Publications by authors named "Lin Han"

862 Publications

Ultrasensitive Detection of COVID-19 Causative Virus (SARS-CoV-2) Spike Protein Using Laser Induced Graphene Field-Effect Transistor.

Molecules 2021 Nov 17;26(22). Epub 2021 Nov 17.

School of Integrated Circuit, Tsinghua University, Beijing 100084, China.

COVID-19 is a highly contagious human infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and the war with the virus is still underway. Since no specific drugs have been made available yet and there is an imbalance between supply and demand for vaccines, early diagnosis and isolation are essential to control the outbreak. Current nucleic acid testing methods require high sample quality and laboratory conditions, which cannot meet flexible applications. Here, we report a laser-induced graphene field-effect transistor (LIG-FET) for detecting SARS-CoV-2. The FET was manufactured by different reduction degree LIG, with an oyster reef-like porous graphene channel to enrich the binding point between the virus protein and sensing area. After immobilizing specific antibodies in the channel, the FET can detect the SARS-CoV-2 spike protein in 15 min at a concentration of 1 pg/mL in phosphate-buffered saline (PBS) and 1 ng/mL in human serum. In addition, the sensor shows great specificity to the spike protein of SARS-CoV-2. Our sensors can realize fast production for COVID-19 rapid testing, as each LIG-FET can be fabricated by a laser platform in seconds. It is the first time that LIG has realized a virus sensing FET without any sample pretreatment or labeling, which paves the way for low-cost and rapid detection of COVID-19.
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http://dx.doi.org/10.3390/molecules26226947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621829PMC
November 2021

Erratum: Differential Analysis of Hypertension-Associated Intestinal Microbiota.

Int J Med Sci 2021 8;18(16):3748. Epub 2021 Oct 8.

School of Pharmacy, Minzu University of China, Key Laboratory of Ethnomedicine (Minzu University of China), Ministry of education, Beijing 100081, P.R. China.

[This corrects the article DOI: 10.7150/ijms.29322.].
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http://dx.doi.org/10.7150/ijms.65972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579281PMC
October 2021

Phloretin attenuation of hepatic steatosis an improvement of mitochondrial dysfunction by activating AMPK-dependent signaling pathways in C57BL/6J mice and HepG2 cells.

Food Funct 2021 Nov 17. Epub 2021 Nov 17.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, P. R. China.

Phloretin, a dihydrochalcone, widely exists in the fruits of apple trees and crabapple trees () with multiple biological activities. Presently, we studied the function of phloretin on the attenuation of hepatic steatosis and further explored the underlying mechanisms both and . Male C57BL/6J mice were fed a normal diet or high fat diet (HFD) with or without phloretin (100 mg kg) for 12 weeks. HepG2 cells were induced by 200 μM palmitic acid (PA) and co-incubated with phloretin (50 μM) for 24 h. The results showed that phloretin treatment significantly decreased the accumulation of lipids in the liver of the HFD-fed C57BL/6J mice and PA-induced HepG2 cells. Also, phloretin effectively ameliorated hepatic steatosis promoting fatty acid β-oxidation (FAO). This biological activity of phloretin was closely related to its capacity to improve mitochondrial dysfunction, including the promotion of mitochondrial biosynthesis and inhibition of mitochondrial swelling through the AMPK-dependent SIRT1/PGC-1α and SIRT3/CypD signaling pathways, respectively. These results demonstrate that phloretin effectively improves mitochondrial function and ameliorates HFD-induced hepatic steatosis through an AMPK-dependent signaling pathway.
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http://dx.doi.org/10.1039/d1fo02577eDOI Listing
November 2021

Primary Middle Ear Meningioma with Intact Tympanic Membrane: A Case Report and Literature Review.

Ear Nose Throat J 2021 Nov 17:1455613211058100. Epub 2021 Nov 17.

Department of Otorhinolaryngology, Head and Neck Surgery, 71185Peking University People's Hospital, Peking University, Beijing, China.

Primary ectopic meningioma of the middle ear is relatively rare in clinical practice. It is often difficult to distinguish it from chronic otitis media or otitis media with effusion due to its similar and atypical clinical symptoms. We report a case of epithelial tympanic ectopic meningioma with the main complaints of otalgia, aural fullness, and hearing loss. It was accidentally discovered during tympanotomy due to the symptoms of recurring refractory secretory otitis media. This article briefly reviews the relevant literature in recent years, summarizes the characteristics of primary ectopic tympanic meningioma with intact tympanic membrane, and emphasizes the diagnosis and treatment strategy of the middle ear mass.
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http://dx.doi.org/10.1177/01455613211058100DOI Listing
November 2021

Characterization of gut microbiota, metabolism and cytokines in benzene-induced hematopoietic damage.

Ecotoxicol Environ Saf 2021 Nov 12;228:112956. Epub 2021 Nov 12.

Department of Occupational Health and Environmental Health, School of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China. Electronic address:

