Publications by authors named "Lin Chen"

4,277 Publications

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Chrysin Induced Cell Apoptosis Through /let-7a/ Axis in Gastric Cancer Cells and Inhibited Tumor Growth.

Front Oncol 2021 3;11:651644. Epub 2021 Jun 3.

Laboratory Animal Center, College of Animal Science, Jilin University, Changchun, China.

Background: Chrysin is a natural flavone that is present in honey and has exhibited anti-tumor properties. It has been widely studied as a therapeutic agent for the treatment of various types of cancers. The objectives of this present study were to elucidate how chrysin regulates non-coding RNA expression to exert anti-tumor effects in gastric cancer cells.

Methods: Through the use of RNA sequencing, we investigated the differential expression of mRNAs in gastric cancer cells treated with chrysin. Furthermore, and let-7a overexpression and knockdown were conducted. Other features, including cell growth, apoptosis, migration and invasion, were also analyzed. Knockout of the gene was generated using the CRISPR/Cas9 system for tumor growth analysis .

Results: Our results identified as a differentially expressed mRNA that is down-regulated following treatment with chrysin. Moreover, the results showed that chrysin can induce cellular apoptosis and inhibit cell migration and invasion. To further determine the underlying mechanism of COPB2 expression, we investigated the expression of the long non-coding RNA (lncRNA) and microRNA let-7a. Our results showed that treatment with chrysin significantly increased let-7a expression and reduced the expression of and . In addition, our results demonstrated that reduced expression of markedly promotes cell apoptosis. Finally, data suggested that expression is related to tumor growth.

Conclusions: This study suggests that chrysin exhibited anti-tumor effects through a /let-7a/ axis.
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http://dx.doi.org/10.3389/fonc.2021.651644DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209501PMC
June 2021

Exosomes: roles and therapeutic potential in osteoarthritis.

Bone Res 2020 Jun 19;8(1):25. Epub 2020 Jun 19.

Department of Wound Repair and Rehabilitation Medicine, Center of Bone Metabolism and Repair, Laboratory for Prevention and Rehabilitation of Training Injuries, State Key Laboratory of Trauma, Burns and Combined Injury, Trauma Center, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, China.

Exosomes participate in many physiological and pathological processes by regulating cell-cell communication, which are involved in numerous diseases, including osteoarthritis (OA). Exosomes are detectable in the human articular cavity and were observed to change with OA progression. Several joint cells, including chondrocytes, synovial fibroblasts, osteoblasts, and tenocytes, can produce and secrete exosomes that influence the biological effects of targeted cells. In addition, exosomes from stem cells can protect the OA joint from damage by promoting cartilage repair, inhibiting synovitis, and mediating subchondral bone remodeling. This review summarizes the roles and therapeutic potential of exosomes in OA and discusses the perspectives and challenges related to exosome-based treatment for OA patients in the future.
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http://dx.doi.org/10.1038/s41413-020-0100-9DOI Listing
June 2020

Traffic stops do not prevent traffic deaths.

J Trauma Acute Care Surg 2021 Jul;91(1):141-147

From the Research in Surgical Outcomes and Effectiveness, Department of Surgery (A.L.S., L.C., K.C.B., P.A.L., M.L.M., C.W.T.), University Hospitals Cleveland Medical Center; Case Western Reserve University School of Medicine (V.P.H., L.C., K.C.B., P.A.L., A.M.L., M.L.M., C.W.T.); Division of Trauma, Critical Care, Burns, & Acute Care Surgery, Department of Surgery (V.P.H., A.M.L.), MetroHealth Medical Center, Cleveland, Ohio.

Objectives: Amid growing calls for police reform, it is imperative to reassess whether police actions designed to improve public safety are associated with injury prevention. This study aims to examine the relationship between the police traffic stops (PTSs) and motor vehicle crash (MVC) deaths at the state level. We hypothesize that increased PTSs would be associated with reduced MVC deaths.

Methods: We retrospectively analyzed PTSs and MVC deaths at the state level from 2004 to 2016. Police traffic stops data were from 33 state patrols from the Stanford Open Policing Project. The MVC deaths data were collected from the National Highway Traffic Safety Administration. The vehicle miles traveled data were from the Federal Highway Administration Office of Highway Policy Information. All data were adjusted per 100 million vehicle miles traveled (100MVMT) and were analyzed as state-level time series cross-sectional data. The dependent variable was MVC deaths per 100MVMT, and the independent variable was number of PTSs per 100MVMT. We performed panel data analysis accounting for random and fixed state effects and changes over time.

Results: Thirty-three state patrols with 235 combined years were analyzed, with a total of 161,153,248 PTSs. The PTS rate varied by state and year. Nebraska had the highest PTS rate (3,637/100MVMT in 2004), while Arizona had the lowest (0.17/100MVMT in 2009). Motor vehicle crash deaths varied by state and year, with the highest death rate occurring in South Carolina in 2005 (2.2/100MVMT) and the lowest in Rhode Island in 2015 (0.57/100MVMT). After accounting for year and state-level variability, no association was found between PTS and the MVC death rates.

Conclusion: State patrol traffic stops are not associated with reduced MVC deaths. Strategies to reduce death from MVC should consider alternative strategies, such as motor vehicle modifications, community-based safety initiatives, improved access to health care, or prioritizing trauma system.

Level Of Evidence: Retrospective epidemiological study, level IV.
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http://dx.doi.org/10.1097/TA.0000000000003163DOI Listing
July 2021

Silencing of the Long Non-Coding RNA TTN-AS1 Attenuates the Malignant Progression of Osteosarcoma Cells by Regulating the miR-16-1-3p/TFAP4 Axis.

Front Oncol 2021 1;11:652835. Epub 2021 Jun 1.

Department of Spine Surgery, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Objectives: Osteosarcoma (OS) is a type of bone malignancy. This study attempted to explore the effect of long non-coding RNA TTN-AS1 (TTN-AS1) on OS and to determine its molecular mechanisms.

Methods: The expression of TTN-AS1, microRNA-16-1-3p (miR-16-1-3p), and transcription factor activating enhancer binding protein 4 (TFAP4) in OS was assessed using qRT-PCR. The OS cell proliferation, migration, and invasion were measured using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), wound-healing, and transwell assays. N-cadherin and MMP-2 protein level was determined with western blot. Interactions between TTN-AS1 and miR-16-1-3p or TFAP4 and miR-16-1-3p were confirmed using the dual-luciferase reporter assay. Additionally, an OS xenograft tumor model was constructed to assess the effect of TTN-AS1 on tumor growth.

