Publications by authors named "Lin Gao"

545 Publications

Intracavity generation of glioma stem cell-specific CAR macrophages primes locoregional immunity for postoperative glioblastoma therapy.

Sci Transl Med 2022 Aug 3;14(656):eabn1128. Epub 2022 Aug 3.

Key Laboratory of Chemical Biology (Ministry of Education), Department of Pharmaceutics, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, 44 Cultural West Road, Jinan, Shandong Province 250012, China.

Glioblastoma multiforme (GBM) remains incurable despite aggressive implementation of multimodal treatments after surgical debulking. Almost all patients with GBM relapse within a narrow margin around the initial resected lesion due to postsurgery residual glioma stem cells (GSCs). Tracking and eradicating postsurgery residual GSCs is critical for preventing postoperative relapse of this devastating disease, yet effective strategies remain elusive. Here, we report a cavity-injectable nanoporter-hydrogel superstructure that creates GSC-specific chimeric antigen receptor (CAR) macrophages/microglia (MΦs) surrounding the cavity to prevent GBM relapse. Specifically, we demonstrate that the CAR gene-laden nanoporter in the hydrogel can introduce GSC-targeted CAR genes into MΦ nuclei after intracavity delivery to generate CAR-MΦs in mouse models of GBM. These CAR-MΦs were able to seek and engulf GSCs and clear residual GSCs by stimulating an adaptive antitumor immune response in the tumor microenvironment and prevented postoperative glioma relapse by inducing long-term antitumor immunity in mice. In an orthotopic patient-derived glioblastoma humanized mouse model, the combined treatment with nanoporter-hydrogel superstructure and CD47 antibody increased the frequency of positive immune responding cells and suppressed the negative immune regulating cells, conferring a robust tumoricidal immunity surrounding the postsurgical cavity and inhibiting postoperative glioblastoma relapse. Therefore, our work establishes a locoregional treatment strategy for priming cancer stem cell-specific tumoricidal immunity with broad application in patients suffering from recurrent malignancies.
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http://dx.doi.org/10.1126/scitranslmed.abn1128DOI Listing
August 2022

Epidemiological investigation and phylogenetic analysis of Classical Swine Fever virus in Yunnan province from 2015 to 2021.

J Vet Sci 2022 Jul;23(4):e57

Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science & Veterinary Institute, Kunming 650224, China.

Background: Classical swine fever virus (CSFV), the causative agent of classical swine fever (CFS), is a highly contagious disease that poses a serious threat to Chinese pig populations.

Objectives: Many provinces of China, such as Shandong, Henan, Hebei, Heilongjiang, and Liaoning provinces, have reported epidemics of CSFV, while the references to the epidemic of CSFV in Yunnan province are rare. This study examined the epidemic characteristics of the CSFV in Yunnan province.

Methods: In this study, 326 tissue samples were collected from different regions in Yunnan province from 2015 to 2021. A reverse transcription-polymerase chain reaction (RT-PCR), sequences analysis, and phylogenetic analysis were performed for the pathogenic detection and analysis of these 326 clinical specimens.

Results: Approximately 3.37% (11/326) of specimens tested positive for the CSFV by RT-PCR, which is lower than that of other regions of China. Sequence analysis of the partial E2 sequences of eleven CSFV strains showed that they shared 89.0-100.0% nucleotide (nt) and 95.0-100.0% amino acid (aa) homology, respectively. Phylogenetic analysis showed that these novel isolates belonged to the subgenotypes 2.1c and 2.1d, with subgenotype 2.1c being predominant.

Conclusions: The CSFV was sporadic in China's Yunnan province from 2015 to 2021. Both 2.1c and 2.1d subgenotypes were found in this region, but 2.1c was dominant.
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http://dx.doi.org/10.4142/jvs.22042DOI Listing
July 2022

Posterior Surgery in the Treatment of Craniovertebral Junction Deformity with Torticollis.

Orthop Surg 2022 Aug 1. Epub 2022 Aug 1.

Department of Spine Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an City, China.

Objective: To investigate the clinical effect of posterior surgery in the treatment of craniovertebral junction (CVJ) deformities with torticollis and methods for preventing and treating complications in order to obtain a reasonable treatment strategy.

Methods: From January 2007 to December 2017, 78 patients who suffered from CVJ deformities with torticollis treated by posterior surgery were analyzed. The surgical techniques were all posterior correction and fusion to restore the anatomical alignment of the craniovertebral junction. The visual analog score (VAS) and Short Form-36 (SF-36) health survey questionnaire were utilized to evaluate preoperative and postoperative neck pain, and changes in the torticollis angle and atlas-dens interval (ADI) were evaluated through anteroposterior X-ray and computed tomography. Intra- and postoperative complications were all recorded. One-way ANOVA, LSD-t test, and χ test were performed to evaluate the difference between the preoperative and postoperative data.

Results: The mean follow-up time was 37.4 ± 15.7 months, the average operation time was 115.6 ± 12.8 min, and the average blood loss was 170.8 ± 26.3 mL. According to the deformity site, the range of posterior correction and fusion was as follows: 38 cases of C -C , 33 cases of C -C , and seven cases of C -C . The preoperative SF-36, VAS, torticollis angle, and ADI were 42.6 ± 8.8, 4.8 ± 1.1, 37.2 ± 11.2°, and 4.9 ± 2.3 mm, respectively. The difference was significant at 3 months post operation (p < 0.05), and there was no significant difference at the final follow-up compared with 3 months post operation (p > 0.05).

Conclusion: It can objectively achieve favorable correction and satisfactory clinical effects under posterior correction and fixation for CVJ deformities with torticollis. Intra- and postoperative complications can be settled by proper management.
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http://dx.doi.org/10.1111/os.13324DOI Listing
August 2022

Multidrug Representation Learning Based on Pretraining Model and Molecular Graph for Drug Interaction and Combination Prediction.

Bioinformatics 2022 Jul 29. Epub 2022 Jul 29.

School of Computer Science and Technology, Xidian University, Xi'an 710071, Shaanxi, China.