Benzene exposure leads to hematopoietic dysfunction and is characterized clinically by a decrease in blood cells, but the underlying mechanisms remain elusive. Disturbed gut microbiota may induce host metabolic, immune disorders and the onset of disease. However, the characterization of gut microbiota, metabolism, cytokines and their association with benzene-induced hematopoietic toxicity lacks systematic evidence. Here, the microbiomics, metabolomics and cytokine network were applied to find out the critical characteristics of gut microbiota, metabolism and cytokines in mice involved in the benzene-induced hematopoietic toxicity. We found that the decline in hematopoietic stem cells was earlier than the hematological changes in the 5 mg/kg and 25 mg/kg benzene exposure groups. While 125 mg/kg benzene exposure resulted in a significant decline in whole blood cells. High-throughput sequencing results showed that benzene exposure disrupted homeostasis of gut microbiota, metabolism and cytokine in mice. 6 bacteria, 12 plasma metabolites and 6 cytokines were associated with benzene-induced hematopoietic damage. Notably, IL-5 was significantly increased in benzene exposure group in a dose-dependent manner, and a significant negative correlation was found between IL-5 and hematopoietic damage. We further found that increased Family_XIII_AD3011_group at the genus level and decreased Anaerotruncus_sp at the species level in benzene-exposed group were strongly associated with hematopoietic toxicity and IL-5. Furthermore, the abundance of Family_XIII_AD3011_group and Anaerotruncus_sp were negatively correlated with Adipic acid and 4-Hydroxyproline, respectively. Our findings indicated that altered flora structure of gut microbiota affects the metabolic phenotype which acts as messengers for the gut microbes, affecting host inflammation. This preliminary study provides new insight into the potential mechanisms of benzene-induced hematopoietic toxicity, further exploration by functional studies is required in the future.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112956DOI Listing
November 2021

Surface specifically modified NK-92 cells with CD56 antibody conjugated superparamagnetic FeO nanoparticles for magnetic targeting immunotherapy of solid tumors.

Nanoscale 2021 Nov 25;13(45):19109-19122. Epub 2021 Nov 25.

Key Laboratory for Experimental Teratology of Ministry of Education, Shandong Key Laboratory of Infection and Immunity, and Department of Immunology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, P. R. China.

Although there has been significant progress in the development of tumor immunotherapies, many challenges still exist for the treatment of solid tumors. Natural killer (NK) cells possess broad-spectrum cytotoxicity against tumors, but their limited migration and infiltration abilities restrict their application in solid tumor therapies. Here, we combined a facile and efficient magnetic-targeting strategy with NK cell-based therapy to develop a novel immunotherapy approach for treating solid tumors. Anti-CD56 antibodies were conjugated with FeO nanoparticles, which could specifically bind with NK-92 cells endowing them with a magnetic field driven targeting ability. These NK-FeO biohybrid nanoparticles were able to facilitate directional migration to the tumor site under external magnetic field guidance and efficiently inhibit tumor growth. These functionalized NK cells represent a novel approach for solid tumor therapy and may provide a promising modality for cancer interventions in the future.
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http://dx.doi.org/10.1039/d1nr03329hDOI Listing
November 2021

Effect of extraction method on the chemical profiles and bioactivities of soybean hull polysaccharides.

Food Sci Nutr 2021 Nov 9;9(11):5928-5938. Epub 2021 Sep 9.

College of Food Science and Technology Bohai University Jinzhou China.

The yields, properties, and bioactivities of polysaccharides extracted by three methods from soybean hulls (SSCPs) were evaluated: hot water extraction (H-SSCP), microwave-assisted ammonium oxalate extraction (A-SSCP), and microwave-assisted sodium citrate extraction (S-SSCP). A-SSCP gave the highest yield of polysaccharides (9.3 ± 0.5%) although all three products had similar physicochemical characteristics and FT-IR spectra. A-SSCP and S-SSCP produced polysaccharides with lower molecular weight distributions and higher total reducing power and scavenging ability for ABTS• and DPPH• free radicals. Furthermore, the effect of SSCPs on carbon tetrachloride (CCl)-induced liver injury was investigated in the mice. When compared with H-SSCP and S-SSCP, A-SSCP significantly decreased the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), and reactive oxygen (ROS) to normal ( < .05) and increased the level of glutathione (GSH) to normal ( < .05). A-SSCP was the most effective polysaccharide, yielding an approximately normal hepatic appearance with well-preserved cytoplasm, obvious cell boundaries, with legible nuclei and nucleoli. This study indicates that polysaccharides extracted from soybean hulls via microwave-assisted ammonium oxalate extraction have the potential to be developed as a new functional food contributing to the alleviation of liver damage.
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http://dx.doi.org/10.1002/fsn3.2483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565245PMC
November 2021

The Prevalence and Factors Associated With Anxiety-Like and Depression-Like Behaviors in Women With Polycystic Ovary Syndrome.

Front Psychiatry 2021 20;12:709674. Epub 2021 Oct 20.

Department of Gynecology of Traditional Chinese Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Increasing evidence shows that polycystic ovary syndrome (PCOS) patients are particularly vulnerable to anxiety/depression-like behaviors. This study sought to determine the prevalence of anxiety/depression-like behaviors among women with PCOS and to identify factors associated with these behaviors. This study was a secondary analysis of three studies performed on Chinese women who were aged 18 to 40 and diagnosed with PCOS according to the modified Rotterdam criteria. We obtained 802 useable responses for the self-rating anxiety scale and 798 responses for the self-rating depression scale. The prevalence of anxiety-like and depression-like behaviors among women with PCOS was 26.1% (209/802) and 52.0% (415/798), respectively. Anxiety-like behaviors were associated with age, body image-related factors (including body mass index and waist-to-hip ratio), and hyperandrogenism-related factors (including free androgen index and hirsutism). Depression-like behaviors were associated with age, body image-related factors, hyperandrogenism-related factors, and metabolic factors (including fasting insulin, fasting plasma glucose, and homeostatic model assessment of insulin resistance). Body image-related factors and hyperandrogenism-related factors were related to both anxiety-like behaviors and depression-like behaviors in both infertile and fertile PCOS patients.
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http://dx.doi.org/10.3389/fpsyt.2021.709674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563587PMC
October 2021

Living Bacterial Hydrogels for Accelerated Infected Wound Healing.

Adv Sci (Weinh) 2021 Oct 31:e2102545. Epub 2021 Oct 31.

Central Laboratory, Shanghai Tenth People's Hospital, Tongji University, 301# Yanchang Middle Road, Shanghai, 200072, China.

