Publications by authors named "Lin Jiang"

1,179 Publications

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Design and Optimization of Thienopyrimidine Derivatives as Potent and Selective PI3Kδ Inhibitors for the Treatment of B-Cell Malignancies.

J Med Chem 2022 May 24. Epub 2022 May 24.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

Phosphoinositide 3-kinase δ (PI3Kδ) plays a critical role in B lymphocyte (B-cell) development and activation and has been a validated target for the treatment of B-cell malignancies. Herein, we report a series of thienopyrimidine derivatives as novel potent and selective PI3Kδ inhibitors based on a scaffold hopping design strategy. Among them, compound exhibited nanomolar PI3Kδ potency and a favorable selectivity profile compared to other class I PI3K isoforms. In cellular assays, compound showed antiproliferative activity against a panel of B-cell lymphoma cell lines in a low micromolar range, caused cell cycle arrest, and induced apoptosis in Pfeiffer and SU-DHL-6 cells. Further, compound inhibited the activation of mouse B-cells. With support from in vivo pharmacokinetic studies, compound demonstrated significant anticancer efficacy in a Pfeiffer xenograft mouse model. Overall, compound is a promising PI3Kδ inhibitor worthy of further preclinical investigation for the treatment of B-cell malignancies.
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http://dx.doi.org/10.1021/acs.jmedchem.2c00530DOI Listing
May 2022

Assessment of the professional dental cleaning knowledge, behavior and medical compliance among dentists, medical doctors and non-medical staffs: a cross sectional study in Chongqing, China.

BMC Oral Health 2022 May 19;22(1):188. Epub 2022 May 19.

College of Stomatology, Chongqing Medical University, Chongqing, People's Republic of China.

Background: The professional dental cleaning (PDC) is an effective supplement that can make up for the lack of self-oral health care. Everyone should develop the habit of regular PDC. This study aimed to investigate the knowledge, behaviors and medical compliance of dentists, medical doctors and non-medical staffs about the PDC, identify the gaps, and provide information to help individuals develop healthy oral hygiene habit.

Materials And Methods: A web-based survey with 21 questions designed based on the characteristics, the PDC knowledge, behaviors, and medical compliance of respondents. A cross-sectional study was conducted in the main districts of Chongqing, China, in the period of September-November 2020. A total of 456 respondents including 153 dentists, 137 medical doctors and 166 non-medical staffs in 3 communities and 4 hospitals were sampled online with multistage sampling and surveyed. The data was analyzed by chi-square test using IBM SPSS Statistics v. 21.0.

Results: The cognitive level and behavior of dentists on the PDC was significantly better than that of medical doctors and non-medical staffs (p = 0.000-0.044). The rates of not knowing "dental plaque" were 32.85% and 61.45%, of the medical doctors and non-medical staffs. Meanwhile, most of them had misunderstanding about effects of the PDC. They thought it would whiten teeth (72.99%, 80.72%), damage gums (16.79%, 19.88%) and teeth (15.33%, 21.69%), and create bigger gaps between teeth (24.82%, 33.13%). In terms of gum health and the PDC behavior, significant differences were observed, 23.53% of dentists experienced irritated gum bleeding in the last 12 months, 42.28% did not have their teeth cleaned professionally in the last 12 months, even 7.84% never had it before. Compared with it, in the same situation, the ratios of medical doctors and non-medical staffs were about 60% experiencing irritated gum bleeding, 69.34% and 77.71% not experiencing the PDC in the last 12 months, up to 33.58% and 45.18% never experienced it before. 41 respondents (8.99%) disagreed with the reasonable and necessary medical behaviors before the PDC.

Conclusion: People's understanding about the PDC was insufficient, and many people, included some dentists, had not developed the habit of regular dental cleaning. Our study provided a new understanding of the PDC among dentists, medical doctors, and non-medical staffs, which may help to improve their awareness and behavior of oral hygiene health, and establish the multidisciplinary collaborations between dentists and medical doctors.
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http://dx.doi.org/10.1186/s12903-022-02226-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118795PMC
May 2022

Metabolomic analysis of honey bee ( L.) response to glyphosate exposure.

Mol Omics 2022 May 18. Epub 2022 May 18.

Division of Natural Sciences, New College of Florida, 5800 Bay Shore Road, Sarasota, FL 34243, USA.

Glyphosate is among the world's most commonly used herbicides in agriculture and weed control. The use of this agrochemical has unintended consequences on non-target organisms, such as honey bees (), the Earth's most prominent insect pollinator. However, detailed understanding of the biological effects in bees in response to sub-lethal glyphosate exposure is still limited. In this study, H NMR-based metabolomics was performed to investigate whether oral exposure to an environmentally realistic concentration (7.12 mg L) of glyphosate affects the regulation of honey bee metabolites in 2, 5, and 10 days. On Day 2 of glyphosate exposure, the honey bees showed significant downregulation of several essential amino acids, including leucine, lysine, valine, and isoleucine. This phenomenon indicates that glyphosate causes an obvious metabolic perturbation when the honey bees are subjected to the initial caging process. The mid-term (Day 5) results showed negligible metabolite-level perturbation, which indicated the low glyphosate impact on active honeybees. However, the long-term (Day 10) data showed evident separation between the control and experimental groups in the principal component analysis (PCA). This separation is the result of the combinatorial changes of essential amino acids such as threonine, histidine, and methionine, while the non-essential amino acids glutamine and proline as well as the carbohydrate sucrose were all downregulated. In summary, our study demonstrates that although no significant behavioral differences were observed in honey bees under sub-lethal doses of glyphosate, metabolomic level perturbation can be observed under short-term exposure when met with other environmental stressors or long-term exposure.
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http://dx.doi.org/10.1039/d2mo00046fDOI Listing
May 2022

Associations of sleep problems with asthma and allergic rhinitis among Chinese preschoolers.

Sci Rep 2022 May 16;12(1):8102. Epub 2022 May 16.

Department of Child Health Care, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 9th Jinsui Road, Tianhe District, Guangzhou, 510623, China.