Results: TTN-AS1 and TFAP4 expression was increased in OS, while miR-16-1-3p expression was decreased. TTN-AS1 silencing restrained OS cell proliferation, migration, invasion, N-cadherin and MMP-2 protein expression, and hindered tumor growth. MiR-16-1-3p overexpression retarded the malignant behavior of OS cells. TTN-AS1 played a carcinostatic role by down-regulating miR-16-1-3p in the OS cells. Moreover, miR-16-1-3p inhibition or TFAP4 elevation weakened the suppressive effect of TTN-AS1 silencing on OS cell tumor progression.

Conclusion: TTN-AS1 promoted the proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of OS cells mediating the miR-16-1-3p/TFAP4 axis. TTN-AS1 may be a critical target for improving OS.
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http://dx.doi.org/10.3389/fonc.2021.652835DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204018PMC
June 2021

Additive quality influences the reservoir of antibiotic resistance genes during chicken manure composting.

Ecotoxicol Environ Saf 2021 Sep 15;220:112413. Epub 2021 Jun 15.

College of Resource and Environmental Sciences, Jiujiang University, Jiujiang 332005, China.

Aerobic composting is commonly used to dispose livestock manure and is an efficient way to reduce antibiotic resistance genes (ARGs). Here, the effects of different quality substrates on the fate of ARGs were assessed during manure composting. Results showed that the total relative abundances of ARGs and intI1 in additive treatments were lower than that in control, and high quality treatment with low C/N ratio and lignin significantly decreased the relative abundance of tetW, ermB, ermC, sul1 and sul2 at the end of composting. Additionally, higher quality treatment reduced the relative abundances of some pathogens such as Actinomadura and Pusillimonas, and some thermotolerant degrading-related bacteria comprising Pseudogracilibacillus and Sinibacillus on day 42, probably owing to the change of composting properties in piles. Structural equation models (SEMs) further verified that the physiochemical properties of composting were the dominant contributor to the variations in ARGs and they could also indirectly impact ARGs by influencing bacterial community and the abundance of intI1. Overall, these findings indicated that additives with high quality reduced the reservoir of antibiotic resistance genes of livestock manure compost.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112413DOI Listing
September 2021

Diversity and Inclusion in the Virtual Era.

Ann Thorac Surg 2021 Jun 14. Epub 2021 Jun 14.

Section of Thoracic Surgery, Department of Thoracic and Cardiovascular Surgery, Heart Vascular and Thoracic Institute, Cleveland Clinic.

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http://dx.doi.org/10.1016/j.athoracsur.2021.05.061DOI Listing
June 2021

Status quo and future prospects of artificial neural network from the perspective of gastroenterologists.

World J Gastroenterol 2021 Jun;27(21):2681-2709

Department of General Surgery & Institute of General Surgery, Chinese People's Liberation Army General Hospital, Beijing 100853, China.

Artificial neural networks (ANNs) are one of the primary types of artificial intelligence and have been rapidly developed and used in many fields. In recent years, there has been a sharp increase in research concerning ANNs in gastrointestinal (GI) diseases. This state-of-the-art technique exhibits excellent performance in diagnosis, prognostic prediction, and treatment. Competitions between ANNs and GI experts suggest that efficiency and accuracy might be compatible in virtue of technique advancements. However, the shortcomings of ANNs are not negligible and may induce alterations in many aspects of medical practice. In this review, we introduce basic knowledge about ANNs and summarize the current achievements of ANNs in GI diseases from the perspective of gastroenterologists. Existing limitations and future directions are also proposed to optimize ANN's clinical potential. In consideration of barriers to interdisciplinary knowledge, sophisticated concepts are discussed using plain words and metaphors to make this review more easily understood by medical practitioners and the general public.
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http://dx.doi.org/10.3748/wjg.v27.i21.2681DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173384PMC
June 2021

Association of Differential Leukocyte Count With Incident Abdominal Aortic Aneurysm Over 22.5 Years: The ARIC Study.

Arterioscler Thromb Vasc Biol 2021 Jun 17:ATVBAHA121315903. Epub 2021 Jun 17.

Division of Epidemiology and Community Health, School of Public Health, University of Minnesota Medical School, Minneapolis. (R.R.P., A.R.F., K.P., P.L.L., R.T.D., J.S.P., W.T.).

Objective: Leukocytes contribute to the development of abdominal aortic aneurysm (AAA). We evaluated whether associations of differential leukocyte counts with AAA persist after accounting for traditional risk factors of AAA. Approach and Results: Among 11 217 adults from the Atherosclerosis Risk in Communities Study, we evaluated associations of differential leukocyte counts at baseline (1987-1989) with incident AAAs over a median follow-up of 22.5 years, using Cox proportional hazards regression. Each differential leukocyte count was categorized into 5 groups-below normal, tertiles within the normal range, and above normal, with the first tertile serving as the referent. We identified 377 incident AAAs through 2011, using hospital discharge diagnoses, linked Medicare records, or death certificates. At baseline, higher neutrophil, monocyte, and eosinophil counts were associated with higher risk of AAA, independent of smoking, other differential leukocyte counts, and other traditional risk factors. The association with incident AAA was the strongest for above normal neutrophil count, with an adjusted hazard ratio (95% CI) of 2.17 (1.29-3.64). Below normal neutrophil, lymphocyte, eosinophil and basophil counts were associated with higher risk of AAA with adjusted hazard ratio (95% CI) between 1.86 (1.04-3.35) and 1.62 (1.10-2.39).

Conclusions: Higher neutrophil, monocyte, and eosinophil counts in midlife are associated with higher risk of AAA, even after accounting for traditional risk factors such as smoking, obesity, and atherosclerosis. This suggests the need to identify nontraditional risk factors and treatment strategies to mitigate the residual risk of AAA conferred by midlife inflammation. Whether immunosuppression is associated with higher risk of AAA needs further investigation.
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http://dx.doi.org/10.1161/ATVBAHA.121.315903DOI Listing
June 2021

Event-related Beta EEG changes Induced by Various Neuromuscular Electrical Stimulation: A Pilot Study.

IEEE Trans Neural Syst Rehabil Eng 2021 Jun 15;PP. Epub 2021 Jun 15.