Damaged skin cannot prevent harmful bacteria from invading tissues, causing infected wounds and even serious tissue damage. Traditional treatments can not only kill pathogenic bacteria, but also suppress the growth of beneficial bacteria, thus destroying the balance of the damaged skin microbial ecosystem. Here, a living bacterial hydrogel scaffold is reported that accelerates infected wound healing through beneficial bacteria secreting antibacterial substances. Lactobacillus reuteri, a common probiotic, is encapsulated in hydrogel microspheres by emulsion polymerization and further immobilized in a hydrogel network by covalent cross-linking of methacrylate-modified hyaluronic acid. Owing to light-initiated crosslinking, the hydrogel dressing can be generated in situ at the wound site. This hydrogel scaffold not only protects bacteria from immune system attack, but also prevents bacteria from escaping into the local environment, thus avoiding potential threats. Both in vitro and in vivo experiments show that it has excellent ability against harmful bacteria and anti-inflammatory capabilities, promoting infected wound closure and new tissue regeneration. This work may open up new avenues for the application of living bacteria in the clinical management of infected wounds.
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http://dx.doi.org/10.1002/advs.202102545DOI Listing
October 2021

Limited Role of Endoscopic Biliary Drainage Before Steroid Treatment for Autoimmune Pancreatitis With Significant Obstructive Jaundice.

Pancreas 2021 Sep;50(8):e65-e66

Digestive Endoscopy Center, Department of Gastroenterology, Changhai Hospital, Naval Medical University, Shanghai, China or

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http://dx.doi.org/10.1097/MPA.0000000000001883DOI Listing
September 2021

Tongluo Yishen Decoction Ameliorates Renal Fibrosis Regulating Mitochondrial Dysfunction Induced by Oxidative Stress in Unilateral Ureteral Obstruction Rats.

Front Pharmacol 2021 12;12:762756. Epub 2021 Oct 12.

Institute of Basic Theory for Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, China.

Tongluo Yishen (TLYS) decoction is an herb that is extensively applied for the treatment of chronic kidney disease (CKD) in traditional Chinese medicine. In this study, 37 different dominant chemical constituents of TLYS were identified. Rats with unilateral ureteral obstruction (UUO) were used as animal models, and TLYS decoction was administered orally for 14 days. TLYS decoction reduced the levels of renal function indicators, serum creatinine levels and blood urea nitrogen levels and alleviated renal pathological changes. Gene Ontology (GO) and KEGG pathway analyses of RNA sequencing data showed that TLYS decoction had significant effects on biological processes, cellular components and molecular functions in UUO rats and that the phagosome (a membrane source in the early stages of autophagy), lysosome (an important component of autolysosome), and oxidation pathways (which contribute to mitochondrial function) might be related to the antifibrotic effects of TLYS decoction. Moreover, we found significant mitochondrial function impairment, including a decreased mitochondrial membrane potential (MMP) and an imbalance in mitochondrial dynamics, excessive oxidative stress, and activation of Pink1/Parkin-mediated mitophagy in UUO rats. Treatment with TLYS decoction significantly increased the MMP, normalized mitochondrial dynamics and ameliorated renal injury. Moreover, TLYS alleviated the mitophagy clearance deficiency. In conclusion, our study showed that TLYS decoction can ameliorate mitochondrial dynamics by reducing oxidative stress and regulating mitophagy, thereby relieving renal injury, protecting renal function, and reducing renal fibrosis. This study provides support for the application of and further research on TLYS decoction.
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http://dx.doi.org/10.3389/fphar.2021.762756DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8545824PMC
October 2021

Low-cost portable bioluminescence detector based on silicon photomultiplier for on-site colony detection.

Anal Chim Acta 2021 Nov 18;1185:339080. Epub 2021 Sep 18.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, PR China. Electronic address:

A low-cost, portable bioluminescence detector based on a silicon photomultiplier (SiPM) was developed for on-site colony detection, the main components of which are a low-noise photoelectric signal detection and processing circuit, power management module, and high-performance embedded microcontroller subsystem with peripheral circuits. Balanced chopper modulation and lock-in amplification techniques were adopted to improve the signal-to-noise ratio, and a zero-adjustment technique was used to eliminate the dark current of the SiPM to expand the dynamic range. Using this bioluminescence detector, adenosine triphosphate could be determined in the range of 3.6 × 10 to 3.6 × 10 mol/L, and bacterial colonies could be determined in the range of 1.0 × 10 to 1.0 × 10 CFU/mL, with a limit of quantitation of 1.0 × 10 CFU/mL. Satisfactory recoveries and precision were obtained. Actual samples were accurately tested and the data were verified by comparison with those from the national standard method. The manufacturing cost of the bioluminescence detector was only $30, which is only approximately 1% of the price of current commercial instruments. This study provides a tool for rapid on-site detection of bacterial colonies, as well as a new concept for the development of low-cost portable detection equipment.
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http://dx.doi.org/10.1016/j.aca.2021.339080DOI Listing
November 2021

Asymmetric Hydrogenation of Racemic α-Aryl-β-ethoxycarbonyl Cyclopentanones via Dynamic Kinetic Resolution and Its Application to the Synthesis of (+)-Burmaniol A.

Org Lett 2021 Nov 28;23(22):8883-8887. Epub 2021 Oct 28.

State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.

An efficient asymmetric hydrogenation of racemic α-aryl-β-ethoxycarbonyl cyclopentanones via dynamic kinetic resolution is reported. Via catalysis by a chiral iridium Ir-SpiroPAP catalyst, a range of racemic α-aryl-β-ethoxycarbonyl cyclopentanones were hydrogenated to the corresponding ester-functionalized chiral 2-arylcyclopentanols with three contiguous stereocenters in high yields with excellent enantio- and diastereoselectivities. This method was successfully applied in the enantioselective synthesis of cyclopentane-based γ-amino ester/alcohol derivatives and phenylpropanoid (+)-burmaniol A.
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http://dx.doi.org/10.1021/acs.orglett.1c03384DOI Listing
November 2021

A CRISPR/Cas12a-Mediated Dual-Mode Electrochemical Biosensor for Polymerase Chain Reaction-Free Detection of Genetically Modified Soybean.