The aim of this study was to examine the associations of sleep problems with asthma and allergic rhinitis among Chinese preschoolers. This cross-sectional survey was conducted in Guangzhou, China. Children aged 3-6 years were recruited from 32 kindergartens in 7 administrative districts. Asthma, allergic rhinitis and sleep problems were evaluated using a valid questionnaire. Binary logistic regression models were employed to estimate the odds ratios (OR) and 95% confidence intervals (CI) for the associations of asthma and allergic rhinitis with short sleep duration, late bedtime and frequent nocturnal awakening. We included 4876 preschool children in the current analysis. Of these, 182 (3.7%) diagnosed as asthma, and 511 (10.5%) diagnosed as allergic rhinitis. Frequent nocturnal awakening was associated with asthma and allergic rhinitis, with adjusted OR were 1.49 (95% CI 1.05-2.13) and 1.59 (95% CI 1.27-1.99), respectively. Subgroup analysis showed the OR for frequent nocturnal awakening with asthma was higher in girls (1.68; 95% CI 1.02-2.78) than in boys (1.35; 95% CI 0.81-2.24), but the OR for frequent nocturnal awakening with allergic rhinitis were similar in girls (1.73; 95% CI 1.15-2.30) and boys (1.57; 95% CI 1.17-2.12). No significant associations of short sleep duration and late bedtime with asthma or allergic rhinitis were identified. Our data suggested that frequent nocturnal awakening was associated with asthma and allergic rhinitis among preschoolers, and the association of frequent nocturnal awakening with asthma differed by gender. Further studies are warranted to address the causal relationship between nocturnal awakening and asthma and allergic rhinitis.
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http://dx.doi.org/10.1038/s41598-022-12207-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110737PMC
May 2022

Constructing Time-varying Directed EEG network by Multivariate Nonparametric Dynamical Granger Causality.

IEEE Trans Neural Syst Rehabil Eng 2022 May 16;PP. Epub 2022 May 16.

Time-varying directed electroencephalography (EEG) network is the potential tool for studying the dynamical causality among brain areas at a millisecond level; which conduces to understanding how our brain effectively adapts to information processing, giving inspiration to causality- and brain-inspired machine learning. Currently, its construction still mainly relies on the parametric approach such as multivariate adaptive autoregressive (MVAAR), represented by the most widely used adaptive directed transfer function (ADTF). Restricted by the model assumption, the corresponding performance largely depends on the MVAAR modeling which usually encounters difficulty in fitting complex spectral features. In this study, we proposed to construct EEG directed network with multivariate nonparametric dynamical Granger causality (mndGC) method that infers the causality of a network, instead, in a data-driven way directly and therefore avoids the trap in the model-dependent parametric approach. Comparisons between mndGC and ADTF were conducted both with simulation and real data application. Simulation study demonstrated the superiority of mndGC both in noise resistance and capturing the instantaneous directed network changes. When applying to the real motor imagery (MI) data set, distinguishable network characters between left- and right-hand MI during different MI stages were better revealed by mndGC. Our study extends the nonparametric causality exploration and provides practical suggestions for the time-varying directed EEG network analysis.
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http://dx.doi.org/10.1109/TNSRE.2022.3175483DOI Listing
May 2022

Chaotic optical communications at 56 Gbit/s over 100-km fiber transmission based on a chaos generation model driven by long short-term memory networks.

Opt Lett 2022 May;47(10):2382-2385

Chaotic optical communication technology is considered as an effective secure communication technology, which can protect information from a physical layer and is compatible with the existing optical networks. At present, to realize long-distance chaos synchronization is still a very difficult problem, mainly because well-matched hardware cannot always be guaranteed between the transmitter and receiver. In this Letter, we introduce long short-term memory (LSTM) networks to learn a nonlinear dynamics model of an opto-electronic feedback loop, and then apply the trained deep learning model to generate a chaotic waveform for encryption and decryption at the transmitter and receiver. Furthermore, to improve the security, we establish a deep learning model pool which consists of different gain trained models and different delay trained models, and use a digital signal to drive chaos synchronization between the receiver and transmitter. The proposed scheme is experimentally verified in chaotic-encrypted 56-Gbit/s PAM-4 systems, and a decrypted performance below 7%FEC threshold (BER = 3.8×10) can be achieved over a 100-km fiber transmission.
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http://dx.doi.org/10.1364/OL.456258DOI Listing
May 2022

Discrimination of tumor cell type based on cytometric detection of dielectric properties.

Talanta 2022 May 3;246:123524. Epub 2022 May 3.

School of Mechanical Engineering, Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, 211189, China. Electronic address:

In this paper, a microfluidic impedance cytometer (MIC) was employed to analyze the dielectric properties of human white blood cells (WBCs) and four tumor cell lines and realize the label-free identification of cell types. The impedance of cells was detected using an asymmetric serpentine microchannel based MIC under four different frequencies simultaneously. The asymmetric serpentine microchannel achieved the elasto-inertial focusing of cells into a single train, ensuring accurate impedance detection of cells. Various dielectric parameters (cell diameters, impedance amplitude |Z|, impedance phase shift ΦZ, and electric opacities |Z|/|Z|, ΦZ/ΦZ, Re(Z)/Re(Z), and Im(Z)/Im(Z)) were defined and used to analyze the dielectric properties of cells. The obtained dielectric parameters were used to train machine learning classification models for identifying cell types. Using all parameters proposed in this paper (cell diameter, opacity |Z|/|Z|, ΦZ/ΦZ, Re(Z)/Re(Z), and Im(Z)/Im(Z)) to train the classification model, the true positive rate (TPR) for the identification of WBCs, A549, MCF7, H226, and H460 cells were 99.6%, 96.2%, 99.1%, 97.6%, and 97.2%, respectively. Results showed that our MIC provided a promising method for label-free discrimination of circulating tumor cells in multiple primary cancers.
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http://dx.doi.org/10.1016/j.talanta.2022.123524DOI Listing
May 2022

Effect of vapour-solid interfacial adsorption on benzene multiphase partition and its implication to vapour exposure assessment of contaminated soil in arid area.

J Environ Manage 2022 Aug 5;315:115182. Epub 2022 May 5.

National Engineering Research Centre of Urban Environmental Pollution Control, Beijing Key Laboratory for Risk Modeling and Remediation of Contaminated Sites, Beijing Municipal Research Institute of Eco-Environmental Protection, Beijing, 100037, China.

