Publications by authors named "Lin Hu"

320 Publications

Identification and validation of a novel locus, Qpm-3BL, for adult plant resistance to powdery mildew in wheat using multilocus GWAS.

BMC Plant Biol 2021 Jul 30;21(1):357. Epub 2021 Jul 30.

Institute of Crop Molecular Breeding/National Engineering Laboratory of Wheat/Key Laboratory of Wheat Biology and Genetic Breeding in Central Huanghuai Area/Ministry of Agriculture/Henan Key Laboratory of Wheat Germplasm Resources Innovation and Improvement, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China.

Background: Powdery mildew (PM), one of the major diseases in wheat, severely damages yield and quality, and the most economical and effective way to address this issue is to breed disease-resistant cultivars. Accordingly, 371 landraces and 266 released cultivars in Henan Province were genotyped by a 660 K microarray and phenotyped for adult plant resistance (APR) to PM from 2017 to 2020, and these datasets were used to conduct multilocus genome-wide association studies (GWASs).

Results: Thirty-six varieties showed stable APR in all the environments, and eleven quantitative trait nucleotides (QTNs) were found by multiple methods across multiple environments and best linear unbiased prediction (BLUP) values to be significantly associated with APR. Among these stable QTNs, four were previously reported, three were newly discovered in this study, and the others need to be further investigated. The major and newly discovered QTN, Qpm-3BL, was located at chr03BL_AX-109,052,670, while another newly discovered QTN, Qpm-1BL, was located between chr01BL_AX-108,771,002 and chr01BL_AX-110,117,322. Five and eight landraces were identified to be resistant based on Qpm-1BL (haplotype TC) and Qpm-3BL (allele T), respectively. To validate Qpm-3BL, a new kompetitive allele-specific PCR (KASP) marker was developed to scan 155 F individuals, and the average resistance score supported the value of Qpm-3BL in marker-assisted breeding. Near Qpm-3BL, PmBMYD was identified by KEGG, gene expression and comparative genomics analyses to be a candidate. Its resistance mechanism may involve gene tandem repeats.

Conclusions: This study reveals a previously unknown gene for PM resistance that is available for marker-assisted breeding.
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http://dx.doi.org/10.1186/s12870-021-03093-4DOI Listing
July 2021

sp. nov., a thermophilic bacterium isolated from compost.

Int J Syst Evol Microbiol 2021 Jul;71(7)

Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.

A Gram-stain-positive, yellow-pigmented, non-motile actinobacterial strain, designated as BIT-GX5, was isolated from a sesame husks compost collected in Beijing, PR China. This bacterium was found to be able to grow in the temperature range from 16 to 50 °C and had an optimal growth temperature at 45 °C. Its taxonomic position was analysed using a polyphasic approach. The 16S rRNA gene sequence (1482 bp) of strain BIT-GX5 was most similar to ATCC BAA-886 (99.45%), LMG 16121 (99.17%) and RS-7-4 (98.75%). The results of phylogenetic analyses, based on the 16S rRNA gene, concatenated sequences of five housekeeping genes (, , , and ) and genome sequences, placed strain BIT-GX5 in a separate lineage among the genus within the family . The major polar lipids of strain BIT-GX5 were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid and aminolipid. The major isoprenoid quinone was MK-9(H), while the cell-wall sugars were galactose, rhamnose, glucose and mannose. The peptidoglycan type was A4α l-Lys-d-Ser-d-Asp. The major fatty acids were anteiso-C and iso-C, which were similar to other members in the genus Results of DNA-DNA hybridization and average nucleotide identity calculations plus physiological and biochemical tests exhibited the genotypic and phenotypic differentiation of strain BIT-GX5 from the other members of the genus . Therefore, strain BIT-GX5 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BIT-GX5 (= CGMCC 1.17687 = KCTC 49391).
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http://dx.doi.org/10.1099/ijsem.0.004905DOI Listing
July 2021

Evaluation of Head Injury Criteria for Injury Prediction Effectiveness: Computational Reconstruction of Real-World Vulnerable Road User Impact Accidents.

Front Bioeng Biotechnol 2021 29;9:677982. Epub 2021 Jun 29.

State Key Laboratory of Advanced Design and Manufacturing for Vehicle Body, Hunan University, Changsha, China.

This study evaluates the effectiveness of various widely used head injury criteria (HICs) in predicting vulnerable road user (VRU) head injuries due to road traffic accidents. Thirty-one real-world car-to-VRU impact accident cases with detailed head injury records were collected and replicated through the computational biomechanics method; head injuries observed in the analyzed accidents were reconstructed by using a finite element (FE)-multibody (MB) coupled pedestrian model [including the Total Human Model for Safety (THUMS) head-neck FE model and the remaining body segments of TNO MB pedestrian model], which was developed and validated in our previous study. Various typical HICs were used to predict head injuries in all accident cases. Pearson's correlation coefficient analysis method was adopted to investigate the correlation between head kinematics-based injury criteria and the actual head injury of VRU; the effectiveness of brain deformation-based injury criteria in predicting typical brain injuries [such as diffuse axonal injury diffuse axonal injury (DAI) and contusion] was assessed by using head injury risk curves reported in the literature. Results showed that for head kinematics-based injury criteria, the most widely used HICs and head impact power (HIP) can accurately and effectively predict head injury, whereas for brain deformation-based injury criteria, the maximum principal strain (MPS) behaves better than cumulative strain damage measure (CSDM and CSDM) in predicting the possibility of DAI. In comparison with the dilatation damage measure (DDM), MPS seems to better predict the risk of brain contusion.
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http://dx.doi.org/10.3389/fbioe.2021.677982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275938PMC
June 2021

Defect-induced ferromagnetism in a S = 1/2 quasi-one-dimensional Heisenberg antiferromagnetic chain compound.

