjing Agricultural University, Nanjing 210095, China.

Although xylanase have a wide range of applications, cold-active xylanases have received less attention. In this study, a novel glycoside hydrolase family 8 (GH8) xylanase from Sorangium cellulosum with high activity at low temperatures was identified. The recombinant xylanase (XynSc8) was most active at 50 °C, demonstrating 20% of its maximum activity and strict substrate specificity towards beechwood and corncob xylan at 4 °C with V values of 968.65 and 1521.13 μmol/mg/min, respectively. Mesophilic XynSc8 was active at a broad range of pH and hydrolyzed beechwood and corncob xylan into xylooligosaccharides (XOS) with degree of polymerization greater than 3. Moreover, incorporation of XynSc8 (0.05-0.2 mg/kg flour) provided remarkable improvement (28-30%) in bread specific volume and textural characteristics of bread compared to commercial xylanase. This is the first report on a novel cold-adapted GH8 xylanase from myxobacteria, suggesting that XynSc8 may be a promising candidate suitable for bread making.
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http://dx.doi.org/10.1016/j.foodchem.2022.133463DOI Listing
November 2022

Cell surface glycan engineering reveals that matriglycan alone can recapitulate dystroglycan binding and function.

Nat Commun 2022 Jun 24;13(1):3617. Epub 2022 Jun 24.

Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.

α-Dystroglycan (α-DG) is uniquely modified on O-mannose sites by a repeating disaccharide (-Xylα1,3-GlcAβ1,3-) termed matriglycan, which is a receptor for laminin-G domain-containing proteins and employed by old-world arenaviruses for infection. Using chemoenzymatically synthesized matriglycans printed as a microarray, we demonstrate length-dependent binding to Laminin, Lassa virus GP1, and the clinically-important antibody IIH6. Utilizing an enzymatic engineering approach, an N-linked glycoprotein was converted into a IIH6-positive Laminin-binding glycoprotein. Engineering of the surface of cells deficient for either α-DG or O-mannosylation with matriglycans of sufficient length recovers infection with a Lassa-pseudovirus. Finally, free matriglycan in a dose and length dependent manner inhibits viral infection of wildtype cells. These results indicate that matriglycan alone is necessary and sufficient for IIH6 staining, Laminin and LASV GP1 binding, and Lassa-pseudovirus infection and support a model in which it is a tunable receptor for which increasing chain length enhances ligand-binding capacity.
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http://dx.doi.org/10.1038/s41467-022-31205-7DOI Listing
June 2022

Fluorescent Immunoassay with a Copper Polymer as the Signal Label for Catalytic Oxidation of -Phenylenediamine.

Molecules 2022 Jun 8;27(12). Epub 2022 Jun 8.

Henan Province of Key Laboratory of New Optoelectronic Functional Materials, Anyang Normal University, Anyang 455000, China.

This work suggested that Cu ion coordinated by the peptide with a histidine (His or H) residue in the first position from the free N-terminal reveals oxidase-mimicking activity. A biotinylated polymer was prepared by modifying His residues on the side chain amino groups of lysine residues (denoted as K) to chelate multiple Cu ions. The resulting biotin-poly-(K-Cu) polymer with multiple catalytic sites was employed as the signal label for immunoassay. Prostate specific antigen (PSA) was determined as the model target. The captured biotin-poly-(K-Cu) polymer could catalyze the oxidation of -phenylenediamine (OPD) to produce fluorescent 2,3-diaminophenazine (OPDox). The signal was proportional to PSA concentration from 0.01 to 2 ng/mL, and the detection limit was found to be eight pg/mL. The high sensitivity of the method enabled the assays of PSA in real serum samples. The work should be valuable for the design of novel biosensors for clinical diagnosis.
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http://dx.doi.org/10.3390/molecules27123675DOI Listing
June 2022

Clinical and laboratorial outcome of different age-onset systemic lupus erythematosus patients in Jiangsu, China: a multicentre retrospective study.

Sci Rep 2022 Jun 23;12(1):10683. Epub 2022 Jun 23.

Department of Rheumatology and Immunology, Affiliated Nanjing Drum Tower Hospital, Medical School of Nanjing University, 321 Zhongshan Road, Nanjing, 210008, China.

Studies on clinical features of systemic lupus erythematosus among different age-onset patients are lacking in China. This multicentre study aimed to systemically compare clinical manifestations, comorbidities, organ involvement, and laboratory findings among 797 Chinese juvenile-onset, adult-onset, and late-onset SLE (JSLE, ASLE, and LSLE) patients. They were classified into JSLE, ASLE, and LSLE groups if first diagnosed at < 18, 18-50, and > 50 years old, respectively. Chi-square test and analysis of variance were employed for categorical and continuous variables respectively. In younger-onset patients, the SLE Disease Activity Index 2000 score was significantly higher (JSLE vs. ASLE vs. LSLE = 17.43 ± 9.139 vs. 16.34 ± 8.163 vs. 14.08 ± 6.474, p = 0.031). Mucocutaneous symptoms (79.5% vs. 73.4% vs. 62.0%, p = 0.042), especially malar rash (76.1% vs. 66.1% vs. 53.5%, p = 0.011) occurred more frequently, and proteinuria rate was higher (54.5% vs. 56.3% vs. 36.6%, p = 0.007). In later-onset patients, cardiopulmonary involvement increased (11.4% vs. 24.3% vs. 29.6%, p = 0.012). In ASLE, hypoalbuminemia rate elevated (46.6% vs. 59.9% vs. 47.9%, p = 0.015). Our study demonstrated in a Chinese population that JSLE may be more active and suffer mucocutaneous disorders, while LSLE tended to suffer cardiopulmonary involvement at-onset. These findings may help identify treatment priorities when facing different age-onset SLE patients.
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http://dx.doi.org/10.1038/s41598-022-14840-4DOI Listing
June 2022