Motivation: Approaches for the diagnosis and treatment of diseases often adopt multidrug therapy method because it can increase the efficacy or reduce the toxic side effects of drugs. Using different drugs simultaneously may trigger unexpected pharmacological effects. Therefore, efficient identification of drug interactions is essential for the treatment of complex diseases. Currently proposed calculation methods are often limited by the collection of redundant drug features, a small amount of labeled data, and low model generalization capabilities. Meanwhile, there is also a lack of unique methods for multidrug representation learning, which makes it more difficult to take full advantage of the originally scarce data.

Results: Inspired by graph models and pretraining models, we integrated a large amount of unlabeled drug molecular graph information and target information, then designed a pretraining framework, MGP-DR (Molecular Graph Pretraining for Drug Representation), specifically for drug pair representation learning. The model uses self-supervised learning strategies to mine the contextual information within and between drug molecules to predict drug-drug interactions and drug combinations. The results achieved promising performance across multiple metrics compared with other state-of-the-art methods. Our MGP-DR model can be used to provide a reliable candidate set for the combined use of multiple drugs.

Availability And Implementation: Code of the model, datasets and results can be downloaded from GitHub (https://github.com/LiangYu-Xidian/MGP-DR).

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btac538DOI Listing
July 2022

Blue light-induced phosphorylation of Arabidopsis CRY1 is essential for its photosensitivity.

J Integr Plant Biol 2022 Jul 27. Epub 2022 Jul 27.

Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Plants possess two cryptochrome photoreceptors, cryptochrome 1 (CRY1) and cryptochrome 2 (CRY2), that mediate overlapping and distinct physiological responses. Both CRY1 and CRY2 undergo blue light-induced phosphorylation, but the molecular details of CRY1 phosphorylation remain unclear. Here we identify 19 in vivo phosphorylation sites in CRY1 by mass spectrometry and systematically analyze the physiological and photobiochemical activities of CRY1 variants with phosphosite substitutions. We demonstrate that nonphosphorylatable CRY1 variants have impaired phosphorylation, degradation, and physiological functions, whereas phosphomimetic variants mimic the physiological functions of phosphorylated CRY1 to constitutively inhibit hypocotyl elongation. We further demonstrate that phosphomimetic CRY1 variants exhibit enhanced interaction with the E3 ubiquitin ligase COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC 1). This finding is consistent with the hypothesis that phosphorylation of CRY1 is required for COP1-dependent signaling and regulation of CRY1. We also determine that PHOTOREGULATORY PROTEIN KINASEs (PPKs) phosphorylate CRY1 in a blue light-dependent manner and that this phosphorylation is critical for CRY1 signaling and regulation. These results indicate that, similar to CRY2, blue light-dependent phosphorylation of CRY1 determines its photosensitivity. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/jipb.13331DOI Listing
July 2022

Conserved Control Path in Multilayer Networks.

Entropy (Basel) 2022 Jul 15;24(7). Epub 2022 Jul 15.

School of Computer Science and Technology, Xidian University, Xi'an 710071, China.

The determination of directed control paths in complex networks is important because control paths indicate the structure of the propagation of control signals through edges. A challenging problem is to identify them in complex networked systems characterized by different types of interactions that form multilayer networks. In this study, we describe a graph pattern called the conserved control path, which allows us to model a common control structure among different types of relations. We present a practical conserved control path detection method (CoPath), which is based on a maximum-weighted matching, to determine the paths that play the most consistent roles in controlling signal transmission in multilayer networks. As a pragmatic application, we demonstrate that the control paths detected in a multilayered pan-cancer network are statistically more consistent. Additionally, they lead to the effective identification of drug targets, thereby demonstrating their power in predicting key pathways that influence multiple cancers.
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http://dx.doi.org/10.3390/e24070979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324794PMC
July 2022

The Androgen Receptor and Its Crosstalk With the Src Kinase During Castrate-Resistant Prostate Cancer Progression.

Front Oncol 2022 27;12:905398. Epub 2022 Jun 27.

Department of Urologic Sciences, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada.

While the androgen receptor (AR) signalling is the mainstay therapeutic target for metastatic prostate cancers, these tumours will inevitably develop therapy resistance to AR pathway inhibitors suggesting that prostate tumour cells possess the capability to develop mechanisms to bypass their dependency on androgens and/or AR to survive and progress. In many studies, protein kinases such as Src are reported to promote prostate tumour progression. Specifically, the pro-oncogene tyrosine Src kinase regulates prostate cancer cell proliferation, adhesion, invasion, and metastasis. Not only can Src be activated under androgen depletion, low androgen, and supraphysiological androgen conditions, but also through crosstalk with other oncogenic pathways. Reciprocal activations between Src and AR proteins had also been reported. These findings rationalize Src inhibitors to be used to treat castrate-resistant prostate tumours. Although several Src inhibitors had advanced to clinical trials, the failure to observe patient benefits from these studies suggests that further evaluation of the roles of Src in prostate tumours is required. Here, we summarize the interplay between Src and AR signalling during castrate-resistant prostate cancer progression to provide insights on possible approaches to treat prostate cancer patients.
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http://dx.doi.org/10.3389/fonc.2022.905398DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271573PMC
June 2022

Realization of Music-Assisted Interactive Teaching System Based on Virtual Reality Technology.

Authors:
Yan Gao Lin Gao

Occup Ther Int 2022 11;2022:1007954. Epub 2022 Jun 11.

University of South Seoul, Cheonan 3102091, Republic of Korea.

Virtual reality technology has attracted researchers' attention because it can provide users with a virtual interactive learning environment. Based on the theory of virtual reality technology, this paper proposes the system model design and architecture of virtual interactive music-assisted interactive teaching and realizes key technologies such as modeling, music-assisted interactive teaching scene interaction, and database access. In the simulation process, based on the VRML/X3D bottom interactive system template, after comprehensive application research, comparative analysis of various modeling methods, the system verified the use of digital cameras combined with the modeling technology based on music elements to collaboratively establish VRML virtual model connections. For inline node function, we combined it with Outline3D to realize VRML integration and then use VizX3D, X3D-Edit to build X3D model and realize the conversion from VRML to X3D, which solves the system completeness problem of music-assisted interactive teaching. The experimental results show that, according to the statistical analysis of the data after the experiment, when the position changes in the virtual 3D music-assisted interactive teaching scene, it will be displayed in the plane layer, and the real-time coordinates of the virtual music-assisted interactive teaching scene displayed in HTML have case. By analyzing the scenes and dynamic effects in the works, the effects of the virtual world can be better displayed through the performance of details. The better accuracy and delay error reached 89.7% and 3.11%, respectively, which effectively improved the effect and feasibility of applying virtual reality technology to music-assisted interactive teaching.
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http://dx.doi.org/10.1155/2022/1007954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206570PMC
July 2022

Yin Yang 1 promotes aggressive cell growth in high-grade breast cancer by directly transactivating kinectin 1.