Sci Rep 2021 Jul 14;11(1):14442. Epub 2021 Jul 14.

Anhui Key Laboratory of Condensed Matter Physics at Extreme Conditions, High Magnetic Field Laboratory, Hefei Institutes of Physical Sciences, Chinese Academy of Sciences, Hefei, 230031, Anhui, China.

We present evidences that defects in the spin S = 1/2 Heisenberg antiferromagnetic chain (HAFC) compound can lead to ferromagnetism by studying the magnetic and thermal properties of the newly discovered quasi-one-dimensional (1D) metal-organic framework [CHNH][Cu(HCOO)] (MACuF). Our findings suggest that the long-range ferromagnetic order at 3.7 K can be attributed to Cu ions from the 2D networks constructed by the endpoints of the broken chains. In such a case, the intrinsic magnetism can emerge in this quasi-1D Heisenberg chain system at the background of the short-range antiferromagnetism. This unusual ferromagnetism found in HAFC not only enriches magnetic features in the low-dimensional systems, but helps to understand some of the exotic magnetic phenomena in other real quasi-1D magnetic materials.
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http://dx.doi.org/10.1038/s41598-021-93930-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280101PMC
July 2021

A Unified Catalytic Asymmetric (4+1) and (5+1) Annulation Strategy to Access Chiral Spirooxindole-Fused Oxacycles.

Angew Chem Int Ed Engl 2021 Jun 23. Epub 2021 Jun 23.

Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, Chongqing, 401331, China.

A unified catalytic asymmetric (N+1) (N=4, 5) annulation reaction of oxindoles with bifunctional peroxides has been achieved in the presence of a chiral phase-transfer catalyst (PTC). This general strategy utilizes peroxides as unique bielectrophilic four- or five-atom synthons to participate in the C-C and the subsequent umpolung C-O bond-forming reactions with one-carbon unit nucleophiles, thus providing a distinct method to access the valuable chiral spirooxindole-tetrahydrofurans and -tetrahydropyrans with good yields and high enantioselectivities under mild conditions. DFT calculations were performed to rationalize the origin of high enantioselectivity. The gram-scale syntheses and synthetic utility of the resultant products were also demonstrated.
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http://dx.doi.org/10.1002/anie.202105282DOI Listing
June 2021

Pharmacokinetics, bioavailability and metabolism of neferine in rat by LC-MS/MS and LC-HRMS.

Biomed Chromatogr 2021 Jun 14:e5193. Epub 2021 Jun 14.

Department of Gerontology and Department of Surgery, Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science & Technology, Wuhan, China.

In this study, a simple and sensitive analytical method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated for the determination of neferine in rat plasma. After acetonitrile-mediated protein precipitation, the samples were separated on an Acquity BEH C column (2.1 × 50 mm, 1.7 μm) maintained at 40°C. The mobile phase comprising 0.1% formic acid in water and acetonitrile was delivered at a flow rate of 0.4 ml/min. The mass detection was conducted using multiple reaction monitoring mode with ion transitions at 625.4 > 206.3 and m/z 622.9 > 380.9 for neferine and internal standard, respectively. The assay was demonstrated to be linear over the concentration range of 0.5-1,000 ng/ml, with correlation coefficient >0.999 (r > 0.999). The validated method was further applied to the pharmacokinetic study of neferine in rat plasma. In addition, the metabolism of neferine was investigated using high-resolution mass spectrometry. A total of six metabolites from rat liver microsomes and plasma were detected and their structures were identified according to their fragment ions. The proposed metabolic pathways of neferine were demethylation, dealkylation, dehydrogenation and glucuronidation.
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http://dx.doi.org/10.1002/bmc.5193DOI Listing
June 2021

Myristoylation-mediated phase separation of EZH2 compartmentalizes STAT3 to promote lung cancer growth.

Cancer Lett 2021 Sep 5;516:84-98. Epub 2021 Jun 5.

Institutes of Biological and Medical Sciences, Soochow University Medical School, 199 Ren'ai Road, Suzhou, Jiangsu, 215123, China. Electronic address:

N-myristoylation is a crucial signaling and pathogenic modification process that confers hydrophobicity to cytosolic proteins. Although different large-scale approaches have been applied, a large proportion of myristoylated proteins remain to be identified. EZH2 is overexpressed in lung cancer cells and exerts oncogenic effects via its intrinsic methyltransferase activity. Using a well-established click chemistry approach, we found that EZH2 can be modified by myristoylation at its N-terminal glycine in lung cancer cells. Hydrophobic interaction is one of the main forces driving or stabilizing liquid-liquid phase separation (LLPS), raising the possibility that myristoylation can modulate LLPS by mediating hydrophobic interactions. Indeed, myristoylation facilitates EZH2 to form phase-separated liquid droplets in lung cancer cells and in vitro. Furthermore, we provide evidence that myristoylation-mediated LLPS of EZH2 compartmentalizes its non-canonical substrate, STAT3, and activates STAT3 signaling, ultimately resulting in accelerated lung cancer cell growth. Thus, targeting EZH2 myristoylation may have significant therapeutic efficacy in the treatment of lung cancer. Altogether, these observations not only extend the list of myristoylated proteins, but also indicate that hydrophobic lipidation may serve as a novel incentive to induce or maintain LLPS.
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http://dx.doi.org/10.1016/j.canlet.2021.05.035DOI Listing
September 2021

Magneto-Revealing and Acceleration of Hidden Kirkendall Effect in Galvanic Replacement Reaction.