A Facile Method for Fabricating a Monolithic Mullite Fiber-Reinforced Alumina Aerogel with Excellent Mechanical and Thermal Properties.

Gels 2022 Jun 15;8(6). Epub 2022 Jun 15.

Shanghai Key Laboratory of Special Artificial Microstructure Materials and Technology, School of Physics Science and Engineering, Tongji University, Shanghai 200092, China.

Alumina aerogels are considered to have good application prospects in the high-temperature field. In this study, monolithic mullite fiber-reinforced alumina aerogels with excellent mechanical and thermal properties were synthesized via a facile method without the use of any chelating agents. This method successfully avoids the introduction of impurities during the use of catalysts and chelating agents while greatly reducing gelation time, and thus helps mullite fibers to uniformly disperse in the sol. The compressive stress at 80% strain of the obtained mullite fiber-reinforced alumina aerogels was as high as 16.04 MPa-426% higher than that of the alumina aerogel without the addition of mullite fibers. Regarding thermal properties, the shrinkage of the mullite fiber-reinforced alumina aerogels (AM) samples was less than 1% after heat treatment at 1300 °C for 2 h. Furthermore, the rear-surface temperature of the AM samples burned by a butane blow torch was only 68 °C. These outstanding properties make AM samples promising for application in thermal insulation materials in high-temperature fields such as aerospace and industrial thermal protection in the future.
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http://dx.doi.org/10.3390/gels8060380DOI Listing
June 2022

Calcium Homeostasis in Parkinson's Disease: From Pathology to Treatment.

Neurosci Bull 2022 Jun 21. Epub 2022 Jun 21.

Institute of Brain Science and Disease, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders, Qingdao University, Qingdao, 266071, China.

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http://dx.doi.org/10.1007/s12264-022-00899-6DOI Listing
June 2022

Increased hypertension risk for the elderly with high blood levels of strontium and lead.

Environ Geochem Health 2022 Jun 21. Epub 2022 Jun 21.

School of Public Health, Anhui Medical University, Hefei, Anhui, China.

Hypertension has long been recognized as the global health burden. Heavy metal pollution may be one of the environmental risk factors of hypertension. However, the association remains unclear. We studied the levels of aluminum (Al), vanadium (V), manganese (Mn), arsenic (As), selenium (Se), strontium (Sr), barium (Ba), titanium (Ti), lead (Pb) and cobalt (Co) in whole blood, and the relationship between trace element exposure and hypertension in the elderly community-based Chinese population. A total of 1013 participants from the west of Anhui Province in China were consecutively enrolled in this study in 2016. The general sociodemographic characteristics, lifestyles, disease history and physical examination information were collected by face-to-face survey and physical examination. The levels of ten trace elements were determined by inductively coupled plasma mass spectrometry (ICP-MS). Multivariable logistic regression model was used to assess the association of trace element exposure with the risk of hypertension. Results showed that the odds ratio of hypertension in the highest quartile was 1.811 (95% CI 1.175-2.790, P trend = 0.005) and 1.772 (95% CI 1.121-2.800, P trend = 0.022), respectively, after adjusting for potential confounders, as compared with the lowest quartile of blood Pb and Sr levels.
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http://dx.doi.org/10.1007/s10653-022-01317-6DOI Listing
June 2022

Exosomes From Cancer-Associated Mesenchymal Stem Cells Transmit TMBIM6 to Promote the Malignant Behavior of Hepatocellular Carcinoma Activating PI3K/AKT Pathway.

Front Oncol 2022 2;12:868726. Epub 2022 Jun 2.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Objective: Cancer-associated mesenchymal stem cells (MSCs) regulate the progression of cancers through exosome-delivered components, while few studies are conducted on hepatocellular carcinoma (HCC). This study aimed to evaluate the effect of exosomes from HCC-associated MSCs (HCC-MSCs) on HCC cellular functions and the potential regulatory mechanism.

Methods: HCC cells (Huh7 and PLC) were cultured normally or co-cultured with HCC-MSCs, HCC-MSCs plus GW4869, or HCC-MSC-derived exosomes; then mRNA sequencing and RT-qPCR validation were conducted. Subsequently, candidate genes were sorted out and modified in HCC cells. Next, TMBIM6-modified HCC-MSCs were used to treat HCC cells.