MedComm (2020) 2022 Sep 5;3(3):e133. Epub 2022 Jul 5.

Department of Clinical Medical Research Center The Second Clinical Medical College Jinan University (Shenzhen People's Hospital) The First Affiliated Hospital of Southern University of Science and Technology Shenzhen Guangdong China.

Invasive cancer growth and metastasis account for the poor prognosis of high-grade breast cancer. Recently, we reported that kinectin 1 (KTN1), a member of the kinesin-binding protein family, promotes cell invasion of triple-negative breast cancer and high-grade breast cancer cells by augmenting the NF-κB signaling pathway. However, the upstream mechanism regulating KTN1 is unknown. Therefore, this functional study was performed to decipher the regulatory cohort of KTN1 in high-grade breast cancer. Bioinformatic analysis indicated that transcription factor Yin Yang 1 (YY1) was a potential transactivator of KTN1. High YY1 expression correlated positively with pathological progression and poor prognosis of high-grade breast cancer. Additionally, YY1 promoted cell invasive growth both in vitro and in vivo, in a KTN1-dependent manner. Mechanistically, YY1 could transactivate the KTN1 gene promoter. Alternatively, YY1 could directly interact with a co-factor, DEAD-box helicase 3 X-linked (DDX3X), which significantly co-activated YY1-mediated transcriptional expression of KTN1. Moreover, DDX3X augmented YY1-KTN1 signaling-promoted invasive cell growth of breast cancer. Importantly, overexpression of YY1 enhanced tumor aggressive growth in a mouse breast cancer model. Our findings established a novel DDX3X-assisted YY1-KTN1 regulatory axis in breast cancer progression, which could lead to the development novel therapeutic targets for breast cancer.
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http://dx.doi.org/10.1002/mco2.133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253731PMC
September 2022

Genetic profile identification and clinicopathologic characteristics analysis of the thyroid-like low-grade nasopharyngeal papillary adenocarcinoma.

Pathol Res Pract 2022 Aug 16;236:153980. Epub 2022 Jun 16.

Department of Pathology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China. Electronic address:

Thyroid-like low-grade nasopharyngeal papillary adenocarcinoma (TL-LGNPPA) is an extremely rare malignancy bearing histomorphological similarities to papillary thyroid carcinoma with good prognosis. It's important to distinguish TL-LGNPPA from other papillary tumors including nasopharyngeal papillary adenocarcinoma (NPPA), metastatic and ectopic papillary thyroid cancer, and metastasized adenocarcinomas, etc. To date, only 48 cases of TL-LGNPPA have been reported in the English literatures. Here, we reported the genomic characteristics of additional 4 cases and reviewed other reports to clarify the clinicopathological features of this tumor. In this study, 41 mutations were detected by whole-exome sequencing, but no typical driver mutations were found. Two sample with Copy Number Variations (CNV) were found (7 q22. 17 q12), of which the segment spanned the regions of RASA4, POLR2J2, SPDYE2, CCL3, CCL4, etc. Additionally, no MSI and HLA LOH were found. To our knowledge, we are the first to reveal the genetic underpinnings of this rare tumor. The clinicopathological features of TL-LGNPPA were characterized, shedding more light on the essential difference between TL-LGNPPA with other papillary tumors.
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http://dx.doi.org/10.1016/j.prp.2022.153980DOI Listing
August 2022

Ethylene Promotes Expression of the Appressorium- and Pathogenicity-Related Genes via GPCR- and MAPK-Dependent Manners in .

J Fungi (Basel) 2022 May 26;8(6). Epub 2022 May 26.

School of Life Sciences, East China Normal University, Shanghai 200241, China.

Ethylene (ET) represents a signal that can be sensed by plant pathogenic fungi to accelerate their spore germination and subsequent infection. However, the molecular mechanisms of responses to ET in fungi remain largely unclear. In this study, was investigated via transcriptomic analysis to reveal the genes that account for the ET-regulated fungal development and virulence. The results showed that ET promoted genes encoding for fungal melanin biosynthesis enzymes, extracellular hydrolases, and appressorium-associated structure proteins at 4 h after treatment. When the germination lasted until 24 h, ET induced multiple appressoria from every single spore, but downregulated most of the genes. Loss of selected ET responsive genes encoding for scytalone dehydratase () and cerato-platanin virulence protein () were unable to alter ET sensitivity of in vitro but attenuated the influence of ET on pathogenicity. Knockout of the G-protein-coupled receptors CgGPCR3-1/2 and the MAPK signaling pathway components CgMK1 and CgSte11 resulted in reduced ET sensitivity. Taken together, this study in reports that ET can cause transcription changes in a large set of genes, which are mainly responsible for appressorium development and virulence expression, and these processes are dependent on the GPCR and MAPK pathways.
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http://dx.doi.org/10.3390/jof8060570DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224669PMC
May 2022

Naringenin regulates gut microbiota and SIRT1/ PGC-1ɑ signaling pathway in rats with letrozole-induced polycystic ovary syndrome.

Biomed Pharmacother 2022 Jun 17;153:113286. Epub 2022 Jun 17.

School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China. Electronic address:

Purpose: To evaluate the effect of naringenin on improving PCOS and explore the mechanism.

Methods: Firstly, we carried out differential gene expression analysis from transcriptome sequencing data of human oocyte to screen the KEGG pathway, then the PCOS-like rat model was induced by letrozole. They were randomly divided into four groups: Normal group (N), PCOS group (P), Diane-35 group (D), and Naringenin group (Nar). The changes of estrus cycle, body weight, ovarian function, serum hormone levels, glucose metabolism, along with the expression of SIRT1, PGC-1ɑ, claudin-1 and occludin of the ovary and colon were investigated. Furthermore, the composition of the gut microbiome of fecal was tested.