J Phys Chem Lett 2021 Jun 1;12(22):5294-5300. Epub 2021 Jun 1.

Anhui Key Laboratory of Condensed Matter Physics at Extreme Conditions, High Magnetic Field Laboratory, HFIPS, Anhui, Chinese Academy of Sciences, Hefei, 230031, China.

Rate and product control are crucial for a chemical process and are useful in a wide range of applications. Traditionally, thermodynamic parameters, such as temperature or pressure, have been used to control the chemical reactions. Here, by using the fabrication of a hollow MnFeO nanostructure as a model system, we report an experimental tuning of both chemical reaction rate and product by a high magnetic field. A 12 times magneto-acceleration of the galvanic replacement (GR) reaction was observed. Moreover, it is first demonstrated that a magnetic field can unravel and accelerate the hidden Kirkendall effect (KE) in addition to the pristine GR reaction. With coaction of magneto-tuned KE and GR, not only the rate but also the composition as well as magnetic property of the products could be modulated. These observations suggest that not only is a magnetic field a variable parameter that cannot be ignored, but also it can effectively control both rate and product in a chemical reaction, which provides a new route for chemical process controlling and shape/composition designing in material synthesis.
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http://dx.doi.org/10.1021/acs.jpclett.1c01327DOI Listing
June 2021

The variations in human orphan G protein-coupled receptor QRFPR affect PI3K-AKT-mTOR signaling.

J Clin Lab Anal 2021 Jul 21;35(7):e23822. Epub 2021 May 21.

Wenzhou Key Laboratory of Birth Defects, Wenzhou Central Hospital, Dingli Clinical Medical College of Wenzhou Medical University, Wenzhou, China.

Background: QRFPR is a recently identified member of the G protein-coupled receptor and is an orphan receptor for 26Rfa, which plays important role in the regulation of many physiological functions.

Methods: Here, we employed whole exome sequencing (WES) to examine the patients with intellectual disability (ID) and difficulty in feeding. We performed SIFT and PolyPhen2 predictions for the variants. The structure model was built from scratch by I-TASSER. Here, results derived from a number of cell-based functional assays, including shRNA experiment, intracellular Ca measurement, the expression of PI3 K-AKT-mTOR, and phosphorylation. The functional effect of QRFPR variants on PI3K-AKT-mTOR signaling was evaluated in vitro transfection experiments.

Result: Here, we identified two QRFPR variants at c.202 T>C (p.Y68H) and c.1111C>T (p.R371W) in 2 unrelated individuals. Structural analysis revealed that p.Y68H and p.R371W variants may affect the side chain structure of adjacent amino acids causing reduced binding of QRFPR to 26Rfa. The results show that QRFPR stimulated by 26Rfa leading to the transient rise of intracellular Ca . The QRFPR variations p.Y68H and p.R371 W can reduce the mobilization of intracellular Ca . The phosphorylation levels of the PI3K, Akt, and mTOR were significantly up- or downregulated by QRFPR overexpression or silencing, respectively. The QRFPR variations inhibited PI3K-AKT-mTOR signaling, resulting in downregulation of p-mTOR.

Conclusions: Our findings suggest that QRFPR acts as important role in neurodevelopment, and the effects of QRFPR are likely to be mediated by the Ca -dependent PI3K-AKT-mTOR pathways. Importantly, these findings provide a foundation for future elucidation of GPCR-mediated signaling and the physiological implications.
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http://dx.doi.org/10.1002/jcla.23822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275006PMC
July 2021

Drug-eluting beads bronchial arterial chemoembolization plus intercostals arterial infusion chemotherapy is effective and well-tolerated in treating non-small cell lung cancer patients with refractory malignant pleural effusion.

J Thorac Dis 2021 Apr;13(4):2339-2350

Department of Pulmonary and Critical Care Medicine, The Eighth Medical Center of Chinese PLA General Hospital, College of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, Beijing, China.

Background: The study aimed to explore the efficacy and safety of drug-eluting beads bronchial arterial chemoembolization (DEB-BACE) plus intercostals arterial infusion chemotherapy in non-small cell lung cancer (NSCLC) patients with refractory malignant pleural effusion (MPE).

Methods: 17 NSCLC patients with refractory MPE treated by DEB-BACE plus the intercostals arterial infusion chemotherapy (DEB-BACE group) were recruited. Their treatment response [complete remission (CR), partial remission (PR), overall efficacy, failure] for MPE was assessed at 1 month after therapy; adverse effects were recorded; MPE progression-free survival and overall survival (OS) were calculated. Moreover, 19 NSCLC patients with refractory MPE treated by conventional chemotherapy were reviewed as control (chemotherapy group), then their medical records were collected.

Results: With respect to MPE response, DEB-BACE group exhibited increased CR (82.4% 10.5%, P<0.001) and overall efficacy (100.0% 52.6%, P=0.001), similar PR (17.6% 42.1%, P=0.112) while less failure (0.0% 47.4%, P=0.001) compared to chemotherapy group. Furthermore, OS was prolonged in DEB-BACE group (median: 13.4; 95% CI: 11.0-15.8 months) than chemotherapy group (median: 7.0; 95% CI: 4.4-9.6 months) (P=0.002). Further analyses displayed that in DEB-BACE group, CR was associated with improved ECOG score and longer MPE progression-free survival, and adverse events mainly included fever, chest distress/pain, gastrointestinal side effects, myelosuppression, rash and hemoptysis, which were all mild and tolerable.