Anal Chem 2021 11 26;93(44):14885-14891. Epub 2021 Oct 26.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, P.R. China.

A clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a-mediated dual-mode electrochemical biosensor without polymerase chain reaction (PCR) amplification was designed for sensitive and reliable detection of genetically modified soybean SHZD32-1. A functionalized composite bionanomaterial [email protected]/DNA-Fc&Ru was synthesized as the signal unit, while a characteristic gene fragment of SHZD32-1 was chosen as the target DNA (tDNA). When Cas12a, crRNA, and tDNA were present simultaneously, a ternary complex Cas12a-crRNA-tDNA was formed, and the nonspecific cleavage ability of the CRISPR/Cas12a system toward single-stranded DNA was activated. Thus, the single-stranded DNA-Fc in the signal unit was cleaved, resulting in the decrease in the fast scan voltammetric (FSV) signal from ferrocene (Fc) and the increase in the electrochemiluminescence (ECL) signal from ruthenium complex (Ru) inhibited by Fc. The linear range was 1-10 fmol/L for ECL and 10-10 fmol/L for FSV, and the limit of detection (LOD) was 0.3 fmol/L for ECL and 3 fmol/L for FSV. Accuracy, precision, stability, selectivity, and reliability were all satisfied. In addition, PCR-free detection could be completed in an hour at room temperature without requiring complicated operation and sample processing, showing great potential in the field detection of genetically modified crops.
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http://dx.doi.org/10.1021/acs.analchem.1c04022DOI Listing
November 2021

Acrylate protects a marine bacterium from grazing by a ciliate predator.

Nat Microbiol 2021 Nov 25;6(11):1351-1356. Epub 2021 Oct 25.

College of Marine Life Sciences, and Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, China.

Cleavage of dimethylsulfoniopropionate (DMSP) can deter herbivores in DMSP-producing eukaryotic algae; however, it is unclear whether a parallel defence mechanism operates in marine bacteria. Here we demonstrate that the marine bacterium Puniceibacterium antarcticum SM1211, which does not use DMSP as a carbon source, has a membrane-associated DMSP lyase, DddL. At high concentrations of DMSP, DddL causes an accumulation of acrylate around cells through the degradation of DMSP, which protects against predation by the marine ciliate Uronema marinum. The presence of acrylate can alter the grazing preference of U. marinum to other bacteria in the community, thereby influencing community structure.
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http://dx.doi.org/10.1038/s41564-021-00981-1DOI Listing
November 2021

The Effect of Zbxz23ir-21 NANO(nanomaterials) Delivery Vector on Apoptosis and PTEN(phosphatase and tensin homolog deleted on chromosome ten)/PI3K(Intracellular phosphatidylinositol kinase)/AKT(related to the A and C kinase) in Children with CHOLESTEATOMA in Middle Ear.

Bioengineered 2021 12;12(1):8809-8821

Department of Otolaryngology, Peking University People's Hospital, Beijing, China.

Cholesteatoma of the middle ear is a kind of cystic disease with clear boundary formed by the abnormal growth of keratosquamous epithelium in temporal bone. Cholesteatoma otitis caused by it is a common disease in otorhinolaryngology. The EPR effect promotes the selective distribution of macromolecular substances in tumor tissues, which can increase drug efficacy. The purpose of this paper is to prepare and deliver the mir34a small molecule regulator, rubine, by nanotechnology, and to deliver it to the cells successfully. It can passively target tumor tissue through EPR effect, and play its regulatory role on miR-34a, thus inhibiting the growth of cholesteatoma cells. The effects of nano delivery on apoptosis and PIEN/P13K/AKt of children with middle ear choledochoma were tested in this paper. The experimental results were conducted on cholesteatoma cells as cell lines and balb/c nude mice as experimental objects. The expression of PTEN/PI3K/AKT in experimental group and control group was detected by immunohistochemistry. Apoptosis was discussed by cell activity detection. The physical and chemical properties, encapsulation efficiency, drug release ability in vitro and antitumor activity of nanoparticles in vitro and in vivo were studied. The results of cell level experiments in vitro showed that free RUBINE caused about 15% apoptosis, which was not different from RC NPs. The results showed that the nanoparticles could improve the expression of miR-34 in the cells, and then regulate the expression of Bcl-2, Cdk6 and CyclinD1, and play the inhibitory effect of miR-34a on the proliferation and migration of tumor cells.
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http://dx.doi.org/10.1080/21655979.2021.1984718DOI Listing
December 2021

Case Report: Novel Compound-Heterozygous Variants of Gene that Cause Trichohepatoenteric Syndrome 2.

Front Genet 2021 6;12:756451. Epub 2021 Oct 6.

Division of Gastroenterology, Shenzhen Children's Hospital, Shenzhen, China.