Previous results demonstrated that neuromuscular electrical stimulation (NMES) with various configurations could induce different activity at both the central and peripheral levels. Although NMES generating different peripheral movements have been studied, it is still unclear whether the difference in NMES-induced cortical activity is due to movement-or stimulation-related differences. Because NMES-induced cortical activity impacts motor function recovery, it is essential to know when NMES with various configurations evoke the same movement, whether the induced cortical activity is still different. Four NMES configurations: 1) Eight-let Frequency Trains, 2) Doublet frequency trains (DFT), 3) Constant-frequency trains with narrow-pulse, and 4) wide-pulse, were delivered to the right biceps brachii muscle in nine healthy young adults. We adjusted the intensities of these NMES to evoke the same elbow flexion and compared the cortical activities over sensorimotor regions. Our results showed that the four NMES patterns induced different beta-band Event-Related Desynchronization (ERD), with the DFT providing the strongest ERD value given the same NMES-induced elbow flexion (p<0.05). This difference is possibly due to NMES with different configuration activated in the amount of afferent proprioceptive fibers. Our pilot study suggests that the NMES-induced beta-band ERD may be an additional factor to consider when selecting the NMES configuration for a better motor function recovery.
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http://dx.doi.org/10.1109/TNSRE.2021.3089478DOI Listing
June 2021

Managing Atrial Fibrillation in Patients With Heart Failure and Reduced Ejection Fraction: A Scientific Statement From the American Heart Association.

Circ Arrhythm Electrophysiol 2021 Jun 15;14(6):HAE0000000000000078. Epub 2021 Jun 15.

Atrial fibrillation and heart failure with reduced ejection fraction are increasing in prevalence worldwide. Atrial fibrillation can precipitate and can be a consequence of heart failure with reduced ejection fraction and cardiomyopathy. Atrial fibrillation and heart failure, when present together, are associated with worse outcomes. Together, these 2 conditions increase the risk of stroke, requiring oral anticoagulation in many or left atrial appendage closure in some. Medical management for rate and rhythm control of atrial fibrillation in heart failure remain hampered by variable success, intolerance, and adverse effects. In multiple randomized clinical trials in recent years, catheter ablation for atrial fibrillation in patients with heart failure and reduced ejection fraction has shown superiority in improving survival, quality of life, and ventricular function and reducing heart failure hospitalizations compared with antiarrhythmic drugs and rate control therapies. This has resulted in a paradigm shift in management toward nonpharmacological rhythm control of atrial fibrillation in heart failure with reduced ejection fraction. The primary objective of this American Heart Association scientific statement is to review the available evidence on the epidemiology and pathophysiology of atrial fibrillation in relation to heart failure and to provide guidance on the latest advances in pharmacological and nonpharmacological management of atrial fibrillation in patients with heart failure and reduced ejection fraction. The writing committee's consensus on the implications for clinical practice, gaps in knowledge, and directions for future research are highlighted.
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http://dx.doi.org/10.1161/HAE.0000000000000078DOI Listing
June 2021

End-Group Modifications with Bromine and Methyl in Nonfullerene Acceptors: The Effect of Isomerism.

ACS Appl Mater Interfaces 2021 Jun 15. Epub 2021 Jun 15.

Shenzhen Grubbs Institute and Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055, China.

The development of isomeric molecules has been widely exploited in molecular structures associated with organic solar cells (OSC) and is an effective pathway to finely tune the photoelectric properties and device performance. The molecular properties of nonfullerene acceptors and the morphology of blend films can be effectively controlled by manipulating isomeric substituent positions on benzene-fused end-capping groups (EG) in acceptors. Here, three isomeric EGs were designed and synthesized which simultaneously possess an electron-withdrawing bromine and an electron-donating methyl substituent. By linking three isomeric EGs, , , and each with the BTP-CHO core, three isomeric small-molecule acceptors (SMA) were obtained. The power conversion efficiency (PCE) of PM6:-based OSCs is 13.43%, is much higher than that of PM6:- (11.92%) and PM6:- (11.08%) based devices. Our results show that isomeric EGs can provide strategies to tune the absorption spectra of SMAs, intramolecular charge transfer (ICT) and electron mobility of organic semiconductor device, and ultimately increase the performance of nonfullerene acceptors.
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http://dx.doi.org/10.1021/acsami.1c08060DOI Listing
June 2021

Systematic analysis of safety profile for darunavir and its boosted agents using data mining in the FDA Adverse Event Reporting System database.

Sci Rep 2021 Jun 14;11(1):12438. Epub 2021 Jun 14.

Department of Pharmacy, Chongqing Health Center for Women and Children, Chongqing, 400021, China.

This current investigation was aimed to generate signals for adverse events (AEs) of darunavir-containing agents by data mining using the US Food and Drug Administration Adverse Event Reporting System (FAERS). All AE reports for darunavir, darunavir/ritonavir, or darunavir/cobicistat between July 2006 and December 2019 were identified. The reporting Odds Ratio (ROR), proportional reporting ratio (PRR), and Bayesian confidence propagation neural network (BCPNN) were used to detect the risk signals. A suspicious signal was generated only if the results of the three algorithms were all positive. A total of 10,756 reports were identified commonly observed in hepatobiliary, endocrine, cardiovascular, musculoskeletal, gastrointestinal, metabolic, and nutrition system. 40 suspicious signals were generated, and therein 20 signals were not included in the label. Severe high signals (i.e. progressive extraocular muscle paralysis, acute pancreatitis, exfoliative dermatitis, acquired lipodystrophy and mitochondrial toxicity) were identified. In pregnant women, umbilical cord abnormality, fetal growth restriction, low birth weight, stillbirth, premature rupture of membranes, premature birth and spontaneous abortion showed positive signals. Darunavir and its boosted agents induced AEs in various organs/tissues, and were shown to be possibly associated with multiple adverse pregnant conditions. This study highlighted some novel and severe AEs of darunavir which need to be monitored prospectively.
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http://dx.doi.org/10.1038/s41598-021-91549-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203613PMC
June 2021

Could neoadjuvant chemotherapy increase postoperative complication risk of laparoscopic total gastrectomy? A mono-institutional propensity score-matched study in China.

World J Gastrointest Surg 2021 May;13(5):429-442

Department of General Surgery, Institute of General Surgery, Chinese PLA General Hospital, Beijing 100853, China.