Conclusions: DEB-BACE plus intercostals arterial infusion chemotherapy could serve as a salvage treatment option for NSCLC patients with refractory MPE.
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http://dx.doi.org/10.21037/jtd-20-1603DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107566PMC
April 2021

Boosting Hydrazine Oxidation Reaction on CoP/Co Mott-Schottky Electrocatalyst through Engineering Active Sites.

J Phys Chem Lett 2021 May 17;12(20):4849-4856. Epub 2021 May 17.

Hefei National Laboratory for Physical Science at Microscale and Department of Materials Science and Engineering, University of Science and Technology of China, Hefei 230026, People's Republic of China.

The hydrazine oxidation reaction (HzOR), as a substitute for the sluggish oxygen evolution reaction (OER), is identified as a promising powerfrugal strategy for hydrogen production through water splitting. However, the HzOR activity of the present electrocatalysts is unsatisfying because the work potential is much higher than the theoretical value. Herein, we design a typical Mott-Schottky electrocatalyst consisting of CoP/Co nanoparticles for the HzOR, which exhibits remarkable HzOR activity with ultralow potentials of -69 and 177 mV at 10 and 100 mA cm, respectively. It stands out in a range of cobalt-based materials and is even comparable to some precious-metal-based materials composed of Pt or Ru. A shown by with structural characterization and density functional theory (DFT) calculations, the interfaces between CoP/Co nanoparticles not only provide the active sites of HzOR but also promote the multistep dehydrogenation reaction of NH, thus enhancing the HzOR activity.
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http://dx.doi.org/10.1021/acs.jpclett.1c00963DOI Listing
May 2021

Synthesis of 1,3-Aminoalcohols and Spirocyclic Azetidines via Tandem Hydroxymethylation and Aminomethylation Reaction of β-Keto Phosphonates with -Nosyl--(2-bromoethyl)hydroxylamine.

Org Lett 2021 Jun 17;23(11):4152-4157. Epub 2021 May 17.

Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, P. R. China.

An unprecedented tandem α-hydroxymethylation and α-aminomethylation reaction of aromatic cyclic β-keto phosphonates with -nosyl--(2-bromoethyl)hydroxylamine in the presence of DBU base has been developed, affording a range of 1,3-aminoalcohols in good yields. The resultant products could be flexibly transformed into the spirocyclic and bispirocyclic azetidines via one step of Mitsunobu reaction. Mechanistic study revealed that hydroxylamine in situ generated the formaldehyde and nosylamide, which in turn triggered the sequential Horner-Wadsworth-Emmons, Michael, and aldol reactions.
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http://dx.doi.org/10.1021/acs.orglett.1c01091DOI Listing
June 2021

Atomistic Mechanism Underlying the Si(111)-(7×7) Surface Reconstruction Revealed by Artificial Neural-Network Potential.

Phys Rev Lett 2021 Apr;126(17):176101

Department of Materials Science and Engineering, University of Utah, Salt Lake City, Utah 84112, USA.

The 7×7 reconstruction of the Si(111) surface represents arguably the most fascinating surface reconstruction so far observed in nature. Yet, the atomistic mechanism underpinning its formation remains unclear after it was discovered sixty years ago. Experimentally, it is observed post priori so that analysis of its formation mechanism can only be carried out in analogy with archaeology. Theoretically, density-functional theory (DFT) correctly predicts the Si(111)-(7×7) ground state but is impractical to simulate its formation process; while empirical potentials failed to produce it as the ground state. Developing an artificial neural-network potential of DFT quality, we carried out accurate large-scale simulations to unravel the formation of the Si(111)-(7×7) surface. We reveal a possible step-mediated atom-pop rate-limiting process that triggers massive nonconserved atomic rearrangements, most remarkably, a critical process of collective vacancy diffusion that mediates a sequence of selective dimer, corner-hole, stacking-fault, and dimer-line pattern formation, to fulfill the 7×7 reconstruction. Our findings may not only solve the long-standing mystery of this famous surface reconstruction but they also illustrate the power of machine learning in studying complex structures.
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http://dx.doi.org/10.1103/PhysRevLett.126.176101DOI Listing
April 2021

10-Year Incidence of Diabetic Ketoacidosis at Type 1 Diabetes Diagnosis in Children Aged Less Than 16 Years From a Large Regional Center (Hangzhou, China).

Front Endocrinol (Lausanne) 2021 27;12:653519. Epub 2021 Apr 27.

Department of Endocrinology, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, China.

Background: Diabetic ketoacidosis (DKA) is a potentially life-threatening complication of type 1 diabetes (T1D), and a leading cause of death in children aged <15 years with new-onset T1D.

Aims: i) to assess the incidence of DKA in children and adolescents newly diagnosed with T1D over a 10-year period at a large regional center in China; and ii) to examine the clinical symptoms and demographic factors associated with DKA and its severity at diagnosis.

Methods: We carried out a retrospective audit of a regional center, encompassing all youth aged <16 years diagnosed with T1D in 2009-2018 at the Children's Hospital, Zhejiang University School of Medicine (Hangzhou, China). DKA and its severity were classified according to ISPAD 2018 guidelines.

Results: 681 children were diagnosed with T1D, 50.1% having DKA at presentation (36.0% mild, 30.0% moderate, and 33.9% severe DKA). The number of patients diagnosed with T1D progressively rose from approximately 39 cases/year in 2009-2010 to 95 cases/year in 2017-2018 (≈2.5-fold increase), rising primarily among children aged 5-9 years. DKA incidence was unchanged but variable (44.8% to 56.8%). At T1D diagnosis, 89% of patients reported polyuria and 91% polydipsia. Children presenting with DKA were more likely to report vomiting, abdominal pain, and particularly fatigue. DKA was most common among the youngest children, affecting 4 in 5 children aged <2 years (81.4%), in comparison to 53.3%, 42.7%, and 49.3% of patients aged 2-4, 5-9, and ≥10 years, respectively. Children with severe DKA were more likely to report vomiting, fatigue, and abdominal pain, but less likely to report polyuria, polydipsia, and polyphagia than those with mild/moderate DKA. Rates of severe DKA were highest in children aged <2 years (51.1%).