Trichohepatoenteric syndrome (THES) is a rare disease that mainly causes intractable diarrhea. It is classified into THES1 and THES2, which are associated with the tetratricopeptide repeat domain 37 (TTC37) gene and Ski2-like RNA helicase (SKIV2L) gene, respectively. THES is not very prevalent in China or worldwide, but new cases have increasingly been reported. Here, we report the clinical and genetic information of a 1.5-month-old girl who was admitted to our hospital due to diarrhea and failure to thrive. Whole-exome sequencing (WES) revealed novel compound-heterozygous variants of the SKIV2L gene, c.3602_3609delAGCGCCTG (p.Q1201Rfs*2), and c.1990A > G (p.T664A) as the causative factors, which were confirmed via Sanger sequencing. Upon continuous feeding with an amino-acid formula through a gastric tube and parenteral nutrition, the patient resumed thriving and her stool frequency decreased. We report a girl carrying novel variants of the gene that cause THES2, thereby providing valuable information on the diagnosis of THES2 and expanding the spectrum of disease-causing mutations.
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http://dx.doi.org/10.3389/fgene.2021.756451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8527088PMC
October 2021

Levels of Serum sST2, MMP-3, and Gal-3 in Patients with Essential Hypertension and Their Correlation with Left Ventricular Hypertrophy.

Evid Based Complement Alternat Med 2021 12;2021:7262776. Epub 2021 Oct 12.

Department of Cardiology, Beijing Traditional Chinese Medicine Hospital Affiliated to Capital Medical University, Beijing 100010, China.

Essential hypertension (EH) is a clinically frequent cardiovascular disease, with insidious onset, causing increased pressure load and neuroregulation disorders in patients. Long-term EH can cause left ventricular hypertrophy (LVH), which can lead to arrhythmia and even death. The soluble suppression of tumorigenicity 2 (sST2), matrix metalloproteinase-3 (MMP-3), and galectin-3 (Gal-3) in serum plays an important role in the occurrence, development, and prognosis of cardiovascular diseases. In our study, we divided EH patients into 3 levels and groups with or without LVH, according to their condition. The levels of sST2, MMP-3, and Gal-3 in the serum were measured in different groups of patients. Our results showed that the levels of sST2, MMP-3, and Gal-3 in the serum increased progressively with the level in different EH groups. The levels of sST2, MMP-3, and Gal-3 in the serum of the LVH group were higher than those of the NLVH group, and it is positively correlated with LVH-related indexes. The risk of developing and progressing to LVH in patients with EH can be determined by the method of measuring three indicators.
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http://dx.doi.org/10.1155/2021/7262776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526212PMC
October 2021

Double-Walled [email protected] Multicomponent Senary Metal-Organic Polyhedral Framework and Its Isoreticular Evolution.

J Am Chem Soc 2021 Nov 19;143(43):17942-17946. Epub 2021 Oct 19.

Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, United States.

Metal-organic polyhedral frameworks are attractive in gas storage and separation due to large voids with windows that can serve as traps for guest molecules. Introducing multivariant/multicomponent functionalities in them are ways of improving performances for certain targets. The high compatibility of organic linkers can generate multivariant MOFs, but by far, the diversity of secondary building units (SBUs) in a single metal-organic framework is still limited (no more than two in most cases). Here we report a new double-walled [email protected] metal-organic polyhedral framework () with five types of topologically distinct SBUs and its isoreticular evolution to the [email protected] counterpart (). Both MOFs are the first to be constructed with such high numbers of topologically distinct SBUs as well as topologically distinct nodes, and their formation and evolution provide new insight into SBU's controllability.
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http://dx.doi.org/10.1021/jacs.1c08286DOI Listing
November 2021

Regulation of stem cell fate using nanostructure-mediated physical signals.

Chem Soc Rev 2021 Nov 15;50(22):12828-12872. Epub 2021 Nov 15.

State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China.

One of the major issues in tissue engineering is regulation of stem cell differentiation toward specific lineages. Unlike biological and chemical signals, physical signals with adjustable properties can be applied to stem cells in a timely and localized manner, thus making them a hot topic for research in the fields of biomaterials, tissue engineering, and cell biology. According to the signals sensed by cells, physical signals used for regulating stem cell fate can be classified into six categories: mechanical, light, thermal, electrical, acoustic, and magnetic. In most cases, external macroscopic physical fields cannot be used to modulate stem cell fate, as only the localized physical signals accepted by the surface receptors can regulate stem cell differentiation nanoscale fibrin polysaccharide fibers. However, surface receptors related to certain kinds of physical signals are still unknown. Recently, significant progress has been made in the development of functional materials for energy conversion. Consequently, localized physical fields can be produced by absorbing energy from an external physical field and subsequently releasing another type of localized energy through functional nanostructures. Based on the above concepts, we propose a methodology that can be utilized for stem cell engineering and for the regulation of stem cell fate nanostructure-mediated physical signals. In this review, the combined effect of various approaches and mechanisms of physical signals provides a perspective on stem cell fate promotion by nanostructure-mediated physical signals. We expect that this review will aid the development of remote-controlled and wireless platforms to physically guide stem cell differentiation both and , using optimized stimulation parameters and mechanistic investigations while driving the progress of research in the fields of materials science, cell biology, and clinical research.
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http://dx.doi.org/10.1039/d1cs00572cDOI Listing
November 2021

Polycystic ovary syndrome is an independent risk factor for hypertensive disorders of pregnancy: A systematic review, meta-analysis, and meta-regression.

Endocrine 2021 12 16;74(3):518-529. Epub 2021 Oct 16.

Department of Traditional Chinese Medicine, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510240, China.

Purpose: The risk of hypertensive disorders of pregnancy (HDP) in women with polycystic ovary syndrome (PCOS) is inconsistent in some studies. The aim of this meta-analysis was to examine the evidence regarding the strength of the association between PCOS and HDP.

Methods: PubMed, Web of Science, Embase, and the Cochrane Library were systematically searched to identify observational studies investigating HDP in patients with PCOS. The primary outcome was the pooled odds ratio (OR) of HDP, including pregnancy-induced hypertension (PIH) and pre-eclampsia (PE), in women with PCOS compared with the non-PCOS population.