The partitioning of volatile organic compounds (VOCs) in soil multiphase system is a critical process for vapour intrusion, however, the importance of vapour-solid interface adsorption doesn't receive the due attention, which causes the exposure assessment too conservative particularly in arid conditions. This paper proposed a multiphase partitioning equilibrium (MPE) model establishing the quantitative relationship between VOCs and its various partitioning phases in soil, including solid-liquid interface adsorption phase, vapour phase and dissolved phase and vapour-solid interface adsorption phase. Taking benzene as the targeted pollutant, the model was found in good agreement with the experimental data while the errors were within one magnitude basically. The role of vapour-solid interface adsorption under different soil moisture conditions was also investigated by the model. The results reveals that a) soil moisture is the conspicuous controlling factor that affects the benzene partitioning in soil; b) vapour-solid interface adsorption dominates benzene uptake when soil relative saturation (RS) is under 20% among three typical soils; c) as adsorption by soil minerals (vapour-solid interface adsorption) is reduced by increasing amounts of humidity (RS > 20%), uptake by partitioning into the soil organic matter (OM) increasingly becomes a controlling factor; d) the common sense that vapour concentration of benzene is particularly high with low level of RS may not occur since the vapour-solid interface adsorption dominates benzene uptake in arid environment. The MPE model is suitable for prediction of VOCs partitioning and vapour exposure risk assessment of contaminated soil in arid area.
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http://dx.doi.org/10.1016/j.jenvman.2022.115182DOI Listing
August 2022

Exercise more efficiently regulates the maturation of newborn neurons and synaptic plasticity than fluoxetine in a CUS-induced depression mouse model.

Exp Neurol 2022 May 5;354:114103. Epub 2022 May 5.

Laboratory of Stem Cells and Tissue Engineering, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, PR China; Department of Histology and Embryology, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, PR China. Electronic address:

Depression, a common and important cause of morbidity and mortality worldwide, is commonly treated with antidepressants, electric shock and psychotherapy. Recently, increasing evidence has shown that exercise can effectively alleviate depression. To determine the difference in efficacy between exercise and the classic antidepressant fluoxetine in treating depression, we established four groups: the Control, chronic unpredictable stress (CUS/STD), running (CUS/RUN) and fluoxetine (CUS/FLX) groups. The sucrose preference test (SPT), the forced swimming test (FST), the tail suspension test (TST), immunohistochemistry, immunofluorescence and stereological analyses were used to clarify the difference in therapeutic efficacy and mechanism between exercise and fluoxetine in the treatment of depression. In the seventh week, the sucrose preference of the CUS/RUN group was significantly higher than that of the CUS/STD group, while the sucrose preference of the CUS/FLX group did not differ from that of the CUS/STD group until the eighth week. Exercise reduced the immobility time in the FST and TST, while fluoxetine only reduced immobility time in the TST. Hippocampal structure analysis showed that the CUS/STD group exhibited an increase in immature neurons and a decrease in mature neurons. Exercise reduced the number of immature neurons and increased the number of mature neurons, but no increase in the number of mature neurons was observed after fluoxetine treatment. In addition, both running and fluoxetine reversed the decrease in the number of MAP2 dendrites in depressed mice. Exercise increased the number of spinophilin-positive (Sp) dendritic spines in the hippocampal CA1, CA3, and dentate gyrus (DG) regions, whereas fluoxetine only increased the number of SP spines in the DG. In summary, exercise promoted newborn neuron maturation in the DG and regulated neuronal plasticity in three hippocampal subregions, which might explain why running exerts earlier and more comprehensive antidepressant effects than fluoxetine.
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http://dx.doi.org/10.1016/j.expneurol.2022.114103DOI Listing
May 2022

Reduced expression of lncRNA DLEU7-AS1 is a novel favorable prognostic factor in acute myeloid leukemia.

Biosci Rep 2022 May;42(5)

Surgery of Traditional Chinese Medicine, Haian Hospital of Traditional Chinese Medicine, Nantong, Jiangsu, People's Republic of China.

The objective of our study was to measure DLEU7-AS1 expression in de novo acute myeloid leukemia (AML) whilst also analyzing its clinical relevance. We used gene expression data from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), Cancer Cell Line Encyclopedia (CCLE) and Genotype-Tissue Expression project (GTEx) to assess the expression profile of DLEU7-AS1 in pan-cancers, cancer cell lines and normal tissues. Reverse transcription-quantitative PCR was used to measure DLEU7-AS1 expression in bone marrow from 30 normal individuals and 110 patients with de novo AML. DLEU7-AS1 expression was found to be markedly reduced in the AML samples of the TCGA pan-cancer datasets. In our PCR validation, DLEU7-AS1 expression was significantly decreased in the AML samples compared with that in controls (P<0.001). Low DLEU7-AS1 expression (DLEU7-AS1low) correlated positively with lower blood platelet counts (P=0.029). In addition, low DLEU7-AS1 expression was more frequently observed in the intermediate (58%; 44/76) and favorable karyotypes (65%; 15/23) compared with that in the poor karyotype (10%; 1/10; P=0.005). In particular, patients with high expression levels of DLEU7-AS1 (DLEU7-AS1high) showed lower complete remission rates (P=0.002) than patients with DLEU7-AS1low. Survival analysis revealed that patients with DLEU7-AS1low had longer overall survival (OS) than patients with DLEU7-AS1high (P<0.05). Multivariate Cox analysis demonstrated that in patients with non-acute promyelocytic leukemia (non-M3) who were ≤60 years old, DLEU7-AS1 expression was an independent prognostic factor for OS. Furthermore, we found distinct correlations among the expression of DLEU7-AS1, infiltration by immune cells and immune checkpoint genes in AML.
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http://dx.doi.org/10.1042/BSR20212078DOI Listing
May 2022

Polyethylene microplastic exposure and concurrent effect with Aeromonas hydrophila infection on zebrafish.

Environ Sci Pollut Res Int 2022 Apr 25. Epub 2022 Apr 25.

School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, Jiangsu Province, China.