Conclusions: The number of children diagnosed with T1D at our regional center increased over the study period, but DKA rates were unchanged. With 9 of 10 children reporting polyuria and polydipsia prior to T1D diagnosis, increasing awareness of this condition in the community and among primary care physicians could lead to earlier diagnosis, and thus potentially reduce rates of DKA at presentation.
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http://dx.doi.org/10.3389/fendo.2021.653519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112199PMC
April 2021

Outpatient prescribing pattern for acute bronchitis in primary healthcare settings in China.

NPJ Prim Care Respir Med 2021 May 10;31(1):24. Epub 2021 May 10.

Department of Population Medicine, Harvard Medical School and Harvard Pilgrim Health Care Institute, Boston, MA, USA.

Inappropriate prescribing for acute bronchitis in primary healthcare settings (PHSs) is commonly seen worldwide. Here we describe the prescribing patterns and antibiotic use for acute bronchitis in PHSs across China. We conduct a nationwide cross-sectional survey to collect outpatient prescriptions from PHSs in 2017. Patients diagnosed with acute bronchitis without other infections are eligible for this study. Generalized estimating equations are used for analysis. Overall, 10,678 prescriptions for acute bronchitis from 214 institutions are included. The antibiotic prescription rate is 44.5% for total prescriptions, and differs significantly by region and urban/rural status (p < 0.05). Among all single-antibiotic prescriptions, 91.5% are broad-spectrum. Two-thirds of the prescriptions contain medicines for symptom management. The overall guideline compliance rate of acute bronchitis treatment for adults is 31.0%. Prescribing antibiotics, especially broad-spectrum ones, for acute bronchitis is commonly observed in Chinese PHSs. Targeted interventions are urgently needed for Chinese primary clinicians, especially in western rural areas.
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http://dx.doi.org/10.1038/s41533-021-00234-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111021PMC
May 2021

Parameters Indicating Development of Influenza-Associated Acute Necrotizing Encephalopathy: Experiences from a Single Center.

Med Sci Monit 2021 May 2;27:e930688. Epub 2021 May 2.

Department of Pediatric Neurology, Guangzhou Women's and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China (mainland).

BACKGROUND Influenza-associated acute necrotizing encephalopathy (IANE) can be lethal and disabling and have a sudden onset and deteriorate rapidly but lacks early diagnostic indicators. We aimed to examine the early clinical diagnostic indicators in children with IANE. MATERIAL AND METHODS Acute influenza patients were grouped according to their clinical manifestations: flu alone (FA), flu with febrile seizure (FS), influenza-associated encephalopathy (IAE), and IANE. The clinical features, biomarkers, neuroelectrophysiological results, and neuroimaging examination results were compared. RESULTS A total of 31 patients were included (FA (n=4), FS (n=8), IAE (n=14), and IANE (n=5)). The IANE group, whose mean age was 3.7 years, was more likely to show rapid-onset seizure, acute disturbance of consciousness (ADOC), Babinski's sign, and death/sequela. More patients in the IANE group required tracheal intubation mechanical ventilation and received intravenous immunoglobulins (IVIG) and glucocorticoids. The alanine aminotransferase (ALT), aspartate transaminase (AST), and lactate dehydrogenase (LDH) levels in the IANE group were significantly higher than in the FS and IAE groups. The aquaporin-4 (AQP-4) antibody and malondialdehyde (MDA) levels in the serum and cerebrospinal fluid (CSF) were notably higher in IANE patients in the acute stage compared with FS and IAE patients. All patients in the IANE group had positive neuroimaging findings. CONCLUSIONS Early clinical warning factors for IANE include rapid-onset seizures in patients under 4 years of age, ADOC, and pathological signs. Increased AQP-4 antibodies and MDA levels in CSF might contribute to early diagnosis. Early magnetic resonance venography (MRV) and susceptibility-weighted imaging (SWI) sequences, or thrombelastography to identify deep vein thrombosis, might indicate clinical deterioration.
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http://dx.doi.org/10.12659/MSM.930688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101270PMC
May 2021

A novel AIE molecule as a hole transport layer enables efficient and stable perovskite solar cells.

Chem Commun (Camb) 2021 Apr;57(33):4015-4018

College of Chemistry/Institute of Polymers and Energy Chemistry (IPEC), Nanchang University, 999 Xuefu Avenue, Nanchang, 330031, China and Institute of Advanced Scientific Research (iASR), Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, 330022, China.

A low-cost and efficient hole transport layer (HTL) material (TPE-CZ) with the aggregation-induced emission (AIE) effect has been synthesized. Due to the AIE effect, perovskite solar cells with TPE-CZ as the HTL deliver a higher power conversion efficiency (PCE) of 18% with better stability than those with the reference HTL (Spiro-OMeTAD).
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http://dx.doi.org/10.1039/d1cc00484kDOI Listing
April 2021

Clinical application and accuracy analysis of 3D printing guide plate based on polylactic acid in mandible reconstruction with fibula flap.

Ann Transl Med 2021 Mar;9(6):460

Department of Stomatology, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.