Background: The potential survival benefit of neoadjuvant chemotherapy (NC) in patients with advanced gastric cancer has been widely recognized. With the development of minimally invasive surgery, which is represented by laparoscopy, the effect of NC on the safety of laparoscopic gastrectomy remains to be further explored.

Aim: To compare the short-term outcomes of laparoscopic total gastrectomy (LTG) after NC (NC-LTG) with LTG alone.

Methods: A total of 92 patients who underwent NC-LTG and 381 patients who received LTG alone at the Chinese PLA General Hospital between September 2015 and September 2020 were retrospectively included in our study. We used propensity-score matching (PSM) to balance baseline bias. After 1:1 PSM, 73 patients were included in each group with no statistically significant difference in baseline characteristics.

Results: The NC-LTG group exhibited a longer operation time (244.10 ± 48.13 min 225.74 ± 45.33 min, = 0.019) and increased intraoperative blood loss [150 (100-300) mL 100 (100-200) mL, = 0.011] compared to the LTG group. The 30-d postoperative morbidity of the NC-LTG group was 20.5% (15/73), and that of the LTG group was 13.7% (10/73). There were no significant differences in 30-d severe complication rates or anastomotic leakage rates. Subgroup analysis showed that the patients with pTNM (pathological tumor-node-metastasis classification) T0N0-II in the NC-LTG group underwent a longer operation than the LTG group, while no significant difference was found in any perioperative index for the pTNM III patients. A multivariate analysis showed that an operation time longer than 240 min was an independent risk factor (odds ratio = 3.021, 95% confidence interval: 1.160-7.868, = 0.024), while NC was not an independent risk factor for postoperative complications in LTG.

Conclusion: Despite a longer operation time and more blood loss after NC-LTG, which indicate surgical difficulty, NC-LTG exhibits acceptable short-term outcomes compared to LTG, suggesting the safety and feasibility of NC-LTG.
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http://dx.doi.org/10.4240/wjgs.v13.i5.429DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167844PMC
May 2021

Edge-enhancing gradient echo with multi-image co-registration and averaging (EDGE-MICRA) for targeting thalamic centromedian and parafascicular nuclei.

Neuroradiol J 2021 Jun 13:19714009211021781. Epub 2021 Jun 13.

Department of Neurosurgery, Mayo Clinic, Florida, USA.

Background And Purpose: Deep brain stimulation of the thalamus is an effective treatment for multiple neurological disorders. The centromedian and parafascicular nuclei are recently emerging targets for multiple conditions, such as epilepsy and Tourette syndrome; however, their limited visibility on conventional magnetic resonance imaging sequences has been a major obstacle. The goal of this study was to demonstrate the feasibility of a high-resolution and high-contrast targeting sequence for centromedian-parafascicular deep brain stimulation using a recently described magnetic resonance imaging sequence, three-dimensional edge-enhancing gradient echo.

Methods: The three-dimensional edge-enhancing gradient echo sequence was performed on a normal volunteer for a total of six acquisitions. Multi-image co-registration and averaging was performed by first co-registering each of the six scans and then averaging to produce an edge-enhancing gradient echo-multi-image co-registration and averaging scan. The averaging was also performed for two, three, four and five scans to assess the change in the signal-to-noise ratio and identify the ideal balance of image quality and scan time.

Results: The edge-enhancing gradient echo-multi-image co-registration and averaging scan allowed clear boundary delineation of the centromedian and parafascicular nuclei. The signal-to-noise ratio increased as a function of increasing scan number, but the added gain was small beyond four scans for the imaging parameters used in this study.

Conclusions: The recently described three-dimensional edge-enhancing gradient echo sequence provides an easily implementable approach, using widely available magnetic resonance imaging technology without complex post-processing techniques, to delineate centromedian and parafascicular nuclei for deep brain stimulation targeting.
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http://dx.doi.org/10.1177/19714009211021781DOI Listing
June 2021

The miRNA expression profile directly reflects the energy metabolic differences between slow and fast muscle with nutritional regulation of the Chinese perch (Siniperca chuatsi).

Comp Biochem Physiol A Mol Integr Physiol 2021 Jun 10;259:111003. Epub 2021 Jun 10.

Department of Bioengineering and Environmental Science, Changsha University, Changsha, Hunan 410003, China; Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, Department of Biological and Environmental Engineering, Changsha University, Changsha 410022, China. Electronic address:

Fish skeletal muscles are composed of two distinct types, slow and fast muscles, and they play important roles in maintaining the body's movement and energy metabolism. The two types of muscle are easy to separate, so they are often used as the model system for studies on their physiological and functional characteristics. In this study, we revealed that the carbohydrate and lipid metabolic KEGG pathways are different between slow and fast muscles of Chinese perch with transcriptome analysis. In fast muscle, glucose metabolism was catabolic with higher glycolysis capacity, while in slow muscle, glucose metabolism was anabolic with more glycogen synthesis. In addition, oxidative metabolism in slow muscle was stronger than that in fast muscle. By analyzing the expression levels of 40 miRNAs involved in metabolism in the muscles of Chinese perch, 18 miRNAs were significantly upregulated and 7 were significantly downregulated in slow muscle compared with fast muscle. Based on functional enrichment analysis of their target genes, the differential expression levels of 17 miRNAs in slow and fast muscles were reflected in their carbohydrate and lipid metabolism. Among these, 15 miRNAs were associated with carbohydrate metabolism, and 6 miRNAs were associated with lipid metabolism. After 3 days of starvation, the expression levels of 15 miRNAs involved in glucose metabolism in fast and slow muscles increased. However, after 7 days of starvation, the mRNA levels of miR-22a, miR-23a, miR-133a-3p, miR-139, miR-143, miR-144, miR-181a and miR-206 decreased to basal levels. Our data suggest that the possible reason for the difference in glucose and lipid metabolism is that more miRNAs inhibit the expression of target genes in slow muscle.
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http://dx.doi.org/10.1016/j.cbpa.2021.111003DOI Listing
June 2021

Resveratrol ameliorated endothelial injury of thoracic aorta in diabetic mice and Gly-LDL-induced HUVECs through inhibiting TLR4/HIF-1α.

J Cell Mol Med 2021 Jun 10. Epub 2021 Jun 10.