Microplastics are widely distributed in the environment, raising significant concerns owing to their potential negative effects on humans. Zebrafish were used in this study to assess the toxicity of microplastic exposure. Adult zebrafish were exposed to polyethylene (PE) microplastics with smooth clustered sphere shapes and diameters of 75-100 µm for 35 days. Survival rates of the zebrafish were not significantly affected, whereas growth rates were. Analyses on oxidative stress-related enzyme activities showed that glutathione (GSH), glutathione peroxidase (GSH-PX), and glutathione s-transferase (GST) production in the intestines was stimulated when exposed to low concentrations of microplastics (0.1 and 1 mg/L), while superoxide dismutase (SOD), catalase (CAT), GSH, and GSH-PX production was suppressed when exposed to 10 mg/L microplastics. Enzyme activities in the muscles were much less affected. Intestinal injuries and changes in colony structure in the intestines were observed in zebrafish following exposure to microplastics. After 35 days of exposure, concurrent exposure to microplastics and Aeromonas hydrophila did not increase zebrafish mortality compared with those challenged by bacteria alone. This study confirms that intestinal enzyme activities of zebrafish are altered by exposure to PE microplastics but mortality and bacterial infection were not significantly affected under the tested conditions.
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http://dx.doi.org/10.1007/s11356-022-20308-9DOI Listing
April 2022

Powerful and robust inference of complex phenotypes' causal genes with dependent expression quantitative loci by a median-based Mendelian randomization.

Am J Hum Genet 2022 May 22;109(5):838-856. Epub 2022 Apr 22.

Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou 510080, China; Center for Precision Medicine, Sun Yat-sen University, Guangzhou 510080, China. Electronic address:

Isolating the causal genes from numerous genetic association signals in genome-wide association studies (GWASs) of complex phenotypes remains an open and challenging question. In the present study, we proposed a statistical approach, the effective-median-based Mendelian randomization (MR) framework, for inferring the causal genes of complex phenotypes with the GWAS summary statistics (named EMIC). The effective-median method solved the high false-positive issue in the existing MR methods due to either correlation among instrumental variables or noises in approximated linkage disequilibrium (LD). EMIC can further perform a pleiotropy fine-mapping analysis to remove possible false-positive estimates. With the usage of multiple cis-expression quantitative trait loci (eQTLs), EMIC was also more powerful than the alternative methods for the causal gene inference in the simulated datasets. Furthermore, EMIC rediscovered many known causal genes of complex phenotypes (schizophrenia, bipolar disorder, and total cholesterol) and reported many new and promising candidate causal genes. In sum, this study provided an efficient solution to discriminate the candidate causal genes from vast amounts of GWAS signals with eQTLs. EMIC has been implemented in our integrative software platform KGGSEE.
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http://dx.doi.org/10.1016/j.ajhg.2022.04.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118119PMC
May 2022

Effectiveness of Tai Chi Yunshou motor imagery training for hemiplegic upper extremity motor function in poststroke patients: study protocol for a randomized clinical trial.

Trials 2022 Apr 21;23(1):329. Epub 2022 Apr 21.

Shanghai University of Traditional Chinese Medicine Yueyang Hospital of Integrated Traditional Chinese Medicine and Western Medicine, Shanghai, 200437, China.

Background: Evidence concerning the effect of Tai Chi Yunshou motor imagery training (TCY-MIT) on upper extremity motor function (UE-MF) recovery in poststroke patients is lacking, and few studies have examined the neural mechanisms of MIT. The study was designed to assess the effectiveness of TCY-MIT and its possible neural mechanisms.

Methods/design: The study is an assessor-blinded, parallel, superiority, randomized clinical trial. A total of 78 eligible participants will be randomly assigned to 2 groups in a 1:1 ratio. Participants in the control group will receive (conventional rehabilitation therapies) CRTs for 40 min per day, 6 days per week, for 3 weeks. Participants in the intervention group will receive CRTs combined with TCY-MIT (30 min per day, 6 days per week, for 3 weeks). The primary outcome measure is the Fugl-Meyer Assessment of Upper Extremity. Secondary outcome measures are the Box and Block Test, muscle strength test, modified Barthel index, and Pearson correlation coefficients. All outcomes will be assessed at baseline, after completion of the intervention (1, 2, and 3 weeks), and at the end of follow-up (2 months). The outcome assessor will be blinded to the group allocation of the participants.

Discussion: We expect this assessor-blinded, parallel, superiority, randomized clinical trial to explore the effectiveness of TCY-MIT combined with CRTs compared with CRTs alone for UE-MF in poststroke patients.

Trial Registration: Chinese Clinical Trial Registry ID: ChiCTR2100048868 . Registered on 19 July 2021.
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http://dx.doi.org/10.1186/s13063-022-06283-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9022298PMC
April 2022

Does the Geographic Difference of Soil Properties Matter for Setting Up the Soil Screening Levels in Large Countries Like China?

Environ Sci Technol 2022 May 20;56(9):5684-5693. Epub 2022 Apr 20.

National Engineering Research Centre of Urban Environmental Pollution Control, Beijing Key Laboratory for Risk Modeling and Remediation of Contaminated Sites, Beijing Municipal Research Institute of Eco-Environmental Protection, Beijing 100037, China.

China issued the unified national soil screening levels (NSSLs) in 2018 to assist the regulation of contaminated sites, but the applicability of NSSLs was not thoroughly evaluated. Datasets from the National Qinghai-Tibet Plateau Scientific Data Center indicated great variability of soil organic matter (0.8-173 g/kg), soil water content (0.05-0.6), soil porosity (0.4-0.6), and soil bulk density (1.11-1.59 kg/m). We analyzed the effects of soil properties on the derivation of SSLs by using Monte Carlo simulations. The soil factors mainly affected the inhalation exposure pathway of volatile organic compounds (VOCs). They had an effect of more than two orders of magnitude on SSLs for most selected VOCs, particularly with the parameters 0.35 > Henry's law constant > 0.1 and carbon-water distribution coefficient of >100. We compared NSSLs with the recommended SSLs assuming fifth percentile by using Monte Carlo simulations. In general, NSSLs were not sufficient to identify contaminated sites that require additional investigation in the south, central, and northwest regions but were too conservative in screening sites out that required no further action in the east and northeast regions. Our framework and findings may contribute to more scientific and effective soil quality management in other large countries.
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http://dx.doi.org/10.1021/acs.est.1c08771DOI Listing
May 2022

Icariin attenuates renal fibrosis and by inhibiting the Notch2/Hes-1 pathway.

Growth Factors 2022 Apr 15:1-11. Epub 2022 Apr 15.

Department of Nephrology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, China.