Background: Due to the special anatomy morphology and physiological function of the mandible, it has always become a challenge to accurately reconstruct the mandibular defect in maxillofacial surgery. Digital three dimensions (3D) printing surgical guide, as the effective method for individual and accurate surgery, is a hotspot of clinical research at present. In this study, 3D printing PLA surgical guide plate was applied to reconstruct the mandibular defect with fibula flap, its clinical effect and accuracy were evaluated, which aimed to improve the accurate reconstruction of mandibular defects.

Methods: After sterilization, the dimension deformation of the PLA standard specimen were measured. Eighteen patients diagnose with mandibular tumor were collected as observation objects. Then partial mandible resection and simultaneous mandible reconstruction with fibula graft were implemented according to the computer-aided design plan. The clinical effects of 3D printing PLA guide plates application were evaluated by facial contours, occlusal stability and chewing function. Through registering the postoperative computed images reconstruction with preoperative designed shape, the reconstruction accuracy was evaluated by detecting the maximum difference including the distance between lateral convex point of the condyles, the distance between medial convex point of the condyles and the horizontal contained angle between long axis of the condyles.

Results: After high temperature steam sterilization, the curvature of the PLA specimen with 100% filling rate and 4.8 mm thickness were the smallest and their dimension deformation had no statistical significance (P>0.05). The minimally deformed 3D printing PLA guide plate were smoothly placed in the right place during the operation. After surgery, the face was symmetrical, the occlusal relationship was restored well and no deviation of the mandibular movement were found. The spiral computed tomography (SCT) scanning showed that the distance between lateral/medial convex points of the condyle and the horizontal contained angle were 128.34±8.68 mm, 88.69±6.75 mm and 145.87°±12.01°. Compared with preoperative design, the maximum deviation of the actual postoperative registration was 1.67±0.63, and the difference was not statistically significant (P>0.05).

Conclusions: The application of 3D printing PLA guide plate in the segmental section and reconstruction of the mandible can effectively simplify the operation, and better reconstruct the continuity of the mandible. The surgical accuracy can fully meet clinical needs with relatively low prices.
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http://dx.doi.org/10.21037/atm-20-6781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039666PMC
March 2021

Out-of-Plane Alignment of Conjugated Semiconducting Polymers by Horizontal Rotation in a High Magnetic Field.

J Phys Chem Lett 2021 Apr 1;12(14):3476-3484. Epub 2021 Apr 1.

Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, P. R. China.

The effective control of film morphology and molecular packing in the out-of-plane direction of semiconductor polymers plays a critical role in governing charge carrier transport in the direction perpendicular to the substrate. In this study, a highly out-of-plane alignment of the n-type polymer P(NDI2OD-T2) film has been successfully achieved by horizontal rotation in a high magnetic field (HR-HMF). The out-of-plane alignment of the P(NDI2OD-T2) film has showed a change from 72% face-on to 98.2% face-on lamellar texture as well as a 1.6-fold increase of the π-π stacking crystalline correlation length compared with that of as-cast polymer films without HR-HMF-induced alignment. Meanwhile, the film with near-perfect face-on molecular packing exhibited more than 18-fold enhancement of electron mobility compared to the unaligned film. The excellent electrical performance achieved with the HR-HMF process indicates its application potential for fabricating high-performance sandwich-type organic electronic devices, such as solar cells and light-emitting diodes.
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http://dx.doi.org/10.1021/acs.jpclett.1c00385DOI Listing
April 2021

N and O multi-coordinated vanadium single atom with enhanced oxygen reduction activity.

J Colloid Interface Sci 2021 Jul 18;594:466-473. Epub 2021 Mar 18.

Department of Materials Science & Engineering, University of Science and Technology of China, Jinzhai Road NO.96, Hefei, Anhui 230026, PR China; The Anhui High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, PR China. Electronic address:

Recently, atomically dispersed transition-metal single atom in nitrogen-doped carbon matrix as electrocatalysts has aroused general interest. However, there is no report about vanadium single atom for ORR in the literature. According to d-band center theory for transition-metals, the performance of catalysts is regulated by the electronic structure of the catalytic center which determines the intermediate adsorption kinetics. Indeed, the valence of vanadium is variable, its electron structure could be modulated by an appropriate coordination structure. Here, a novel method is developed to prepare the N and O co-coordinated vanadium single atom (V-NO) embedded in the carbon matrix. The catalyst displays a half-wave potential of 865 mV in base solution which surpasses 20% Pt/C, and also shows a high power density of 180 mW/cm in Zn-air batteries. DFT calculations reveal that the N and O coordination configuration could regulate the electron structure and geometry of vanadium to boost the electrocatalytic activity.
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http://dx.doi.org/10.1016/j.jcis.2021.03.074DOI Listing
July 2021

Hierarchical iron molybdate nanostructure array for efficient water oxidation through optimizing electron density.

Chem Commun (Camb) 2021 Apr 11;57(29):3563-3566. Epub 2021 Mar 11.

School of Materials Science and Chemical Engineering, Xi'an Technological University, Xi'an, Shaanxi 710021, China.

The water oxidation reaction plays a major role in many alternative-energy systems because it provides the electrons and protons required for the use of renewable electricity. We report the tuning of the iron molybdate (FeMoO) electron structure via a coupled interface between the catalytic centers and the substrate. Our developed FeMoO catalysts can provide a 50 mA cm current density at 1.506 V vs. RHE with excellent stability in 1.0 M KOH. The improved performance can be ascribed to the synergy of the optimized electronic structures and hierarchical nanostructure.
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http://dx.doi.org/10.1039/d0cc07735fDOI Listing
April 2021

Massive Open Online Courses-based blended versus face-to-face classroom teaching methods for fundamental nursing course.