Results: Both HCC-MSCs and their derived exosomes promoted proliferation, invasion, sphere formation ability but suppressed apoptosis in HCC cells (all < 0.05); however, the effect of HCC-MSCs on these cellular functions was repressed by exosome inhibitor (GW4869). Subsequently, TMBIM6, EEF2, and PRDX1 were sorted out by mRNA sequencing and RT-qPCR validation as candidate genes implicated in the regulation of HCC cellular functions by HCC-MSC-derived exosomes. Among them, TMBIM6 had a potent effect (all < 0.05), while EEF2 and PRDX1 had less effect on regulating HCC cell viability and invasion. Next, direct silencing TMBIM6 repressed viability, sphere formation, invasion, epithelial-mesenchymal transition (EMT), and PI3K/AKT pathway but promoted apoptosis in HCC cells; however, overexpressing TMBIM6 showed the opposite effect. Furthermore, incubating with exosomes from TMBIM6-modified HCC-MSCs presented a similar effect as direct TMBIM6 modification in HCC cells.

Conclusion: HCC-MSC-derived exosomes transmit TMBIM6 to promote malignant behavior PI3K/AKT pathway in HCC.
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http://dx.doi.org/10.3389/fonc.2022.868726DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201337PMC
June 2022

Related factors associated with fear of hypoglycemia in parents of children and adolescents with type 1 diabetes - A systematic review.

J Pediatr Nurs 2022 Jun 15;66:125-135. Epub 2022 Jun 15.

School of Nursing, Yangzhou University, Yangzhou, China; Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, China. Electronic address:

Problem: Fear of hypoglycemia is a significant concern for parents of children/ adolescents with type 1 diabetes. Although some studies have explained the parental fear of hypoglycemia, the related factors were yet to be determined. This systematic review aims to identify the related factors of fear of hypoglycemia in the parents of children and adolescents with type 1 diabetes and provide a theoretical basis for further intervention.

Eligibility Criteria: PubMed, MEDLINE, EMBASE, Scopus, CINAHL, EBSCO, Web of Science, and Cochrane Library were systematically searched from 2010 to 2021. Studies evaluating the fear of hypoglycemia of parents and its associated factors were included.

Sample: Twenty-three observational articles met the criteria.

Results: Significant associations were found between fear of hypoglycemia and specific factors, including motherhood, nocturnal hypoglycemia, and the number of blood glucose monitoring. Psychological factors, including anxiety, depression, pediatric parenting stress, mindfulness, self-efficacy, quality of life, and sleep disorders, were conclusive and associations with parental fear of hypoglycemia.

Conclusions: Understanding parental fear of hypoglycemia can help parents prevent potential problems in diabetes management, thus promoting children's growth. According to current evidence, effective targeted interventions based on modifiable relevant factors can be developed to reduce the fear of hypoglycemia in parents while maintaining optimal blood glucose control in children/ adolescents.

Implications: Health professionals should pay more attention to the mental health of parents, and parents should be involved in the care plan and have the opportunity to discuss their fear of hypoglycemia in the most appropriate way to manage type 1 diabetes.
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http://dx.doi.org/10.1016/j.pedn.2022.05.022DOI Listing
June 2022

Lacking of sex-lethal gene lowers the fertility of male reproduction in Spodoptera litura (Lepidoptera).

Pestic Biochem Physiol 2022 Jun 28;184:105087. Epub 2022 Mar 28.

Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Applied Research, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China. Electronic address:

Sex-lethal (Sxl) encodes an RNA-binding protein that acts as the switch of sex determination in Drosophila and influences the genitalia formation and gonadal development. However, its sex-determination roles are not conserved in all insects and its role in the gonadal development of Lepidoptera is not well documented. In this study, three splicing variants of Sxl mRNA were identified in Spodoptera litura and they highly expressed in gonads, particularly in the testis. The mRNA levels of SlSxl exhibited higher expression in the spermatid than the testis sheaths, and gradually increased with the spermiogenesis. Sex-lethal protein (SlSXL) is mainly distributed in the cytoplasm of spermatocytes and the head of spermatid. Knockout of SlSxl resulted in fewer eupyrene sperm bundles and apyrene sperm bundles in the testes of moth and a large number of undeveloped spermatocysts retained in the moth of mutant testis, and leading to the reduction of oviposition and hatch rate in the offsprings after mating with female. These results suggest that SlSxl is a critical player in the spermiogenesis of S. litura.
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http://dx.doi.org/10.1016/j.pestbp.2022.105087DOI Listing
June 2022

Oxidative stress activates transcription of Salmonella pathogenicity island-2 genes in macrophages.

J Biol Chem 2022 Jun 14:102130. Epub 2022 Jun 14.