Chinese herbs were widely proposed as a novel approach for renal fibrosis. Icariin has been reported to be involved in a variety of diseases. Unilateral ureteral obstruction (UUO) is a popular experimental model of renal injury, which is often used in the study of renal fibrosis. A UUO mouse model was successfully constructed, and tubular injury and renal fibrosis were observed. Icariin treatment attenuated tubular injury and renal fibrosis in UUO mice. In addition, treatment with Icariin reduced the fibronectin, type I collagen and α-SMA levels in UUO mice. Furthermore, in a transforming growth factor (TGF)-β1-induced renal fibrosis cell model, icariin treatment also decreased fibronectin, type I collagen and α-SMA expression. Icariin treatment also reversed the enhanced migration of TGF-β1-induced HK-2 cells. These data indicated that icariin suppressed renal fibrosis in vivo and in vitro. Additionally, icariin treatment suppressed the Notch2/Hes-1 pathway in UUO mice and TGF-β1-treated HK-2 cells. In summary, this study found that icariin reduced renal fibrosis and by inhibiting the Notch2/Hes-1 pathway, which might help to improve therapies for renal fibrosis.
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http://dx.doi.org/10.1080/08977194.2022.2060094DOI Listing
April 2022

Low-carbon economic dispatch considering integrated demand response and multistep carbon trading for multi-energy microgrid.

Sci Rep 2022 Apr 13;12(1):6218. Epub 2022 Apr 13.

Graduate School of Environment and Energy Engineering, Waseda University, Tokyo, 169-8050, Japan.

With the rapid development of distributed energy resources and natural gas power generation, multi-energy microgrid (MEMG) is considered as a critical technology to increase the penetration of renewable energy and achieve the target of carbon emission reduction. Therefore, this paper proposes a low-carbon economic dispatch model for MEMG to minimize the daily operation cost by considering integrated demand response (IDR) and multistep carbon trading. Specifically, IDR operation includes shifting of shiftable electric load, adjusting of flexible thermal load and cooling load, and it is employed to decrease operation cost. Besides, the multistep carbon trading means that different carbon trading prices correspond to different carbon trading volumes, which is applied to stringently restrict carbon emission. The simulation results show that the proposed model can effectively reduce the carbon emission while greatly decrease the operation cost.
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http://dx.doi.org/10.1038/s41598-022-10123-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008055PMC
April 2022

A conditional gene-based association framework integrating isoform-level eQTL data reveals new susceptibility genes for schizophrenia.

Elife 2022 Apr 12;11. Epub 2022 Apr 12.

Program in Bioinformatics, Zhongshan School of Medicine and The Fifth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Linkage disequilibrium and disease-associated variants in the non-coding regions make it difficult to distinguish the truly associated genes from the redundantly associated genes for complex diseases. In this study, we proposed a new conditional gene-based framework called eDESE that leveraged an improved effective chi-squared statistic to control the type I error rates and remove the redundant associations. eDESE initially performed the association analysis by mapping variants to genes according to their physical distance. We further demonstrated that the isoform-level eQTLs could be more powerful than the gene-level eQTLs in the association analysis using a simulation study. Then the eQTL-guided strategies, that is, mapping variants to genes according to their gene/isoform-level variant-gene -eQTLs associations, were also integrated with eDESE. We then applied eDESE to predict the potential susceptibility genes of schizophrenia and found that the potential susceptibility genes were enriched with many neuronal or synaptic signaling-related terms in the Gene Ontology knowledgebase and antipsychotics-gene interaction terms in the drug-gene interaction database (DGIdb). More importantly, seven potential susceptibility genes identified by eDESE were the target genes of multiple antipsychotics in DrugBank. Comparing the potential susceptibility genes identified by eDESE and other benchmark approaches (i.e., MAGMA and S-PrediXcan) implied that strategy based on the isoform-level eQTLs could be an important supplement for the other two strategies (physical distance and gene-level eQTLs). We have implemented eDESE in our integrative platform KGGSEE (http://pmglab.top/kggsee/#/) and hope that eDESE can facilitate the prediction of candidate susceptibility genes and isoforms for complex diseases in a multi-tissue context.
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http://dx.doi.org/10.7554/eLife.70779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005191PMC
April 2022

Preparation and Characterization of New Electrospun Poly(lactic acid) Nanofiber Antioxidative Active Packaging Films Containing MCM-41 Mesoporous Molecular Sieve Loaded with Phloridzin and Their Application in Strawberry Packaging.

Nanomaterials (Basel) 2022 Apr 6;12(7). Epub 2022 Apr 6.

School of Chemistry and Environment, National and Local Joint Engineering Research Center for Green Preparation Technology of Biobased Materials, Yunnan Minzu University, Kunming 650500, China.

Health concerns about food safety have increased in recent years. In order to ensure the safety and increase the shelf-life of food, many methods have been used to slow down the oxidation rate of food fat. In order to solve this problem, a new type of antioxidant-active packaging has emerged. Poly(lactic acid) (PLA) films containing phloridzin adsorbed on to an MCM-41 mesoporous molecular sieve were prepared by electrostatic spinning, using PLA as a film-forming substrate, phloridzin as an antioxidant, and MCM-41 as the adsorption and controlled release carrier. The physical properties of the new films-including microscopic structure, water vapor transmission rate, and fresh-keeping effects, as well as the mechanical, thermal, antioxidant, and antibacterial properties-were studied. When the mass ratio of MCM-41 to phloridzin is 1:2, the nanofiber membrane achieves a 53.61% free-radical scavenging rate and better antibacterial performance (85.22%) due to the high content of phloridzin (30.54%). Additionally, when the mass ratio of the molecular sieve to phloridzin is 1:2 and 3:4 (with the best antibacterial performance of 89.30%), the films significantly delay lipid oxidation in the strawberry packaging, allowing the fresh-keeping time to be extended to up to 21 days before mildew appears. In this study, an MCM-41 mesoporous molecular sieve was used to load phloridzin for the first time. The packaging film with phloridzin, MCM-41, and poly(lactic acid) were used as the raw materials and electrospinning technology was used to prepare the packaging film with antioxidant activity. The packaging film was used for the first time in the packaging of strawberries.
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http://dx.doi.org/10.3390/nano12071229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000760PMC
April 2022

The mouse nicotinamide mononucleotide adenylyltransferase chaperones diverse pathological amyloid client proteins.