Results: By searching the KEGG pathway in target genes, we found that at least 15 KEGG pathways are significantly enriched in the ovarian function, such as AMPK signaling pathway, insulin secretion, and ovarian steroidogenesis. Interestingly, naringenin supplementation significantly reduced body weight, ameliorated hormone levels, improved insulin resistance, and mitigated pathological changes in ovarian tissue, up-regulated the expression of PGC-1ɑ, SIRT1, occludin and claudin-1 in colon. In addition, we also found that the abundance of Prevotella and Gemella was down-regulated, while the abundance of Butyricimonas, Lachnospira, Parabacteroides, Butyricicoccus, Streptococcus, Coprococcus was up-regulated.

Conclusion: Our data suggest that naringenin exerts a treatment PCOS effect, which may be related to the modulation of the gut microbiota and SIRT1/PGC-1ɑ signaling pathway. Our research may provide a new perspective for the treatment of PCOS and related diseases.
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http://dx.doi.org/10.1016/j.biopha.2022.113286DOI Listing
June 2022

Peroxiredoxin 4 Interacts With Domeless and Participates in Antibacterial Immune Response Through the JAK/STAT Pathway.

Front Immunol 2022 26;13:907183. Epub 2022 May 26.

Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, China.

The JAK/STAT pathway plays an important role in the development and immune responses of animals. In vertebrates, families of cytokines or growth factors act as activators of the JAK/STAT pathway; however, the activators for the JAK/STAT signaling pathway in arthropods are largely unknown. Herein we report a new ligand, peroxiredoxin 4 (Prx4), for the Domeless in the JAK/STAT pathway of shrimp . Prx4 was induced to secrete into the extracellular surroundings upon challenge, which then facilitated the anti- activity of shrimp by activating the phosphorylation and nuclear translocation of STAT and the expression of STAT-responsive antimicrobial peptides. Blocking the expression of Prx4 abrogated the activation of the JAK/STAT pathway by infection, while injection of Prx4 protein activated the pathway. The interaction between Prx4 and Domeless was proved by immuno-precipitation and protein pull-down assays. Moreover, two cysteine residues in Prx4 that are critical for the interaction and Prx4's anti- role were identified, and the binding site in Domeless for Prx4 was proved to be the cytokine-binding homology module fragment. Taken together, our study revealed a new function for Prx4 enzyme and established a new enzyme-type ligand for the activation of the JAK/STAT pathway in an aquatic arthropod.
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http://dx.doi.org/10.3389/fimmu.2022.907183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195186PMC
May 2022

Differential responses of bloom-forming Ulva intestinalis and economically important Gracilariopsis lemaneiformis to marine heatwaves under changing nitrate conditions.

Sci Total Environ 2022 Sep 7;840:156591. Epub 2022 Jun 7.

State Key Laboratory of Marine Environmental Science & College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China. Electronic address:

Marine heatwaves (MHWs) are affecting the survival of macroalgae. However, little is known regarding how the impacts of MHWs are regulated by nitrogen availability. In this study, we investigated the physiological and genetic responses of a green-tide macroalga Ulva intestinalis Linnaeus and a commercially cultivated macroalga Gracilariopsis lemaneiformis (Bory) E.Y. Dawson, Acleto & Foldvik under different nitrate conditions to simulated MHWs. Under nitrogen limited conditions (LN), heatwaves did not significantly affect biomass or Fv/Fm of U. intestinalis although it led to an earlier biomass decline due to more reproduction events, and meanwhile an upregulation in genes related to TCA cycle and oxidative phosphorylation was detected, supporting sporulation. Under nitrogen replete conditions (HN), heatwaves did not change biomass, Fv/Fm or photosynthetic pigments but reduced reproduction rate along with insignificant change of oxidative phosphorylation and TCA cycle related genes. Meanwhile, genes related to photosynthesis and glutathione metabolism were upregulated. Regarding G. lemaneiformis, heatwaves reduced its Fv/Fm and photosynthetic pigments content, leading to bleaching and death, and photosynthesis-related genes were also downregulated at LN. Fv/Fm was improved and photosynthesis-related genes were up-regulated by the combination of nitrogen enrichment and heatwaves, whereas G. lemaneiformis remained bleached and died by day 12. Therefore, U. intestinalis could survive heatwaves through shifting to micropropagules at LN and protecting its photosynthesis at HN. In contrast, G. lemaneiformis died of bleaching when suffering heatwaves regardless of nitrogen availability. These findings suggest that in future oceans with eutrophication and MHWs, the harmful alga U. intestinalis may have more advantages over the economic alga G. lemaneiformis.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156591DOI Listing
September 2022

DrawingInStyles: Portrait Image Generation and Editing with Spatially Conditioned StyleGAN.

IEEE Trans Vis Comput Graph 2022 May 30;PP. Epub 2022 May 30.

The research topic of sketch-to-portrait generation has witnessed a boost of progress with deep learning techniques. The recently proposed StyleGAN architectures achieve state-of-the-art generation ability but the original StyleGAN is not friendly for sketch-based creation due to its unconditional generation nature. To address this issue, we propose a direct conditioning strategy to better preserve the spatial information under the StyleGAN framework. Specifically, we introduce Spatially Conditioned StyleGAN (SC-StyleGAN for short), which explicitly injects spatial constraints to the original StyleGAN generation process. We explore two input modalities, sketches and semantic maps, which together allow users to express desired generation results more precisely and easily. Based on SC-StyleGAN, we present DrawingInStyles, a novel drawing interface for non-professional users to easily produce high-quality, photo-realistic face images with precise control, either from scratch or editing existing ones. Qualitative and quantitative evaluations show the superior generation ability of our method to existing and alternative solutions. The usability and expressiveness of our system are confirmed by a user study.
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http://dx.doi.org/10.1109/TVCG.2022.3178734DOI Listing
May 2022

Epidemiological Investigation and Genetic Analysis of Pseudorabies Virus in Yunnan Province of China from 2017 to 2021.

Viruses 2022 04 25;14(5). Epub 2022 Apr 25.

Yunnan Sino-Science Gene Technology Co., Ltd., Kunming 650501, China.