Department of Endocrinology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

To explore the effects of resveratrol on the levels of inflammatory cytokines and Toll-like receptor-4/ hypoxia-inducible transcription factors-1α (TLR4/HIF-1α) signalling pathway in diabetes mellitus. C57BL/6 mice received intraperitoneal injection of streptozocin for constructing diabetic mice models. Human umbilical vein endothelial cells (HUVECs) were treated with 50 µg/mL Gly-LDL for inducing injury models. 10, 100 and 1000 mmol/L resveratrol were obtained and added into each group. Haematoxylin-eosin (H&E) staining was used for histological evaluation. CCK8 assay was performed for determination of cell viability, and Transwell assay was implemented for detecting cell migration ability. Cell apoptosis was analysed using flow cytometry. The content of inflammatory factors including interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α), vascular adhesion molecule-1 (VCAM-1) and vascular endothelial growth factor (VEGF) were measured by ELISA. GST pull-down assay was employed for determining interactions between TLR4 and HIF-1α. The protein expression of TLR4 and HIF-1α was detected using Western blotting and immunohistochemistry, while relative mRNA expression was measured by RT-qRCR. Resveratrol could reduce bodyweight and ameliorate endothelial injury of thoracic aorta in diabetic mice. Both in vivo and in vitro results revealed that the level of IL-6, TNF-α, VCAM-1 and VEGF was significantly down-regulated after being treated with resveratrol. Resveratrol inhibited the increase of MDA and ROS and increased the level of SOD in diabetic mice. Western blotting, IHC and RT-qPCR results showed that the levels of TLR4 and HIF-1α were significantly down-regulated in resveratrol group. Overexpression of TLR4 or HIF-1α could reverse the effect of resveratrol. GST pull-down elucidated that there might be a close interaction between TLR4 and HIF-1α. Resveratrol ameliorated endothelial injury of thoracic aorta in diabetic mice and Gly-LDL-induced HUVECs through inhibiting TLR4/HIF-1α signalling pathway.
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http://dx.doi.org/10.1111/jcmm.16584DOI Listing
June 2021

Hypertonic Dextrose Stimulates Chondrogenic Cells to Deposit Collagen and Proliferate.

Cartilage 2021 Jun 10:19476035211014572. Epub 2021 Jun 10.

Department of Family and Community Medicine, Penn State College of Medicine, Hershey, PA, USA.

Objective: Hypertonic dextrose (HD) injections (prolotherapy) for osteoarthritis are reported to reduce pain. Cartilage regeneration is hypothesized as a mechanism. This study identifies an HD concentration that stimulates chondrogenic cells to increase metabolic activity and assesses whether this concentration affects collagen deposition and proliferation.

Design: ATDC5 chondrogenic cells were cultured in normoglycemic DMEM/F12 medium, treated with concentrations of HD (4-400 mM), and assessed with PrestoBlue. Advanced light microscopy was used to conduct live imaging of collagen deposition through second harmonic generation microscopy (SHG) and proliferation via 2-photon excitation microscopy. Proliferation was additionally assessed with hemocytometer counts.

Results: A linear regression model found that, relative to the 4 mM baseline control, cells treated with 200 mM had a higher mean absorbance ( = 0.023) and cells treated with 250 mM were trending toward a higher mean absorbance ( = 0.076). Polynomial regression interpolated 240 mM as producing the highest average absorbance. Hemocytometer counts validated 250 mM as stimulating proliferation compared with the 4 mM control ( < 0.01). A concentration of 250 mM HD led to an increase in collagen deposition compared with that observed in control ( < 0.05). This HD concentration also led to increases in proliferation of ATDC5 cells relative to that of control ( < 0.001).

Conclusions: A 250 mM HD solution appears to be associated with increased metabolic activity of chondrocytes, increased collagen deposition, and increased chondrocyte proliferation. These results support clinical prolotherapy research suggesting that intra-articular HD joint injections reduce knee pain. Further study of HD and cellular processes is warranted.
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http://dx.doi.org/10.1177/19476035211014572DOI Listing
June 2021

Exploring the molecular basis of UG-rich RNA recognition by the human splicing factor TDP-43 using molecular dynamics simulation and free energy calculation.

J Comput Chem 2021 Jun 9. Epub 2021 Jun 9.

College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar, China.

Transactivation response element RNA/DNA-binding protein 43 (TDP-43) is involved in the regulation of alternative splicing of human neurodegenerative disease-related genes through binding to long UG-rich RNA sequences. Mutations in TDP-43, most in the homeodomain, cause neurological disorders such as amyotrophic lateral sclerosis and fronto temporal lobar degeneration. Several mutants destabilize the structure and disrupt RNA-binding activity. The biological functions of these mutants have been characterized, but the structural basis behind the loss of RNA-binding activity is unclear. Focused on the specific TDP-43-ssRNA complex (PDB code 4BS2), we applied molecular dynamics simulations and the molecular mechanics Poisson-Boltzmann surface area free energy calculation to characterize and explore the structural and dynamic effects between ssRNA and TDP-43. The energetic analysis indicated that the intermolecular van der Waals interaction and nonpolar solvation energy play an important role in the binding process of TDP-43 and ssRNA. Compared with the wild-type TDP-43, the reduction of the polar or non-polar interaction between all the mutants F149A, D105A/S254A, R171A/D174A, F147L/F149L/F229L/F231L and ssRNA is the main reason for the reduction of its binding free energy. Decomposing energies suggested that the extensive interactions between TDP-43 and the nitrogenous bases of ssRNA are responsible for the specific ssRNA recognition by TDP-43. These results elucidated the TDP-43-ssRNA interaction comprehensively and further extended our understanding of the previous experimental data. The uncovering of TDP-43-ssRNA recognition mechanism will provide us useful insights and new chances for the development of anti-neurodegenerative drugs.
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http://dx.doi.org/10.1002/jcc.26704DOI Listing
June 2021

Dicer Deletion in Astrocytes Inhibits Oligodendroglial Differentiation and Myelination.

Neurosci Bull 2021 Jun 9. Epub 2021 Jun 9.

Department of Histology and Embryology, Chongqing Key Laboratory of Neurobiology, Brain and Intelligence Research Key Laboratory of Chongqing Education Commission, Third Military Medical University (Army Medical University), Chongqing, 400038, China.