Results: A total of 30 studies were eligible for meta-analysis. PCOS was associated with a higher risk of HDP (OR 2.02, 95CI% 1.83-2.22), including PIH (OR 1.94, 95CI% 1.70-2.21), and PE (OR 2.07, 95CI% 1.91-2.24). The association remained significant after adjusting for age, body mass index (BMI), and nulliparity (HDP: OR 1.48, 95CI% 1.48-1.60; PIH: OR 1.42, 95%CI 1.29-1.57; PE: OR 2.07, and 95%CI 1.91-2.24). The increased risk of HDP for the PCOS group remained significant in subgroups of BMI, Age, singleton pregnancy, multiple pregnancy, gestational diabetes mellitus (GDM), hyperandrogenism, and nulliparity, while the finding was not observed in subgroups of nonhyperandrogenic and non-GDM. In the meta-regression, BMI contributed significantly to the heterogeneity in the prevalence of HDP.

Conclusions: PCOS is independently associated with a significantly increased risk of HDP. To prevent HDP during pregnancy, our findings highlight the importance of establishing supervision guidelines for PCOS patients, especially in the population with hyperandrogenism and GDM.
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http://dx.doi.org/10.1007/s12020-021-02886-9DOI Listing
December 2021

Secondary instability of the spike-bubble structures induced by nonlinear Rayleigh-Taylor instability with a diffuse interface.

Phys Rev E 2021 Sep;104(3-2):035108

Institute of Applied Physics and Computational Mathematics, Beijing 100088, China and Center for Applied Physics and Technology, Peking University, Beijing 100871, China.

Laminar-turbulent transition in Rayleigh-Taylor (RT) flows usually starts with infinitesimal perturbations, which evolve into the spike-bubble structures in the nonlinear saturation phase. It is well accepted that the emergence and rapid amplification of the small-scale perturbations are attributed to the Kelvin-Helmholtz-type secondary instability due to the high velocity shears induced by the stretch of the spike-bubble structures, however, there has been no quantitative description on such a secondary instability in literature. Moreover, the instability mechanism may not be that simple, because the acceleration or the "rising bubble" effect could also play a role. Therefore, based on the two-dimensional diffuse-interface RT nonlinear flows, the present paper employs the Arnoldi iteration and generalized Rayleigh quotient iteration methods to provide a quantitative study on the secondary instability. Both sinuous and varicose instability modes with high growth rates are observed, all of which are confirmed to be attributed to both the Rayleigh-Taylor and Kelvin-Helmholtz regimes. The former regime dominates the early-time instability due to the "rising bubble" effect, whereas the latter regime becomes more significant as time advances. Being similar to the primary RT instability [Yu et al., Phys. Rev. E 97, 013102 (2018)2470-004510.1103/PhysRevE.97.013102, Dong et al., Phys. Rev. E 99, 013109 (2019)2470-004510.1103/PhysRevE.99.013109, Fan and Dong, Phys. Rev. E 101, 063103 (2020)2470-004510.1103/PhysRevE.101.063103], the diffuse interface also leads to a multiplicity of the secondary instability modes and higher-order modes are found to exhibit more local extremes than the lower-order ones. Direct numerical simulations are carried out, which confirm the linear growth of the secondary instability modes with infinitesimal amplitudes and show their evolution to the turbulent-mixing state.
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http://dx.doi.org/10.1103/PhysRevE.104.035108DOI Listing
September 2021

Hydrogen-bonded silicene nanosheets of engineered bandgap and selective degradability for photodynamic therapy.

Biomaterials 2021 11 4;278:121172. Epub 2021 Oct 4.

State Key Laboratory of High Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, 200050, PR China; Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, PR China. Electronic address:

Silicon, a highly biocompatible and ubiquitous chemical element in living systems, exhibits great potentials in biomedical applications. However, the silicon-based nanomaterials such as silica and porous silicon have been largely limited to only serving as carriers for delivery systems, due to the lack of intrinsic functionalities of silicon. This work presents the facile construction of a two-dimensional (2D) hydrogen-bonded silicene (H-silicene) nanosystem which is highlighted with tunable bandgap and selective degradability for tumor-specific photodynamic therapy facilely by surface covalent modification of hydrogen atoms. Briefly, the H-silicene nanosheet material is selectively degradable in normal neutral tissues but rather stable in the mildly acidic tumor microenvironment (TME) for achieving efficient photodynamic therapy (PDT). Such a 2D hydrogen-bonded silicene nanosystem featuring the tunable bandgap and tumor-selective degradability provides a new paradigm for the application of multi-functional two-dimensional silicon-based biomaterials towards the diagnosis and treatments of cancer and other diseases.
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http://dx.doi.org/10.1016/j.biomaterials.2021.121172DOI Listing
November 2021

Effect of cold and heat therapies on pain relief in patients with delayed onset muscle soreness: A network meta-analysis.

J Rehabil Med 2021 Oct 12. Epub 2021 Oct 12.

.

Objective: To comprehensively compare the effectiveness of cold and heat therapies for delayed onset muscle soreness using network meta-analysis.

Methods: Eight Chinese and English databases were searched from date of establishment of the database to 31 May 2021. Cochrane risk-of-bias tool was used to analyse the included randomized controlled trials. Potential papers were screened for eligibility, and data were extracted by 2 independent researchers.

Results: A total of 59 studies involving 1,367 patients were eligible for this study. Ten interventions were examined: contrast water therapy, phase change material, the novel modality of cryotherapy, cold-water immersion, hot/warm-water immersion, cold pack, hot pack, ice massage, ultrasound, and passive recovery. Network meta-analysis results showed that: (i) within 24 h after exercise, hot pack was the most effective for pain relief, followed by contrast water therapy; (ii) within 48 h, the ranking was hot pack, followed by the novel modality of cryotherapy; and (iii) over 48 h post-exercise, the effect of the novel modality of cryotherapy ranked first.