In recent years, the prevalence of pseudorabies virus (PRV) has caused huge economic losses to the Chinese pig industry. Meanwhile, PRV infection in humans also sounded the alarm about its cross-species transmission from pigs to humans. To study the regional PRV epidemic, serological and epidemiological investigations of PRV in pig populations from Yunnan Province during 2017-2021 were performed. The results showed that 31.37% (6324/20,158, 95% CI 30.73-32.01) of serum samples were positive for PRV glycoprotein E (gE)-specific antibodies via enzyme-linked immunosorbent assay (ELISA). The risk factors, including the breeding scale and development stage, were significantly associated with PRV seroprevalence among pigs in Yunnan Province. Of the 416 tissue samples collected from PRV-suspected pigs in Yunnan Province, 43 (10.33%, 95% CI 7.41-13.26) samples were positive for PRV- nucleic acid in which 15 novel PRV strains from these PRV-positive samples were isolated, whose and sequences were analyzed. Phylogenetic analysis showed that all 15 isolates obtained in this study belonged to the genotype II. Additionally, the gene of one isolate (YuN-YL-2017) was genetically closer to variant PRV strains compared with others, while the gene was in the same clade with other classical PRV strains, indicating that this isolate might be a recombinant strain generated from the classical and variant strains. The results revealed the severe PRV epidemic in Yunnan Province and indicated that PRV variants are the major genotypes threatening the pig industry development.
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http://dx.doi.org/10.3390/v14050895DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146497PMC
April 2022

Properties of curcumin-loaded zein-tea saponin nanoparticles prepared by antisolvent co-precipitation and precipitation.

Food Chem 2022 Oct 16;391:133224. Epub 2022 May 16.

College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, People's Republic of China; Qingdao Special Food Research Institute, Qingdao 266109, People's Republic of China. Electronic address:

The properties of nutraceutical-loaded biopolymer nanoparticles fabricated by antisolvent co-precipitation (ASCP) and precipitation (ASP) were compared. Curcumin-loaded zein-tea saponin nanoparticles were fabricated using both methods and then their structural and physicochemical properties were characterized. The diameter of the nanoparticles prepared by ASCP were smaller (120-130 nm) than those prepared by ASP (140-160 nm). The encapsulation efficiency of the ASCP-nanoparticles (80.0%) was higher than the ASP-ones (71.0%) at a zein-to-curcumin mass ratio of 3:1, which was also higher than previous studies. The storage and light stability of curcumin was higher in zein-saponin nanoparticles than in zein nanoparticles. All nanoparticles had good water dispersibility after freeze-drying and rehydration. This study shows that nanoparticles produced by antisolvent co-precipitation have superior properties to those produced by antisolvent precipitation. The co-precipitation method leads to a higher encapsulation efficiency, smaller particle size, and greater storage stability, which may be advantageous for some applications.
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http://dx.doi.org/10.1016/j.foodchem.2022.133224DOI Listing
October 2022

Confining ZnS/SnS Ultrathin Heterostructured Nanosheets in Hollow N-Doped Carbon Nanocubes as Novel Sulfur Host for Advanced Li-S Batteries.

Small 2022 Jun 17;18(24):e2107727. Epub 2022 May 17.

Collaborative Innovation Center for Microgrid of New Energy, College of Electrical Engineering & New Energy, China Three Gorges University, Yichang, 443002, P. R. China.

Hollow nanostructured hosts are important scaffolds to achieve high sulfur loading, fast charge transfer, and conspicuous restraint of lithium polysulfides (LiPSs) shuttling in lithium-sulfur (Li-S) batteries. However, developing high-efficiency hollow hosts for improving utilization and conversion of aggregated sulfur in the hollow chamber remains a longstanding challenge. Herein, hollow N-doped carbon nanocubes confined petal-like ZnS/SnS heterostructures (ZnS/SnS @NC) as a conceptually novel host for Li-S batteries are reported. Specifically, compared to consubstantial hollow double-shelled hosts, the ZnS/SnS @NC with higher effective active surface area brings dense contact with sulfur and enhances efficient adsorption sites for binding LiPSs and accelerating their conversion. Benefiting from the unique structure and sophisticated composition, the resulting [email protected]/SnS @NC cathodes exhibit 1294 mAh g at 0.2 C, an ultralow capacity decay of 0.016% per cycle over 500 cycles at 1.0 C, and a high area capacity of 4.77 mAh cm at 0.5 C (5.9 mg cm ). Meanwhile, the performance evolution of [email protected]/SnS @NC cathodes under various sulfur loadings is further investigated by using EIS, which provides the beneficial guidance to explore viable strategies further optimizing their performance. This work sheds new insights into the design of hollow nanostructured hosts with a distinguished ability to regulate LiPSs in Li-S batteries.
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http://dx.doi.org/10.1002/smll.202107727DOI Listing
June 2022

Risk factors for proximal junctional kyphosis after posterior long-segment internal fixation for chronic symptomatic osteoporotic thoracolumbar fractures with kyphosis.

BMC Surg 2022 May 14;22(1):189. Epub 2022 May 14.

Department of Spine Surgery, Honghui Hospital, Xi'an Jiaotong University, No.555, Youyi East Road, Xi'an, Shaanxi, China.

Background: This study aimed to analyze the risk factors for proximal junctional kyphosis (PJK) for patients with chronic symptomatic osteoporotic thoracolumbar fractures (CSOTLF) and kyphosis who underwent long-segment internal fixation.

Methods: We retrospectively reviewed the records of patients with CSOTLF complicated with kyphosis who underwent posterior multilevel internal fixation in our hospital between January 2013 and January 2020. The patients' age, sex, body mass index (BMI), bone mineral density (BMD), smoking status, cause of injury, comorbidities, injury segments, and American Spinal Injury Association (ASIA) grading non-surgical data; posterior ligament complex (PLC) injury, upper and lower instrumented vertebral position (UIV and LIV, respectively), number of fixed segments surgical data, proximal junctional angle (PJA), sagittal vertebral axis (SVA), pelvic incidence (PI), lumbar lordosis (LL), pelvic incidence-lumbar lordosis mismatch (PI-LL), pelvic tilt (PT), and sacral slope (SS) surgical indicators were collected. Patients were divided into postoperative PJK and non-PJK groups.