University of Colorado School of Medicine, Department of Immunology & Microbiology, Aurora, Colorado, USA; Veterans Affairs Eastern Colorado Health Care System, Denver, Colorado, USA. Electronic address:

The type III secretion system encoded in the Salmonella pathogenicity island-2 (SPI-2) gene cluster facilitates intracellular growth of nontyphoidal Salmonella by interfering with the maturation of Salmonella-containing vacuoles along the degradative pathway. SPI-2 gene products also protect Salmonella against the antimicrobial activity of reactive oxygen species (ROS) synthesized by the phagocyte NADPH oxidase (NOX2). However, a potential relationship between inflammatory ROS and the activation of transcription of SPI-2 genes by intracellular Salmonella is unclear. Here we show that ROS engendered in the innate host response stimulate SPI-2 gene transcription. We found the expression of SPI-2 genes in Salmonella-sustaining oxidative stress conditions involves DksA, a protein otherwise known to regulate the stringent response of bacteria to nutritional stress. We also demonstrate the J and zinc-2-oxidoreductase domains of DnaJ as well as the ATPase activity of the DnaK chaperone facilitate loading of DksA onto RNA polymerase complexed with SPI-2 promoters. Furthermore, the DksA-driven transcription of SPI-2 genes in Salmonella experiencing oxidative stress is contingent on upstream OmpR, PhoP, and SsrB signaling events that participate in the removal of nucleoid proteins while simultaneously recruiting RNA polymerase to SPI-2 promoter regions. Taken together, our results suggest the activation of SPI-2 gene transcription in Salmonella subjected to ROS produced by the respiratory burst of macrophages protects this intracellular pathogen against NOX2-mediated killing. We propose that Salmonella have co-opted inflammatory ROS to induce SPI-2-mediated protective responses against NOX2 host defenses.
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http://dx.doi.org/10.1016/j.jbc.2022.102130DOI Listing
June 2022

LncNONMMUG027912 alleviates lipid accumulation through AMPKα/mTOR/SREBP1C axis in nonalcoholic fatty liver.

Biochem Biophys Res Commun 2022 Jun 9;618:8-14. Epub 2022 Jun 9.

Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China; Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Medical University, Wenzhou, Zhejiang, China. Electronic address:

Various metabolic diseases are closely related to lipid metabolism disorders, but the regulatory effect of long noncoding RNAs (lncRNAs) on the function of lipids has been poorly elucidated. Previous our work has found that lncNONMMUG027912 (abbreviated as lnc027912) involved in cholesterol metabolism. Here, we further explored the novel function of lipid metabolism-associated lnc027912. We found that upregulated lnc027912 in AML12 cells treated with oleic acid (OA) and palmitic acid (PA) showed a significant decrease in lipid accumulation, triglyceride (TG) levels, and lipid biosynthesis genes. In terms of regulatory mechanisms, lnc027912 increased the expression of p-AMPKα, inhibited p-mTOR levels, decreased the expression of SREBP1C in nuclei, decreased the promoter activity of SREBP1C, and inhibited the expression of lipid synthesis genes. Most importantly, lnc027912 could reduce lipid accumulation and liver inflammation through AMPKα/mTOR signal axis in nonalcoholic fatty liver disease (NAFLD) mice model. Altogether, our study revealed a novel molecular mechanism of lnc027912 in lipid metabolism through the AMPKα/mTOR/SREBP1C signaling axis and highlights the potential of lnc027912 as a new treatment target for lipid disorder diseases (such as NAFLD).
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http://dx.doi.org/10.1016/j.bbrc.2022.06.029DOI Listing
June 2022

The Relationship Between Famine Exposure During Early Life and Left Ventricular Hypertrophy in Adulthood.

Front Nutr 2022 31;9:898932. Epub 2022 May 31.

Department of Cardiology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Background: Although the evidence was still limited, some studies suggested that childhood malnutrition might affect cardiac function and structure in adulthood. To address the knowledge gap, this study investigated if the Great Chinese Famine exposure during early life had affected left ventricular hypertrophy (LVH).

Methods: This research was a cross-sectional study. It included participants who had cardiac ultrasound assessments and were born in Guangdong, China, from 1 October 1952 to 30 September 1964. They were classified according to their exposure period to famine, namely, no exposure, fetal-, early-, mid-, and late childhood. Multivariate logistic regression and subgroup analysis have been conducted to determine the odds ratio () and confidence intervals (s) between famine exposure and LVH.

Results: This research included 2,543 participants, 1,612 women, their mean age was 59.07 ± 3.65 years, and 704 participants had LVH. LVH prevalence was 122 (23.6%), 87 (25.1%), 133 (27.3%), 184 (29.2%), and 178 (31.7%), in non-, fetal-, early-, mid-, and late-childhood exposed groups, respectively ( = 0.031), while in the non-exposed group, the s for developing carotid plaque as a result of fetal, early-, mid- to late-childhood exposure were 1.08 (95% : 0.76, 1.59, = 0.619), 1.24 (95% : 1.03, 1.79, = 0.031), 1.49 (95% : 1.10, 2.01, = 0.009), and 1.64 (95% : 1.25, 2.18, = 0.001), respectively ( for trend = 0.003). There was no interactive effect between gender, obesity, or hypertension history with how the famine influenced LVH, as the subgroups analyses demonstrated (all for interaction > 0.05).