J Biol Chem 2022 Apr 7;298(5):101912. Epub 2022 Apr 7.

Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China. Electronic address:

Molecular chaperones safeguard cellular protein homeostasis and obviate proteotoxicity. In the process of aging, as chaperone networks decline, aberrant protein amyloid aggregation accumulates in a mechanism that underpins neurodegeneration, leading to pathologies such as Alzheimer's disease and Parkinson's disease. Thus, it is important to identify and characterize chaperones for preventing such protein aggregation. In this work, we identified that the NAD synthase-nicotinamide mononucleotide adenylyltransferase (NMNAT) 3 from mouse (mN3) exhibits potent chaperone activity to antagonize aggregation of a wide spectrum of pathological amyloid client proteins including α-synuclein, Tau (K19), amyloid β, and islet amyloid polypeptide. By combining NMR spectroscopy, cross-linking mass spectrometry, and computational modeling, we further reveal that mN3 uses different region of its amphiphilic surface near the active site to directly bind different amyloid client proteins. Our work demonstrates a client recognition mechanism of NMNAT via which it chaperones different amyloid client proteins against pathological aggregation and implies a potential protective role for NMNAT in different amyloid-associated diseases.
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http://dx.doi.org/10.1016/j.jbc.2022.101912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108885PMC
April 2022

Correction to: Dihydroartemisinin inhibits TCTPdependent metastasis in gallbladder cancer.

J Exp Clin Cancer Res 2022 Apr 4;41(1):124. Epub 2022 Apr 4.

Department of General Surgery, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Room 517, Building 22, Xinhua Hospital, 1665 Kongjiang Rd., Shanghai, 200092, China.

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http://dx.doi.org/10.1186/s13046-022-02325-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978414PMC
April 2022

Transcriptome Profiling of Developing Ovine Fat Tail Tissue Reveals an Important Role for in Regulation of Adipogenesis.

Front Cell Dev Biol 2022 8;10:839731. Epub 2022 Mar 8.

Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.

Fat-tail sheep exhibit a unique trait whereby substantial adipose tissue accumulates in the tail, a phenotype that is advantageous in many agroecological environments. In this study, we conducted histological assays, transcriptome analysis and functional assays to examine morphogenesis, characterize gene expression, and elucidate mechanisms that regulate fat tail development. We obtained the microstructure of tail before and after fat deposition, and demonstrated that measurable fat deposition occurred by the 80-day embryo (E80) stage, earlier than other tissues. Transcriptome profiling revealed 1,058 differentially expressed genes (DEGs) with six markedly different expression trends. GSEA enrichment and other downstream analyses showed important roles for genes and pathways involving in metabolism and that mitochondrial components were specifically overexpressed in the fat tail tissue of the 70-day embryo (E70). One hundred and eighty-three genes were further identified by leading edge gene analysis, among which, 17 genes have been reported in previous studies, including , , , . Notably, the gene was highly correlated with the expression of other genes and with the highest enrichment score and gene expression change. Knockdown of in isolated adipose derived stem cells (ADSCs) inhibited cell proliferation and migration ability, besides, promoted the process of adipogenesis .
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http://dx.doi.org/10.3389/fcell.2022.839731DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957931PMC
March 2022

Parameter tuning in machine learning based on radiomics biomarkers of lung cancer.

J Xray Sci Technol 2022 ;30(3):477-490

School of Medical Imaging, Tianjin Medical University, Tianjin, China.

Background: Lung cancer is one of the most common cancers, and early diagnosis and intervention can improve cancer cure rate.

Objective: To improve predictive performance of radiomics features for lung cancer by tuning the machine learning model parameters.

Methods: Using a dataset involving 263 cases (125 benign and 138 malignant) acquired from our hospital, each classifier model is trained and tested using 237 and 26 cases, respectively. We initially extract 867 radiomics features of CT images for model development and then test 10 feature selections and 7 models to determine the best method. We further tune the parameter of the final model to reach the best performance. The adjusted final model is then validated using 224 cases acquired from Lung Image Database Consortium (LIDC) dataset (64 benign and 160 malignant) with the same set of selected radiomics features.

Results: During model development, the feature selection via concave minimization method show the best performance of area under ROC curve (AUC = 0.765), followed by l0-norm regularization (AUC = 0.741) and Fisher discrimination criterion (AUC = 0.734). Support vector machine (SVM) and random forest (RF) are the top two machine learning algorithms showing the best performance (AUC = 0.765 and 0.734, respectively), using by the default parameter. After parameter tuning, SVM with linear kernel achieves the best performance (AUC = 0.837), whereas the best tuned RF with the number of trees is 510 and yields a slightly lower performance (AUC = 0.775) in 26 test samples data. During model validation, the SVM and RF models yield AUC = 0.78 and 0.77, respectively.

Conclusion: Appropriate quantitative radiomics features and accurate parameters can improve the model's performance to predict lung cancer.
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http://dx.doi.org/10.3233/XST-211096DOI Listing
April 2022

Synergies between Heat Disturbance and Inoculum Size Promote the Invasion Potential of a Bacterial Pathogen in Soil.

Microorganisms 2022 Mar 16;10(3). Epub 2022 Mar 16.

Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, Key Laboratory of JiangHuai Arable Land Resources Protection and Eco-Restoration, College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China.

Inoculum size contributes to the invasion potential of pathogens in the soil. However, the role of inoculum size in determining the fate of pathogens in disturbed soils remains unclear. Herein, we investigated the survival rates of a bacterial pathogen, , in soils subjected to heat as a simulated disturbance. Our results revealed that heating increased soil resource availability but reduced resource differentiation between and indigenous bacterial communities. In both non-heated and heated soils, invader abundances increased with inoculum size, with a greater magnitude in heated soils. Inoculum size and heat-induced increases in soil-available carbon and nitrogen best predicted invasion success. Altogether, our findings suggested that the invasion by soil pathogens could be predicted by synergies between heat perturbation and inoculum size.
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http://dx.doi.org/10.3390/microorganisms10030630DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950789PMC
March 2022

Using RSM for Optimum of Optimum Production of Peptides from Edible Bird's Nest By-Product and Characterization of Its Antioxidant's Properties.

Foods 2022 Mar 18;11(6). Epub 2022 Mar 18.