Results: This study included 90 patients; among them, 30 (31.58%) developed PJK postoperatively. All patients were followed up for > 24 months (mean 32.5 months). Univariate analysis showed significant differences in age, BMI, BMD, PLC injury, UIV, and LIV fixation position, number of fixation stages, and preoperative PJA, SVA, PI-LL, and SS between the two groups (P < 0.05). Additionally, no significant differences were observed in sex, smoking, cause of injury, complications, injury segment ASIA grade, and preoperative PT between the two groups (P > 0.05). Multifactorial logistic regression analysis showed that age > 70 years (OR = 32.279, P < 0.05), BMI > 28 kg/m (OR = 7.876, P < 0.05), BMD T value < - 3.5 SD (OR = 20.836, P < 0.05), PLC injury (OR = 13.981, P < 0.05), and preoperative PI-LL > 20° (OR = 13.301, P < 0.05) were risk factors for PJK after posterior long-segment internal fixation in elderly patients with CSOTLF complicated with kyphosis.

Conclusion: CSOTLF patients undergoing posterior long segment internal fixation are prone to PJK, and age > 70 years, BMI > 28 kg/m2, BMD T value < - 3.5 SD, preoperative PI-LL > 20° and PLC injury may increase their risk.
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http://dx.doi.org/10.1186/s12893-022-01598-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107743PMC
May 2022

Case series of unilateral biportal endoscopic-assisted transforaminal lumbar interbody fusion in the treatment of recurrent lumbar disc herniation.

Am J Transl Res 2022 15;14(4):2383-2392. Epub 2022 Apr 15.

Department of Spine Surgery, Honghui Hospital, Xi'an Jiaotong University Xi'an, China.

Objective: To explore the clinical effect of unilateral biportal endoscopic-assisted transforaminal lumbar interbody fusion (UBE-TLIF) in the treatment of recurrent lumbar disc herniation (RLDH).

Methods: The clinical data of 44 patients with RLDH treated by UBE-TLIF in our hospital from August 2020 to December 2020 were analysed retrospectively. The study indicators included intraoperative blood loss, operation time, bed rest time, and hospital stay. The follow-up data included the visual analogue score (VAS) of low back pain, Japanese Orthopaedic Association score (JOA), Oswestry disability index (ODI) score, and the short form 36 health survey questionnaire (SF-36) score preoperatively and 1 week and 6 months postoperatively.

Results: The average operation time was 179.15 ± 42.06 minutes, the average intraoperative blood loss was 132.67 ± 41.92 ml, the average bed rest time was 1.51 ± 0.42 days, and the average hospital stay was 4.82 ± 1.13 days. The VAS score of low back pain after the operation was lower than that before the operation (all P<0.0001). The ODI score, JOA score, and SF-36 scores at postoperative follow-up were significantly different from those before the operation (P<0.05). The satisfaction rate was 86.4% at 7 days after the operation and 95.4% at 6 months after the operation. The proportion of significant clinical efficacy was 18.2% (postoperative day 7) and 63.6% (postoperative month 6).

Conclusions: UBE-TLIF has the advantages of a rapid recovery, less intraoperative blood loss, a short bed rest and hospital stay, and a good medium-term clinical effect. It is a safe, reliable minimally invasive technique for surgical treatment of RLDH.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091115PMC
April 2022

Error-Related Negativity-Based Robot-Assisted Stroke Rehabilitation System: Design and Proof-of-Concept.

Front Neurorobot 2022 25;16:837119. Epub 2022 Apr 25.

Department of Biomedical Engineering, College of Engineering, Shantou University, Shantou, China.

Conventional rehabilitation systems typically execute a fixed set of programs that most motor-impaired stroke patients undergo. In these systems, the brain, which is embodied in the body, is often left out. Including the brains of stroke patients in the control loop of a rehabilitation system can be worthwhile as the system can be tailored to each participant and, thus, be more effective. Here, we propose a novel brain-computer interface (BCI)-based robot-assisted stroke rehabilitation system (RASRS), which takes inputs from the patient's intrinsic feedback mechanism to adapt the assistance level of the RASRS. The proposed system will utilize the patients' consciousness about their performance decoded through their error-related negativity signals. As a proof-of-concept, we experimented on 12 healthy people in which we recorded their electroencephalogram (EEG) signals while performing a standard rehabilitation exercise. We set the performance requirements beforehand and observed participants' neural responses when they failed/met the set requirements and found a statistically significant ( < 0.05) difference in their neural responses in the two conditions. The feasibility of the proposed BCI-based RASRS was demonstrated through a use-case description with a timing diagram and meeting the crucial requirements for developing the proposed rehabilitation system. The use of a patient's intrinsic feedback mechanism will have significant implications for the development of human-in-the-loop stroke rehabilitation systems.
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http://dx.doi.org/10.3389/fnbot.2022.837119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085417PMC
April 2022

Downregulation of MTAP promotes Tumor Growth and Metastasis by regulating ODC Activity in Breast Cancer.

Int J Biol Sci 2022 24;18(7):3034-3047. Epub 2022 Apr 24.

Guangdong Provincial Key Laboratory of Regional Immunity and Diseases, Department of Pharmacology and International Cancer Center, Shenzhen University Health Science Center, Shenzhen 518060, Guangdong, China.

5'-Methylthioadenosine phosphorylase (MTAP) is a key enzyme in the methionine salvage pathway and has been reported to suppress tumorigenesis. The MTAP gene is located at 9p21, a chromosome region often deleted in breast cancer (BC). However, the clinical and biological significance of MTAP in BC is still unclear. Here, we reported that MTAP was frequently downregulated in 41% (35/85) of primary BCs and 89% (8/9) of BC cell lines. Low expression of MTAP was significantly correlated with a poor survival of BC patients (P=0.0334). Functional studies showed that MTAP was able to suppress both and tumorigenic ability of BC cells, including migration, invasion, angiogenesis, tumor growth and metastasis in nude mice with orthotopic xenograft tumor of BC. Mechanistically, we found that downregulation of MTAP could increase the polyamine levels by activating ornithine decarboxylase (ODC). By treating the MTAP-repressing BC cells with specific ODC inhibitor Difluoromethylornithine (DFMO) or treating the MTAP-overexpressing BC cells with additional putrescine, metastasis-promoting or -suppressing phenotype of these MTAP-manipulated cells was significantly reversed, respectively. Taken together, our data suggested that MTAP has a critical metastasis-suppressive role by tightly regulating ODC activity in BC cells, which may serve as a prominent novel therapeutic target for advanced breast cancer treatment.
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http://dx.doi.org/10.7150/ijbs.67149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066107PMC
April 2022

Learning-based Intrinsic Reflectional Symmetry Detection.