Medicine (Baltimore) 2021 Mar;100(9):e24829

Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, China.

Abstract: An increasing number of studies focus on the effectiveness of Massive Open Online Courses (MOOC)-based blended learning, whereas none have yet studied using it for teaching fundamental nursing skills at an undergraduate level.To evaluate the effectiveness of MOOC-based blended learning versus face-to-face classroom teaching techniques within the fundamental nursing course at the Faculty of Nursing, University of Xiang Nan, China.This cluster randomized controlled trial enrolled 181 students and assigned them into either an MOOC-based blended or a face-to-face classroom teaching group, both involving the Fundamental Nursing course for undergraduate nursing students. The analyzed outcomes included test scores, critical thinking ability, and feedback received from the students on the Fundamental Nursing course.MOOC-based blended techniques versus face-to-face classroom teaching methods demonstrated higher daily performance (P = .014), operational performance (P = .001), theoretical achievements (P < .001), and final grades (P < .001) in Fundamental Nursing.Moreover, the mean change in the participants' critical thinking ability items between groups were, mostly, statistically significant. The items focusing on the feedback from the students demonstrated significant differences between the groups in terms of their satisfaction with the teaching they received (P < .001) and the overall learning effects (P = .030).This study confirmed that receiving MOOC-based blended learning was superior when compared against face-to-face classroom teaching techniques for learning within the Fundamental Nursing course.
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http://dx.doi.org/10.1097/MD.0000000000024829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939164PMC
March 2021

Mutation analysis and prenatal diagnosis of a family with congenital contractural arachnodactyly.

Mol Genet Genomic Med 2021 Apr 27;9(4):e1638. Epub 2021 Feb 27.

Key Laboratory of Medical Genetics, Wenzhou Central Hospital, Wenzhou, China.

Background: Congenital contractural arachnodactyly (CCA) is a rare autosomal dominant condition caused by mutations in the fibrillin 2 gene (FBN2). The primary clinical symptoms of CCA include multiple flexion contractures, arachnodactyly, dolichostenomelia, scoliosis, abnormal pinnae, muscular hypoplasia, and crumpled ears.

Methods: We used whole-exome sequencing technology to examine an arthrogryposis multiplex congenita and used Sanger sequencing technology to genetically confirm its family.

Results: FBN2 c.3344A>T(p.D1115V) was identified in this family with CCA in a pedigree. Prenatal diagnosis and counseling were carried out simultaneously to avoid the birth of the sick fetus.

Conclusion: The study is on FBN2 variant in CCA, which potentially having implications for genetic counseling and clinical management, our study may provide new insights into the cause and diagnosis of CCA.
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http://dx.doi.org/10.1002/mgg3.1638DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123754PMC
April 2021

Surface doping of non-fullerene photoactive layer by soluble polyoxometalate for printable organic solar cells.

Chem Commun (Camb) 2021 Mar;57(21):2689-2692

Wuhan National Laboratory for Optoelectronics, School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan 430074, China.

The non-fullerene photoactive layer (PTB7-Th:IEICO-4F) film is first immersed into a PMA solution to induce an effective surface p-type doping. An improved hole-collection and a high PCE of 11.37% was obtained, although the non-fullerene OSCs were without a commonly evaporated MoO3. This surface doping technique is an effective and feasible strategy for the printable electronics technology.
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http://dx.doi.org/10.1039/d1cc00032bDOI Listing
March 2021

Sc(OTf)-Catalyzed C-C Bond-Forming Reaction of Cyclic Peroxy Ketals for the Synthesis of Highly Functionalized 1,2-Dioxene Endoperoxides.

Org Lett 2021 Mar 16;23(5):1632-1637. Epub 2021 Feb 16.

Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, P. R. China.

A new and general Sc(OTf)-catalyzed C-C bond-forming reaction of 3-(2-methoxyethoxy)-endoperoxy ketals with silyl ketene acetals, silyl enol ethers, allyltrimethylsilane, and trimethylsilyl cyanide has been developed via the reactive peroxycarbenium ions, affording a wide range of complicated 3,3,6,6-tetrasubstituted 1,2-dioxenes bearing adjacent quaternary carbons and 3-acetyl/allyl/cyano functional groups in good yields at room temperature. Notably, the resultant 1,2-dioxenes are structurally stable, which can be facially transformed into another important 1,2-dioxane endoperoxide under conventional hydrogenation conditions without deconstructing the weak O-O bond.
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http://dx.doi.org/10.1021/acs.orglett.1c00056DOI Listing
March 2021

Spatial transmission network construction of influenza-like illness using dynamic Bayesian network and vector-autoregressive moving average model.

BMC Infect Dis 2021 Feb 10;21(1):164. Epub 2021 Feb 10.

Department of Epidemiology and Health Statistics, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan, China.

Background: Although vaccination is one of the main countermeasures against influenza epidemic, it is highly essential to make informed prevention decisions to guarantee that limited vaccination resources are allocated to the places where they are most needed. Hence, one of the fundamental steps for decision making in influenza prevention is to characterize its spatio-temporal trend, especially on the key problem about how influenza transmits among adjacent places and how much impact the influenza of one place could have on its neighbors. To solve this problem while avoiding too much additional time-consuming work on data collection, this study proposed a new concept of spatio-temporal route as well as its estimation methods to construct the influenza transmission network.