Conclusion: This research has demonstrated the potential relationship between Great Chinese Famine exposure during childhood and LVH in adults.
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http://dx.doi.org/10.3389/fnut.2022.898932DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9193804PMC
May 2022

Association Between the Children's Dietary Inflammatory Index (C-DII) and Markers of Inflammation and Oxidative Stress Among Children and Adolescents: NHANES 2015-2018.

Front Nutr 2022 27;9:894966. Epub 2022 May 27.

Department of Pediatric Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Objectives: To explore the association of Children's Dietary Inflammatory Index (C-DII) scores with inflammation and markers of inflammatory factors in children and adolescents.

Methods: Data on dietary nutrient intake, markers of inflammation (ferritin, alkaline phosphatase, C-reactive protein (CRP), absolute neutrophil cell count and lymphocyte count) and oxidative stress (serum bilirubin, albumin, and iron) were available for participants aged 6-19 years ( = 1281). Each participant's C-DII score was calculated based on a 24-h diet and recall. Generalized linear models were applied to examine associations between C-DII and markers of inflammation and oxidative stress, while adjusting for covariates. Restricted cubic splines were used to explore the dose-response association of C-DII scores with indicators of inflammatory oxidative stress. Akaike's Information Criterionwas applied to compare the performance of linear and non-linear models.

Results: After adjusting for potential confounders, quantile regression results showed that when comparing C-DII quartile 4 (most pro-inflammatory) and quartile 1 (most anti-inflammatory), lymphocytes, ferritin, CRP were statistically significant differences in serum bilirubin, albumin and serum iron ( < 0.05). The C-DII score showed a non-linear relationship with inflammatory oxidative stress indicators. Overweight/obese children and adolescents who ate a high pro-inflammatory diet were more likely to have higher levels of inflammatory cytokines ( = 0.002).

Conclusions: The dietary inflammatory index in children is associated with markers of chronic inflammation and oxidative stress. A pro-inflammatory diet resulted in increased serum concentrations of these markers, implying that early dietary interventions have implications for reducing chronic inflammation and oxidative stress in children and adolescents.
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http://dx.doi.org/10.3389/fnut.2022.894966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195621PMC
May 2022

Surface-tethered electrochemical biosensor for telomerase detection by integration of homogeneous extension and hybridization reactions.

Talanta 2022 May 28:123597. Epub 2022 May 28.

College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, People's Republic of China. Electronic address:

The general electrochemical biosensors for telomerase detection require the immobilization of primers on the electrode surface for telomeric extension and hybridization reactions. However, immobilization of primers may suffer from the challenges of hindrance effect and configuration freedom, thus reducing the extension and hybridization efficiency. Herein, we developed a sensitive electrochemical biosensor for telomerase detection by integration of homogeneous extension and hybridization reactions and surface-tethered detection. In the presence of telomerase, the biotinylated primer (bio-primer) was efficiently elongated with telomeric repeats of (TTAGGG) at the 3' end in solution. Then, the extension product (bio-DNA) was hybridized with the signal probe DNA modified on the surface of ferrocene (Fc)-capped gold nanoparticle (AuNP). The bio-DNA/DNA/Fc-AuNP hybrids were then tethered by streptavidin-modified electrodes through the specific avidin-biotin interactions, thus producing strong electrochemical signals from the oxidation of Fc tags. The biosensor was successfully used to determine telomerase in HeLa cells and monitor the inhibition efficiency of inhibitor. A wide linear range for the detection of telomerase extracted from HeLa cells was attained. This method has great potential in clinical diagnosis and anti-cancer drug development, and should be beneficial for the fabrication of novel biosensors by integration of homogeneous catalysis and hybridization reactions.
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http://dx.doi.org/10.1016/j.talanta.2022.123597DOI Listing
May 2022

Microplastics separation using stainless steel mini-hydrocyclones fabricated with additive manufacturing.

Sci Total Environ 2022 Sep 14;840:156697. Epub 2022 Jun 14.

Department of Civil and Environmental Engineering, University of California, Irvine, CA 92697-2175, USA; Water-Energy Nexus Centre, University of California, Irvine, CA 92697-2175, USA.

Microplastics have been widely detected in natural and engineered water systems and removing microplastics from various water matrices has become a major challenge. Mini-hydrocyclones (MHCs) have been previously applied to separate mediums of different phases. Given MHCs' capability of separating fine particles from liquid phase, three MHCs were designed and fabricated in stainless steel with 3D printing. Microplastics of densities that were both lower (<1 g·cm) and higher (>1 g·cm) than water's density were used to test the separation efficiency in ultra-purified water. The separation test was performed on single-stage MHC as well as MHCs in series in a closed hydraulic circuit. A range of important operational parameters, including split ratio, feed pressure, feed flow rate, and solid concentration, were evaluated to optimize the separation efficiency. The single-stage MHC experiment revealed that >80 % microplastics >20 μm can be effectively removed at the concentration tested, and the separation efficiency peaked at the split ratio of 35 %. MHCs in series demonstrated their ability to further enhance the separation efficiency of the ones with the same density, as well as separate microplastics of different densities. Mini-hydrocyclones' were also used to separate microplastics in synthetic stormwater, and separation efficiency reached 84 % and 98.1 % for low-density polyethylene (LDPE) and polyamide (PA). The results indicated the MHCs' potential for large-scale application in microplastic separation for industrial and municipal wastewater.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156697DOI Listing
September 2022

Omentin-1 alleviate interleukin-1β(IL-1β)-induced nucleus pulposus cells senescence.