IEEE Trans Vis Comput Graph 2022 May 6;PP. Epub 2022 May 6.

Reflectional symmetry is a ubiquitous pattern in nature. Previous works usually solve this problem by voting or sampling, suffering from high computational cost and randomness. In this paper, we propose a learning-based approach to intrinsic reflectional symmetry detection. Instead of directly finding symmetric point pairs, we parametrize this self-isometry using a functional map matrix, which can be easily computed given the signs of Laplacian eigenfunctions under the symmetric mapping. Therefore, we manually label the eigenfunction signs for a variety of shapes and train a novel neural network to predict the sign of each eigenfunction under symmetry. Our network aims at learning the global property of functions and consequently converts the problem defined on the manifold to the functional domain. By disentangling the prediction of the matrix into separated bases, our method generalizes well to new shapes and is invariant under perturbation of eigenfunctions. Through extensive experiments, we demonstrate the robustness of our method in challenging cases, including different topology and incomplete shapes with holes. By avoiding random sampling, our learning-based algorithm is over 20 times faster than state-of-the-art methods, and meanwhile, is more robust, achieving higher correspondence accuracy in commonly used metrics.
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http://dx.doi.org/10.1109/TVCG.2022.3172361DOI Listing
May 2022

A time-incorporated SOFA score-based machine learning model for predicting mortality in critically ill patients: A multicenter, real-world study.

Int J Med Inform 2022 07 21;163:104776. Epub 2022 Apr 21.

Department of Critical Care Medicine, Affiliated Jinling Hospital, School of Medicine, Southeast University& Nanjing University, Nanjing 210002, PR China; National Institute of Healthcare Data Science at Nanjing University, Nanjing, 210023, PR China.

Background: Organ dysfunction (OD) assessment is essential in intensive care units (ICUs). However, current OD assessment scores merely describe the number and the severity of each OD, without evaluating the duration of organ injury. The objective of this study is to develop and validate a machine learning model based on the Sequential Organ Failure Assessment (SOFA) score for the prediction of mortality in critically ill patients.

Material And Methods: Data from the eICU Collaborative Research Database and Medical Information Mart for Intensive Care (MIMIC) -III were mixed for model development. The MIMIC-IV and Nanjing Jinling Hospital Surgical ICU database were used as external test set A and set B, respectively. The outcome of interest was in-ICU mortality. A modified SOFA model incorporating time-dimension (T-SOFA) was stepwise developed to predict ICU mortality using extreme gradient boosting (XGBoost), support vector machine, random forest and logistic regression algorithms. Time-dimensional features were calculated based on six consecutive SOFA scores collected every 12 h within the first three days of admission. The predictive performance was assessed with the area under the receiver operating characteristic curves (AUROC) and calibration plot.

Results: A total of 82,132 patients from the real-world datasets were included in this study, and 7,494 patients (9.12%) died during their ICU stay. The T-SOFA M3 that incorporated the time-dimension features and age, using the XGBoost algorithm, significantly outperformed the original SOFA score in the validation set (AUROC 0.800 95% CI [0.787-0.813] vs. 0.693 95% CI [0.678-0.709], p < 0.01). Good discrimination and calibration were maintained in the test set A and B, with AUROC of 0.803, 95% CI [0.791-0.815] and 0.830, 95% CI [0.789-0.870], respectively.

Conclusions: The time-incorporated T-SOFA model could significantly improve the prediction performance of the original SOFA score and is of potential for identifying high-risk patients in future clinical application.
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http://dx.doi.org/10.1016/j.ijmedinf.2022.104776DOI Listing
July 2022

A randomized, controlled trial of intense pulsed light in combination with minocycline hydrochloride for the treatment of inflammatory acne vulgaris.

J Cosmet Dermatol 2022 Aug 16;21(8):3386-3392. Epub 2022 May 16.

Department of Dermatology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.

Background: It has been a long-term debate over the concomitant treatment of inflammatory acne vulgaris using intense pulsed light (IPL) and minocycline due to the photosensitivity of minocycline.

Objective: We aimed to evaluate the safety and efficiency of IPL combined with minocycline in treating acne vulgaris in a randomized trial.

Methods: A total of 40 patients were enrolled and randomly assigned into two groups which were either given minocycline (100 mg per day) for 8 weeks with IPL treatments three times at weeks 0, 4, and 8, or the same dosage of minocycline only. The evaluations for inflammatory lesion count, Investigator Global Assessment of Acne (IGA), erythema, and purpura indexes were taken before treatment and at weeks 4, 8, and 16.

Results: There were significant improvements in inflammatory lesion count, IGA scores, and purpura index in both groups as compared with the baseline at week 16 (p < 0.02). The concomitant therapy, but not minocycline only, significantly improved the erythema index (p = 0.40) at the 16 week as compared with the baseline. The group with combined treatment showed significantly continuous improvements in inflammatory lesion counts (p < 0.04) and IGA scores (p ≤ 0.02) at weeks 4, 8, and 16 as compared with the group given minocycline only. No severe adverse effects were observed during the trial.

Conclusion: IPL in combination with minocycline shows a better clinical efficacy for the treatment of inflammatory acne vulgaris than minocycline alone, and it is safe.
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http://dx.doi.org/10.1111/jocd.15046DOI Listing
August 2022

RUVBL1 promotes enzalutamide resistance of prostate tumors through the PLXNA1-CRAF-MAPK pathway.

Oncogene 2022 06 4;41(23):3239-3250. Epub 2022 May 4.

The Key Laboratory of Experimental Teratology, Ministry of Education and Department of Pathology, School of Basic Medical Sciences, Shandong University, Jinan, Shandong, 250012, China.