Increasing evidence has shown that astrocytes are implicated in regulating oligodendrocyte myelination, but the underlying mechanisms remain largely unknown. To understand whether microRNAs in astrocytes function in regulating oligodendroglial differentiation and myelination in the developing and adult CNS, we generated inducible astrocyte-specific Dicer conditional knockout mice (hGFAP-CreERT; Dicer fl/fl). By using a reporter mouse line (mT/mG), we confirmed that hGFAP-CreERT drives an efficient and astrocyte-specific recombination in the developing CNS, upon tamoxifen treatment from postnatal day 3 (P3) to P7. The Dicer deletion in astrocytes resulted in inhibited oligodendroglial differentiation and myelination in the developing CNS of Dicer cKO mice at P10 and P14, and did not alter the densities of neurons or axons, indicating that Dicer in astrocytes is required for oligodendrocyte myelination. Consequently, the Dicer deletion in astrocytes at P3 resulted in impaired spatial memory and motor coordination at the age of 9 weeks. To understand whether Dicer in astrocytes is also required for remyelination, we induced Dicer deletion in 3-month-old mice and then injected lysolecithin into the corpus callosum to induce demyelination. The Dicer deletion in astrocytes blocked remyelination in the corpus callosum 14 days after induced demyelination. Together, our results indicate that Dicer in astrocytes is required for oligodendroglia myelination in both the developing and adult CNS.
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http://dx.doi.org/10.1007/s12264-021-00705-9DOI Listing
June 2021

Development and Validation of an Autophagy-Stroma-Based Microenvironment Gene Signature for Risk Stratification in Colorectal Cancer.

Onco Targets Ther 2021 31;14:3503-3515. Epub 2021 May 31.

Department of gastrointestinal surgery of the First Hospital of China Medical University, Shenyang, 110001, People's Republic of China.

Background: Colorectal cancer is the fourth most common cancer and the second leading cause of cancer-related death in the USA. The aim of this study was to establish a tumor gene signature based on tumor stromal cell and autophagy for predicting the risk of recurrence in patients with colorectal cancer.

Methods: We used "Rtsne" and "xCell" R packages to estimate autophagy and stroma status, respectively. The discovery cohort used microarray gene expression data retrieved from the GSE39582 dataset. The Cox regression model and Least Absolute Shrinkage and Selection Operator (LASSO) were used to identify prognostic genes and to construct an autophagy-stroma-based gene signature. Moreover, external validation was conducted using GSE17538, GSE38832, TCGA database, and patient data obtained from the First Hospital of China Medical University (CMU).

Results: The LASSO model identified three genes (, and ) which were used to develop a risk stratification gene signature. The autophagy-stroma-based gene signature was identified as an independent prognostic factor by multivariate analysis (p = 0.0023). The results were validated in GSE17538 (p=0.0062), GSE38832 (p=0.028), TCGA (p=0.046) database, and patient data obtained from the First Hospital of China Medical University (CMU) (p=0.027).

Conclusion: We have established and verified a feasible prognostic model of colorectal cancer based on autophagy and stromal cell characteristics of patients. The model can be used to evaluate recurrence risk of cancer patients, and the hub genes in the model provide potential targets for targeted colorectal cancer treatment.
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http://dx.doi.org/10.2147/OTT.S312003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180295PMC
May 2021

Probing penile hemodynamics by using photoplethysmography as objective indicators for male erection quality and sexual function.

Sci Rep 2021 Jun 8;11(1):12019. Epub 2021 Jun 8.

Department of Biomedical Sciences and Engineering, National Central University, Taoyuan, 320, Taiwan.

Erectile dysfunction (ED) is mostly due to the lack of blood flow into the penis. In the past 20 years, near-infrared spectroscopy (NIRS) was used in measuring the concentrations and temporal dynamics of different hemoglobin types. However, the dynamics of the light absorption (photoplethysmography; PPG) have not been applied to survey penile hemodynamics and erection quality. This paper compared the use of photoplethysmography (PPG) to study vascular ED with standard penile Doppler ultrasonography. Men diagnosed with vascular ED for at least 6 months and nominated for penile ultrasonography were included. PPG signals were collected during the ultrasound examination. All beat-to-beat PPG waveforms were aligned with the peak and averaged to one representative template waveform for feature analysis, including amplitude differences (APD) index, reflection time index (RTI), augmentation index (AI), and perfusion index (PI). An inverse correlation was found between end-erection amplitude and both erection hardness score (EHS) and resistive index (RI). APD index and EHS as well as the international index of erectile function-5 (IIEF) and RI were positively correlated. RTI and AI were inversely correlated to IIEF and RI. PI was positively correlated to RI. PPG may therefore be useful as a noninvasive, convenient, technique for sexual function evaluation.
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http://dx.doi.org/10.1038/s41598-021-91582-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187730PMC
June 2021

Enhancing myelin renewal reverses cognitive dysfunction in a murine model of Alzheimer's disease.

Neuron 2021 Jun 3. Epub 2021 Jun 3.

Brain and Intelligence Research Key Laboratory of Chongqing Education Commission, Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, China. Electronic address:

Severe cognitive decline is a hallmark of Alzheimer's disease (AD). In addition to gray matter loss, significant white matter pathology has been identified in AD patients. Here, we characterized the dynamics of myelin generation and loss in the APP/PS1 mouse model of AD. Unexpectedly, we observed a dramatic increase in the rate of new myelin formation in APP/PS1 mice, reminiscent of the robust oligodendroglial response to demyelination. Despite this increase, overall levels of myelination are decreased in the cortex and hippocampus of APP/PS1 mice and postmortem AD tissue. Genetically or pharmacologically enhancing myelin renewal, by oligodendroglial deletion of the muscarinic M1 receptor or systemic administration of the pro-myelinating drug clemastine, improved the performance of APP/PS1 mice in memory-related tasks and increased hippocampal sharp wave ripples. Taken together, these results demonstrate the potential of enhancing myelination as a therapeutic strategy to alleviate AD-related cognitive impairment.
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http://dx.doi.org/10.1016/j.neuron.2021.05.012DOI Listing
June 2021

An in-memory computing architecture based on two-dimensional semiconductors for multiply-accumulate operations.

Nat Commun 2021 06 7;12(1):3347. Epub 2021 Jun 7.

State Key Laboratory of ASIC and System, School of Microelectronics, Fudan University, Shanghai, China.