Conclusion: Due to the limited quality of the included studies, further well-designed research is needed to draw firm conclusions about the effectiveness of cold and heat therapies for delayed onset muscle soreness.
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http://dx.doi.org/10.2340/jrm.v53.331DOI Listing
October 2021

Role of laryngopharyngeal reflux (LPR) in complications after tonsillectomy in adult patients.

Acta Otolaryngol 2021 Oct 10:1-5. Epub 2021 Oct 10.

Department of Otorhinolaryngology Head and Neck Surgery, Peking University People's Hospital, Peking University, Beijing, China.

Background: Tonsillectomy and tonsillectomy with adenoidectomy are among the most common surgical procedures in otolaryngology practice, but the complications, especially the postoperative bleeding, are seriously troubling surgeons. Some authors had identified that gastroesophageal reflux and LPR are both risk factors for complications in tonsillectomy of children patients.

Aims/objectives: Therefore, we designed this prospective study to assess the role of LPR in the development of complications following tonsillectomy in adult patients. We want to provide a basis for future clinical practice.

Material And Methods: We recruited a totally of 150 adult patients (18-60 years old) who had an indication of tonsillectomy because of recurrent tonsillitis and divided them into two groups, the laryngopharyngeal reflux (LPR) group and the control group identified by the results of Reflux Symptom Index (RSI) and Reflux Finding Score (RFS). The complications that were observed and evaluated were pain, hemorrhage, fever, infection, and pulmonary problems.

Result: All the patients complained of pain after surgery. The duration of the pain in the LPR group was much longer than that of the control group. In the LPR group, patients had a similar pain level on the first day after surgery. However, on the 7th day and 14th day after surgery, the pain level was obviously higher than that of the control group. We found that the body temperature mildly rose on the 1st day after surgery and gradually fell to normal level in both groups. No cases had bleeding within 24 h after surgery. There were nine cases of bleeding in the LPR group, while only one case of bleeding occurred in the control group. There were no cases of infection or pulmonary complications in either group.

Conclusions And Significance: LPR is closely related to the complications followed by tonsillectomy and we also suggest that high dosages of proton pump inhibitors (PPI), alkaline water, and alginates should be proscribed to the patients with LPR during the perioperative period.
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http://dx.doi.org/10.1080/00016489.2021.1982148DOI Listing
October 2021

[Analysis of bacterial infection and drug sensitivity in patients with chronic suppurative otitis media].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Oct;35(10):870-874

Department of Otolaryngology,People's Hospital,Peking University,Beijing,100044,China.

To explore the distribution of pathogenic bacteria and drug sensitivity among patients with chronic suppurative otitis media. Patients with chronic suppurative otitis media who were hospitalized in the Department of Otolaryngology, People's Hospital, Peking University for surgery from January 1, 2019 to May 1, 2021 were enrolled as the subjects, then take the deep secretions of the external auditory canal for bacterial culture. Finally, the distribution and drug sensitivity of the pathogenic bacteria are analyzed. A total of 126 patients were enrolled, of which 53 were culture-positive, and 57 strains of bacteria were isolated, including 47 strains of Gram-positive cocci, 10 strains of Gram-negative bacilli. Among Gram-positive cocci, 24 methicillin-sensitive staphylococcus aureus(MSSA) strains, 7 methicillin-resistant staphylococcus aureus(MRSA) strains, 14 coagulase-negative staphylococcus strains, 1 strain enterococcus faecium, and 1 strain otitis Zurich. 4 strains(4/10) of pseudomonas aeruginosa among Gram-negative bacilli. Among them, MSSA has a high resistance rate to levofloxacin, moxifloxacin, clindamycin, and gentamicin, and high sensitivity to oxacillin, vancomycin, linezolid, and rifampin. MRSA is highly resistant to common antibiotics except vancomycin, rifampicin, and linezolid. The Gram-negative bacilli have higher resistance rates to levofloxacin, aztreonam, ciprofloxacin, ceftazidime, and piperacillin/tazobactam, and to meropenem, imipenem, amika star, cefepoxime, cefoperazone/sulbactam, and tobramycin are more sensitive. The results of univariable and multivariable analysis showed that age and bacterial infection were independent factors related to dry ears, and the duration of the disease history was a non-independent factor. The main pathogenic bacteria infections in patients with chronic suppurative otitis media are MSSA, MRSA, and pseudomonas aeruginosa. Whether it is combined with bacterial infection and age are independent factors related to whether patients with chronic suppurative otitis media could have dry ears. Therefore, patients with chronic suppurative otitis media should be treated with specific antibiotics or surgical interventions as soon as possible in combination with drug sensitivity results to avoid prolonging disease course or causing serious infectious complications.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.10.002DOI Listing
October 2021

Predicting cognitive impairment in outpatients with epilepsy using machine learning techniques.

Sci Rep 2021 Oct 8;11(1):20002. Epub 2021 Oct 8.

Department of Neurology, Fujian Medical University Union Hospital, Fujian, People's Republic of China.

Many studies report predictions for cognitive function but there are few predictions in epileptic patients; therefore, we established a workflow to efficiently predict outcomes of both the Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) in outpatients with epilepsy. Data from 441 outpatients with epilepsy were included; of these, 433 patients met the 12 clinical characteristic criteria and were divided into training (n = 304) and experimental (n = 129) groups. After descriptive statistics were analyzed, cross-validation was used to select the optimal model. The random forest (RF) algorithm was combined with the redundancy analysis (RDA) algorithm; then, optimal feature selection and resampling were carried out after removing linear redundancy information. The features that contributed more to multiple outcomes were selected. Finally, the external traceability of the model was evaluated using the follow-up data. The RF algorithm was the best prediction model for both MMSE and MoCA outcomes. Finally, seven markers were screened by overlapping the top ten important features for MMSE ranked by RF modeling, those ranked for MoCA ranked by RF modeling, and those for both assessments ranked by RDA. The optimal combination of features were namely, sex, age, age of onset, seizure frequency, brain MRI abnormalities, epileptiform discharge in EEG and usage of drugs. which was the most efficient in predicting outcomes of MMSE, MoCA, and both assessments.
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http://dx.doi.org/10.1038/s41598-021-99506-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501137PMC
October 2021

Community and Academic Physicians Working Together in Integrated Health Care Systems.