Methods: The influenza-like illness (ILI) data of Sichuan province in 21 cities was collected from 2010 to 2016. A joint pattern based on the dynamic Bayesian network (DBN) model and the vector autoregressive moving average (VARMA) model was utilized to estimate the spatio-temporal routes, which were applied to the two stages of learning process respectively, namely structure learning and parameter learning. In structure learning, the first-order conditional dependencies approximation algorithm was used to generate the DBN, which could visualize the spatio-temporal routes of influenza among adjacent cities and infer which cities have impacts on others in influenza transmission. In parameter learning, the VARMA model was adopted to estimate the strength of these impacts. Finally, all the estimated spatio-temporal routes were put together to form the final influenza transmission network.

Results: The results showed that the period of influenza transmission cycle was longer in Western Sichuan and Chengdu Plain than that in Northeastern Sichuan, and there would be potential spatio-temporal routes of influenza from bordering provinces or municipalities into Sichuan province. Furthermore, this study also pointed out several estimated spatio-temporal routes with relatively high strength of associations, which could serve as clues of hot spot areas detection for influenza surveillance.

Conclusions: This study proposed a new framework for exploring the potentially stable spatio-temporal routes between different places and measuring specific the sizes of transmission effects. It could help making timely and reliable prediction of the spatio-temporal trend of infectious diseases, and further determining the possible key areas of the next epidemic by considering their neighbors' incidence and the transmission relationships.
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http://dx.doi.org/10.1186/s12879-021-05769-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874476PMC
February 2021

Depletion of Survivin suppresses docetaxel-induced apoptosis in HeLa cells by facilitating mitotic slippage.

Sci Rep 2021 Jan 27;11(1):2283. Epub 2021 Jan 27.

The 305 Hospital of the People's Liberation Army, Beijing, 100017, China.

The anticancer effects of taxanes are attributed to the induction of mitotic arrest through activation of the spindle assembly checkpoint. Cell death following extended mitotic arrest is mediated by the intrinsic apoptosis pathway. Accordingly, factors that influence the robustness of mitotic arrest or disrupt the apoptotic machinery confer drug resistance. Survivin is an inhibitor of apoptosis protein. Its overexpression is associated with chemoresistance, and its targeting leads to drug sensitization. However, Survivin also acts specifically in the spindle assembly checkpoint response to taxanes. Hence, the failure of Survivin-depleted cells to arrest in mitosis may lead to taxane resistance. Here we show that Survivin depletion protects HeLa cells against docetaxel-induced apoptosis by facilitating mitotic slippage. However, Survivin depletion does not promote clonogenic survival of tumor cells but increases the level of cellular senescence induced by docetaxel. Moreover, lentiviral overexpression of Survivin does not provide protection against docetaxel or cisplatin treatment, in contrast to the anti-apoptotic Bcl-xL or Bcl-2. Our findings suggest that targeting Survivin may influence the cell response to docetaxel by driving the cells through aberrant mitotic progression, rather than directly sensitizing cells to apoptosis.
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http://dx.doi.org/10.1038/s41598-021-81563-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840972PMC
January 2021

Implementation of STRONGkids for identifying nutritional risk in pediatric intensive care unit: a survey of Chinese practice.

Chin Med J (Engl) 2020 Dec 23;134(7):867-869. Epub 2020 Dec 23.

Department of Critical Care Medicine, West China Hospital, West China School of Nursing, Sichuan University, Chengdu, Sichuan 610041, China.

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http://dx.doi.org/10.1097/CM9.0000000000001330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104208PMC
December 2020

Enhancing carbon dioxide gas-diffusion electrolysis by creating a hydrophobic catalyst microenvironment.

Nat Commun 2021 Jan 8;12(1):136. Epub 2021 Jan 8.

Department of Physics, University of Central Florida, Orlando, FL, USA.

Electroreduction of carbon dioxide (CO) over copper-based catalysts provides an attractive approach for sustainable fuel production. While efforts are focused on developing catalytic materials, it is also critical to understand and control the microenvironment around catalytic sites, which can mediate the transport of reaction species and influence reaction pathways. Here, we show that a hydrophobic microenvironment can significantly enhance CO gas-diffusion electrolysis. For proof-of-concept, we use commercial copper nanoparticles and disperse hydrophobic polytetrafluoroethylene (PTFE) nanoparticles inside the catalyst layer. Consequently, the PTFE-added electrode achieves a greatly improved activity and Faradaic efficiency for CO reduction, with a partial current density >250 mA cm and a single-pass conversion of 14% at moderate potentials, which are around twice that of a regular electrode without added PTFE. The improvement is attributed to a balanced gas/liquid microenvironment that reduces the diffusion layer thickness, accelerates CO mass transport, and increases CO local concentration for the electrolysis.
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http://dx.doi.org/10.1038/s41467-020-20397-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794506PMC
January 2021

Characterization of the complete chloroplast genome of (Liliaceae).

Mitochondrial DNA B Resour 2020 Feb 3;5(1):1046-1047. Epub 2020 Feb 3.

Guangxi Subtropical Crops Research Institute, Nanning, P.R. China.

is a perennial ornamental plant in the family Liliaceae, it is also a valuable medicinal plant. To enrich the genetic resources of , its chloroplast genome was determined by Illumina sequencing data. The chloroplast genome is a typical quadripartite structure with a size of 153,983 bp, of which the LSC region is 83,471 bp, the SSC region is 18,010 bp, and the pair of IR regions is 26,251 bp. The overall GC content is 37%. It contains 131 genes, including 85 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. Phylogenetic analyses showed that is closely related to . However, it can be distinguished from other plants. This study enriches the sequence resources of and provides important data for the development of molecular identification markers.
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http://dx.doi.org/10.1080/23802359.2020.1721362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7748608PMC
February 2020
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