In-memory computing may enable multiply-accumulate (MAC) operations, which are the primary calculations used in artificial intelligence (AI). Performing MAC operations with high capacity in a small area with high energy efficiency remains a challenge. In this work, we propose a circuit architecture that integrates monolayer MoS transistors in a two-transistor-one-capacitor (2T-1C) configuration. In this structure, the memory portion is similar to a 1T-1C Dynamic Random Access Memory (DRAM) so that theoretically the cycling endurance and erase/write speed inherit the merits of DRAM. Besides, the ultralow leakage current of the MoS transistor enables the storage of multi-level voltages on the capacitor with a long retention time. The electrical characteristics of a single MoS transistor also allow analog computation by multiplying the drain voltage by the stored voltage on the capacitor. The sum-of-product is then obtained by converging the currents from multiple 2T-1C units. Based on our experiment results, a neural network is ex-situ trained for image recognition with 90.3% accuracy. In the future, such 2T-1C units can potentially be integrated into three-dimensional (3D) circuits with dense logic and memory layers for low power in-situ training of neural networks in hardware.
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http://dx.doi.org/10.1038/s41467-021-23719-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184885PMC
June 2021

Translation role of circRNAs in cancers.

J Clin Lab Anal 2021 Jun 7:e23866. Epub 2021 Jun 7.

Li Huili Hospital Affiliated to Ningbo University School, Ningbo, China.

Circular RNAs (circRNAs) constitute a class of covalently closed RNA molecules. With the continuous advancement of high-throughput sequencing technology and bioinformatics tools, many circRNAs have been identified in various human tissues and cell lines. Notably, recent studies have indicated that some circRNAs have translational functions. Internal ribosome entry sites and the N6-methyladenosine modification mediate cap-independent translation. This review describes these two translation mechanisms and verification methods at the molecular level. Databases (including ORF Finder, Pfam, BLASTp, CircRNADb, CircBase, CircPro, CircCode, IRESite, IRESbase) were used to analyze whether circRNAs have the structural characteristic of translation. CircRNA minigene reporter system containing green fluorescent protein (GFP) confirmed the translation potential of circRNAs. Also, we briefly summarize the roles of proteins/peptides encoded by circRNAs (circFBXW7, circFNDC3B, circLgr4, circPPP1R12A, circMAPK1, circβ-catenin, circGprc5a, circ-SHPRH, circPINTexon2, circAKT3) that have been verified thus far in human cancers (triple-negative breast cancer, colon cancer, gastric cancer, hepatocellular carcinoma, bladder cancer, glioblastoma). Those findings suggest circRNAs have a great implication in translation of the human genome.
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http://dx.doi.org/10.1002/jcla.23866DOI Listing
June 2021

Pharmacological Mechanisms Underlying the Therapeutic Effects of Danhong Injection on Cerebral Ischemia.

Evid Based Complement Alternat Med 2021 21;2021:5584809. Epub 2021 May 21.

Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China.

Background: Although Danhong injection (DHI) has been proved to be curative, the mechanism of its action against ischemia stroke (IS) is not clear. Here, we explored the therapeutic basis of DHI by network pharmacology.

Methods: Putative targets and activity scores for each compound in DHI were obtained from the Traditional Chinese Medicine System Pharmacology Database, Encyclopedia of Traditional Chinese Medicine, and Quantitative Structure Activity Relationships. Next, target proteins of IS were identified on GeneCards and CTD. Overlapping targets of DHI associated with IS were acquired via Venn diagram. GO and KEGG pathway analyses were done using WebGestalt. Cytoscape software was used for PPI network construction and hub nodes screening. Several validation studies were carried out by using AutoDock-Vina, label-free mass spectrometry, and transcriptome RNA-sequencing.

Results: The 37 active compounds and 66 targets were identified. Of these, 26 compounds and 41 targets belonged to diterpenoid quinones (DQs), which is the predominant category based on chemical structure. The results of enrichments analysis show that 8 DQs target proteins associated with IS were involved in several biological processes and signaling pathway such as apoptotic, cell cycle, cellular response to xenobiotic stimulus process, and the PI3K-Akt signaling. Moreover, 3 nodes in core module involved in PI3K-Akt signaling and 1 hub node were identified by PPI network analysis. Finally, the results of molecular docking and label-free mass spectrometry display good effect on hub node regulation in DHI treatment.

Conclusions: DQs is the predominant category of DHI and play an important role in antiapoptotic activity mediated by modulating PI3K-Akt signaling. Our findings offer insight into future research and clinical applications in IS therapy.
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http://dx.doi.org/10.1155/2021/5584809DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163534PMC
May 2021

Silencing Induces Facultative Parthenocarpy in Tomato.

Front Plant Sci 2021 20;12:672232. Epub 2021 May 20.

Key Laboratory of Horticultural Crop Biology and Germplasm Innovation in South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou, China.

Facultative parthenocarpy is of great practical value. However, the molecular mechanism underlying facultative parthenocarpy remains elusive. Transcriptional co-repressors (TPL) act as a central regulatory hub controlling all nine phytohormone pathways. Previously, we proved that SlTPLs participate in the auxin signaling pathway by interacting with auxin/indole acetic acid (Aux/IAAs) in tomato; however, their function in fruit development has not been studied. In addition to their high expression levels during flower development, the interaction between SlTPL1 and SlIAA9 stimulated the investigation of its functional significance via RNA interference (RNAi) technology, whereby the translation of a protein is prevented by selective degradation of its encoded mRNA. Down-regulation of SlTPL1 resulted in facultative parthenocarpy. Plants of SlTPL1-RNAi transgenic lines produced similar fruits which did not show any pleiotropic effects under normal conditions. However, they produced seedless fruits upon emasculation and under heat stress conditions. Furthermore, SlTPL1-RNAi flower buds contained higher levels of cytokinins and lower levels of abscisic acid. To reveal how SlTPL1 regulates facultative parthenocarpy, RNA-seq was performed to identify genes regulated by SlTPL1 in ovaries before and after fruit set. The results showed that down-regulation of SlTPL1 resulted in reduced expression levels of cytokinin metabolism-related genes, and all transcription factors such as MYB, CDF, and ERFs. Conversely, down-regulation of SlTPL1 induced the expression of genes related to cell wall and cytoskeleton organization. These data provide novel insights into the molecular mechanism of facultative tomato parthenocarpy and identify SlTPL1 as a key factor regulating these processes.
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http://dx.doi.org/10.3389/fpls.2021.672232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8174789PMC
May 2021

Melatonin regulates antioxidant strategy in response to continuous salt stress in rice seedlings.

Plant Physiol Biochem 2021 Aug 19;165:239-250. Epub 2021 May 19.