Bioengineered 2022 05;13(5):13849-13859

Department of Orthopaedic Surgery, Jiangyin Hospital Affiliated to Nanjing University of Chinese Medicine, Wuxi, China.

One of the main causes of low back pain (LBP) and degenerative musculoskeletal disorders is intervertebral disc degeneration (IVDD). Inflammation-associated senescence of Human nucleus pulposus cells (HNPCs) plays an essential function in the disease progression of IVDD. Omentin-1 is an adipokine that has been recently reported to have anti-inflammatory potential. In our research, IL-1β was used to simulate the inflammatory environment in the IVDD. We investigated in vitro the effects of Omentin-1 on HNPCs, including the components of senescence, cell cycle and extracellular matrix (ECM) synthesis. The results showed that the addition of Omentin-1 improved IL-1β-induced senescence in HNPCs. G1 phase cell cycle arrest and reduced ECM synthesis in HNPCs. Furthermore, we demonstrated that the effect of Omentin-1 in reducing senescence of HNPCs is dependent on SIRT1. These findings suggest that Omentin-1 plays an important function in protecting HNPCs against senescence and has the potential for IVDD gene target therapy.
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http://dx.doi.org/10.1080/21655979.2022.2084495DOI Listing
May 2022

Coexisting of anti-Ro52 autoantibodies on anti-MDA5 autoantibodies-positive dermatomyositis is highly associated with rapidly progressive interstitial lung disease and mortality risk.

J Rheumatol 2022 Jun 15. Epub 2022 Jun 15.

Department of Rheumatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China; Department of Rheumatology and Immunology, the Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, Jiangsu, China; Department of Rheumatology, Huai'an First People's Hospital, Huai'an, Jiangsu, China; Department of Rheumatology, The First People's Hospital of Changzhou, Changzhou, Jiangsu, China; Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China; Department of Rheumatology, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu, China; Department of Rheumatology, Changzhou No.2 People's Hospital, Changzhou, Jiangsu, China; Department of Rheumatology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China; Department of Rheumatology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China; Department of Rheumatology, Jiangsu Province Hospital of Chinese Medicine, Nanjing, Jiangsu, China; Department of Rheumatology, Nanjing First Hospital, Nanjing, Jiangsu, China; Department of Rheumatology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China; Department of Rheumatology, Xuzhou Central Hospital, Xuzhou, Jiangsu, China; Department of Rheumatology, Yancheng No.1 People's Hospital, Yancheng, Jiangsu, China; Department of Rheumatology, Zhongda Hospital Southeast University, Nanjing, Jiangsu, China; Department of Rheumatology, The Affiliated Suqian First People's Hospital of Nanjing Medical University, Suqian, Jiangsu, China; Department of Rheumatology, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China; Department of Rheumatology, Wuxi No.2 People's Hospital, Wuxi, Jiangsu, China; Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, China, Nanjing, Jiangsu, China. Chengyin Lv and Hanxiao You are joint first authors and contributed equally to this work. Wenfeng Tan and Miaojia Zhang contributed equally to this work. Correspondence to Dr. Wenfeng Tan and Miaojia Zhang, Department of Rheumatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;

Objective: Interstitial lung disease (ILD) is a common extramuscular complication contributing to significant morbidity and mortality in anti-melanoma differentiation associated gene 5 positive dermatomyositis (anti-MDA5+ DM). We conducted this study to investigate the association of anti-Ro52 antibodies with clinical characteristics and prognosis in anti-MDA5+ DM patients.

Methods: We assessed a cohort of 246 patients with anti-MDA5+ DM. To calculate hazard ratios (HRs) and 95% confidence intervals (95% CIs) for RP-ILD and death while controlling for potential confounders, variables selected by univariate COX regression analysis were included in a multivariate COX regression model with the stepwise forward selection method. A 2-tailed value <0.05 was considered to indicate statistical significance.

Results: 246 anti-MDA5+ DM patients were enrolled, 70 cases male, with an average age of 53.10±12.35 years. Anti-Ro52 coexisted in 64.22% (158/246) patients. Anti- Ro52 autoantibodies positive anti-MDA5+ DM patients had a higher rate of RP-ILD (log-rank <0.001) and a higher mortality rate (log-rank=0.010). For anti-MDA5+ DM patients with positive anti-Ro52 antibodies, patients with a short disease course, and high inflammation are at increased risk of RP-ILD and death. The appearance of the active rash is an independent protective factor of death.

Conclusion: Anti-Ro52 antibodies are highly prevalent in anti-MDA5+ DM patients and their coexistence correlates with a higher rate of RP-ILD and mortality. Patients with a short disease course, increased inflammation and without rash are more likely to have a poor prognosis.
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http://dx.doi.org/10.3899/jrheum.220139DOI Listing
June 2022

A switchable Cas12a enabling CRISPR-based direct histone deacetylase activity detection.