Mayo Clin Proc Innov Qual Outcomes 2021 Oct 21;5(5):951-960. Epub 2021 Sep 21.

Department of Anesthesia, Roy J. & Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA, USA.

Objective: To examine best practices and policies for effectively merging community and academic physicians in integrated health care systems.

Methods: Deans of US allopathic medical schools were systematically interviewed between February and June 2017 regarding growth in their faculty practice plan (FPP), including logistics and best practices for integration of community physicians.

Results: The survey was completed by 107 of 143 (74.8) of US medical school deans approached. Of these institutions, 73 met criteria for final analysis (research-based medical schools with FPPs of >300 physicians). Most academic medical center-based FPPs have increased in size over the last 5 years, with further growth anticipated via adding community physicians (85%). Because of disparate practice locations, integration of community and academic physicians has been slow. When fully integrated, community physicians predominantly have a clinical role with productivity incentives. Deans report that cultural issues must be addressed to avoid conflict. Consensus exists that transparent clinical work requirements for all FPP members, clearly defined productivity incentives, additional promotion tracks, and early involvement of department chairs and other leaders enhances trust and creates better synergy among all physician providers.

Conclusion: Findings from this study should help guide FPPs, academic medical center leaders, chief medical officers, and professional and trade organizations in working toward positive physician synergy in consolidated health care organizations. Work and cultural considerations must be addressed to honor distinct talents of each physician group, facilitating smooth transition from disparate groups to healthy synergy.
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http://dx.doi.org/10.1016/j.mayocpiqo.2021.06.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473661PMC
October 2021

5-HT Receptor Is Involved in Electroacupuncture Inhibition of Chronic Pain in the Spinal Cord.

Front Neurosci 2021 17;15:733779. Epub 2021 Sep 17.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Institute of Neuroscience, Translational Medicine Institute, Xi'an Jiaotong University Health Science Center, Xi'an, China.

Knee osteoarthritis (KOA) is a common and disabling condition characterized by attacks of pain around the joints, and it is a typical disease that develops chronic pain. Previous studies have proved that 5-HT, 5-HT, and 5-HT receptors in the spinal cord are involved in electroacupuncture (EA) analgesia. The 5-HT receptor plays antinociceptive role in the spinal cord. However, it is unclear whether the 5-HT receptor is involved in EA analgesia. The 5-HT receptor is a stimulatory G-protein (Gs)-coupled receptor that activates adenylyl cyclase (AC) to stimulate cyclic adenosine monophosphate (cAMP) formation, which in turn activates protein kinase A (PKA). In the present study, we found that EA significantly increased the tactile threshold and the expression of the 5-HT receptor in the dorsal spinal cord. Intrathecal injection of 5-HT receptor agonist AS-19 mimicked the analgesic effect of EA, while a selective 5-HT receptor antagonist reversed this effect. Moreover, intrathecal injection of AC and PKA antagonists prior to EA intervention prevented its anti-allodynic effect. In addition, GABA receptor antagonist bicuculline administered (intrathecal, i.t.) prior to EA intervention blocked the EA effect on pain hypersensitivity. Our data suggest that the spinal 5-HT receptor activates GABAergic neurons through the Gs-cAMP-PKA pathway and participates in EA-mediated inhibition of chronic pain in a mouse model of KOA.
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http://dx.doi.org/10.3389/fnins.2021.733779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484641PMC
September 2021

Decrease in Serum Urate Level Is Associated With Loss of Visceral Fat in Male Gout Patients.

Front Endocrinol (Lausanne) 2021 14;12:724822. Epub 2021 Sep 14.

Shandong Provincial Key Laboratory of Metabolic Diseases and Qingdao Key Laboratory of Gout, The Affiliated Hospital of Qingdao University, Qingdao, China.

Objective: To clarify the relationship between serum urate (SU) decrease and visceral fat area (VFA) reduction in patients with gout.

Methods: We retrospectively analyzed 237 male gout patients who had two sets of body composition and metabolic measurements within 6 months. Subjects included had all been treated with urate-lowering therapy (ULT) (febuxostat 20-80 mg/day or benzbromarone 25-50 mg/day, validated by the medical record). All patients were from the specialty gout clinic of The Affiliated Hospital of Qingdao University. The multiple linear regression model evaluated the relationship between change in SU [ΔSU, (baseline SU) - (final visit SU)] and change in VFA [ΔVFA, (baseline VFA) - (final visit VFA)].

Results: ULT resulted in a mean (standard deviation) decrease in SU level (464.22 ± 110.21 μmol/L at baseline, 360.93 ± 91.66 μmol/L at the final visit, 0.001) accompanied by a decrease in median (interquartile range) VFA [97.30 (81.15-118.55) at baseline, 90.90 (75.85-110.05) at the final visit, p < 0.001]. By multiple regression model, ΔSU was identified to be a significant determinant variable of decrease in VFA (beta, 0.302; = 0.001).

Conclusions: The decrease in SU level is positively associated with reduced VFA. This finding provides a rationale for clinical trials to affirm whether ULT promotes loss of visceral fat in patients with gout.
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http://dx.doi.org/10.3389/fendo.2021.724822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476917PMC
September 2021
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