College of Agriculture, Nanjing Agricultural University, Nanjing, China; Key Laboratory of Crop Physiology Ecology and Production Management, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, China; National Engineering and Technology Center for Information Agriculture, Nanjing, China. Electronic address:

Melatonin mediates multiple physiological processes in plants and is involved in many reactions related to the protection of plants from abiotic stress. In this paper, the effect of melatonin on the antioxidant capacity of rice under salt stress was studied. Melatonin alleviated the inhibition of salt stress on the growth of rice seedlings, mainly by increasing the dry weight and fresh weight of shoots and roots. Melatonin alleviated the membrane damage caused by salt stress, which was mainly manifested by the decrease of TBARS content and the decrease of leaf and root damage. During the whole salt stress period, rice after melatonin pretreatment showed lower ROS (HO, O,OH) accumulation. In the early stage (1-3 d) of stress, the rice after melatonin pretreatment showed a strong increase in antioxidant enzyme activity, while in the later stage (5,7 d), it showed a strong increase in antioxidant content. During the whole period of salt stress, melatonin had a weak regulatory effect on AsA-GSH cycle. Through the above regulation process, the decreasing effect of melatonin on ROS content of rice under salt stress did not decrease with prolonged stress time in a short time (1-7 d). In conclusion, melatonin improved the antioxidant capacity of rice under continuous salt stress, and rice showed variable antioxidant strategies after melatonin pretreatment.
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http://dx.doi.org/10.1016/j.plaphy.2021.05.003DOI Listing
August 2021

Detection and recognition of ultrasound breast nodules based on semi-supervised deep learning: a powerful alternative strategy.

Quant Imaging Med Surg 2021 Jun;11(6):2265-2278

Department of Medical Imaging, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Background: The successful recognition of benign and malignant breast nodules using ultrasound images is based mainly on supervised learning that requires a large number of labeled images. However, because high-quality labeling is expensive and time-consuming, we hypothesized that semi-supervised learning could provide a low-cost and powerful alternative approach. This study aimed to develop an accurate semi-supervised recognition method and compared its performance with supervised methods and sonographers.

Methods: The faster region-based convolutional neural network was used for nodule detection from ultrasound images. A semi-supervised classifier based on the mean teacher model was proposed to recognize benign and malignant nodule images. The general performance of the proposed method on two datasets (8,966 nodules) was reported.

Results: The detection accuracy was 0.88±0.03 and 0.86±0.02, respectively, on two testing sets (1,350 and 2,220 nodules). When 800 labeled training nodules were available, the proposed semi-supervised model plus 4,396 unlabeled nodules performed better than the supervised learning model (area under the curve (AUC): 0.934±0.026 0.83±0.050; 0.916±0.022 0.815±0.049). The performance of the semi-supervised model trained on 800 labeled and 4,396 unlabeled nodules was close to that of the supervised learning model trained on a massive number of labeled nodules (n=5,196) (AUC: 0.934±0.026 0.952±0.027; 0.916±0.022 0.918±0.017). Moreover, the semi-supervised model was better than the average accuracy of five human sonographers (AUC: 0.922 0.889).

Conclusions: The semi-supervised model can achieve excellent performance for nodule recognition and be useful for medical sciences. The method reduced the number of labeled images required for training, thus significantly alleviating the difficulty in data preparation of medical artificial intelligence.
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http://dx.doi.org/10.21037/qims-20-12bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107344PMC
June 2021

Multi-Satellite Relative Navigation Scheme for Microsatellites Using Inter-Satellite Radio Frequency Measurements.

Sensors (Basel) 2021 May 27;21(11). Epub 2021 May 27.

School of Aeronautics and Astronautics, Zhejiang University, Hangzhou 310027, China.

The inter-satellite relative navigation method-based on radio frequency (RF) range and angle measurements-offers good autonomy and high precision, and has been successfully applied to two-satellite formation missions. However, two main challenges occur when this method is applied to multi-microsatellite formations: (i) the implementation difficulty of the inter-satellite RF angle measurement increases significantly as the number of satellites increases; and (ii) there is no high-precision, scalable RF measurement scheme or corresponding multi-satellite relative navigation algorithm that supports multi-satellite formations. Thus, a novel multi-satellite relative navigation scheme based on inter-satellite RF range and angle measurements is proposed. The measurement layer requires only a small number of chief satellites, and a novel distributed multi-satellite range measurement scheme is adopted to meet the scalability requirement. An inter-satellite relative navigation algorithm for multi-satellite formations is also proposed. This algorithm achieves high-precision relative navigation by fusing the algorithm and measurement layers. Simulation results show that the proposed scheme requires only three chief satellites to perform inter-satellite angle measurements. Moreover, with the typical inter-satellite measurement accuracy and an inter-satellite distance of around 1 km, the proposed scheme achieves a multi-satellite relative navigation accuracy of ~30 cm, which is about the same as the relative navigation accuracy of two-satellite formations. Furthermore, decreasing the number of chief satellites only slightly degrades accuracy, thereby significantly reducing the implementation difficulty of multi-satellite RF angle measurements.
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http://dx.doi.org/10.3390/s21113725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199208PMC
May 2021

Identification of the Multiresistance Gene in Oxazolidinone-Susceptible and of Pig and Feed Origins.

Pathogens 2021 May 14;10(5). Epub 2021 May 14.

College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China.

Previous studies on the prevalence and transmission mechanism of oxazolidinone resistance gene in CoNS are lacking, which this study addresses. By screening 763 CoNS isolates from different sources of several livestock farms in Guangdong, China, 2018-2020, we identified that the was present in seven CoNS isolates of pig and feed origins. Species identification and multilocus sequence typing (MLST) confirmed that seven -positive CoNS isolates were composed of five ST64- and two isolates. All -positive isolates shared similar pulsed-field gel electrophoresis (PFGE) patterns. Transformation assays demonstrated all -positive isolates were able to transfer gene to RN4220. S1-PFGE and whole-genome sequencing (WGS) revealed the presence of -carrying plasmids in size around 54.7 kb. The plasmid pY80 was 55,758 bp in size and harbored the heavy metal resistance gene and antimicrobial resistance genes, , , and (L). The regions (IS--IS) in plasmid pY80 were identified in spp. and spp. with different genetic and source backgrounds. In conclusion, this was the first report about the gene in and , and IS may play an important role in the dissemination of among different Gram-positive bacteria.
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http://dx.doi.org/10.3390/pathogens10050601DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156375PMC
May 2021