Biosens Bioelectron 2022 Jun 9;213:114468. Epub 2022 Jun 9.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha, 410082, PR China. Electronic address:

The efficient and robust signal reporting ability of CRISPR-Cas system exhibits huge value in biosensing, but its applicability for non-nucleic acid analyte detection relies on the coupling of additional recognition modules. To address this limitation, we described a switchable Cas12a and exploited it for CRISPR-based direct analysis of histone deacetylase (HDAC) activity. Starting from the acetylation-mediated inactivation of Cas12a by anti-CRISPR protein AcrVA5, we demonstrated that the acetyl-inactivated Cas12a could be reversibly activated by HDAC-mediated deacetylation based on computational simulations (e.g., deep learning and protein-protein docking analysis) and experimental verifications. By leveraging this switchable Cas12a for both target sensing and signal amplification, we established a sensitive one-pot assay capable of detecting deacetylase sirtuin-1 with sub-nanomolar sensitivity, which is 50 times lower than the standard two-step peptide-based assay. The versability of this assay was validated by the sensitive assessment of cellular HDAC activities in different cell lines with good accuracy, making it a valuable tool for biochemical studies and clinical diagnostics.
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http://dx.doi.org/10.1016/j.bios.2022.114468DOI Listing
June 2022

The Regulatory Role of the Aspergillus flavus Core Retromer Complex in Aflatoxin Metabolism.

J Biol Chem 2022 Jun 10:102120. Epub 2022 Jun 10.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address:

Aflatoxins are a series of highly toxic and carcinogenic secondary metabolites that are synthesized by Aspergillus species. The degradation of aflatoxin enzymes is an important regulatory mechanism which modulates mycotoxin producing. The retromer complex is responsible for the retrograde transport of specific biomolecules and the vacuolar fusion in the intracellular transport. Late endosomal-associated GTPase (Rab7) has been shown to be a downstream effector protein of the retromer complex. A deficiency in the retromer complex or Rab7 results in several cellular trafficking problems in yeast and humans, like protein abnormal accumulation. However, whether retromer dysfunction is involved in aflatoxin synthesis remains unclear. Here, we report that the core retromer complex, which comprises three vacuolar protein sorting-associated proteins (AflVps26-AflVps29-AflVps35), is essential for the development of dormant and resistant fungal forms such as conidia (asexual reproductive spore of fungi) and sclerotia (hardened fungal mycelium), as well as aflatoxin production and pathogenicity, in A. flavus. In particular, we show the AflVps26-AflVps29-AflVps35 complex is negatively correlated with aflatoxin exportation. Structural simulation, site-specific mutagenesis, and co-immunoprecipitation experiments showed that interactions among AflVps26, AflVps29, and AflVps35 played crucial roles in the retromer complex executing its core functions. We further found an intrinsic connection between AflRab7 and the retromer involved in vesicle-vacuole fusion, which in turn affected the accumulation of aflatoxin synthesis-associated enzymes, suggesting that they work together to regulate the production of toxins. Overall, these results provide mechanistic insights that contribute to our understanding of the regulatory role of the core retromer complex in aflatoxin metabolism.
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http://dx.doi.org/10.1016/j.jbc.2022.102120DOI Listing
June 2022

Microplastic pollution at Qilianyu, the largest green sea turtle nesting grounds in the northern South China Sea.

PeerJ 2022 7;10:e13536. Epub 2022 Jun 7.

Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, China.

Microplastics, new persistent pollutants, have recently attracted considerable attention. When present in beach sediments, microplastics may adversely affect the nesting and hatching of sea turtles on beaches. In this study, we investigate microplastic pollution at Qilianyu (northeastern Xisha Islands), the largest known nesting ground for green sea turtles () in China. We found that the average abundance of microplastics in the beach surface sediments was 338.44 ± 315.69 thousand pieces·m or 1,353.78 ± 853.68 pieces·m, with foam and fragments as the main microplastic type identified. The microplastic particles were categorised as small and were predominantly within the 0.05-1 mm size category. Most microplastic particles were white (71.31%). Polystyrene and polyethylene were found to be the most common forms of plastic present. Microplastic pollution was not only observed on the beach surface but also at the bottom of nests approximately 60 cm may be harmful to the incubation of sea turtle eggs. We suggest removing plastic litter, especially small pieces of plastic, on beaches to reduce the threat of microplastic pollution to marine life, including sea turtles. Furthermore, the foam used in aquaculture should be recovered and replaced before it becomes fragmented due to age. In addition, regional cooperation between stakeholders in the South China Sea should be strengthened to collectively promote the reduction and cleanup of marine litter.
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http://dx.doi.org/10.7717/peerj.13536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186329PMC
June 2022

Metformin Ameliorates Senescence of Adipose-Derived Mesenchymal Stem Cells and Attenuates Osteoarthritis Progression via the AMPK-Dependent Autophagy Pathway.