School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China.

In this research, the neutrase hydrolysis conditions of edible bird's nest (EBN) by-products were optimized by response surface methodology (RSM). Antioxidant peptides were then isolated from the EBN by-products by ultrafiltration and chromatography taking the DPPH radical scavenging ability as an indicator. The antioxidant activity of the purified peptides was estimated by radical scavenging ability and sodium nitroprusside (SNP)-induced damage model in PC12 cells. When the enzyme concentration was10 kU/g-hydrolysis temperature was 45 °C, and hydrolysis time was 10.30 h, the degree of hydrolysis (DH) of EBN by-product hydrolysate (EBNH) was the highest. The purified peptide exerted strong scavenging ability with EC values of 0.51, 1.31, and 0.65 mg/mL for DDPH, ABTS, and O radicals, respectively. In addition, the purified peptides could significantly reduce the SNP-induced oxidative damage of PC12 cells, and twelve peptides that were rich in leucine (Leu), valine (Val), and lysine (Lys) were identified by LC-MS/MS. These results suggested that EBN by-products have potential as new materials for natural antioxidant peptides.
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http://dx.doi.org/10.3390/foods11060859DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8956092PMC
March 2022

Maize root exudates recruit Bacillus amyloliquefaciens OR2-30 to inhibit Fusarium graminearum infection.

Phytopathology 2022 Mar 17. Epub 2022 Mar 17.

Hefei, China;

Bacillus spp. can exert plant growth-promoting effects and biocontrol effects after effective colonization, and bacterial chemotaxis toward plant root exudates is the initial step to colonize. Under biotic stress, plants are able to alter their root exudates in order to attract or avoid different types of microbes. Hence, Bacillus chemotaxis toward root exudates after pathogen infection is crucial for exerting their beneficial effects. In this study, B. amyloliquefaciens OR2-30 strain, which exhibited greater chemotaxis ability toward maize root exudates after Fusarium graminearum infection, was screened from 156 rhizosphere microorganisms. The infected maize root exudates were further confirmed to improve the swarming and biofilm formation ability of OR2-30 strain. Chemotaxis, swarming and biofilm formation ability were able to influence bacterial colonization. Indeed, the OR2-30 strain displayed more effective colonization ability in maize rhizosphere after F. graminearum inoculation. Moreover, lipopeptides produced by OR2-30 were identified as iturins and responsible for suppressing F. graminearum growth. Further study showed that lipopeptides suppressed the growth of F. graminearum by inhibiting conidia formation and germination, inducing reactive oxygen species (ROS) production and causing cell death in mycelium. Eventually, OR2-30 strain increased maize resistance against F. graminearum. These results suggested that maize root exudates could recruit B. amyloliquefacines OR2-30 after F. graminearum infection, OR2-30 then suppress the F. graminearum by producing lipopeptides, such as iturins, to protect maize.
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http://dx.doi.org/10.1094/PHYTO-01-22-0028-RDOI Listing
March 2022

High Performance of Room-Temperature NbSe Terahertz Photoelectric Detector.

ACS Appl Mater Interfaces 2022 Mar 15;14(12):14331-14341. Epub 2022 Mar 15.

State Key Laboratory of Infrared Physics, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, 500 Yu Tian Road, Shanghai 200083, P. R. China.

Photoelectric detection is developing rapidly from ultraviolet to infrared band. However, terahertz (THz) photodetection approaches is constrained by the bandgap, dark current, and absorption ability. In this work, room-temperature photoelectric detection is extended to the THz range implemented in a planar metal-NbSe-metal structure based on an electromagnetic induced well (EIW) theory, exhibiting an excellent broadband responsivity of 5.2 × 10 V W at 0.027 THz, 7.8 × 10 V W at 0.173 THz, and 9.6 × 10 V W at 0.259 THz. Simultaneously, the NbSe photoelectric detector (PD) with ultrafast response speed (∼610 ns) and ultralow equivalent noise power (4.6 × 10 W Hz) in the THz region is realized, enabling high-resolution imaging. The figure of merit (FOM) characterizing the detection performance of the device is 2 orders of magnitude superior to that of the reported THz PDs based 2D materials. Furthermore, the THz response speed is 2 orders of magnitude faster than that of the visible due to the different response mechanisms of the device. Our results exhibit promising potential to achieve highly sensitive and ultrafast photoelectric detection.
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http://dx.doi.org/10.1021/acsami.2c00175DOI Listing
March 2022

Establishment and metabonomics analysis of nonalcoholic fatty liver disease model in golden hamster.

Z Naturforsch C J Biosci 2022 May 15;77(5-6):197-206. Epub 2022 Mar 15.

Research Center of Natural Resources of Chinese Medicinal Materials and Ethnic Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, China.

The aim is to establish a model of nonalcoholic fatty liver disease (NAFLD) caused by feeding with high-fat, high-fructose, and high-cholesterol diet (HFFCD) in golden hamsters, and to investigate the characteristics of the NAFLD model and metabolite changes of liver tissue. Golden hamsters were fed HFFCD or control diets for six weeks. Body weight, abdominal fat index, and liver index was assessed, serum parameters, hepatic histology, and liver metabolites were examined. The results showed that body weight, abdominal fat, and liver index of hamsters were significantly increased in the model group, the level of serum total cholesterol (TC), triglyceride (TG), and low density lipoprotein-cholesterol (LDL-C) were significantly increased in model group as well, and high density lipoprotein-cholesterol (HDL-C) was significantly decreased. In addition, lipid deposition in liver tissue formed fat vacuoles of different sizes. Metabonomics analysis of the liver showed that the metabolic pathways of sphingolipid, glycerophospholipids, and arginine biosynthesis were disordered in the NAFLD model. The modeling method is simple, short time, and uniform. It can simulate the early fatty liver caused by common dietary factors, and provides an ideal model for the study of the initial pathogenesis and therapeutic drugs for NAFLD.
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http://dx.doi.org/10.1515/znc-2021-0201DOI Listing
May 2022

Panax notoginseng attenuates hypoxia-induced glycolysis in colonic mucosal epithelial cells in DSS-induced colitis.

Ann Transl Med 2022 Feb;10(4):218

Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China.