Although enzalutamide improves the overall survival of patients with metastatic prostate cancers, enzalutamide resistance (ENZR) will be inevitably developed. Emerging evidence support that alternative oncogenic pathways may bypass the androgen receptor (AR) signaling to promote ENZR progression, however, the underpinning mechanisms remain poorly defined. Here, we report that the expression of RuvB like AAA ATPase 1 (RUVBL1) is upregulated in ENZR cells and xenograft models and prostate tumors in patients. Enzalutamide increases RUVBL1 accumulation in the cytoplasm, which in turn enhances the recruitment of CRAF proto-oncogene serine/threonine kinase protein to plexin A1 (PLXNA1) and the subsequent activation of the downstream MAPK pathway. Co-overexpression of RUVBL1 and PLXNA1 defines a subgroup of prostate cancer (PCa) patients with a poor prognosis. Furthermore, pharmacological inhibition of RUVBL1 by CB-6644 suppresses ENZR cell proliferation and xenograft growth and allows re-sensitization of ENZR cells and xenografts to enzalutamide, indicating that RUVBL1 may act to substitute the AR signaling to promote cancer cell survival and ENZR development. Together, these findings may lead to the identification of RUVBL1 as a potential therapeutic target for ENZR tumors.
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http://dx.doi.org/10.1038/s41388-022-02332-8DOI Listing
June 2022

The clinical outcome from early versus delayed minimally invasive intervention for infected pancreatic necrosis: a systematic review and meta-analysis.

J Gastroenterol 2022 06 29;57(6):397-406. Epub 2022 Apr 29.

Center of Severe Acute Pancreatitis (CSAP), Department of Critical Care Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China.

Background: While the management of infected pancreatic necrosis (IPN) has evolved in the last two decades with the adoption of minimally invasive interventions (drainage ± debridement), it is unknown whether the principle of delaying intervention inherited from the open surgery era still applies. The aim of the current study was to investigate the impact of the timing of minimally invasive intervention on the outcomes of patients with IPN requiring intervention.

Methods: PubMed, Embase, MEDLINE and Web of Science databases were searched for appropriate studies. The primary outcome of interest was hospital mortality, the secondary outcomes were the incidence of complications during the hospitalization, including new-onset organ failure, gastrointestinal fistula or perforation, bleeding and length of hospital or intensive care unit (ICU) stay.

Results: Seven clinical studies were included with a total of 742 patients with IPN requiring intervention, of whom 321 received early intervention and 421 delayed intervention. Results from the meta-analysis showed that early minimally invasive intervention did not increase hospital mortality (odds ratio 1.65, 95% confidence interval 0.97-2.81; p = 0.06) but was associated with a remarkably prolonged hospital stay and an increased incidence of gastrointestinal fistula or perforation when compared with delayed intervention.

Conclusions: Although no firm conclusion can be drawn because of the quality of available studies, it does appear that timing of intervention is a risk factor for adverse outcomes and ought to be investigated more rigorously in prospective studies.
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http://dx.doi.org/10.1007/s00535-022-01876-6DOI Listing
June 2022

Motif-GCNs with Local and Non-Local Temporal Blocks for Skeleton-Based Action Recognition.

IEEE Trans Pattern Anal Mach Intell 2022 Apr 26;PP. Epub 2022 Apr 26.

Recent works have achieved remarkable performance for action recognition with human skeletal data by utilizing graph convolutional models. Existing models mainly focus on developing graph convolutions to encode structural properties of the skeletal graph. Some recent works further take sample-dependent relationships among joints into consideration. However, the complex relationships are difficult to learn. In this paper, we propose a motif-based graph convolution method, which makes use of sample-dependent latent relations among non-physically connected joints to impose a high-order locality and assigns different semantic roles to physical neighbors of a joint to encode hierarchical structures. Furthermore, we propose a sparsity-promoting loss function to learn a sparse motif adjacency matrix for latent dependencies in non-physical connections. For extracting effective temporal information, we propose an efficient local temporal block. It adopts partial dense connections to reuse temporal features in local time windows, and enrich a variety of information flow by gradient combination. In addition, we introduce a non-local temporal block to capture global dependencies among frames. Comprehensive experiments on four large-scale datasets show that our model outperforms the state-of-the-art methods. Our code is publicly available at https://github.com/wenyh1616/SAMotif-GCN.
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http://dx.doi.org/10.1109/TPAMI.2022.3170511DOI Listing
April 2022

Bilateral Superior Cervical Sympathectomy Activates Signal Transducer and Activator of Transcription 3 Signal to Alleviate Myocardial Ischemia-Reperfusion Injury.

Front Cardiovasc Med 2022 1;9:807298. Epub 2022 Apr 1.

Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Background: There is growing evidence about the effect of bilateral superior cervical sympathectomy on myocardial ischemia-reperfusion (I/R) injury. Studies have increasingly found that the signal transducer and activator of transcription 3 (STAT3) plays a protective role in myocardial I/R injury. However, the precise mechanism is unknown. The present study explored the bilateral superior cervical sympathectomy's effect and potential mechanism in mice myocardial I/R injury.

Methods: The left heart I/R injury model was created by ligating the anterior descending branch of the coronary artery for 30 min followed by reperfusion. Bilateral superior cervical sympathectomy was performed before myocardial I/R injury. To evaluate the effect of bilateral superior cervical sympathectomy on the myocardium, we examined the myocardial infarct size and cardiac function. Then, myocardial apoptosis, inflammation, and oxidative stress were detected on the myocardium. Furthermore, the expression of STAT3 signal in myocardial tissue was measured by western blotting. To further examine the cardioprotective effect of STAT3 after bilateral superior cervical sympathectomy, the STAT3 inhibitor (static) was utilized to inhibit the phosphorylation of STAT3.

Results: The results showed that the myocardial I/R injury decreased and the cardiac function recovered in the myocardial I/R injury after cervical sympathectomy. Meanwhile, cervical sympathectomy reduced the myocardial distribution of the sympathetic marker tyrosine hydroxylase (TH) and systemic sympathetic tone. And levels of oxidative stress, inflammatory markers, and apoptosis were reduced in myocardial tissue. We also found that the STAT3 signal was activated in myocardial tissue after cervical sympathectomy. STAT3 inhibitor can partially reverse the myocardial protective effect of cervical sympathectomy.

Conclusion: Bilateral superior cervical sympathectomy significantly alleviated myocardial I/R injury in mice. And activation of the STAT3 signal may play an essential role in this.
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http://dx.doi.org/10.3389/fcvm.2022.807298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010611PMC
April 2022
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