Oxid Med Cell Longev 2022 3;2022:4620254. Epub 2022 Jun 3.

Department of Orthopedics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Osteoarthritis (OA) is one of the most serious age-related diseases worldwide that drastically affects the quality of life of patients. Despite advancements in the treatment of arthritis, especially with adipose-derived mesenchymal stem cells (ADSCs), senescence-induced alterations in ADSCs negatively affect the treatment outcomes. This study was aimed at mechanistically exploring whether metformin could ameliorate the senescence of ADSCs and at exploring the effect of metformin-preconditioned ADSCs in an experimental OA mouse model. In this study, an HO-induced mouse ADSC senescent model was established. Cell proliferation, senescence, and autophagy were investigated in vitro. Moreover, the effects of intra-articular injection of metformin-preconditioned ADSCs were investigated in vivo. Metformin could promote autophagy and activate the AMPK/mTOR pathway in ADSCs. The metformin-enhanced autophagy could improve the survival and reduce the senescence of ADSCs. The protective effects of metformin against senescence were partially blocked by 3-methyladenine and compound C. Injection of metformin-preconditioned ADSCs slowed OA progression and reduced OA pain in mice. The results suggest that metformin activates the AMPK/mTOR-dependent autophagy pathway in ADSCs against HO-induced senescence, while metformin-preconditioned ADSCs can potentially inhibit OA progression.
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http://dx.doi.org/10.1155/2022/4620254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187432PMC
June 2022

Sclerosing thymoma with recurrence of myasthenia gravis: A rare case.

Asian J Surg 2022 Jun 9. Epub 2022 Jun 9.

Department of Pathology, Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.05.054DOI Listing
June 2022

Identification of safe channels for screws in the anterior pelvic ring fixation system.

J Orthop Surg Res 2022 Jun 11;17(1):312. Epub 2022 Jun 11.

The Third Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.

Background: Minimally invasive surgery for pelvic fracture using anterior ring internal fixator system is increasing gradually, and the way to insert the fixation screws in the fixation system is the key technical points of the method. However, there have been few studies on insertion of fixation screws for the anterior pelvic ring internal fixator system.

Objective: To identify safe channels for fixation screws in the anterior pelvic fixator system and provide the anatomical basis for insertion of fixation screws in clinical operation.

Methods: Screw insertion was simulated into a total of 40 pelvic finite element models as well as 16 fresh pelvic specimens, and the channel parameters were measured.

Results: Finite elements (male, female) include: screws in ilium: length 114.4 ± 4.1 and 107.6 ± 8.3 mm, respectively; diameter 11.7 ± 0.5 and 10.0 ± 0.6 mm, distance between screw and anterior inferior iliac spine: 5.5 ± 1.0 and 5.6 ± 1.0 mm, angle of coronal plane 55.8° ± 2.4° and 50.6° ± 3.1°, angle of sagittal plane 26.6° ± 1.0° and 24.5° ± 1.9° and angle of horizontal plane 64.9 ± 3.7 and 58.1 ± 3.1; screws in pubis: length 47.0 ± 2.0 and 39.8 ± 3.9 mm, diameter 7.1 ± 0.4 and 6.1 ± 0.4 mm. Specimens (male, female) include: distance between screw and anterior inferior iliac spine: 5.5 ± 0.5 and 5.6 ± 0.7 mm, angle of coronal plane 55.9° ± 1.3° and 50.7° ± 1.5°, angle of sagittal plane 26.7° ± 0.5° and 24.1° ± 0.9° and angle of horizontal plane 64.8° ± 0.6° and 58.8° ± 0.8°. In the comparison between female and male in each group, differences in distances between screws and anterior inferior iliac spine and median line of symphysis pubis (P > 0.05) were not statistically significant; differences in the remaining parameters were statistically significant (P < 0.05).

Conclusions: If surgeons paid attention to sex differences, select screws of appropriate diameter and length and hold the insertion position and direction, screws in the anterior pelvic ring fixation system could be safely inserted.
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http://dx.doi.org/10.1186/s13018-022-03191-5DOI Listing
June 2022

Enzymological and structural characterization of Arabidopsis thaliana heme oxygenase-1.

FEBS Open Bio 2022 Jun 11. Epub 2022 Jun 11.

School of Life Sciences, Anhui University, Hefei, China.

Arabidopsis thaliana heme oxygenase-1 (AtHO-1), a metabolic enzyme in the heme degradation pathway, serves as a prototype for study of the bilin-related functions in plants. Past biological analyses revealed that AtHO-1 requires ferredoxin-NADP reductase (FNR) and ferredoxin for its enzymatic activity. Here, we characterized the binding and degradation of heme by AtHO-1, and found that ferredoxin is a dispensable component of the reducing system that provides electrons for heme oxidation. Furthermore, we reported the crystal structure of heme-bound AtHO-1, which demonstrates both conserved and previously undescribed features of plant heme oxygenases. Finally, the electron transfer pathway from FNR to AtHO-1 is suggested based on the known structural information.
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http://dx.doi.org/10.1002/2211-5463.13453DOI Listing
June 2022
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