Background: Colonic mucosal injuries are an important manifestation of ulcerative colitis (UC), which is related to hypoxia-induced glycolysis in colonic mucosal epithelial cells (cmECs). Panax notoginseng (PN) promotes the repair of colonic mucosal injuries by inhibiting hypoxia-induced glycolysis in cmECs; However, the mechanism by which this occurs is not completely clear. Here, we are to investigate the effects of PN on glucose metabolism in cmECs in colitis and the underlying mechanism.

Methods: A model of dextran sulfate sodium-induced colitis rats was used in this research, and the severity of colitis was assessed by pathology, disease activity index (DAI), and weight changes. The content of intracellular pyruvate, intracellular lactate, adenosine triphosphate (ATP), reactive oxygen species (ROS), mitochondrial ROS (mtROS), myeloperoxidase (MPO) activity, superoxide dismutase (SOD) activity, and inflammatory cytokines was detected by assay kits. The expression levels of proteins were detected by western blotting. The expression levels of the ATP4a gene were detected by quantitative polymerase chain reaction (QT-PCR).

Results: The colonic mucosal injuries of the colitis rats were significantly worse than those of the control group. Specifically, the hypoxia-induced glycolysis and potential of hydrogen (pH) in the colonic lumen were increased, and the expression of ATP4a was downregulated in the colitis rats. PN (1.0 g/kg) promoted the repair of colonic mucosal injuries, and reversed the pH in the colonic lumen. Further, PN increased the expression of ATP4a proteins, the content of ATP, and the SOD activity, and decreased the expression of pyruvate dehydrogenase lipoamide kinase isozyme and hypoxia-inducible factor 1-alpha proteins, the content of ROS, and MPO activity in cmECs in colitis. PN also increased the expression of ATP4a, cytochrome P450 family 21 subfamily a member 2, and hydroxy-delta-5-steroid dehydrogenase, 3 beta and steroid delta-isomerase 2 proteins in the mitochondria, and decreased the content of mtROS in cmECs.

Conclusions: PN alleviated the pH in the colonic lumen and hypoxia-induced glycolysis in cmECs by reducing the hypoxia-induced glycolysis caused by the downregulation of ATP4a protein, thereby promoting the repair of colonic mucosal injuries in colitis.
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http://dx.doi.org/10.21037/atm-22-566DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908134PMC
February 2022

Association of aortic distensibility and left ventricular function in patients with stenotic bicuspid aortic valve and preserved ejection fraction: a CMR study.

Int J Cardiovasc Imaging 2022 Mar 12. Epub 2022 Mar 12.

Shanghai Institute of Medical Imaging, Shanghai, 200032, China.

To determine the relationship between aortic distensibility and left ventricular (LV) remodeling, myocardial strain and blood biomarkers in patients with stenotic bicuspid aortic valve (BAV) and preserved ejection fraction (EF) by cardiovascular magnetic resonance (CMR). 43 stenotic BAV patients were prospectively selected for 3.0 T CMR. Patients were divided into LV remodeling group (LV mass/volume ≥ 1.15, n = 21) and non-remodeling group (LV mass/volume < 1.15, n = 22). Clinical characteristics, biochemical data including cardiac troponin T(cTNT), N-terminal pro-B type natriuretic peptide (NT-proBNP) and creatine kinase isoenzyme (CK-MB) were noted. Distensibility of middle ascending aorta (mid-AA) and proximal descending aorta, LV structural and functional parameters, global and regional myocardial strain were measured. Compared to non-remodeling group, LV remodeling group had significantly decreased LV global strain (radial: 26.04 ± 8.70% vs. 32.92 ± 7.81%, P = 0.009; circumferential: - 17.20 ± 3.38% vs. - 19.65 ± 2.34%, P = 0.008; longitudinal: - 9.13 ± 2.34% vs. - 11.63 ± 1.99%, P < 0.001) and decreased mid-AA distensibility (1.22 ± 0.24 10 mm/Hg vs 1.60 ± 0.41 10 mm/Hg, P = 0.001). In addition, mid-AA distensibility was independently associated with LV remodeling (β =  - 0.282, P = 0.003), and it was also significantly correlated with LV global strain (radial: r = 0.392, P = 0.009; circumferential: r =  - 0.348, P = 0.022; longitudinal: r =  - 0.333, P = 0.029), cTNT (r =  - 0.333, P = 0.029) and NT-proBNP (r =  - 0.440, P = 0.003). In this cohort with stenotic BAV and preserved EF, mid-AA distensibility is found significantly associated with LV remolding, which encouraging to better understand mechanism of ventricular vascular coupling.
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http://dx.doi.org/10.1007/s10554-022-02581-4DOI Listing
March 2022

Preliminary Study on the Sequencing of Whole Genomic Methylation and Transcriptome-Related Genes in Thyroid Carcinoma.

Cancers (Basel) 2022 Feb 24;14(5). Epub 2022 Feb 24.

Department of Otolaryngology-Head & Neck Surgery, Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002, China.

Thyroid carcinoma is the most prevalent endocrine cancer globally and the primary cause of cancer-related mortality. Epigenetic modifications are progressively being linked to metastasis. This study aimed to examine whole-genome DNA methylation patterns and the gene expression profiles in thyroid cancer tissue samples using a MethylationEPIC BeadChip (850K), RNA sequencing, and a targeted bisulfite sequencing assay. The results of the Illumina Infinium human methylation kit (850K) analyses identified differentially methylated CpG locations (DMPs) and differentially methylated CpG regions (DMRs) encompassing nearly the entire genome with high resolution and depth. Gene ontology and KEGG pathway analyses revealed that the genes associated with DMRs belonged to various domain-specific ontologies, including cell adhesion, molecule binding, and proliferation. The RNA-Seq study found 1627 differentially expressed genes, 1174 of which that were up-regulated and 453 of which that were down-regulated. The targeted bisulfite sequencing assay revealed that CHST2, DPP4, DUSP6, ITGA2, SLC1A5, TIAM1, TNIK, and ABTB2 methylation levels were dramatically lowered in thyroid cancer patients when compared to the controls, but GALNTL6, HTR7, SPOCD1, and GRM5 methylation levels were significantly raised. Our study revealed that the whole-genome DNA methylation patterns and gene expression profiles in thyroid cancer shed new light on the tumorigenesis of thyroid cancer.
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http://dx.doi.org/10.3390/cancers14051163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909391PMC
February 2022
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